CN100464692C - Method and apparatus for obtaining tissue micro tomography image and spectrum - Google Patents
Method and apparatus for obtaining tissue micro tomography image and spectrum Download PDFInfo
- Publication number
- CN100464692C CN100464692C CNB200610118100XA CN200610118100A CN100464692C CN 100464692 C CN100464692 C CN 100464692C CN B200610118100X A CNB200610118100X A CN B200610118100XA CN 200610118100 A CN200610118100 A CN 200610118100A CN 100464692 C CN100464692 C CN 100464692C
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- tissue
- signal light
- confocal system
- spectrum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention relates to a method for obtaining the organism microscope chromatography image and spectrum based on optical fiber focus system, wherein the white light is the lighting resource; the focus system lights the organism and obtains the light signal carrying the organism signal; the invention uses two methods to receives the back toward scattering signal; via signal processing, the computer displays the chromatography image and spectrum of organism, and controls the scanning device, to compare the cell shape and spectrum of normal organism, to diagnose the cancer.
Description
Technical field
The present invention relates to a kind of biomedical method and apparatus that detects, specially refer to and utilize the detection of optical fiber confocal system to organize backscatter signal light, detector receives micro tomography image and spectral information respectively, and assists the early diagnosis that carries out cancer by tissue image and spectrum that computer shows.
Background technology
The investigation report that World Health Organization (WHO) announced as far back as 2002 just shows: the whole world has 6,000 every year, and the people dies from cancer more than 000, new cases of cancer more than 10,000,000 occurs.Main cause that causes cancer high incidence and mortality rate is that existing medical detection means can't or detect effectively or to make a definite diagnosis cancer early stage.It is very necessary to develop the early stage technology of non-invasive, live body and real-time detection cancer.The burnt reflection of copolymerization micro-imaging art can be obtained the information of the different tomographies of hundreds of micrometer range in the tissue by horizontal two-dimensional scan and vertical one-dimensional scanning, reconstructs the tomographic map of organizing with micron dimension resolution.Along with rapid scanning, microfabrication and development of fiber technology occur in conjunction with novel (optical fiber) Laser Scanning Confocal Microscope of the burnt micro-imaging technique of copolymerization.It can be real-time, obtains tissue external morphology information with no damage.At present, a subject matter facing of novel (optical fiber) Laser Scanning Confocal Microscope is: it only provides histiocyte and nuclear metamorphosis information.But the deformation of cancerous issue cell is the process of a longer-term for the biochemistry composition variation of tissue.The visible reflectance spectrum characteristic that experiment showed, normal tissue cell and cancerous issue cell has remarkable difference.Cancerous issue does not produce when significantly organizing deformation in early days as yet, just contains abundant deoxyhemoglobin in the cell, can cause the remarkable change of reflectance spectrum, can be used for diagnosing for early stage canceration.Therefore, except that the micro-deformation data of collecting cancerous issue, be necessary to obtain the spectrum of biochemical composition, in time find to organize the early stage variation of biochemical composition, so that future cancer is implemented diagnosis comprehensively and timely according to spectrum.
Summary of the invention
Can not provide the change information of organizing inner cell and nuclear metamorphosis information and biochemical composition simultaneously in order to overcome prior art, the present invention proposes a kind ofly to obtain tissue micro tomography image and spectrographic device and using method thereof based on the optical fiber confocal system, can nondestructively in time find the variation of organization internal cell and nuclear metamorphosis and biochemical composition, checking for cancer is early stage provides strong help.
In order to solve its technical problem, technical scheme of the present invention is based on the optical fiber confocal system and proposes a kind of tissue micro tomography image and spectrographic device of obtaining, and is characterized in that it comprises:
One white light source is used to provide illumination light;
One collimation lens and a coupled lens is used to adjust illuminating bundle,
One by single-mode fiber and the optical fiber confocal system and the signal beams of popping one's head in and constituting;
A beam splitter realizes that the backscatter signal light that the optical fiber confocal system receives on the tissue inner fiber end face conjugate point separates with illumination light;
A beam splitter is realized the separation of flashlight;
One photomultiplier tube detectors that is made of collecting lens, pin hole and photomultiplier tube is used to receive the resultant signal light intensity;
A ccd detector that is made of transmission grating or balzed grating,, collecting lens and line array CCD is used to receive the signal light intensity of each wave band correspondence after transmission grating or the balzed grating, beam split;
One computer is used to handle signal, demonstration tissue figure information and the spectral information that is obtained by detector and sends the scan control instruction.
