CN100458406C - MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure - Google Patents
MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure Download PDFInfo
- Publication number
- CN100458406C CN100458406C CNB200510057148XA CN200510057148A CN100458406C CN 100458406 C CN100458406 C CN 100458406C CN B200510057148X A CNB200510057148X A CN B200510057148XA CN 200510057148 A CN200510057148 A CN 200510057148A CN 100458406 C CN100458406 C CN 100458406C
- Authority
- CN
- China
- Prior art keywords
- fibre
- fiber
- sensor
- optical
- long period
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
A chemical and biological transducer is prepared by setting two long period fibre gratings, fibre covering and fibre core on one piece of fibre to form a MZ interferometer and then plating a metal film on fibre covering surface to form SPR transducer of fibre MZ interferometer structure. The sensing system comprises wide band light source, fibre, chemical and biological transducer formed as above, fibre spectrograph and computer as said transducer is set in gas or solution of chemical composition or biological molecule to be tested.
Description
Technical field
The invention belongs to Fibre Optical Sensor, biology and technical field of chemistry, be specifically related to a kind of fibre-optical microstructure MZ interference SPR chemical and biology sensor and system thereof.
Background technology
Optical fibre bio and chemical sensor are a kind of novel sensing technologies that just was born 1970 mid-nineties 90s, this kind sensor is to have the indication Ji dyestuff of identification of chemistry and biomolecule and transduction, enzyme, coenzyme, biological acceptor, antigen, antibody, nucleic acid, DNA, animal vegetable tissue or cell, microorganism, sensitive membrane such as macromolecular material, molten gel or itself, absorption, solidify or be installed in optical fiber, planar optical waveguide, on the capillary waveguide, test substance in the sample is carried out selectivity absorb (absorption) or identification, convert various optical informations again to, as ultraviolet, as seen reach the absorption of infrared light, reflection, fluorescence, phosphorescence, chemiluminescence and bioluminescence, Raman scattering, the output of signal such as optoacoustic and surface plasma body resonant vibration, thus biological or chemical information obtained.In most of sensors, optical fiber plays the transmitting effect of light, also has operative sensor to be based on measured matter directly to influence the waveguide change of properties of optical fiber to carry out chemistry and bio-sensing.
The principal feature of optical fibre bio and chemical sensor is: (1) has very high transport information capacity, reaches the monitoring of particular analysis object in the complex mixture.(2) optical wavelength that may diminish to its propagation of the fibre-optical probe diameter of sensor belongs to the same order of magnitude, is convenient to the microminiaturization of analytical equipment, and sensor is light and handy, inexpensive, durable.(3) transducing signal is a light signal, and nothing electrically contacts between the biological or chemical sample, does not influence the electrical property of biology self, detects safer, can directly insert in biological tissue, blood vessel, the cell etc., be particularly useful for real-time continuous analyzing and testing in biological living or the body.(4) no electromagnetic interference (EMI), electrical isolation can be used for inflammable and explosive occasion.(5) chemistry of optical fiber and Heat stability is good are suitable for most of chemistry and coenocorrelation; Simultaneously, optical fiber is nontoxic, does not produce waste gas and waste liquid, does not need high-voltage great-current, does not produce electromagnetic radiation, and noiseless is especially suitable to environmental protection and biomedicine.(6) Fibre Optical Sensor has interior reference effect, and measured signal is reliable and stable.(7) optical fiber has the low-loss long-distance transmissions ability that is only second to superconductor, can realize remote remote measurement.
According to the effect of optical fiber in the light sensing, optical fibre bio and chemical sensor can be divided into two classes, one class is that optical fiber is as transmission medium, solidify at fiber end face chemistry or biomolecule had optionally responsive rete, the light in the optical fiber arrive behind the end face with sensitive membrane in chemistry or biomolecule action and be absorbed part luminous energy or fluorescence excitation.Obtain the information of biological or chemical composition by detecting absorption light intensity or emitted fluorescence intensity.Second class is will fibre cladding stay one section fibre core after the corrosion, and fibre core surface cure chemistry after being corroded or bio-sensing rete produce total reflection during the fibre core of light transmission in sensing head, and produce evanescent wave.Evanescent wave passes fibre core and arrives chemistry or bio-sensing layer and be absorbed or fluorescence excitation, tests chemistry or biological information by the absorption or the fluorescence intensity of measuring light.If plate layer of metal film again having corroded on the fibre core behind the fibre cladding, can form optical fiber plasma resonance biochemical sensor.
