CN102141535A - Enzyme-injection glucose sensing on-line analyzer - Google Patents
Enzyme-injection glucose sensing on-line analyzer Download PDFInfo
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- CN102141535A CN102141535A CN2010106162213A CN201010616221A CN102141535A CN 102141535 A CN102141535 A CN 102141535A CN 2010106162213 A CN2010106162213 A CN 2010106162213A CN 201010616221 A CN201010616221 A CN 201010616221A CN 102141535 A CN102141535 A CN 102141535A
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Abstract
The invention provides an enzyme-injection glucose sensing on-line analyzer for fermentation process, which comprises a flow path structural system 1 of the enzyme-injection glucose sensing on-line analyzer, a three-electrode detection circuit 2, a data acquisition control circuit 3, a liquid crystal screen 4, a personal computer (PC) 5, keys 6 and a power supply 7. By an enzyme-injection mode, the analyzer can realize simultaneous on-line high-pressure steam sterilization of a sensor and a fermentation tank, so that the reliability of the sensor is improved, and detection accuracy is improved; an enzyme exists in a fresh enzyme liquid mode, is independent of detected liquid and is not influenced by the outside, so that frequent calibration of an instrument is not required; different kinds of enzyme liquid are replaced, so that different substances can be detected, namely by one instrument; detection cost is low, and the consumption of liquid to be detected in detection is reduced greatly by a design of dialysis of a sampling probe; and on-line detection of glucose is realized. The analyzer is compact in structure, convenient to process and easy for batch production.
Description
Technical field
The present invention relates to the glucose online measuring technique, is the glucose in-line analyzer of a kind of comprehensive employing physics, chemistry, biology, electronics, specifically, is a kind of enzyme injecting type glucose sensing in-line analyzer towards sweat.
Background technology
The glucose detection technology has a wide range of applications on fermentation industry, food industry, medical analysis of blood sugar, with common glucose analysis method---the fixed sugared method of Fehling, glucose oxidase-peroxidase-colourimetry are compared, glucose biological sensor detects the glucose method, it is strong to have selectivity, be suitable for onlinely, speed is fast, but continued operation, plurality of advantages such as easy and simple to handle has obtained extensive studies and application so glucose biological sensor is surveyed sugar.
Glucose is reactant and intermediate product common in the fermentation process, and the control to concentration of glucose in the sweat can be directly connected to product productive rate and quality.Therefore, the detection of the real-time online of concentration of glucose is very important.Yet in the sweat, in order to prevent microbiological contamination, online glucose biological sensor and and reactor between pipeline must be able to bear high-temperature steam sterilization, to avoid sterilize back sensor performance decline or forfeiture, so concentration of glucose detects the main offline mode that adopts at present, online mode is also at the experimental stage.
At present, glucose sensor adopts the mode of enzyme membrane mostly, yet enzyme membrane is made needs enzyme immobilization technology, is about to glucose oxidase or glucose dehydrogenase is fixed on the semi-permeable diaphragm or on the electrode, the immobilization technology complexity; The activity of enzyme membrane is influenced by test solution, and enzyme membrane reduces gradually with reaction activity, and appliance requires is constantly calibrated; And need bubble to rise before enzyme membrane uses, the time spent is longer, is difficult to online use; A kind of sensor of enzyme membrane can only detect a kind of material; In addition, enzyme membrane costs an arm and a leg.
The present invention is in order to solve the appeal problem, purpose is to provide a kind of and is applicable to onlinely, measures accurately, and is easy to operate, does not need often calibration, the glucose sensing in-line analyzer that cost is low.
Summary of the invention
The objective of the invention is for a kind of enzyme injecting type glucose sensing in-line analyzer towards sweat is provided.
Enzyme injecting type glucose sensing in-line analyzer, it comprises: enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1, three electrode detection circuit 2, data acquiring control circuit 3, liquid crystal display 4, PC 5, button 6, power supply 7, and concrete annexation is:
Described enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1 is connected with data acquiring control circuit 3 with three electrode detection circuit 2, three electrode detection circuit 2 are used for detecting the current signal that enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1 enzyme injecting type glucose biological sensor 8 produces, and data acquiring control circuit 3 is used for providing control signal to enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1; Three electrode detection circuit 2 are connected with data acquiring control circuit 3, and data acquiring control circuit 3 is used to gather the voltage signal of three electrode detection circuit, 2 outputs; Data acquiring control circuit 3 also links to each other with liquid crystal display 4, PC 5, button 6 respectively, liquid crystal display 4 is used for the digital signal of video data acquisition control circuit 3 outputs, PC 5 can communicate by serial ports and data acquiring control circuit 3, and button 6 is used to be provided with data acquiring control circuit 3 operational factors; Power supply 7 links to each other with three electrode detection circuit 2, data acquiring control circuit 3 and enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1, is they power supplies.Do you want three grades of testing circuits are drawn?
