CN100529752C - Analyzing method and apparatus employing magnetic material - Google Patents
Analyzing method and apparatus employing magnetic material Download PDFInfo
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- CN100529752C CN100529752C CNB2005100813173A CN200510081317A CN100529752C CN 100529752 C CN100529752 C CN 100529752C CN B2005100813173 A CNB2005100813173 A CN B2005100813173A CN 200510081317 A CN200510081317 A CN 200510081317A CN 100529752 C CN100529752 C CN 100529752C
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Abstract
The invention relates to an analysis method which combines magnetic material and acoustic sensitive element, and relative device, wherein it uses the magnetic material decorated with specific recognize molecule to catch the tested material of sample, and provides external magnetic field to guide the magnetic material to the sensitive field of acoustic sense element; then converting the acoustic effect caused by the magnetic material and tested material on the surface of sense field into the content of tested material in the analyzed sample.
Description
Technical field
The invention relates to a kind of analytical approach and device that uses magnetic material and acoustics sensing component, further, the invention relates to a kind of analytical approach and device in conjunction with magnetic material and microbalance sensor.
Background technology
Test substance has two main policies in the general survey sample, the one, via solvent extraction, concentrate, separations step purifying test substances such as precipitation or chromatography carry out quantitatively again, the one, utilize the content of predetermined substance in the direct measurement of the selectivity reaction complex sample.Elder generation's purifying carries out the destruction that quantitative methods is often followed the biological substance activity again, and if step is too numerous and diverse tediously long generation of easily following error.Measure towards utilizing selectivity to react so the development of present analytical technology is many, and biology sensor just meets this technological development demand.
General biology sensor is made up of identification molecule, transducer and signal processor, wherein the identification molecule is the induction region surface that is positioned at transducer, the purposes of identification molecule is to contact with determinand to produce reaction, so the identification molecule need possess the specificity to determinand, increasing Selectivity of Sensor, general identification molecule make a living soma, microorganism, born of the same parents' device, cell receptor, ferment, antigen or antibody.
The function of transducer is with the change of identification molecule and the reacted physical quantity of test substance or chemistry amount, converts electronic signal to, physical quantity or chemistry amount comprise optics, qualitative perception should, thermochemistry, galvanochemistry and magnetic induction etc.For example: when using QCM (Quartz Crystal Microbalance) in the acoustics sensing component as transducer, coat identification molecule on the quartz surfaces to the absorption of test substance, the quality that can cause the quartz crystal surface changes, and causes the frequency shift of quartz crystal, just can measure the concentration of test substance indirectly.Yet method with the induction region finishing identification molecule of acoustics sensing component, also will limit to the test substance kind that this acoustics sensing component can detect, for more efficient and by limitation do not utilized the high sensitivity of acoustics sensing component, the magnetic material that is modified with the identification molecule just is directed in the exploitation of correlation technique.
(US6 in a prior art, 294,342) be to use one modified and magnetic reagent and that have an identification test substance ability moves stationary phase reagent (mobilesolid phase reagent), by by combining between two reagent and test substance, under the effect of externally-applied magnetic field to the effect of measuring equipment.Yet the magnetic reagent (magnetically reagent) that this technology is used needs just to be enough to measuring equipment is produced the effect that can be measured with mobile stationary phase reagent collocation use, this effect is mainly that suction and repulsion between magnet and the magnetic bead changes and the weighing amount that causes changes, yet μ g grade is only arrived in the sensitivity of this method, and causes test substance to separate out or precipitate easily.
US5,660,990 is after utilizing one to catch reagent seizure desire analyte, should catch reagent again combines with signal generation reagent, the technology that the signal that produces through detection signal generation reagent place detects, therefore, its embodiment is mainly based on optical detection.Yet the use of additional agents causes the increase of step and the generation that all can cause detecting error in conjunction with the restriction of condition.In addition at US6,342, in 396, it is the fixing acceptor of catching on a trap setting surface, but again by catch the particle of an identification determinand by magnetic field, the method is except involving loaded down with trivial details surface modification technology, and specific seizure acceptor will cause trap setting to use also on the trap setting can only perhaps need to change the trap setting surface and just can carry out the analysis that other desires analyte at particular test; Embodiment on it detects is then observed with atomic force microscope, does not therefore have the value that is widely used.
