CN102712938A

CN102712938A - Method and system for collecting ethanol from aquatic plants

Info

Publication number: CN102712938A
Application number: CN2010800619045A
Authority: CN
Inventors: 托尼·A·哈根
Original assignee: Aquatech Bioenergy LLC
Current assignee: Aquatech Bioenergy LLC
Priority date: 2009-12-01
Filing date: 2010-11-29
Publication date: 2012-10-03
Also published as: JP5852581B2; BR112012013211A2; WO2011068748A2; KR20120106782A; AU2010326162A1; JP2013511998A; EP2507379A2; WO2011068748A3; CA2782367A1; IN2012DN04848A; WO2011068745A3; WO2011068749A2; EP2507379A4; MX2012006299A; WO2011068749A3; IL220039A0; WO2011068745A2

Abstract

Methods and systems for collecting, purifying, and/or extracting ethanol produced during anaerobic metabolism by aquatic plants is provided. The system includes a cell containing water and an aquatic plant, an ethanol extraction assembly in fluid communication with the cell for removing ethanol from the water. Ethanol is released by the aquatic plant by initiating an anaerobic process in the plant such as by regulating the photosynthesis inducing light that reaches the aquatic plant.

Description

Be used for collecting the alcoholic acid method and system from waterplant

Technical field

The disclosure relates to produces and collects the alcoholic acid system and method; And more specifically belong to and be used to promote the plant-growth of plant and the novel method ethanol production of alcohol production, this plant produces free ethanol to form the circulation of keeping certainly (self-sustaining cycle) of plant-growth in the anaerobic metabolism process.The disclosure also relates to collection, purifying and/or extract the alcoholic acid system that waterplant produces in the anaerobic metabolism process.

Summary of the invention

Provide collection, purifying among this paper and/or extract the alcoholic acid method and system that in the anaerobic metabolism process, produces by waterplant.The system that provides in this article has benefited from producing the alcoholic acid method by waterplant, is included in the alternate steps of inducing aerobic and anaerobic metabolism in the plant materials.

Embodiment 1 is that ethanol produces and gathering system, comprises that (compartment cell) with at least a waterplant and the ethanol removal assembly that is communicated with aqueous fluid, and is arranged to suppress the photosynthetic smooth regulation system of waterplant to moisture cell.

Embodiment 2 is that ethanol produces and gathering system, the ethanol removal assembly that comprises moisture cell and at least a waterplant and is communicated with aqueous fluid, and be used to regulate photosynthesis and induce light to arrive the device of at least a waterplant to allow it.Disclosed exemplary means (method) in this article.

Embodiment 3 is the methods of inducing ethanol to form, and this method comprises the steps: to place moisture cell to waterplant; In cell, create anoxia condition, so that cause the Anaerobic processes of waterplant; In cell, create aerobic condition so that cause Aerobic processes; And repeat to create the step of anoxic and aerobic condition.The waterplant size increases and discharges ethanol through the glucide that metabolism in Anaerobic processes stores.Waterplant produces and stores glucide in Aerobic processes.The repeating step of creating anoxic and aerobic condition promotes the waterplant size to increase and improves alcoholic acid to discharge.

In embodiment 4, the method for embodiment 3 further comprises removes the alcoholic acid step from cell.In embodiment 5, embodiment 3 or 4 method further comprise the introducing catalyzer, like 2,4 dichlorophenoxyacetic acid (2,4-dichlorophynoxyacetic acid), to improve the step of anaerobic metabolism.In embodiment 6, the method for any embodiment 3-5 further comprises adding CO ₂Step with the formation that improves aerobic metabolism and glucide.

In embodiment 7, the step of creating the anoxia condition among any embodiment 3-6 is included in the step that has anoxic water in the cell and be included in the creation aerobic condition that has oxygenated water in the cell.In embodiment 8, the anoxic water of maintenance and repeated use embodiment 7 is at least once to cause another time anoxia condition.In embodiment 9, the plant in the method for any embodiment 3-8 can be placed in a large amount of cells, and anoxic water can shift between cell to increase concentration of ethanol in the anoxic water.

In embodiment 10, the method for any embodiment 3-9 further is included in the Aerobic processes to the step of cell introduced plant nutrition with the generation of increase glucide.In embodiment 11, the method for any embodiment 3-10 further comprises the step of the vegetable material that removal is excessive or senesce, and it is used in the biological chemistry industry and supplies with material or sowing (seed) in new cell as waterplant.In embodiment 12, the method for any embodiment 3-11 can use the waterplant in the Potamogetonaceae (family Potamogetonaceae) to implement.

Embodiment 13 is the methods of inducing ethanol to form, and may further comprise the steps: place moisture cell to waterplant; In cell, create aerobic condition to cause the Aerobic processes of plant; Cover cell in case block gas gets in the water with sealing barrier; In cell, create anoxia condition to cause Anaerobic processes; And from water separating alcohol.Waterplant produces and stores glucide in Aerobic processes.The glucide release ethanol that the waterplant size increases and in Anaerobic processes, stores through metabolism.In embodiment 14, the method for embodiment 13 further comprises size increase and the promotion alcoholic acid release of step to promote waterplant that repeats to create anoxic and aerobic condition.

In embodiment 15, embodiment 13 or 14 method are included in further that the coverture with resistance light covers on the cell to prevent that light from getting into the step of cell under the anoxia condition.In embodiment 16, the step that covers on the cell with the coverture that hinders light among the embodiment 15 is defined as the dark stage (dark phase).In embodiment 17, in the aerobic condition process, remove the resistance light coverture among the embodiment 15 or 16, cell is exposed under the light, be defined as photostage (light phase).In embodiment 18, embodiment 16 or dark stage of 17 continued 2 days at least and have time length less than the ratio of 1:2 with respect to the dark stage photostage.

In embodiment 19, the method for any embodiment 13-18 further comprises the introducing catalyzer, like 2,4 dichlorophenoxyacetic acid, to improve the step of anaerobic metabolism.In embodiment 20, the method for any embodiment 13-18 can use the waterplant in the Potamogetonaceae to implement.

In embodiment 21, the method for any embodiment 3-20 further comprises yeast is added the step in the cell.In embodiment 22, the method for any embodiment 3-21 further is included in and produces the step that water stirs in the cell, to prevent under anoxia condition, to adjoin the accumulation of the plant waste of waterplant.

Embodiment 23 is the methods of inducing ethanol to form, and may further comprise the steps: place moisture cell to waterplant; In cell, create aerobic condition so that plant causes Aerobic processes; Cover cell with resistance optical screen barrier and get into cell to prevent light; In cell, create anoxia condition to cause Anaerobic processes; With separating alcohol from water.Waterplant produces and stores glucide in Aerobic processes.The glucide release ethanol that the waterplant size increases and in Anaerobic processes, stores through metabolism.In embodiment 24, the method for embodiment 23 comprises that further the step that repeats to create anoxic and aerobic condition is so that size increase of promotion waterplant and promotion alcoholic acid discharge.

