CN102680700A - Quantitative testing reagent for liquid microalbumin in urine and method - Google Patents
Quantitative testing reagent for liquid microalbumin in urine and method Download PDFInfo
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- CN102680700A CN102680700A CN2012101280034A CN201210128003A CN102680700A CN 102680700 A CN102680700 A CN 102680700A CN 2012101280034 A CN2012101280034 A CN 2012101280034A CN 201210128003 A CN201210128003 A CN 201210128003A CN 102680700 A CN102680700 A CN 102680700A
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Abstract
The invention discloses a quantitative testing reagent for liquid microalbumin in urine and a method. The quantitative testing reagent for the liquid microalbumin in urine consists of an R1 reagent and an R2 reagent, wherein the R1 reagent is obtained by dissolving a certain amount of sodium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, sodium azide and polyethylene glycol in distilled water; and the R2 reagent is obtained by dissolving a certain amount of sodium azide in phosphate buffer solution and then adding a certain amount of anti-human albumin antibody into the mixture. The self-developed testing reagent for the liquid microalbumin in urine is combined with an automatic analyzer system to precisely determine the content of microalbumin in urine, so as to provide accurate information for a clinician and possibly test the urine sample quantitatively, automatically and fast.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of microdose urine protein (Urinary Microalbumin) liquid quantitative detectable and method.
Background technology
Urine be by some blood constituents through renal glomerular filtration, renal tubule heavily absorbs, set, excretion form.Clinical uroscopy can directly help the doctor to understand the variation of the uropoiesis physiological function and the pathology function of sufferer, also can reflect the function situation of many internal organs of whole body and system indirectly.Domestic and international clinical urine test is very general, and many and renal function is cardiovascular, and metabolic disease is infected the check in relevant fields such as disease and need be carried out with urine.Conventional urine test comprises following 10: glucose (GLU), cholerythrin (BIL), UBG (URO), ketoboidies (KET), leucocyte (LEC), nitrite (NIT), protein (PRO), potential of hydrogen (pH), occult blood (BLD) and proportion (SG).
Albuminuria (Proteinuria) refers to the illness that contains improper high concentration protein in the urine, and urine protein is mainly albumin, also comprises the protein of some globulin and kidney unit secretion simultaneously.Under normal circumstances, most protein is to get into urine through the filtration of glomerulus, but under the situation that renal function is damaged, some protein can get into urine, forms albuminuria.Urine protein (Urine Protein) and microdose urine protein (Urine Microalbumin) to the albuminuria patient carry out quantitative test, can let the doctor know that patient belongs to microalbuminuria disease (Microalbuminuria) or a large amount of albuminuria disease (Macroalbuminuria).Microalbumin concentration in normal person's urine 2 mg/dL, and microalbuminuria is the early stage disease million of ephrosis, is the important component part of reflection systemic microcirculation, is the index of glomerular capillary permeability, the concentration that it is characterized in that urinary albumin is 2-20 mg/dL; The albumin concentration of a large amount of albuminuria diseases>20 mg/dL, and be in a bad way, often be that hypertension or diabetes cause.The urine test technology of domestic present application mainly is a drying chemical reagent paper qualitative reaction system, can only provide The qualitative analysis as negative or positive, can not be quantitative.Simultaneously, the influenced factor of drying chemical reagent paper method is also more, false negative and false positive can occur inevitably in the clinical position.In addition, the dry chemistry test paper slip receives the restriction of sensitivity, just presents the positive greater than 20 mg/dL, and this moment, renal lesions was irreversible.For ephrosis is carried out early prediction,, carry out the microdose urine protein quantitative testing and have great importance for the clinician provides diagnostic message accurately.
Summary of the invention
Technical matters to be solved by this invention is to remedy the defective that existing dry chemistry reagent system exists, and a kind of microdose urine protein liquid quantitative detectable and method are provided.
It is that a kind of reagent is made up of R1 reagent and R2 reagent that the present invention solves the problems of the technologies described above the technical scheme that is adopted; R1 reagent is prepared by following method: in beaker, add 2-10 g sodium chloride, 1-10 g sodium hydrogen phosphate, 0.1-2.0 g potassium dihydrogen phosphate, 0.1-10 g sodium azide, 10-100 g polyglycol; Be stirred to the Solid-state Chemistry thing with 1L distilled water and dissolve fully, form R1 reagent;
R2 reagent is preferably by following method preparation:
A, with 1L phosphate buffer dissolving 0.1-2.0g sodium azide, be stirred to dissolving fully after, form intermediate solution;
B, adding and intermediate solution volume ratio are the anti-human albumin antibody of 1:20-1:100, form the R2 reagent of homogeneity.