So one sensitiveer, simple and do not need extra demarcation signal to produce the biology sensor measuring equipment and the method for reagent, is the exploitation direction in present technique field.
Summary of the invention
Because the disappearance of prior art, the present invention provides a kind of analytical approach and device that uses magnetic material, it is to utilize modified magnetic material to catch the test substance of desiring in the analytic sample, and providing externally-applied magnetic field that magnetic material is drawn to the induction region of acoustics sensing component, the effect that the induction region surface is produced is scaled the content of desiring test substance in the analytic sample then.
The method that the purpose of this invention is to provide test substance in a kind of analytic sample, its step comprises: a magnetic material a) is provided, and described magnetic material surface has the identification molecule that can catch test substance in the sample; B) described magnetic material is added in the sample, make the identification molecule on described magnetic material surface catch test substance in the sample; C) utilize a magnetic field that described magnetic material is directed to the induction region surface of an acoustics sensing component, make described induction region surface produce acoustic effect; And d) described acoustic effect is scaled the content of test substance in the sample.
Another object of the present invention provides the device of test substance in a kind of analytic sample, is to comprise: a reactive tank, this reactive tank have a space as the sample importing; One magnetic material, this magnetic material surface have the identification molecule that can catch test substance in the described sample; One magnetic field producer is in order to provide the magnetic material in the described reactive tank one externally-applied magnetic field; And an acoustics sensing component, this acoustics sensing component has an induction region that is exposed in the described reactive tank; Wherein said device is to utilize externally-applied magnetic field that magnetic field producer provides that the test substance of described magnetic material and seizure thereof is focused to the induction region surface of described acoustics sensing component, makes the induction region surface produce acoustic effect and obtains test substance content in the sample to convert.
Described device of the present invention can further comprise an optics sensing component, in order to detect described magnetic material.
The device of test substance in the analytic sample of the present invention, wherein an embodiment of this reactive tank is a container, with so that sample static state be stored in this container.Another embodiment of described reactive tank is a pipeline, with so that sample dynamically circulate in this pipeline.
For magnetic separation technique, numerous and diverse steps such as the present invention need not use solvent extraction, concentrates, precipitation or chromatography, can reach the separation of material with quantitatively; Use for biochemical sensor, remove the degree of difficulty that to exempt the finishing of acoustics sensing component, but the also means of sampling pre-separation and purifying have reduced non-desire analyte and separated and quantitative interference.
Description of drawings
Fig. 1 is an analytical equipment synoptic diagram of the present invention, and its magnetic field producer does not provide externally-applied magnetic field, and reactive tank is the container aspect;
Fig. 2 is an analytical equipment synoptic diagram of the present invention, and its magnetic field producer provides an externally-applied magnetic field, and reactive tank is the container aspect;
Fig. 3 is an analytical equipment synoptic diagram of the present invention, and its magnetic field producer provides an externally-applied magnetic field, and reactive tank is the pipeline aspect;
Fig. 4 is an analytical equipment synoptic diagram of the present invention, and its reactive tank is the container aspect, and has a magnetic material collecting zone;
Fig. 5 is an analytical equipment synoptic diagram of the present invention, and its reactive tank is the pipeline aspect, and has a magnetic material collecting zone;
Fig. 6 A and Fig. 6 B are analytical equipment synoptic diagram of the present invention, and its reactive tank is the pipeline aspect, and have fluid mobile route in the variable valve may command pipeline;
Fig. 7 is the effect trend figure that externally-applied magnetic field magnetic material produces the acoustics sensing component;
Fig. 8 is the effect trend figure that different magnetic materials produce the acoustics sensing component under the externally-applied magnetic field;
Fig. 9 is under the externally-applied magnetic field of getting rid of non-specific adsorption, and different magnetic materials are to the effect trend figure of acoustics sensing component generation;
Figure 10 catches the effect trend figure that under externally-applied magnetic field the acoustics sensing component is produced behind the desire analyte for magnetic material.
