CN102809551B - Stroboscopic optical image mapping system - Google Patents
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- CN102809551B CN102809551B CN201110145593.7A CN201110145593A CN102809551B CN 102809551 B CN102809551 B CN 102809551B CN 201110145593 A CN201110145593 A CN 201110145593A CN 102809551 B CN102809551 B CN 102809551B
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Abstract
The invention provides a stroboscopic optical image mapping system which comprises a control module, a light source module and an image acquisition unit. The control module actuates a delay control to a first pulse signal which has a cycle and consists of a plurality of pulses, so that a delayed pulse signal is formed. The time interval of adjacent pulses of the delayed pulse signal and the cycle are provided with a time difference. The light source provides an incident beam irradiating an organization containing dye. The organization generates a continuous action level signal by the aid of a second pulse signal. The incident beam triggers the dye in the organization to generate a fluorescent beam as intense as the continuous action level signal. The image acquisition unit acquires the fluorescent beam to generate a plurality of fluorescent images according to the delayed pulse signal.
Description
Technical field
The present invention is a kind of optical system, refers in particular to a kind of strobe type optical imagery image system utilizing stroboscopic technique.
Background technology
Cell (the excitable cells of excitatory potential can be produced in biosome; As neurocyte or cardiac muscle cell etc..), producing the change of intraor extracellular potential difference (PD) via the penetrating of ion on cell membrane, is maintain the suitable critical function of these cell normal physiologicals.The cell that can produce excitatory potential bears the transmission (cell-cell communication) of cell-tocell and the initiation (triggering action potential) of excitatory potential.By the cell phase composition of excitatory potential organizing telecommunication, research cell membrane potential change or organize action potential (action potentials) to change and affect the science of the physiological change of biosome, is called electro physiology; If be limited in heart cell or organize, what claim is cardiac electrophysiology.Cardiac electrophysiology is research cardiac function and the main pointer of pathology, and one of optical imagery image system instrument that to be research cardiac electrophysiology main, but legacy optical imagery image system its use and still have shortcoming to solve.Legacy optical imagery image system is mainly containing biosome heart tissue, optical instrument and detection record device.Native system utilizes the stain (Voltage-sensitive dye) of cell membrane potential sensitive, produces cell membrane potential change during action potential changes, and the chemical resonant structure of stain is changed, and thus the fluorescence excitation of stain produces transformation.Fluorescence signal through the amplification of optical instrument, by capturing images unit (such as: CCD) detection record.Existing instrument has its property do not replaced for cardiac electrophysiology stud, and its advantage comprises: (1) utilizes optical scanning, with action potential telecommunication change in not direct contact tissue mode collection organization.(2) carry out organizing telecommunication to detect than array electrode approach higher pixel.(3) not by the telecommunication of extraneous noise undesired signal.But it must have expensive quickly picking image camera and jumbo image file storage hardware, existing instrument cannot generally be used because of expensive and time and the restriction of shadow matter resolution, so this patent will improve for these shortcomings, thus develop the strobe type optical imagery image system of a new generation.
As shown in Figure 1, this figure is existing optical imagery image system schematic diagram.This optical imagery image system 1 is mainly containing tissue substance 10, optical instrument 11 and capturing images unit 12.This tissue substance 10 is arranged on plummer 13, and this optical instrument 11 is made up of light source 110, collimating mirror group 111, spectroscope 112, filter 113 and lens combination 114.The system of Fig. 1 utilizes the stain (Voltage-sensitive dye) of cell membrane potential sensitive, produces cell membrane potential change during action potential changes, and the chemical resonant structure of stain is changed, and thus the fluorescence excitation of stain produces transformation.Fluorescence signal through the amplification of optical instrument 11, by capturing images unit 12 detection record.Although existing optical imagery image system 1 provides one and need not contact animal hearts and can observe the method that heart electrically changes.But this optical imagery image system 1 needs data acquisition speed fast on hardware, therefore high on cost.