The probe of described optical fiber confocal system is drawn together scanning means and imaging lens group, and described scanning means is the three-dimensional scanner of being made up of the axial focussing mechanism of mini optical fibre scanning device or micro electronmechanical optical beam scanner and gas or liquid.
A kind of using method of obtaining tissue micro tomography image and spectrographic device, characteristics are that step is:
1. white illumination light is imported the optical fiber confocal system that single-mode fiber and probe constitute;
2. assemble on 0-250 μ m degree of depth in tissue through the white light of optical fiber confocal system, according to the burnt micro-imaging principle of optical fiber copolymerization, the optical fiber confocal system is the backscatter signal light on the tissue receiving inner fiber end face conjugate point only;
3. above-mentioned by the optical fiber confocal system receive organize backscatter signal light on the inner fiber end face conjugate point by optical fiber and beam splitter after enter photomultiplier tube detectors and ccd detector respectively, photomultiplier tube receives the resultant signal light intensity, and line array CCD receives the signal light intensity of each wave band correspondence after transmission grating or balzed grating, beam split;
4. described flashlight is imported Computer Processing after opto-electronic conversion, amplification and collection, the image of computer display organization and spectrum also send the scan control instruction.
Utilize image and spectral detection to organize whether early stage canceration takes place; The contrast cell of normal structure and the spectrum of karyomorphism and biochemistry composition can detect to organize according to the variation and the spectrographic variation of biochemical composition of cell and nuclear geometric shape whether early stage canceration takes place.
It is defocused to scattering imaging technique and spectrum detection technique that the present invention combines the optical fiber copolymerization, proposition is applicable to obtains organized layer's exploring and spectrographic device and using method thereof, can obtain the three-dimensional configuration image of biological tissue micron number magnitude in real time, and the spectral information of biochemical composition in the tissue, determine to organize the variation of inner cell and nuclear metamorphosis and biochemical composition according to image and spectrum.This has just solved the technical problem that present technology can not be obtained the variation of organizing inner cell and nuclear metamorphosis and biochemical composition simultaneously.
Description of drawings
Fig. 1 is a device sketch map of the present invention.
The specific embodiment
The present invention is further described below in conjunction with drawings and Examples.
A kind of tissue micro tomography image and spectrographic device of obtaining, is characterized in that it comprises by shown in Figure 1:
One white light source 1 is used to provide illumination light;
One collimation lens 2 and a coupled lens 4 is used to adjust illuminating bundle and signal beams,
The one optical fiber confocal system that connects and composes by single-mode fiber 5 and the probe that constitutes by scanning means 6 and imaging lens group 7, one scan device 6 places between the imaging lens group 7 of single-mode fiber 5 and probe;
Beam splitter 3 and beam splitter 12 realize that the backscatter signal light that the optical fiber confocal system receives on the tissue inner fiber end face conjugate point separates with illumination light, and the separation of flashlight;
One photomultiplier tube detectors that is made of collecting lens 13, pin hole 14 and photomultiplier tube 15 is used to receive the resultant signal light intensity;
A ccd detector that is made of transmission grating 11, collecting lens 10 and line array CCD 9 is used to receive the signal light intensity of each wave band correspondence after transmission grating 11 beam split;
One computer 8 is used to handle signal, demonstration tissue figure information and the spectral information that is obtained by detector and sends the scan control instruction.
The probe of described optical fiber confocal system is drawn together scanning means 6 and imaging lens group 7, and described scanning means is the three-dimensional scanner of being made up of the axial focussing mechanism of mini optical fibre scanning device or micro electronmechanical optical beam scanner and gas or liquid.