[the primary clustering symbol is to as directed]
1 reactive tank
2 magnetic field producers
3 acoustics sensing components
31 induction regions
4 signal processors
5 magnetic materials
51 identification molecules
6 testing samples
61 desire analyte
62 non-desire analytes
7 magnetic material collecting zones
8 variable valve
81,82 paths
91,92 magnetic field producers
100 analytical equipments of the present invention
Embodiment
The method of utilizing test substance in the magnetic material analytic sample of the present invention, it is that a magnetic material at first is provided, magnetic material is meant the material that can utilize the externally-applied magnetic field guiding and move, and comprises magnetic particle, magnetic nano-particle or super paramagnetic nano particle.For catching the test substance in the sample, the magnetic material surface is to have the identification molecule, identification molecule inclusion compound, antigen, antibody, acceptor, part, ferment, protein, victory peptide or nucleic acid, and it is to select for use according to the characteristic of test substance.The magnetic material that will have the identification molecule adds in the sample, after making the identification molecule on described magnetic material surface catch test substance in the sample, promptly utilize a magnetic field that described magnetic material is directed to the induction region surface of an acoustics sensing component, make described induction region surface produce acoustic effect.The alleged acoustics sensing component (acoustic sensor) of the present invention comprises, but be not limited to QCM (Quartz Crystal Microbalance), surface acoustic wave component, bent flat Lamb wave assembly, dull and stereotyped ripple assembly or micro-cantilever, when magnetic material contacts the induction region of acoustics sensing component with the compound of test substance combination, deformation quantity can take place and change in the induction region surface, the resonant frequency shift quantitative changeization, acoustic effects such as acoustic signals variation, the test substance of variable concentrations causes acoustic effect in various degree, so can be scaled the content of test substance in the sample by the degree by acoustic effect.
Because the magnetic material of part or further be doped with the magnetic material of photographic composition itself, the radiation with different optical wavelength or the absorption characteristic that can make, visible light for example, the radiation of infrared light or ultraviolet light or absorption, fluorescent radiation, phosphorescence radiation or cold light radiation, and when these magnetic materials capture the test substance of variable concentrations, it can produce in various degree light emission or light absorption, so except utilizing acoustics sensing component detection of acoustic effect, also can further utilize the optics sensing component to detect the variation of light emission or light absorption, even can further modify signaling molecule on the surface of magnetic material, strengthen the characteristic of light emission or light absorption, the effect when test substance is combined with magnetic material is more obvious.In addition, by by the optical characteristics between different magnetic materials,, then can be applicable to sample diversity analysis or detection if arrange in pairs or groups different identification molecular modifications.