In addition, in the prior art as US publication US.Pub.No.2008/0188727 discloses a kind of solid broadband spectral device, its broadband light utilizing light emitting diode to produce, on tissue substance, can provide illumination due to light-source structure design nor can increase the temperature of tissue substance.Therefore this light source can be incorporated into medical detecting device or other is based on the detection system such as optical scattering or fluorescence.In addition, US Patent No. .Pat.No.6,680,780 a kind of laser interference systems detecting movable object of instruction.In the art, probe is arranged on a control device, and this probe can produce corresponding movement according to voltage, and then laser is divided into reference light and exciting light, the object light formed when exciting light is projected on object and reference light are interfered and form interference fringe.Then determine displacement and the sense of displacement of object according to interference fringe, then convert displacement to voltage, and then make this probe produce displacement according to this voltage.
Summary of the invention
The invention provides a kind of strobe type optical imagery image system, it utilizes the mechanism of stroboscopic and delay, capture respectively about a tissue substance under an excited state, the fluoroscopic image that corresponding different time delay puts, by image procossing and filtering calculation, the fluoroscopic image of multiple corresponding difference points time delays by this is changed and reassembles into, the processing signals sequence under this excited state can be represented, to judge the whether normal foundation of this tissue substance as follow-up.
The invention provides a kind of strobe type optical imagery image system, it utilizes the mechanism of stroboscopic and delay, can expensive capturing images unit need not be used namely can to destroy fast, or not contact tissue mode, tissues observed electro physiology changes, importantly this technology provides sensitive and high-resolution image quality, high-resolution fluoroscopic image can be obtained, in order to follow-up calculation and signal transacting.
In one embodiment, the invention provides a kind of strobe type optical imagery image system, it includes: a control module, it carries out a Time delay control to form a delayed pulse signal to one first pulse signal having a cycle and be made up of multiple pulse, and the time interval and this cycle of the adjacent pulse that this delayed pulse signal has have a mistiming; One light source module, it provides an incident light to irradiate on the tissue substance containing a stain, this tissue substance produces a continuous action electric potential signal by one second pulse signal, and this incident light excites this stain in this tissue substance to produce should a fluorescence of continuous action electric potential signal intensity; And a capturing images unit, it couples mutually with this control module, and this capturing images unit captures this fluorescence according to this delayed pulse signal and forms multiple fluoroscopic images.
In another embodiment, the invention provides a kind of strobe type optical imagery image system, it includes: a control module, it carries out a Time delay control to form a delayed pulse signal to one first pulse signal having a cycle and be made up of multiple pulse, and the time interval and this cycle of the adjacent pulse that this delayed pulse signal has have a mistiming; One light source module, it couples mutually with this control module, to receive this delayed pulse signal, this light source module of the trigger action of this delayed pulse signal produces an incident light and irradiates on the tissue substance containing a stain, this tissue substance produces a continuous action electric potential signal by one second pulse signal, and this incident light excites this stain in this tissue substance to produce should a fluorescence of continuous action electric potential signal intensity; And a capturing images unit, it captures this fluorescence and forms multiple fluoroscopic images.
Accompanying drawing explanation
Fig. 1 is existing optical imagery image system schematic diagram;
Fig. 2 A is strobe type optical imagery image system first embodiment configuration diagram of the present invention;
Fig. 2 B is strobe type optical imagery image system second embodiment configuration diagram of the present invention;
Fig. 2 C is strobe type optical imagery image system the 3rd embodiment configuration diagram of the present invention;
Fig. 2 D is that the present invention utilizes pressing assembly to suppress schematic diagram on tissue substance;
Fig. 3 A is the first pulse signal schematic diagram;
Fig. 3 B is depicted as delayed pulse signal schematic diagram;
Fig. 4 A is that delayed pulse signal and tissue substance are stimulated the continuous action electric potential signal relation schematic diagram produced;
Fig. 4 B is electric potential signal of the present invention restructuring schematic diagram;
Fig. 4 C is that delayed pulse signal of the present invention and continuous action electric potential signal are related to another embodiment schematic diagram;
Fig. 5 A and Fig. 5 B is these multiple fluoroscopic images and the original second pulse signal schematic diagram about the specific region on this tissue substance;
Fig. 5 C and Fig. 5 D is respectively signal of the present invention and recombinates embodiment schematic diagram;
Fig. 6 A to Fig. 6 C is spatial filtering process image schematic diagram of the present invention;
Fig. 7 A and Fig. 7 B processes a specific region in image burst schematic diagram formed after treatment for these multiple;
Fig. 8 is processing signals sequence diagram.
