CN101120664B - Method and apparatus for inducing artificial hibernation of marine animal - Google Patents
Method and apparatus for inducing artificial hibernation of marine animal Download PDFInfo
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- CN101120664B CN101120664B CN2007100958650A CN200710095865A CN101120664B CN 101120664 B CN101120664 B CN 101120664B CN 2007100958650 A CN2007100958650 A CN 2007100958650A CN 200710095865 A CN200710095865 A CN 200710095865A CN 101120664 B CN101120664 B CN 101120664B
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A method and an apparatus for inducing artificial hibernation of marine animal, wherein the temperature of sea water with marine animals is gradually lowered in stepwise process, while the sea water temperature is kept as is for a predetermined period of time at each step of lowering temperature, whereby the predetermined period of time maintained for each step of the lowered temperature is gradually increased to a point where changes of oxygen consumption by the marine animals greatly decreased or the changes of oxygen consumption is reached almost zero, and starting from this point, the time of constantly maintaining the sea water temperature at each step is gradually reduced to thereby ensuring a long survival rate (viability) of marine animals.
Description
Technical field
The present invention relates to halobiontic artificial hibernation abductive approach and device thereof, its characteristics are, the ocean temperature that reduces marine organisms stage by stage and survived, and the temperature maintenance time of each temperature-fall period progressively prolonged.
Background technology
Rapid development along with the cultural technique eighties in last century, since the beginning of the nineties, the domestic senior breed sashimi (raw fish) of Korea S produces and constantly increases (fish-farming in shallow marine water fish in 1997 total growth is 39121 tons), and along with the increase of national income, live fish consumption also significantly increases.
But at present Korea S is domestic when transportation comprises the marine organisms of live fish, relies on low-density transportation technologies such as live fish special-purpose vehicle (account for full weight amount 15～20%) to transport.Like this, the haulage time that traffic jam causes during because of long-distance transport prolongs, and cause being difficult to keep the freshness of fish, and its cost of transportation is too high, and fish is fresh inadequately.
At present, marine organisms transportation resources such as live fish are divided into substantially, (1) anesthesia conveying method, (2) electric shock shock conveying method, (3) bosh conveying method, (4) artificial hibernation conveying method etc.For example, Korean granted patent (the patent No.: 10-0232408) disclose a kind of high density live fish transportation resources of using refrigerated sea water, it is characterized by, reduce ocean temperature during transport of living fish, by in fish well, putting into seawater, ice cube, salt, during transportation with ocean temperature than low 5～15 ℃ of general transport temperature.Korean granted patent (10-0531728) relates to the ice temperature seawater cooling device that inkfish transportation and storage alive is used, and its ice temperature seawater cooling device can be under the low temperature of ice temperature state, and inkfish takes care of and transports to living.Korean granted patent (grant number: 10-0046109) relate to live fish transportation store method and device,, be provided with a plurality of holes of accommodating one or more live fish, and keep low temperature, thereby suppress the activity of fish in order not make the damage of live fish body surface.
But, above-mentioned existing method all is that the temperature that reduces live fish carrier is basic ideas, and the bosh conveying method of this use live fish carrier has bosh expense height, can't adapt to the special vehicle of difference, needs of each fingerling low temperature physiological property, transport for a long time and cause shortcomings such as halobiontic survival rate is low.In addition, above-mentioned anesthesia conveying method might cause people to the misgivings on the edible safety, and electric shock shock conveying method is because of existing operation easier big, causes degradation problem under the qualities of the death by suffocation of fish and fish, so be difficult to apply.
Summary of the invention
The present invention is intended to address the above problem, its purpose is, include halobiontic ocean temperature by reducing stage by stage, at each temperature-fall period, ocean temperature is kept special time, and the thermostatic hold-time of above-mentioned each temperature-fall period, till the variation that progressively increases to the oxygen demand that marine organisms consume obviously reduces or does not almost change, then with this as the starting point, gradually reduce the thermostatic hold-time in above-mentioned each temperature-fall period, thereby keep halobiontic high-servival rate for a long time.
That is, the objective of the invention is to, induce marine organisms to enter hibernation artificially, and the above-mentioned marine organisms that enter hibernation are put into packing case with anhydrous state (Waterless condition), survive for a long time to keep marine organisms.The invention provides a kind of method, be different from existing live fish transportation resources, specifically be that all biologies in the water are object, fully understanding on the biological interior basis in biorhythm, induce anhydrous state artificially through multistage variations in temperature, halobiontic inherent biorhythm is stopped for a long time, return to the brand new technical of original biorhythm again.
In order to achieve the above object, halobiontic artificial hibernation abductive approach of the present invention is characterized in that, comprising: place marine organisms in the seawater and keep the step of special time; The interval reduces above-mentioned ocean temperature by stages according to the rules, and progressively increases the step of the thermostatic hold-time of above-mentioned each temperature-fall period at above-mentioned each temperature-fall period; And the ocean temperature during with amount of oxygen zero deflection that above-mentioned marine organisms were consumed is starting point, gradually reduces the step of the thermostatic hold-time of above-mentioned each temperature-fall period.
In order to achieve the above object, another form of implementation of the present invention, promptly halobiontic artificial hibernation apparatus for deivation is characterized in that, comprising: the artificial hibernation case fills and comprises halobiontic seawater; Heat exchanger or cooler, the temperature of the seawater that will discharge from above-mentioned artificial hibernation case is back to seawater in the artificial hibernation case according to after reducing stage by stage between given zone again; Measure the temperature sensor of the ocean temperature in the above-mentioned artificial hibernation case, and the lambda sensor that is used for measuring the residual amount of oxygen of above-mentioned seawater; Computer, control above-mentioned heat exchanger or cooler, according to water temperature information from the said temperature sensor, above-mentioned ocean temperature through cooling is stage by stage kept special time, progressively increase the thermostatic hold-time of above-mentioned each temperature-fall period, and according to from the residual amount information of oxygen in the seawater of above-mentioned lambda sensor, when deviation (variation) did not appear in the amount of oxygen that consumes from above-mentioned marine organisms, the ocean temperature that gradually reduces above-mentioned each temperature-fall period was held time.
According to the present invention, include halobiontic ocean temperature by the stage reduction, every temperature-fall period, ocean temperature is kept special time, will be at the thermostatic hold-time of above-mentioned each temperature-fall period, till the variation that progressively increases to halobiontic oxygen demand significantly reduces or does not almost change, then with this as the starting point, gradually reduce the thermostatic hold-time in above-mentioned each temperature-fall period, thereby can keep halobiontic high-servival rate for a long time.
