CN101824225B - Tussah silk peptide-glutin blend film and preparation method - Google Patents
Tussah silk peptide-glutin blend film and preparation method Download PDFInfo
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- CN101824225B CN101824225B CN2010101199122A CN201010119912A CN101824225B CN 101824225 B CN101824225 B CN 101824225B CN 2010101199122 A CN2010101199122 A CN 2010101199122A CN 201010119912 A CN201010119912 A CN 201010119912A CN 101824225 B CN101824225 B CN 101824225B
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- film
- tussah silk
- peptide
- blend film
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- 239000000203 mixture Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 10
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000007062 hydrolysis Effects 0.000 claims abstract description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 5
- 108010022355 Fibroins Proteins 0.000 claims description 14
- 238000000502 dialysis Methods 0.000 claims description 12
- 239000004033 plastic Substances 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 108010010803 Gelatin Proteins 0.000 claims description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 4
- 238000013016 damping Methods 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000008399 tap water Substances 0.000 claims description 4
- 235000020679 tap water Nutrition 0.000 claims description 4
- 239000004902 Softening Agent Substances 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 2
- 206010033546 Pallor Diseases 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003431 cross linking reagent Substances 0.000 abstract 1
- SYUXAJSOZXEFPP-UHFFFAOYSA-N glutin Natural products COc1c(O)cc2OC(=CC(=O)c2c1O)c3ccccc3OC4OC(CO)C(O)C(O)C4O SYUXAJSOZXEFPP-UHFFFAOYSA-N 0.000 abstract 1
- 239000004014 plasticizer Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 235000013305 food Nutrition 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Abstract
The invention relates to a tussah silk peptide-glutin blend film and a preparation method. The main technology comprises the following steps: processing tussah silk protein by a calcium nitrate salt method; preparing tussah silk peptide through hydrolysis, blanching and centrifugation; preparing the tussah silk peptide with glutin, plasticizers and cross linking agents to obtain film forming liquid; taking reaction for a certain time at a proper temperature; pouring and placing the film forming liquid into a container with edge frames to be dried; and peeling the film to obtain the blend film. The invention uses tussah waste cocoon shells as raw materials, provides a novel path for the utilization of changing waste materials into useful materials, and has the advantages of scientific and reasonable technology, simplicity, easy implementation and low cost. The film has the advantages of good extensibility, good mechanical performance and semitransparency. The products are safe and edible.
Description
Technical field
Invention belongs to food processing technology field for the preparation method of peptide of tussur silk-glutin blend film.
Background technology
Peptide of tussur silk contains 18 seed amino acids that human body had nutritive value, and wherein 8 seed amino acid content of needed by human are abundant.The molecular structure of peptide of tussur silk is a spiral, has special ultrastructure and multilevel hierarchy, and these structures make it possess good oxygen flow, moisture retention, and has good film forming properties.
Edible film is prone to not had any environmental pollution by biological degradation; Edible film can be used as the carrier of flavour of food products material, sweet taste material, nutrition-fortifying agent, inhibitor, anti-microbial agents; Can carry out individual packaging to food easily, like packing polly seed nucleole, nut meat, fruits and vegetables etc.; Be applied to plastics packed internal layer, the hazard residue thing is to the migration of food in the minimizing plastics; Have good barrier property, reasonably the film material form can effectively regulate and control food between the shelf lives with external environment and the transmission of matter between inner the composition, to improve food quality, prolong shelf-life, and save wrapping material.
Summary of the invention
The object of the invention just provides and a kind of waste is utilized again; Through simple machining process; Have good extensibility and transparency; If the edible nutritive substance that can be used as is not if discarded have any pollution and can all be degraded the preparation method of widely used peptide of tussur silk-glutin blend film by mikrobe environment.
The present invention is for realizing above-mentioned purpose, and the technical scheme that is adopted is: a kind of edible blend film, and it contains following component:
Preparing method according to a kind of blend film of the present invention is:
(1) peptide of tussur silk, gelatin and softening agent preparation film forming liquid behind magnetic agitation 5min-1h, are incubated 5min-1h in 40-80 ℃ of water-bath;
(2) liquid temp to be filmed drops to 40-70 ℃, regulates pH5.0-9.0, adds linking agent, the insulation 5min-2h that stirs,
(3) under the room temperature condition film forming liquid is filtered drying and forming-film.
Preparing method's one optimal technical scheme according to a kind of blend film of the present invention: the preparation method of said peptide of tussur silk is: getting concentration is the Powdered tussah silk fibroin of 10mg/mL; Add a certain amount of damping fluid; Regulate pH to 9, add the Sumizyme MP of 8% (w/w) again, in 50 ℃ of water-baths behind the hydrolysis 4h; 85 ℃ of the enzyme 15min that go out, the centrifugal 10min of 6000rpm, get supernatant, promptly get peptide of tussur silk through lyophilize.