Wherein, Reference numeral:
1-optical imagery image system
10-tissue substance
11-optical instrument
110-light source
111-collimating mirror group
112-spectroscope
113-filter
114-lens combination
12-capturing images unit
13-plummer
2-strobe type optical imagery image system
20-control module
200-controller
201-first delay cell
202-second delay cell
21-signal generation device
22-plummer
23-light source module
24-capturing images unit
25-tissue substance
250-specific region
26-color separation spectroscope
27,28-wave length filtering sheet
29-pressing assembly
90-first pulse signal
900-pulse
91-delayed pulse signal
910-pulse
97-continuous action electric potential signal
970-action potential ripple
92-recombinates electric potential signal
93,93a, 93b-image
930-position
931-image-region
94-burst
940-signal
95-recombinates electric potential signal
96-processing signals sequence
Embodiment
Further cognitive and understanding is had to feature of the present invention, object and function for making auditor, hereafter the relevant thin portion structure of device of the present invention and the theory reason of design are described by spy, to make auditor can understand feature of the present invention, detailed description is presented below:
Refer to shown in Fig. 2 A, this figure is strobe type optical imagery image system first embodiment configuration diagram of the present invention.In the present embodiment, this strobe type optical imagery image system 2 includes control module 20, signal generation device 21, plummer 22, light source module 23 and a capturing images unit 24.This control module 20, it carries out a Time delay control to form a delayed pulse signal to one first pulse signal.Refer to shown in Fig. 3 A, this figure is the first pulse signal schematic diagram.This first pulse signal 90 has a cycle T and is made up of multiple pulse 900.Refer to shown in Fig. 3 B, this figure is delayed pulse signal schematic diagram.In order to reach the effect of stroboscopic acquire images, this control module 20 carries out Time delay control to adjust the triggered time of each pulse of this first pulse signal to this first pulse signal 90, and the time interval Δ T of the adjacent pulse 910 that the delayed pulse signal 91 produced after making Time delay control has and this cycle T have a mistiming Δ t.
Can find out in figure 3b at the 0th time point t
0time, after triggering the pulse formed, make at the 1st time point t via Time delay control
1the pulse triggered and the 0th time point t
0pulse between time interval Δ T and this cycle T between there is a mistiming Δ t.When to the n-th time point, by the 1 to the n-th time point mistiming of adding up equal this cycle length of T time, then get back to again as t when the time point of (n+1)th
0the trigger pulse state of time point, periodically analogizes with this.
Return shown in Fig. 2 A, in the present embodiment, this control module 20 includes controller 200 and one first delay cell 201.This controller 200 provides this first pulse signal.This controller 200 can for be integrated with signal produce function have calculation process can computing machine, workstation or server, but not as limit.Such as: the mode that the computing machine of operation processing function and signal generator are provided separately.This first delay cell 201, it couples mutually with this controller 200 and this capturing images unit 24, this first delay cell 201 carries out this Time delay control to this first pulse signal, makes interpulse the had time span of adjacent different time points and recurrence interval have the mistiming.Although be noted that this first delay cell 201 is separated with this controller 200 in the embodiment of Fig. 2 A, in another embodiment, this first delay cell 201 can also be combined to form single control module with this controller 200.This is that those skilled in the art can spirit according to the present invention be changed.