The sensor close with the application's patent is surface plasma body resonant vibration (SPR) biology sensor.Surface plasmon resonance biosensor is molecular biosciences and chemistry, optics, electronics and the product that computer technology etc. combines, and is one of most important technology of obtaining of present molecular biology information.Introduce existing surface plasmon resonance biosensor below:
(1) ultimate principle of surface plasmon resonance biosensor
Surface plasma (SP) ripple is the electromagnetic wave of propagating along interface between metal and dielectric.The polarized light that is parallel to dividing surface when the vibration of specific wavelength impinges upon on the interface with a certain specific incident angle and when total reflection takes place, incident light will be coupled as surface plasma wave and be attenuated, the luminous energy that causes the interface total reflection significantly reduces, Here it is surface plasma body resonant vibration (SPR).SPR is very responsive to the dielectric refractive index attached to the metal surface, and refractive index is the inherent feature of all material, and the response intensity of surface plasma resonance incident angle and SPR is different and different with dielectric and adhesion amount thereof.Like this, known biological identification molecule is solidificated in metallic film surface, when the tested biomolecule that adds with its complementation, both combine and will the refractive index of metal film and solution interface be risen, thereby cause the change of SPR resonance angle or wavelength, just can realize quantitative or qualitative detection the target biological molecules of complementation by detecting resonance incident angle or wavelength change.
(2) type of existing surface plasmon resonance biosensor
According to the difference of optical waveguide coupled device, spr sensor can be divided into prism-type, metal grating type and optical-fiber type.Present most sensor is the prism-type sensor, and the prism-type device that is used to produce attenuated total reflectance has Otto and Kretschmann type, and based on the Kretschmann type, as shown in Figure 1.This device is the bottom that the metallic film 11 of tens nanometer thickness is directly overlayed prism 12, medium 13 to be detected is below metal film, light is injected prism, its evanescent wave sees through metallic film and causes surface plasma body resonant vibration (SPR) at the interface at another, and prism can be a right angled isosceles triangle or hemispheric.In order to improve sensitivity, the higher optical material of normal selective refraction rate is made prism, but the surface strength of this optical material and less stable easily form oxide film in air.
People such as Tiefenthaler had proposed the surface plasmon resonance biosensor of metal diffraction grating as the light wave coupling element in 1987, but the making of metal grating acquires a certain degree of difficulty.People such as Nikitin P I had developed the metal diffraction grating surface plasmon resonance biosensor based on Schottky junction structure in 1997, utilize microelectronic processing technique on silicon chip 21, to plate the golden film grating 22 of the thick period profile of one deck 40nm, cover one deck biomolecule sensitive membrane 23 again, as shown in Figure 2.After the molecular biosciences film absorbs tested biomolecule, its refractive index changes, the light field of metal grating diffraction and spectral distribution also produce corresponding variation, convert the variation of light signal to electric signal output, detect Surface enhanced raman spectroscopy with spectroanalysis instrument simultaneously.The voltage that utilization obtains-angle curve and Raman line realized the test and the analysis of biomolecular reaction mechanism and process.
The sensor information of prism-type and metal grating type surface plasmon resonance biosensor is not an optical fiber, needs flow cell, and is higher to using environmental requirement.1993, people such as Jorgenson proposed the optical-fiber type surface plasmon resonance biosensor, and two kinds of sensing arrangements are arranged, and as shown in Figure 3, a kind of is online conveying type (a), and another kind is end reflection formula (b).Online conveying type optical fiber biology sensor is that the covering 31a in one section silica fibre is peelled off, and deposition one deck high-reflectivity metal film 33a covers one deck biomolecule sensitive membrane 34a again on metal film on fiber cores 32a.The diameter of optical fiber is generally 0.3mm, but the angular range of transmission ray is 78.5～90 ° in the optical fiber.In this angular range, inner total reflection takes place in light on the interface of fibre core and covering, and the evanescent wave that sees through the interface causes surface plasma in metal film, and produces resonance on certain angle and wavelength.Endpiece at optical fiber detects output light intensity and Wavelength distribution, but the concentration of quantitative test biomolecule and variation thereof.
The building method of end reflection formula SPR optical fiber biosensor is the metal film 33b that deposition one layer thickness reaches 300nm on an end face of optical fiber, fibre cladding 31b about this end one segment length 5mm is peelled off, and, on metal film, cover one deck biomolecule sensitive membrane 34b at the metal film about deposition 50nm on the fiber cores 32b.When satisfying the SPR resonant condition, the light in the optical fiber will produce resonance and be absorbed; When light transmission is reflected back along incoming road during to end face, be transferred to fiber spectrometer through resonance absorption decay back again and detect.Behind molecule sensitive membrane organism-absorbing molecule, its refractive index changes, thereby changes the decay light intensity and the resonant wavelength of light in the optical fiber.Change mechanism and the quantity that gets final product detectable biomolecule according to this.This mode has been omitted flow cell, can realize remote measurement in the certain distance scope.
All in all, surface plasmon resonance biosensor is based on prism-type at present, and optical-fiber type and metal grating type are auxilliary.