Preparing method's one optimal technical scheme according to a kind of blend film of the present invention: the preparation method of said Powdered tussah silk fibroin is: the sodium carbonate solution of the employing of the cocoon shell after the removal of impurities 0.5% is carried out secondary degumming; Fibrous fibroin 1-10g after will coming unstuck; Divide and place the constantly nitrocalcite of the molten state of heating of 20-300g for 10 times; To all dissolvings; It is the 7000-14000 dialysis tubing that molecular weight is blocked in employing, and with tap water, deionized water dialysis, dialysis promptly gets Powdered tussah silk fibroin after finishing in succession.
Preparing method's one optimal technical scheme according to a kind of blend film of the present invention: the method for said drying at room temperature Cheng Mo is: draw 2mL film forming liquid on plastic carrier; Be coated with into the uniform film of distribution; Remove 20min-4h in the air dry oven that bubble is placed on 20-60 ℃, take off film after to be dried.
Beneficial effect of the present invention is:
1, raw material of the present invention is a tussah piques shell, for improving its value, rationally utilizes its nutritive value that an effective way is provided;
2, technology of the present invention is reasonable, simple possible, and cost is low;
3, the peptide of tussur silk edible film has good ductility, mechanicalness, and transparency is better;
4, this product safety is reliable, and not only having can be used as wrapping material, to use but also can be used as nutritive substance edible, has a extensive future.
Embodiment
Embodiment 1
The present invention combines the implementing process instance to further specify:
1, fibrous tussah silk fibroin 5g; Divide to place the constantly Ca (NO3) 2 of the molten state of heating of 130g for 10 times,, adopt the MWCO14000 dialysis tubing to all dissolvings; With tap water, deionized water dialysis, dialysis promptly gets Powdered tussah silk fibroin after finishing in succession.
2, get the Powdered tussah silk fibroin 100mL that concentration is 10mg/mL; Add a certain amount of damping fluid; Regulate pH to 9, add the Sumizyme MP of 8% (w/w) again, in 50 ℃ of water-baths behind the hydrolysis 4h; 85 ℃ of the enzyme 15min that go out, the centrifugal 10min of 6000rpm, get supernatant, promptly get peptide of tussur silk through lyophilize.
3,, add 2% Sorbitol Powder and glycerine (1: 1), magnetic agitation 5min with 2% tussah silk peptide peptide solution 10mL and 2% gelatin solution 10mL uniform mixing; 60 ℃ of water bath heat preservation 20min treat that temperature drops to 50 ℃, behind the adjusting pH to 7.0; The linking agent glutamine transaminage of adding 2%; Stir, 50 ℃ of insulation 1h, room temperature is filtered the film forming liquid that promptly gets suitable concentration.
4, draw 2mL film forming liquid respectively on plastic carrier, be coated with into the film that 10cm * 10cm is evenly distributed, remove 3h in the air dry oven that bubble is placed on 40 ± 2 ℃, take off film after to be dried.
Embodiment 2
1, fibrous tussah silk fibroin 3g divides the 90%Ca (NO3) that places continuous heating for 10 times 2 solution, treats basic dissolving, adopts the MWCO7000 dialysis tubing, and with tap water, deionized water dialysis, dialysis promptly gets Powdered tussah silk fibroin after finishing in succession.
2, get the Powdered tussah silk fibroin 100mL that concentration is 10mg/mL; Add a certain amount of damping fluid; Regulate pH to 9.5, add the Sumizyme MP of 7.5% (w/w) again, in 50 ℃ of water-baths behind the hydrolysis 4h; 85 ℃ of the enzyme 15min that go out, the centrifugal 10min of 6000rpm, get supernatant, promptly get peptide of tussur silk through lyophilize.
3,, add 1% Sorbitol Powder and glycerine (2: 1), magnetic agitation 5min with 2.5% tussah silk peptide peptide solution 10mL and 1.5% gelatin solution 10mL uniform mixing; 70 ℃ of water bath heat preservation 10min treat that temperature drops to 50 ℃, behind the adjusting pH to 7.0; The linking agent glutamine transaminage of adding 3%; Stir, 50 ℃ of insulation 1h, room temperature is filtered the film forming liquid that promptly gets suitable concentration.
4, draw 2mL film forming liquid respectively on plastic carrier, be coated with into the film that 10cm * 10cm is evenly distributed, remove 2h in the air dry oven that bubble is placed on 45 ± 2 ℃, take off film after to be dried.
The above; Be merely the preferable embodiment of the present invention; But protection scope of the present invention is not limited thereto; Any technician who is familiar with the present technique field is equal to replacement or change according to technical scheme of the present invention and inventive concept thereof in the technical scope that the present invention discloses, all should be encompassed within protection scope of the present invention.
