CN101770070B - The survey meter of observation of micro object and operational approach - Google Patents
The survey meter of observation of micro object and operational approach Download PDFInfo
- Publication number
- CN101770070B CN101770070B CN200910202141.0A CN200910202141A CN101770070B CN 101770070 B CN101770070 B CN 101770070B CN 200910202141 A CN200910202141 A CN 200910202141A CN 101770070 B CN101770070 B CN 101770070B
- Authority
- CN
- China
- Prior art keywords
- observation
- light transmission
- transmission device
- detected
- survey meter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention provides survey meter and the operational approach of a kind of observation of micro object, belongs to electronic technology field.The implementation method of this equipment includes: step 1, arranges object to be detected in sample placement plate；Step 2, arranges the controlled light transmission device of array, transparent controllable unit contained by the controlled light transmission device of described array, drives the transmittance of transparent controllable unit in the controlled light transmission device of described array, forms transmission region；Step 3, by described transmission region, utilizes optical texture to observe object to be detected, determines observation station；Step 4, by photosensitive structure, the image document of observation station determined by recording step 3.Utilize the controlled light transmission device of the array in the present invention, when detecting object is treated in detection, it is possible to achieve detect one by one, multiple spot comparative observation, the function of observation station location, and be possible to prevent repeated measures, save the observation time of user, it is provided that observed efficiency.
Description
Technical field
The invention belongs to electronic technology field, particularly relate to the survey meter of a kind of observation of micro object
And operational approach.
Background technology
Along with developing rapidly of science and technology, the field of finding out of the mankind is also constantly expanding, only according to
Naked-eye observation cannot meet the desire for knowledge of the mankind, and microscopical invention makes the mankind to microcosmic
There has been the most deep understanding in the world, and these knowledge obtained instruct people to lead in science
Territory, medical domain are constantly kept forging ahead development.
And going deep into along with the field of finding out, the object needing observation is more and more less, if it is desired to micro-
Little object is observed, and when being such as observed cell, often can face a subject matter,
That object being just because being observed is the most small, and utilizing is with the amplification observation of high power
Time, often can be faced with the object of observation that quantity is the hugest.
How the object of observation of these astronomical number is effectively observed, be one and be not easy to
The problem solved, but meaning is the biggest.In actual application, such as, thin to substantial amounts of body
When born of the same parents observe, in order to retrieve possible sick cell, it is obviously desirable to object to be detected
Carry out substantial amounts of observation.The most not only take time and effort, and observe because cannot accurately distinguish
Region and be observation area, thus cause the repeatability of observation, and for fixed multiple sights
When measuring point is shown one by one, because there is no good observing the most one by one with reference to a little needs.
Summary of the invention
The present invention provides survey meter and the operational approach of a kind of observation of micro object, utilizes the present invention
In the controlled light transmission device of array, when detecting object is treated in detection, it is possible to achieve visit one by one
Survey, prevent from repeating detection, the function of observation station location.
It is an object of the invention to provide the survey meter of a kind of observation of micro object, include:
Sample placement plate, it is the sample stage placing object to be detected；
The controlled light transmission device of array, it is made up of substantial amounts of transparent controllable unit, and each
Transparent controllable unit, its light transmission features all can obtain the structure controlled；
Optical texture, it is used to carry out optical amplifier, the structure of optical image-taking；
Photosensitive structure, it is to match with aforesaid optical texture, in order to through optical texture
Object under test image after process carries out the structure of photosensitive record.
Further, described survey meter also includes:
Described survey meter is provided with lighting source, and it is for being placed on described sample placement plate
On object to be detected provide illumination structure；Described lighting source includes with bottom as angle
Degree, in order to provide the soffit lighting light source of illumination to object to be observed, and with sidepiece as angle,
In order to provide the sidepiece lighting source of illumination to object to be observed.
Described sample placement plate is light-passing board, and it is for allowing above-mentioned soffit lighting light source directly wear
Thoroughly, the sample stage of object to be detected is irradiated.
