CN102809551A - Stroboscopic optical image mapping system - Google Patents
Stroboscopic optical image mapping system Download PDFInfo
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- CN102809551A CN102809551A CN2011101455937A CN201110145593A CN102809551A CN 102809551 A CN102809551 A CN 102809551A CN 2011101455937 A CN2011101455937 A CN 2011101455937A CN 201110145593 A CN201110145593 A CN 201110145593A CN 102809551 A CN102809551 A CN 102809551A
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Abstract
The invention provides a stroboscopic optical image mapping system which comprises a control module, a light source module and an image acquisition unit. The control module actuates a delay control to a first pulse signal which has a cycle and consists of a plurality of pulses, so that a delayed pulse signal is formed. The time interval of adjacent pulses of the delayed pulse signal and the cycle are provided with a time difference. The light source provides an incident beam irradiating an organization containing dye. The organization generates a continuous action level signal by the aid of a second pulse signal. The incident beam triggers the dye in the organization to generate a fluorescent beam as intense as the continuous action level signal. The image acquisition unit acquires the fluorescent beam to generate a plurality of fluorescent images according to the delayed pulse signal.
Description
Technical field
The present invention is a kind of optical system, refers in particular to a kind of stroboscopic formula optical imagery image system of utilizing stroboscopic technique.
Background technology
Can produce cell (the excitable cells of excitatory potential in the biosome; Like neurocyte or cardiac muscle cell etc..), potential difference (PD) changes inside and outside the generation cell via the penetrating of ion on the cell membrane, is to keep the suitable critical function of these cell normal physiologicals.The cell that can produce excitatory potential is being born the initiation (triggering action potential) of transmission of iuntercellular information (cell-cell communication) and excitatory potential.But telecommunication is organized in the cell phase composition by excitatory potential, and the research cell membrane potential changes or organizes action potential (action potentials) change and influence the science of the physiological change of biosome, is called electric physiology; If be limited in heart cell or organize then claim for cardiac electrophysiology.Cardiac electrophysiology is research cardiac function and the main pointer of pathology, and the optical imagery image system is one of main instrument of research cardiac electrophysiology, but still has shortcoming to solve in its use of old type optical imagery image system.Old type optical imagery image system mainly contains biosome heart tissue, optical instrument and detection record device.Native system utilizes the stain (Voltage-sensitive dye) of cell membrane current potential sensitivity, during action potential changes, produces cell membrane potential and changes, and the chemical resonant structure of stain is changed, and the fluorescence excitation of stain thereby generation change.Fluorescence signal is through the amplification of optical instrument, by the capturing images unit (for example: CCD) detection record.Existing instrument has its property do not replaced for cardiac electrophysiology stud, and its advantage comprises: (1) utilizes optical scanning, changes with action potential telecommunication in the not direct contact tissue mode collection organization.(2) organize telecommunication to detect than array electrode mode higher pixel.(3) do not receive the telecommunication of extraneous noise undesired signal.But it must have expensive quickly picking image camera and jumbo image file to store hardware; Make existing instrument because of costing an arm and a leg and time and shadow matter resolution limit can't generally be used; So this patent will improve to these shortcomings, thereby the stroboscopic formula optical imagery image system of development a new generation.
As shown in Figure 1, this figure is existing optical imagery image system synoptic diagram.This optical imagery image system 1 mainly contains tissue substance 10, optical instrument 11 and capturing images unit 12.This tissue substance 10 is arranged on the plummer 13, and this optical instrument 11 is made up of light source 110, collimating mirror group 111, spectroscope 112, filter 113 and 114 of lens combination.The system of Fig. 1 utilizes the stain (Voltage-sensitive dye) of cell membrane current potential sensitivity, during action potential changes, produces cell membrane potential and changes, and the chemical resonant structure of stain is changed, and the fluorescence excitation of stain thereby generation change.Fluorescence signal is through the amplification of optical instrument 11, by capturing images unit 12 detection record.Though existing optical imagery image system 1 provides a method that need not contact animal hearts and can observe the electric variation of heart.But this optical imagery image system 1 needs the fast data acquisition speed on hardware, and is therefore high on cost.