Described enzyme injecting type glucose sensing in-line analyzer flow passage structure connected system 1 comprises: enzyme injecting type glucose biological sensor 8, dialysis sampling probe 9, electromagnetism six-way valve 10, quantitatively encircle 11, peristaltic pump 12,13,17, damping fluid bottle 14, enzyme liquid bottle 15, waste liquid bottle 16; Concrete annexation is: described dialysis sampling probe 9 comprises set collar 18, outer supporting network 19, dialysis membrane 20, interior supporting network 21, probe body 22, probe inlet 23, probe outlet 24, diffusion cell 25;
Three electrodes of described enzyme injecting type glucose biological sensor 8 are connected to three electrode detection circuit boards by cable, realize that sensor produces the I/V conversion and the amplification of electric current; The test solution inlet of described enzyme injecting type glucose biological sensor 8 is connected with the probe outlet 24 of dialysis sampling probe 9 by pipeline, and under the work of peristaltic pump 17, the liquid to be measured in the diffusion cell of dialysis sampling probe 9 is introduced in the tested liquid pool; The probe of described dialysis sampling probe 9 inlet 23 is connected with damping fluid bottle 14 by pipeline, and this pipeline passes through peristaltic pump 17;
The 6th port of described electromagnetism six-way valve 10 is connected with damping fluid bottle 14 by pipeline, and this pipeline is through peristaltic pump 12; The 3rd port of described electromagnetism six-way valve 10 is connected with enzyme liquid bottle 15 by pipeline, and this pipeline is through peristaltic pump 13; The 2nd port of described electromagnetism six-way valve 10 is connected with waste liquid bottle 16 by pipeline; The 1st port of described electromagnetism six-way valve 10 links to each other with the 4th port, between connect quantitatively ring 11; The 5th port of described electromagnetism six-way valve 10 is connected with damping fluid, the enzyme liquid inlet of enzyme injecting type glucose biological sensor 8 by pipeline; The damping fluid of described enzyme injecting type glucose biological sensor 8, the outlet of enzyme liquid are connected with waste liquid bottle 16 by pipeline;
Connect the optional stainless steel of the material of this pipeline, teflon, PEEK or silica gel between each parts by pipeline.
Electromagnetism six-way valve 10 constitutes enzyme liquid calibrated shot devices with quantitative ring 11.
Described peristaltic pump the 12,13, the 17th, the peristaltic pump that flow is adjustable, rotating is adjustable.
The optional stainless steel of described probe body 22 materials, PEEK.
Described dialysis membrane 20, optional polycarbonate membrane, polysulfone membrane.
Outer supporting network 19, interior supporting network 21 are made by stainless (steel) wire.
Wherein, dialysis membrane 20 allows glucose molecule or passes through than its little molecule, stops big molecule to pass through.
Advantage of the present invention is:
Adopt enzyme liquid injection system, can realize that sensor carries out online high pressure steam sterilization simultaneously with fermentation tank, not only strengthened the reliability of sensor, and improved the detection degree of accuracy;
Enzyme exists in the enzyme liquid mode of " fresh ", and is independent with test solution, is not subjected to ectocine, makes instrument not need frequent calibration;
Change different types of enzyme liquid, can detect different material, promptly an instrument can detect multiple material;
The detection cost is low, about 0.01 yuan of the used enzyme liquid of single cost;
The design of dialysis sampling probe significantly reduced detect in to the consumption of liquid to be measured;
Realized the online detection of glucose;
The present invention, compact conformation, easy to process is easy to mass production.
Description of drawings
Fig. 1 is the system chart of enzyme injecting type glucose sensing in-line analyzer of the present invention.
Fig. 2 is an enzyme injecting type glucose sensing in-line analyzer flow passage structure connection layout of the present invention.
Fig. 3 is a dialysis sampling probe structure vertical view of the present invention.
Fig. 4 is the cut-open view of dialysis sampling probe structure of the present invention.