The controlled light transmission device of described array is liquid crystal matrix layer, and it is can for each printing opacity
Control unit, is correspondingly arranged on a liquid crystal layer unit, controls described liquid crystal layer unit by power supply
The structure of light transmission.
The controlled light transmission device of described array is provided with transmission region control module, and it is for controlling
Make described transparent controllable unit and form transmission region, transmission region scope, and plan transmission region
The structure of course.
The controlled light transmission device of described array is provided with coordinate respective modules, and it is for working as user
When determining the observation station of object to be detected, record transparent controllable unit corresponding to described observation station
The structure of coordinate position.
The controlled light transmission device of described array be the polygonal printing opacity of geometry by least one can
The structure of control unit composition.
It is a further object of the present invention to provide the operation side of the survey meter of a kind of observation of micro object
Method, includes:
Step 1, arranges object to be detected in sample placement plate；
Step 2, arranges the controlled light transmission device of array, described array controlled light transmission device institute
Containing transparent controllable unit, drive the saturating of transparent controllable unit in the controlled light transmission device of described array
Luminosity, forms transmission region；
Step 3, by described transmission region, utilizes optical texture to observe object to be detected, really
Determine observation station；
Step 4, by photosensitive structure, the image money of observation station determined by recording step 3
Material.
Further, the operational approach of described survey meter also includes:
In step 1, described band detecting object is placed in having the sample of lighting source illumination and puts
Put on plate.
In step 2, described transmission region be turned on or off route for voluntarily or in advance
Set.
It is an advantage of the current invention that: the survey meter of the observation of micro object utilizing the present invention to provide,
Correspond to object to be detected, arrange the controlled light transmission device of array, this device includes substantial amounts of
Transparent controllable unit.When observing, can only one or one group of transparent controllable unit be changed every time
Becoming light transmission state, forming transmission region other parts is light tight state, forms shaded areas.
Then, the part-structure independence imaging of the object to be detected presented facing to this transmission region.Then
Convert other transparent controllable unit again, form new light transmission state, continue to observe.Former with this
Reason, carries out image observation to the object to be detected of substantial amounts, and in observation process not
There will be the situation of repeated measures, need to observe or multiple sights of comparison for having determined that simultaneously
Measuring point, can control the controlled light transmission device of array, arrange the plurality of observation station simultaneously
Light region, also can the transparent controllable unit coordinate position of the plurality of observation station of labelling, at optics
Structure or photosensitive structure fill, is shown to user.
Accompanying drawing explanation
The present invention will be described in more detail below in conjunction with the accompanying drawings.
Fig. 1 is the equipment primary structure figure of the survey meter of observation of micro object of the present invention.
Fig. 2 is the flow process of the operational approach of the survey meter of observation of micro object of the present invention
Figure.
Fig. 3 is the operation chart of the survey meter of observation of micro object of the present invention, is one
Plant embodiment.
Fig. 4 is the operation chart of the survey meter of observation of micro object of the present invention, is one
Plant embodiment.
Fig. 5 be observation of micro object of the present invention survey meter in the controlled printing opacity of array
The transmission region of device be turned on and off route schematic diagram.
Fig. 6 be observation of micro object of the present invention survey meter in the controlled printing opacity of array
The transmission region of device zooms in or out schematic diagram.
Fig. 7 be observation of micro object of the present invention survey meter in the controlled printing opacity of array
The schematic shapes of the controlled light transmitting cells of device.
Detailed description of the invention
Referring to accompanying drawing, in conjunction with specific embodiment, the present invention is described further.
First, shown in ginseng Fig. 1, the overall structure of the present invention is explained.
Shown in ginseng Fig. 1, this figure illustrates the survey meter 100 of this observation of micro object
Primary structure, including sample placement plate 210, it is the sample stage placing object to be detected,
Can be provided for being placed on described sample as required around described sample placement plate 210
Object to be detected in placement plate provides the lighting source 250 of illumination, including with bottom as angle,
In order to provide the soffit lighting light source 251 of illumination to object to be observed, and with sidepiece as angle,
In order to provide the sidepiece lighting source 252 of illumination to object to be observed, and described sample is put
Plate 210 can be made by transparent material, allows soffit lighting light source 251 can be directed through sample
Placement plate 210, is radiated on object to be detected.