In embodiment 25, the step that using of embodiment 23 or 24 is hindered light coverture covering cell is defined as the dark stage.In embodiment 26, under aerobic condition, remove the resistance light coverture of any embodiment 23-25, so that cell is exposed under the light, be defined as photostage.In embodiment 27, embodiment 25 or dark stage of 26 continued 2 days at least and have time length less than the ratio of 1:2 with respect to the dark stage photostage.

In embodiment 28, the method for any embodiment 23-27 further comprises yeast is added the step in the cell.In embodiment 29, the method for any embodiment 23-28 further comprises the introducing catalyzer, like 2,4 dichlorophenoxyacetic acid, to improve the step of anaerobic metabolism.In embodiment 30, the method for any embodiment 23-39 further is included in and produces the step that water stirs in the cell, with the accumulation of the plant waste that prevents under anaerobic to adjoin waterplant.In embodiment 31, the method for any embodiment 23-30 can use the waterplant in the Potamogetonaceae to implement.In embodiment 32, the method for any embodiment 23-31 further comprises with sealing barrier covering cell in case block gas gets into the step in the water.

Embodiment 33 is the methods of inducing ethanol to form, and this method may further comprise the steps: place moisture cell to waterplant; Causing the supply stage (recharge phase resupplies the stage), is photostage with the water oxygenation to create aerobic condition and plant is exposed to that light gives a definition in this stage; Promote transition phase, make the water anoxic being defined as anaerobic condition, and make cell lack photosynthesis to induce light to be defined as the dark stage in this stage; Through under anoxia condition with in the dark stage, keeping this plant promotion anoxic stage; And catch by plant and be discharged into the ethanol in the water.Promote that plant forms carbohydrate by aerobic metabolism photostage.The dark stage promotes the plant anaerobic metabolism, so that plant is discharged into ethanol in the water.In embodiment 34, promote the step of the transition phase of embodiment 33 further to comprise yeast is added the step in the entry.

In embodiment 35, embodiment 33 or supply stage of 34 maintain between 0.5 day to 12 days.In embodiment 36, the transition phase of any embodiment 33-35 maintains between 0.5 day to 6 days.In embodiment 37, the anoxic stage of any embodiment 33-36 kept 3 days at least.

In embodiment 38, the method for any embodiment 33-37 further comprises the step that repeats to initiate supply stage, promotion transition phase and promote the anoxic stage, discharges to promote ethanol.In embodiment 39, the method for any embodiment 33-38 further is included under the anoxia condition and covers cell to prevent the step in the light entering cell with resistance light coverture, is defined as the dark stage.In embodiment 40, the dark stage of any embodiment 33-39 continued 2 days at least and have time length less than the ratio of 1:2 with respect to the dark stage photostage.In embodiment 41, the method for any embodiment 33-40 further comprises the introducing catalyzer, like 2,4 dichlorophenoxyacetic acid, to improve the step of anaerobic metabolism.In embodiment 42, the method for any embodiment 33-41 further is included in and produces the step that water stirs in the cell, to prevent under anoxia condition, to adjoin the accumulation of the plant waste of waterplant.In embodiment 43, the method for any embodiment 33-42 can use the waterplant of Potamogetonaceae to implement.

In embodiment 44, the dark stage of any embodiment 33-43 continued 2 days and had photostage the time length of the carbohydrate content that must replace losing in the dark stage at least.In embodiment 45, the supply stage of keeping any embodiment 33-44 is to be reconstituted in the glucide that the anoxic stage loses.In embodiment 46, the transition phase of any embodiment 33-45 maintains between 2 days to 6 days.In embodiment 47, the anoxic stage of any embodiment 33-46 kept 6 days at least.

In embodiment 48, promote the step of the transition phase of any embodiment 33-47 to comprise that further yeast, bacterium or enzyme with minimizing oxygen add the step in the entry.In embodiment 49, the method for embodiment 48 further is included in transition phase and adds carbohydrate source to promote the step of anoxia condition.

Thereby the prior characteristics of the disclosure have quite broadly been described, so that can understand its ensuing detailed description better, and so that estimate the contribution of the disclosure better to this area.Will be described below disclosure additional features, and, with the theme that forms the appended claim of the disclosure.

This disclosed part is pointed out and formed to purpose of the present disclosure especially with the various novel features that constitute characteristic of the present disclosure in appended claim.

Embodiment

With reference now to accompanying drawing,, and Fig. 1 and 3 especially, with the novel method ethanol production of describing the principle that embodies embodiment of the present disclosure and notion and be often referred to and be decided to be with reference to figure numbers 20.Fig. 3 is the more detailed synoptic diagram of Fig. 1.Fig. 2,4 and 5 has explained the

various systems

30,40,50 based on method 20.

As shown in figs. 1 and 3, the method 20 of promotion alcohol production and aquatic plants growth is usually included in one or more cells cultivates waterplant.Based on the method that describes below, the system of separating alcohol from waterplant is provided in this article.Can obtain waterplant, and put into cell, as from the lake or plant is collected in the pond, container, cultivate them or directly in cell, cultivate them with any mode commonly used.When implementation method 20, because they need be used for following cell or the purpose that is used to change, so it possibly be used for cultivating and providing waterplant.The type that is used for the water of cell will change based on vegetation type, but fresh water, salt solution and light salt brine (brackish water) all are suitable for various embodiments.

Each cell is configured to deposit water, and maybe or possibly not line up the anti-gentle body of fluid and transfer on the ground surface that supports cell.The specification of cell is for depositing one or more waterplant.The specification of cell will depend on size and type and the needed degree of depth of choosing of waterplant of the waterplant of use, so that suitably growth under hard-core situation.The degree of depth of each cell can about 10cm in the scope of about 20m (for example 10cm to 100cm, 50cm to 1m, 100cm to 1m, 500cm to 3m, 1m to 5m, 4m to 10m, 5m to 7m, 5m to 10m or 10m to 20m).Find that some plants can provide under the situation of other environmental factorss, as have the atypical high water temperature of depths, in the significantly darker degree of depth, grow.For example, shown that Herba Poae Sphondylodis (Stuckenia pectinata) is grown in the depth of water place greater than 20m, the typical hotter water of in this degree of depth place heat through-hole (thermal vents) provides at least than the lake, North America in this degree of depth, being found.

The width of cell and length are inessential to system.Width and the length that should understand cell need not to be equal, and can regulate quantity and type to adapt to the plant of in system, using, and can further depend on profile, available land area, raw-material the obtaining and cost control of cell.When the specification of cell when depositing a plant, this has superiority than the system that comprises a plurality of cells.

Cell also can be temperature control, and especially cell should prevent to kill freezing of waterplant.The heat that is used for cell can be isolated with used heat or other any used heat easily sources that the processing alcoholic acid plant of adjoining discharges.Also can be utilizing extra thermal source under the situation easily, like underground heat and solar heat.In one embodiment, can utilize the water of waste water treatment plant or electric power facility discharging, extra nutrition is provided with attemperation and to waterplant.In addition, in the weather of extreme heat, possibly need the cooling cell will damage plant in addition to prevent temperature.Depend on the various waterplant of utilization, can select TR, it optimizes plant-growth and alcohol production.Optimum growh for example, falls in the scope much wide, in order to maintain the plant (like Herba Poae Sphondylodis) of some selections between 85 ° of Fahrenheit degrees and the 73 ° of Fahrenheit degrees though should be appreciated that the growth and the total temperature scope of alcoholic acid production.A kind of mode of controlled temperature is that cell is sunk in the ground, the temperature that around this place's soil is centered around cell, will regulate cell.