(3) have SPR sensor apparatus system characteristics now
Surface plasmon resonance biosensor has advantages such as non-destructive, high sensitivity and real-time online detection, is being widely used aspect the detection of microorganism, blood, DNA, antigen-antibody, toxic gas etc. and the analysis.The advantage of spr sensor and instrument system thereof is: (1) biologic specificity is strong, can the process of biomolecular reaction be detected; (2) simple to operate, measuring speed is very fast; (3) complete machine Miniaturizable; (4) be fit to microcell and surface analysis, also can carry out analysis of the molecular structure; (5) sensitivity is very high, than highly sensitive 1～2 order of magnitude of evanescent wave fluorescence detecting system.
Also there are some defectives in such sensor and system: (1) prism-type sensing head complexity and volume are big.The making of optical fiber sensor head is loaded down with trivial details and very difficult, needs to remove covering by chemical corrosion or abrasion method, and the removal position or the size of covering are difficult to accurate control.The making of metal diffraction grating type sensing head is also very difficult.(2) system stability is relatively poor: no matter be wavelength-modulated or angular modulation method, the signal of direct detection all is a light intensity, and is monochromatic light road light intensity.The fluctuation of light source, light path and Circuits System and interference are very big to the influence of sensor-based system, have reduced reliability and accuracy.In the intensity detection of existing monochromatic light road, this influence is difficult to eliminate.(3) interference of stray light is big: in prism-type, metal grating type and three kinds of spr sensors of optical-fiber type, spuious luminous energy enters light path and detector.When extraneous parasitic light changed, the signal that detects also changed, and therefore needed the shielding parasitic light.(4) for the optical-fiber type sensor, behind chemical corrosion or the abrasion covering, coarse interface can appear, and be difficult to obtain level and smooth fibre core optical surface, thereby influence total reflection characteristic at random, produce big noise signal, reduce signal to noise ratio (S/N ratio) greatly.(5) data do not have fault-tolerant ability: the data that existing sensor-based system obtains do not have redundant information, when larger interference and mistake occurring, have a strong impact on test or precision of analysis.(6) a little less than the remote measurement ability: prism-type and metal grating type spr sensor are difficult to realize remote measurement by optical fiber; The used optical fiber of optical-fiber type spr sensor mainly is the bigger multimode optical fiber of core diameter (the making difficulty of little core optical fibers sensor at present, even can't make), general only is to utilize one section about optical fiber of tens centimetres as sensing head, is difficult to make full use of the superiority of optical fiber.(7) sensor-based system complex structure and huge, the operation underaction, prism-type and metal grating type spr sensor all need flow cell.Environmental suitability is relatively poor, and temperature variation is big to the influence of test result, is subjected to the interference of extraneous vibration also big, aims at very difficulty of debugging, and existing optical-fiber type sensor also is like this.
These are not enough and defective remains new technology and method and overcome and improve, so that this biological chemistry sensing technology can obtain the higher generalization application.
Summary of the invention
Purpose of the present invention just is at the prior art above shortcomings, a kind of fibre-optical microstructure MZ (Mach-Zehnder is provided, Mach-Zehnder) interference SPR (Surface Plasmon Resonance, surface plasma body resonant vibration) chemistry and biology sensor and system thereof, the metal film of the responsive rete of the needed MZ interferometric device of this sensor, two light paths, chemistry or biomolecule, SPR sensing all is integrated on one section optical fiber, make the complete fiberize of whole sensor, structure miniization, volume is little.Sensor-based system just can be measured the concentration of chemical constitution or biomolecule by measuring the variation of interference fringe amplitude or its spectrum distribution, surface plasma body resonant vibration absorbing wavelength, obtains higher accuracy of detection and sensitivity.Sensor-based system can be realized remote measurement based on optical fiber link simultaneously, and system architecture is simple, and debugging is convenient, is not subjected to the influence of parasitic light, and test result is reliable and stable.
Technical scheme of the present invention is as follows:
Fibre-optical microstructure MZ interference SPR chemical and biology sensor, it be on an optical fiber at a distance of certain distance with two long period fiber grating, as two coupling mechanisms, the about 3-70cm of distance between two long period fiber grating; These two long period fiber grating have close coupled wavelength, bandwidth and coupling efficiency, and the coupling efficiency of long period fiber grating resonance central wavelength is about 2-10dB; At the optical fiber between two long period fiber grating fibre cladding outside fiber cores and the fiber cores is arranged, these two long period fiber grating and between fiber cores and fibre cladding constitute an optical fiber MZ (Mach-Zehnder) interferometer.In this optical fiber MZ interferometer, between two long period fiber grating whole section or segment section optical fiber have only fiber cores and fibre cladding, have removed the outer protective finish of fibre cladding, have only the about 3-70cm of total length of this part optical fiber of fiber cores and fibre cladding; Fibre cladding surface at this part optical fiber that has only fiber cores and fibre cladding is coated with layer of metal film, and as the carrier of surface plasma body resonant vibration (SPR), the thickness of metal film is about 30-200nm.This has just constituted fibre-optical microstructure MZ interference SPR chemical and biology sensor, utilizes total reflection, surface plasma body resonant vibration (SPR) and the interference of light characteristic of fibre cladding to wait the information of obtaining biomolecule and chemical constitution.