In the present embodiment, this signal generation device 21 produces one second pulse signal.This plummer 22, it provides carrying one tissue substance 25.This tissue substance 25 can be biological tissue, such as: nerve fiber, musculature or heart tissue.In the present embodiment, this tissue substance 25 is heart tissue.Containing an inhibitor and a stain in this tissue substance 25, this tissue substance 25 and this signal generation device 21 couple to receive this second pulse signal mutually.When this tissue substance 25 receives this second pulse signal, a continuous action electric potential signal can be produced.This stain belongs to the stain (Voltage-sensitive dye) by the cell membrane potential sensitivity to heart cell, and this stain can be chosen as (di-4-ANEPPs), but not as limit.Due to this tissue substance 25 receive this second pulse signal time, vibration can be produced because of the pulse of the second pulse signal, therefore this inhibitor its in order to suppress this tissue substance 25 to receive this second pulse signal time the vibration that produces.In the present embodiment, this inhibitor is Cytochalasin D (Cyto D), but not as limit.Be noted that and suppress this tissue substance 25 because receive the means of the vibration that this second pulse signal produces, except aforementioned to except tissue substance 25 injecting inhibitor, pressing assembly 29 can also be used, as shown in Figure 2 D.In the present embodiment, this pressing assembly 29 is suppressed for slide and is vibrated to avoid tissue substance to produce on tissue substance.
This light source module 23, it provides the incident light matched with this stain to irradiate on this tissue substance 25, and this incident light excites this stain in this tissue substance to produce should a fluorescence of continuous action electric potential signal intensity.In the present embodiment, this light source module 23 is a light emitting diode (light emitting diode, LED) light source module.Because this incident light need mate with this stain, stain just can be excited to produce fluorescence, and therefore in the present embodiment, this incident spectrum is its top efficiency wavelength with 475nm, and fluorescence excitation wavelength is 617nm.Be noted that the selection of the most high-efficient filter wavelength of this incident light is determined according to stain kind, therefore not with aforesaid embodiment for restriction.
This capturing images unit 24, it couples mutually with this control module 20, multiple fluoroscopic images that this capturing images unit 24 captures this fluorescence according to this delayed pulse signal and formed about this tissue substance 25.Refer to shown in Fig. 3 B, in figure 3b, during each pulses generation in delayed pulse signal, this capturing images unit 24 i.e. fechtable fluoroscopic image.This capturing images unit 24 can be chosen as Charged Coupled Device (Charge-coupled Device, the capturing images unit of capturing images unit CCD) or complementary metal-oxide layer-semiconductor (Complementary Metal-Oxide-Semiconductor, CMOS).The present embodiment selects CCD to be capturing images unit.In addition, be noted that and have more a color separation spectroscope 26 and wave length filtering sheet 27 and 28 between this capturing images unit 24 and this tissue substance 25, to guarantee that wavelength efficiency is for the best, and the stray light avoiding CCD to be subject to beyond fluorescence excitation.
Except stretching frame structure shown in Fig. 2 A, as shown in Figure 2 B, this figure is strobe type optical imagery image system second embodiment configuration diagram of the present invention.In the present embodiment, be with the difference of Fig. 2 A, this control module 20 more provides this delayed pulse signal to this light source module 23.This light source module 23 is made to produce the frequency of incident light and the Frequency Synchronization of this capturing images unit 24 acquire images.Refer to shown in Fig. 2 C, this figure is strobe type optical imagery image system the 3rd embodiment configuration diagram of the present invention.Framework in the present embodiment, substantially with Fig. 2 category-A seemingly, difference be that delayed pulse signal that the control module 20 of the present embodiment produces only is supplied to this light source module 23 and carries out Time delay control.
