CN101812171A - Conductive polymer for biosensor and preparation method thereof - Google Patents
Conductive polymer for biosensor and preparation method thereof Download PDFInfo
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- CN101812171A CN101812171A CN 201010146022 CN201010146022A CN101812171A CN 101812171 A CN101812171 A CN 101812171A CN 201010146022 CN201010146022 CN 201010146022 CN 201010146022 A CN201010146022 A CN 201010146022A CN 101812171 A CN101812171 A CN 101812171A
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Abstract
The invention provides a conductive polymer for a biosensor and a preparation method thereof in order to solve the problem puzzling the academia for long time about how to combine a bioactive unit to a metal non-inactively and permanently and that bioactive molecules are connected to the conductive polymer in one step. The bioactive unit is first introduced into a conductive polymer monomer, and then the conductive polymer with bioactivity is directly formed in situ on the surface of an electrode in one step by adopting an in-situ electrochemical combinatorial synthesis method and an independent addressing microelectrode array technique so as to obtain a functional microelectrode array applied to the biosensor. The conductive polymer for the biosensor and the preparation method make the development of the biosensor towards the direction of intensification, microminiaturization and micro power consumption become possible, can promote the development of disease diagnosis, medicament screening, health care, judicial expertise, food detection, environmental monitoring, individualized medicament and the like and industrial chains thereof, and have far-reaching social meaning and broad market value.
Description
Technical field
The present invention relates to a kind of conductive polymers that is used for biosensor and preparation method thereof, specifically, relate to synthetic (combinatorial synthesis) method of a kind of employing electrochemical in-situ combination, make to have the biological activity unit (as DNA, RNA, peptide, protein, enzyme, adaptive thing (aptamer), tissue, cell etc.) conductive polymers one step is directly fixed on the electrode, obtains the functionalization microelectrode array to be applied to biosensor.
Background technology
Biosensor is a new and high technology that is interpenetrated and grown up by multiple subjects such as information biology, biological cybernetics, bionics, biocomputer, biology, chemistry, physics, medical science, electronic technology, is a kind of quick, parallel, little quantized analytical procedure of material being carried out molecular level.The test substance biosensor of flowing through, through molecular recognition, the signal that cross reaction between the biological activity unit is expressed is converted into through physics or chemical transverter can quantitative and accessible electrical signal, and carries out informationization and digitizing with computer.Biosensor technology is the new growth point between information and biotechnology, development and bio-science along with Internet of Things, the promotion of information science and Materials science development, and photoconduction, optical fiber, superconduction, nanotechnology, The application of new technique such as intelligent material, the further collection and the transmission of realization information, handle integrated, intelligent, particularly molecular biology and microtronics, photoelectronics, new subject such as Micrometer-Nanometer Processing Technology and nanotechnology, the new technology combination is just changing traditional medicine, health care, medical diagnosis on disease, food inspection, the looks of environmental monitoring.Biosensor is that the perfection of biotechnology and information technology is merged, it is the materialization of bioinformation, be the strategic forward position of Internet of Things, grasped biosensor technology and, just equaled to have captured a strategic high ground of Internet of Things it digitizing, automatization, microminiaturization, intensification, intellectuality.Study on Biosensor is developed, and has become the new focus of development in science and technology of world, forms the important component part of 21 century rising high tech industry, has the important strategic meaning.