R1 reagent of the present invention is prepared by following method: in beaker, add 4 g sodium chloride, 5.5 g sodium hydrogen phosphates, 1.0 g potassium dihydrogen phosphates, 5g sodium azide, 10 g polyglycol; Be stirred to the Solid-state Chemistry thing with 1L distilled water and dissolve fully, form R1 reagent;
R2 reagent is preferably by following method preparation:
A, with 1L phosphate buffer dissolving 0.5g sodium azide, be stirred to dissolving fully after, form intermediate solution;
B, adding and intermediate solution volume ratio are the anti-human albumin antibody of 1:50, form the R2 reagent of homogeneity.
The microdose urine protein liquid quantitative detection method of any one reagent of the present invention is characterized in that comprising following steps:
A, the R1 reagent of said microdose urine protein liquid quantitative detectable and R2 reagent, put into the R1 and the R2 reagent storehouse of automated analysis appearance after the packing respectively, carry out the parameter setting of microdose urine protein test item then; Sample volume: 2-10ml, R1 reagent: 50-200ml, R2 reagent: 10-100ml, type of calibration: 5AB, wavelength: 340nm, detection method: end-point method, photometry point: 0-30;
B, 5 standard items concentration of setting are respectively 0mg/dL, 1mg/dL, 5mg/dL, 10mg/dL, 30mg/dL, measure the light absorption value of variable concentrations standard items, make the typical curve of concentration to light absorption value;
C, carry out the analysis of reagent detection sensitivity according to typical curve;
D, carry out the precision and the recovery test of sample according to typical curve;
E, carry out the interference analysis test of common drug according to typical curve.
Microdose urine protein liquid quantitative detection method of the present invention, sensitivity reaches 0.16 mg/dL.
Microdose urine protein liquid quantitative detection method of the present invention detects the albumin in the urine specimen, not disturbed by common drug.
Automated analysis appearance of the present invention is Hitachi7180, Olympus5400 or steps auspicious BS-800.
Automated analysis appearance of the present invention is preferably Olympus5400.
The present invention compares with prior art and has the following advantages and effect: this liquid quantitative detectable and method are to utilize the immune response principle; Adopt the liquid reagent system; By the automated analysis appearance microalbumin in the urine is carried out quantitative detection method, have quantitative, accurate, quick and advantages of simple operation simultaneously.The content of microalbumin in can accurately quantitative measurement urine, detection sensitivity reaches 0.16 mg/dL, does not have any cross reaction with common medicine, can diagnostic message accurately and reliably be provided for the clinician.
Description of drawings
Fig. 1 is the typical curve that Olympus5400 automated analysis appearance is measured microdose urine protein.
Embodiment
Below in conjunction with embodiment the present invention is done further detailed description, below invention is to explanation of the present invention and the present invention is not limited to following examples.
Embodiment 1: a kind of preparation process of microdose urine protein liquid quantitative testing reagent:
A, add following chemicals in the beaker: 4 g sodium chloride, 5.5g sodium hydrogen phosphate, 1.0g potassium dihydrogen phosphate, 5g sodium azide, 10 g polyglycol, 1L distilled water is stirred to the Solid-state Chemistry thing and dissolves fully, forms R1 reagent;
B, with 1 L PBS damping fluid dissolving 0.5g sodium azide, be stirred to dissolving fully after, form intermediate solution;
C, adding and intermediate solution volume ratio are the anti-human albumin antibody of 1:50, form the R2 reagent of homogeneity.
