CN101871889B - Liquid sample detection device utilizing LED light source - Google Patents

Liquid sample detection device utilizing LED light source Download PDF

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Publication number
CN101871889B
CN101871889B CN2010101972407A CN201010197240A CN101871889B CN 101871889 B CN101871889 B CN 101871889B CN 2010101972407 A CN2010101972407 A CN 2010101972407A CN 201010197240 A CN201010197240 A CN 201010197240A CN 101871889 B CN101871889 B CN 101871889B
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light source
led light
liquid sample
detection device
led
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CN101871889A (en
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张琪
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Abstract

The invention discloses a liquid sample detection device utilizing an LED light source, comprising the LED light source and an optical signal collector, wherein, the LED light source is used for providing light needed in entering a liquid sample; and the optical signal collector is used for collecting the light transmittance that the LED light source passes through the liquid sample; the LED light source and the optical signal collector are controlled by one data collection controller which controls the startup of the LED light source, and controls the optical signal collector to collect the light transmittance at a preset time after the LED light source is started. The liquid sample detection device utilizing the LED light source of the invention has high-precision detection result, low cost and short detection time, and does not need special processing on the LED light source; and the service life of the LED is greatly prolonged.

Description

Use the liquid sample detection device of led light source
Technical field
The present invention relates to a kind of liquid sample detection device, specifically relate to a kind of device that uses led light source to utilize transmittance that the liquid sample internal substance of little quality is detected.
Background technology
Adopting light to detect the means of differentiating material is technology commonly used in the microscopic fields; Usual way is in a container, to hold carrier fluid; And on optical detection device, detect the transmittance of this carrier fluid; And then the sample material of required detection is dissolved in this carrier fluid, on optical detection device, detect the transmittance of solution again, and the main attenuate light wave band in the testing result and the decay intensity of this band of light relatively.Judge the kind of the sample material that is detected by this comparative result.Therefore, for obtaining testing result accurately, stable light source is very important.Common detecting instrument light source adopts Halogen lamp LED or incandescent lamp more at present, because Halogen lamp LED or incandescent lamp all are thermal light source, measures so the preheating of before detecting instrument work, should starting shooting earlier made light source reach stable could the beginning in 30 minutes, causes and uses inconvenience.In addition, the serviceable life of Halogen lamp LED or incandescent lamp is short, and because the booting wait overlong time is left after operating personnel often start shooting to cause to detect and incured loss through delay, the loss of Halogen lamp LED or incandescent lamp is strengthened, and has further shortened serviceable life.
Chinese patent document CN201041544Y discloses a kind of analyser light-source structure; Be made up of circuit board, a 3-5 light emitting diode (LED), lamp cover, LED is connected with circuit board, and lamp overlaps corresponding LED and forms lamp hole; LED is installed in the lamp hole, and the optical filter on the light direct projection analyser rotating disk.The problem that this prior art exists is; Because the brightness of LED and temperature are inversely proportional to; So along with the brightness of the temperature rising LED of LED diminishes, in the place that measuring accuracy is had relatively high expectations, existing way is that LED lights the back and waits for several minutes and just detect up to the brightness of LED is relatively stable; Cause staff's stand-by period longer so on the one hand, use inconvenient; The life consumption of LED lamp is also bigger on the other hand; In addition, the brightness of LED is still in decay quickly in measuring process, and the data accuracy that the brightness of LED is measured when low more is poor more.That is to say that the precision as a result that existing led light source pick-up unit and detection method are measured is lower, error is bigger.
It is to establish the calibration cell ambient light source to play the stabilized light source action of temperature that a kind of solution is arranged at present, so install not only cost increase, and volume becomes big.
Chinese patent document CN85107724A discloses a kind of light measurer; Driving section with constant current source; By this driving section transmission LED, again the light that penetrates from LED through receiving light as the material that the function of mensurated amount changes with the optical transmission characteristic, be transformed into the light accepting part of electric weight then; Detect the voltage detecting means of the forward voltage of above-mentioned LED; Through the way that the output of above-mentioned light accepting part is carried out revisal by the output of above-mentioned voltage detecting means, the error at measurment that occurs when producing wavelength change in the temperature variation of above-mentioned LED to eliminate, and through signal processing part mensurated amount is performed calculations.The problem that this prior art exists is; Need a wavelength test section to detect the wavelength of LED and in signal processing part, need the error relation of storage in advance and forward voltage could the revisal measuring error; And accurately draw this error relation is difficulty very, and this light measurer structure relative complex, cost are high.
Japanese documentation JP2007198935 (A) discloses a kind of analytical equipment; Use LED to make light source; A reactive tank has the liquid maintaining part to keep fluid to be measured constant temperature, constant rotational speed; Light guide section is used for the light intensity that the homogenization led light source sends; Flash ranging bonding part is used for measuring light and passes the light intensity of liquid maintaining part to the exit position of light guide section, and signal processing part divisional processing is from the signal of flash ranging bonding part, and is true with regard to the prohibited data detection that the light intensity that has solved the led light source heterogeneityization causes like this.The problem that this prior art exists is; Need to use light guide section to come homogenization LED light intensity; And the variation of LED light intensity is not easy to carry out the homogenization processing more greatly; Therefore this analytical equipment still can't solve the low problem of led light source accuracy of detection effectively, and its structure also more complicated, cost are high.