Conductive polymers has broad application prospects in fields such as optics, electromagnetism and chemistry because of its special structure and excellent physical chemistry.In recent years, conductive polymers has been obtained certain achievement in research in the application of technical field of biological material.The application of conductive polymers in technical field of biological material is to see as biosensor more.Generally, biologically active substances such as different enzymes, coenzyme, antibody, DNA even cell and tissue are fixed in the conductive polymers, form various novel biosensors.This transmitter has characteristics such as response performance is strong, and making processes is simple, controlled.In making up conductive polymers biosensor process, electrochemical method simple to operate, that be easy to control is adopted in synthesizing of polymkeric substance usually.In polymerization process, biologically active substance both can be aggregated to electrode surface simultaneously with polymer monomer, also can be earlier and monomer whose be adsorbed on electrode surface, reoxidize polymerization and form the bio-identification solid state device.Therebetween, by controlling polymers electropolymerization process effectively, biologically active substance can be fixed to the privileged site of various types of electrodes or electrode, also biologically active substances such as two or more enzymes can be fixed in same layers of polymer film simultaneously or be individually fixed on the multilayer polymeric film.Conductive polymers is as molecular wire, and its 3-D solid structure can make electronics directly transmit at biomolecules (active centre) and electrode surface, significantly improves the response characteristic of biosensor.Spatial distribution in film of thickness by the controlled polymerization film, biomolecules, the indexs such as voidage of polymeric membrane can be adjusted the response characteristic and the selectivity of biosensor.
The key that makes up the conductive polymers biosensor is how biologically active substance to be fixed on the conductive polymerized film effectively, and keeps its biological activity to greatest extent, the bio-identification solid state device that formation can prolonged and repeated effect.The fixing means that biologically active substance is commonly used mainly contains entrapping method, covalent method, absorption method and crosslinking.Wherein see that so that entrapping method more this method depends on electrochemical polymerization and realizes, that is, in electrochemical polymerization, biologically active substance is fixed in the conductive polymerized film with the form of embedding.At present, though the conductive polymers Study on Biosensor has obtained certain achievement, some problems have also been faced simultaneously.But as do not have the conductive polymers biosensor that conventional equipment can be made stable duplication of production, this has limited the widespread use of this type of transmitter at analysis field to a great extent.
Summary of the invention
The present invention for solve puzzlement academia for a long time how with non-inactivation ground, biological activity unit and be permanently attached on the metal and can also settle at one go bioactive molecules is inserted the difficult problem of conductive polymers, a kind of conductive polymers that is used for biosensor and preparation method thereof is provided, has made intensification, microminiaturization and little power consumption of biosensor become reality.
For achieving the above object, the concrete technical scheme of the present invention's employing is as follows:
The conductive polymers that is used for biosensor of the present invention has following general formula:
R=S in the general formula or NH;
R1 in the general formula is
N wherein can be 1,2 or 3; X represents the biological activity unit, can be DNA, RNA, peptide, protein, enzyme, coenzyme, antibody, tissue or cell, preferred vitamin H-vitamin H (Biotin);
R2 in the general formula can be a hydrogen, also can be saturated or unsaturated hydro carbons substituting group, aromatic series (comprising heterocycle) class substituting group, hydro carbons and/or aromatic series (comprising heterocycle) ether or thioether class substituting group, halogen substituting group, through the one-level of protected silane or secondary alcohols substituting group, through the carboxylic acid esters substituting group of tertiary amine, acid amides or the sulfonamides substituting group of amine substituting group, symmetry or the asymmetric replacement of FMOC or BOC protection, saturated or unsaturated hydrocarbons replacement.