Further, in order to improve selectivity (specificity) and the sensitivity of sensor to particular chemical composition or biomolecule, also can be solidified with absorption or the responsive rete of a layer thickness greater than 3nm on above-mentioned metallic film surface again, this absorption or responsive rete have optionally absorption or sensitivity characteristic to tested chemical constitution or biomolecule.About 125～1000 microns of the external diameter of whole sensor.
The sensing principle of this sensor is to utilize the fibre cladding light in the fiberize MZ interferometer to wait sensing chemistry or biological information at the interface total reflection, surface plasma body resonant vibration and interference of light characteristic: when the light that transmits in (1) fiber core arrives a long period fiber grating, part light continues transmission forward in fibre core, and another part light is coupled in the covering by long period fiber grating and transmits.(2) fibre cladding light is in the total reflection of generation at the interface of fibre cladding and metallic diaphragm and transmission forward in covering, and the total reflection at the interface of fibre cladding and metallic diaphragm will produce evanescent wave.This evanescent wave passes covering interface and metallic diaphragm, enters into a tiny area of the responsive rete of tested biomolecule and chemical constitution or its solution, gas.(3) if the refractive index of the incident angle of fibre cladding light, wavelength and fibre cladding, metal membrane material and thickness thereof, biology and the solution of chemical constitution or the parameters such as refractive index of its gas or its responsive rete satisfy the phase matching resonant condition, when the evanescent wave at fibre cladding and metal interface place passes metallic diaphragm, to produce surface plasma body resonant vibration, the energy of evanescent wave is shifted the energy into the metallic film surface plasma wave by resonance coupling, thereby causes the energy of evanescent wave to be attenuated.(4) evanescent wave after being attenuated returns fibre cladding and continues transmission, produces total reflection, evanescent wave, metal surface plasma resonance absorption at other point again.(5) when the light in the fibre cladding arrives another long period fiber grating, a part of light in the fibre cladding is coupled in the fiber core and goes, and interferes with a part of combiner that transmits in fiber core and generation always.In the two-beam of produce interfering, the light in the fibre core is not subjected to the influence of chemical constitution or biomolecule, but the amplitude of light and phase place are subjected to the influence of biomolecule or chemical constitution concentration in the fibre cladding because of the effect of surface plasma body resonant vibration and evanescent wave.(6) the concentration difference of the kind of biomolecule and quantity thereof or chemical constitution, also different with the refractive index of the biology of metallic diaphragm outside surface contact and chemical solution or its gas or its responsive rete, thereby influence the matching condition of metallic film surface plasma resonance, damping capacity, phase place, resonance optical wavelength and the resonance angle of fibre cladding evanescent wave are changed, thereby influence the amplitude and the distribution thereof of interference signal light.Variation relation between them is determined.(7) amplitude by measuring interference signal light and distribution thereof, surface plasma body resonant vibration wavelength change just can be measured the concentration of biomolecule or chemical constitution, the sensing of realization biomolecule or chemical constitution.
The sensor-based system that is formed by above-mentioned fibre-optical microstructure MZ interference SPR chemical and biology sensor includes: wideband light source, optical fiber, fibre-optical microstructure MZ interference SPR chemical and biology sensor, fiber spectrometer and computing machine; Wherein, the output of wideband light source links to each other with an optical fiber end of biology sensor with optical fiber microstructure MZ interference SPR chemical by optical fiber, fibre-optical microstructure MZ interference SPR chemical links to each other by the input of optical fiber with fiber spectrometer with another optical fiber end of biology sensor, fiber spectrometer links to each other with computing machine by data-interface (as USB, RS232, GPIB etc.), and sensor is placed in the solution or gas of tested chemical constitution or biomolecule.Like this, light in the optical fiber is through the coupling of long period fiber grating, repeatedly total reflection and metallic film surface plasma resonance absorb, and are coupled when arriving another long period fiber grating to close bundle formation interference light signal again, and interference light signal is transferred to fiber spectrometer through optical fiber again; Fiber spectrometer obtains the spectral amplitude ratio and the distribution thereof of interference light signal, and by data-interface the interference light signal data is delivered to computing machine, by computing machine according to the interference light signal data computation obtained and the measured value that demonstrates chemical constitution concentration or biomolecule.
