CN100478676C - Formaldehyde repid test kit and its use - Google Patents
Formaldehyde repid test kit and its use Download PDFInfo
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- CN100478676C CN100478676C CNB2005100163902A CN200510016390A CN100478676C CN 100478676 C CN100478676 C CN 100478676C CN B2005100163902 A CNB2005100163902 A CN B2005100163902A CN 200510016390 A CN200510016390 A CN 200510016390A CN 100478676 C CN100478676 C CN 100478676C
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Abstract
Without need of additional instrument, the kit is capable of mensurating concentration range of formaldehyde contained in article rapidly; and minimum limit of detection is 0.8mg/Kg. The disclosed kit includes injector of active carbon, reaction liquid, test tube, and standard color grade. The reaction liquid is prepared from acetylacetone, glacial acetic acid, and ammonium acetate according to mol ratio per hundred milliliter 39:105:4000. The standard color grade is a color card in series of light yellow. The method includes steps: adding the reaction liquid into supernatant solution from dipped specimen to be tested; after 6 min, comparison is carried out between developed color and standard color grade; determining content range of formaldehyde. Features are: simple operation, quick and sensitive and widely available to test in site.
Description
Technical field
The invention belongs to measure and use kit, relate to a kind of kit and assay method thereof of fast measuring formaldehyde in food content, especially at the scene fast measuring.
Background technology
Formaldehyde, molecular formula HCHO, colourless gas.Fusing point-92 ℃, boiling point-21 ℃, relative density 0.815 (20/4 ℃).The strong and stimulating smell is arranged.Soluble in water, be dissolved in organic solvents such as ethanol, ether.The aqueous solution of concentration of formaldehyde between 37%-40% is commonly referred to formalin.Formaldehyde is a kind of protoplasm poisonous substance that has than the destruction biological cell protein of high toxicity, can combine with the amino of protein, and protein denaturation is solidified, and upsets cellular metabolism.Formaldehyde is carcinogenic and teratogen simultaneously, is one of potential strong mutagen.The formaldehyde of long-term contact low dosage can cause gene mutation, causes cancer.In addition, formaldehyde mainly causes damage to people's central nervous system, respiratory system digestive system and internal system.Clinical symptoms shows as forfeiture appetite, n and V even causes death.This shows that formaldehyde is very big to human health damage, so forbid adding formaldehyde in various countries, the world regulation food.In recent years, many food processing persons because economic interests are ordered about, added formalin in food processing, and its fundamental purpose is to play the corrosion-resistant effect.Thereby study a kind of convenient and swiftly, and being beneficial to operation, cheap experimental assembly helps the on-site measurement of ruck, also can be quality inspection organization and judges as the basis and use.
The existing method of measuring formaldehyde of China mainly contains phenol reagent process, chromotropic acid colourimetry, dinitro phenyl hydrazine method, silver mirror method, high performance liquid chromatography etc.Wherein high performance liquid chromatography and chromotropic acid colourimetry all need instrumental analysis, are unfavorable for on-site measurement, and acetaldehyde has interference to the chromotropic acid colourimetry simultaneously.Dinitro phenyl hydrazine method is applicable to the substance-measuring that contains carbonyl, all can be influential so have the material of carbonyl to this method, cause misjudgment easily.The required reagent costliness of phenol reagent process is unfavorable for promoting.The sensitivity of silver mirror method is too low, and needs heating in the testing process, also is unfavorable for fast measuring.Have following problem in the method for existing colorimetric method for determining formaldehyde: the method that has need be equipped with spectrophotometer; The aqueous solution of the method that has after sample soaks often has color, and can have certain influence to the judgement of color this moment after adding developer; The method that has need add thermal distillation to sample, when the method that has is reacted with detection liquid at sample, needs the water-bath heating.Above-mentioned heating process had not only wasted energy but also had not met the requirement of fast detecting.The sensitivity of existing method for quick is not high, is about 1.0mg/Kg.In a word, there is not suitable on-the-spot detection to require at present as yet, is limited to the fast measuring kit of the mensuration formaldehyde in food of 0.8mg/Kg under detecting.