Claims (5)
1. a blend film is characterized in that, it contains following component:
2. a kind of blend film according to claim 1 is characterized in that: the method for preparing blend film is:
(1) peptide of tussur silk, gelatin and softening agent preparation film forming liquid behind magnetic agitation 5min-1h, are incubated 5min-1h in 40-80 ℃ of water-bath;
(2) liquid temp to be filmed drops to 40-70 ℃, regulates pH5.0-9.0, adds linking agent, the insulation 5min-2h that stirs,
(3) under the room temperature condition film forming liquid is filtered drying and forming-film.
3. a kind of blend film according to claim 2 is characterized in that: the preparation method of said peptide of tussur silk is:
Getting concentration is the Powdered tussah silk fibroin of 10mg/mL, adds a certain amount of damping fluid, regulates pH to 9; The Sumizyme MP that adds 8% (w/w) again; In 50 ℃ of water-baths behind the hydrolysis 4h, 85 ℃ of the enzyme 15min that go out, the centrifugal 10min of 6000rpm, get supernatant, promptly get peptide of tussur silk through lyophilize.
4. a kind of blend film according to claim 3 is characterized in that: the preparation method of said Powdered tussah silk fibroin is:
The sodium carbonate solution of the employing of the cocoon shell after the removal of impurities 0.5% is carried out secondary degumming; Fibrous fibroin 1-10g after will coming unstuck; Divide to place the constantly nitrocalcite of the molten state of heating of 20-300g for 10 times, to all dissolvings, adopting and blocking molecular weight is the 7000-14000 dialysis tubing; With tap water, deionized water dialysis, dialysis promptly gets Powdered tussah silk fibroin after finishing in succession.
5. a kind of blend film according to claim 2; It is characterized in that: the film forming method of said drying at room temperature is: draw 2mL film forming liquid on plastic carrier; Be coated with into the uniform film of distribution, remove 20min-4h in the air dry oven that bubble is placed on 20-60 ℃, take off film after to be dried.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101199122A CN101824225B (en) | 2010-03-03 | 2010-03-03 | Tussah silk peptide-glutin blend film and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101199122A CN101824225B (en) | 2010-03-03 | 2010-03-03 | Tussah silk peptide-glutin blend film and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101824225A CN101824225A (en) | 2010-09-08 |
| CN101824225B true CN101824225B (en) | 2012-06-20 |
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| CN2010101199122A Expired - Fee Related CN101824225B (en) | 2010-03-03 | 2010-03-03 | Tussah silk peptide-glutin blend film and preparation method |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102133425B (en) * | 2011-03-13 | 2014-05-14 | 苏州大学 | Tussah silk fibroin film and preparation method thereof |
| CN103506162B (en) * | 2013-10-09 | 2015-03-25 | 华东理工大学 | Preparation method for silver/silver halide films |
| CN107307062B (en) * | 2017-08-08 | 2020-06-16 | 福建农林大学 | Konjac glucomannan shrimp preservative and preparation method thereof |
| CN107812239A (en) * | 2017-09-28 | 2018-03-20 | 广东医科大学 | A kind of preparation method of tussah silk peptide collagen compound rest |
| CN108578791A (en) * | 2018-04-24 | 2018-09-28 | 吴刚 | A kind of hirudin is modified the preparation method of anticoagulant material |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121836A (en) * | 1995-07-25 | 1996-05-08 | 苏州丝绸工学院 | Wound surface protection film and its preparation method |
| US6110590A (en) * | 1998-04-15 | 2000-08-29 | The University Of Akron | Synthetically spun silk nanofibers and a process for making the same |
| CN1467248A (en) * | 2002-06-19 | 2004-01-14 | 独立行政法人农业生物资源研究所 | Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers |
| CN101199314A (en) * | 2006-12-12 | 2008-06-18 | 河南农业大学 | Novel process for preparing esculent film using glutamine transaminage modified protein |
-
2010
- 2010-03-03 CN CN2010101199122A patent/CN101824225B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121836A (en) * | 1995-07-25 | 1996-05-08 | 苏州丝绸工学院 | Wound surface protection film and its preparation method |
| US6110590A (en) * | 1998-04-15 | 2000-08-29 | The University Of Akron | Synthetically spun silk nanofibers and a process for making the same |
| CN1467248A (en) * | 2002-06-19 | 2004-01-14 | 独立行政法人农业生物资源研究所 | Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers |
| CN101199314A (en) * | 2006-12-12 | 2008-06-18 | 河南农业大学 | Novel process for preparing esculent film using glutamine transaminage modified protein |
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| CN101824225A (en) | 2010-09-08 |
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