Can be with matrix (substrate), for example thin particle places cell, and waterplant is introduced in the cell, and it can oneself's fixing (grappling) get off in particle at this place.Can use thin particle,, and on the particulate surface, keep the plant material of higher percentage ratio because it can promote the low-yield expenditure of the part that waterplant takes root in particle.

Yet many plants that method 20 is utilized mainly rely on its root system system as fixing means, and therefore can use the mechanism of ammonium fixation or the matrix of any kind of can root growing into.In addition, more intensive particle possibly be useful, and the water flows in cell needs firm anchoring base.Therefore, the cell of the system that provides in this article possibly comprise mechanical fastening system, and like grid or screen cloth, root can be grown into and is connected with the oneself.

Waterplant can be selected from the waterplant of any amount, and it is easy in aquatic environment or on it, survive, as directly at water or in permanent saturated soil.More generally, term " waterplant " can comprise any algae, waterplant or semi-aquatic plant, and it all has height endurability to be submerged in the water or intermittent sinking of the stage of flooding always.And, can in a cell, use polytype waterplant.

Waterplant can comprise, for example, and algae, the pasture and water of underwater; As, but be not limited to Herba Poae Sphondylodis (Stuckenia pectinata) (being called as Herba Potamogetonis Pectinati (Potamogeton pectinatus) in the past), water caltrop (Potamogeton crispus); Herba potamogetonis distincti (Potamogeton distintcus), trifle Herba potamogetonis distincti (Potamogeton nodosus), the climing algae in river (Ruppia maitima), watermifoil (Myriophyllum spicatum); Black algae (Hydrilla certicillata), waterweed (Elodea densa), Herba Hippuridis Vulgaris (Hippuris vulgaris), ruffle grass (Aponogeton boivinianus); Sclerophyll wave grass (Aponogeton rigidifolius), the fringe margin wave grass (Aponogeton longiplumulosus) that comes into leaves, newton's grass (Didiplis diandra), the Java is silk (Vesicularia dubyana) not; Xiao Liu (Hygrophilia augustifolia), pearl Longhairy Antenoron Herb (Micranthemum umbrosum), Da Ai gram grass (Eichhornia azurea), Soviet Union's slave's grass (Saururus cernuus); Tongue green pepper grass (Cryptocoryne lingua), arctic China fir (Hydrotriche hottoniiflora), hundred hakusa (Eustralis stellata), Vallisneria rubra; Willow leaf Hygrophila salicifolia (Hygrophila salicifolia), Thailand's water sword (Cyperus helferi) is trained alizarin green pepper grass (Cryptocoryne petchii), eel grass (Vallisneria americana); Vallisneria torta, arctic China fir (Hydrotriche hottoniiflora), meat leaf grass (Crassula helmsii), Herba Limnophilae sessiliflorae (Limnophila sessiliflora); Wear leaf Herba potamogetonis distincti (Potamogeton perfoliatus), Korean pine tail (Rotala wallichii), Bake green pepper (Cryptocoryne becketii), middle bed mat made of woven strips of bamboo algae (Blyxa aubertii) and water rowland (Hygrophila difformmis); Duckweed as, but be not limited to duckweed (Spirodela polyrrhiza), unrooted duckweed (Wolffia globosa); Article duckweed (Lemna trisulca), the blue or green duckweed of expanding (Lemna gibba), blue or green duckweed (Lemna minor), spot duckweed (Lemna punctata); The water broccoli, as, but be not limited to big duckweed (Pistia stratiotes), buttercup as; But be not limited to Ranunculaceae (Ranunculus), aquatic puncture vine as, but be not limited to water caltrop (Trapa natans) and water caltrop (Trapa bicornis), aquatic lily; Like blue spot water lily (Nymphaea lotus), Nymphaeceae (Nymphaeaceae) and Nelumbonaceae (Nelumbonaceae), aquatic jacinthe as; But be not limited to cloth bag lotus (Eichhornia crassipes), black wood is fainted (Bolbitis heudelotii) and the fringe water shield belongs to (Cabomba); And sea grass as, but be not limited to the little leaf of bamboo (Heterantheta zosterifolia), careless section (Posidoniaceae) is swung in the ripple happiness; Zosteraceae (Zosteraceae), Hydrocharctaceae (Hydrocharitaceae), and Cymodoceaceae (Cymodoceaceae).In addition; In of various embodiments, the host algae can be selected from the group of being made up of green alga, red algae, brown algae, diatom, the living algae in sea, fresh water algae, unicellular algae, many cells algae, sea grass, cold-resistant algae strain, heat-resisting algae strain, the strain of anti-ethanol algae and combination thereof.

Waterplant also can be selected from a kind of in the plant section that comprises Herba potamogetonis distincti (like Stuckenia), Ceratophyllaceae (Ceratophyllaceae), Haloragaceae (Haloragaceae) and Ruppiaceae (Ruppiaceae) usually.More particularly, the waterplant of selection should have bigger Pasteur effect, and it increases anaerobic CO ₂The CO of output and aerobic ₂The ratio of output.Typically, this ratio is about 1:3, but waterplant is like for example Herba Poae Sphondylodis (being called as Herba Potamogetonis Pectinati in the past and also sometimes) and be commonly called Sago Pondweed; Maybe this ratio be increased to 2:1; As " Anoxia tolerance in the aquatic monocot Potamogeton pectinatus:absence of oxygen stimulates elongation in association with an usually large Pasteur effect, ", Journal of Experimental Botany; Volume 51; Number 349, pp1413-1422, and that explains among the August 2000 is the same.Dark with anoxic environment in the elongation process that takes place, the cellular cavity elongation of plant is to be stored in the glucide that aerobic metabolism forms in the waterplant Aerobic processes.Can use these glucide to form ethanol in the anaerobic metabolism process in the Anaerobic processes of waterplant then.Usually, equation is as follows:

Plant aerobic metabolism: 6CO ₂+ 6H ₂O → 6O ₂+ C ₆H ₁₂O ₆

Plant anaerobic metabolism: C ₆H ₁₂O ₆→ 2CO ₂+ 2C ₂H ₅OH

In case waterplant is fixing in cell, in waterplant, causes Anaerobic processes, the glucide that it promotes to store be metabolized to ethanol.In one embodiment, cause or promote Anaerobic processes through in cell, creating anoxia condition (being also referred to as anaerobic condition here).Term " anoxic " is defined as the degree of oxygen depletion here, its inducing plant gets into or keeps the anaerobic metabolism condition under this degree.Therefore, anoxia condition can be enough to reduce or keep the level of oxygen in the vegetable cell, so that promote plant Anaerobic processes or metabolism.