Utilize after the system of Fig. 2 A to Fig. 2 C captures multiple fluoroscopic images, this control module 20 carries out image and signal transacting to these multiple fluoroscopic images.First the principle of image of the present invention and signal transacting is described, refers to shown in Fig. 4 A, this figure is that delayed pulse signal and tissue substance are stimulated the continuous action electric potential signal relation schematic diagram produced.In order to save system cost, the capturing images unit used in the embodiment of the present invention is the capturing images unit of low speed.The potential change that fluorescence has after conversion is obtained in order to obtain being stimulated about this tissue substance.The present invention utilizes the pulse signal of out of phase to capture the fluorescence information of diverse location, and the mode of recycling computing with words forms one group of new data, utilizes this group new data to judge whether tissue substance has exception.In Figure 4 A, label 97 represents tissue substance when receiving the second pulse signal, the continuous action electric potential signal produced, and this continuous action electric potential signal is made up of multiple action potential ripple 970; And label 91 represents the delayed pulse signal through delay disposal, it is made up of multiple pulse 910.
In the present embodiment, each action potential ripple 970 is to there being a pulse 910.When each pulse 910 produces, namely capturing images unit can produce a fluoroscopic image.Although each pulse drive capturing images unit the picture position about this continuous action electric potential signal 97 that captures different, the electric potential signal s corresponding to image that different time points can be captured after being through back segment process
0~ s
7restructuring, to form new restructuring electric potential signal 92, as shown in Figure 4 B.That is, in the present embodiment, the information s of the restructuring electric potential signal 92 shown in Fig. 4 B for utilizing the pulse 910 of 8 delayed pulse signals 91 to capture this continuous action electric potential signal 97 gained
0~ s
7combine.To so repeatedly carrying out, also can produce about one group of this signal new restructuring electric potential signal.Be noted that Fig. 4 A in addition and be the corresponding pulse of each action potential ripple shown by the embodiment in Fig. 4 B.In another embodiment, as shown in Figure 4 C, each action potential ripple 970 multiple pulses of correspondence.The general practice can use and form the state as Fig. 4 C than pulse signal faster, the quantity of the action potential ripple of sampling composition one restructuring required for electric potential signal will be utilized thus to reduce, that is can increase the efficiency of restructuring.
The mode that following explanation control module performs, refers to shown in Fig. 5 A and Fig. 5 B, and this figure is these multiple fluoroscopic images and electric potential signal schematic diagram of recombinating about one of the specific region on this tissue substance.Be multiple fluoroscopic images 93 that this capturing images unit captures in fig. 5.Each pixel in each fluoroscopic image is ad-hoc location different on this tissue substance of representative.And in Fig. 5 B, then for should the electrical signal sequence schematic diagram that obtains after conversion of an ad-hoc location has on tissue substance fluoroscopic image.Example is depicted as, on the ad-hoc location 930 of the ad-hoc location on tissue substance in fluoroscopic image with Fig. 5 A.The restructuring electric potential signal that process conversion is formed as shown in Figure 5 B.As shown in Fig. 5 C and Fig. 5 D, this figure is respectively signal of the present invention restructuring embodiment schematic diagram.In the embodiment of Fig. 5 C, restructuring electric potential signal 92 combined for multiple action potential ripple 970 (the present embodiment is four, and the action potential wave number amount of its restructuring institute palpus is determined as required, is not limited with these four).Adjacent restructuring electric potential signal 92 then differs four action potential ripples, 970 elapsed times.And in figure 5d, belong to a kind of signal recombination form of restructuring electric potential signal in real time.That is, when the restructuring electric potential signal 92 of first completes restructuring, as long as again through an action potential ripple 970 elapsed times, namely can again by last three the action potential ripples required for the previous restructuring electric potential signal 92 of restructuring, and reassemble into a new restructuring electric potential signal again, and then reach the effect of restructuring in real time.