Summary of the invention
The objective of the invention is in order to solve the deficiency that the above-mentioned background technology exists, a kind of kit and detection method thereof that need not to heat, have simple to operate, rapid sensitive, adaptation execute-in-place characteristics, fast detecting formaldehyde in food is provided.
Technical scheme:
Formaldehyde repid test kit, the box that it is made up of the syringe that activated charcoal is housed, reactant liquor, test tube and standard color comparison card; Wherein reactant liquor is formulated by adding 15.4g ammonium acetate, 0.2mL diacetone and 0.3mL glacial acetic acid in per hundred ml waters.Standard color comparison card is the colour atla of faint yellow series.This card minimum detectability is 0.8mg/Kg.
Need to prove:
The syringe specification is selected the 5mL syringe for use in the kit, and quantity is that 5-10 props up, and the activated charcoal that is equipped with in the syringe is an activated carbon powder, and its quality is each required amounts of activated carbon of measuring, and is 0.5-2.0g.
The faint yellow series of standard color comparison card is for being divided into 8-10 class from shallow to deep; And the concentration of formaldehyde value that the corresponding respectively mark of different colours is measured is 0.8mg/Kg to 20.0mg/Kg.
The application of formaldehyde repid test kit is used for the formaldehyde in food fast Determination.Its concrete assay method is: be soaked in 10-20min in the 50mL distilled water after sample 10-20g is pulverized, it is stand-by to get supernatant, if the aqueous solution after soaking has the color of sample itself, the syringe of activated charcoal the supernatant process of soaking can be equipped with, with the background colour removal, avoid influencing result of determination, get filtrate after supernatant 5mL to be measured or the decolouring then and join in the 2mL reactant liquor that (wherein reactant liquor is by adding the 15.4g ammonium acetate in per hundred ml waters, 0.2mL diacetone and 0.3mL glacial acetic acid are formulated), observe whether change color is arranged in the test tube after the 6min, if change color is arranged, with color and the comparison of the respective color on the standard color comparison card that forms, select the close standard color comparison card value of color, be the concentration range that sample contains formaldehyde.
The adsorbability of activated charcoal PARA FORMALDEHYDE PRILLS(91,95) is extremely faint, does not exert an influence for this experiment.Available following evidence, specific practice is: the formalin of getting 0.8mg/Kg concentration is through having the syringe of activated charcoal, get the reaction of 5mL filtrate and 2mL reactant liquor, get the direct and 2mL reactant liquor reaction of (0.8mg/Kg) formalin 5mL of same concentrations simultaneously, it is identical to produce color.As shown in Figure 1 and Figure 2, color is identical, can't distinguish.
Beneficial effect:
Formaldehyde repid test kit provided by the invention, having changed the detection of PARA FORMALDEHYDE PRILLS(91,95) in the past needs instrument, wastes time and energy, and is not suitable for the deficiency of on-site measurement.This new detection method has not need heating, rapid sensitive, cheap, be beneficial to universal, be suitable for characteristics such as on-site measurement.The minimum concentration of formaldehyde that detects of the present invention is 0.8mg/Kg.
Description of drawings
Fig. 1: through the result of filtrate after the charcoal absorption and reactant liquor reaction;
Fig. 2: without the result of supernatant after the mistake charcoal absorption and reactant liquor reaction;
Fig. 3: standard color comparison card of the present invention (color is faint yellow serial colour atla).
