CN102911997A - Culture medium used for detecting biological phosphorus removal microorganism and method for detecting phosphorous removal capability of biological phosphorus removal microorganism - Google Patents

Culture medium used for detecting biological phosphorus removal microorganism and method for detecting phosphorous removal capability of biological phosphorus removal microorganism Download PDF

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CN102911997A
CN102911997A CN2012104378744A CN201210437874A CN102911997A CN 102911997 A CN102911997 A CN 102911997A CN 2012104378744 A CN2012104378744 A CN 2012104378744A CN 201210437874 A CN201210437874 A CN 201210437874A CN 102911997 A CN102911997 A CN 102911997A
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concentration
substratum
detection
centrifuge tube
microorganism
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CN102911997B (en
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亢涵
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Shenyang Jianzhu University
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Shenyang Jianzhu University
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Abstract

The invention discloses culture mediums used for detecting a biological phosphorus removal microorganism and a method for detecting the phosphorous removal capability of the biological phosphorus removal microorganism, relating to culture mediums used for detecting a phosphorus removal microorganism and a method for detecting the phosphorous removal capability of the phosphorus removal microorganism. The invention solves the problems that the traditional culture medium used for detection has poor pertinence, the used culture medium is not applicable to the detection of the phosphorous removal capability of the biological phosphorus removal microorganism and the phosphorus removal method of the phosphorus removal microorganism is inappropriate. The culture mediums used for detection comprise an anaerobic detection culture medium and an aerobic detection culture medium. The method comprises the following steps of: (1) preparing the culture mediums; (2) taking a pure bacterium liquid of the phosphorus removal microorganism; (3) adding the anaerobic detection culture medium; (4 adding the aerobic detection culture medium; (6) adding the anaerobic detection culture medium; (7) circularly operating the steps (4) to (6) for twice to four times; (8) detecting the phosphorous removal capability; and (9) performing microscopic examination on a pure bacterial strain. The method provided by the invention has strong pertinence, and the culture mediums are applicable to the growing and phosphorous removal capability detection of the phosphorus removal microorganism.

Description

The biological phosphate-eliminating microorganism detection is with the method for substratum and detection of biological dephosphorization microorganism dephosphorization ability
Technical field
The present invention relates to a kind of dephosphorization microorganism detection with the method for substratum and detection of biological dephosphorization microorganism dephosphorization ability.
Background technology
The method of the pure bacterial strain dephosphorization of detection of biological dephosphorization microorganism ability is that the pure bacterial strain that will screen from Biological Phosphorus Removal System places the shaking flask that contains beef-protein medium to cultivate at present, analyze again the phosphate content in the bacterium liquid after cultivating, thereby obtain the dephosphorization ability of pure bacterial strain, but also there is following problem in the method: 1, this method specific aim is poor, it is enrichment and the reproductive process of pure bacterial strain, under this aerobic conditions, no matter carbon source and phosphoric acid salt which kind of microorganism can absorb in the substrate carry out the bacterial strain breeding, so the net result that records and untrue; 2, nutritive ingredient is unreasonable in the nutrient broth medium that adopts of this method, so that the biological phosphate-eliminating microorganism after cultivating has lost the dephosphorization ability under the reactor operational conditions.
Summary of the invention
The objective of the invention is for the method specific aim that solves existing detection of biological dephosphorization microorganism dephosphorization ability poor, the irrational problem of medium nutrient content.And provide the method for a kind of biological phosphate-eliminating microorganism detection with substratum and detection of biological dephosphorization microorganism dephosphorization ability.