Description of drawings
Fig. 1 is according to the ocean temperature that reduces stage by stage, progressively to increase the first embodiment schematic diagram of temperature maintenance time among the present invention;
Fig. 2 is according to the ocean temperature that reduces stage by stage, progressively to increase the second embodiment schematic diagram of temperature maintenance time among the present invention;
Fig. 3 is among the present invention, can measure the structural representation of the Self-breathing determinator of halobiontic oxygen demand;
Fig. 4, Fig. 5 are respectively, halobiontic breathing state of the breed of measuring among the present invention and the circadian rhythm curve synoptic diagram that draws in view of the above;
Fig. 6, Fig. 7 are respectively, wild halobiontic breathing state of measuring among the present invention and the morning and evening tides rhythm and pace of moving things curve synoptic diagram that draws in view of the above;
Fig. 8 is, among the present invention, the variation that is used for determining the marine organisms oxygen demand obviously reduce or oxygen demand almost the ocean temperature during no change, along with ocean temperature changes and the marine organisms oxygen demand curve synoptic diagram that changes;
Fig. 9 is among the present invention, progressively to increase the curve synoptic diagram of temperature maintenance time along with reducing ocean temperature stage by stage;
Figure 10, Figure 11 are respectively, the end view and the vertical view of the artificial hibernation device among another embodiment of the present invention.
Embodiment
Below, with reference to accompanying drawing desirable embodiment of the present invention is elaborated.
The present invention is used for marine organisms are carried out artificial hibernation, it is characterized in that reducing stage by stage comprising halobiontic ocean temperature, and the ocean temperature that progressively prolongs in each temperature-fall period is held time.
For this reason, the present invention puts into marine organisms seawater and keeps special time earlier.That is, prepare to comprise halobiontic seawater.Here, except seawater, also can use fresh water or other water.For convenience of explanation, below be that example describes, but the present invention can use fresh water and other any type of water except seawater with the seawater.
Secondly, the present invention interval according to the rules reduces above-mentioned ocean temperature stage by stage, progressively increases the thermostatic hold-time in above-mentioned each temperature-fall period temperature.That is, reduce the ocean temperature of above-mentioned preparation stage by stage, and every temperature-fall period, keep this phase temperature of special time, and the thermostatic hold-time of each temperature-fall period is progressively prolonged.
Existing marine organisms transportation resources, major part all are the methods that live fish is maintained low-temperature condition or cools off with ice cube.And in the present invention, induce halobiontic artificial hibernation by reducing water temperature every specific interval.For example, shown in Figure 1, the ocean temperature of above-mentioned preparation is 13 ℃ and has kept on this temperature 10 minutes, reduce water temperature stage by stage according to 1 ℃ of interval then, temperature at above-mentioned each temperature-fall period, promptly in 12 ℃, 11 ℃, 10 ℃, 9 ℃, will include halobiontic ocean temperature progressively extend to 20 minutes respectively, 30 minutes, 40 minutes and 50 minutes.
Confirmed across specified time interval by the present invention, reduced the seawater water temperature stage by stage, the ocean temperature that progressively increases each temperature-fall period is simultaneously held time, and can effectively induce marine organisms to enter artificial hibernation.If once cool to temperature requiredly, marine organisms might be dead because of low temperature shock.The present invention regulates water temperature and time stage by stage, so that marine organisms adapt to low temperature if having time.
In the present invention, as shown in Figure 1, above-mentioned water temperature is reduced stage by stage according to 1 ℃ of temperature range.Also can be 2 ℃, 3 ℃ or abovely lower the temperature, progressively to be increased in the thermostatic hold-time of each temperature range then as a temperature range.Specifically be, be 13 ℃ and in this temperature, kept 10 minutes, on 11 ℃, kept 20 minutes so, kept 30 minutes on 9 ℃ as the seawater water temperature.In addition, as mentioned above, will include halobiontic ocean temperature and reduce by stages, and progressively prolong water temperature retention time, along with the time changes ocean temperature and changes at each temperature-fall period.Also can be as described in Figure 2,, between two ocean temperatures that adjoin each other overlapping part to be arranged in the part-time interval.
As mentioned above, after the ocean temperature that reduces ocean temperature stage by stage and prolong each temperature-fall period is held time, with halobiontic oxygen demand change obviously reduce or almost the ocean temperature during no change be starting point, the ocean temperature that begins to gradually reduce in above-mentioned each temperature-fall period is held time.Specifically be, according to halobiontic breathing state or inherent biorhythm, grasp after marine organisms are subjected to ocean temperature stress be maximum the time, be set in the holding time of above-mentioned ocean temperature interval to the longest, stress (Stress) with what reduce farthest that marine organisms are subjected to.
Marine organisms in the seawater weaken gradually along with water temperature reduction breathing and are irregular, and under specified temp, halobiontic oxygen demand significantly reduces or almost do not change.This shows that halobiontic breathing biorhythm marked change has taken place or stops substantially, and in this temperature range, what marine organisms were suffered stress be maximum.Therefore, the present invention is benchmark with the specified temp, holding time in the ocean temperature when halobiontic oxygen demand variation is obviously reduced or almost do not change set the most longly, the constant temperature that reduces stage by stage in the subsequent stage is held time then, like this, reduce as far as possible marine organisms suffered stress situation under, induce halobiontic artificial hibernation.
Above-mentioned when being benchmark with the specified temp, halobiontic oxygen demand changes obviously minimizing or does not almost have variation to be meant, in each ocean temperature that reduces stage by stage, the variation of marine organisms institute oxygen consumed tolerance, promptly the maximum of oxygen demand and the deviation between the minimum of a value (amplitude) are than obviously minimizing or its deviation were small in the past.Halobiontic oxygen demand amplitude measured in specified temp is little, shows that halobiontic respiratory activity reduces in response to swashing.And measured halobiontic oxygen demand amplitude is big in specified temp, shows that then halobiontic respiratory activity is active, and these can change and confirm by measuring the amount of oxygen that is contained in the seawater.
As mentioned above, place in the seawater of particular water temperature in the incipient stage and to have the marine organisms of the 1st oxygen demand and to keep special time, then above-mentioned ocean temperature interval is according to the rules reduced stage by stage, and in above-mentioned each temperature-fall period, keep this temperature-fall period ocean temperature of special time, and progressively increase the thermostatic hold-time of each temperature-fall period.And another embodiment of the present invention is, in above-mentioned each temperature-fall period temperature, when above-mentioned halobiontic oxygen demand be above-mentioned the 1st oxygen demand 1/1.5th to 1/4th the time, perhaps be in 6.0ml O when the deviation of above-mentioned halobiontic oxygen demand
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1Ocean temperature in the time of in the scope is a starting point, reduces the thermostatic hold-time in above-mentioned each temperature-fall period gradually.