The concrete steps that a kind of using method of obtaining tissue micro tomography image and spectrographic device is implemented are:
Step 1: white illumination light imports the optical fiber confocal system of single-mode fiber and probe formation: in Fig. 1, the illumination light that white light source 1 sends enters the optical fiber confocal system through collimating lens 2 and coupled lens 4, adopt collimating lens 2 to help the coupling and the control of light, beam splitter 3 can adopt thin film beam splitter, cube splitter or plate beam splitter, and illuminating bundle is separated with signal beams.Coupled lens 4 will guarantee high coupling efficiency, designs according to single-mode fiber 5 numerical apertures, geometric properties and incident beam diameter.For example: single-mode fiber 5 numerical apertures are 0.1, core diameter is 8 microns, the incident beam diameter is 4 millimeters, then the entrance pupil diameter of coupled lens 4 is 4 millimeters, numerical aperture is 0.1, and the diameter of Aili spot is operated in the diffraction limit scope about 8 microns, achromatism belongs to infinity conjugate distance imaging system.Simultaneously, should place matching fluid between coupled lens 4 and single-mode fiber 5, fiber end face is done polishing, reduces the reflection of fiber end face.
Step 2: the white light through the optical fiber confocal system is assembled on 0-250 μ m degree of depth in tissue, and according to the burnt micro-imaging principle of optical fiber copolymerization, the optical fiber confocal system is the backscatter signal light on the tissue receiving inner fiber end face conjugate point only:
The optical fiber confocal system makes illumination light make 3-D scanning in tissue and obtains backscatter signal light on the scanning element, in order to realize assembling the scanning of luminous point in sample, probe 7 can utilize following sweeping scheme: the axial focussing mechanism of mini optical fibre scanning device or micro electronmechanical optical beam scanner bound gas or liquid.If be used for intravital inspection, also must limit the external diameter and the length of probe according to the biopsy channel size of medical endoscope.The design of probe imaging lens group will be satisfied single-mode fiber co-focusing imaging principle.
In the reflection type optical fiber confocal system, lateral resolution r
tWith axial resolution r
aCan be expressed as:
N is the refractive index of object space in the formula, and nsin α pops one's head in collecting lens in the object space effective numerical aperture, and A is optical fiber-light collecting lens parameter, and
A＝(2πa
0r
0/λd
1)
2
R wherein
0Be optical fiber mode fields radius, a
0/ d
1Effective numerical aperture for the probe light collecting lens.
With described single-mode fiber 5,7 battery of lens of then popping one's head in are made as 0.1 in the effective numerical aperture of optical fiber end in the step 2, design according to resolution requirement in the effective numerical aperture of tissue end, near optical fiber end be oil immersion and be water logging near the tissue end, anaberration.
Step 3: above-mentioned by the optical fiber confocal system receive organize backscatter signal light on the inner fiber end face conjugate point by optical fiber and beam splitter after enter photomultiplier tube detectors and ccd detector respectively, photomultiplier tube receives the resultant signal light intensity, and line array CCD receives the signal light intensity of each wave band correspondence after transmission grating or balzed grating, beam split:
This is to utilize light splitting technology to make two detectors receive the signal light intensity sequence of resultant signal light intensity sequence and each wavelength correspondence respectively, beam splitter 3 will separate by the backscatter signal light that single-mode fiber 5 transmits with beam splitter 12, one the tunnel through lens 13 assemble and pin hole 14 filtering after by photomultiplier tube 15 receptions, guarantee that photomultiplier tube 15 only accepts the overall strength of scanning element place flashlight; Another road is after transmission grating 11 beam split, and the resultant signal light intensity resolves into the signal light intensity of each wavelength correspondence, and the different pixels point that the optical signals of these different wave length correspondences is in the line array CCD 9 on lens 10 focal planes receives.
Step 4: above-mentioned flashlight is imported Computer Processing after opto-electronic conversion, amplification and collection, and the image of computer display organization and spectrum also send the scan control instruction:
This step comprises data acquisition, processing and reconstruct, image and spectrographic demonstration, the sending of control signal.The signal that photomultiplier tube receives is imported computer after amplification, filtering and collection, utilize program restructuring to go out the three-dimensional chromatography micro-image of tissue, can Real Time Observation histiocyte and nuclear geometric shape; The spectral signal that line array CCD 9 receives shows or storage after also input computer 8 is handled after amplification, filtering and collection, obtains organizing the spectrum of biochemical composition; Simultaneously, computer sends scan control and instructs to scanning probe device 6.