Summary of the invention
For this reason; Technical matters to be solved by this invention be in the prior art in order to reach accurately that led light source is accurately measured and the problem that need carry out special processing to led light source, thereby a kind of liquid sample detection device that just can accurately detect the liquid sample inner material that need not carry out special processing to led light source is proposed.
For solving the problems of the technologies described above; The invention provides a kind of liquid sample detection device that uses led light source; Comprise the led light source that is used to provide the said liquid sample of incident institute light requirement, be used to gather the optical signal acquisition device that said led light source sees through the transmittance of said liquid sample, also comprise a data acquisition controller; Said data acquisition controller is connected with said led light source, controls the unlatching of said led light source; Said data acquisition controller also is connected with said optical signal acquisition device, and controls the schedule time or the time interval of said optical signal acquisition device after said led light source is opened and gather said transmittance data.
Said optical signal acquisition device is a photovoltaic converter.
Be connected with timing module with said data acquisition controller.
Said data acquisition controller is a single-chip microcomputer; Said single-chip microcomputer is controlled the unlatching of said led light source through electric signal; When said led light source is opened; The said timing module of said Single-chip Controlling picks up counting, and said single-chip microcomputer is controlled said optical signal acquisition device and gathered said transmittance data when said timing module timing reaches the said schedule time or time interval.
The said schedule time is 2 milliseconds to 2 seconds.
The said schedule time is 60% required time that 80% required time to said led light source brightness that said led light source brightness rises to peak value drops to peak value.
Said led light source is that the LED lamp of 260 nanometers and 380 nanometers is formed by sending wavelength.
The light that said LED lamp sends is through synthetic one road light of polariscope.
Technique scheme of the present invention is compared prior art and is had the following advantages,
First; The liquid sample detection device of use led light source of the present invention; It uses led light source to provide the said liquid sample of incident required light; Utilize the data acquisition controller that is provided with to control the unlatching of said led light source, control the schedule time or the time interval of said optical signal acquisition device after said led light source is opened simultaneously and gather said transmittance data.
The mensuration of the said schedule time or time interval is to consider in the prior art to use led light source to carry out liquid sample when detecting; Need wait until generally that the brightness of led light source is relatively stable and just detect; Then needed just can accomplish in several minutes during this period of time; Like this stand-by period oversize, and led light source is omnidistance in the process that liquid sample is detected opens, also consumed power in large quantities.Based on the purpose that addresses the above problem; The inventor is through discovering; In fact in metastable several minutes of said led light source entering brightness, also have one section less time interval, the brightness of led light source also is metastable in this time interval; So through using led light source that liquid sample is detected, it has the higher detection precision equally in this time interval.The interval promptly is the schedule time described in the application during this period of time.Through utilization to this time interval, just practiced thrift the tester and waited for that led light source gets into the several minutes clock times in the relatively stable interval of brightness, it has also made contribution detection time to practicing thrift on the whole from this aspect.
Mensuration just because of the above-mentioned schedule time or time interval; It need not carry out any special processing to led light source; Through confirming the relation along with change of time its light-source brightness variation (corresponding energy changing) of led light source with respect to detected sample; Confirm that led light source brightness is higher and be suitable for the detection that transmittance detects " stablize " time period (be the energy decay acutely and also energy of light source higher relatively); Guarantee in this time period detected sample is carried out the collection of transmittance data, thereby realize accurate detection liquid sample; Get into the decay section of energy of light source at led light source after; Said led light source is closed; In the time of need carrying out further sample detecting to the sample transmittance afterwards, open led light source again, promptly can get into the energy stabilization time period of led light source through the short time once more; When realization accurately detects the detected sample transmittance, shortened detection time equally on the whole like this.
In addition, use led light source of the present invention carries out the device that liquid sample detects, and need not add any equipment that led light source is carried out special processing, so it is lower to detect cost, device structure is simple.
Second; Use led light source of the present invention carries out the device that liquid sample detects; Use this device that liquid sample is detected, it has practiced thrift the time of detecting on the whole, thus the consumption of having practiced thrift electric energy to a great extent; Also make the life consumption of led light source reduce, thereby prolonged the serviceable life of led light source greatly.
The 3rd, use led light source of the present invention carries out the device that liquid sample detects, and LED brightness is higher, energy is higher and comparatively stable zone is detected owing to be chosen at, and detected transmittance data accuracy is high.
Description of drawings