The interference light signal data that obtained by above-mentioned sensor-based system have multiple information parameter and distribution thereof, as amplitude, additive phase, interference fringe and distribution thereof etc., all can carry out analytical calculation by computing machine.It is different that these different informations parameter are subjected to the influence of biomolecule or chemical constitution, has different response forms and relevance, is multidimensional information, can measure chemical constitution or biomolecule with any one-dimension information wherein.In data acquisition, transmission and processing procedure, the lost part data still can correctly obtain measurement result, and sensor-based system has certain fault-tolerant ability.
The advantage of this sensor and sensor-based system:
This sensor has the superiority of general Fibre Optical Sensor, as be subjected to electromagnetic interference (EMI) little, sensor is small and light, stability is high, nontoxic insulation, and no current passes through, realize that easily the multiple tracks polycomponent detects simultaneously and analyzes, can have height selectivity and susceptibility, be suitable for strong acid, highly basic, High Temperature High Pressure, rugged surroundings such as inflammable and explosive, especially when biomedical sector is used in bulk measurement, have tight security.In addition, this sensor and sensor-based system also have unique advantage, comprise: (1) this sensor construction uniqueness is ingenious, not additional any non-optical fibre device, only utilize ordinary optic fibre and on two long period fiber grating just realized the MZ interferometer, directly plate metallic diaphragm on the fibre cladding surface of fiberize MZ interferometer and just realized optical fiber SPR sensor, also can on metallic diaphragm, solidify again chemical constitution or biomolecule had and optionally absorb or responsive rete.The complete fiberize of MZ optical interference circuit system and whole sensor thereof, structure miniization, external structure is succinct, and volume is little, and powerful.This is the maximum innovative point of this sensor.(2) sensor-based system is not subjected to the influence of parasitic light.This sensor-based system can be measured the interference spectrum signal, and parasitic light and flashlight do not satisfy coherent condition.Therefore, parasitic light does not influence measurement result.(3) the fabricating quality controllability of sensor is good, and the flashlight noise is little.Each part of this sensor and manufacturing process thereof all are accurately controlled, and fabricating quality is guaranteed easily.Sensing section optical fiber need not corrode, and the total reflection interface is smooth optical surface, and the noise of flashlight is little.And the sensor fibre of existing fiber spr sensor needs to handle through chemical corrosion or abrasion, and the total reflection interface is not smooth optical surface, can produce very big noise.(4) whole measuring system can realize remote measurement based on the ordinary optic fibre link, and the formation of system and assembling only need welding optic fibre, and debugging is simplified, the reliability height.Simultaneously, sensor-based system has certain fault-tolerant ability.(5) measuring accuracy and highly sensitive, the signal to noise ratio (S/N ratio) of this sensor are higher than existing optical fiber biology sensor, if measure biomolecule or chemical constitution with the additive phase method, can reach high measuring accuracy and sensitivity.
In a word, this sensor and system architecture uniqueness thereof, microminiaturized, fiberize, utilize evanescent wave, metal surface plasma resonance body and the interference of light characteristic of fibre cladding to measure biomolecule or chemical constitution, a complete microstructure MZ interferometer and biology and chemical sensor on one section optical fiber, have been realized, system sensitivity height, good reliability.
Description of drawings
Fig. 1 is a prism-type SPR biochemical sensor structural drawing;
Fig. 2 is a metal grating type SPR biochemical sensor structural drawing;
Fig. 3 is existing optical fiber biochemical sensor structural drawing;
Fig. 4 is the structural drawing of the sensor that the present invention relates to;
Fig. 5 is the structural drawing of the sensor-based system that the present invention relates to;
Fig. 6 is the interference signal spectrogram that the sensor-based system computing machine that the present invention relates to obtains.
Embodiment
Below in conjunction with accompanying drawing, concentration with the measurement glucose solution is the enforcement that example illustrates the sensor and system, the formation of the biomolecule of gas or other solution form and chemical constitution measuring system and sensor thereof similarly, difference is just solidified different chemistry of one deck or biomolecule sensitivity rete on the metallic diaphragm fibre cladding outside.