Biological phosphate-eliminating microorganism detection substratum of the present invention is two kinds of substratum that biological phosphate-eliminating Institute of Micro-biology detects usefulness successively, according to for detection of two kinds of substratum of order be respectively anaerobism and detect substratum, aerobic detection substratum; Wherein every liter of anaerobism detection substratum is by the NaAc of 0.3 ~ 0.5g, (NH of 270 ~ 290mg 4) 2SO 4, 27 ~ 29mg CaCl 22H 2The MgSO of O, 350 ~ 370mg 47H 2The VITAMIN liquid of the liquid microelement of O, 0.5 ~ 0.7mL, 1.8 ~ 2.4mL and the distilled water of surplus form, and the pH value that anaerobism detects substratum is 6.9 ~ 7.4; Every liter of aerobic detection substratum is the KH by 70 ~ 80mg 2PO 4, 125 ~ 135mg K 2HPO 4, 260 ~ 300mg (NH 4) 2SO 4, 26 ~ 30mg CaCl 22H 2The MgSO of O, 340 ~ 380mg 47H 2The VITAMIN liquid of the liquid microelement of O, 0.5 ~ 1.2mL, 1.5 ~ 3mL and the distilled water of surplus form, and the pH value of aerobic detection substratum is 6.9 ~ 7.4.
Wherein, component and the concentration in the liquid microelement in anaerobism detection substratum, the aerobic detection substratum is as follows: FeCl 36H 2The concentration of O is 2.5 ~ 3.5g/L, H 3BO 3Concentration be 0.25 ~ 0.35g/L, CuSO 45H 2The concentration of O is that the concentration of 0.05 ~ 0.07g/L, KI is 0.32 ~ 0.4g/L, MnCl 24H 2The concentration of O is 0.2 ~ 0.28g/L, Na 2MO 42H 2The concentration of O is 0.1 ~ 0.14g/L, ZnSO 47H 2The concentration of O is 0.2 ~ 0.28g/L and CoCl 26H 2The concentration of O is 0.28 ~ 0.32g/L.
Component and concentration in the VITAMIN liquid are as follows: vitamins B 1Concentration be 80 ~ 120mg/L, vitamins B 2Concentration is 80 ~ 120mg/L, vitamins B 6Concentration be 180 ~ 220mg/L, vitamins B 12Concentration be that the concentration of 1.8 ~ 2.2g/L, folic acid is that the concentration of 36 ~ 44mg/L, nicotinic acid is 80 ~ 120mg/L, the concentration of calcium pantothenate is that the concentration of 80 ~ 120mg/L, para-amino benzoic acid is that the concentration of 80 ~ 120mg/L and vitamin H is 36 ~ 44mg/L.
The method of detection of biological dephosphorization microorganism dephosphorization ability of the present invention is carried out according to following steps: one, preparation anaerobism recited above detects substratum, aerobic detection substratum; Two, get the bacterium liquid that 1mL separates the pure bacterial strain obtain and place the aerobic detection substratum of 50mL step 1 preparation, in 16 ~ 20 ℃ of shaking tables, cultivated 1 ~ 1.5 day, the bacterium liquid that again cultivation is obtained is poured in the centrifuge tube of 50mL, under 5000 ~ 6000 rev/mins condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Three, add the anaerobism detection substratum mixing that the 30ml step 1 is prepared again in the 50mL centrifuge tube, be filled with nitrogen 10 minutes in the 50mL centrifuge tube, sealing places 17 ~ 21 ℃ shaking table to cultivate 1.5 ~ 2.5 hours; Four, centrifuge tube is placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Five, add the aerobic detection substratum mixing that the 30ml step 1 is prepared again in the centrifuge tube of step 4 50mL, aeration is 1.5 ~ 2.5 hours at ambient temperature; Six, centrifuge tube is placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Seven, cyclical operation step 3 to six is carried out the domestication of pure bacterial strain 10 ~ 15 times; Eight, detect substratum 30ml and mix to containing the anaerobism that adds the step 1 preparation after the domestication in the 50mL centrifuge tube of bacterial strain, be filled with nitrogen 10 minutes in the centrifuge tube of 50mL, sealing places 17 ~ 21 ℃ shaking table to cultivate 1.5 ~ 2.5 hours; Nine, centrifuge tube is placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Ten, in the centrifuge tube of step 8 50mL, add aerobic detection substratum, cultivated 1.5 ~ 2.5 hours, the phosphorus concentration of cultivating phosphorus concentration in the bacterium liquid obtain with Ammonium Molybdate Spectrophotometric Method for Determination and being in b, the aerobic detection substratum is a, the dephosphorizing rate of pure bacterial strain is (1-b/a) * 100%, has namely determined the phosphorus removal property of biological phosphate-eliminating microorganism; Wherein the volume ratio of aerobic detection substratum and centrifuge tube is 0.5:1.11, the pure bacterial strain that step 9 is obtained carries out microscopy, determines that detecting the pure bacterial strain that obtains is the biological phosphate-eliminating microorganism.