The multiple method that in cell, produces anoxia condition is arranged, and every kind of method can be independently or with the use that combines of one or more additive methods.In one embodiment, create anoxia condition through the oxygen concentration that exhausts or reduce in the water that is included in the cell.This can be incorporated into water through using means strictness organic, chemical or machinery to exhaust in the cell of (for example proposing anaerobic) oxygen and implement.This also can implement through removing oxygen the water in being included in cell.A spot of oxygen should be appreciated that term " anoxic " not necessarily shows there is not oxygen in the water fully, because possibly be dissolved in the water.

This embodiment of the present invention can use a plurality of cells to implement with other embodiments, and wherein anoxic water and oxygenated water circulate between cell on demand, between anoxia condition and aerobic condition, to replace.For example; Utilize the process of a plurality of cells to comprise to contain and reach 2% ethanol (for example 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.75%, 1.0%, 1.25%, 1.5%; Or 1.75%) first cell of anoxic water transfers to before it by in second cell of oxygenation.The oxygenated water that the anoxic water replacement is removed in second cell is so that create anoxia condition in second cell.In second cell, promote plant-growth and alcohol production then.It is pointed out that in second cell, to have ethanol at first (because anoxic water comprises the ethanol from the Anaerobic processes of first cell), when waterplant detects the ethanol in the water, possibly further stimulate alcohol production.For example, in second cell, can allow to increase alcohol concn and reach 4% (for example 0.5%, 0.75%, 1.0%, 1.5%, 1.75%, 2.0%, 2.5%, 3.0%, or 3.5%).When anoxic water being moved in the new cell, just promote elongation and the alcohol production of those plants.In case the alcohol concn in the anoxic water reaches predetermined level,, just anoxic water is shifted out cell and uses habitual means from water, to extract ethanol as for example reaching 10% (for example 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, or 9%) of volume.

Replacedly or in addition; Can in cell, add the additive that reduces oxygen such as cereal, yeast, bacterium (for example being generally the bacterium of hereditary change and/or the bacterium that can ferment); Or enzyme (its consume oxygen and carbohydrate produce carbonic acid gas simultaneously) is with the level of oxygen depleted.In order to promote the level of oxygen depleted, can in water, add originate cereal for example, molasses, wheat or other carbohydrates source of accessory glucide and use for the additive that reduces oxygen.Accessory glucide source can add to cause that thereby enough strong reactions remove a large amount of oxygen from system with yeast.Reduce the ethanol that benefit can be extra production of oxygen through the additive that reduces oxygen.

Lack the Anaerobic processes that enough oxygen promotes waterplant in the water, cause its metabolize carbohydrates and produce ethanol.Can be further through introducing chemical catalyst and CO ₂Promote alcoholic acid production.Suitable chemical catalyst comprises that acetate and 2,4 dichloro benzene ethoxyacetic acid (are commonly called 2,4-d).For example, can obtain CO from waste material source such as electric power facility and oil refinery etc. ₂Can further add extra nutrition and salt, like the salt of potassium, nitrogen and phosphorus, to promote the growth of waterplant.In addition, depend on kind, the organic substrate of the waterplant of utilization, can in cell, add and comprise but be not limited to such as those of sucrose, glucose and acetate.

In Anaerobic processes, the waterplant size can increase and can reach 10 times or more length of its initial length.Here term " size " is interpreted as the growth that comprises the plant volume, and it allows the more substantial glucide of storage.The glucide that elongation provides extra Cytology Lab volume to be formed by waterplant after a while with storage.In Anaerobic processes, waterplant produces ethanol and it is discharged into the extracellular in cell in addition.These ethanol leave in the water of cell and remove until the method through following further exposure then.

These Anaerobic processes can take place one day to several days.Under the situation of Herba Potamogetonis Pectinati (or Herba Poae Sphondylodis), though the longer cycle, maybe be favourable as reaching 14 days to improving output efficiency to greatest extent, six days possibilities are enough altogether.The required time will be depended on many factors, like the size of light diffusion, nutrition utilization ratio, cell, the carbon content of the size of plant, plant variety, water temperature and plant.Can allow plant under anoxia condition, to keep and reach several weeks.Time span confirm to depend primarily on the alcoholic acid maximum production.When the ethanol production of plant reduces to when exceeding actual parameter, can not need it be kept under the anoxia condition.In addition, the pH value that must monitor cell is to prevent water become too acid or too alkali.This can use the calcic buffer compounds (like lime carbonate and calcium chloride or through introducing CO ₂(in alkaline water)) neutralize, still will finally depend on the tolerance of special waterplant species in the cell.

In other embodiment, can allow to arrive amount initiation and/or the promotion Anaerobic processes that photosynthesis of plants is induced light through regulating.Especially, between anaerobic phase, can cell be isolated with promoting photosynthetic light source.Lack illumination and promote Anaerobic processes and alcoholic acid to discharge, and prevent to form oxygen through photosynthesis.Can regulate illumination in cell, to create dark condition through any customary way.Should be appreciated that the term " illumination " that should stop only is applicable to the form of radiation or optical wavelength, it serves as photosynthetic catalyzer and depends on the type of the chemoreceptor that every kind of plant is used.Therefore, the term of Shi Yonging " dark " means a large amount of photosynthetic light frequencies of promotion that lack of expression herein.

Can utilize the various photosynthesis that are used for adjusting (for example selectivity prevention/permission) arrival waterplant to induce the method for light.Such method comprises, for example, barrier, coverture, dome or other containment structures, it serves as the optical screen barrier at least in Anaerobic processes.When no longer expecting to maintain waterplant in the anaerobic condition, these above-mentioned barriers, coverture etc. can be removed.In one embodiment, through people's illuminated with visible light cell, yet it promotes plant " dark " condition.Other suitable inflation method comprise that scattering photosynthesis induces the spectral filter of light.Can use source of artificial light maintenance dark condition and/or when not expecting anaerobic condition, optionally allow photosynthesis.

In further embodiment, can be through covering cells with one or more sealing barriers with adjustments of gas (for example air, oxygen, CO ₂, nitrogen, evaporation ethanol etc.) motion of turnover cell promotes Anaerobic processes.For example, sealing barrier can prevent that unwanted oxygen is incorporated in the cell.Sealing barrier (or extra sealing barrier) also can be used to keep the CO in the cell ₂If, especially with CO ₂Add in the cell.In addition, also can keep high N ₂Level, with in the further dilution water or be trapped in the sealing and cell between any O ₂Sealing barrier will seal cell and be communicated with so that prevent cell and the fluid that adjoins inter gas.This will suppress oxygen and get into cell and will promote Anaerobic processes.Sealing barrier can be translucent barrier, so that promote from photothermal the catching that is used for providing to waterplant light source (naturally and/or artificially).Sealing barrier can or also can not constitute aforesaid resistance optical screen barrier, and it is arranged on the cell and gets into cell so that prevent light in Anaerobic processes.Sealing and resistance optical screen barrier can be processed by conventional material.Yet, should be appreciated that to be arranged on cell space, container, dome or other structures on every side, if it is used for such container, also may be defined as sealing and resistance optical screen barrier.