Embodiment
Embodiment 1:
Formaldehyde repid test kit comprises that 5 of syringes (5mL), 5 in test tube (15*17mm), reactant liquor 20mL (its compound method is: add 15.4g ammonium acetate, 0.2mL diacetone, 0.3mL glacial acetic acid in per hundred ml waters), 5 in test tube, standard color comparison card that the 1g activated charcoal is housed are (faint yellow for being divided into 8 class compositions from shallow to deep; The concentration of formaldehyde that corresponding respectively mark is measured is followed successively by 0.8mg/Kg, 1.0mg/Kg, 2.0mg/Kg, 5.0mg/Kg, 7.0mg/Kg, 9.0mg/Kg, 15.0mg/Kg and 20.0mg/Kg), (the standard color comparison card color is faint yellow series as shown in Figure 3, its color is determined the mask method with the content of formaldehyde value, be to get configuration 0.8mg/Kg respectively, 1.0mg/Kg, 2.0mg/Kg, 5.0mg/Kg, 7.0mg/Kg, 9.0mg/Kg15.0mg/Kg and 20.0mg/Kg standard solution 5mL respectively joins in the 2mL reactant liquor, observe change color through 6min, liquid color is deepened successively with the increase of concentration of standard solution in the test tube, these colors are the Yellow series of standard color comparison card, and from shallow to deep the pairing content of formaldehyde value of color are labeled as 0.8mg/Kg under the standard color comparison card colour band, 1.0mg/Kg, 2.0mg/Kg, 5.0mg/Kg, 7.0mg/Kg, 9.0mg/Kg, 15.0mg/Kg and 20.0mg/Kg.That color is the lightest is 0.8mg/Kg, and the dark more concentration of color is big more.)
Measure Application Example 1:
Use the kit of embodiment 1, detect.Sample water sent out be soaked in 15min in the beaker that fills 50mL distilled water after squid 10g pulverizes, then the 5mL supernatant is joined in the test tube that the 2mL reactant liquor is housed in advance, 6min has color to produce, at last with the color and the comparison of the respective color on the standard color comparison card that form, dark colour is close on its color and the standard color comparison card, according to this color colorimetric calorific power is 20mg/Kg, determine sample water send out concentration range that squid contains formaldehyde be 20mg/Kg or and more than.
Embodiment 2:
Formaldehyde repid test kit comprises that 10 in 10 of syringes (5mL), reactant liquor 50mL, test tube, standard color comparison card that the 0.5g activated charcoal is housed are (faint yellow for being divided into 10 class from shallow to deep; Its reactant liquor preparation and mask method are with embodiment 1).Marking concentration of formaldehyde along with yellow is corresponding respectively from shallow to deep on the standard color comparison card is 0.8mg/Kg, 1.0mg/Kg, 2.0mg/Kg, 3.0mg/Kg, 5.0mg/Kg, 7.0mg/Kg, 9.0mg/Kg, 10.0mg/Kg, 15.0mg/Kg, 20.0mg/Kg.
Measure Application Example 2:
Use the kit of embodiment 2, detect.Sample pestered be soaked in 20min in the beaker that fills 50mL distilled water after 15g pulverizes, the color that sample itself is arranged because of the aqueous solution after soaking, soak solution is crossed the activated charcoal syringe, make the background colour removal, judge in order to avoid influence the result, then the filtrate after the 5mL decolouring is joined in the 2mL reactant liquor, 6min has color to produce, low haze is close on its color and the standard color comparison card, according to this color colorimetric calorific power is 0.8mg/Kg, determine sample water send out concentration range that squid contains formaldehyde be 0.8mg/Kg or and below.
Embodiment 3:
Formaldehyde repid test kit comprises that 8 in 8 of syringes (5mL), reactant liquor 20mL, test tube, standard color comparison card that the 1.5g activated charcoal is housed are (faint yellow for being divided into 8 class from shallow to deep; Its reactant liquor preparation and mask method are with embodiment 1).Marking concentration of formaldehyde along with yellow is corresponding respectively from shallow to deep on the standard color comparison card is 0.8mg/Kg, 1.0mg/Kg, 2.0mg/Kg, 5.0mg/Kg, 7.0mg/Kg, 9.0mg/Kg, 15.0mg/Kg, 20.0mg/Kg.