Referring to Fig. 4; the structure of this sensor is formed to be had: the absorption of optical fiber coating 1, fibre cladding 2, fiber cores 3, long period fiber grating LPFG14, metallic diaphragm 5, chemistry or biomolecule or responsive rete 6, long period fiber grating LPFG27; protective sleeve 8; solidify glue 9, plastics transition buffer cover 10.Its method for making is: (1) removes a bit of plastic protective coating of a general single mode fiber (fiber optic materials is quartz or plastics, the about 9 μ m of its optical fiber core diameter, the about 125 μ m of fibre cladding diameter); (2) be about the laser instrument of 240nm and this section optical fiber surface that plastic protective coating has been removed in long period fiber grating mask plate (cycle of mask plate decides according to necessary wavelength) irradiation with wavelength, write a long period fiber grating that is about 2.5cm, an its resonance centre wavelength drops near the 760nm, the about 3.5dB of the coupling efficiency of resonance central wavelength, the about 18nm of bandwidth; (3) remove one section plastic protective coating again at the about 13cm of segment distance place from the long period fiber grating that has write, shine this section optical fiber surface of having removed plastic protective coating with laser instrument identical and fiber grating mask plate, write a similar long period fiber grating (also available CO2 laser instrument writes this two long period fiber grating) that is about 2.5cm again with writing first long period fiber grating; (4), be coated with for optical fiber again with the optical fiber coating machine and apply plastic protective coating in two long period fiber grating positions that write; (5) between two long period fiber grating, the plastic protective coating of the about 9.5cm of a segment length is removed, and cleaned this section fibre cladding of having removed plastic protective coating with ethanol and biological reagent; (6) removing on this section fibre cladding of plastic protective coating, plating metallic gold or the silverskin of the about 60nm of a bed thickness, making its surface plasma body resonant vibration wavelength be about 760nm; (7) metallic gold or the about 150nm of silverskin surface cure one bed thickness glucose molecule is had the responsive rete that selectivity absorbs, also can not solidify this sensitivity rete; (8) this section optical fiber that will make long period fiber grating, plastic protective coating, metallic gold or silverskin, the responsive rete of glucose molecule with curing glue in both ends paste porose protective sleeve; each loads onto a plastics transition buffer cover respectively at the protective sleeve two ends, can make of the packaged both ends of glue to finish again.This protective sleeve can permeate tested glucose solution, simultaneously glucose solution is contacted with the metal film or the glucose-sensitive rete of optical fiber sensor head.This protective sleeve also can, and directly this sensor is placed in the glucose solution, also can realize detecting.
The formation of this sensor-based system as shown in Figure 5.Centre wavelength 750nm, bandwidth output to single-mode fiber B greater than the wideband light source A of 60nm through coupling, arrive fibre-optical microstructure MZ interference SPR chemical and biology sensor C behind a section single-mould fiber B.In sensor C, the part of luminous energy is transmitted the influence that is not subjected to glucose molecule in the solution in fiber cores near the 760nm, and another part luminous energy is coupled to and goes in the fibre cladding and transmit in covering; Light in the fibre cladding absorbs at the evanescent wave of generation at the interface and the surface plasma body resonant vibration of fibre cladding and metallic diaphragm, and its SPR resonance absorption is subjected to the influence of glucose molecule concentration; When running into next long period fiber grating, the part of fibre cladding light enters fibre core once more, interferes with a part of light generation of fiber cores, and interference light signal transmits in fiber cores, and interference light signal is transferred to high-precision fiber spectrometer D by optical fiber.Fiber spectrometer D obtains interference light signal and distributed data thereof, by data-interface GPIB the interference light signal data is delivered to computing machine E, is obtained and shown the concentration of glucose in the solution by COMPUTER CALCULATION.The interference signal spectrogram data that computing machine obtains as shown in Figure 6.Its measurements and calculations process is the sensitivity coefficient of first calibration sensor to glucose solution, the concentration of testing glucose solution then.During test, this sensor is placed in the tested glucose solution, and the computing machine in the sensor-based system obtains the interference signal spectroscopic data, and calculates the concentration of tested glucose solution according to the sensitivity coefficient of demarcating, thereby realizes the sensing of glucose.
In the present embodiment, the wavelength resolution of fiber spectrometer can reach 0.02nm, and the minimum amplitude measured value is-65dBm, and the spacing of interference signal spectrum striped is 1.52nm, with the resolution of crest meter algorithm measurement glucose solution concentration less than 6.1 μ g/ml.If when measuring with the additive phase method, the resolution of measuring glucose solution concentration can reach every milliliter of submicrogram level.
Claims (4)
1, fibre-optical microstructure Mach-Zehnder interference SPR chemical biology sensor, it is characterized in that: it has two long period fiber grating at a distance of certain distance on an optical fiber, these two long period fiber grating have close coupled wavelength, bandwidth and coupling efficiency; The coupling efficiency of long period fiber grating resonance central wavelength is 2-10dB; Optical fiber between these two long period fiber grating has the fibre cladding outside fiber cores and the fiber cores; These two long period fiber grating and between fiber cores and fibre cladding constitute a Mach-Zehnder interferometer; There is layer of metal film between these two long period fiber grating whole section or the fibre cladding surface of segment section optical fiber, are solidified with chemical constitution or biomolecule had on metal film optionally to absorb or responsive rete.
2, fibre-optical microstructure Mach-Zehnder interference SPR chemical biology sensor according to claim 1, it is characterized in that: the distance between two long period fiber grating is 3-70cm, the thickness of described metal film is 30-200nm, the total length of metal film is 3-70cm, and the thickness of described absorption or responsive rete is greater than 3nm.