Among the present invention, in order to grasp the halobiontic oxygen demand deviation under the specified temp, be necessary in implementation process of the present invention or before, measure halobiontic breathing state, so the present invention also can comprise the process that these halobiontic breathing states are measured.For example,, know above-mentioned halobiontic oxygen demand, in the present invention, also can use the Self-breathing determinator that to measure the marine organisms breathing state by measuring to including the amount of oxygen that dissolves in the halobiontic seawater.
In order to grasp halobiontic breathing state, can select to use the Self-breathing determinator (Korean Patent Publication No.: 10-2003-0075931) of also having authorized by inventor's application.As shown in Figure 3, this device comprises: the closed major circulatory system that makes water circulation; Be communicated in above-mentioned major circulatory system, be used to accommodate the biological bin of tested biology; Be connected in parallel on above-mentioned major circulatory system, the auxiliary circulation system of the supplementing water that contains saturated dissolved oxygen is provided to above-mentioned major circulatory system; Measure the dissolved oxygen in the above-mentioned major circulatory system, and connect the s operation control portion of above-mentioned auxiliary circulation system and major circulatory system, this device can compensate dissolved oxygen concentration and atmospheric pressure automatically, thereby can measure biological breathing state for a long time.By to above-mentioned Self-breathing determinator of having authorized, the method for measuring halobiontic breathing state is elaborated.In addition, use other method well known in the art or device to measure halobiontic breathing state, also should belong within the scope of the invention.
Among the present invention, can grasp halobiontic inherent biorhythm by the halobiontic breathing state result of said method mensuration.Above-mentioned halobiontic biorhythm is preferably under unglazed, as to have certain moisture and the salinity experiment condition and obtains.In possessing the experiment container of above-mentioned experiment condition, the dissolved oxygen variable condition that the continuous automatic measurement marine organisms are consumed is grasped the mean value of dissolved oxygen variable quantity in the certain hour, thereby is grasped above-mentioned halobiontic inherent biorhythm.At this moment, the variation of the atmospheric pressure that can have a significant impact oxygen in water tolerance tool as required, water temperature, salt concentration compensates automatically.For example, from above-mentioned halobiontic breathing state measurement result, it still is the morning and evening tides rhythm and pace of moving things that above-mentioned as can be known halobiontic metabolic activity has circadian rhythm.Specifically be that Fig. 4 is the respiratory physiology activity curve figure of the flatfish that the flatfish METHOD FOR CONTINUOUS DETERMINATION of the fish farm being cultured by the Self-breathing determinator drew in six days.Use rhythm and pace of moving things time series analysis program, the above-mentioned halobiontic rhythm and pace of moving things is analyzed according to the data of above-mentioned METHOD FOR CONTINUOUS DETERMINATION.Like this, can draw as shown in Figure 5 the circadian result that tends to.Fig. 6 is for being object with the wild flatfish that catches, the measurement result of METHOD FOR CONTINUOUS DETERMINATION respiratory physiology activity; Fig. 7 for wild flatfish is analyzed by the rhythm analysis program, show that marine organisms have the morning and evening tides rhythm and pace of moving things under the wild state.When measuring marine organisms inherence biorhythm, can utilize specific computer system or program, also can use other method well known in the art to measure by halobiontic breathing state result.
The invention is characterized in, according to halobiontic breathing state or inherent biorhythm, grasp marine organisms be subjected to stress the degree maximum ocean temperature, and hold time the marine organisms in this water temperature zone and to set the most longly.It still is the morning and evening tides rhythm and pace of moving things that these features have circadian rhythm to those, or has circadian rhythm simultaneously and morning and evening tides rhythm and pace of moving things marine organisms are all applicable.
Fig. 8 is, is used among the present invention determining that halobiontic oxygen demand changes obviously reduces or the schematic diagram of the ocean temperature during no change almost, shows that having the halobiontic average oxygen demand that 24 hours circadian flatfish changes along with ocean temperature changes.
As shown in the figure, halobiontic average oxygen demand progressively reduces and reduces along with ocean temperature, in fact, halobiontic oxygen demand deviation at a certain temperature, promptly, during as benchmark, the maximum of halobiontic oxygen demand and the difference of minimum of a value also reduce along with the reduction of ocean temperature with a certain temperature.This be because, along with the reduction of water temperature, halobiontic activity begins to reduce, the amount of oxygen that marine organisms consumed also and then reduces or consumes oxygen hardly.
Halobiontic oxygen demand reduces or does not almost have an oxygen demand, mean that variation has taken place halobiontic inherent biorhythm, and as shown in Figure 8, ocean temperature when this variation taken place is, when the about 13 ℃ seawater of serviceability temperature, near the respiratory physiology metabolism of flatfish begin to weaken from normal condition 10 ℃ (A Fig. 8); Along with water temperature reduces, breathe (B among Fig. 8) near 6 ℃ of biorhythm generation significant change; Inherent breathe 4 ℃ that biorhythm stops near (C among Fig. 8), even and reduce water temperature again, near the metabolism also do not change 2 ℃ (D among Fig. 8).
Therefore, in the another embodiment of the present invention, when reducing ocean temperature stage by stage, with 14 ℃ to 12 ℃ as the 1st temperature, with 7 ℃ to 5 ℃ as the 2nd temperature, and with 5 ℃ to 3 ℃ as the 3rd temperature.Be the 1st temperature with 13 ℃ preferably, 6 ℃ is the 2nd temperature, and 4 ℃ is the 3rd temperature.Can pass through above-mentioned the 1st temperature, the 2nd temperature and the 3rd temperature successively during enforcement, reduce ocean temperature, also can be directly to reduce to the 3rd temperature from above-mentioned the 1st temperature.
Promptly, one embodiment of the present invention's halobiontic artificial hibernation abductive approach, it is characterized by, the marine organisms that at first will have the 1st oxygen demand are placed in the seawater that water temperature is the 1st temperature and kept for the 1st time period, reduce temperature then gradually, in the 3rd temperature, above-mentioned ocean temperature was kept for the 3rd time period.Here, above-mentioned the 3rd temperature is, in above-mentioned halobiontic oxygen demand reduces to 1/3rd to 1/4th scopes of above-mentioned the 1st oxygen demand, and the temperature when promptly being in the 3rd oxygen demand; And above-mentioned the 3rd time period is, the time in 10 times to the 100 times scopes of above-mentioned the 1st time period length.
