CN102393318A - Simple and quick preparation method of thin-layer liquid-based cytology cell smear - Google Patents
Simple and quick preparation method of thin-layer liquid-based cytology cell smear Download PDFInfo
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Abstract
The invention discloses a simple and quick preparation method of a thin-layer liquid-based cytology cell smear. The method is characterized by comprising the following steps: a, preprocessing a specimen; b, adding 5ml of adhesive separation liquid into a common centrifuge tube; c, centrifuging a sample mixture and discharging the precipitation from the centrifuge tube while reserving the supernate for use; d, placing a glass sheet with a written number into a long slot of a natural settling device fixing seat serving as corollary equipment for film making; e, placing a settling tube on the glass sheet; f, uniformly mixing the bottom sample left after the centrifugation of the step c, and adding the mixture into the settling tube; g, standing still and naturally settling for 15-30 minutes; h, rinsing the specimen with a washing liquid; i, fixing in 95% alcohol for 10 minutes, and performing Papanicolaou staining; and H, performing E staining or immunohistochemical staining and reading the film. Through the simple and quick preparation method of the thin-layer liquid-based cytology cell smear disclosed by the invention, the mucus, blood and inflammatory cells in the specimen can be separated by natural precipitation, the film is clear and the diagnosis accuracy is improved.
Description
Technical field
The present invention relates to a kind of cytolgical examination method; Specifically, be a kind of simple and easy quick liquid-basedcytology cell smear preparation method who is used for ascites pleural fluid, urine, sputum, bronchial brushing sample, gynaecology's health check-up, cervical cancer screening and examination crowd precancerous lesions of uterine cervix etc.
Background technology
Liquid based thin-layer cell detection technique is that a kind of cervical cancer cell advanced in the world today is learned the inspection technology; It has changed the method that conventional cervical smear is taked cell; Put into cell-preservation liquid after the collection of specimens immediately. handle through the automated cell pelleter, through specific gravity liquid centrifugal after, mucus, red blood cell, the inflammatory cell preserved in the liquid are separated; Collect remaining cervical epithelial cells and process the even cell of superthin layer, promptly can be made into the high-quality film-making through HE dyeing in carrying on the glass sheet.The ultra-thin cell preparation of the full-automatic membrane liquid base system's new Bai Shi TCT2000 processor and the supporting special agent cell-preservation liquid (PreserveCyt Solution) thereof of company of U.S. CCID production at present are described as the goldstandard that liquid based cytology detects; But its equipment and reagent cost an arm and a leg, and a people checks about 180 yuan of expense.
Chinese patent CN1967196B discloses a kind of simplified thin-layer liquid basal cell on September 14th, 2006 and has learned the cell smear preparation method, and wherein step comprises: collect that sample, sample place liquid based cell preservative fluid, add the liquid basal cell treating fluid, vibration, centrifuging, get and abandon that supernatant, application of sample rifle are drawn sediment sample, smear, thin slice oven dry, 95% alcohol immersion or spraying is fixing, washing, dye with cervical canal cytobrush and scraper plate.Though it is with low cost to be somebody's turn to do invention, method is easy, is easy to penetration and promotion; Be applicable to the cytolgical examination of gynaecology in enormous quantities generaI investigation, health check-up and basic hospital; Its cheap charge can reduce the patient burden, but it is coated with tablet quality and does not reach the importing technology level, and susceptibility is merely 91.6%.
Therefore known liquid-basedcytology cell smear preparation method exists above-mentioned all inconvenience and problem.
Summary of the invention
The object of the invention; Be to propose preservation liquid, adhesion parting liquid, mucus decomposed solution and the natural subsidence device that a kind of use matches; Difference according to cell proportion; Abandon disturbing the visible component of diagnosing to be isolated in the parting liquid, the cell that letting needs to observe effectively is deposited in the simple and easy quick liquid-basedcytology cell smear preparation method of carrying on the glass sheet.
Another object of the present invention is to propose a kind ofly need not be equipped with expensive support equipment, and is easy and simple to handle, the unrestricted simple and easy quick liquid-basedcytology cell smear preparation method of film-making quantity.
Another purpose of the present invention; Be to propose a kind ofly can meet or exceed the import reagent effect; Reduce medical expense again, the simple and easy quick liquid-basedcytology cell smear preparation method who makes medical resource and cash-starved backcountry also can use up-to-date liquid based thin-layer cell tabletting technology and carry out basic unit in enormous quantities health check-up.