Described survey meter 100 is additionally provided with the controlled light transmission device of array 220, and it is by substantial amounts of
Transparent controllable unit 221 forms, and each transparent controllable unit 221, and its light transmission features is equal
Can be controlled, concrete, the controlled light transmission device of described array 220 can use liquid crystal
Layer realizes.Each transparent controllable unit 221, is correspondingly arranged on a liquid crystal layer unit,
It is similar to a pixel, and the profile of transparent controllable unit 221 can be equilateral triangle, square
Shape, regular hexagon, or other one or more geometric combination.
And by transmission region control module 222, described transparent controllable unit 221 can be controlled
Form transmission region, transmission region scope, and plan transmission region course, concrete,
Described transmission region can have the combination of multiple transparent controllable unit 221 to be formed, it is also possible to the same to time-division
It is dispersed on the controlled light transmission device of array 220.The printing opacity of described transparent controllable unit 221 or
Covering route can be by transmission region control module 222, such as scan-type one by one, or Z-shaped
Sampling observation formula.And for the range size of transmission region, can be multiple adjacent by controlling simultaneously
Transparent controllable unit 221 printing opacity simultaneously expands transparency range, or reduces a certain transparent controllable list
The glazed area of unit 221 reduces transparency range.For each transparent controllable unit 221,
Can arrange coordinate position by coordinate respective modules 223, user is determining object to be detected
Observation station time, the printing opacity that described observation station is corresponding can be recorded by coordinate respective modules 223
The coordinate position of controllable 221, user finds described sight again on described object to be detected
During measuring point, by opening the transparent controllable unit 221 corresponding with record coordinate, it is possible to quickly
Location.
Described survey meter 100 is additionally provided with for carrying out optical amplifier, the optics knot of optical image-taking
Structure 230, and match, in order to through optical texture 230 with aforesaid optical texture 230
Object under test image after process carries out the photosensitive structure 240 of photosensitive record, passes through optical texture
230 and photosensitive structure 240, such as microscope and electronic imaging system, it is possible to detected
Object is amplified observation, or imaging processing.
Explanation is launched below in conjunction with some specific embodiments.
The explanation of Fig. 3, Fig. 4:
Fig. 3 is the operation chart of the survey meter of observation of micro object of the present invention, is one
Plant embodiment.
Fig. 4 is the operation chart of the survey meter of observation of micro object of the present invention, is one
Plant embodiment.
As it is shown on figure 3, in sample placement plate 210-1 of the survey meter 100 of observation of micro object
Article 310-1 and 310-2 to be detected placed.Described sample placement plate 210-1 is light-passing board,
Carry out light source by soffit lighting light source 251-1 and sidepiece lighting source 252-2 to supplement.
Now user observes article to be detected by optical texture 230-1 and photosensitive structure 240-1
310-1 and 310-2.
If it is according to common observation procedure, the most micro-at article 310-1 and 310-2 to be detected
The when of little, in the longest observation time, user does not observes thing, easily upsets user
Observation order.
And by the array controlled light transmission device 220-1 of survey meter 100, user just can be led to
Cross control array controlled light transmission device 220-1 transparent controllable unit 221-1 printing opacity or
Screening effect, observes one by one to the carrier in sample placement plate 210-1.
As described in Figure 3, user controls array controlled light transmission device 220-1, and opening printing opacity can
Control unit 2211, when forming transmission region 224-1, has observed object 310-1 to be detected.