In addition; Like US publication US.Pub.No.2008/0188727 a kind of solid broadband spectral device is disclosed in the prior art; It utilizes broadband light that light emitting diode produces on tissue substance, can increase the temperature of tissue substance because the light-source structure design can provide illumination.So this light source can be incorporated into medical detecting device or other is based on detection systems such as optical scattering or fluorescence.In addition, U.S. Pat .Pat.No.6,680,780 a kind of laser interference systems that detect movable object of instruction.In this technology, probe is arranged on the control device, and this probe can produce corresponding moving according to voltage, then laser is divided into reference light and exciting light, and formed thing light and reference light were interfered and the formation interference fringe when exciting light was projected on the object.According to the displacement and the sense of displacement of interference fringe decision object, convert displacement to voltage then then, and then make this probe produce displacement according to this voltage.
Summary of the invention
The present invention provides a kind of stroboscopic formula optical imagery image system; It utilizes the mechanism of stroboscopic and delay; Capture respectively about a tissue substance under an excited state, corresponding different time delays point fluoroscopic image, by Flame Image Process and filtering calculation with this many corresponding different time delays point fluoroscopic images change and reassemble into; Can represent the processing signals sequence under this excited state, to judge the whether normal foundation of this tissue substance as follow-up.
The present invention provides a kind of stroboscopic formula optical imagery image system; It utilizes the mechanism of stroboscopic and delay; Can needn't use expensive capturing images unit promptly can be fast, do not destroy, contact tissue mode not, tissues observed electricity physiology changes, importantly this technology provides sensitive and high-resolution image quality; Can obtain high-resolution fluoroscopic image, in order to follow-up calculation and signal Processing.
In one embodiment; The present invention provides a kind of stroboscopic formula optical imagery image system; It includes: a control module; It postpones control to form a delayed pulse signal to having one-period and being undertaken one by one first pulse signal that a plurality of pulse constituted, and the time interval of the adjacent pulse that this delayed pulse signal had and this cycle have a mistiming; One light source module; It provides an incident light to shine on a tissue substance that contains a stain; This tissue substance produces a continuous action electric potential signal by one second pulse signal, and this incident light excites this stain in this tissue substance to produce a fluorescence that should continuous action electric potential signal intensity; And a capturing images unit, it couples with this control module mutually, and this capturing images unit forms many fluoroscopic images according to this this fluorescence of delayed pulse signal acquisition.
In another embodiment; The present invention provides a kind of stroboscopic formula optical imagery image system; It includes: a control module; It postpones control to form a delayed pulse signal to having one-period and being undertaken one by one first pulse signal that a plurality of pulse constituted, and the time interval of the adjacent pulse that this delayed pulse signal had and this cycle have a mistiming; One light source module; It couples with this control module mutually; To receive this delayed pulse signal; This light source module of the trigger action of this delayed pulse signal produces an incident light and shines on a tissue substance that contains a stain, and this tissue substance produces a continuous action electric potential signal by one second pulse signal, and this incident light excites this stain in this tissue substance to produce a fluorescence that should continuous action electric potential signal intensity; And a capturing images unit, its this fluorescence of acquisition and form many fluoroscopic images.