In one embodiment, the Anaerobic processes of proposing description above prior to, follow or replace with Aerobic processes closely.Through in cell, creating aerobic condition, cause and/or promote the Aerobic processes in the waterplant, it promotes the production and the storage of glucide through waterplant.Can create aerobic condition through the whole bag of tricks, it can use independently or in combination.In one embodiment, oxygenated water is added in the cell, or direct introducing of oxygen is included in the water in the cell.In other embodiment, removing gas shield increases to allow the oxygen concentration in the water naturally.Thereby, can through oxygenated water is introduced in the cell, through remove anoxic water and/or allow the oxygen that water discharges through plant and the gas that is exposed to aerobic in natural oxygenation realize aerobic condition.

In further embodiment; Can be independently or with the use that combines of other embodiments; Waterplant is exposed in the illumination so that induce photosynthesis, and through allowing the aerobic condition (its initiation and/or promote Aerobic processes) in cell to stop Anaerobic processes." illumination condition " can be accomplished through the system that controls the light regulating measure and here discuss.For example, can remove optical screen barrier, coverture or strainer etc., so that allow nature or artificial photosynthesis induces light to arrive waterplant.Replacedly or in addition, can keep the optical screen barrier in the original place and regulate source of artificial light and induce light to arrive waterplant to allow photosynthesis.

In Aerobic processes, can be with waste material, as from the biology material of method 10, trade waste, Municipal waste etc., join in the cell nutrition to be provided to waterplant.In addition, promote to filter maximum sunlight/artificial light to promote the growth of waterplant with temperature regulation.Can strengthen illumination itself through adding artificial light.

Though can usually, continue photostage between 1/2 day and 15 days according to this time frame of particular requirement adjustment of plant, and more generally at least 3 to 6 days, carbohydrate formed to allow waterplant.In addition, the light that can adjust photostage is to provide the photoperiod of fit for service plant species, so that keep the production of plant health and glucide.In Aerobic processes, that kind as noted above, waterplant produces glucide through metabolic process, then glucide is remained in the structure of its elongation.The time length of Aerobic processes depends on a large amount of factors, but when glucide production begins to slow down or reach predetermined level, will stop typically.Using Herba Potamogetonis Pectinati (Herba Poae Sphondylodis), depend on the envrionment conditions in the cell, can be between 2 days and 14 days.

Especially find that controlling illumination condition and dark condition can influence the mode that waterplant produces ethanol and carbohydrate.For example, can make some waterplant stand to continue some days illumination, be defined as photostage, follow hard on the lasting some skies of restriction illumination and be defined as the dark stage to promote anaerobic, to produce the alcoholic acid process.In one embodiment, the initiation of dark condition and anaerobic condition is taken place simultaneously or before or after it soon, preferably each other within 3 days.Shown a kind of plant Herba Poae Sphondylodis, had up to about 6 days photostage, after this, its carbohydrate stably manufactured or reach predetermined optimum level.Term " my god " be defined as 24 hours.This kind of plant has the big dark stage between 2 days and 30 days, and during this period, it possibly get into Anaerobic processes and produce ethanol.Usually, the ratio with the dark stage will be no more than 1:2 and little of 1:10 in photostage, and more common ratio is between the 1:7 at 1:2.Should be appreciated that in illumination and dark stage, can in water, add CO ₂So that promote carbohydrate and alcoholic acid to form.Finally, control above-mentioned illumination and the ability and the ratio described herein in dark stage is not suitable for all waterplant, because certain plants just possibly experience alcohol production after less than 4 hours dark stage.For these types of waterplant, the ratio in photostage and dark stage can be greater than 2:1, though for alcohol production, than the enforcement such as the plant of Herba Poae Sphondylodis, such waterplant possibly have different restrictions.

In case form peak near glucide, or such predeterminated level, just with the re-initiation Anaerobic processes, with the process of beginning carbohydrate metabolism and ethanol formation.Though the process of illustrating above begins with anaerobic stages, aerobic stage and then afterwards, will understand in cell and can at first cause arbitrary stage after waterplant confirmed.Can repeat to create the step of anoxia condition and aerobic condition, constantly to promote elongation and alcohol production, and then glucide production.When plant-growth replenishes plant material that plant senescence lost and no longer satisfies the tolerance of the alcohol production of setting up with those, just formed and supported circulation certainly.Can remove the extra plant-growth that can not be used for replenishing purpose or be used to another cell supply plant materials, and utilize the traditional method fermentation also can be used to produce ethanol.Can catch the carbonic acid gas that discharges during the fermentation, and be recovered in the cell to promote glucide production.Plant waste before or after fermenting process; Can be further be used for to cell extra-nutrition thing and/or can handle being used for the biological chemistry industrial application, as in ethanol and oil motor biofuel, pharmaceuticals, makeup, tinting material, paint etc. as aliment.When finish photostage, between the stage can be arranged in oxygenation stage and anoxic the transitional period, during the amount of oxygen be consumed.During the transitional period, yeast is joined in the cell, it will promote the reduction of oxygen and will allow yeast to produce ethanol that this can be useful.The ethanol that is formed by yeast can serve as the active catalyzer of plant anaerobism, and extra ethanol production output will be provided.The carbohydrate or other glucide that add with yeast can further improve the anaerobism activity.

Defining this three parts circulation more largo comprises: 1) the supply stage; Wherein oxygenation is exposed in the light so that form glucide in water and/or with plant; 2) transition phase wherein makes the water anoxic, makes cell lack photosynthesis and induces light and/or add yeast to form ethanol and oxygen depleted; With 3) the anoxic stage, wherein plant gets into and discharges the alcoholic acid Anaerobic processes.Can stage be defined as second transition phase, wherein allow water to become oxygenation once more.All stages that can improve in this article to be instructed are so that plant-growth and ethanol production maximization.In one approach, the supply stage possibly take place to surpass 0.5-12 days, and then is 0.5-6 days transition phase, and and then it is at least 6 days anoxic stage then, depend on utilization floristics its can increase to above 20 days.In other method, the supply stage possibly take place to surpass 3-6 days, and then is 2-6 days transition phase, and and then it is at least 6 days anoxic stage then, depend on utilization floristics its can increase to above 20 days.

Can take other steps to increase plant-growth and further to promote alcohol production.For example, form, and prevent hydropexis and finally kill waterplant in order to increase ethanol, can use water agitation system constantly agitate water to promote motion through waterplant and near the water it.This prevents that ethanol from providing nutrition with the accumulation of other waste materials that adjoin plant and to plant.Find further that the stirring of water promotes water additive such as zymic to suspend.Agitation system can comprise through any type of wave motion of plant or the water through plant continue flow.Such moisture mobile system can be connected in the circulation loop by fluid, its Jiang Shui be loaded in the pipeline back and before water is got back to cell from water or otherwise directly from cell, remove ethanol.In some embodiments, in such system, when water is outside cell, can in water, add nutrition, microbiotic, O ₂, CO ₂, yeast, or any other needs or desired additive.In addition, circulation loop also can be used for removing O from water ₂, make the water anoxic with before creating anoxia condition so that get back to cell at it.