The preparation method who is used for the conductive polymers of biosensor of the present invention comprises following concrete steps:
A) preparation has the unitary conductive polymers monomer of biological activity
To have the unitary glycol phosphoramidite of biological activity is dissolved in the anhydrous acetonitrile, add the monomeric anhydrous acetonitrile of conductive polymers then, wherein: having unitary glycol phosphoramidite of biological activity and the monomeric mol ratio of conductive polymers is 10: 1～1: 10; Add an amount of 5-ethylmercapto group tetrazole (ETT) activator (activator), the concentration that makes the activator anhydrous acetonitrile of formation is 0.25mol/L; After at room temperature stirring 20～40 minutes, carry out reduced pressure distillation to Powdered; Add excessive oxidation solution (oxidationsolution), described oxidizing solution is to be dissolved in tetrahydrofuran (THF) by iodine: water: form in the mixed solvent of triethylamine=8: 1: 1, the mass percent concentration of solution is 4%; After stirring 5～15 minutes under the room temperature, be reduced to neutrality with Sulfothiorine, then the decompression steam tetrahydrofuran (THF), with chloroform extraction, organic layer behind anhydrous sodium sulfate drying, the evaporated under reduced pressure organic solvent; Add 10% ammonium hydroxide deprotection at last, be neutralized to neutrality with 0.2M hydrochloric acid after decompression steams excessive ammonia, it is standby to put into-4 ℃ of refrigerators;
B) microelectrode array is based upon on the logic circuit chip of independent addressing, each electrode on this circuit is connected by the transistorized switch of complementary metal oxide semiconductor (CMOS), by sending the electronic address signal to the common junction circuit and then to SRAM (SRAM) conducting this switch relevant with each electrode; Microelectrode array is placed in the fluid reactor, and the reaction solution in the reactor is 1～10 times the not modified conductive polymers monomer that has the unitary conductive polymers monomer of biological activity and its molar weight for preparing of step a) and mixes as electrolytical Repone K in right amount; Open with 10 milliseconds, the pulse of 10 milliseconds of passes, at 0.5～1.5 volt, the logical circuit control of 30 second total time down, the conductive polymers of biologically active generates rapidly in the electrode surface original position immediately, dries up with nitrogen after washed with de-ionized water and promptly gets the functionalization microelectrode array that is used for biosensor.
The described unitary glycol phosphoramidite of biological activity that has is meant and has the unitary glycol phosphoramidite of DNA, RNA, peptide, protein, enzyme, coenzyme, antibody, tissue or cell, preferred vitamin H glycol phosphoramidite.
Described conductive polymers monomer can be that general formula is:
Pyrrole derivative, n wherein can be 1,2 or 3; R2 wherein can be a hydrogen, also can be saturated or unsaturated hydro carbons substituting group, aromatic series (comprising heterocycle) class substituting group, hydro carbons and/or aromatic series (comprising heterocycle) ether or thioether class substituting group, halogen substituting group, through the one-level of protected silane or secondary alcohols substituting group, through the carboxylic acid esters substituting group of tertiary amine, acid amides or the sulfonamides substituting group of amine substituting group, symmetry or the asymmetric replacement of FMOC or BOC protection, saturated or unsaturated hydrocarbons replacement.
Described conductive polymers monomer can also be that general formula is:
Thiophene derivant, n wherein can be 1,2 or 3; R2 wherein can be a hydrogen, also can be saturated or unsaturated hydro carbons substituting group, aromatic series (comprising heterocycle) class substituting group, hydro carbons and/or aromatic series (comprising heterocycle) ether or thioether class substituting group, halogen substituting group, through the one-level of protected silane or secondary alcohols substituting group, through the carboxylic acid esters substituting group of tertiary amine, acid amides or the sulfonamides substituting group of amine substituting group, symmetry or the asymmetric replacement of FMOC or BOC protection, saturated or unsaturated hydrocarbons replacement.
Described electrode can be metal, stainless steel, metal alloy, carbon nanotube, vitreous carbon, reticulated vitreous carbon, graphite, doping oxide, indium tin oxide, silicon oxide, gallium arsenide semiconductor, metal-doped polymkeric substance or stupalith, preferable alloy.
Described metal can be selected from platinum, iridium, palladium, gold and silver, copper, mercury, nickel, zinc, titanium, tungsten or aluminium, preferred platinum.
Just because of each electrode all is connected with independent addressing logic and amplifying circuit under it, so all have intelligent sensing and signal enlarging function, when scanning each electrode with certain pulse wave, can produce a small dipole polarization electronic signal, this signal is slightly variant because of the variation of the residing coenocorrelation of electrode (having or not the molecular recognition reaction), therefore, the utilization of CMOS provides unprecedented repeatability, the ability of synthetic polymer microarray is become a reality bio-sensing in the micron of high flexible and the sub-micrometer range.