For realizing above-mentioned purpose, technical solution of the present invention is:
A kind of simple and easy quick liquid-basedcytology cell smear preparation method is used for gynaecology's sample, and ascites pleural fluid sample and humoral specimens such as phlegm, urine is characterized in that may further comprise the steps:
A, sample pre-service
Be placed on gynaecology's sample to shake 2～10 minutes on the oscillator together with preserving bottle, draw the middle bottom cell of preserving in the bottle and make sample; Perhaps the ascites pleural fluid sample is abandoned supernatant with the urine humoral specimen after sample is left standstill slightly, 100～150 milliliters in the portion of keeping on file, get behind the mixing 40～50 milliliters with 2000 rev/mins centrifugal 10 minutes, get centrifuge tube bottom 1/3 liquid and add and preserve bottle and make sample; Perhaps sputum specimen adds to be placed on after 5～10 milliliters of the mucus decomposed solution and shakes 2～10 minutes on the oscillator, gets liquid phlegm after the decomposition and adds and preserve bottle and make sample (avoiding drawing the food sediment);
B, get common centrifuge tube and add 5 milliliters and adhere to parting liquids, the back slowly adds through the pretreated equivalent sample of step a sample along centrifugal tube wall;
C, with the sample mixture of step b place hydro-extractor with 1200～2000 rev/mins centrifugal 5～10 minutes, abandon supernatant in the centrifuge tube according to centrifuged deposit thing amount, mixing is for use then;
D, will put into the elongated slot of film-making support equipment natural subsidence device holder with the glass sheet of numbering, glass sheet is close to the elongated slot base of holder, and fixedly back glass sheet frosted end does not exceed the collet outside, damages when preventing film-making;
E, sedimentation pipe one end erect be placed on the glass sheet, sedimentation pipe is rotated in the direction of the clock, the feel sedimentation pipe is tightened and is put in place, and is placed in the middle of the elongated slot of holder;
F, with adding in the sedimentation pipe behind the bottom sample mixing of leaving and taking behind step c centrifugal, if when finding between sedimentation pipe and the glass sheet a little seepage to be arranged, can sedimentation pipe be tightened a little again, up to ne-leakage for extremely;
G, leave standstill natural subsidence after 15～30 minutes, outwell the unnecessary parting liquid liquid in upper strata, sedimentation pipe by rotation counterclockwise, is removed sedimentation pipe, take off glass sheet and get final product;
H, can read the impurity of sheet and wash off speckling with influence on the glass sheet with the washing fluid rinsing the many sample of impurity, range estimation can obviously be seen has a very thin uniform confluent monolayer cells on the glass sheet;
I, dried slightly immediately glass sheet being fixed in 95% alcohol can be used water washing according to the diagnosis needs more than 10 minutes, carried out pap staining again, read sheet behind HE dyeing or the immunohistochemical staining.
Simple and easy quick liquid-basedcytology cell smear preparation method of the present invention can also adopt following technical measures to come further to realize.
Aforesaid method, among the wherein said step a, liquid based cell preservative fluid comprises the formaldehyde and the ethanol of fixed cell effect; The antiseptic sodium Diacetate, anti-coagulants sodium citrate, buffering agent sodium chloride; Potassium chloride and soda mint, the glacial acetic acid of sequestrant EDTA and lysed erythrocyte.
Aforesaid method, wherein the mucus decomposed solution comprises sodium chloride, potassium chloride, lime chloride, soda mint, reducing agent dithiothreitol, formaldehyde, sodium Diacetate and glacial acetic acid.
Aforesaid method among the wherein said step b, adheres to parting liquid and comprises ethanol, sodium chloride, EDTA, soda mint and glacial acetic acid.
Aforesaid method, in the wherein said steps d, film-making support equipment natural subsidence device can use separately, and perhaps a plurality of combinations are used, and combination can be inserted into holder on the fixed head when using, so that operation by the gross.
Aforesaid method, in the wherein said steps d, glass sheet need not carry out coating to be handled.
Aforesaid method, the sample waste liquid in the wherein said operation steps are concentrated and to be put into waste liquid bottle, can inanimate object harmfulness after the neutralization, formaldehydeless discharging.