An embodiment of test substance device 100 is as shown in Figure 1 in the analytic sample of the present invention, is to comprise reactive tank 1, magnetic field producer 2, acoustics sensing component 3, signal processor 4 and magnetic material 5.Wherein reactive tank 1 is a container, can be in order to testing sample 6 static state be stored in wherein, magnetic material 5 is that mixed distribution is in testing sample 6, magnetic material 5 of the present invention is modified magnetic particle, magnetic nano-particle or super paramagnetic nano particle with identification molecule 51, its size is to can be micron or nanometer size, optionally desires analyte 61 by catching in the testing sample 6; Modify in the identification molecule 51 on magnetic material 5 surfaces and comprise, but be not limited to compound, antigen, antibody, acceptor, part, ferment, protein, victory peptide or nucleic acid, but it is to have the characteristic that analyte 61 is desired in identification.Method in magnetic material 5 finishing identification molecules 51 is known by the present technique field, as: utilize when magnetic material 5 is constituted by iron oxide, the hydroxyl that is had on it can form chemical bonded refractory with alkoxy-silicon compound, utilize on the alkoxy-silicon compound other functional group and identification molecule 51 to form chemical bonded refractories and reach the modification purpose again, the poly-candy (dextran) of grape also can utilize similar reaction to reach the modification purpose in addition.Acoustics sensing component 3 comprises, but be not limited to QCM (Quartz Crystal Microbalance), surface acoustic wave component, bent flat Lamb wave assembly, dull and stereotyped ripple assembly or micro-cantilever, be to use quartz crystal microbalance sensor in the embodiments of the invention, this microbalance sensor has an induction region 31 that is exposed in the reactive tank 1, its be arranged at magnetic field producer 2 when externally-applied magnetic field is provided magnetic material 5 be pulled the zone of gathering, this zone is the bottom that can comprise reactive tank, all around or the top, be preferably the top that is positioned at reactive tank 1, can reduce in the analytic process material of desiring beyond the analyte 61 (being non-desire analyte 62) by this and be adsorbed in induction region 31 because of gravity causes precipitation, and then impact analysis result, and magnetic field producer 2 is for providing the device of reactive tank 1 one externally-applied magnetic fields, comprise, but be not limited to inductive coil or magnet.
With reference to figure 2, when magnetic field producer 2 provides an externally-applied magnetic field in magnetic material 5, magnetic material 5 will be pulled and be focused to induction region 31 surfaces, the number of the desire analyte 61 that caught by magnetic material 5 this moment, induction region 31 surface generations effect in various degree will be made, this effect comprises, but the par that is not limited to magnetic material changes, the buildup effect of magnetic material, viscosity changes, variable density, magnetic force change, temperature variation and charge variation, and the generation meeting of described effect is related 3 generations of acoustics sensing component are comprised, but being not limited to deformation quantity changes, the resonant frequency shift quantitative changeization, acoustic signals changes, signal processor 4 is the degree that analytical acoustics sensing component 3 changes, and then be scaled and desire the content of analyte 61 in testing sample 6, it is a part that can be independent device or be incorporated into acoustics sensing component 3 for a signal processor 4.
Fig. 3 is another embodiment of the present invention, and wherein reactive tank 1 is the pipeline form, and testing sample 6 can dynamically be circulated in the reactive tank 1 to reach successional sample detection pattern.When in being mixed in testing sample 6, flowing through the externally-applied magnetic field that magnetic field producer 2 provides in order to magnetic material 5 that catch to desire analyte 61, be gathered in acoustics sensing component 3 and be exposed to induction region 31 surfaces in the reactive tank 1 being pulled, desire analyte 61 and non-desire analyte 62 in the testing sample 6 can reach separating effect at this moment, the magnetic material 5 that is gathered in induction region 31 can make acoustics sensing component 3 change, and the degree that signal processor 4 can change acoustics sensing component 3 is scaled and desires the content of analyte 61 in testing sample 6.
An embodiment more of the present invention is shown in Fig. 6 A and Fig. 6 B, among Fig. 6 A, the testing sample 6 that contains magnetic material 5 is the magnetic field of a magnetic field producer 91 manufacturings of flowing through earlier, having caught the magnetic material 5 of desiring analyte 61 this moment will be stranded in the magnetic field, contained non-desire analyte 62 then behind the magnetic field of flowing through, moves and does not analyze by the directions of a variable valve 8 backs toward paths 81 in the testing sample 6.
Before variable valve 8 conversions, the action that can earlier the magnetic material 5 that is stranded in the magnetic field be cleaned, by to remove the non-analyte 62 that wherein may influence the subsequent analysis result, adjust variable valve 8 afterwards, make flowing of fluid transfer the direction in path 82 to by path 81, close magnetic field producer 91 this moment, the magnetic material 5 that is stranded in the magnetic field begins to flow because of the disappearance in magnetic field, by variable valve 8 back access paths 82, the influence that this moment, magnetic material 5 was subjected to the magnetic field of magnetic field producer 92 manufacturings is pulled the induction region surface 31 that is focused to acoustics sensing component 3, signal processor 4 just can obtain the signal of acoustics sensing component 3 intensity of variations, and then is scaled the content of desire analyte 61 in testing sample 6.