The present invention is after obtaining tissue micro tomography image and spectrum, utilize image and spectrum to come the auxiliary judgment tissue whether early stage canceration takes place, the i.e. cell of the normal structure obtained under the same conditions of contrast and the visible light of karyomorphism and biochemical composition detect to organize whether early stage canceration takes place by cell and nuclear metamorphosis and spectrum change.
Claims (3)
1, a kind of tissue micro tomography image and spectrographic device of obtaining is characterized in that it comprises:
One white light source is used to provide illumination light;
One collimation lens and a coupled lens is used to adjust illuminating bundle and signal beams;
One by single-mode fiber and the optical fiber confocal system of popping one's head in and constituting;
A beam splitter, the backscatter signal light on the tissue inner fiber end face conjugate point that realization optical fiber confocal system receives separates with illumination light;
A beam splitter is realized the separation of flashlight;
One photomultiplier tube detectors that is made of collecting lens, pin hole and photomultiplier tube is used to receive the resultant signal light intensity;
A ccd detector that is made of transmission grating or balzed grating,, collecting lens and line array CCD is used to receive the signal light intensity of each wave band correspondence after transmission grating or the balzed grating, beam split;
One computer is used to handle signal, demonstration tissue tomographic map information and the spectral information that is obtained by detector and sends the scan control instruction.
2, a kind of tissue micro tomography image and spectrographic device of obtaining according to claim 1, it is characterized in that, the probe of described optical fiber confocal system comprises scanning means and imaging lens group, and described scanning means is the three-dimensional scanner of being made up of the axial focussing mechanism of mini optical fibre scanning device or micro electronmechanical optical beam scanner and gas or liquid.
3, a kind of using method of obtaining tissue micro tomography image and spectrographic device is characterized in that, method step is:
1) white illumination light is imported the optical fiber confocal system that single-mode fiber and probe constitute;
2) assemble on 0-250 μ m degree of depth in tissue through the white light of optical fiber confocal system, according to the burnt micro-imaging principle of optical fiber copolymerization, the optical fiber confocal system is the backscatter signal light on the tissue receiving inner fiber end face conjugate point only;
3) above-mentioned by the optical fiber confocal system receive organize backscatter signal light on the inner fiber end face conjugate point by optical fiber and beam splitter after enter photomultiplier tube detectors and ccd detector respectively, photomultiplier tube receives the resultant signal light intensity, and line array CCD receives the signal light intensity of each wave band correspondence after transmission grating or balzed grating, beam split;
4) described flashlight is imported Computer Processing after opto-electronic conversion, amplification and collection, and the image of computer display organization and spectrum also send the scan control instruction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200610118100XA CN100464692C (en) | 2006-11-09 | 2006-11-09 | Method and apparatus for obtaining tissue micro tomography image and spectrum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200610118100XA CN100464692C (en) | 2006-11-09 | 2006-11-09 | Method and apparatus for obtaining tissue micro tomography image and spectrum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1961819A CN1961819A (en) | 2007-05-16 |
| CN100464692C true CN100464692C (en) | 2009-03-04 |
Family
ID=38081171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200610118100XA Expired - Fee Related CN100464692C (en) | 2006-11-09 | 2006-11-09 | Method and apparatus for obtaining tissue micro tomography image and spectrum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100464692C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101158640B (en) * | 2007-11-06 | 2010-06-09 | 山东大学 | Device and method for rapid measuring coherent backscattering by linear array CCD |
| CN105067617A (en) * | 2015-07-21 | 2015-11-18 | 上海理工大学 | Cell recognition apparatus and method based on phase contrast image and confocal scattering microspectrum |