Find that also the life cycle of control waterplant is can be favourable aspect the life-span that prolongs waterplant.Especially, the life-span of some waterplant (blooms, flower) stops afterwards in those plants maturations.This can possibly ripely stop through the top that cuts off waterplant at it before.Such cut-out will stop some waterplant to arrive WS and maturation.Also can wholely cut away with part and gather in the crops plant, to remove dead plant materials and cell is narrowed down to allow suitable light to be diffused in the cell.Can allow to cut down material is retained in the cell with to cell extra-nutrition thing.

In the time of implementation method 20, possibly produce bacterium and water bloom, it can be controlled through microbiotic, hydrosulfate, high oxygen pressure, algicide, chlorizating agent, uv light exposure and other domestic methods.Yet, find that method 20 produces a large amount of glucide, and monose especially, it promotes growing of bacterium in cell.Given this reason has been found that for concentration that reduces glucide and the purpose that prevents bacteria breed, it is favourable in cell, introducing and producing the alcoholic acid yeast.Replacedly, or with yeast, enzyme or the bacterium combined concentration that also can be used to reduce glucide.Adding the zymic advantageous effects is the growth of ethanol production.The same as Anaerobic processes, the equation that this process is general is C ₆H ₁₂O ₆→ 2CO ₂+ 2C ₂H ₅OH and being well known in the art.Possibly need the displacement yeast, especially after anoxia condition has been set up and kept above about three days, though this depends on the zymic bacterial strain of use.Also can in system, add accessory glucide source to cause that yeast reacts more consumingly.

Fig. 6 has described to be used for to collect alcoholic acid method 100 from yeast.That method 100 is based on method 20 generally and can use described in this

article system

30,40 and 50 to implement.Method 100 is usually included in field planting waterplant in the cell, introduces yeast in the cell, causes anaerobic condition so that promote plant production uncombined carbon hydrate (for example monose), permission yeast to convert the uncombined carbon hydrate into ethanol, and collects the alcoholic acid step.This method 100 can be used as produces and collects alcoholic acid the only resource, or it can be produced with described herein being used for and collection alcoholic acid additive method is united use.

Can use in this article the anaerobic condition in any method initiating method 100 of discussing, comprise stoping photosynthesis to induce light to arrive plant or preventing that oxygen from getting in the water.In some embodiments, cause anaerobic condition through stoping photosynthesis to induce light to arrive plant with stoping in the oxygen entering water.In method 100, can in cell, introduce yeast at one or more time points.

In the embodiment of method 100, this method mainly or all depends on yeast and converts glucide into ethanol, can cut or destroy the release of plant with the glucide that promotes plant further.In some embodiments, can or destroy plant along stem or leaf cutting.In other embodiments, can be at the root cutting plants.Can use any appropriate means to cut or destroy plant.For example, can use and be similar to those waterplant of cutting machine cutting under water that are used to manage subaquatic plant.In some embodiments, under the situation that does not have cutting, can destroy or damage plant, to promote the release of glucide.For example, can use rake, destroy or damage waterplant.

In some embodiments, method 100 comprises the initiation aerobic condition, so that promote the storage of the glucide of plant.Can use method and system described herein aerobic condition in any appropriate time point initiating method 100.For example, can be when the uncombined carbon hydrate have exhausted, produce ethanol at yeast and become efficient when low, or when alcohol concn reaches predeterminated level, cause aerobic condition.The time point that causes aerobic condition can depend on various conditions, like yeast strain (for example alcohol resistance or fermentation efficiency), floristics (for example alcohol resistance, glucide storage efficiency), be used for the equipment of ethanol collection etc.

After aerobic period, can pass through, for example be exposed to cell in nature or the artificial light re-initiation anaerobic condition.In some embodiments, maybe replacement waterplant and/or yeast after aerobic period.In some embodiments, the yeast in the enough fermenting bacterial replacement methods 100 of ability.

Fig. 2,4 and 5 has described to be used to the

system

30,40 and 50 of the method implementing to describe respectively.Should be understood that under the situation of the method for suitably implementing to describe 20, can combine, add, remove or reset the arbitrary component and the aspect of the system of being described 30,40,50.

Fig. 2 has described to be suitable for the system 30 that single cell uses particularly well, also can use with a plurality of cells though should be appreciated that this system.This system 30 generally includes moisture cell 60 and at least one waterplant 61, and the ethanol that is communicated with cell 60 fluids is removed assembly 66.Cell 60 can sink to interior surface or in the structure of container or complete structure of container more than ground of interior volume, part sinking.Though the cell of circulation or loop-type is to promoting that the water motion in the cell 60 is favourable, cell 60 can have any special profile.Though, can move water with the mode of routine because lower powered demand possibly prove that it is favourable utilizing the gravity lifting system.System 30 further comprises one or more sealing barriers 65, and it suppresses gas, like the oxygen and/or the CO of turnover cell ₂, motion.

Utilize photosynthetic smooth regulation system 62, induce light to enter in the cell with permission/prevention photosynthesis optionally.A plurality of light regulating measures about method 20 have been discussed, and any of these means possibly constitute the light regulation system 62 of all or a part.For example, light regulation system 62 can be included in resistance light coverture or the barrier on one or more cells 60.Replacedly or in addition, light regulation system 62 comprises the structure of holding or comprise cell 60.Should understand light regulation system 62 can still not need, and stops all light to reach the plant of system.Exactly, light regulation system 62 can only stop the light with the photosynthesis of plants of inducible system of certain wavelength or intensity.For example, light regulation system 62 can be a strainer, and it only allows not induce photosynthetic wavelength to pass through.Induce the instance of photosynthetic wavelength to comprise wavelength from about 380nm to about 710nm.Depend on the plant that system 30 uses, induce the photosynthetic wavelength region can be wider or narrower, but can confirm to use known method.In one embodiment, sealing barrier 65 constitutes the single structure that possibly maybe can not separate with light regulation system 62.

Light regulation system 62 can be configured to adjustable to allow photosynthesis to induce light at some time points, as during aerobic metabolism or inducing aerobic metabolism, and induces light at other times point prevention photosynthesis, as at anaerobic metabolism or during inducing anaerobic metabolism.For example, light regulation system 62 is removable.In a further embodiment, light regulation system 62 can be an electrodeposited chromium, so that can be through opaqueness or the color that applies current control device.In some embodiments, light regulation system 62 possibly comprise like the source of artificial light 86 that in Fig. 5, shows and induces light and/or do not induce photosynthetic light so that photosynthesis to be provided.Such source of artificial light 86 be can dispose and the certain intensity of desired conditions or the light of frequency spectrum are suitable for emission.For example, at anaerobic metabolism or during inducing anaerobic metabolism, the photosynthesis of plant that source of artificial light 86 can be launched low intensive light or have a system is induced the light outside the light wavelength scope.Likewise, during the plant aerobic metabolism of system was perhaps induced aerobic metabolism, artificial lighting can be launched the light of inducing photosynthetic intensity and wavelength.