Table 1 and shown in Figure 1 in the embodiment of the invention, will have 12.2ml O as described later
2Kg
-1WW h
-1The marine organisms of the 1st oxygen demand be placed in 13 ℃ of seawater and kept 10 minutes, then progressively reduce above-mentioned ocean temperature, reach 0.1ml O in halobiontic oxygen demand deviation
2Kg
-1WW h
-1, and inherent biorhythm is in 4 ℃ of halted state, keeps 260 minutes.That is, be equivalent to keep 10 times of the 3rd time periods that are equivalent to above-mentioned the 1st time period in 1/3rd to 1/4th the 3rd temperature of above-mentioned the 1st oxygen demand to 100 times of time spans in its halobiontic oxygen demand.Above-mentioned the 3rd temperature is that halobiontic oxygen demand does not almost change, suffered stress maximum zone, because of in this zone maintenance maximum duration, thus can significantly reduce to marine organisms bring stress.
As mentioned above, can comprise the steps: in water temperature to be to place in the seawater of the 1st temperature to have the marine organisms of the 1st oxygen demand and kept for the 1st time period in one embodiment of the invention; Reduce above-mentioned ocean temperature gradually, in the 2nd temperature, above-mentioned ocean temperature was kept for the 2nd time period (above-mentioned the 2nd temperature is 1/1.5th to 1/2.5th scopes that above-mentioned halobiontic oxygen demand is in above-mentioned the 1st oxygen demand, temperature when promptly being in the 2nd oxygen demand, and above-mentioned the 2nd time period be 10 times to 25 times the time span of above-mentioned the 1st time period); Reduce above-mentioned seawater water temperature gradually, in the 3rd temperature, above-mentioned water temperature was kept for the 3rd time period (above-mentioned the 3rd temperature is 1/3rd to 1/4th scopes that above-mentioned halobiontic oxygen demand is in above-mentioned the 1st oxygen demand, temperature when promptly being in the 3rd oxygen demand, and above-mentioned the 3rd time period be 26 times to 100 times the time span of above-mentioned the 1st time period).
Please refer to table 1 and Fig. 1 in the specification, will have 12.2ml O
2Kg
-1WW h
-1The marine organisms of the 1st oxygen demand be placed in 13 ℃ of seawater and kept 10 minutes, reach 9.8ml O in halobiontic average oxygen demand
2Kg
-1WW h
-1, and in obvious 6 ℃ of reducing of its inherent biorhythm, keep 120 minutes, and reach 6.2ml O in halobiontic average oxygen demand
2Kg
-1WW h
-1, and its inherent biorhythm is in halted state substantially, and (amplitude is 0.1ml O
2Kg
-1WW h
-1) 4 ℃ in, kept 260 minutes.That is, during above-mentioned the 2nd temperature, halobiontic oxygen demand changes significantly and reduces, and by kept the 2nd temperature of certain hour before the 3rd temperature, can make the marine organisms existence longer time.
In addition, another embodiment of the present invention comprises the steps: in water temperature to be to place in the seawater of the 1st temperature to have the marine organisms of the 1st oxygen demand and kept for the 1st time period; Progressively reduce ocean temperature poor to above-mentioned halobiontic oxygen demand maximum and minimum of a value then, promptly the biorhythm amplitude of oxygen consumption is at 1.1ml O
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1The 3rd temperature in the time of in the scope in above-mentioned the 3rd temperature, is kept above-mentioned seawater the time in 10 times to the 100 times scopes of above-mentioned the 1st time period.With reference to table 1 and Fig. 1, when the maximum of above-mentioned halobiontic oxygen demand and the difference of minimum of a value are in 1.1ml O
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1The 3rd temperature during scope is about 4.5 ℃ to 1 ℃.At this moment, above-mentioned the 3rd temperature is that the marine organisms oxygen demand does not almost have the zone that changes, suffered stress be also maximum, but because of keeping maximum duration in this zone, thus can significantly reduce bring halobiontic stress.
As mentioned above, another embodiment of the present invention is, places to have the marine organisms of the 1st oxygen demand and kept for the 1st time period in water temperature is the seawater of the 1st temperature; Progressively reduce ocean temperature extremely then, poor (the biorhythm amplitude of oxygen consumption) of the maximum of above-mentioned halobiontic oxygen demand and minimum of a value is in 6.0ml O
2Kg
-1WW h
-1To 1.0ml O
2Kg
-1WW h
-1The 2nd temperature in the time of in the scope, and in above-mentioned the 2nd temperature, above-mentioned seawater is kept 10 times of the 2nd time periods to 25 times of scope time spans that are equivalent to above-mentioned the 1st time period; Progressively above-mentioned ocean temperature is reduced to the maximum of above-mentioned marine organisms oxygen demand and the difference of minimum of a value and is in 1.1ml O
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1The 3rd temperature in the time of in the scope, and in above-mentioned the 3rd temperature, above-mentioned seawater is kept 26 times of the 3rd time periods to 100 times of scope time spans that are equivalent to above-mentioned the 1st time period.According to the table 1 in the embodiment of the invention and Fig. 1, the maximum of above-mentioned halobiontic oxygen demand and the difference of minimum of a value are in 6.0ml O
2Kg
-1WW h
-1To 1.0ml O
2Kg
-1WW h
-1The 2nd temperature in the time of in the scope is about 7.0 ℃ to 4.5 ℃ scopes, and above-mentioned the 2nd temperature is halobiontic oxygen demand and changes the zone of obviously reducing, and by kept the 2nd temperature of special time before the 3rd temperature, can make the marine organisms existence longer time.
That is, in the various embodiments described above of the present invention, when progressively reducing above-mentioned ocean temperature,, and when reaching above-mentioned each temperature-fall period temperature, progressively increase thermostatic hold-time preferably according to reducing above-mentioned ocean temperature between given zone stage by stage.
In addition, among each embodiment of the invention described above, above-mentioned marine organisms are preferably live fish or flatfish.
Another embodiment of the present invention is, is used in the artificial hibernation device of halobiontic artificial hibernation, and Figure 10 and Figure 11 are respectively the end view and the vertical view of the artificial hibernation device among another embodiment of the present invention.
As shown in the figure, another embodiment of the present invention is halobiontic artificial hibernation apparatus for deivation, it is characterized in that, comprising: artificial hibernation case 15, be equipped with and comprise halobiontic seawater; Heat exchanger 2 or cooler 5, the temperature of the seawater that will discharge from above-mentioned artificial hibernation case is back to seawater in the artificial hibernation case according to after reducing stage by stage between given zone again; Measure the temperature sensor 11,12 of the ocean temperature in the above-mentioned artificial hibernation case, and the lambda sensor 10 that is used for measuring the residual amount of oxygen of above-mentioned seawater; Computer 9, control above-mentioned heat exchanger or cooler, make it according to water temperature information from the said temperature sensor, keep the ocean temperature special time at each temperature-fall period, and progressively increase the thermostatic hold-time of above-mentioned each temperature-fall period, and according to from dissolved oxygen tolerance information in the seawater of above-mentioned lambda sensor, when deviation (variation) does not appear in above-mentioned marine organisms oxygen demand, according to above-mentioned each temperature-fall period, the ocean temperature that gradually reduces above-mentioned temperature-fall period is held time.