Among the figure: 1, enzyme injecting type glucose sensing in-line analyzer flow passage structure system, 2, three electrode detection circuit, 3, data acquiring control circuit, 4, liquid crystal display, 5, computer, 6, button, 7, power supply, 8, enzyme injecting type glucose biological sensor, 9, the dialysis sampling probe, 10, the electromagnetism six-way valve, 11, quantitatively encircle 12, peristaltic pump, 13, peristaltic pump, 14, the damping fluid bottle, 15, enzyme liquid bottle, 16, waste liquid bottle, 17, peristaltic pump, 18, set collar, 19, outer supporting network, 20, dialysis membrane, 21, interior supporting network, 22, probe body, 23, the probe inlet, 24, the probe outlet, 25, diffusion cell.
Embodiment
As shown in Figure 1, enzyme injecting type glucose sensing on-line analysis instrument system provided by the invention comprises as the lower part: enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1, three electrode detection circuit 2, data acquiring control circuit 3, liquid crystal display 4, PC 5, button 6, power supply 7.
Wherein, described enzyme injecting type glucose sensing in-line analyzer flow passage structure system 1 is the flow passage structure part of instrument, connects the optional stainless steel of the material of this pipeline, teflon, PEEK or silica gel between each parts by pipeline.
As shown in Figure 2, enzyme injecting type glucose sensing in-line analyzer flow passage structure connected system 1 comprises following assembly: enzyme injecting type glucose biological sensor 8, dialysis sampling probe 9, electromagnetism six-way valve 10, quantitatively encircle 11, peristaltic pump 12, peristaltic pump 13, damping fluid bottle 14, enzyme liquid bottle 15, waste liquid bottle 16, peristaltic pump 17.
Three electrodes of described enzyme injecting type glucose biological sensor 8 are connected to three electrode detection circuit boards by cable, realize that sensor produces the I/V conversion and the amplification of electric current; The test solution inlet of described enzyme injecting type glucose biological sensor 8 is connected with the probe outlet 24 of dialysis sampling probe 9 by pipeline, and under the work of peristaltic pump 17, the liquid to be measured in the diffusion cell of dialysis sampling probe 9 is introduced in the tested liquid pool; The probe of described dialysis sampling probe 9 inlet 23 is connected with damping fluid bottle 14 by pipeline, and this pipeline passes through peristaltic pump 17;
The 6th port of described electromagnetism six-way valve 10 is connected with damping fluid bottle 14 by pipeline, and this pipeline is through peristaltic pump 12; The 3rd port of described electromagnetism six-way valve 10 is connected with enzyme liquid bottle 15 by pipeline, and this pipeline is through peristaltic pump 13; The 2nd port of described electromagnetism six-way valve 10 is connected with waste liquid bottle 16 by pipeline; The 1st port of described electromagnetism six-way valve 10 links to each other with the 4th port, between connect quantitatively ring 11; The 5th port of described electromagnetism six-way valve 10 is connected with damping fluid, the enzyme liquid inlet of enzyme injecting type glucose biological sensor 8 by pipeline; The damping fluid of described enzyme injecting type glucose biological sensor 8, the outlet of enzyme liquid are connected with waste liquid bottle 16 by pipeline;
Wherein, enzyme injecting type glucose biological sensor 8 has been applied for patent of invention.
Wherein, electromagnetism six-way valve 10 constitutes enzyme liquid calibrated shot devices with quantitative ring 11.
Wherein, dialysis sampling probe 9 is used for inserting liquid to be measured, and liquid to be measured is taken a sample.
Wherein peristaltic pump 12, peristaltic pump 13 and peristaltic pump 17 are peristaltic pumps that flow is adjustable, rotating is adjustable.
As shown in Figure 3, Figure 4, dialysis sampling probe 9 is used for inserting liquid to be measured, liquid to be measured is taken a sample, and it comprises following assembly: set collar 18, outer supporting network 19, dialysis membrane 20, interior supporting network 21, probe body 22, probe inlet 23, probe outlet 24, diffusion cell 25.
Wherein, probe body 22 materials, optional stainless steel, PEEK etc.
Wherein, dialysis membrane 20, optional polycarbonate membrane, polysulfone membrane etc.
Wherein, outer supporting network 19, interior supporting network 21 can be made by stainless (steel) wire.
Wherein, by peristaltic pump 17 rotatings, promote that the test substance in the liquid to be measured is accelerated diffusion in diffusion cell 25.