Can use the optimum temperuture of thermal source (like heat exchanger 68) with the plant that obtains special waterplant 61 or use.Other suitable thermals source comprise conventional water-heater, geothermal energy resources, solar energy source and used heat from conventional electric power and petroleum installations.Can from cell, extract water out and draw again through the circuit system 67 of sealing with pump 63 and get back in the cell, between cell 60 and ethanol removal assembly, to provide fluid to be communicated with.Closed circuit system 67 can comprise the access port of water, so that under not with the situation of water over-exposure in gas, allows all additives of discussion to water provides above.Replacedly, cell 60 possibly comprise access port.

Ethanol is removed assembly 66 and can be comprised and can and collect alcoholic acid multiple systems and system component from water extraction.In the embodiment of explanation, assembly 66 comprises the air stripper (be also referred to as gas stripper) 64 of one or more functions for ethanol and water are separated.Gas stripper 64 (for example based on the atmosphere of gas stripper-, N ₂-, or CO ₂) be communicated with to catch the ethanol steam with one or more condensing surface 72 fluids, molecular sieve 70 is used for the purifying steam, and/or container 74 is to store ethanol.If expectation also can be used the total evaporator (not shown).Under the situation of anaerobism of not interrupting implementing in the cell and Aerobic processes, assembly 66 allows to remove continuously ethanol.Can further utilize gas stripper 64, to allow CO ₂, nitrogen and nutrition also be incorporated in the water.Draw water get back to cell 60 before, can be exposed in ultraviolet ray and/or the microbiotic, and can add algicide and do not need growing of bacterium and algae to keep in the healthy cell 60.In some embodiments, ethanol storage vessel 74 is replaced to be used to distribute the assembly (not shown) of ethanol to be used to use or transport.

Fig. 4 has described one-piece construction and function and 30 similar systems 40 of system, but comprise two or

more cell

60A, 60B, some or all cell with directly or indirectly be connected during each other fluid is communicated

with.Cell

60A, 60B can connect through any suitable means.In some embodiments, connect two or more cells through common permeable wall.In other embodiment, two or more cells connect through fluid conduit systems.Connection can be separable.For example, two or more cells can connect through the conduit that comprises closable valve 82, so that interrupt two or more cell 60A, the fluid between the 60B is communicated with.In some embodiments, cell 60A or the 60B source of serving as oxygenated water or the anoxic water of

other cells

60A or 60B via fluid conduit systems.

In some embodiments, can implement the closed circuit system 67 of use in the system that is similar to 30,, provide fluid to be communicated with between the 60B to remove assembly 66 and cell 60A at ethanol.As what show,

cell

60A, 60B remove assembly 66 with ethanol and are connected, and remove assembly 66 so that be sent to ethanol from the water among the cell 60B, and remainder water flow back among the cell 60A after the extraction ethanol.In the embodiment of replacement, each

cell

60A and 60B can remove assembly 66 fluids with ethanol independently and be communicated with.

The other assembly that can be used for

system

30,40 or 50 that shows among Fig. 4; Comprise aerator 78; Oxygen removes device 76 (for example vacuum pump), and/or one or morely is used to remove PM for particulate matter, like the strainer 80 of plant materials, matrix and mikrobe (for example yeast or bacterium).The ethanol that in Fig. 4, shows is removed assembly 66, and function can be similar to the assembly about describing among Fig. 2.

Fig. 5 has described system 50, and this system is included in cell 60 and is similar at the ethanol of closed circuit system 67 illustrated in fig. 2 removes the closed circuit system 67 between the assembly 66.This system further comprises having the circulation loop 90 that aerator 78 and/or oxygen remove device 76, so that with pump 63 treating water it is moved in circulation loop 90.In some embodiments, replace oxygen removal device 76 with the source (not shown) of anoxic water, and/or with the source replacement aerator 78 of oxygenated water.Can be used for introducing additive or comprising the assembly that is used for removing oxygen in configuration cycles loop 90 from water.In some embodiments, valve 82 is included in the circulation loop 90, so that adjust the flow velocity and the direction of water in the circulation loop 90.The function of circulation loop 90 also can be the water that stirs in the cell, or can in cell, comprise the water sepn whisking appliance.About the description of system 30, system 50 comprised separately or served as the source of artificial light 86 of light regulation system 62 in combination with optical screen barrier etc. as before.Especially, source of artificial light 86 can provide photosynthesis to induce light in the photoperiod, and/or in the dark cycle, provides non-photosynthesis to induce light.

The ethanol of system 50 is removed assembly and is different from those that in Fig. 2 and 4, explain, because utilize distiller 84 (for example distilling chromatographic column) to replace gas stripper.In the system of any explanation, can utilize distiller and/or gas stripper.For example, in the low relatively place of alcohol concn, can contained gas stripper 64 in the system, and, can comprise distiller 84 in the system in the higher place of alcohol concn.Any position and arbitrary combination that ethanol removal assembly can be included in system remove ethanol to be applicable to from the water of system.In some embodiments, ethanol is removed assembly and is included in a plurality of positions in the system.

In further embodiment, the ethanol of the system of any explanation is removed assembly can be used in combination one or more ethanol absorption property gathering systems separately or with any other assemblies that disclose in this article.Generally speaking, ethanol absorption property gathering system utilizes film or other absorption techniqueses to separate ethanol and water and other tramp materiaies.The instance of such film is " Siftek " film, is made by Vaperma Gas Separation Solutions.

System

30,40,50 can be complete or with the associating of various other systems.For example, can configuration-

system

30,40,50 adjoin and handle alcoholic acid plant or any other used heat of distributing of used heat source easily with separation.In a further embodiment,

system

30,40,50 is connected with sewage treatment equipment, and it typically has the constant source at the water of the equilibrium temperature of about 50 Fahrenheit degrees to about 85 Fahrenheit degrees.Also can be used to waste water from electric power facility.When being connected, can controlling water and waste water heat exchange in the cell 60, or before and after initial wastewater treatment, can waste water directly be used for cell 60 with wastewater source.Except that providing the high temperature to the water source, wastewater source also can have the nutrient concentrations that helps plant-growth.

Be apparent that the various assemblies of

system

30,40,50 described herein can be with various combination implementation methods 20.In addition, can comprise and be used to control water flows, remove particle, monitor and/or keep water parameter (for example pH value), the monitoring alcohol concn, monitor and/or keep the common components of plant parameter, cutting, destruction or removal plant etc.For example, the system 30 that provides among this paper can comprise the assembly such as valve 82, strainer 80, photo-detector and/or survey meter (for example photosynthesis radioactive radiation transmitter), pH meter, skimmer etc.

Embodiment

Embodiment 1: the alcohol production of waterplant

Two Herba Poae Sphondylodis plant that will adhere to stem tuber shift out the storage growth container, and are placed into individually in the test tube with zero(ppm) water that 35mL boils.Comprise the Resazurin telltale in the water to show anoxia condition.These anoxic samples are placed in the Foilpac (foil wraps), induce light to arrive plant to produce dark condition through stoping photosynthesis, it will allow the water oxygenation once more in the plant cell.Then sample is placed in the chamber of malleation nitrogen atmosphere to prevent the outer sample water oxygenation once more of sample chamber.Allow sample in this chamber of 76 Fahrenheit degrees, to cultivate 3 days then.In the 4th day the morning, from each sample, take out the water sample of 2mL, and state university (South Dakota State University) exists to detect alcoholic acid through HPLC (HPLC) analysis in the South Dakota.The HPLC peak value of each sample shows that ethanol exists.