3, fibre-optical microstructure Mach-Zehnder interference SPR chemical biology sensor according to claim 1; it is characterized in that: outside above-mentioned this section optical fiber of making long period fiber grating, fibre cladding, metal film, biomolecule or chemical constitution absorption or responsive rete, be with a protective sleeve; aperture is arranged on the protective sleeve; the two ends of protective sleeve are pasted together by solidifying glue and this sensor fiber, at the both ends of protective sleeve a transition buffer cover are arranged respectively.
4, the sensor-based system that forms by claim 1 or 2 described sensors, it is characterized in that: it comprises wideband light source, optical fiber, fibre-optical microstructure Mach-Zehnder interference SPR chemical and biology sensor, fiber spectrometer and computing machine; This wideband light source and fiber spectrometer are connected respectively to two optical fiber end of fibre-optical microstructure Mach-Zehnder interference SPR chemical and biology sensor, fiber spectrometer is connected to computing machine by data-interface, and sensor places the gas or the solution of tested chemical constitution or biomolecule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510057148XA CN100458406C (en) | 2005-07-01 | 2005-07-01 | MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510057148XA CN100458406C (en) | 2005-07-01 | 2005-07-01 | MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1712928A CN1712928A (en) | 2005-12-28 |
| CN100458406C true CN100458406C (en) | 2009-02-04 |
Family
ID=35718633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200510057148XA Expired - Fee Related CN100458406C (en) | 2005-07-01 | 2005-07-01 | MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100458406C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102095719A (en) * | 2010-12-30 | 2011-06-15 | 浙江工业大学 | Optical fiber type sensor system based on surface plasma resonance and stimulated Raman scattering |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101395463B (en) * | 2005-09-22 | 2011-05-25 | 皇家飞利浦电子股份有限公司 | Luminescence sensor comprising at least two wire grids |
| CN101190829B (en) * | 2006-11-29 | 2010-09-01 | 河南农业大学 | Method for preparing optical fiber SPR sensor gold film by microwave radiation assisted chemical plating |
| CA2779356A1 (en) * | 2009-11-05 | 2011-05-12 | Waterford Institute Of Technology | A nanohole array biosensor |
| CN101936879B (en) * | 2010-07-28 | 2011-11-30 | 山东大学 | Photoacoustic spectroscopy gas detecting system based on Mach-Zehnder interferometer |
| CN102062899B (en) * | 2010-11-10 | 2012-03-21 | 华中科技大学 | Optical fiber sensing head with nano grid structure |
| CN102735653B (en) * | 2011-04-14 | 2014-07-16 | 国家纳米科学中心 | Biological detection method by utilization of surface plasma resonance biosensor |
| CN102507503B (en) * | 2011-10-24 | 2013-11-27 | 天津大学 | Fiber surface plasmon resonance glucose sensor with temperature self-compensation |
| CN103149153B (en) * | 2013-01-30 | 2015-04-01 | 中国科学院光电技术研究所 | Test analysis method for super diffraction material light-transfer characteristics |
| CN104359868B (en) * | 2014-11-07 | 2017-04-05 | 中国计量学院 | Based on the inclined optical fiber grating surface plasma resonance biosensor that M Z interfere |
| CN104596958B (en) * | 2015-01-19 | 2017-06-09 | 电子科技大学 | The analysis method of the biochemical sensor based on series connection LPWG |
| CN106442419B (en) * | 2016-09-06 | 2019-01-01 | 大连理工大学 | Self compensation SPR optical fiber biochemical sensor |
| CN106770032A (en) * | 2016-12-07 | 2017-05-31 | 大连理工大学 | Phase detection type optical fiber biochemical sensitive system |
| CN108444547A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of mercury ion content is detected based on genetic chip and silver nano-grain |
| CN108444538A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of mercury ion content is detected based on aldehyde radical chip and gold nano grain |
| CN108444542A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of silver ion content is detected based on gold silver nanometer particle |
| CN108507623A (en) * | 2018-06-05 | 2018-09-07 | 天津大学 | The environment network of silver ion content is detected based on gold nano grain |
| CN108444545A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of silver ion content is detected based on aldehyde radical chip and silver nano-grain |
| CN108519128A (en) * | 2018-06-05 | 2018-09-11 | 天津大学 | The environment network of mercury ion content is detected based on genetic chip and gold nano grain |
| CN108444539A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of silver ion content is detected based on silver nano-grain |
| CN108444544A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of mercury ion content is detected based on silver nano-grain |
| CN108444541A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of silver ion content is detected based on aldehyde radical chip and gold nano grain |
| CN108444548A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of mercury ion content is detected based on gold nano grain |
| CN108444546A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of silver ion content is detected based on genetic chip and silver nano-grain |