The inventor also discovers: along with the carrying out of reaction, the polymer molecule of biologically active and conduction constantly extends when forming current path, and from the nano particle to the nanofiber, last fiber and fiber connect into the sheet network structure successively.By regulation and control monomer concentration, current density and reaction times isoreactivity radical polymerization condition, the polymer growth that can control biologically active and conduction becomes nanofiber.Because nanometer fibrous conductive polymers has the three-dimensional microcosmic structure of nanometer, have unique chemical, physical properties, therefore, can significantly improve detection sensitivity to biomolecules or cell, shorten the reaction times of detecting greatly, make biosensor develop into possibility to intensification, microminiaturization and little power consumption direction.
Compared with prior art, breakthrough point of the present invention mainly contain following some:
1, by earlier the biological activity unit being introduced in the conductive polymers monomer, adopt electrochemical in-situ combination synthetic method then, can one the step directly form the conductive polymers of biologically active in position, solved puzzlement academia for a long time how with non-inactivation ground, biological activity unit and be permanently attached on the electrode and can also settle at one go bioactive molecules is inserted the difficult problem of conductive polymers.
2, by regulation and control monomer concentration, current density and reaction times isoreactivity radical polymerization condition, can control conductive polymers grows into nanometer fibrous, thereby significantly improve detection sensitivity to biomolecules or cell, shorten the reaction times of detecting greatly, make biosensor develop into possibility to intensification, microminiaturization and little power consumption direction.
3, because biosensor of the present invention is as probe with conductive-nano-fibers, have highly sensitive, sampling quantity is little, favorable reproducibility, speed fast, high-throughput, microminiaturization, little power consumption, digitisation, be easy to carry and advantage such as execute-in-place, with the interaction of Internet of Things under, can realize the monitoring of public place epidemic infectious diseases, reach the purpose that prevents trouble before it happens.Therefore, the present invention can promote the development of medical diagnosis on disease, drug screening, health care, judicial expertise, food inspection, environmental monitoring, personalized medicine etc. and above industrial chain, has far-reaching social effect and vast market and is worth.
Description of drawings
Fig. 1 is the structural representation of the independent addressing logic chip among the present invention; Among the figure: 1 is meant the polyreaction driving circuit; 2 are meant the electrode that connects conducting by inner addressing; 3 are meant the electrode that connects by inner addressing but have a mind to no power in experiments and keep inertia;
Fig. 2 is the photo of the prepared functionalization microelectrode array of embodiment 3 under the light of chamber (electrode of 16 micron diameters amplify through 100 times of optics obtain);
Fig. 3 is the photo of the prepared functionalization microelectrode array of embodiment 4 under the light of chamber (electrode of 16 micron diameters amplify through 50 times of optics obtain).
Embodiment
The present invention is described in further detail and completely below in conjunction with embodiment; Used chemical reagent is all to the buying of U.S. Sigma-Aldrich company among the embodiment, and the activator (activator) and the oxidizing solution (oxidation solution) of the usefulness that vitamin H glycol phosphoramidite and confession DNA are synthetic are to purchase from U.S. Glen Research company.