Above-mentioned artificial hibernation apparatus for deivation is controlled the operation of heat exchanger 2 or cooler 5 by above-mentioned computer 9, thereby the marine organisms in the artificial hibernation case 15 are carried out artificial hibernation.For this reason, the present invention's artificial hibernation apparatus for deivation also can comprise: the compressor 3 and the pressure control part 4 that are used for heat exchanger 2 or cooler 5 operations, also comprise the temperature control part 7 that to control the said temperature sensor, above-mentioned computer 9 can be controlled above-mentioned heat exchanger 2 or cooler 5 by above-mentioned pressure control part 4 and temperature control part 7 are controlled.
Below, above-mentioned artificial hibernation apparatus for deivation is described.
The present invention's artificial hibernation apparatus for deivation can use seawater and fresh water, so the pipeline that contacts with water and each member have used the titanium metal material of PVC material or seawater corrosion resistance.This artificial hibernation apparatus for deivation is substantially by artificial hibernation case 15 and cooling device and water temperature regulation device (computer) formation.
The inside and outside wall of above-mentioned artificial hibernation case 15 is made by plastics or titanium, is not subjected to seawater corrosion with the protection casing.For with external insulation, the mid portion between the inside and outside wall (thickness 5cm) is inserted heat-barrier material-epoxy resin, other is provided with lid.The volume of artificial hibernation case 15 is 500 liters, can adorn 400 liters water.The small water temperature of artificial hibernation case 15 inside changes can pass through Pt100 temperature sensor 11,12, and the value of computer 9 and 7 liang of places mensuration of temperature control part is compensated mutually.With lambda sensor (Multiline P4, WTW, Germany) 10 be connected on the computer 9, and regulate, remain on 100% to guarantee seawater dissolved oxygen (DO) degree of saturation by water inlet 14 and 13 pairs of seawater velocities of discharge outlet (45 liters/minute) of being located at artificial hibernation case 15 inside.
The present invention's artificial hibernation case 15 also can comprise fishing net 16, so that from seawater marine organisms are salvaged.For this reason, be preferably in the artificial hibernation case 15 and put into before the marine organisms, put into fishing net 16 earlier.Be attached to case when bottom as flat fishes such as flatfish, be difficult to catch, but when putting into flatfish in the fishing net 16, only need mention fishing net can catch.
Secondly, the cooling device in the present invention's the artificial hibernation apparatus for deivation comprises: per minute can be handled the pump 1 of 45 premium on currency; Capacity is the heat exchanger 2 of 12000Kcal/h; The compressor 3 of 2 horsepowers; Pressure control part 4; Cooler 5.In Figure 10 and Figure 11, represented to flow to through the seawater of this cooling device with solid line.When using above-mentioned cooling device to reduce the ocean temperature that flows in the above-mentioned artificial hibernation case 15, the cold-producing medium of circulation need keep high pressure and low temperature (25 ℃) between above-mentioned heat exchanger 2 and compressor 3 and pressure control part 4.In Figure 10, Figure 11, dotted the cyclic process of cold-producing medium.
At this moment, if low excessively through the ocean temperature of over-heat- exchanger 2,2 of heat exchangers might break down.Specifically be, the pipe diameter that seawater passes through in the heat exchanger (12000Kcal/h) is less, might cause to freeze in the pipe of heat exchanger 2 and cause heat exchanger 2 to break.Therefore, preferably, when the ocean temperature through the internal pipeline of over-heat-exchanger 2 was lower than specified temp, the pressure of regulating compressor 3 automatically to be reducing the temperature of cold-producing medium, thereby prevented that heat exchanger 2 from freezing.For example, when the water temperature of artificial hibernation case 15 inside is higher (as 13 ℃ to 3 ℃), the pressure that improves compressor 3 makes refrigerant temperature reach-25 ℃, and when the water temperature in the artificial hibernation case 15 is reduced to 1 ℃ of left and right sides, the pressure that then reduces compressor 3 makes refrigerant temperature reach-1 ℃, thereby prevents heat exchanger 2 freeze (freezing temperature when salinity 24.7 ‰ is-1.93 ℃).
In addition, the top of the present invention's artificial hibernation apparatus for deivation is provided with the light emitting diode of informing that cooling system is unusual, so that take measures rapidly when breaking down.The water temperature of hibernation induction room is automatically adjusted by the program in the predefined computer 9 by computer 9 and temperature control part 7.And show that by computer 9 artificial hibernation induces the water temperature in the process to regulate whether normally operation.Artificial hibernation induces program according to initial stage water temperature and fingerling, fish size, can change in the scope at 14～20 hours.
Above-mentioned artificial hibernation apparatus for deivation by the operation of above-mentioned computer 9 control heat exchangers 2 or cooler 5, thereby is implemented artificial hibernation to the marine organisms in the artificial hibernation case 15 automatically.For this reason, as mentioned above, above-mentioned computer 9 is according to the water temperature information from said temperature sensor 11,12, keep the ocean temperature special time at every temperature-fall period, and progressively increase the thermostatic hold-time of above-mentioned each temperature-fall period, and according to from dissolved oxygen tolerance information in the seawater of above-mentioned lambda sensor 10, when above-mentioned marine organisms oxygen demand deviation do not occur, according to above-mentioned each temperature-fall period, gradually reduce the holding time of ocean temperature of above-mentioned each temperature-fall period.In addition, above-mentioned computer 9 also can be applicable among the various embodiment of the present invention marine organisms be implemented the automaticmanual hibernation.
Regulate ocean temperature and hold time by above-mentioned artificial hibernation apparatus for deivation, thereby finish halobiontic artificial hibernation induced after, the marine organisms that enter artificial hibernation are put into case with external insulation, the lid of getting wet on above-mentioned marine organisms surface cover is kept certain humidity then, will transport after the above-mentioned chest sealing.
If be equipped with in the halobiontic chest and flow into air or heat from the outside, can cause the temperature inside the box to rise, the marine organisms that enter artificial hibernation can revive and death.So among the present invention, in order to keep the artificial hibernation state of stipulated time, but the heat of covering outer or the chest that air flows into have been used.Though the temperature to above-mentioned cabinet interior is not particularly limited, confirmed that 1 ℃ to the 2 ℃ the most suitable marine organisms of internal temperature in the scope keep the artificial hibernation state.
In addition, among the present invention, above-mentioned marine organisms make it enter hibernation by the people for inducing, though do not need water, in order to keep the required humidity of marine organisms existence, have used the lid through immersion.Specifically be that l Water Paper on above-mentioned marine organisms surface cover is kept humidity more than 90% for well.