Description of drawings
Fig. 1 is existing optical imagery image system synoptic diagram;
Fig. 2 A is the stroboscopic formula optical imagery image system first embodiment configuration diagram of the present invention;
Fig. 2 B is the stroboscopic formula optical imagery image system second embodiment configuration diagram of the present invention;
Fig. 2 C is stroboscopic formula optical imagery image system the 3rd an embodiment configuration diagram of the present invention;
Fig. 2 D suppresses synoptic diagram on tissue substance for the present invention utilizes pressing assembly;
Fig. 3 A is the first pulse signal synoptic diagram;
Fig. 3 B is depicted as the delayed pulse signal synoptic diagram;
Fig. 4 A is that delayed pulse signal and the tissue substance continuous action electric potential signal that produces that is stimulated concerns synoptic diagram;
Fig. 4 B is an electric potential signal reorganization synoptic diagram of the present invention;
Fig. 4 C is that delayed pulse signal of the present invention and continuous action electric potential signal concern another embodiment synoptic diagram;
Fig. 5 A and Fig. 5 B are these many fluoroscopic images and original second a pulse signal synoptic diagram about the specific region on this tissue substance;
Fig. 5 C and Fig. 5 D are respectively signal reorganization embodiment synoptic diagram of the present invention;
Fig. 6 A to Fig. 6 C is that spatial filtering of the present invention is handled the image synoptic diagram;
Fig. 7 A and Fig. 7 B are these many formed after treatment burst synoptic diagram of handling in the image in a specific region;
Fig. 8 is a processing signals sequence synoptic diagram.
Wherein, Reference numeral:
1-optical imagery image system
The 10-tissue substance
The 11-optical instrument
The 110-light source
111-collimating mirror group
The 112-spectroscope
The 113-filter
The 114-lens combination
12-capturing images unit
The 13-plummer
2-stroboscopic formula optical imagery image system
The 20-control module
The 200-controller
201-first delay cell
202-second delay cell
The 21-signal generation device
The 22-plummer
The 23-light source module
24-capturing images unit
The 25-tissue substance
The 250-specific region
26-color separation spectroscope
27,28-wave length filtering sheet
The 29-pressing assembly
90-first pulse signal
The 900-pulse
The 91-delayed pulse signal
The 910-pulse
97-continuous action electric potential signal
970-action potential ripple
The 92-electric potential signal of recombinating
93,93a, 93b-image
The 930-position
The 931-image-region
The 94-burst
The 940-signal
The 95-electric potential signal of recombinating
96-processing signals sequence
Embodiment
For making the auditor further cognition and understanding arranged to characteristic of the present invention, purpose and function; The hereinafter spy describes the relevant thin portion structure of device of the present invention and the theory reason of design; So that the auditor can understand characteristics of the present invention, specify statement as follows:
See also shown in Fig. 2 A, this figure is the stroboscopic formula optical imagery image system first embodiment configuration diagram of the present invention.In the present embodiment, this stroboscopic formula optical imagery image system 2 includes a control module 20, a signal generation device 21, a plummer 22, a light source module 23 and a capturing images unit 24.This control module 20, it carries out one to one first pulse signal and postpones control to form a delayed pulse signal.See also shown in Fig. 3 A, this figure is the first pulse signal synoptic diagram.This first pulse signal 90 has one-period T and is made up of 900 of a plurality of pulses.See also shown in Fig. 3 B, this figure is the delayed pulse signal synoptic diagram.In order to reach the effect of stroboscopic acquisition image; 20 pairs of these first pulse signals 90 of this control module postpone to control the triggered time with each pulse of adjusting this first pulse signal, and time interval Δ T and this cycle T of the adjacent pulse 910 that the delayed pulse signal 91 that makes delay control back produced is had have a mistiming Δ t.
In Fig. 3 B, can find out at the 0th time point t
0The time, trigger formed pulse after, make at the 1st time point t via postponing control
1Pulse that is triggered and the 0th time point t
0Pulse between time interval Δ T and this cycle T between have a mistiming Δ t.When to the n time point, equal this cycle length during T by the 1st to the mistiming that the n time point is added up, when the time point of n+1, then get back to like t again
0The trigger pulse state of time point is periodically analogized with this.