After adopting technique scheme, simple and easy quick liquid-basedcytology cell smear preparation method of the present invention has the following advantages:
1, can just fix preservation in the very first time, keep cellular morphology, improve accuracy rate of diagnosis isolated preparation;
2, with the cell-preservation liquid of collecting; Through specific gravity liquid centrifugal after, through natural sedimentation the mucus in the sample, blood and inflammatory cell are separated, collecting remaining cell, to process diameter be that 13mm superthin layer cell is in carrying on the glass sheet; Film-making is clear, improves and reads the sheet accuracy;
3, device therefor is simple, and method of operating is convenient, increases substantially work efficiency, and one time how much film-making quantity does not receive place and number of samples quantitative limitation, can need arbitrary combination, fractionation holder according to real work.
4, can repeat film-making, slice-making quality is high, can reach even surmount the level of present import reagent flaking method.
Embodiment
Below further specify the present invention through specific embodiment.
Embodiment 1
Method with preparation of specimen of gynaecology liquid-basedcytology cell smear
A, sample pre-service
Be placed on gynaecology's sample (even preserving bottle) on the oscillator and shook 5 minutes, 10 milliliters in the middle bottom cell in the absorption preservation bottle is got 5 milliliters and is made sample behind the mixing; Liquid based cell preservative fluid comprises the formaldehyde and the ethanol of fixed cell effect, antiseptic sodium Diacetate, anti-coagulants sodium citrate, buffering agent sodium chloride, potassium chloride and soda mint, the glacial acetic acid of sequestrant EDTA and lysed erythrocyte.
B, get common centrifuge tube and add 5 milliliters and adhere to parting liquids, the back slowly adds through the pretreated equivalent sample of step a sample along centrifugal tube wall; Adhere to parting liquid and comprise ethanol, sodium chloride, EDTA, soda mint and glacial acetic acid.
C, with the sample mixture of step b place hydro-extractor with 1500 rev/mins centrifugal 10 minutes, abandon supernatant in the centrifuge tube according to centrifuged deposit thing amount, mixing is for use then;
D, will put into the elongated slot of film-making support equipment natural subsidence device holder with the glass sheet of numbering, glass sheet is close to the elongated slot base of holder, and fixedly back glass sheet frosted end does not exceed the collet outside, damages when preventing film-making; Glass sheet need not carry out coating to be handled; Film-making support equipment natural subsidence device holder can use separately, and perhaps a plurality of combinations are used, and combination can be inserted into holder on the fixed head when using, so that operation by the gross.
E, sedimentation pipe one end erect be placed on the glass sheet, sedimentation pipe is rotated in the direction of the clock, the feel sedimentation pipe is tightened and is put in place, and is placed in the middle of the elongated slot of holder;
F, with adding in the sedimentation pipe behind the bottom sample mixing of leaving and taking behind step c centrifugal, if when finding between sedimentation pipe and the glass sheet a little seepage to be arranged, can sedimentation pipe be tightened a little again, up to ne-leakage for extremely;
G, leave standstill natural subsidence after 30 minutes, outwell the unnecessary parting liquid liquid in upper strata, sedimentation pipe by rotation counterclockwise, is removed sedimentation pipe, take off glass sheet and get final product;
H, can read the impurity of sheet and wash off speckling with influence on the glass sheet with the washing fluid rinsing the many sample of impurity, range estimation can obviously be seen has a very thin uniform confluent monolayer cells on the glass sheet;
I, driedly slightly immediately glass sheet can be used water washing according to the diagnosis needs in fixing 10 minutes in 95% alcohol, carry out again readding sheet behind the pap staining.
Device therefor is simple in the present embodiment method, and method of operating is convenient, increases substantially work efficiency and improves accuracy rate of diagnosis, and film-making is clear, improves and reads the sheet accuracy.
Embodiment 2
Method with preparation of specimen of gynaecology liquid-basedcytology cell smear
A, sample pre-service
Be placed on the oscillator concussion to gynaecology's sample (connect preserve bottle) 5 minutes, abandon supernatant after sample is left standstill slightly, 10 milliliters in the portion of keeping on file gets 5 milliliters and makes sample behind the mixing; Liquid based cell preservative fluid comprises the formaldehyde and the ethanol of fixed cell effect, antiseptic sodium Diacetate, anti-coagulants sodium citrate, buffering agent sodium chloride, potassium chloride and soda mint, the glacial acetic acid of sequestrant EDTA and lysed erythrocyte.It is less to be used for gynaecology's specimen amount, connects to preserve liquid generally at 15～20 milliliters.