According to the present invention, on the basis in biorhythm in grasp is halobiontic, induce above-mentioned marine organisms to enter hibernation artificially, thereby only need pack with under the anhydrous state, can keep halobiontic high-servival rate for a long time.
Secondly, pack in the case the above-mentioned marine organisms that enter artificial hibernation and sealing, arrive the destination after, open chest, preferably above-mentioned marine organisms are placed on 7 ℃ to the water of 9 ℃ of temperature.Promptly, the present invention is used to transport the marine organisms that entered artificial hibernation, and is behind the chest transportation marine organisms of use sealing, slightly different according to halobiontic size, for example live fish is put back in 7 ℃ to 9 ℃ the water, and fish will come to life from the artificial hibernation state after the several seconds to several minutes.
As mentioned above, the present invention is an object with the biology in the water, it is different from existing live fish transportation resources fully, specifically be, on the interior basis of grasping biology self,, induce anhydrous state artificially by the variations in temperature of several stages in biorhythm, halobiontic inherent biorhythm is stopped for a long time, make it return to the brand new technical of original biorhythm more then.Below, with reference to accompanying drawing one embodiment of the invention are elaborated.By embodiment described later, can deepen the understanding of the present invention, embodiment described later is used to illustrate the present invention, but technical scheme of the present invention its be not limited to embodiment.
What use in the experiment is that the Jizhou Island of buying on Korea S's peace scenery with hills and waters produce market produces flatfish.The experiment total quantity is 290, wherein has 63 by what existing method used that plastic sack or newspaper packing transport, use the present invention artificial hibernation inductive technology (using anhydrous transportation and packing technology) have 227.
Embodiment 1: respiratory activity of flatfish and inherent biorhythm analysis
At first, in order to understand the artificial hibernation of using halobiontic inherent biorhythm, need analyze the inherent biorhythm that the target fish have.
The requirement for experiment condition of measuring the inherent biorhythm of fish is very high.In this experiment condition, plural at least external environment condition is kept certain state, just can be identified as inherent biorhythm (Palmer 1995).Because of fish have eyes, see that light can influence melanin, action and the physiologically active of fish brought influence, thereby be difficult to observe inherent biorhythm.Among the present invention, but used shading and keep particular water temperature (Fig. 4: 19 ℃, Fig. 6: 21.5 ℃) the BOD incubator (VS 1203P5N, Vison Co., Seoul, Korea).Respiratory activity and its inherent biorhythm analysis to breed flatfish are to measure by the Self-breathing analyzer (Automatic intermittent-flow-respirometer:AIFR) of inventor's exploitation.The respiration monitoring data of using AIFR to measure in continuous 135 hours, by specific program (KaleidaGraphy, Synergy Software, Essex Junction, VT USA), uses method of least squares to represent (Fig. 4, Fig. 6) with 2% rolling average.Used maximum entropy spectrum analysis (Maximum EntropySpectral Analysis:MESA) program in the inherent biorhythm analysis that the respiratory activity of flatfish causes.The cycle analysis of biorhythm needs the continuous data in the specified time interval, in the data of measuring by above-mentioned AIFR, after per ten minutes numerical transformations are mean value, analyze with the MESA program, thereby drawn cycle analysis data as shown in Figure 5.As shown in Figure 7, the flatfish of breed demonstrates 24.8 hours the cycle of approaching 24 circadian rhythm 24s.But wild fish or marine organisms are observed it continuously by AIFR and breathe, and demonstrate distinct result as shown in Figure 6.Fig. 6 uses 260 hours measurement result of AIFR METHOD FOR CONTINUOUS DETERMINATION for the Ruditapes philippinarum (Manila clam, Ruditapes philippinarum) that inhabits the intertidal zone is breathed in BOD incubator (21.5 ℃ of dark state and particular water temperature).With the result of 2% moving average analysis, two minor peaks appearred in one day, and the result by MESA analyzes these data as shown in Figure 7, observes 12.2 hours the morning and evening tides rhythm and pace of moving things that influenced by the moon, also observes 25.1 hours circadian rhythm.As can be known, marine organisms are according to its habitat difference in the inherent biorhythm experiment of above-mentioned Ruditapes philippinarum, and biorhythm is also different.With the halobiontic breathing of the long-term mensuration of AIFR, know its inherent biorhythm, and these data are to induce marine organisms to enter the important basis of artificial hibernation.
Embodiment 2: reduce the average oxygen demand of flatfish that changes according to ocean temperature
According to the flatfish respiratory activity in the foregoing description 1 and inherent biorhythm analytical method and data, use the present invention's artificial hibernation apparatus for deivation, when determining to include halobiontic ocean temperature and progressively reducing, above-mentioned halobiontic oxygen demand changes and obviously reduces or the ocean temperature during no change.
Its result as shown in Figure 8, is reduced to 10 ℃ with original 13 ℃ water temperature, and the existence of flatfish is not affected.Therefore, the inventor keeps above-mentioned ocean temperature 10 minutes at 13 ℃, exposes 20 minutes in 12 ℃, exposes 30 minutes in 11 ℃, has progressively increased open-assembly time along with temperature reduces.In 6 ℃ of water temperatures, kept 120 minutes of 12 times of open-assembly times (10 minutes) when 13 ℃ of beginning temperature, in 5 ℃ 180 minutes, and for reduce as far as possible stress, in 4 ℃ of water temperatures that biorhythm stops, keeping the longest 260 minutes.Then, Yi Bian reduce water temperature, Yi Bian shortened open-assembly time gradually.Specifically be, be exposed in 3 ℃ 180 minutes, in 2 ℃ 120 minutes, in 1 ℃ 20 minutes, in subzero 0.2 ℃, exposed 15 minutes at last.
What Fig. 9 showed is to reduce ocean temperature stage by stage, and progressively increases the example that ocean temperature is held time according to its ocean temperature.It is to automatically adjust by the computer program in the artificial hibernation apparatus for deivation that this water temperature changes.Through result of experiment repeatedly, confirmed that artificial hibernation induces starting point to be set in about 13 ℃, can farthest reduce marine organisms suffered stress, and whole hibernation induction time need be spent about 20 hours.
Based on the average oxygen demand change curve of marine organisms among Fig. 8 with the ocean temperature variation, calculated under certain fiducial temperature, the slope of curve that halobiontic average oxygen demand changes in the oxygen demand maximum of flatfish and the variation amplitude of minimum of a value and oxygen demand, each ocean temperature interval, the result is as shown in table 1.