Return shown in Fig. 2 A, in the present embodiment, this control module 20 includes a controller 200 and one first delay cell 201.This controller 200 provides this first pulse signal.This controller 200 can for be integrated with signal produce function have calculation process can computing machine, workstation or server, but not as limit.For example: the mode that the computing machine of operation processing function and signal generator branch are arranged.This first delay cell 201; It couples with this controller 200 and this capturing images unit 24 mutually; 201 pairs of these first pulse signals of this first delay cell carry out this delay control, make the interpulse time span that has and the recurrence interval of adjacent different time points have the mistiming.Separate with this controller 200 in the embodiment of Fig. 2 A though be noted that this first delay cell 201, in another embodiment, can also this first delay cell 201 be combined to form single control module with this controller 200.This is that those skilled in the art can spirit according to the present invention change.
In the present embodiment, this signal generation device 21 produces one second pulse signal.This plummer 22, it provides and carries a tissue substance 25.This tissue substance 25 can be biological tissue, for example: nerve fiber, musculature or heart tissue.In the present embodiment, this tissue substance 25 is a heart tissue.This tissue substance 25 contains a suppressant and a stain, and this tissue substance 25 couples to receive this second pulse signal with this signal generation device 21 mutually.When this tissue substance 25 receives this second pulse signal, can produce a continuous action electric potential signal.This stain belongs to uses the stain (Voltage-sensitive dye) responsive to the cell membrane potential of heart cell, and this stain can be chosen as (di-4-ANEPPs), but not as limit.Owing to when this tissue substance 25 receives this second pulse signal, can produce vibration, so its vibration of this suppressant in order to suppress to be produced when this tissue substance 25 receives this second pulse signal because of the pulse of second pulse signal.In the present embodiment, this suppressant is Cytochalasin D (Cyto D), but not as limit.Be noted that and suppress this tissue substance 25 because the means of the vibration that this second pulse signal of reception is produced can also be used pressing assembly 29 except aforementioned to tissue substance 25 injecting inhibitors, shown in Fig. 2 D.In the present embodiment, this pressing assembly 29 is suppressed on tissue substance to avoid tissue substance to produce vibration for slide.
This light source module 23, it provides an incident light that is complementary with this stain to shine on this tissue substance 25, and this incident light excites this stain in this tissue substance to produce a fluorescence that should continuous action electric potential signal intensity.In the present embodiment, this light source module 23 is a light emitting diode (light emitting diode, a LED) light source module.Because this incident light needs and this stain coupling, just can excite stain to produce fluorescence, therefore in the present embodiment, this incident spectrum is its top efficiency wavelength with 475nm, and the fluorescence excitation wavelength is 617nm.The long selection of efficient filtering that is noted that this incident light decide according to the stain kind, so is not restriction with aforesaid embodiment.
This capturing images unit 24, it couples with this control module 20 mutually, and this capturing images unit 24 forms many fluoroscopic images about this tissue substance 25 according to this this fluorescence of delayed pulse signal acquisition.See also shown in Fig. 3 B, in Fig. 3 B, when each pulse in the delayed pulse signal produced, this capturing images unit 24 was fluoroscopic image of fechtable.This capturing images unit 24 can be chosen as Charged Coupled Device (Charge-coupled Device; CCD) capturing images unit or complementary metal-oxide layer-semiconductor (Complementary Metal-Oxide-Semiconductor, capturing images unit CMOS).It is the capturing images unit that present embodiment is selected CCD.In addition, be noted that to have more a color separation spectroscope 26 and wave length filtering sheet 27 and 28 between this capturing images unit 24 and this tissue substance 25, be the best and avoid CCD to receive the stray light beyond the fluorescence excitation to guarantee wavelength efficiency.
Except stretching frame structure shown in Fig. 2 A, shown in Fig. 2 B, this figure is the stroboscopic formula optical imagery image system second embodiment configuration diagram of the present invention.In the present embodiment, the difference with Fig. 2 A is that this control module 20 more provides this delayed pulse signal to this light source module 23.Make this light source module 23 produce the Frequency Synchronization of incident light frequency and this capturing images unit 24 acquisition images.See also shown in Fig. 2 C, this figure is stroboscopic formula optical imagery image system the 3rd an embodiment configuration diagram of the present invention.Framework in the present embodiment, basically with Fig. 2 category-A seemingly, difference be that delayed pulse signal that the control module 20 of present embodiment is produced only offers this light source module 23 and postpones control.