B, get common centrifuge tube and add 5 milliliters and adhere to parting liquids, the back slowly adds through the pretreated equivalent sample of step a sample along centrifugal tube wall; Adhere to parting liquid and comprise ethanol, sodium chloride, EDTA, soda mint and glacial acetic acid.
C, with the sample mixture of step b place hydro-extractor with 1800 rev/mins centrifugal 8 minutes, abandon supernatant in the centrifuge tube according to centrifuged deposit thing amount, mixing is for use then;
D, will put into the elongated slot of film-making support equipment natural subsidence device holder with the glass sheet of numbering, glass sheet is close to the elongated slot base of holder, and fixedly back glass sheet frosted end does not exceed the collet outside, damages when preventing film-making; Glass sheet need not carry out coating to be handled; Film-making support equipment natural subsidence device can use separately, and perhaps a plurality of combinations are used, and combination can be inserted into holder on the fixed head when using, so that operation by the gross.
E, sedimentation pipe one end erect be placed on the glass sheet, sedimentation pipe is rotated in the direction of the clock, the feel sedimentation pipe is tightened and is put in place, and is placed in the middle of the elongated slot of holder;
F, with adding in the sedimentation pipe behind the bottom sample mixing of leaving and taking behind step c centrifugal, if when finding between sedimentation pipe and the glass sheet a little seepage to be arranged, can sedimentation pipe be tightened a little again, up to ne-leakage for extremely;
G, leave standstill natural subsidence after 15 minutes, outwell the unnecessary parting liquid liquid in upper strata, sedimentation pipe by rotation counterclockwise, is removed sedimentation pipe, take off glass sheet and get final product;
H, can read the impurity of sheet and wash off speckling with influence on the glass sheet with the washing fluid rinsing the many sample of impurity, range estimation can obviously be seen has a very thin uniform confluent monolayer cells on the glass sheet;
I, driedly slightly immediately glass sheet can be used water washing according to diagnosis needs in fixing 10 minutes in 95% alcohol, carry out again readding sheet after HE dyes.
Device therefor is simple in the present embodiment method, and one time how much film-making quantity does not receive place and number of samples quantitative limitation, can need according to real work, and arbitrary combination, fractionation holder improve and read the sheet accuracy.
Embodiment 3
Method with preparation of specimen of gynaecology liquid-basedcytology cell smear
A, sample pre-service
Be placed on gynaecology's sample (connect and preserve bottle) on the oscillator and shook 10 minutes, get 10 milliliters behind the mixing and make sample; Liquid based cell preservative fluid comprises the formaldehyde and the ethanol of fixed cell effect, antiseptic sodium Diacetate, anti-coagulants sodium citrate, buffering agent sodium chloride, potassium chloride and soda mint, the glacial acetic acid of sequestrant EDTA and lysed erythrocyte.
B, get common centrifuge tube and add 5 milliliters and adhere to parting liquids, the back slowly adds through the pretreated equivalent sample of step a sample along centrifugal tube wall; Adhere to parting liquid and comprise ethanol, sodium chloride, EDTA, soda mint and glacial acetic acid.
C, with the sample mixture of step b place hydro-extractor with 2000 rev/mins centrifugal 5 minutes, abandon supernatant in the centrifuge tube according to centrifuged deposit thing amount, mixing is for use then;
D, will put into the elongated slot of film-making support equipment natural subsidence device holder with the glass sheet of numbering, glass sheet is close to the elongated slot base of holder, and fixedly back glass sheet frosted end does not exceed the collet outside, damages when preventing film-making; Glass sheet need not carry out coating to be handled; Film-making support equipment natural subsidence device holder can use separately, and perhaps a plurality of combinations are used, and combination can be inserted into holder on the fixed head when using, so that operation by the gross.
