CN101856223A - Minimally invasive electrode for in-vivo measurement of nerve action potential - Google Patents
Minimally invasive electrode for in-vivo measurement of nerve action potential Download PDFInfo
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- CN101856223A CN101856223A CN 201010203157 CN201010203157A CN101856223A CN 101856223 A CN101856223 A CN 101856223A CN 201010203157 CN201010203157 CN 201010203157 CN 201010203157 A CN201010203157 A CN 201010203157A CN 101856223 A CN101856223 A CN 101856223A
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- electrode
- action potential
- wicresoft
- minimally invasive
- nerve action
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Abstract
The invention discloses a minimally invasive electrode for in-vivo measurement of nerve action potential, and relates to a biologic (neurophysiologic) experimental device which is particularly suitable for directly measuring the action potential and conduction speed of in-vivo nerve of an animal. The minimally invasive electrode is characterized in that a sharp needle-like metal electrode pair is adopted; a handhold part is made of an insulating material; and a leading electrode pair connected with the needle-like electrode pair is further provided. The minimally invasive electrode overcomes the defect of non-available in-vivo measurement or serious in-vivo trauma; and meanwhile, the electrode can repeatedly measure the action potential conduction speed of the same nerve in different time intervals, has the characteristics of in-vivo measurement, minimal invasion, repetitiveness and convenient use and solves the problems of in-vivo measurement of the action potential of sciatic nerves and nervelet of animals, such as rats, mice, guinea pigs, dogs, monkeys and the like. The invention also relates to a process for manufacturing the minimally invasive electrode.
Description
Technical field
The present invention relates to be used to be determined at Wicresoft's electrode of somatic nerves action potential, be particularly suitable for directly measuring action potential and the conduction velocity of animal at somatic nerves.The invention still further relates to the technology of manufacturing according to Wicresoft of the present invention electrode.
Background technology
Measuring nerve action potential and conduction velocity thereof is biomedical, pharmacology, particularly the common technology in the neuro physiology research.The common technology of at present existing multiple direct mensuration nerve action potential: the assay method of (1) stripped nerve trunk action potential, but this method need will be measured behind the neural separation cut, can only measure some characteristics after nerve breaks away from animal, can't measure neural at bulk properties; (2) at the traumatic nerve trunk action potential of body assay method, this method can cause the major injury or the death of laboratory animal, and this method causes nerve trunk to be exposed in the air, make nerve trunk dehydration or downright bad, influence result's accuracy, can not satisfy the needs of measuring nerve action potential at body repeatedly.In order to observe the situation of change of sciatic conduction velocity, need successively repeatedly to measure the conduction velocity of action potential, and present assay method and electrode can't be satisfied with above needs in the experiment along with the experiment process.
Summary of the invention
Consider above requirement, the purpose of this invention is to provide a kind of Wicresoft's electrode, have in body measurement, Wicresoft, characteristics easy to use in the body measurement nerve action potential.
In order to realize above purpose, this electrode is made up of totally three parts the leading electrode of, intermediary handgrip part, rear end foremost needle electrode.
Wherein, needle electrode is to being two parallel elongated needles foremost, and sharp tip can be slope shape or taper shape, and needle body can be made up of various conductors such as ferrum, silver, copper, gold, platinum, Nickel, can be hollow or solid.Its preferred length is 5～200mm, more preferably between 10～100mm.Its preferred diameter is 0.01～3mm, more preferably at 0.1～1mm.
The middle part is a handgrip part, to right with leading electrode, is used for hand-held or fixing between needle electrode.Can form by acceptable megohmite insulants such as plastics, rubber, PVC.Be shaped as rectangular-shaped or the acceptable different shape, as shown in Figure 1.
Rearmost end is that leading electrode is right, insulator is exposed at rear portion at handgrip part, and inside links to each other with the crosspointer of front end, is used to guide the signal of telecommunication of crosspointer, can form by various conductors such as ferrum, silver, copper, gold, platinum, Nickel, be shaped as cylindric or the acceptable different shape.
Experiment has proved the right length of needle electrode between 5～200mm, and the diameter of pin is suitable between 0.01～3mm, can solve animal sciatic nerve such as rat, mice, Cavia porcellus, dog, monkey and nervelet action potential in the body measurement problem.
Description of drawings
Fig. 1 is a structure chart of the present invention
Fig. 2 for the C of Fig. 1 of the present invention to view (side sight)
Among Fig. 1 and Fig. 2: 1, needle electrode is right; 2, handgrip part; 3, leading electrode is right.