Utilize after many fluoroscopic images of system's acquisition of Fig. 2 A to Fig. 2 C, 20 pairs of these many fluoroscopic images of this control module carry out image and signal Processing.The principle of image of the present invention and signal Processing is described at first, is seen also shown in Fig. 4 A that this figure is that delayed pulse signal and the tissue substance continuous action electric potential signal that produces that is stimulated concerns synoptic diagram.In order to save system cost, the capturing images unit that employed capturing images unary system is a low speed in the embodiment of the invention.To be stimulated and to obtain the potential change that fluorescence is had after changing in order to access about this tissue substance.The present invention utilizes the pulse signal of out of phase to capture the fluorescence information of diverse location, utilizes the mode of information reorganization to form one group of new data again, utilizes this group new data to judge whether tissue substance has unusually.In Fig. 4 A, when on behalf of tissue substance, label 97 receive second pulse signal, a continuous action electric potential signal that is produced, this continuous action electric potential signal is made up of 970 in a plurality of action potential ripples; And the delayed pulse signal of label 91 representatives through postponing to handle, it is made up of 910 of a plurality of pulses.
In the present embodiment, 970 pairs in each action potential ripple should have a pulse 910.When each pulse 910 produced, the capturing images unit promptly can produce a fluoroscopic image.Though the picture position about this continuous action electric potential signal 97 that each pulse institute driven image acquisition unit is captured is different, can be with the pairing electric potential signal s of the image that different time points captured after the process beol
0～s
7Reorganization is to form new reorganization electric potential signal 92, shown in Fig. 4 B.That is to say that in the present embodiment, the reorganization electric potential signal 92 shown in Fig. 4 B is for to utilize the information combination of 8 second pulse signal gained to form.To carrying out so repeatedly, also can produce about one group of this signal new reorganization electric potential signal.Be noted that in addition among Fig. 4 A and Fig. 4 B embodiment showed is the corresponding pulse of each action potential ripple.In another embodiment, shown in Fig. 4 C, 970 a plurality of pulses of correspondence of each action potential ripple.The general practice can be used than pulse signal faster and form the state like Fig. 4 C, and quantity that will utilize sampling to form a needed action potential ripple of reorganization electric potential signal thus can reduce, that is can increase the efficient of reorganization.
Next the mode that control module is carried out is described, is seen also shown in Fig. 5 A and Fig. 5 B that this figure is these many fluoroscopic images and the electric potential signal synoptic diagram of recombinating about one of the specific region on this tissue substance.Many the fluoroscopic images 93 that in Fig. 5 A, captured for this capturing images unit.Each each pixel of opening in the fluoroscopic image is a different ad-hoc location on this tissue substance of representative.And among Fig. 5 B, then for fluoroscopic image that an ad-hoc location on should tissue substance is had through resulting electrical signal sequence synoptic diagram after the conversion.Be depicted as example with Fig. 5 A, on the ad-hoc location 930 of the ad-hoc location on the tissue substance in fluoroscopic image.Through changing formed reorganization electric potential signal shown in Fig. 5 B.Shown in Fig. 5 C and Fig. 5 D, this figure is respectively signal reorganization embodiment synoptic diagram of the present invention.In the embodiment of Fig. 5 C, reorganization electric potential signal 92 is combined for a plurality of action potential ripples 970 (present embodiment is four, and the action potential wave number amount of its reorganization institute palpus is decided as required, does not exceed with these four).Adjacent reorganization electric potential signal 92 then differs 970 elapsed times of four action potential ripples.And in Fig. 5 D, belong to a kind of signal recombination form of real-time reorganization electric potential signal.That is to say; When the reorganization electric potential signal 92 when first is accomplished reorganization; As long as again through 970 elapsed times of an action potential ripple; Promptly can be again by previous reorganization electric potential signal 92 needed last three the action potential ripples of reorganization, and reassemble into a new reorganization electric potential signal again, and then reach the effect of real-time reorganization.