In the culturing process of anaerobic culture medium, the polyphosphate particle in the biological phosphate-eliminating microorganism decomposer or glycogen release energy and absorb NaAc and form poly--beta-hydroxyl-alkanoates (PHAs); In the aerobic culture medium culturing process, the biological phosphate-eliminating microorganism decompose in vivo PHAs absorb phosphoric acid salt or glycogen biosynthesis as energy derive; Nutritive ingredient in the substratum of the present invention is reasonable, compare with the beef-protein medium of existing detection usefulness, the inventive method fully takes into account the growing environment of biological phosphate-eliminating microorganism in reactor, the dephosphorization ability appears in domestication biological phosphate-eliminating microorganism, and used detection substratum is fit to biological phosphate-eliminating microbial growth and domestication.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: the method for testing performance of pure bacterial strain carries out according to following steps: one, preparation anaerobism as claimed in claim 1 detects substratum, aerobic detection substratum; Two, get the bacterium liquid that 1mL separates the pure bacterial strain obtain and place the aerobic detection substratum of 50mL step 1 preparation, in 16 ~ 20 ℃ of shaking tables, cultivated 1 ~ 1.5 day, the bacterium liquid that again cultivation is obtained is poured in the centrifuge tube of 50mL, under 5000 ~ 6000 rev/mins condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Three, add the anaerobism detection substratum mixing that the 30ml step 1 is prepared again in the 50mL centrifuge tube, be filled with nitrogen 10 minutes in the 50mL centrifuge tube, sealing places 17 ~ 21 ℃ shaking table to cultivate 1.5 ~ 2.5 hours; Four, centrifuge tube is placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Five, add the aerobic detection substratum mixing that the 30ml step 1 is prepared again in the centrifuge tube of step 4 50mL, aeration is 1.5 ~ 2.5 hours at ambient temperature; Six, centrifuge tube is placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Seven, cyclical operation step 3 to six is carried out the domestication of pure bacterial strain 10 ~ 15 times; Eight, detect substratum 30ml and mix to containing the anaerobism that adds the step 1 preparation after the domestication in the 50mL centrifuge tube of bacterial strain, be filled with nitrogen 10 minutes in the centrifuge tube of 50mL, sealing places 17 ~ 21 ℃ shaking table to cultivate 1.5 ~ 2.5 hours; Nine, centrifuge tube is placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation; Ten, in the centrifuge tube of step 8 50mL, add aerobic detection substratum, cultivated 1.5 ~ 2.5 hours, the phosphorus concentration of cultivating phosphorus concentration in the bacterium liquid obtain with Ammonium Molybdate Spectrophotometric Method for Determination and being in b, the aerobic detection substratum is a, the dephosphorizing rate of pure bacterial strain is (1-b/a) * 100%, has namely determined the phosphorus removal property of biological phosphate-eliminating microorganism; Wherein the volume ratio of aerobic detection substratum and centrifuge tube is 0.5:1; 11, the pure bacterial strain that step 9 is obtained carries out microscopy, determines that detecting the pure bacterial strain that obtains is the biological phosphate-eliminating microorganism.
Every liter of anaerobism detection substratum is by the NaAc of 0.3 ~ 0.5g, (NH of 270 ~ 290mg in the present embodiment step 1 4) 2SO 4, 27 ~ 29mg CaCl 22H 2The MgSO of O, 350 ~ 370mg 47H 2The VITAMIN liquid of the liquid microelement of O, 0.5 ~ 0.7mL, 1.8 ~ 2.4mL and the distilled water of surplus form, and the pH value that anaerobism detects substratum is 6.9 ~ 7.4; Every liter of aerobic detection substratum is the KH by 70 ~ 80mg 2PO 4, 125 ~ 135mg K 2HPO 4, 260 ~ 300mg (NH 4) 2SO 4, 26 ~ 30mg CaCl 22H 2The MgSO of O, 340 ~ 380mg 47H 2The VITAMIN liquid of the liquid microelement of O, 0.5 ~ 1.2mL, 1.5 ~ 3mL and the distilled water of surplus form, and the pH value of aerobic detection substratum is 6.9 ~ 7.4.