The specific embodiment
The mensuration of embodiment one rat sciatic nerve action potential conduction velocity
Each treated animal pentobarbital sodium intraperitoneal anesthesia during mensuration, 35mg/kg is prostrate fixing.With the present invention is stimulating electrode, behind 75% alcohol disinfecting, gets two stimulating electrodes and inserts near sciatic nerve nest place sciatic nerve-trunk, the nerve trunk calf section start record distance D (mm) respectively.With the present invention is leading electrode, and the gastrocnemius belly of muscle is inserted near near diseased of the sciatic nerve in the sterilization back, the operation of recording current potential.Calculate T1 incubation period (neural nest), T2 that two stimulation points produce muscle action potential respectively, the ms of unit.The BL-420E that equipment adopts Chengdu Tai Meng bio tech ltd to produce
+Type biological function experimental system, stimulus signal is selected 1-100mv, and 0.015ms), single stimulation mode, scanning speed are 0.01 second (s), 10kHz filtering, pilot signal is amplified 50-100 doubly, and the conduction velocity computing formula is:
V(m/s)＝D(mm)/(T1-T2)(ms)。
The mensuration of embodiment two rat sensory nerve conduction velocitys (SNCV)
Each treated animal pentobarbital sodium intraperitoneal anesthesia during mensuration, 35mg/kg is prostrate fixing.Conventional method is measured sensory nerve conduction velocity, is stimulating electrode with the present invention, 75% alcohol disinfecting, and respectively at gastrocnemius near-end and quadriceps femoris proximal stimulus, stimulus signal is selected 1.5mv, 0.15ms, twice stimulation point distance is D (about 25mm).With the present invention is leading electrode, inserts gastrocnemius belly of muscle 0.5mm behind 75% alcohol disinfecting, the BL-420E that equipment adopts Chengdu Tai Meng bio tech ltd to produce
+Type biological function experimental system, pilot signal is amplified 200 times, scanning speed 0.01s, 10kHz filtering.Write down the corresponding F ripple of two stimulation points T1 incubation period, T2, unit is ms, and computing formula is:
SNCV(m/s)＝D/(T1-T2)。
Claims (8)
1. Wicresoft's electrode in the body measurement nerve action potential is used for measuring, Wicresoft's electrode of the big nerve trunk action potential of animal particularly, it is characterized in that its needle electrode with sharp tip to, leading electrode to and handgrip part.
2. the Wicresoft's electrode in the body measurement nerve action potential as claimed in claim 1 is characterized in that its front end has two spicules of parallel distribution.
3. the Wicresoft's electrode in the body measurement nerve action potential as claimed in claim 2 is characterized in that described spicule is made of metal.
4. as claim 1,2 or 3 described Wicresoft's electrodes, it is characterized in that described spicule has sharp tip in the body measurement nerve action potential.
5. as any one described Wicresoft's electrode in the body measurement nerve action potential in the claim 2 to 4, it is characterized in that the right length of described needle electrode between 5～200mm, the diameter of main body is between 10mm～100mm.
6. as any one described Wicresoft's electrode in the body measurement nerve action potential in the claim 2 to 5, the diameter that it is characterized in that described spicule is between 0.01～3mm, and preferred diameter is about 0.1～1mm.
7. as any one described Wicresoft's electrode in the body measurement nerve action potential of above claim 1 to 5, the handgrip part that it is characterized in that described Wicresoft's electrode in the body measurement nerve action potential can be circular, semicircle, square, rectangle or triangle.
As above patent require 1 to 6 any one described Wicresoft's electrode in the body measurement nerve action potential, it is characterized in that the leading electrode of described Wicresoft's electrode in the body measurement nerve action potential can be circular, semicircle, square, rectangle, triangle, T type or hook-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010203157 CN101856223A (en) | 2010-06-18 | 2010-06-18 | Minimally invasive electrode for in-vivo measurement of nerve action potential |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010203157 CN101856223A (en) | 2010-06-18 | 2010-06-18 | Minimally invasive electrode for in-vivo measurement of nerve action potential |
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| CN101856223A true CN101856223A (en) | 2010-10-13 |
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| CN 201010203157 Pending CN101856223A (en) | 2010-06-18 | 2010-06-18 | Minimally invasive electrode for in-vivo measurement of nerve action potential |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5161533A (en) * | 1991-09-19 | 1992-11-10 | Xomed-Treace Inc. | Break-apart needle electrode system for monitoring facial EMG |
| US6139545A (en) * | 1998-09-09 | 2000-10-31 | Vidaderm | Systems and methods for ablating discrete motor nerve regions |
| JP2006334106A (en) * | 2005-06-01 | 2006-12-14 | Nippon Medical School | Bipolar stimulating electrode |
| CN201019744Y (en) * | 2007-01-05 | 2008-02-13 | 张炳谦 | Special-purpose monopole double-needle electrode for electromyogram |
-
2010
- 2010-06-18 CN CN 201010203157 patent/CN101856223A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5161533A (en) * | 1991-09-19 | 1992-11-10 | Xomed-Treace Inc. | Break-apart needle electrode system for monitoring facial EMG |
| US6139545A (en) * | 1998-09-09 | 2000-10-31 | Vidaderm | Systems and methods for ablating discrete motor nerve regions |
| JP2006334106A (en) * | 2005-06-01 | 2006-12-14 | Nippon Medical School | Bipolar stimulating electrode |
| CN201019744Y (en) * | 2007-01-05 | 2008-02-13 | 张炳谦 | Special-purpose monopole double-needle electrode for electromyogram |
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Open date: 20101013 |