Other embodiment 2-13: the preparation process of other embodiment is identical with embodiment 1, and difference only is proportioning, so the proportioning of other embodiment is tabulated as follows:
| Embodiment | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| Sodium chloride (g) | 2 | 10 | 10 | 8 | 4 | 4 | 6 | 6 | 8 | 8 | 2 | 10 |
| Sodium hydrogen phosphate (g) | 1 | 1 | 3.5 | 3.5 | 4.5 | 4.5 | 5.5 | 5.5 | 7.5 | 7.5 | 10 | 10 |
| Potassium dihydrogen phosphate (L) | 0.1 | 0.1 | 0.5 | 0.5 | 1.0 | 1.0 | 1.5 | 1.5 | 1.5 | 2.0 | 2.0 | 2.0 |
| R1 sodium azide (g) | 0.1 | 0.1 | 1 | 1 | 5 | 5 | 7.5 | 7.5 | 9.0 | 9.0 | 9.0 | 10 |
| Polyglycol (g) | 100 | 80 | 70 | 80 | 30 | 30 | 50 | 50 | 10 | 10 | 10 | 20 |
| Antibody and intermediate solution volume ratio | 1：100 | 1：20 | 1：50 | 1：80 | 1：20 | 1：20 | 1：50 | 1：50 | 1：50 | 1：50 | 1：50 | 1：50 |
| R2 sodium azide (g) | 0.1 | 0.1 | 0.5 | 0.5 | 1.0 | 1.5 | 2.0 | 0.5 | 1.0 | 1.5 | 0.1 | 2.0 |
Embodiment 14: a kind of microdose urine protein liquid quantitative detection method; Microdose urine protein liquid quantitative detection method of the present invention is based on a kind of immunoturbidimetry principle; Anti-human albumin antibody in microalbumin in the urine and the reagent forms immune complex; This compound has absorption peak in the 340nm wavelength, can confirm albuminous concentration through the absorption of incident light amount.The concentration of albumin, calibration object and quality-control product in light absorption value size and the sample is directly proportional.
The microdose urine protein liquid quantitative detection method of any one reagent of the present invention comprises following steps:
A, the R1 reagent of said microdose urine protein liquid quantitative detectable and R2 reagent, put into the R1 and the R2 reagent storehouse of automated analysis appearance after the packing respectively, carry out the parameter setting of microdose urine protein test item then; Sample volume: 4ml, R1 reagent: 150ml, R2 reagent: 50ml, type of calibration: 5AB, wavelength: 340nm, detection method: end-point method, photometry point: 10-27.
B, 5 standard items concentration of setting are respectively 0 mg/dL, 1 mg/dL, 5 mg/dL, 10 mg/dL, 30 mg/dL, measure the light absorption value of variable concentrations standard items, make the typical curve of concentration to light absorption value; As shown in Figure 1.
C, carry out the analysis of reagent detection sensitivity according to typical curve; (Limit of detection LOD), has carried out sensitivity experiment for the lowest detection limit value of confirming this microdose urine protein liquid reagent quantitative testing.Its method is following: according to the typical curve of microdose urine protein concentration to light absorption value; The replication portion contains the urine specimen 20 times that microdose urine protein concentration is 0mg/dL; Concentration value and calculating mean value that record obtains; Be designated as
, then LOD=
+3SD.Calculating detection sensitivity through test findings is 0.16mg/dL.
D, carry out the precision and the recovery test of sample according to typical curve; For microdose urine protein liquid reagent quantitative detecting method of confirming to be set up and the check that system can be used for the urine specimen microdose urine protein, carried out the precision and the recovery of sample and tested.Calibration curve and method through microdose urine protein; Replication is blank, the sample of basic, normal, high three concentration (adds the microdose urine protein standard items in the blank urine matrix for each 5 times; Be respectively 0.0,6.0,10.0,30.0 mg/dL to final concentration); Calculate the precision and the recovery, the result is as shown in table 1:
Table 1. precision and recovery test result
| Sample | 1 | 2 | 3 | 4 |
| Sample concentration (mg/dL) | 0.0 | 6.0 | 10.0 | 30.0 |
| test 1 | 0.0 | 5.9 | 9.7 | 29.1 |
| test 2 | 0.0 | 5.7 | 9.7 | 31.2 |
| test 3 | 0.0 | 5.7 | 9.7 | 30.5 |
| test 4 | 0.0 | 5.8 | 9.3 | 31.3 |
| | 0.0 | 5.8 | 9.6 | 29.8 |
| Mean value Mean (mg/dL) | 0.0 | 5.7 | 9.6 | 30.4 |
| Standard SD (mg/dL) | - | 0.1 | 0.2 | 0.9 |
| Recovery Recovery% | - | 96.3 | 96.0 | 101.3 |
| Coefficient of variation CV% | - | 1.4 | 1.8 | 3.1 |
This testing result shows that the system that is set up can be used for the detection of urine sample microdose urine protein, detects the recovery 97.9 ± 3.0%, explains that testing result is accurate; The coefficient of variation (CV%) satisfies the requirement that the liquid quantitative detectable detects microdose urine protein between 1-5, explain that this quantitative detecting method is stable.