Can find out that the pulse potential signal formed after restructuring is subject to very large noise by Fig. 5 B, therefore be not easy to judge.Therefore, this control module of the present invention more receives this multiple fluoroscopic images, and carries out a spatial filtering process to these multiple fluoroscopic images, to form multiple process images.Be noted that this spatial filtering process may be selected to be a pixel average treatment, a Gaussian smoothing and aforementioned two process combinations one of them.In the present embodiment, for carrying out pixel average treatment and Gaussian smoothing two kinds calculation process.Refer to shown in Fig. 6 A to Fig. 6 C, this figure is spatial filtering process image schematic diagram of the present invention.Explain for single image, Fig. 6 A is a fluoroscopic image 93, Fig. 6 B is the image 93a of this fluoroscopic image gained after pixel average treatment, and the pixel average treatment of the present embodiment is averaging processing calculation for brightness value 3x3 pixel had.And Fig. 6 C is the image that formed by Fig. 6 B image 93b of gained after Gaussian smoothing again.Be noted that the mode of spatial filtering is not limited with pixel average treatment and Gaussian smoothing, the present invention is just used as the embodiment explanation of image procossing in this two mode.
As shown in Fig. 7 A and Fig. 7 B, this figure is formed the after treatment burst schematic diagram in the specific region in these multiple process images.Be depicted as the process image 93b that multiple are formed after spatial filtering process in fig. 7.This control module more to this multiple process images carry out a calculation process with each process image 93b by this corresponds to the image-region 931 of each specific region 250 on tissue substance 25 there is brightness value convert current potential to, multiple respectively about a burst of the specific region 250 on this tissue substance 25 to produce, as shown in Figure 7 B.The mode of this calculation process belongs to existing technology, and therefore not to repeat here.As shown in Figure 7 B, the burst schematic diagram of one of them specific region 250 in tissue substance 25 shown in its representative graph 7A.Each burst 94 has multiple restructuring electric potential signal 95 serial connection and forms, and each restructuring electric potential signal is made up of multiple signal 940, and each signal 940 is corresponding with the brightness value that wherein processes the specific region 931 that image 93b has respectively.Be noted that, for each restructuring electric potential signal, the plurality of signal 940 is corresponding to the s in Fig. 4 B
0~ s
7.
But in figure 7b, after only pipe have passed through spatial filtering, still have some noise, therefore, this control module carries out a time filtering process to obtain processing signals sequence 96 as shown in Figure 8 to each burst respectively.Be noted that this time filtering process system meagre profit Butterworth LPF (butterworth low-pass filter) used in the present embodiment carries out filtering to each burst, but be not limited with Butterworth LPF.If due to the cell in tissue substance have abnormal time, this second pulse signal excite this tissue substance produce continuous action electric potential signal action potential change during, cell membrane potential change can be produced in tissue substance, the chemical resonant structure of this stain is changed, makes stain produce fluorescence and produce transformation.And namely this transformation can react in the processing signals sequence of correspondence.Due to each specific region of the corresponding tissue substance of each processing signals sequence difference, therefore, in time finding that any one processing signals sequence has abnormal, can find out on tissue substance and have abnormal region in the position corresponding to this abnormality treatment signal sequence.The tissue substance of aforesaid embodiment is have inhibitor or pressing assembly to produce vibration to avoid tissue substance.In a further embodiment, for not containing inhibitor or utilizing pressing assembly to the situation suppressing tissue substance to vibrate, the mode of its acquire images can utilize as the people such as Martin 2009 research (Characteristics of motion artifacts in cardiac optical mapping studies dynamic object being carried out to light reflection that proposes, 31 Annual International Conference of IEEE EMBS Minneapolis, Minnesota, USA, September 2-6, 2009) people such as Huo person Shi Rice wall (Inagaki) proposed in 2004 under the state be not suppressed, the height that heart is freely beated resolves optical mapping research (High resolution optical mapping of cardiac action potentials in freely beating rabbit hearts, Proceeding of the 26
thannual International Conference of the IEEE EMBS San Francisco, CA, USA, September 1-5,2004), be all without under inhibitor or pressing assembly, be used for avoiding object when capturing images because of vibration and get fuzzy image.As for handling procedures such as follow-up image procossing and signal restructuring, be as previously mentioned, do not repeat at this.