Following examples are to be used for further understanding advantage of the present invention, are not to be used to limit claim of the present invention.
The effect that embodiment 1. externally-applied magnetic field magnetic particles produce the acoustics sensing component
Present embodiment picks up from synthetic magnetic ferric oxide nano particles (the about 10nm of mean grain size of row; Working concentration: 20 μ g/ml), cooperate fluid-pouring-in (flow-injectionanalysis, FIA; Use flow velocity: 160 μ l/ml) (quartzcrystal microbalance, QCM) system experimentizes QCM (Quartz Crystal Microbalance).The magnetic field source of supply then adopts a High-Field value magnet (the about 100mT of magnetic field intensity that acoustics sensing component surf zone is exposed).Experimental result as shown in Figure 7.
As shown in Figure 7, under the situation that externally-applied magnetic field is not provided, the oscillation frequency of QCM (Quartz Crystal Microbalance) is 13Hz, and when externally-applied magnetic field was provided, shaking increased to 46Hz when frequency.This result is that proof is providing under the externally-applied magnetic field effect, and magnetic particle can be because be gathered in the induction region of QCM (Quartz Crystal Microbalance), causes quartz crystal generation piezoelectric effect and changes oscillation frequency.
The effect that different magnetic particles produce the acoustics sensing component under embodiment 2. externally-applied magnetic fields
The purpose of present embodiment is to assess the feasibility that the present invention is used for biochemistry detection.Experimental provision setting and embodiment 1 are together.Experimental result as shown in Figure 8.
As shown in Figure 8, adding under the action of a magnetic field, the oscillation frequency that is modified with magnetic material QCM (Quartz Crystal Microbalance) when not providing externally-applied magnetic field of mouse immunoglobulin (Ig) (mouse IgG) is 41Hz, and oscillation frequency rose to 82Hz after externally-applied magnetic field was provided, and add 1.25% glutaraldehyde (glutaraldehyde with mouse immunoglobulin (Ig) functional group effect again, GA) after, the oscillation frequency of QCM (Quartz Crystal Microbalance) increases to 103Hz, this represents modified magnetic material when catching and catch the desire analyte, really can allow the oscillation frequency of QCM (Quartz Crystal Microbalance) change, verify technical feasibility of the present invention.And the variation that the oscillation frequency of described QCM (Quartz Crystal Microbalance) takes place can be learnt the content of desiring analyte after being converted by signal processor.
The experiment of embodiment 3. magnetic material detection mechanism
In the present embodiment, the magnetic particle of further having inquired into micron-scale is used for feasibility of the present invention; And in present embodiment, also considered that protein possibility non-specific adsorption is in the problem on acoustics sensing component surface, therefore before carrying out detection, earlier with bovine serum albumin (bovine serum albumin, BSA) be coated in acoustics sensing component surface (working concentration: 10mg/ml), thus, the efficiency of isolation to other protein can reach 90%.
Present embodiment adopts magnetic high-molecular particulate (the about 2.8 μ m of mean grain size that buy from Dynal Biotech company; Working concentration: 67 μ g/ml), the cooperation fluid-pouring-in (use flow velocity: 50 μ l/ml) the QCM (Quartz Crystal Microbalance) system experimentizes.The magnetic field source of supply also adopts a High-Field value magnet (the about 100mT of magnetic field intensity that acoustics sensing component surf zone is exposed).Show by Fig. 9 result, adding under the action of a magnetic field, be modified with magnetic material (the modification condition: 80 μ g/ml Escherichia coli antibody+67 μ g/ml magnetic materials of Escherichia coli antibody (anti-E.coli), the influence that is produced when about 1.5 hours of stirring reaction), its frequency change that causes is than magnetic material has more about 320Hz.