E, carry out the interference analysis test of common drug according to typical curve; Choose 46 kinds of common compounds and medicine, adjusting its concentration is 10 mg/dL, carries out interference test and measures, and the result is as shown in table 2:
Table 2.46 kind of common compounds and medicine interference test result
Testing result shows: use result that said microdose urine protein liquid quantitative detection method measures above-claimed cpd all less than 0.2mg/dL, it is noiseless that visible common drug is measured the result to this reagent.
In addition, need to prove, the specific embodiment described in this instructions, its proportioning can be different, allly conceive equivalence or the simple change that described structure, characteristic and principle are done according to patent of the present invention, include in the protection domain of patent of the present invention.Person of ordinary skill in the field of the present invention can make various modifications or replenishes or adopt similar mode to substitute described specific embodiment; Only otherwise depart from structure of the present invention or surmount the defined scope of these claims, all should belong to protection scope of the present invention.
Though the present invention with embodiment openly as above; But it is not in order to limit protection scope of the present invention; Any technician who is familiar with this technology, change and the retouching in not breaking away from design of the present invention and scope, done all should belong to protection scope of the present invention.
Claims (7)
1. microdose urine protein liquid quantitative detectable; It is characterized in that forming by R1 reagent and R2 reagent; R1 reagent is prepared by following method: in beaker, add 2-10 g sodium chloride, 1-10 g sodium hydrogen phosphate, 0.1-2.0 g potassium dihydrogen phosphate, 0.1-10 g sodium azide, 10-100 g polyglycol; Be stirred to the Solid-state Chemistry thing with 1L distilled water and dissolve fully, form R1 reagent;
R2 reagent is prepared by following method:
A, with 1L phosphate buffer dissolving 0.1-2.0g sodium azide, be stirred to dissolving fully after, form intermediate solution;
B, adding and intermediate solution volume ratio are the anti-human albumin antibody of 1:20-1:100, form the R2 reagent of homogeneity.
2. microdose urine protein liquid quantitative detectable according to claim 1; It is characterized in that R1 reagent is prepared by following method: in beaker, add 4 g sodium chloride, 5.5 g sodium hydrogen phosphates, 1.0 g potassium dihydrogen phosphates, 5g sodium azide, 10 g polyglycol; Be stirred to the Solid-state Chemistry thing with 1L distilled water and dissolve fully, form R1 reagent;
R2 reagent is prepared by following method:
A, with 1L phosphate buffer dissolving 0.5g sodium azide, be stirred to dissolving fully after, form intermediate solution;
B, adding and intermediate solution volume ratio are the anti-human albumin antibody of 1:50, form the R2 reagent of homogeneity.
3. microdose urine protein liquid quantitative detection method with claim 1, any one reagent described in 2 is characterized in that comprising following steps:
A, the R1 reagent of said microdose urine protein liquid quantitative detectable and R2 reagent, put into the R1 and the R2 reagent storehouse of automated analysis appearance after the packing respectively, carry out the parameter setting of microdose urine protein test item then; Sample volume: 2-10ml, R1 reagent: 50-200ml, R2 reagent: 10-100ml, type of calibration: 5AB, wavelength: 340 nm, detection method: end-point method, photometry point: 0-30;
B, 5 standard items concentration of setting are respectively 0 mg/dL, 1 mg/dL, 5 mg/dL, 10 mg/dL, 30 mg/dL, measure the light absorption value of variable concentrations standard items, make the typical curve of concentration to light absorption value;
C, carry out the analysis of reagent detection sensitivity according to typical curve;
D, carry out the precision and the recovery test of sample according to typical curve;
E, carry out the interference analysis test of common medicine according to typical curve.
4. microdose urine protein liquid quantitative detection method according to claim 3, it is characterized in that: sensitivity reaches 0.16 mg/dL.
5. microdose urine protein liquid quantitative detectable according to claim 3 is characterized in that: detect the albumin in the urine specimen, not disturbed by common drug.
6. microdose urine protein liquid quantitative detectable according to claim 3 is characterized in that: described automated analysis appearance is Hitachi7180, Olympus5400 or steps auspicious BS-800.