Can find out that by Fig. 5 B the formed pulse potential signal in process reorganization back receives very big noise, therefore is not easy to judge.Therefore, this control module of the present invention more receives this many fluoroscopic images, and these many fluoroscopic images is carried out a spatial filtering handle, and handles image to form many.Be noted that this spatial filtering handle may be selected to be combination that a pixel average treatment, Gauss's smoothing processing and aforementioned two handle one of them.In the present embodiment, handle for carrying out the level and smooth two kinds of calculations of pixel average treatment and Gauss.See also shown in Fig. 6 A to Fig. 6 C, this figure is that spatial filtering of the present invention is handled the image synoptic diagram.With single image is that example is done explanation; Fig. 6 A is a fluoroscopic image 93; Fig. 6 B handles calculation for the image 93a of this fluoroscopic image through gained after the pixel average treatment, the pixel average treatment of present embodiment for 3x3 the brightness value that pixel had averaged.And Fig. 6 C is for pass through the formed image of Fig. 6 B Gauss's smoothing processing image 93b of gained afterwards again.The mode that is noted that spatial filtering is not exceeded with pixel average treatment and Gauss's smoothing processing, and the present invention just is used as the embodiment explanation of Flame Image Process with this two mode.
Shown in Fig. 7 A and Fig. 7 B, this figure is these many formed after treatment burst synoptic diagram of handling in the image in a specific region.Be that many process spatial filterings are handled the formed processing image 93b in back shown in Fig. 7 A.This control module more to these many handle images carry out a calculation handle with this each open the image-region 931 handling to correspond to each specific region 250 on the tissue substance 25 on image 93b had brightness value and converted to current potential; A plurality of to produce respectively about a burst of the specific region 250 on this tissue substance 25, shown in Fig. 7 B.The mode that this calculation is handled belongs to existing technology, does not give unnecessary details at this.Shown in Fig. 7 B, the burst synoptic diagram of one of them specific region 250 in the tissue substance 25 shown in its representative graph 7A.Each burst 94 has a plurality of reorganization electric potential signal 95 serial connections and forms, and each reorganization electric potential signal is made up of 940 of a plurality of signals, and each signal 940 is corresponding with a brightness value of handling the specific region 931 that is had on the image 93b wherein respectively.Be noted that as far as each reorganization electric potential signal, these a plurality of signals 940 are corresponding to the s among Fig. 4 B
0～s
7
But in Fig. 7 B, only pipe has passed through after the spatial filtering, still has some noise, and therefore, this control module is carried out a time Filtering Processing to obtain processing signals sequence 96 as shown in Figure 8 to each burst respectively.Be noted that employed this time filtering processing is that meagre profit comes each burst is carried out filtering with Butterworth LPF (butterworth low-pass filter) in the present embodiment, but do not exceed with Butterworth LPF.Because if the cell in the tissue substance has when unusual; During the action potential that this second pulse signal excites this tissue substance to produce the continuous action electric potential signal changes; Can produce cell membrane potential in the tissue substance changes; The chemical resonant structure of this stain is changed, make stain produce fluorescence and produce transformation.And this transformation promptly can be reflected on the corresponding processing burst.Because each processing signals sequence is each specific region of corresponding tissue substance respectively; Therefore have unusual the time when any one processing signals sequence of discovery, can find out according to this pairing position of abnormality processing burst has unusual zone on the tissue substance.The system of organizing of aforesaid embodiment produces vibration for having suppressant or pressing assembly to avoid tissue substance.In a further embodiment, not containing the inhibitor or for the use of components to inhibit tissue material to suppress vibration of the case, the captured image can be used as a way Martin et al proposed in 2009 and the light for the dynamic image objects Research (Characteristics? of? motion? artifacts? in? cardiac? optical? mapping? studies, 31? Annual? International? Conference? of? IEEE? EMBS? Minneapolis, Minnesota, USA, September? 2-6,2009) or Inagaki (Inagaki), who proposed in 2004 are inhibited in the absence of the state, the heart beat of freedom of high-resolution optical image (High? resolution? optical? mapping? of? cardiac? action? potentials? in? freely? beating? rabbit? hearts, Proceeding? of? the? 26
th ? Annual? International? Conference? of? the? IEEE? EMBS? San? Francisco, CA, USA, September ? 1-5,2004), are not inhibitors or suppress Components, used to avoid an object in the image capturing because of vibration and take to the blurred image.As for handling procedures such as subsequent image processing and signal reorganization, be as previously mentioned, do not do at this and give unnecessary details.