E, sedimentation pipe one end erect be placed on the glass sheet, sedimentation pipe is rotated in the direction of the clock, the feel sedimentation pipe is tightened and is put in place, and is placed in the middle of the elongated slot of holder;
F, with adding in the sedimentation pipe behind the bottom sample mixing of leaving and taking behind step c centrifugal, if when finding between sedimentation pipe and the glass sheet a little seepage to be arranged, can sedimentation pipe be tightened a little again, up to ne-leakage for extremely;
G, leave standstill natural subsidence after 15 minutes, outwell the unnecessary parting liquid liquid in upper strata, sedimentation pipe by rotation counterclockwise, is removed sedimentation pipe, take off glass sheet and get final product;
H, can read the impurity of sheet and wash off speckling with influence on the glass sheet with the washing fluid rinsing the many sample of impurity, range estimation can obviously be seen has a very thin uniform confluent monolayer cells on the glass sheet;
I, driedly slightly immediately glass sheet can be used water washing according to the diagnosis needs in fixing 10 minutes in 95% alcohol, carry out again readding sheet behind the immunohistochemical staining.
Device therefor is simple in the present embodiment method, can repeat film-making, and slice-making quality is high, can reach even surmount the level of present import reagent flaking method.
Embodiment 4
Make the method for liquid-basedcytology cell smear with sputum specimen
Except that in a sample pre-treatment step, in sputum specimen, adding 5 milliliters of mucus decomposed solution earlier, all the other are with embodiment 2.In the present embodiment method, can be isolated in the visible component of disturbing diagnosis in the parting liquid and abandon the raising slice-making quality.
Embodiment 5
Make the method for liquid-basedcytology cell smear with urine specimen
A, sample pre-service
Urine specimen is abandoned supernatant after sample is left standstill slightly, 150 milliliters in the portion of keeping on file, get behind the mixing 50 milliliters with 2000 rev/mins centrifugal 10 minutes, get centrifuge tube bottom 1/3 liquid and add and preserve bottle and make sample; Liquid based cell preservative fluid comprises the formaldehyde and the ethanol of fixed cell effect, antiseptic sodium Diacetate, anti-coagulants sodium citrate, buffering agent sodium chloride, potassium chloride and soda mint, the glacial acetic acid of sequestrant EDTA and lysed erythrocyte.
B, get common centrifuge tube and add 5 milliliters and adhere to parting liquids, the back slowly adds through the pretreated equivalent sample of step a sample along centrifugal tube wall; Adhere to parting liquid and comprise ethanol, sodium chloride, EDTA, soda mint and glacial acetic acid.
C, with the sample mixture of step b place hydro-extractor with 1500 rev/mins centrifugal 5 minutes, abandon supernatant in the centrifuge tube according to centrifuged deposit thing amount, mixing is for use then;
D, will put into the elongated slot of film-making support equipment natural subsidence device holder with the glass sheet of numbering, glass sheet is close to the elongated slot base of holder, and fixedly back glass sheet frosted end does not exceed the collet outside, damages when preventing film-making; Glass sheet need not carry out coating to be handled; Film-making support equipment natural subsidence device holder can use separately, and perhaps a plurality of combinations are used, and combination can be inserted into holder on the fixed head when using, so that operation by the gross.
E, sedimentation pipe one end erect be placed on the glass sheet, sedimentation pipe is rotated in the direction of the clock, the feel sedimentation pipe is tightened and is put in place, and is placed in the middle of the elongated slot of holder;
F, with adding in the sedimentation pipe behind the bottom sample mixing of leaving and taking behind step c centrifugal, if when finding between sedimentation pipe and the glass sheet a little seepage to be arranged, can sedimentation pipe be tightened a little again, up to ne-leakage for extremely;
G, leave standstill natural subsidence after 20 minutes, outwell the unnecessary parting liquid liquid in upper strata, sedimentation pipe by rotation counterclockwise, is removed sedimentation pipe, take off glass sheet and get final product;
H, can read the impurity of sheet and wash off speckling with influence on the glass sheet with the washing fluid rinsing the many sample of impurity, range estimation can obviously be seen has a very thin uniform confluent monolayer cells on the glass sheet;
I, driedly slightly immediately glass sheet can be used water washing according to the diagnosis needs in fixing 10 minutes in 95% alcohol, carry out again readding sheet behind the immunohistochemical staining.
Device therefor is simple in the present embodiment method, can repeat film-making, and slice-making quality is high, can reach even surmount the level of present import reagent flaking method.
Above embodiment only supplies to explain the present invention's usefulness, but not limitation of the present invention, the technician in relevant technologies field under the situation that does not break away from the spirit and scope of the present invention, can also make various conversion or variation.Therefore, all technical schemes that are equal to also should belong to category of the present invention, should be limited each claim.