And as described in Figure 4, user continues to control array controlled light transmission device 220-2, open
Transparent controllable unit 221-2, when forming transmission region 224-2, has observed object to be detected
310-2。
User can be logical when observing object 310-1 to be detected and object 310-2 to be detected
Cross survey meter 100-1, record corresponding to transmission region 224-1 and transmission region 224-2
Transparent controllable unit 221-1 and the coordinate position of transparent controllable unit 221-2.When user again
When needing to observe above-mentioned object 310-1 to be detected and object 310-2 to be detected, or carry out
During comparative observation, the coordinate position that can be recorded by input, control array light transmission device
220-1 (2), opens transparent controllable unit 221-1 and transparent controllable unit 221-2 simultaneously,
Again observe.
The explanation of Fig. 5:
Fig. 5 be observation of micro object of the present invention survey meter in the controlled printing opacity of array
The transmission region of device be turned on and off route schematic diagram.
As shown in fig. 5-1, in user's setting array formula controlled light transmission device 220-31, printing opacity can
The opening process that printing opacity process is scan-type one by one of control unit 221-31, it is adaptable to observation is seen
Measuring point ratio is relatively decentralized, and each band detecting object having characteristic.
As shown in Fig. 5-2, in user's setting array formula controlled light transmission device 220-32, printing opacity can
The printing opacity process of control unit 221-32 is the opening process of Z-shaped sampling observation formula, it is adaptable to observation
Observation station compares concentration, has the band detecting object of general character.
The explanation of Fig. 6:
Fig. 6 be observation of micro object of the present invention survey meter in the controlled printing opacity of array
The transmission region of device zooms in or out schematic diagram.
As in Figure 6-1, in user's setting array formula controlled light transmission device 220-41, printing opacity can
Control unit 221-41 is the many reopenings of non-conterminous cell, forms multiple transmission region 224-41
It is easy to user and observes the band detecting object with multiple observation station simultaneously.
As in fig. 6-2, in user's setting array formula controlled light transmission device 220-42, printing opacity can
Control unit 221-42 is the many reopenings of adjacent cells lattice, forms bigger transmission region 224-42 just
The band detecting object with bigger observation station is seen in user.
As shown in Fig. 6-3, in user's setting array formula controlled light transmission device 220-43, printing opacity can
Control unit 221-43 reduces unlatching for single lattice, forms less transmission region 224-43 and is easy to user
See the band detecting object with less observation station.
The explanation of Fig. 7:
Fig. 7 be observation of micro object of the present invention survey meter in the controlled printing opacity of array
The schematic shapes of the controlled light transmitting cells of device.
Transparent controllable as shown in Fig. 7-1, in described array controlled light transmission device 220-51
The profile of unit 221-51 is equilateral triangle, described array controlled light transmission device 220-51 by
Multiple equilateral triangles repeat built-up.
Transparent controllable as shown in Fig. 7-2, in described array controlled light transmission device 220-52
The profile of unit 221-52 is regular hexagon, described array controlled light transmission device 220-52 by
Multiple regular hexagons repeat built-up.
Transparent controllable as shown in Fig. 7-3, in described array controlled light transmission device 220-53
The profile of unit 221-531 is dodecagon, and the profile of transparent controllable unit 221-531 is
Equilateral triangle, described array controlled light transmission device 220-53 is by multiple dodecagons and many
Individual equilateral triangle repeats built-up.
The explanation of Fig. 2:
Step 1, arranges object to be detected in sample placement plate；
As it is shown on figure 3, place object 310-1 to be detected in described sample placement plate 210-1
And object 310-2 to be detected.
Step 2, arranges the controlled light transmission device of array, described array controlled light transmission device institute
Containing transparent controllable unit, drive the saturating of transparent controllable unit in the controlled light transmission device of described array
Luminosity, forms transmission region；
As it is shown in figure 5, the open mode that can arrange transmission region is scan-type one by one, or
Z-shaped sampling observation formula.
As shown in Figure 6, the number of transmission region can be set, the size of transparency range.
Step 3, by described transmission region, utilizes optical texture to observe object to be detected, really
Determine observation station；
As it is shown on figure 3, user has observed object to be detected in transmission region 224-1
310-1, and as shown in Figure 4, user has observed thing to be detected in transmission region 224-2
Body 310-2.