The above is merely embodiments of the invention, when can not with the restriction scope of the invention.The equalization of promptly making according to claim of the present invention generally changes and revises, and will not lose main idea of the present invention place, does not also break away from the spirit and scope of the present invention, and the former capital should be regarded as further enforcement situation of the present invention.
Claims (23)
1. a stroboscopic formula optical imagery image system is characterized in that, includes:
One control module, it postpones control to form a delayed pulse signal to having one-period and being undertaken one by one first pulse signal that a plurality of pulse constituted, and the time interval of the adjacent pulse that this delayed pulse signal had and this cycle have a mistiming;
One light source module; It provides an incident light to shine on a tissue substance that contains a stain; This tissue substance produces a continuous action electric potential signal by one second pulse signal, and this incident light excites this stain in this tissue substance to produce a fluorescence that should continuous action electric potential signal intensity; And
One capturing images unit, it couples with this control module mutually, and this capturing images unit forms many fluoroscopic images according to this this fluorescence of delayed pulse signal acquisition.
2. stroboscopic formula optical imagery image system as claimed in claim 1 is characterized in that, the inhibition means that also include produce vibration to suppress this tissue substance, these inhibition means be a suppressant and a pressing assembly one of them.
3. stroboscopic formula optical imagery image system as claimed in claim 1 is characterized in that, this second pulse signal provides for a physiology second pulse signal that this tissue substance produced or by a signal generation device.
4. stroboscopic formula optical imagery image system as claimed in claim 1 is characterized in that this control module also includes:
One controller, it provides this first pulse signal; And
One delay cell, it couples with this controller and this capturing images unit mutually, and this delay cell is controlled to adjust the triggered time of this first pulse signal by this delay.
5. stroboscopic formula optical imagery image system as claimed in claim 4 is characterized in that this delay cell more couples with this light source module mutually, and this light source module receives this delayed pulse signal, and produces incident light that should delayed pulse signal.
6. stroboscopic formula optical imagery image system as claimed in claim 1 is characterized in that this control module more receives this many fluoroscopic images, and these many fluoroscopic images is carried out a spatial filtering handle, and handles image to form many.
7. stroboscopic formula optical imagery image system as claimed in claim 6 is characterized in that, this spatial filtering be treated to combination that a pixel average treatment, Gauss's smoothing processing and aforementioned two handle wherein it
8. stroboscopic formula optical imagery image system as claimed in claim 6; It is characterized in that; This control module is more handled images to these many and is carried out a calculation and handle to produce a plurality of respectively about a burst of the specific region on this tissue substance; Wherein each burst has a plurality of reorganization electric potential signals serial connection and forms, and each reorganization electric potential signal is made up of a plurality of signal, and each signal is corresponding with a processing image wherein respectively.
9. stroboscopic formula optical imagery image system as claimed in claim 8 is characterized in that, this control module is carried out a time Filtering Processing to obtain a processing signals sequence to each burst respectively.