[table 1: the slope in the oxygen demand amplitude of flatfish and each water temperature interval]
As shown in table 1, when water temperature when 13 ℃ drop to 10 ℃, the slope of the oxygen demand amplitude of flatfish is 1.77.When ocean temperature from 10 ℃ to 8 ℃, when reducing by 2 ℃ respectively from 8 ℃ to 6 ℃, above-mentioned slope is all 0.25, less than the slope when 13 ℃ drop to 10 ℃.Thereby breathing or the metabolic activity of judging flatfish in 10 ℃ to 6 ℃ the water temperature interval do not have much variations.And in 6 ℃ to 4 ℃ temperature range, the slope of oxygen demand amplitude reaches 2.7, illustrates that great changes have taken place the metabolic activity in this water temperature range.When ocean temperature is below 4 ℃ the time, oxygen demand almost keeps particular value (6.1ml O
2Kg
-1WW h
-1), slope is 0.05, oxygen demand did not almost change when water temperature changed.
Embodiment 3: use the artificial hibernation experiment of live fish and flatfish
Secondly, the live fish and the flatfish that enter artificial hibernation are put into the case that its inner constant temperature remains on 5 ℃, behind wet Korea Spro's paper on the whole fish surface cover, chest are sealed keeping.Write down variation with the storage time, live fish and flatfish holding time under anhydrous state in the above-mentioned case, and the survival rate of above-mentioned live fish and flatfish, its result is as shown in table 2.
[table 2: the artificial hibernation experimental result of using live fish and flatfish]
| Experiment | The experiment individual number | Individual weight | Does carrying out each stage water temperature handle? | Use l Water Paper? | Anhydrous state is held time | Survival rate |
| Existing method (not using the artificial hibernation abductive approach) | 63 | 820～1060g | × | × | 12～15 |
20～30％ |
| Artificial hibernation inductive technology and anhydrous transportation and packing technology | 140 | 720～1340g | ○ | ○ | 18～24 hours | 90～100％ |
| Artificial hibernation inductive technology and anhydrous transportation and packing technology | 87 | 2020～2740g | ○ | ○ | 18～24 hours | 90～100％ |
As shown in table 2, compare with existing method, used the live fish and the flatfish of the present invention's artificial hibernation inductive technology and anhydrous transportation and packing technology, its anhydrous state length of holding time, and also the survival rate of live fish and flatfish obviously improves.
So far, describe the present invention by accompanying drawing and desirable embodiment, those skilled in the art can carry out various modifications and changes to the present invention under the situation that does not exceed technical characterictic scope of the present invention.
According to the present invention, can implement artificial hibernation to marine organisms, like this, only need under anhydrous state, the above-mentioned marine organisms that enter hibernation to be put into carton, just can keep halobiontic high-servival rate for a long time.Be different from existing live fish transportation resources, the invention provides a kind of with all organisms in water as object, on the interior basis of grasping biology self in biorhythm, variations in temperature by several stages, induce anhydrous state artificially, make halobiontic inherent biorhythm be in halted state for a long time, make it return to the brand-new technology of original biorhythm more then.
Claims (12)
1. a halobiontic artificial hibernation abductive approach is characterized in that, comprising:
Step 1 is placed marine organisms and is kept special time in seawater;
Step 2 according to reducing above-mentioned ocean temperature between given zone stage by stage, and progressively increases thermostatic hold-time in above-mentioned each temperature-fall period at each temperature-fall period;
Step 3, in above-mentioned each temperature-fall period, the ocean temperature during with above-mentioned halobiontic oxygen demand zero deflection is a starting point, gradually reduces the thermostatic hold-time in each temperature-fall period.
2. a halobiontic artificial hibernation abductive approach is characterized in that, comprising:
Step 1, placement has the marine organisms of the 1st oxygen demand and keeps special time in the seawater with particular water temperature;
Step 2 the interval reduces above-mentioned ocean temperature according to the rules stage by stage, and in above-mentioned each temperature-fall period, keeps this temperature-fall period ocean temperature of special time, and progressively increases the thermostatic hold-time in above-mentioned each temperature-fall period;
Step 3 in above-mentioned each temperature-fall period, is in 1/1.5th to 1/4th scopes of above-mentioned the 1st oxygen demand with above-mentioned halobiontic average oxygen demand, and perhaps the deviation with above-mentioned halobiontic oxygen demand is in 6.0ml O
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1Ocean temperature in the time of in the scope is a starting point, gradually reduces the thermostatic hold-time in above-mentioned each temperature-fall period.
3. a halobiontic artificial hibernation abductive approach is characterized in that, comprising:
Step 1 is in the seawater of the 1st temperature in water temperature, and placement has the marine organisms of the 1st oxygen demand and kept for the 1st time period;
Step 2, progressively reduce above-mentioned ocean temperature, in the 2nd temperature, above-mentioned ocean temperature was kept for the 2nd time period, wherein above-mentioned the 2nd temperature is 1/1.5th to 1/2.5th scopes that above-mentioned halobiontic oxygen demand is in above-mentioned the 1st oxygen demand, temperature when promptly being in the 2nd oxygen demand, and above-mentioned the 2nd time period is equivalent to 10 times to 25 times the time span of above-mentioned the 1st time period;
Step 3, reduce above-mentioned ocean temperature gradually, in the 3rd temperature, above-mentioned ocean temperature was kept for the 3rd time period, wherein above-mentioned the 3rd temperature is 1/3rd to 1/4th scopes that above-mentioned halobiontic oxygen demand is in above-mentioned the 1st oxygen demand, temperature when promptly being in the 3rd oxygen demand, and above-mentioned the 3rd time period is equivalent to 26 times to 100 times the time span of above-mentioned the 1st time period.
4. a halobiontic artificial hibernation abductive approach is characterized in that, comprising:
Step 1, the marine organisms that will have the 1st oxygen demand are placed in the seawater that water temperature is the 1st temperature and kept for the 1st time period;
Step 2 progressively reduces above-mentioned ocean temperature, in the 3rd temperature, above-mentioned ocean temperature was kept for the 3rd time period, wherein above-mentioned the 3rd temperature is, in above-mentioned halobiontic oxygen demand reduces to 1/3rd to 1/4th scopes of above-mentioned the 1st oxygen demand, and the temperature when promptly being in the 3rd oxygen demand; And above-mentioned the 3rd time period is equivalent to the time span in 10 times to the 100 times scopes of above-mentioned the 1st time period.
5. a halobiontic artificial hibernation abductive approach is characterized in that, comprising:
Step 1 is to place marine organisms in the seawater of the 1st temperature and kept for the 1st time period in water temperature;
Step 2 progressively is reduced to the maximum of above-mentioned marine organisms oxygen demand with above-mentioned ocean temperature and the difference of minimum of a value is in 6.0ml O
2Kg
-1WW h
-1To 1.0ml O
2Kg
-1WW h
-1The 2nd temperature in the time of in the scope, and in above-mentioned the 2nd temperature, above-mentioned seawater is kept 10 times of the 2nd time periods to 25 times of scope time spans that are equivalent to above-mentioned the 1st time period;
Step 3 progressively is reduced to the maximum of above-mentioned marine organisms oxygen demand with above-mentioned ocean temperature and the difference of minimum of a value is in 1.1ml O
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1The 3rd temperature in the time of in the scope, and in above-mentioned the 3rd temperature, above-mentioned seawater is kept 26 times of the 3rd time periods to 100 times of scope time spans that are equivalent to above-mentioned the 1st time period.