Step 4, by photosensitive structure, the image money of observation station determined by recording step 3
Material.
It is above the description of this invention and non-limiting, other embodiment party based on inventive concept
Formula, all among protection scope of the present invention.
Claims (9)
1. the survey meter of an observation of micro object, it is characterised in that the survey meter of described observation of micro object is provided with
Sample placement plate, it is the sample stage placing object to be detected；
The controlled light transmission device of array, it is made up of substantial amounts of transparent controllable unit, and each transparent controllable unit, and its light transmission features all can obtain the structure controlled, and transparent controllable unit described in a state of use is seated in above aforementioned object to be detected；
Optical texture, it is used to carry out optical amplifier, the structure of optical image-taking；
Photosensitive structure, it is to match with aforesaid optical texture, in order to the object under test image after optical texture processes to be carried out the structure of photosensitive record,
Wherein, correspond to aforesaid object to be detected and lighting source is set, provide illumination for angle to object to be observed with bottom or sidepiece, and the aforesaid optical texture with optical amplifier effect is arranged between aforesaid photosensitive structure and transparent controllable unit.
2. according to the survey meter of a kind of observation of micro object described in claim 1, it is characterised in that: described sample placement plate is light-passing board, and it is for allowing above-mentioned soffit lighting light source be directed through, irradiating the sample stage of object to be detected.
3. according to the survey meter of a kind of observation of micro object described in claim 1, it is characterized in that: the controlled light transmission device of described array is liquid crystal matrix layer, it is for each transparent controllable unit, it is correspondingly arranged on a liquid crystal layer unit, is controlled the structure of the light transmission of described liquid crystal layer unit by power supply.
4. according to the survey meter of a kind of observation of micro object described in claim 1 or 3, it is characterized in that: the controlled light transmission device of described array is provided with transmission region control module, it is for controlling described transparent controllable unit formation transmission region, transmission region scope, and plan the structure of transmission region course.
5. according to the survey meter of a kind of observation of micro object described in claim 1 or 3, it is characterized in that: the controlled light transmission device of described array is provided with coordinate respective modules, when it is for determining the observation station of object to be detected as user, record the structure of the coordinate position of transparent controllable unit corresponding to described observation station.
6. according to the survey meter of a kind of observation of micro object described in claim 1 or 3, it is characterised in that: the controlled light transmission device of described array is by the structure that at least one is that geometry polygonal transparent controllable unit forms.
7. implement the operational approach of the survey meter of observation of micro object described in claim 1 for one kind, it is characterised in that the operational approach of the survey meter of described observation of micro object includes:
Step 1, arranges object to be detected in sample placement plate；
Step 2, the controlled light transmission device of array is set, transparent controllable unit described in a state of use is seated in above aforementioned object to be detected, transparent controllable unit contained by the controlled light transmission device of described array, drive the transmittance of transparent controllable unit in the controlled light transmission device of described array, form transmission region；
Step 3, by described transmission region, utilizes optical texture to observe object to be detected, determines observation station；
Step 4, by photosensitive structure, the image document of observation station determined by recording step 3.
8. according to the operational approach of the survey meter of a kind of observation of micro object described in claim 7, it is characterised in that: in step 1, described object to be detected is placed in be had in the sample placement plate of lighting source illumination.