10. stroboscopic formula optical imagery image system as claimed in claim 9 is characterized in that, this time filtering is treated to uses a Butterworth LPF to come each burst is carried out filtering.
11. stroboscopic formula optical imagery image system as claimed in claim 8 is characterized in that, the mistiming of adjacent reorganization electric potential signal is the time span of at least one action potential ripple of being had in this continuous action electric potential signal.
12. stroboscopic formula optical imagery image system as claimed in claim 1; It is characterized in that; This continuous action electric potential signal is made up of a plurality of action potential ripples, at least one pulse to there being this delayed pulse signal to have in the time range of each action potential ripple.
13. a stroboscopic formula optical imagery image system is characterized in that, includes:
One control module, it postpones control to form a delayed pulse signal to having one-period and being undertaken one by one first pulse signal that a plurality of pulse constituted, and the time interval of the adjacent pulse that this delayed pulse signal had and this cycle have a mistiming;
One light source module; It couples with this control module mutually; To receive this delayed pulse signal; This light source module of the trigger action of this delayed pulse signal produces an incident light and shines on a tissue substance that contains a stain, and this tissue substance produces a continuous action electric potential signal by one second pulse signal, and this incident light excites this stain in this tissue substance to produce a fluorescence that should continuous action electric potential signal intensity; And
One capturing images unit, it captures this fluorescence and forms many fluoroscopic images.
14. stroboscopic formula optical imagery image system as claimed in claim 13 is characterized in that, the inhibition means that also include produce vibration to suppress this tissue substance, these inhibition means be a suppressant and a pressing assembly one of them.
15. stroboscopic formula optical imagery image system as claimed in claim 13 is characterized in that, this second pulse signal provides for a physiology second pulse signal that this tissue substance produced or by a signal generation device.
16. stroboscopic formula optical imagery image system as claimed in claim 13 is characterized in that this control module also includes:
One controller provides this first pulse signal; And
One delay cell couples with this controller and this light source module mutually, and this delay cell is controlled to adjust the triggered time of this first pulse signal by this delay.
17. stroboscopic formula optical imagery image system as claimed in claim 13 is characterized in that this control module also receives this many fluoroscopic images, and these many fluoroscopic images is carried out a spatial filtering handle, and handles image to form many.
18. stroboscopic formula optical imagery image system as claimed in claim 17 is characterized in that, this spatial filtering be treated to combination that a pixel average treatment, Gauss's smoothing processing and aforementioned two handle one of them.
19. stroboscopic formula optical imagery image system as claimed in claim 6; It is characterized in that; This control module is also handled images to these many and is carried out a calculation and handle to produce a plurality of respectively about a burst of the specific region on this tissue substance; Wherein each burst has a plurality of reorganization electric potential signals serial connection and forms, and each reorganization electric potential signal is made up of a plurality of signal, and each signal is corresponding with a processing image wherein respectively.
20. stroboscopic formula optical imagery image system as claimed in claim 19 is characterized in that, this control module is carried out a time Filtering Processing to obtain a processing signals sequence to each burst respectively.
21. stroboscopic formula optical imagery image system as claimed in claim 20 is characterized in that, this time filtering is treated to uses a Butterworth LPF to come each burst is carried out filtering.
22. stroboscopic formula optical imagery image system as claimed in claim 19 is characterized in that, the mistiming of adjacent reorganization electric potential signal is the time span of at least one action potential ripple of being had in this continuous action electric potential signal.
23. stroboscopic formula optical imagery image system as claimed in claim 13; It is characterized in that; This continuous action electric potential signal is made up of a plurality of action potential ripples, at least one pulse to there being this delayed pulse signal to have in the time range of each action potential ripple.
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| Publication number | Priority date | Publication date | Assignee | Title |
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2011
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| JPH04270940A (en) * | 1991-02-27 | 1992-09-28 | Toa Medical Electronics Co Ltd | Flow image sight meter |
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| CN102809551B (en) | 2015-06-24 |
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