The above, be only embodiments of the invention, when can not with the restriction scope of the invention.Namely the equalization generally made according to the present patent application the scope of the claims changes and amendment, and will not lose main idea place of the present invention, also not depart from the spirit and scope of the present invention, former capital should be considered as further status of implementation of the present invention.
Claims (19)
1. a strobe type optical imagery image system, is characterized in that, includes:
One control module, it carries out a Time delay control to form a delayed pulse signal to one first pulse signal having a cycle and be made up of multiple pulse, and the time interval and this cycle of the adjacent pulse that this delayed pulse signal has have a mistiming;
One light source module, it provides an incident light to irradiate on the tissue substance containing a stain, this tissue substance produces a continuous action electric potential signal by one second pulse signal, this incident light excites this stain in this tissue substance to produce should a fluorescence of continuous action electric potential signal intensity, and this incident light and this stain match; And
One capturing images unit, it couples mutually with this control module, and this capturing images unit captures this fluorescence according to this delayed pulse signal and forms multiple fluoroscopic images;
Wherein, this control module receives this multiple fluoroscopic images, and a spatial filtering process is carried out to these multiple fluoroscopic images, to form multiple process images, this control module to this multiple process images to carry out a calculation process multiple respectively about a burst of the specific region on this tissue substance to produce, wherein each burst have multiple restructuring electric potential signal serial connection form, and each restructuring electric potential signal is made up of multiple signal, it is corresponding that each signal processes image with wherein respectively.
2. strobe type optical imagery image system as claimed in claim 1, is characterized in that, the suppression means that also include produce vibration to suppress this tissue substance, these suppression means be an inhibitor and a pressing assembly one of them.
3. strobe type optical imagery image system as claimed in claim 1, is characterized in that, physiology second pulse signal that this second pulse signal produces for this tissue substance or provide by a signal generation device.
4. strobe type optical imagery image system as claimed in claim 1, it is characterized in that, this control module also includes:
One controller, it provides this first pulse signal; And
One delay cell, it couples mutually with this controller and this capturing images unit, this delay cell by this Time delay control to adjust the triggered time of this first pulse signal.
5. strobe type optical imagery image system as claimed in claim 4, it is characterized in that, this delay cell more couples mutually with this light source module, and this light source module receives this delayed pulse signal, and produces should the incident light of delayed pulse signal.
6. strobe type optical imagery image system as claimed in claim 1, is characterized in that, this spatial filtering is treated to one of them of combinations of a pixel average treatment, a Gaussian smoothing and aforementioned two process.
7. strobe type optical imagery image system as claimed in claim 1, it is characterized in that, this control module carries out a time filtering process to obtain a processing signals sequence to each burst respectively.
8. strobe type optical imagery image system as claimed in claim 7, it is characterized in that, this time filtering is treated to use one Butterworth LPF and carries out filtering to each burst.
9. strobe type optical imagery image system as claimed in claim 1, is characterized in that, mistiming of adjacent restructuring electric potential signal time span of at least one action potential ripple for having in this continuous action electric potential signal.
10. strobe type optical imagery image system as claimed in claim 1, it is characterized in that, this continuous action electric potential signal is made up of multiple action potential ripple, at least one pulse that this delayed pulse signal should be had to have in the time range of each action potential ripple.
11. 1 kinds of strobe type optical imagery image systems, is characterized in that, include:
One control module, it carries out a Time delay control to form a delayed pulse signal to one first pulse signal having a cycle and be made up of multiple pulse, and the time interval and this cycle of the adjacent pulse that this delayed pulse signal has have a mistiming;
One light source module, it couples mutually with this control module, to receive this delayed pulse signal, this light source module of the trigger action of this delayed pulse signal produces an incident light and irradiates on the tissue substance containing a stain, this tissue substance produces a continuous action electric potential signal by one second pulse signal, this incident light excites this stain in this tissue substance to produce should a fluorescence of continuous action electric potential signal intensity, and this incident light and this stain match; And
One capturing images unit, it captures this fluorescence and forms multiple fluoroscopic images;
Wherein, this control module also receives this multiple fluoroscopic images, and a spatial filtering process is carried out to these multiple fluoroscopic images, to form multiple process images, this control module also to this multiple process images to carry out a calculation process multiple respectively about a burst of the specific region on this tissue substance to produce, wherein each burst have multiple restructuring electric potential signal serial connection form, and each restructuring electric potential signal is made up of multiple signal, it is corresponding that each signal processes image with wherein respectively.