Embodiment 2: light and microbiotic are to the influence of the alcohol production of waterplant

The Herba Poae Sphondylodis plant sample is taken from the lake material of gathering from the South Dakota lake, and places the bottle with the zero(ppm) water that boils, so that only to cover plant anoxia condition is provided.The stainless cylinder of steel that places incubator to seal in eight samples (D5-8, D11 and D14-16) is so that provide dark condition to sample.Remaining sample D1-4, D9-10 and D12-D13 are placed the plastics quart reservoir of cleaning with vent plug.Microbiotic is joined among the sample D9-D16, ethanol conversion is become acetate so that prevent bacterium.Sample is placed the incubator of about 69 Fahrenheit degrees and allows cultivation 7 days.State university analyzes to confirm ethanol and acetic acid concentration through HPLC (HPLC) with the water extraction of each sample and in the South Dakota.

Do not having under the antibiotic dark condition, cultivation comprises four sample D5, D6, D7 and the D8 that alcohol concn is respectively 10.825g/L, 6.817g/L, 7.733g/L and 10.595g/L.Having under the antibiotic dark condition, cultivating sample D11 and D14 that alcohol concn is respectively 6.573g/L and 4.237g/L.In addition, sample D11 does not contain acetate, and sample D14 contains the acetate that concentration is 2.192g/L, has hinted that antibiotic quantity not sufficient is acetate to stop bacterium with ethanol conversion in the sample 14.The sample of in clean container, cultivating does not contain detectable ethanol, has hinted photosynthesis disturb plant sample produce ethanol.Its result is shown in the table 1.

Table 1

Sample	Dark condition	Microbiotic	Acetate (g/L)	Ethanol (g/L)
					D1	-	-	1.332	?0
D2	-	-	1.616	?0
					D3	-	-	0.503	?0
D4	-	-	1.142	?0
					D5	+	-	2.204	?10.825
D6	+	-	2.865	?6.817

Sample	Dark condition	Microbiotic	Acetate (g/L)	Ethanol (g/L)
					D7	+	-	1.420	?7.733
D8	+	-	5.091	?10.595
					D9	-	+	0	?0
D10	-	+	0	?0
					D11	+	+	0	?6.573
D12	-	+	0.863	?0
					D13	-	+	0.749	?0
D14	+	+	2.192	?4.237
					D15	+	+	0.730	?0
D16	+	+	0	?0

Then with reference to top description; Be used for the embodiment optimum dimensional relationship partly that the disclosure allows; The mode, assembly and the application that comprise variation, material, profile, form, function and the operation of size are attainable; Be considered to any technician for this area and be understand with significantly, and embodiment of the present disclosure is intended to comprise those all relation of equivalence of explaining in the accompanying drawings and in specification sheets, describing.

Therefore, aforementioned content is only thought the exemplary of principle of the present disclosure.And; Because those skilled in the art can expect countless improvement and distortion easily; Do not expect to limit the invention in an accurate structure that shows and describe and the operation, therefore, all suitable improvement and distortion of equal value can fall in the scope of the present disclosure.

Description of drawings

When the detailed description of considering subsequently, will understand the present invention better, and except top those purposes of listing, it is obvious that other purposes can become.Such description is with reference to accompanying drawing:

Fig. 1 is the synoptic diagram that promotes the method for alcohol production and aquatic plants growth according to embodiment of the present disclosure.

Fig. 2 is used for from the synoptic diagram of the system of waterplant separating alcohol according to embodiment of the present disclosure.

Fig. 3 promotes the synoptic diagram of the method for alcohol production and aquatic plants growth according to embodiment of the present disclosure.

Fig. 4 is used for from the synoptic diagram of the system of waterplant separating alcohol according to embodiment of the present disclosure.

Fig. 5 is used for from the synoptic diagram of the system of waterplant separating alcohol according to embodiment of the present disclosure.

Fig. 6 is the synoptic diagram that obtains the alcoholic acid method according to the carbohydrate that embodiment of the present disclosure produces from waterplant.

Claims

1. collect the alcoholic acid method for one kind, said method comprises:

At least one waterplant of field planting in moisture cell;

The step that stops photosynthesis to induce light to arrive said at least one waterplant that hockets induces light to arrive the step of said at least one waterplant with permission photosynthesis; And

Collect ethanol the water in being included in said cell.

2. method according to claim 1 wherein, stops photosynthesis to induce the step of light to be defined as the dark stage.

3. method according to claim 2 further is included in the said dark stage and stops the step of oxygen.

4. according to each described method in the aforementioned claim, wherein, the device that stops photosynthetic light prevents that photosynthesis from inducing light to arrive said at least one waterplant between light source and at least one waterplant.

5. according to each described method in the aforementioned claim, wherein, sealing barrier prevents that oxygen from getting in the said water between source of oxygen and said at least one waterplant.

6. according to each described method in the aforementioned claim, wherein, source of artificial light is between said cell and photosynthetic smooth regulation system.

7. according to each described method in the aforementioned claim, further comprise the step that reduces the oxygen concentration in the said water.

8. according to each described method in the aforementioned claim, further comprise the step of anoxic water being introduced said cell.

9. according to each described method in the aforementioned claim, further comprise CO ₂Add the step in the water that comprises in the said cell.

10. in the cell of moisture and at least one waterplant, collect the alcoholic acid method for one kind, said method comprising the steps of:

In said at least one waterplant, cause Anaerobic processes;

Cause the Aerobic processes of said at least one waterplant; And

Collect ethanol in the said water from be included in said cell.

11. method according to claim 10, wherein, the step of said initiation Anaerobic processes comprises the step that stops photosynthesis to induce said at least one waterplant of light arrival.

12. according to claim 10 or 11 described methods, wherein, the step of said initiation Anaerobic processes comprises that prevention oxygen gets into the step in the said water.

13. according to each described method among the claim 10-12, wherein, the said collection alcoholic acid of at least a portion step occurs in during the said Anaerobic processes.

14. according to each described method among the claim 10-13, wherein, the step of said initiation Anaerobic processes is included in the said water and creates anoxia condition.

15. method according to claim 14 wherein, is created said anoxia condition and is comprised in said cell and introduce anoxic water.

16. according to each described method among the claim 10-15, wherein, the step that causes Anaerobic processes comprises and is limited in that scattering of light gets into the step in the said cell in the said Anaerobic processes.

17. collect the alcoholic acid method, may further comprise the steps for one kind:

In moisture cell, cultivate at least one waterplant;

Cause said at least one waterplant and store glucide;

Causing said at least one waterplant is ethanol with the carbohydrate metabolism that stores; And

From said water, collect ethanol.