For content of the present invention is more clearly understood, below according to a particular embodiment of the invention and combine accompanying drawing, the present invention is done further detailed explanation, wherein
Fig. 1 be led light source photosignal and conduction time graph of a relation;
The block scheme that Fig. 2 is to use the liquid sample detection device of led light source to constitute;
Fig. 3 is structure connection side's block diagram that timing module is set in the pick-up unit according to the invention.
Embodiment
Referring to Fig. 1; Shown is, and the relation curve of back time and brightness is opened in the LED energising, and wherein brightness is converted into voltage through opto-electronic conversion and representes intensity, and brightness moment reached peak value after as can be seen from Figure 1 the led light source energising was opened; One section brightness zone is relatively stably arranged in the interval range of the left and right sides of this peak value; Relatively stably behind the zone, the brightness of said led light source decays rapidly through this section brightness, in its brightness lasting about 2 seconds of whole process before the decay rapidly.This figure has just provided the synoptic diagram of the relation curve of led light source energising unlatching back time and brightness; Because 2 seconds time gap is longer for 2 milliseconds; So on 2 seconds time interval horizontal ordinate, dotted line is set; Thereby explain that this section is provided with the abridged time interval, but can not influence the peak shape variation of relation curve owing to this abridged time interval.
Referring to Fig. 2, the liquid sample detection device of the use led light source of present embodiment comprises by electrically driven (operated) led light source; Said led light source is used to provide the said liquid sample of incident required light, and a photovoltaic converter is used to gather the transmittance that said led light source sees through said liquid sample, and said led light source and said photovoltaic converter are by a Single-chip Controlling; The unlatching of the said led light source of said Single-chip Controlling, and control said photovoltaic converter is gathered said liquid sample in the said led light source unlatching back schedule time transmittance.
The principle of work of the liquid sample detection device of the use led light source of present embodiment is; The light of said led light source is injected in the said photovoltaic converter after passing said liquid sample again; Said liquid sample is carried out transmittance to be measured; Said photovoltaic converter is according to the difference of detected main attenuate light wave band, and the difference of optical attenuation intensity, detects contained material in the sample.
The method that the liquid sample detection device of the use led light source of present embodiment carries out the liquid sample detection is; Pick-up unit start preheating gets into duty; Said single-chip microcomputer sends power on signal and gives said led light source energising to open said led light source; The light that said led light source sends incides said liquid sample; The timing module (referring to Fig. 3) that said and said single-chip microcomputer is connected picks up counting when said led light source is opened, and said single-chip microcomputer is given the signal of an image data of said photovoltaic converter when said timing module timing reaches 80 milliseconds, and said photovoltaic converter is gathered the transmittance of said liquid sample; Whenever said led light source is closed in intact transmittance data acquisition under said Single-chip Controlling; Detect next time open said led light source again and according to above said method detect.Certain said single-chip microcomputer also can be given the signal of an image data of said photovoltaic converter when said timing module timing reaches 2 milliseconds or 2 seconds.
Because the specification of the LED lamp that different manufacturers is produced is different, continues roughly the same brightness variation characteristic in time though all have, the time that the LED lamp reaches peak value is difference to some extent.Therefore; Can confirm the moment of the transmittance of the said liquid sample of collection through the magnitude of LED brightness; Method is that the brightness that at first detects after the LED lamp is opened through said photovoltaic converter changes, and said photovoltaic converter is converted into voltage volt value with the luminance brightness of LED lamp and representes that the high more expression brightness of volt value is big more; Thereby can draw the time and the brightness relationship curve of the LED lamp of arbitrary specification, promptly can confirm the different brightness amplitude time corresponding in led light source unlatching back.
The liquid sample detection device of the use led light source of another one embodiment; The unlatching of the said led light source of said Single-chip Controlling, and control said photovoltaic converter a plurality of transmittance data that predetermined time interval is gathered said liquid sample in said led light source is opened the back schedule time.The said schedule time is 60% required time that 80% required time to said led light source brightness that said led light source brightness rises to peak value drops to peak value.Said predetermined time interval can be uniformly, also can be heterogeneous.
In another embodiment; Use the liquid sample detection device of led light source also to comprise a polariscope; Said led light source is that the LED lamp of 260 nanometers and 380 nanometers is formed by sending wavelength, and the light that said LED lamp sends incides said liquid sample through synthetic one road light of said polariscope.The liquid sample detection device of the use led light source of this embodiment can be used to detect DNA, RNA.
The liquid sample detection device testing result precision of use led light source of the present invention is high; And need not carry out special processing to led light source, cost is low; It is short to detect the time spent, does not need the long stand-by period, and because the LED service wear is little, prolong the serviceable life of LED greatly.
Obviously, the foregoing description only be for explanation clearly done for example, and be not qualification to embodiment.For the those of ordinary skill in affiliated field, on the basis of above-mentioned explanation, can also make other multi-form variation or change.Here need not also can't give exhaustive to all embodiments.And conspicuous variation of being extended out thus or change still are among the protection domain of the invention.