Embodiment 1: preparation has the unitary pyrrole derivative monomer of biological activity
Vitamin H glycol phosphoramidite is dissolved in the anhydrous acetonitrile, adds the anhydrous acetonitrile of 1-FMOC-3-pyrroles's methyl alcohol then, wherein: the mol ratio of vitamin H glycol phosphoramidite and 1-FMOC-3-pyrroles's methyl alcohol is 10: 1～1: 10; Add an amount of 5-ethylmercapto group tetrazole (ETT) activator, the concentration that makes the activator anhydrous acetonitrile of formation is 0.25mol/L; After at room temperature stirring 30 minutes, carry out reduced pressure distillation to Powdered; Add excessive oxidation solution, described oxidizing solution is to be dissolved in tetrahydrofuran (THF) by iodine: water: form in the mixed solvent of triethylamine=8: 1: 1, the mass percent concentration of solution is 4%; Stir under the room temperature after 10 minutes, be reduced to neutrality with Sulfothiorine, decompression steams tetrahydrofuran (THF) then, with chloroform extraction, organic layer behind anhydrous sodium sulfate drying, the evaporated under reduced pressure organic solvent; Add 10% ammonium hydroxide deprotection at last, decompression is neutralized to neutrality with 0.2M hydrochloric acid after steaming excessive ammonia, puts into-4 ℃ refrigerator is standby to get final product, and the chemical equation of present embodiment is as follows:
Similarly synthetic method also is applicable to have saturated or unsaturated hydro carbons substituting group on 4; aromatic series (comprising heterocycle) class substituting group; hydro carbons and/or aromatic series (comprising heterocycle) ether or thioether class substituting group; the halogen substituting group; one-level or secondary alcohols substituting group through protected silane; amine substituting group through FMOC or BOC protection; the tertiary amine of symmetrical or asymmetric replacement; acid amides or sulfonamides substituting group; the substituent 1-FMOC-3-pyrroles's methyl alcohol of carboxylic acid esters saturated or unsaturated hydrocarbons replaces; the 1-FMOC-3-epolamine; 1-FMOC-3-pyrroles's propyl alcohol; 1-BOC-3-pyrroles's methyl alcohol; 1-BOC-3-epolamine or 1-BOC-3-pyrroles's propyl alcohol.
Embodiment 2: preparation has the unitary thiophene derivant monomer of biological activity
Vitamin H glycol phosphoramidite is dissolved in the anhydrous acetonitrile, adds the anhydrous acetonitrile of 3-thiophen(e)alcohol then, wherein: the mol ratio of vitamin H glycol phosphoramidite and 3-thiophen(e)alcohol is 10: 1～1: 10; Add an amount of 5-ethylmercapto group tetrazole (ETT) activator, the concentration that makes the activator anhydrous acetonitrile of formation is 0.25mol/L; After at room temperature stirring 30 minutes, carry out reduced pressure distillation to Powdered; Add excessive oxidation solution, described oxidizing solution is to be dissolved in tetrahydrofuran (THF) by iodine: water: form in the mixed solvent of triethylamine=8: 1: 1, the mass percent concentration of solution is 4%; Stir under the room temperature after 10 minutes, be reduced to neutrality with Sulfothiorine, decompression steams tetrahydrofuran (THF) then, with chloroform extraction, organic layer behind anhydrous sodium sulfate drying, the evaporated under reduced pressure organic solvent; Add 10% ammonium hydroxide deprotection at last, decompression is neutralized to neutrality with 0.2M hydrochloric acid after steaming excessive ammonia, puts into-4 ℃ refrigerator is standby to get final product, and the chemical equation of present embodiment is as follows:
Similarly synthetic method also is applicable to have saturated or unsaturated hydro carbons substituting group on 4; aromatic series (comprising heterocycle) class substituting group; hydro carbons and/or aromatic series (comprising heterocycle) ether or thioether class substituting group; the halogen substituting group; one-level or secondary alcohols substituting group through protected silane; amine substituting group through FMOC or BOC protection; the tertiary amine of symmetrical or asymmetric replacement; acid amides or sulfonamides substituting group; the substituent 3-thiophen(e)alcohol of carboxylic acid esters saturated or unsaturated hydrocarbons replaces; 3-thiophene ethanol or 3-thiophene propyl alcohol.