The present embodiment utilization is modified with the magnetic material of mouse immune globulin antibody (anti-mouseIgG) (available from Dynal Biotech company; Working concentration: 67 μ g/ml), directly to mouse immunoglobulin (Ig) (working concentration: 180 μ g/ml) detect.Experimental provision is set with embodiment 3 same, and its acoustics sensing component surface is coated with bovine serum albumin.The magnetic material that will be modified with the mouse immune globulin antibody before the detection earlier mixes with the mouse immunoglobulin (Ig), and about 1.5 hours of stirring reaction injects runner with it more afterwards and detects.Figure 10 has shown the difference before and after the reaction, and the frequency variation of 70Hz is arranged altogether approximately.Embodiment has illustrated that this is installed on the feasibility on the immune detection thus.
Comprehensively above-mentioned, the analytical approach of use magnetic particle of the present invention and device, utilize modified magnetic material to catch the test substance of desiring in the analytic sample, and providing externally-applied magnetic field that magnetic material is drawn to the induction region of acoustics sensing component, the effect with the induction region surface is scaled the content of desiring test substance in the analytic sample then.Therefore, the separating substances technology that analytical equipment of the present invention and method have been assembled magnetic particle have simple to operate rapidly, to biological substance or desire analyte and disturb advantages such as little, cheap, the high sensitivity characteristic that cooperates biology sensor, becoming one can detect and the quantitative detection technology of micro substance in real time, can be widely used in giving birth to fields such as doctor, environment and food analysis, diagnose the microanalysis inductor of cover group and mitogenetic, environment and food as giving birth to the doctor.
Other embodiment
Disclose several embodiments of the present invention in this manual.According to the disclosed content of this instructions, any those skilled in the art that scholar all can not breaking away under the present invention's spirit and the purpose, do different changes and modification to the present invention based on characteristic of the present invention, therefore, other embodiment is also contained in the claim of the present invention.
Claims (26)
1. analytical approach of using magnetic material is characterized in that step comprises:
A) provide a magnetic material, described magnetic material surface has the identification molecule that can catch test substance in the sample;
B) described magnetic material is added in the sample, make the identification molecule on described magnetic material surface catch test substance in the sample;
C) utilize a magnetic field that described magnetic material is directed to the induction region surface of an acoustics sensing component, make described induction region surface produce acoustic effect; And
D) described acoustic effect is scaled the content of test substance in the sample.
2. the method for claim 1 is characterized in that, described this magnetic material comprises magnetic particle.
3. the method for claim 1 is characterized in that, described this magnetic material comprises magnetic nano-particle.
4. the method for claim 1 is characterized in that, described this magnetic material comprises super paramagnetic nano particle.
5. the method for claim 1 is characterized in that, described this identification molecule inclusion compound.
6. the method for claim 1 is characterized in that, described this identification molecule comprises antigen, antibody, acceptor, part, ferment, protein, victory peptide or nucleic acid.
7. the method for claim 1 is characterized in that, described this acoustics sensing component comprises QCM (Quartz Crystal Microbalance), surface acoustic wave component, dull and stereotyped ripple assembly or micro-cantilever.
8. the method for claim 1 is characterized in that, described this acoustics sensing component comprises bent flat Lamb wave assembly.
9. the method for claim 1 is characterized in that, described this acoustic effect comprises deformation quantity variation, resonant frequency shift quantitative changeization or acoustic signals to be changed.
10. the method for claim 1 is characterized in that, further utilizes an optics sensing component to detect described magnetic material.
11. method as claimed in claim 9 is characterized in that, described this magnetic material surface further has a signaling molecule, and this signaling molecule has the characteristic of light absorption, fluorescent radiation, phosphorescence radiation or cold light radiation.