7. microdose urine protein liquid quantitative detectable according to claim 1 is characterized in that: described automated analysis appearance is Olympus5400.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104849473A (en) * | 2015-05-02 | 2015-08-19 | 王贤俊 | Microalbuminuria detection kit and preparation thereof |
| CN105301257A (en) * | 2015-05-02 | 2016-02-03 | 王贤俊 | Detection method for microalbuminuria (mAlb) |
| CN106872718A (en) * | 2017-04-26 | 2017-06-20 | 吉林省富生医疗器械有限公司 | A kind of microdose urine protein detection kit and preparation method thereof |
| CN108982870A (en) * | 2018-07-09 | 2018-12-11 | 山东博科生物产业有限公司 | A kind of microdose urine protein Immunity transmission turbidity detection kit |
| CN109946295A (en) * | 2017-12-20 | 2019-06-28 | 济南腾奔生物技术有限公司 | A kind of microdose urine protein Immunity transmission turbidity detection kit |
| CN114414508A (en) * | 2021-12-30 | 2022-04-29 | 苏州百源基因技术有限公司 | Urine microalbumin detection kit and use method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1576844A (en) * | 2003-07-09 | 2005-02-09 | 松下电器产业株式会社 | Turbidimetric immunoassay and an apparatus therefor |
| US20060154372A1 (en) * | 2004-12-21 | 2006-07-13 | Arter Thomas C | Providing additional motion in assays |
| CN1806252A (en) * | 2003-06-18 | 2006-07-19 | 松下电器产业株式会社 | Vital data vtilization system, method, program and recording medium |
-
2012
- 2012-04-27 CN CN2012101280034A patent/CN102680700A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806252A (en) * | 2003-06-18 | 2006-07-19 | 松下电器产业株式会社 | Vital data vtilization system, method, program and recording medium |
| CN1576844A (en) * | 2003-07-09 | 2005-02-09 | 松下电器产业株式会社 | Turbidimetric immunoassay and an apparatus therefor |
| US20060154372A1 (en) * | 2004-12-21 | 2006-07-13 | Arter Thomas C | Providing additional motion in assays |
Non-Patent Citations (8)
| Title |
|---|
| 《中国现代药物应用》 20090131 余源等 免疫透射比浊法测定微量白蛋白的研究 摘要，第10页左栏第1-9行，右栏第14-19行，第11页第1.2、2.1、2.6节至第12页第2.8节，图6，表1 3-7 第03卷, 第02期 * |
| A HARMOINEN，ET AL: "Turbidimetric measurement of microalbuminuria", 《CLINICA CHIMICA ACTA》 * |
| EM KEARNEY，ET AL: "Simple immunoturbidimetric method for determining urinary albumin at low concentrations using Cobas-Bio centrifugal analyser", 《J. CLIN. PATHOL.》 * |
| LIANG AH,ET AL: "A rapid and sensitive immunoresonance scattering spectral assay for microalbumin", 《CLIN CHIM ACTA》 * |
| 余源等: "免疫透射比浊法测定微量白蛋白的研究", 《中国现代药物应用》 * |
| 张力等: "胶乳透射比浊法检测尿微量白蛋白", 《上海第二医科大学学报》 * |
| 胡汉宁等: "自制尿微量白蛋白试剂盒在生化分析仪上的临床应用", 《陕西医学检验》 * |
| 顾晓琼等: "免疫比浊法测定尿微量白蛋白的探讨", 《现代检验医学杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104849473A (en) * | 2015-05-02 | 2015-08-19 | 王贤俊 | Microalbuminuria detection kit and preparation thereof |
| CN105301257A (en) * | 2015-05-02 | 2016-02-03 | 王贤俊 | Detection method for microalbuminuria (mAlb) |
| CN106872718A (en) * | 2017-04-26 | 2017-06-20 | 吉林省富生医疗器械有限公司 | A kind of microdose urine protein detection kit and preparation method thereof |
| CN109946295A (en) * | 2017-12-20 | 2019-06-28 | 济南腾奔生物技术有限公司 | A kind of microdose urine protein Immunity transmission turbidity detection kit |
| CN108982870A (en) * | 2018-07-09 | 2018-12-11 | 山东博科生物产业有限公司 | A kind of microdose urine protein Immunity transmission turbidity detection kit |
| CN114414508A (en) * | 2021-12-30 | 2022-04-29 | 苏州百源基因技术有限公司 | Urine microalbumin detection kit and use method thereof |
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Application publication date: 20120919 |