9. according to the operational approach of the survey meter of a kind of observation of micro object described in claim 7, it is characterised in that: in step 2, described transmission region be turned on or off route for voluntarily or set in advance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910202141.0A CN101770070B (en) | 2009-12-31 | 2009-12-31 | The survey meter of observation of micro object and operational approach |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910202141.0A CN101770070B (en) | 2009-12-31 | 2009-12-31 | The survey meter of observation of micro object and operational approach |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101770070A CN101770070A (en) | 2010-07-07 |
| CN101770070B true CN101770070B (en) | 2016-09-07 |
Family
ID=42503034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910202141.0A Active CN101770070B (en) | 2009-12-31 | 2009-12-31 | The survey meter of observation of micro object and operational approach |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101770070B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5065008A (en) * | 1989-10-18 | 1991-11-12 | Fuji Photo Film Co., Ltd. | Scanning microscope and scanning mechanism for the same |
| CN1635621A (en) * | 2003-12-30 | 2005-07-06 | 中芯国际集成电路制造(上海)有限公司 | Locating structure and locating method thereof |
| CN101305275A (en) * | 2005-11-30 | 2008-11-12 | 株式会社尼康 | Observing device |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03134608A (en) * | 1989-10-20 | 1991-06-07 | Fuji Photo Film Co Ltd | Scanning type microscope |
| JPH05210051A (en) * | 1992-01-30 | 1993-08-20 | Yokogawa Electric Corp | Confocal optical scanner |
| JPH10293255A (en) * | 1997-04-22 | 1998-11-04 | Sanyo Electric Co Ltd | Optical microscope |
| JP2004069373A (en) * | 2002-08-02 | 2004-03-04 | Japan Science & Technology Corp | Liquid crystal precision position controller |
| JP3754958B2 (en) * | 2002-12-25 | 2006-03-15 | 独立行政法人科学技術振興機構 | High magnification microscope |
| JP2007085978A (en) * | 2005-09-26 | 2007-04-05 | Shimadzu Corp | Microspectro system |
-
2009
- 2009-12-31 CN CN200910202141.0A patent/CN101770070B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5065008A (en) * | 1989-10-18 | 1991-11-12 | Fuji Photo Film Co., Ltd. | Scanning microscope and scanning mechanism for the same |
| CN1635621A (en) * | 2003-12-30 | 2005-07-06 | 中芯国际集成电路制造(上海)有限公司 | Locating structure and locating method thereof |
| CN101305275A (en) * | 2005-11-30 | 2008-11-12 | 株式会社尼康 | Observing device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101770070A (en) | 2010-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11947096B2 (en) | Microscopy imaging | |
| Lapp et al. | A new luminescence detection and stimulation head for the Risø TL/OSL reader | |
| US6287852B1 (en) | Photosynthetic culture apparatus and group of photosynthesis culture apparatuses | |
| Kaye et al. | Laser-stimulated fluorescence in paleontology | |
| US7630065B2 (en) | Low-light specimen image pickup unit and low-light specimen image pickup apparatus | |
| JP6134422B2 (en) | Stem cell observation method and stem cell observation apparatus | |
| AU2002347379A1 (en) | Device and method for investigating analytes in liquid suspension or solution | |
| EP1754257A2 (en) | Optical lens system and method for microfluidic devices | |
| US9488598B2 (en) | Inspecting apparatus for inspecting a multilayer structure | |
| CN101498721B (en) | Colloidal-gold strip measuring method and detection system | |
| CN104849250B (en) | Portable intelligent plant physiology condition detecting system and method | |
| CN101770070B (en) | The survey meter of observation of micro object and operational approach | |
| CN103592267B (en) | Optical correction device, biological detection correction system and operation method thereof | |
| JP2010506164A (en) | Method and system for detection by forward illumination | |
| CN103620376A (en) | Method for observing sample | |
| CN102446784A (en) | Testing method and apparatus of polycrystalline silicon thin film | |
| CN101893809B (en) | Light source device for fluorescence photography | |
| CN207650107U (en) | Illuminate monitoring system and vegetation monitoring device | |
| CN205538988U (en) | Energy -conserving ELIASA | |
| CN211856367U (en) | Detector compatible with colorimetric analysis and fluorescence analysis | |
| US11921102B2 (en) | Compact optical imaging system for cell culture monitoring | |
| US20120001095A1 (en) | Light source apparatus for fluorescence photography | |
| JP2011164092A (en) | Method for observation of measuring device for measuring bioluminescence, chemiluminescence, or fluorescence of object, irradiation device, measuring system, and plant | |
| CN213951220U (en) | Optogenetics experimental device based on auxiliary high-flux LED illumination array | |
| WO2022024768A1 (en) | Sample observation device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |