CN100591366C - Hemostatic, bone impairment renovation material with bioactivity - Google Patents
Hemostatic, bone impairment renovation material with bioactivity Download PDFInfo
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- CN100591366C CN100591366C CN200610157658A CN200610157658A CN100591366C CN 100591366 C CN100591366 C CN 100591366C CN 200610157658 A CN200610157658 A CN 200610157658A CN 200610157658 A CN200610157658 A CN 200610157658A CN 100591366 C CN100591366 C CN 100591366C
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- bone
- lecithin
- chitosan
- hemostasis
- impairment renovation
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Abstract
The invention relates to a material used to stop blooding and repair the skeleton, with biological activity. The invention is characterized in that it comprises 45-60% biological active glass nanometer powder, 25-40% of collagen, 8-15% of chitose, and 8-15% of lecithin, to be mixed, crosslink, frozen and dried in vacuum. The invention has the functions as blooding stop and skeleton repair, withouttoxicity while it is degradable in sponge or powder shape. The invention has wide application.
Description
Technical field
The present invention relates to a kind of bioactive hemostasis and bone impairment renovation material, belong to the bio-medical material technical field.
Background technology
The local hemostasis material is widely used in surgical hemostasis, and the research of local hemostasis medicine and application in recent years had development rapidly.Yet damaged wound takes place in, osseous tissue dark for wound, and Kai Fa material was not good enough at aspect biological functions such as hemostasis, promotion healings in recent years, and did not possess the function of the damaged quick reparation of bone.Therefore exploitation uses functional hemostasis of new generation, bone renovating material to remain a worldwide difficult problem so far.
Bioactivity glass is that a class is widely used in the inorganic active material that bone is repaired.They have excellent biological compatibility, have no side effect, and because their chemical composition is similar to the natural skeleton of organism, therefore easily with the chemical bonding of the close and firm of skeleton formation on every side, or form new skeleton composition through biodegradation.Simultaneously, bioactivity glass also has Blood clotting, has been widely used as dental materials.
Collagen protein is the main organic principle of body bone tissue, has higher cellular affinity, can with platelet generation hemagglutinative function, be widely used aspect bone reparation and the hemostatic material.Therefore, with bioactivity glass and compound bone repairing performance and the anthemorrhagic performance that is expected to improve material monolithic of collagen.
For further improving biological activity by the composite that bioactivity glass, collagen protein constituted, strengthen its bone repairing effect, can consider to carry out compound with another kind of natural biological molecule such as lecithin.The lecithin materials source is abundant, and its contained phosphoprotein is the important composition composition of natural bone tissue and cell biological membrane structure, is the important regulator of osseous tissue generation biomineralization.
Chitosan is the derivant of chitin, and raw material sources are abundant, and are cheap, has hemostasis, antibacterial, antibiotic, biocompatibility, promotion wound healing and is easy to form the function of gel.The Blood clotting of chitosan does not rely on thrombin and platelet, thereby can be used for having patient's hemostasis of dysfunction of platelet.The sthptic sponge that present domestic existing chitosan is made is for clinical use.In the composite of forming by bioactivity glass, collagen protein and lecithin, add chitosan, can improve the hemostatic function of composite.
The advantage that the objective of the invention is to comprehensive organism activity glass, collagen protein, chitosan and lecithin, prepare a kind of novel, cheaply, the hemostasis of biologically active, bone renovating material, when possessing anastalsis, have the function of bone defect repair concurrently.
Up to the present, do not see have with bioactivity glass, collagen protein, chitosan and lecithin Application of composite domestic and international patent documentation report in blood coagulation material, treatment of wounds and bone wound defect repair.
Summary of the invention
The present invention can prepare has the active hemostasis of good biological, bone impairment renovation material.Purpose of the present invention realizes by following measure:
1. a bioactive hemostasis, bone impairment renovation material is characterized in that:
The chemical composition of material and quality percentage composition thereof are: bioactive glass nano powder 45～60%, collagen protein 25～40%, chitosan 8～15%, lecithin 8～15%.2. the preparation method of a bioactive hemostasis, bone impairment renovation material, concrete steps are as follows:
(1) is 45～60% bioactive glass nano powders, 25～40% collagen solution, 8～15% chitosan powder body, 8～15% lecithin mixing with mass percent, stirs.
(2) being buffer agent with ethylene sulfonic acid, is that cross-linking agent carries out crosslinking Treatment to mixed solution with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide of 1～3mg/ml and the N-hydroxy-succinamide of 0.2～1.0mg/ml, and the regulator solution pH value is 4～7.
(3) with mixed solution-20～40 ℃ freezing 15～25 hours down, and under this temperature vacuum drying.
(4) drying sample was washed 30 minutes repeatedly with deionized water, dry under vacuum state again.
Advantage of the present invention
(1) good biological activity and biocompatibility.Bio-vitric is highly active nano-powder, and collagen protein, chitosan and lecithin all are the natural biological molecular materials, so this composite is nontoxic, and degradable absorbs, and has good biological activity and biocompatibility.
(2) good bone repairing effect.Its composition of bioactivity glass is CaO-P
2O
5-SiO
2System, similar to the inorganic constituents of body bone tissue, have the characteristic of bone guided and promotion bone lengthening; Collagen protein is the main organic principle of body bone tissue, has the biological characteristics of induced tissue cell growth; Phosphoprotein in the lecithin is the important composition composition of natural bone tissue and cell biological membrane structure, is the important regulator of biomineralization, and is essential by cell differentiation, propagation and regeneration, can keep and promote the biological activity of multiple embrane-associated protein and receptor.Therefore, this composite has good synosteosis power and cellular affinity, can promote differentiation, propagation, adhesion, the maturation of osteoblast, chondroblast, generates osteoid and then mineralising, accelerates the healing of bone wound.
(3) good hemostatic function.Bioactivity glass, collagen protein and chitosan all have coagulation function, and the anastalsis of chitosan do not rely on thrombin and platelet, so this material can be issued to the hemostatic purpose in the situation of dysfunction of platelet.
(4) has anti-microbial property.Bioactivity glass and chitosan have the ability of control antibacterial in its surface transplantation growth.
(5) can be prepared into spongy as required or powdery, convenient clinical use.
The specific embodiment
Embodiment 1
Select for use bioactive glass nano powder, collagen solution, chitosan powder body and lecithin to prepare hemostasis, the bone impairment renovation material of biologically active.Its preparation process is as follows:
(1) bioactive glass nano powder, collagen solution, chitosan powder body and lecithin are mixed in proportion, stir.The quality percentage composition of each composition is respectively: bioactive glass nano powder 50%, collagen protein 30%, chitosan 10%, lecithin 10%.
(2) in the mixed solution of bioactive glass nano powder, collagen solution, chitosan powder body and lecithin, be buffer agent with ethylene sulfonic acid, with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide of 2mg/ml and the N-hydroxy-succinamide of 0.5mg/ml is that cross-linking agent carries out crosslinking Treatment to mixed solution, and the regulator solution pH value is 5.。
(3) mixed solution of above-mentioned crosslinked mistake is inserted-30 ℃ of freezing 15h of fridge.
(4) freezing sample is carried out vacuum drying treatment under-30 ℃.
(5) drying sample was washed 30 minutes repeatedly with deionized water, dry under vacuum state again, obtain spongy solid material.The aperture mainly changes between 100～300 μ m, and porosity is more than 80%.
(6) material is standby with gamma-rays sterilization back.
Embodiment 2
Select for use bioactive glass nano powder, collagen solution, chitosan powder body and lecithin to prepare hemostasis, the bone impairment renovation material of biologically active.Its preparation process is as follows:
(1) bioactive glass nano powder, collagen solution, chitosan powder body and lecithin are mixed in proportion, stir.The quality percentage composition of each composition is respectively: bioactive glass nano powder 55%, collagen protein 25%, chitosan 12%, lecithin 8%.
(2) in the mixed solution of bioactive glass nano powder, collagen solution, chitosan powder body and lecithin, be buffer agent with ethylene sulfonic acid, with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide of 1.5mg/ml and the N-hydroxy-succinamide of 0.4mg/ml is that cross-linking agent carries out crosslinking Treatment to mixed solution, and the regulator solution pH value is 5.5.
(3) mixed solution of above-mentioned crosslinked mistake is inserted-25 ℃ of freezing 20h of fridge.
(4) freezing sample is carried out vacuum drying treatment under-25 ℃.
(5) drying sample was washed 30 minutes repeatedly with deionized water, dry under vacuum state again, obtain bulk material.
(6) the bulk material pulverizing is obtained the powder body material that particle diameter changes at 1 μ m～20 mu m ranges.
(7) material is standby with gamma-rays sterilization back.
Claims (3)
1. a bioactive hemostasis, bone impairment renovation material, it is characterized in that: the chemical composition of material and quality percentage composition thereof are: bioactive glass nano powder 50%, collagen protein 30%, chitosan 10%, lecithin 10%.
2. hemostasis according to claim 1, bone impairment renovation material is characterized in that its pattern is spongy or powdery.
3. hemostasis according to claim 1, bone impairment renovation material, its preparation method is specific as follows:
(1) bioactive glass nano powder, collagen solution, chitosan powder body and lecithin are mixed in proportion, stir;
(2) be buffer agent with ethylene sulfonic acid (MES), regulating pH value is 5～7, is that cross-linking agent carries out crosslinking Treatment to mixed solution with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) of 1～3mg/ml and the N-hydroxy-succinamide (NHS) of 0.2～1mg/ml; (3) with mixed solution-20～-40 ℃ freezing 15～25 hours down, and under this temperature vacuum drying; (4) drying sample was washed 30 minutes repeatedly with deionized water, dry under vacuum state again, make spongiform material; (5) spongy drying sample is pulverized, made dusty material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610157658A CN100591366C (en) | 2006-12-15 | 2006-12-15 | Hemostatic, bone impairment renovation material with bioactivity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610157658A CN100591366C (en) | 2006-12-15 | 2006-12-15 | Hemostatic, bone impairment renovation material with bioactivity |
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| Publication Number | Publication Date |
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| CN101011598A CN101011598A (en) | 2007-08-08 |
| CN100591366C true CN100591366C (en) | 2010-02-24 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991875B (en) * | 2010-10-29 | 2014-01-22 | 上海昊海生物科技股份有限公司 | Mesoporous bioactive glass and chitosan composite porous hemostatic material and preparation method thereof |
| CN102580148A (en) * | 2012-03-12 | 2012-07-18 | 华南理工大学 | Preparation method of composite stent for repairing bone defect |
| US9775853B2 (en) | 2013-03-15 | 2017-10-03 | Biomet Manufacturing, Llc. | Hemostatic compositions and methods |
| CN107715168A (en) * | 2017-10-18 | 2018-02-23 | 张家港蓝智生物科技有限公司 | A kind of functions of stanching and promoting healing uterine neck wound repair anhydrous gel of the microballoon containing bio-vitric |
| CN108245711A (en) * | 2018-02-26 | 2018-07-06 | 王由 | A kind of collagen compound 45S5 bioactivity glass and its preparation method and application |
| CN108273127A (en) * | 2018-04-23 | 2018-07-13 | 周荣 | A kind of preparation method of enzymolysis type bio-vitric dental pulp repair materials |
| CN112237550A (en) * | 2020-11-24 | 2021-01-19 | 武汉幸福益生再生医学科技有限公司 | Dental filling material of bioactive glass material and filling method thereof |
| CN114246984B (en) * | 2021-11-19 | 2022-10-14 | 浙江瑞谷生物科技有限公司 | Bone repair material with rhBMP-2 carried by gelatin sponge and preparation method thereof |
| CN114588313B (en) * | 2022-04-13 | 2023-03-24 | 北京纳通医疗科技控股有限公司 | Novel collagen and bioactive ceramic composite bone grafting material and preparation method thereof |
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