12. strobe type optical imagery image systems as claimed in claim 11, is characterized in that, the suppression means that also include produce vibration to suppress this tissue substance, these suppression means be an inhibitor and a pressing assembly one of them.
13. strobe type optical imagery image systems as claimed in claim 11, is characterized in that, physiology second pulse signal that this second pulse signal produces for this tissue substance or provide by a signal generation device.
14. strobe type optical imagery image systems as claimed in claim 11, it is characterized in that, this control module also includes:
One controller, provides this first pulse signal; And
One delay cell, couples mutually with this controller and this light source module, this delay cell by this Time delay control to adjust the triggered time of this first pulse signal.
15. strobe type optical imagery image systems as claimed in claim 11, is characterized in that, this spatial filtering be treated to a pixel average treatment, a Gaussian smoothing and aforementioned two process combinations one of them.
16. strobe type optical imagery image systems as claimed in claim 11, is characterized in that, this control module carries out a time filtering process to obtain a processing signals sequence to each burst respectively.
17. strobe type optical imagery image systems as claimed in claim 16, is characterized in that, this time filtering is treated to use one Butterworth LPF and carries out filtering to each burst.
18. strobe type optical imagery image systems as claimed in claim 11, is characterized in that, mistiming of adjacent restructuring electric potential signal time span of at least one action potential ripple for having in this continuous action electric potential signal.
19. strobe type optical imagery image systems as claimed in claim 11, it is characterized in that, this continuous action electric potential signal is made up of multiple action potential ripple, at least one pulse that this delayed pulse signal should be had to have in the time range of each action potential ripple.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6680780B1 (en) * | 1999-12-23 | 2004-01-20 | Agere Systems, Inc. | Interferometric probe stabilization relative to subject movement |
| CN1661361A (en) * | 2005-01-31 | 2005-08-31 | 浙江大学 | Detection method suited on surface of spherical fruit triggered to collect images based on need and equipment |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3084296B2 (en) * | 1991-02-27 | 2000-09-04 | シスメックス株式会社 | Flow image cytometer |
| JP2005049282A (en) * | 2003-07-30 | 2005-02-24 | Hamamatsu Photonics Kk | Fluorescent observation apparatus and laser light irradiation device |
-
2011
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6680780B1 (en) * | 1999-12-23 | 2004-01-20 | Agere Systems, Inc. | Interferometric probe stabilization relative to subject movement |
| CN1661361A (en) * | 2005-01-31 | 2005-08-31 | 浙江大学 | Detection method suited on surface of spherical fruit triggered to collect images based on need and equipment |
Non-Patent Citations (5)
| Title |
|---|
| "Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging";Nishigaki T 等;《Biotechniques》;20060831;第41卷(第2期);全文 * |
| "基于ANEP染料荧光光谱迁移的单波长心脏光学标测系统";王晶 等;《光谱学与光谱分析》;20080331;第28卷(第3期);全文 * |
| "心肌膜电位的光学标测技术及实验研究";张虹 等;《光子学报》;20080331;第37卷(第3期);全文 * |
| "电压敏感染料膜电位光学标测技术";张虹 等;《生物医学工程学》;20060721;第23卷(第3期);第665-668页 * |
| 卢熙 等."心脏电活动高分辨率光学标测系统的光路设计".《2007中国生物医学工程联合学术年会论文集(下册)》.2007,978-980. * |
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