Claims (7)

1. liquid sample detection device that uses led light source; Comprise the led light source that is used to provide the said liquid sample of incident institute light requirement; Be used to gather the optical signal acquisition device that said led light source sees through the transmittance of said liquid sample; It is characterized in that: also comprise a data acquisition controller, said data acquisition controller is connected with said led light source, controls the unlatching of said led light source; Said data acquisition controller also is connected with said optical signal acquisition device, and control said optical signal acquisition device in the schedule time after said led light source is opened or time interval gather said transmittance data; The said schedule time is the time period that 80% required time to said led light source brightness that said led light source brightness rises to peak value drops to 60% required time of peak value.
2. the liquid sample detection device of use led light source according to claim 1 is characterized in that: said optical signal acquisition device is a photovoltaic converter.
3. the liquid sample detection device of use led light source according to claim 1 and 2 is characterized in that: be connected with timing module with said data acquisition controller.
4. the liquid sample detection device of use led light source according to claim 3; It is characterized in that: said data acquisition controller is a single-chip microcomputer; Said single-chip microcomputer is controlled the unlatching of said led light source through electric signal; When said led light source was opened, the said timing module of said Single-chip Controlling picked up counting, and said single-chip microcomputer is controlled said optical signal acquisition device and gathered said transmittance data when said timing module timing reaches the said schedule time or time interval.
5. according to the liquid sample detection device of any described use led light source of claim 1 to 4, it is characterized in that: the said schedule time is that led light source is opened back 2 milliseconds and opened the back 2 seconds time period to led light source.
6. according to the liquid sample detection device of any described use led light source of claim 1 to 4, it is characterized in that: said led light source is that the LED lamp of 260 nanometers and 380 nanometers is formed by sending wavelength.
7. the liquid sample detection device of use led light source according to claim 6 is characterized in that: the light that said LED lamp sends is through synthetic one road light of polariscope.
CN2010101972407A 2010-06-02 2010-06-02 Liquid sample detection device utilizing LED light source Expired - Fee Related CN101871889B (en)

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CN102253009B (en) * 2011-03-17 2012-08-29 河南科技大学 Detection method of edible fungus specimen preserving liquid
CN104281079B (en) * 2014-10-16 2018-02-09 北京北大明德科技发展有限公司 Light transmittance electrical control detecting system and there is its device
CN107976409A (en) * 2017-11-23 2018-05-01 上海海恒机电仪表有限公司 A kind of new online chromascope and its detection method

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