The enzyme injecting type glucose sensing in-line analyzer course of work of the present invention is as follows:
The sampling probe 9 of will dialysing inserts in the liquid to be measured, opens instrument, and Instrument working parameter is set.Following process will be finished automatically, at first, peristaltic pump 12, peristaltic pump 13 peristaltic pumps 17 start, extract damping fluid or enzyme liquid to electromagnetism six-way valve 10, quantitatively encircle 11, enzyme injecting type glucose biological sensor 8, dialysis sampling probe 9 clean, cleaning finishes, and stops 3 peristaltic pumps.Then, low rate start peristaltic pump 17 makes its step rotating, controls peristaltic pump 17 rotation times diffusion time by setting.Diffusion finishes, and introduces in the tested liquid pool of enzyme injecting type glucose biological sensor 8 by the liquid to be measured that peristaltic pump 17 will diffuse into, and starts enzyme injecting type glucose biological sensor 8 magnetic stirring rotators.After introducing finishes, the soft electromagnetism six-way valve 10 that changes, open peristaltic pump 12, will by electromagnetism six-way valve 10 and quantitatively the ring 11 enzyme liquid calibrated shot devices of forming quantitative enzyme liquid be incorporated in the reaction tank of enzyme injecting type glucose biological sensor 8, in reaction tank, glucose in glucose oxidase and the liquid to be measured reacts, and produces sensor current signal.Three electrode detection circuit, data acquiring control circuit system detect, gather and handle this current signal, obtain the concentration of glucose numerical value of current signal correspondence therewith, this concentration of glucose numerical value can show on liquid crystal display 4, also can pass to PC 5, carry out the processing of host computer by serial ports.
Claims (7)
1. enzyme injecting type glucose sensing in-line analyzer, it is characterized in that: it comprises: enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1), three electrode detection circuit (2), data acquiring control circuit (3), liquid crystal display (4), PC (5), button (6), power supply (7), and concrete annexation is:
Described enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1) is connected with data acquiring control circuit (3) with three electrode detection circuit (2), three electrode detection circuit (2) are used for detecting the current signal that enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1) enzyme injecting type glucose biological sensor (8) produces, and data acquiring control circuit (3) is used for providing control signal to enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1); Three electrode detection circuit (2) are connected with data acquiring control circuit (3), and data acquiring control circuit (3) is used to gather the voltage signal of three electrode detection circuit (2) output; Data acquiring control circuit (3) also links to each other with liquid crystal display (4), PC (5), button (6) respectively, liquid crystal display (4) is used for the digital signal of video data acquisition control circuit (3) output, PC (5) can communicate by serial ports and data acquiring control circuit (3), and button (6) is used to be provided with data acquiring control circuit (3) operational factor; Power supply (7) links to each other with three electrode detection circuit (2), data acquiring control circuit (3) and enzyme injecting type glucose sensing in-line analyzer flow passage structure system (1), is they power supplies;
Described enzyme injecting type glucose sensing in-line analyzer flow passage structure connected system (1) comprising: enzyme injecting type glucose biological sensor (8), dialysis sampling probe (9), electromagnetism six-way valve (10), quantitative ring (11), peristaltic pump (12,13,17), damping fluid bottle (14), enzyme liquid bottle (15), waste liquid bottle (16); Concrete annexation is: described dialysis sampling probe (9) comprises set collar (18), outer supporting network (19), dialysis membrane (20), interior supporting network (21), probe body (22), probe inlet (23), probe outlet (24), diffusion cell (25);
Three electrodes of described enzyme injecting type glucose biological sensor (8) are connected to three electrode detection circuit boards by cable, realize that sensor produces the I/V conversion and the amplification of electric current; The test solution inlet of described enzyme injecting type glucose biological sensor (8) is connected with the probe outlet (24) of dialysis sampling probe (9) by pipeline, under the work of peristaltic pump 16, the liquid to be measured in the diffusion cell of dialysis sampling probe (9) is introduced in the tested liquid pool; The probe inlet (23) of described dialysis sampling probe (9) is connected with damping fluid bottle (14) by pipeline, and this pipeline is through peristaltic pump (17);
The 6th port of described electromagnetism six-way valve (10) is connected with damping fluid bottle (14) by pipeline, and this pipeline is through peristaltic pump (12); The 3rd port of described electromagnetism six-way valve (10) is connected with enzyme liquid bottle (15) by pipeline, and this pipeline is through peristaltic pump (13); The 2nd port of described electromagnetism six-way valve (10) is connected with waste liquid bottle (16) by pipeline; The 1st port of described electromagnetism six-way valve (10) links to each other with the 4th port, between connect quantitatively ring (11); The 5th port of described electromagnetism six-way valve 9 is connected with damping fluid, the enzyme liquid inlet of enzyme injecting type glucose biological sensor (8) by pipeline; The damping fluid of described enzyme injecting type glucose biological sensor 8, the outlet of enzyme liquid are connected with waste liquid bottle (16) by pipeline;
Probe body (22) top of described dialysis sampling probe (9) has probe inlet (23) and probe outlet (24), the bottom of probe body (22) is equipped with interior supporting network (21), bottom outside is fixed with outer supporting network (19), by set collar (18) interior supporting network (21) and outer supporting network (19) is fixed on probe body (22) bottom; Constitute diffusion cell (25) between described probe body (22) and interior supporting network (21); Fixing dialysis membrane (20) between supporting network (21) and outer supporting network (19) in described, interior supporting network (21) and outer supporting network (19) are to dialysis membrane (a 20) reinforcement effect; Described set collar (18) outer ring is threaded, and is threaded with the screwed probe body of an end (22), and externally supporting network (19), dialysis membrane (20), interior supporting network (21) are fixed; Described probe inlet (23) and probe outlet (24) are used for the inflow and the outflow of liquid.
2. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: connect the optional stainless steel of the material of this pipeline, teflon, PEEK or silica gel between each parts by pipeline.
3. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: electromagnetism six-way valve 10 constitutes enzyme liquid calibrated shot devices with quantitative ring 11.
4. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: described peristaltic pump (12,13,17) is the peristaltic pump that flow is adjustable, rotating is adjustable.
5. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: the optional stainless steel of described probe body (22) material, PEEK.
6. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: described dialysis membrane (20), optional polycarbonate membrane, polysulfone membrane.
7. according to the described enzyme injecting type of claim 1 glucose sensing in-line analyzer, it is characterized in that: outer supporting network (19), interior supporting network (21) are made by stainless (steel) wire.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102608342A (en) * | 2012-02-29 | 2012-07-25 | 北京工业大学 | Sampling device for enzyme injection type glucose biosensor online analyzer |
| CN102980921A (en) * | 2012-11-20 | 2013-03-20 | 广东电网公司电力科学研究院 | Detection tank system for interface electrochemical reaction |
| CN103006239A (en) * | 2013-01-08 | 2013-04-03 | 哈尔滨工业大学（威海） | Microsensor for ultrafiltration sampling and glucose detection |
| CN103235016A (en) * | 2013-04-23 | 2013-08-07 | 北京工业大学 | Glucose biosensor for online detection |
| CN106525927A (en) * | 2016-12-13 | 2017-03-22 | 中驭(北京)生物工程有限公司 | Automatic detection apparatus and method for glucose in peritoneal dialysis machine |
| CN112362833A (en) * | 2020-10-28 | 2021-02-12 | 福建省吉龙德环保科技有限公司 | Continuous flow analysis system applied to unmanned ship |
| CN115184441A (en) * | 2022-07-26 | 2022-10-14 | 山东艾科森特生物科技有限公司 | Online detection device and method for multi-channel enzyme electrode in fermentation production process |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608342A (en) * | 2012-02-29 | 2012-07-25 | 北京工业大学 | Sampling device for enzyme injection type glucose biosensor online analyzer |
| CN102980921A (en) * | 2012-11-20 | 2013-03-20 | 广东电网公司电力科学研究院 | Detection tank system for interface electrochemical reaction |
| CN103006239A (en) * | 2013-01-08 | 2013-04-03 | 哈尔滨工业大学（威海） | Microsensor for ultrafiltration sampling and glucose detection |
| CN103235016A (en) * | 2013-04-23 | 2013-08-07 | 北京工业大学 | Glucose biosensor for online detection |
| CN106525927A (en) * | 2016-12-13 | 2017-03-22 | 中驭(北京)生物工程有限公司 | Automatic detection apparatus and method for glucose in peritoneal dialysis machine |
| CN112362833A (en) * | 2020-10-28 | 2021-02-12 | 福建省吉龙德环保科技有限公司 | Continuous flow analysis system applied to unmanned ship |
| CN115184441A (en) * | 2022-07-26 | 2022-10-14 | 山东艾科森特生物科技有限公司 | Online detection device and method for multi-channel enzyme electrode in fermentation production process |
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| CN102141535B (en) | 2013-10-02 |
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