Measure Application Example 3:
Use the kit of embodiment 3, detect.Sample pestered be soaked in 15min in the beaker that fills 50mL distilled water after 20g pulverizes, aqueous solution after the immersion has the color of sample itself, soak solution is crossed the activated charcoal syringe, make the background colour removal, judge in order to avoid influence the result, then filtrate the joining after the 5mL decolouring held in the test tube of 2mL reactant liquor, 6min has color to produce, the 7mg/Kg-9mg/Kg color is close on its color and the standard color comparison card, determines that sample water sends out the concentration range 7mg/Kg-9mg/Kg that squid contains formaldehyde.
Claims (4)
1. formaldehyde repid test kit is characterized in that the box of being made up of the syringe that activated charcoal is housed, reactant liquor, test tube and standard color comparison card; Wherein reactant liquor is formulated by adding 15.4g ammonium acetate, 0.2mL diacetone and 0.3mL glacial acetic acid in per hundred ml waters, and standard color comparison card is the colour atla of faint yellow series, and this card minimum detectability is 0.8mg/Kg.
2. according to the described formaldehyde repid test kit of claim 1, it is characterized in that the syringe specification is the 5mL syringe, quantity is that 5-10 props up, and the activated charcoal that is equipped with in the syringe is an activated carbon powder, and quality is each required amounts of activated carbon of measuring, and is 0.5-2.0g.
3. according to the described formaldehyde repid test kit of claim 1, it is characterized in that the faint yellow of standard color comparison card for being divided into 8-10 class from shallow to deep; And the concentration of formaldehyde that the corresponding respectively mark of different colours is measured is 0.8mg/Kg to 20.0mg/Kg.
4. the application of formaldehyde repid test kit is characterized in that being used for the formaldehyde in food fast Determination; Concrete assay method is to be soaked in 10-20min in the 50mL distilled water after sample 10-20g is pulverized, and it is stand-by to get supernatant; If the aqueous solution after soaking has the color of sample itself, the syringe of activated charcoal the supernatant process of soaking is equipped with, with the background colour removal, avoid influencing result of determination, get then stand-by supernatant 5mL or the decolouring after filtrate join in the 2mL reactant liquor, wherein reactant liquor is by adding the 15.4g ammonium acetate in per hundred ml waters, 0.2mL diacetone and 0.3mL glacial acetic acid are formulated, observe after the 6min whether change color is arranged, if change color is arranged, with color and the comparison of the respective color on the standard color comparison card that forms, select the close standard color comparison card value of color, be the concentration range that sample contains formaldehyde.
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| CN100478676C true CN100478676C (en) | 2009-04-15 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101623582B (en) * | 2009-07-30 | 2011-09-28 | 上海交通大学 | Device for indicating and removing formaldehyde |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103196902A (en) * | 2012-01-04 | 2013-07-10 | 窦荣艳 | Air formaldehyde self-detecting box |
| CN102590197B (en) * | 2012-02-01 | 2017-03-08 | 美的集团股份有限公司 | A kind of formaldehyde testing equipment |
| CN103185716A (en) * | 2012-12-21 | 2013-07-03 | 深圳市谱尼测试科技有限公司 | Method for rapidly screening free formaldehyde in cosmetics onsite |
| US10055837B2 (en) | 2014-11-04 | 2018-08-21 | Samsung Electronics Co., Ltd. | Method of and apparatus for measuring biometric information |
| CN106525839B (en) * | 2016-12-16 | 2019-02-22 | 上海烟草集团有限责任公司 | A method of content of formaldehyde in paper wrapper is quickly detected based on film enrichment colorimetric |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101623582B (en) * | 2009-07-30 | 2011-09-28 | 上海交通大学 | Device for indicating and removing formaldehyde |
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