Embodiment 3: preparation is used for the electric polypyrrole of biosensor
Microelectrode array is based upon on the logic circuit chip (seeing shown in Figure 1) of independent addressing, each electrode on this circuit is connected by the transistorized switch of complementary metal oxide semiconductor (CMOS), by sending the electronic address signal to the common junction circuit and then to SRAM (SRAM) conducting this switch relevant with each electrode; Microelectrode array is placed in the fluid reactor, and the reaction solution in the reactor is 1～10 times the pyrroles who has the substituent pyrrole monomer of vitamin H and its molar weight who prepares and mixes as electrolytical Repone K in right amount; Open with 10 milliseconds, the pulse of 10 milliseconds of passes, at 1.5 volts, the logical circuit control of 30 second total time down, the electric polypyrrole of biologically active generates rapidly in the electrode surface original position immediately, dries up with nitrogen after washed with de-ionized water and promptly gets the functionalization microelectrode array that is used for biosensor, and Fig. 2 is the photo of chamber light lower electrode, and (electrode of 16 micron diameters amplifies through 100 times of optics and obtains, wherein circle is an electrode, is isolator around the electrode).
Embodiment 4: preparation is used for the conductive polythiophene of biosensor
Microelectrode array is based upon on the logic circuit chip (seeing shown in Figure 1) of independent addressing, each electrode on this circuit is connected by the transistorized switch of complementary metal oxide semiconductor (CMOS), by sending the electronic address signal to the common junction circuit and then to SRAM (SRAM) conducting this switch relevant with each electrode; Microelectrode array is placed in the fluid reactor, and the reaction solution in the reactor is 1～10 times the thiophene that has the substituent thiophene monomer of vitamin H and its molar weight for preparing and mixes as electrolytical Repone K in right amount; Open with 10 milliseconds, the pulse of 10 milliseconds of passes, at 2.0 volts, the logical circuit control of 30 second total time down, the conductive polythiophene of biologically active generates rapidly in the electrode surface original position immediately, dries up with nitrogen after washed with de-ionized water and promptly gets the functionalization microelectrode array that is used for biosensor, and Fig. 3 is the photo of chamber light lower electrode, and (electrode of 16 micron diameters amplifies through 50 times of optics and obtains, wherein circle is an electrode, is isolator around the electrode).
The electrode that uses among the embodiment is platinum, can be substituted by iridium or other metals (for example: palladium, gold and silver, copper, mercury, nickel, zinc, titanium, tungsten, aluminium) or stainless steel, metal alloy, carbon nanotube, vitreous carbon, reticulated vitreous carbon, graphite, doping oxide, indium tin oxide, silicon oxide, gallium arsenide semiconductor, metal-doped polymkeric substance or stupalith.
Claims (10)
1. a conductive polymers that is used for biosensor is characterized in that, has following general formula:
R=S in the general formula or NH;
R1 in the general formula is
N wherein is 1,2 or 3; X represents the biological activity unit;
R2 in the general formula is a hydrogen, or saturated or unsaturated hydro carbons substituting group, fragrant same clan substituting group, hydro carbons and/or aromatic ether or thioether class substituting group, halogen substituting group, through the one-level of protected silane or secondary alcohols substituting group, through the carboxylic acid esters substituting group of tertiary amine, acid amides or the sulfonamides substituting group of amine substituting group, symmetry or the asymmetric replacement of FMOC or BOC protection, saturated or unsaturated hydrocarbons replacement.
2. the conductive polymers that is used for biosensor according to claim 1 is characterized in that, described biological activity unit is DNA, RNA, peptide, protein, enzyme, coenzyme, antibody, tissue or cell.
3. the conductive polymers that is used for biosensor according to claim 2 is characterized in that, described biological activity unit is vitamin H-vitamin H.