Claims (7)
1. a simple and easy quick liquid-basedcytology cell smear preparation method is used for gynaecology's sample, and ascites pleural fluid sample and phlegm, urine humoral specimen is characterized in that may further comprise the steps:
A, sample pre-service
Be placed on gynaecology's sample to shake 2～10 minutes on the oscillator together with preserving bottle, draw the middle bottom cell of preserving in the bottle and make sample; Perhaps the ascites pleural fluid sample is abandoned supernatant with the urine humoral specimen after sample is left standstill slightly, 100～150 milliliters in the portion of keeping on file, get behind the mixing 40～50 milliliters with 2000 rev/mins centrifugal 10 minutes, get centrifuge tube bottom 1/3 liquid and add and preserve bottle and make sample; Perhaps sputum specimen adds to be placed on after 5～10 milliliters of the mucus decomposed solution and shakes 2～10 minutes on the oscillator, gets liquid phlegm after the decomposition and adds and preserve bottle and make sample;
B, get common centrifuge tube and add 5 milliliters and adhere to parting liquids, slowly add through the pretreated above-mentioned equivalent sample of step a sample along centrifugal tube wall;
C, with the sample mixture of step b place hydro-extractor with 1200～2000 rev/mins centrifugal 5～10 minutes, abandon supernatant in the centrifuge tube according to centrifuged deposit thing amount, mixing is for use then;
D, will put into the elongated slot of film-making support equipment natural subsidence device holder with the glass sheet of numbering, glass sheet is close to the elongated slot base of holder, and fixedly back glass sheet frosted end does not exceed the collet outside, damages when preventing film-making;
E, sedimentation pipe one end erect be placed on the glass sheet, sedimentation pipe is rotated in the direction of the clock, the feel sedimentation pipe is tightened and is put in place, and is placed in the middle of the elongated slot of holder;
F, with adding in the sedimentation pipe behind the bottom sample mixing of leaving and taking behind step c centrifugal, if when finding between sedimentation pipe and the glass sheet a little seepage to be arranged, can sedimentation pipe be tightened a little again, up to ne-leakage for extremely;
G, leave standstill natural subsidence after 15～30 minutes, outwell the unnecessary parting liquid liquid in upper strata, sedimentation pipe by rotation counterclockwise, is removed sedimentation pipe, take off glass sheet and get final product;
H, can read the impurity of sheet and wash off speckling with influence on the glass sheet with the washing fluid rinsing the many sample of impurity, range estimation can obviously be seen has a very thin uniform confluent monolayer cells on the glass sheet;
I, dried slightly immediately glass sheet being fixed in 95% alcohol can be used water washing according to the diagnosis needs more than 10 minutes, carried out pap staining again, read sheet behind HE dyeing or the immunohistochemical staining.
2. simple and easy quick liquid-basedcytology cell smear preparation method as claimed in claim 1 is characterized in that, among the said step a; Liquid based cell preservative fluid comprises the formaldehyde and the ethanol of fixed cell effect; The antiseptic sodium Diacetate, anti-coagulants sodium citrate, buffering agent sodium chloride; Potassium chloride and soda mint, the glacial acetic acid of sequestrant EDTA and lysed erythrocyte.
3. simple and easy quick liquid-basedcytology cell smear preparation method as claimed in claim 1 is characterized in that among the said step a, the mucus decomposed solution comprises sodium chloride; Potassium chloride, lime chloride, soda mint; Reducing agent dithiothreitol, formaldehyde, sodium Diacetate and glacial acetic acid.
4. simple and easy quick liquid-basedcytology cell smear preparation method as claimed in claim 1 is characterized in that, among the said step b, adheres to parting liquid and comprises ethanol, sodium chloride, EDTA, soda mint and glacial acetic acid.
5. simple and easy quick liquid-basedcytology cell smear preparation method as claimed in claim 1; It is characterized in that, in the said steps d, film-making support equipment natural subsidence device; Can use separately; Perhaps a plurality of combinations are used, and combination can be inserted into holder on the fixed head when using, so that operation by the gross.
6. simple and easy quick liquid-basedcytology cell smear preparation method as claimed in claim 1 is characterized in that, in the said steps d, glass sheet need not carry out coating to be handled.
7. simple and easy quick liquid-basedcytology cell smear preparation method as claimed in claim 1 is characterized in that, the sample waste liquid in the said operation steps is concentrated and to be put into waste liquid bottle, can inanimate object harmfulness after the neutralization, formaldehydeless discharging.
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