| CN105424601B (en) * | 2015-12-22 | 2018-02-16 | 广东欧谱曼迪科技有限公司 | A kind of hand-held is copolymerized burnt skin microscopic method and device |
| CN108303421B (en) * | 2018-01-02 | 2020-02-11 | 清华大学 | Three-dimensional high-speed wide-field tomography method and device |
| CN111007054A (en) * | 2018-10-08 | 2020-04-14 | 天津大学 | Raman spectrum detection device with white light imaging function |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6002480A (en) * | 1997-06-02 | 1999-12-14 | Izatt; Joseph A. | Depth-resolved spectroscopic optical coherence tomography |
| CN2401883Y (en) * | 1999-12-28 | 2000-10-18 | 中国科学院西安光学精密机械研究所 | High flux tomographic spectrum device |
| CN2457581Y (en) * | 2000-12-28 | 2001-10-31 | 中国科学院西安光学精密机械研究所 | Raster type chromatographic imaging spectrum device |
| CN2651731Y (en) * | 2003-11-13 | 2004-10-27 | 中国科学院西安光学精密机械研究所 | Rotary raster chromatography imaging spectrograph |
| WO2006076810A1 (en) * | 2005-01-21 | 2006-07-27 | Perceptronix Medical Inc. | Method And Apparatus For Measuring Cancerous Changes From Reflectance Spectral Measurements Obtained During Endoscopic Imaging |
-
2006
- 2006-11-09 CN CNB200610118100XA patent/CN100464692C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6002480A (en) * | 1997-06-02 | 1999-12-14 | Izatt; Joseph A. | Depth-resolved spectroscopic optical coherence tomography |
| CN2401883Y (en) * | 1999-12-28 | 2000-10-18 | 中国科学院西安光学精密机械研究所 | High flux tomographic spectrum device |
| CN2457581Y (en) * | 2000-12-28 | 2001-10-31 | 中国科学院西安光学精密机械研究所 | Raster type chromatographic imaging spectrum device |
| CN2651731Y (en) * | 2003-11-13 | 2004-10-27 | 中国科学院西安光学精密机械研究所 | Rotary raster chromatography imaging spectrograph |
| WO2006076810A1 (en) * | 2005-01-21 | 2006-07-27 | Perceptronix Medical Inc. | Method And Apparatus For Measuring Cancerous Changes From Reflectance Spectral Measurements Obtained During Endoscopic Imaging |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1961819A (en) | 2007-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101909509B (en) | Multi-path, multi-magnification, non-confocal fluorescence emission endoscopy apparatus and methods | |
| Chen et al. | Optical and digital microscopic imaging techniques and applications in pathology | |
| CN101904737B (en) | Living body fluorescent endoscopic spectrum imaging device | |
| CN101375786B (en) | Fluorescence endoscopic imaging method and device | |
| CN100464692C (en) | Method and apparatus for obtaining tissue micro tomography image and spectrum | |
| CN1341209A (en) | Imaging of tissue using polarized light | |
| CN112666698B (en) | Dispersive super-surface-based fiber bundle multi-azimuth three-dimensional confocal imaging device and method | |
| CN103163111A (en) | Early stage cervical carcinoma detection system integrating fluorescent mesoscope imaging and optical coherence tomography (OCT) | |
| CN102389288B (en) | Confocal laser micro-endoscope | |
| CN103940796A (en) | Novel multi-angle and multi-mode quick switching circular optical illumination microscopic imaging system | |
| CN102818768A (en) | Multifunctional biomedical microscope | |
| CN109839732A (en) | A kind of cage construction laser scanning co-focusing micro imaging system and method | |
| CN110464309B (en) | Cross-scale fluorescence endoscopic imaging system | |
| CN1167946C (en) | Spatial multichannel fiber coupler with laser induced synchronous fluorescence detection | |
| CN111568377B (en) | Optical scanning probe for gynecological examination and working method thereof | |
| US7336990B2 (en) | Equipment for subsurface autofluorescence spectroscopy | |
| CN110353609A (en) | A kind of light field 3D confocal endoscope having three-dimensional imaging ability | |
| CN104849252A (en) | Three-color fluorescent microscopic imaging system | |
| CN102488494A (en) | Totally internal reflection type photo-acoustic microscopic imaging system and method | |
| CN101361651B (en) | Fluorescent molecule fault imaging device | |
| US8700135B2 (en) | Optical fiber array probe imaging system integrated with endoscope | |
| CN109085119A (en) | A kind of the copolymerization coke 3-D imaging system and implementation method of the detection of Raman tomographic spectroscopy | |
| CN101303315B (en) | Optical fiber common focusing micro spectrum and imaging apparatus of cell analysis | |
| CN101940463B (en) | Fluorescence endoscopic imaging system for living body | |
| CN207636480U (en) | A kind of EO-1 hyperion microscopic imaging apparatus can be used for insertion type diagnosing tumor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090304 Termination date: 20111109 |