| CN108444543A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of mercury ion content is detected based on gold silver nanometer particle |
| CN108444540A (en) * | 2018-06-05 | 2018-08-24 | 天津大学 | The environment network of mercury ion content is detected based on aldehyde radical chip and silver nano-grain |
| CN108507624A (en) * | 2018-06-05 | 2018-09-07 | 天津大学 | The environment network of silver ion content is detected based on genetic chip and gold nano grain |
| CN108801941A (en) * | 2018-08-27 | 2018-11-13 | 电子科技大学 | Gas identification fibre optical sensor based on metal-organic framework material and recognition methods |
| CN109632720A (en) * | 2018-12-27 | 2019-04-16 | 北京信息科技大学 | A kind of Na ion concentration fibre optical sensor |
| CN109991192B (en) * | 2019-04-16 | 2021-07-27 | 重庆理工大学 | Michelson interference type hydrogen sulfide sensor based on cladding coating sensitive film |
| CN113916837B (en) * | 2021-11-17 | 2023-05-12 | 重庆三峡学院 | Optical fiber V-groove type cladding SPR curvature sensor capable of identifying direction and manufacturing method thereof |
| CN117452175A (en) * | 2023-12-20 | 2024-01-26 | 国科大杭州高等研究院 | Micro-area bandwidth testing system and method for semiconductor photoelectric device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6021240A (en) * | 1997-10-02 | 2000-02-01 | F&S, Inc. | Optical sensor activation device |
| US6058226A (en) * | 1997-10-24 | 2000-05-02 | D-Star Technologies Llc | Optical fiber sensors, tunable filters and modulators using long-period gratings |
| US20050002606A1 (en) * | 2001-09-19 | 2005-01-06 | James Stephen Wayne | Optical transmission device |
-
2005
- 2005-07-01 CN CNB200510057148XA patent/CN100458406C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6021240A (en) * | 1997-10-02 | 2000-02-01 | F&S, Inc. | Optical sensor activation device |
| US6058226A (en) * | 1997-10-24 | 2000-05-02 | D-Star Technologies Llc | Optical fiber sensors, tunable filters and modulators using long-period gratings |
| US20050002606A1 (en) * | 2001-09-19 | 2005-01-06 | James Stephen Wayne | Optical transmission device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102095719A (en) * | 2010-12-30 | 2011-06-15 | 浙江工业大学 | Optical fiber type sensor system based on surface plasma resonance and stimulated Raman scattering |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1712928A (en) | 2005-12-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100458406C (en) | MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure | |
| US7295294B2 (en) | Optical waveguide sensor and measuring apparatus using said optical waveguide sensor, and measuring method using a sensor | |
| Homola et al. | Surface plasmon resonance sensors | |
| Potyrailo et al. | Optical waveguide sensors in analytical chemistry: today’s instrumentation, applications and trends for future development | |
| Melendez et al. | A commercial solution for surface plasmon sensing | |
| EP2449363B1 (en) | Optical sensing device and method for detecting samples | |
| CN100561198C (en) | Fibre-optical microstructure Michelson interfere type surface plasma resonance chemistry and biology sensor and system | |
| Liu et al. | Wavelength-modulation surface plasmon resonance sensor | |
| US20050018949A1 (en) | Multiple array surface plasmon resonance biosensor | |
| Chen et al. | Temperature-compensating fiber-optic surface plasmon resonance biosensor | |
| CN100432656C (en) | Distributed optical fiber mode coupling biochemical sensor, optical fiber link, and sensing system | |
| CN105334190B (en) | The Bragg gratings biochemical sensor and method of fiber core and clad interface | |
| CN104596941A (en) | Composite maximum-dip-angle fiber bragg grating biochemical sensor and production method thereof | |
| CN102095719A (en) | Optical fiber type sensor system based on surface plasma resonance and stimulated Raman scattering | |
| Wang et al. | Research and application of multi-channel SPR sensor cascaded with fiber U-shaped structure | |
| CN105403535A (en) | Fiber cladding surface Bragg grating biochemical sensor and making method thereof | |
| CN100451609C (en) | Interference evanescent wave chemical and biological sensor and system with fibre-optical Michelson | |
| Kim et al. | Fiber-optic surface plasmon resonance for vapor phase analyses | |
| CN101377527A (en) | Optical fiber voltage measuring instrument | |
| CN1712929A (en) | MZ interference evanescent wave chemical and biological sensor and system with fibre-optical microstructure | |
| Chen et al. | Detection of aflatoxin b 1 in food based on smf taper combined with fiber loop ring down technique | |
| CN100432657C (en) | Optical fiber mode coupling biochemical sensor, sensing device and sensing system | |
| CN100567957C (en) | A kind of many optical fiber surface plasmon resonance probes with temperature correction | |
| CN111928880A (en) | Mach-Zehnder interference optical fiber based on surface plasma effect and sensor thereof | |
| CN2911670Y (en) | Multiple optical fibers SPR probe having temp correction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090204 Termination date: 20110701 |