4. the described preparation method who is used for the conductive polymers of biosensor of claim 1 is characterized in that, comprises following concrete steps:
A) preparation has the unitary conductive polymers monomer of biological activity
To have the unitary glycol phosphoramidite of biological activity is dissolved in the anhydrous acetonitrile, add the monomeric anhydrous acetonitrile of conductive polymers then, wherein: having unitary glycol phosphoramidite of biological activity and the monomeric mol ratio of conductive polymers is 10: 1～1: 10; Add an amount of 5-ethylmercapto group tetrazole (ETT) activator, the concentration that makes the activator anhydrous acetonitrile of formation is 0.25mol/L; After at room temperature stirring 20～40 minutes, carry out reduced pressure distillation to Powdered; Add excessive oxidation solution, described oxidizing solution is to be dissolved in tetrahydrofuran (THF) by iodine: water: form in the mixed solvent of triethylamine=8: 1: 1, the mass percent concentration of solution is 4%; After stirring 5～15 minutes under the room temperature, be reduced to neutrality with Sulfothiorine, then the decompression steam tetrahydrofuran (THF), with chloroform extraction, organic layer behind anhydrous sodium sulfate drying, the evaporated under reduced pressure organic solvent; Add 10% ammonium hydroxide deprotection at last, be neutralized to neutrality with 0.2M hydrochloric acid after decompression steams excessive ammonia, it is standby to put into-4 ℃ of refrigerators;
B) microelectrode array is based upon on the logic circuit chip of independent addressing, each electrode on this circuit is connected by the transistorized switch of complementary metal oxide semiconductor (CMOS), by sending the electronic address signal to the common junction circuit and then to SRAM (SRAM) conducting this switch relevant with each electrode; Microelectrode array is placed in the fluid reactor, and the reaction solution in the reactor is 1～10 times the not modified conductive polymers monomer that has the unitary conductive polymers monomer of biological activity and its molar weight for preparing of step a) and mixes as electrolytical Repone K in right amount; Under logical circuit control, open with 10 milliseconds, the pulse of 10 milliseconds of passes, at 0.5～1.5 volt, in 30 second total time, the conductive polymers of biologically active promptly is engraved in the electrode surface original position and generates, and dries up with nitrogen after washed with de-ionized water and promptly gets the functionalization microelectrode array that is used for biosensor.
5. the preparation method who is used for the conductive polymers of biosensor according to claim 4, it is characterized in that the described unitary glycol phosphoramidite of biological activity that has is meant and has the unitary glycol phosphoramidite of DNA, RNA, peptide, protein, enzyme, coenzyme, antibody, tissue or cell.
6. the preparation method who is used for the conductive polymers of biosensor according to claim 5 is characterized in that, the described unitary glycol phosphoramidite of biological activity that has is meant vitamin H glycol phosphoramidite.
7. the preparation method who is used for the conductive polymers of biosensor according to claim 4 is characterized in that, described conductive polymers monomer is that general formula is:
Pyrrole derivative, n wherein is 1,2 or 3; R2 wherein is hydrogen or saturated or unsaturated hydro carbons substituting group, fragrant same clan substituting group, hydro carbons and/or aromatic ether or thioether class substituting group, halogen substituting group, through the one-level of protected silane or secondary alcohols substituting group, through the carboxylic acid esters substituting group of tertiary amine, acid amides or the sulfonamides substituting group of amine substituting group, symmetry or the asymmetric replacement of FMOC or BOC protection, saturated or unsaturated hydrocarbons replacement.
8. the preparation method who is used for the conductive polymers of biosensor according to claim 4 is characterized in that, described conductive polymers monomer is that general formula is:
Thiophene derivant, n wherein is 1,2 or 3; R2 wherein is hydrogen or saturated or unsaturated hydro carbons substituting group, fragrant same clan substituting group, hydro carbons and/or aromatic ether or thioether class substituting group, halogen substituting group, through the one-level of protected silane or secondary alcohols substituting group, through the carboxylic acid esters substituting group of tertiary amine, acid amides or the sulfonamides substituting group of amine substituting group, symmetry or the asymmetric replacement of FMOC or BOC protection, saturated or unsaturated hydrocarbons replacement.
9. the preparation method who is used for the conductive polymers of biosensor according to claim 4, it is characterized in that described electrode is metal, stainless steel, metal alloy, carbon nanotube, vitreous carbon, reticulated vitreous carbon, graphite, doping oxide, indium tin oxide, silicon oxide, gallium arsenide semiconductor, metal-doped polymkeric substance or stupalith.
10. the preparation method who is used for the conductive polymers of biosensor according to claim 9 is characterized in that described electrode is a platinum.
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