6. a halobiontic artificial hibernation abductive approach is characterized in that, comprising:
Step 1 is to place marine organisms in the seawater of the 1st temperature and kept for the 1st time period in water temperature;
Step 2 progressively is reduced to the maximum of above-mentioned marine organisms oxygen demand with above-mentioned ocean temperature and the difference of minimum of a value is in 1.1ml O
2Kg
-1WW h
-1To 0.0ml O
2Kg
-1WW h
-1The 3rd temperature in the time of in the scope, and in above-mentioned the 3rd temperature, above-mentioned seawater is kept 10 times of the 3rd time periods to 100 times of scope time spans that are equivalent to above-mentioned the 1st time period.
7. as halobiontic artificial hibernation abductive approach as described in each in the claim 3 to 6, it is characterized in that:
Progressively reduce the temperature of above-mentioned seawater, exactly above-mentioned ocean temperature is reduced in the interval according to the rules stage by stage, and progressively increase thermostatic hold-time in above-mentioned each temperature-fall period at above-mentioned each temperature-fall period.
8. as halobiontic artificial hibernation abductive approach as described in claim 3 or 5, it is characterized in that:
Above-mentioned the 1st temperature is 14 ℃ to 12 ℃, and above-mentioned the 2nd temperature is 7 ℃ to 5 ℃, and above-mentioned the 3rd temperature is 5 ℃ to 3 ℃.
9. as halobiontic artificial hibernation abductive approach as described in claim 4 or 6, it is characterized in that:
Above-mentioned the 1st temperature is 13 ℃, and above-mentioned the 3rd temperature is 4 ℃.
10. as halobiontic artificial hibernation abductive approach as described in each in the claim 1 to 6, it is characterized in that:
Above-mentioned marine organisms are live fish.
11. a halobiontic artificial hibernation apparatus for deivation is characterized in that, comprising:
The artificial hibernation case is equipped with and comprises halobiontic seawater;
Heat exchanger or cooler, the temperature of the seawater that will discharge from above-mentioned artificial hibernation case are back to seawater in the artificial hibernation case after the interval reduces stage by stage according to the rules again;
Measure the temperature sensor of the ocean temperature in the above-mentioned artificial hibernation case, and the lambda sensor that is used to measure above-mentioned dissolved oxygen of seawater tolerance;
Computer, control above-mentioned heat exchanger or cooler, make it according to water temperature information from the said temperature sensor, keep the ocean temperature special time at above-mentioned each temperature-fall period, and progressively increase the thermostatic hold-time of above-mentioned each temperature-fall period, and according to from dissolved oxygen tolerance information in the seawater of above-mentioned lambda sensor, when above-mentioned marine organisms oxygen demand deviation do not occur, gradually reduce the thermostatic hold-time in each temperature-fall period.
12., it is characterized in that as artificial hibernation apparatus for deivation as described in the claim 11:
Above-mentioned artificial hibernation case comprises and is used for salvaging halobiontic fishing net from seawater.
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20060074458 | 2006-08-08 | ||
| KR1020060074458 | 2006-08-08 | ||
| KR10-2006-0074458 | 2006-08-08 | ||
| KR1020070019542 | 2007-02-27 | ||
| KR1020070019544A KR100740457B1 (en) | 2006-08-08 | 2007-02-27 | Method for inducing artificial hibernation of marine animal |
| KR10-2007-0019544 | 2007-02-27 | ||
| KR1020070019542A KR100768475B1 (en) | 2007-02-27 | 2007-02-27 | Apparatus for inducing artificial hibernation of marine animal |
| KR10-2007-0019542 | 2007-02-27 | ||
| KR1020070019544 | 2007-02-27 |
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| CN2007100958650A Expired - Fee Related CN101120664B (en) | 2006-08-08 | 2007-04-10 | Method and apparatus for inducing artificial hibernation of marine animal |
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| Country | Link |
|---|---|
| KR (1) | KR100740457B1 (en) |
| CN (1) | CN101120664B (en) |
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| CN103843719A (en) * | 2012-11-28 | 2014-06-11 | 翟文 | North wood frog living body safety cabin |
| KR101755255B1 (en) | 2015-04-30 | 2017-07-07 | 연세대학교 원주산학협력단 | Method and composition for inducing a long-time hypometabolism and fish transportation method using thereof |
| KR101863586B1 (en) * | 2017-01-10 | 2018-06-05 | 주식회사 더피쉬 | Method for Inducing Artificial Hibernation of Fish, and Container for Packing the Fish |
| US11330805B2 (en) * | 2017-12-27 | 2022-05-17 | The Fish Co., Ltd. | Method for inducing artificial hibernation of fish, live fish packaging method, and live fish packaging container |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5309868A (en) * | 1992-03-24 | 1994-05-10 | Sanki Kogyo Co., Ltd. | Container for live marine animals |
| US5310427A (en) * | 1990-08-03 | 1994-05-10 | Japan Airlines Co., Ltd. | Method for transporting live fish |
| US6557492B1 (en) * | 2002-07-19 | 2003-05-06 | Sea Chick, Inc. | System for transporting and storing live fish, components thereof and methods based thereon |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04287629A (en) * | 1991-03-15 | 1992-10-13 | Calsonic Corp | Operation of water cooler in container for transporting live fishes |
| KR200204965Y1 (en) | 2000-05-27 | 2000-12-01 | 이상철 | A aquarium control system. |
-
2007
- 2007-02-27 KR KR1020070019544A patent/KR100740457B1/en active IP Right Review Request
- 2007-03-06 RU RU2008147036/13A patent/RU2420064C2/en not_active IP Right Cessation
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310427A (en) * | 1990-08-03 | 1994-05-10 | Japan Airlines Co., Ltd. | Method for transporting live fish |
| US5309868A (en) * | 1992-03-24 | 1994-05-10 | Sanki Kogyo Co., Ltd. | Container for live marine animals |
| US6557492B1 (en) * | 2002-07-19 | 2003-05-06 | Sea Chick, Inc. | System for transporting and storing live fish, components thereof and methods based thereon |
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| RU2420064C2 (en) | 2011-06-10 |
| KR100740457B1 (en) | 2007-07-18 |
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| RU2008147036A (en) | 2010-09-20 |
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