12. the device of test substance is characterized in that in the analytic sample, comprises:
One reactive tank, this reactive tank have the usefulness of a space as the sample importing;
One magnetic material, this magnetic material surface have the identification molecule that can catch test substance in the described sample;
One magnetic field producer is in order to provide the magnetic material in the described reactive tank one externally-applied magnetic field; And
One acoustics sensing component, this acoustics sensing component has an induction region that is exposed in the described reactive tank;
Wherein said device is to utilize externally-applied magnetic field that magnetic field producer provides that the test substance of described magnetic material and seizure thereof is focused to the induction region surface of described acoustics sensing component, makes the induction region surface produce acoustic effect and obtains test substance content in the sample to convert.
13. device as claimed in claim 12 is characterized in that, described this acoustics sensing component comprises QCM (Quartz Crystal Microbalance), surface acoustic wave component, dull and stereotyped ripple assembly or micro-cantilever.
14. device as claimed in claim 12 is characterized in that, described this acoustics sensing component comprises bent flat Lamb wave assembly.
15. device as claimed in claim 12 is characterized in that, described this magnetic material comprises magnetic particle.
16. device as claimed in claim 12 is characterized in that, described this magnetic material comprises magnetic nano-particle.
17. device as claimed in claim 12 is characterized in that, described this magnetic material comprises super paramagnetic nano particle.
18. device as claimed in claim 12 is characterized in that, described this identification molecule inclusion compound.
19. device as claimed in claim 12 is characterized in that, described this identification molecule comprises antigen, antibody, acceptor, part, ferment, protein, victory peptide or nucleic acid.
20. device as claimed in claim 12 is characterized in that, described this magnetic field producer comprises inductive coil or magnet.
21. device as claimed in claim 12 is characterized in that, described this induction region acoustic effect comprises deformation quantity variation, resonant frequency shift quantitative changeization or acoustic signals to be changed.
22. device as claimed in claim 12 is characterized in that, described this reactive tank is a container, with so that sample static state be stored in this container.
23. device as claimed in claim 12 is characterized in that, described this reactive tank is a pipeline, with so that sample dynamically circulate in this pipeline.
24. device as claimed in claim 12 is characterized in that, described this reactive tank further comprises a magnetic material collecting region, and this magnetic material collecting region is as described induction region setting wherein.
25. device as claimed in claim 12 is characterized in that, further comprises an optics sensing component, in order to detect described magnetic material.
26. device as claimed in claim 25 is characterized in that, described this magnetic particle surface further has a signaling molecule, and this signaling molecule has the characteristic of light absorption, fluorescent radiation, phosphorescence radiation or cold light radiation.
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| AU2009320152B2 (en) * | 2008-10-27 | 2015-01-29 | Advantageous Systems, Llc | Liquid purification using magnetic nanoparticles |
| CN104165819B (en) * | 2013-08-29 | 2016-06-08 | 北京至感传感器技术研究院有限公司 | A kind of online magnetic particle monitoring systems in real time |
| CN107110784A (en) * | 2014-06-12 | 2017-08-29 | N·H·沃尔克 | Optical biosensor |
| RU2689582C2 (en) * | 2014-10-24 | 2019-05-28 | Лайф Текнолоджиз Корпорейшн | Liquid-liquid purification system of sample with acoustic deposition |
| CN106018028B (en) * | 2016-06-03 | 2018-09-14 | 段学欣 | A kind of method and device for realizing the control and detection of determinand aggregation in solution |
| CN106706898B (en) * | 2016-11-23 | 2018-07-20 | 深圳沃德生命科技有限公司 | A kind of biological detecting system and its detection method |
| CN112014277A (en) * | 2019-05-28 | 2020-12-01 | 北京致感致联科技有限公司 | Portable magnetic particle detection equipment |
| CN116500290B (en) * | 2023-06-21 | 2023-09-08 | 长沙海纳光电科技有限公司 | Supersaturated solid medicament adding device and method based on transmission method |
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| 主动式生物芯片技术. 张旭等.微电子学，第33卷第4期. 2003 |
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