Wherein anaerobism detects FeCl in the liquid microelement in substratum and the aerobic detection substratum 36H 2The concentration of O is 2.5 ~ 3.5g/L, H 3BO 3Concentration be 0.25 ~ 0.35g/L, CuSO 45H 2The concentration of O is that the concentration of 0.05 ~ 0.07g/L, KI is 0.32 ~ 0.4g/L, MnCl 24H 2The concentration of O is 0.2 ~ 0.28g/L, Na 2MO 42H 2The concentration of O is 0.1 ~ 0.14g/L, ZnSO 47H 2The concentration of O is 0.2 ~ 0.28g/L and CoCl 26H 2The concentration of O is 0.28 ~ 0.32g/L.The VITAMIN liquid that anaerobism detects in substratum and the aerobic detection substratum is the vitamins B of 80 ~ 120mg/L by concentration 1, concentration is the vitamins B of 80 ~ 120mg/L 2, concentration is 180 ~ 220mg/L vitamins B 6, concentration is the vitamins B of 1.8 ~ 2.2g/L 12, concentration is that folic acid, the concentration of 36 ~ 44mg/L is the nicotinic acid of 80 ~ 120mg/L, concentration is that the vitamin H that the calcium pantothenate of 80 ~ 120mg/L, para-amino benzoic acid that concentration is 80 ~ 120mg/L and concentration are 36 ~ 44mg/L forms.
In the 50mL centrifuge tube, add the aerobic detection substratum of 30ml in the present embodiment step 7, aeration is 1.5 ~ 2 hours at ambient temperature, sampling 0.6ml when cultivating 20 minutes, 40 minutes, 60 minutes, 90 minutes and 120 minutes respectively, place the 1.5ml centrifuge tube, 5000 ~ 6000 rev/mins after centrifugal 10 minutes, get respectively the 0.5ml supernatant liquor in another 1.5ml centrifuge tube, with the phosphorus concentration in the Ammonium Molybdate Spectrophotometric Method for Determination bacterium liquid, it is b that record detects the highest phosphorus concentration that obtains.
Embodiment two: what present embodiment and embodiment one were different is in the step 11 pure bacterial strain to be carried out microscopy, observe to determine that by microscopy it is tetramer form that embodiment four is separated the form that obtains pure bacterial strain, namely determine pure bacterial strain and be the fusca xylanase in the biological phosphate-eliminating microorganism.Other steps and parameter are identical with embodiment one.
The testing process of present embodiment is all carried out in Bechtop, and the anaerobism in the observation step 3 detects the variation of acetic acid in the substratum, phosphatic variation in the aerobic detection substratum in the step 4; Wherein remain on about 30mg in anaerobism cultivation stage acetic acid absorbed dose, all remain on about 3.8mg in aerobic cultivation stage phosphate absorption amount, the biological phosphate-eliminating microorganism is tamed in present embodiment step 3, four the culturing process, can discharge phosphoric acid salt at the anaerobism cultivation stage, and absorb phosphoric acid salt at aerobic cultivation stage; Present embodiment with strong points, the used substratum of present embodiment is fit to the domestication of biological phosphate-eliminating microorganism and the detection of dephosphorization ability.
Present embodiment is carried out Performance Detection and microscopy to 32 strain biological phosphate-eliminating microorganisms, and detected result is as shown in table 1, and wherein dephosphorizing rate is lower than 20% the non-dephosphorization microorganism that is.
Table 1
Kind Bacterial strain Dephosphorizing rate (%)
Acinetobacter sp. P38 8.9
Acinetobacter sp. P19 23
Acinetobacter sp. P4 100
Acinetobacter sp. P10 90.2
Acinetobacter sp. P43 28.5
Acinetobacter sp. P48 33.6
Acinetobacter sp. P23 41
Acinetobacter sp. P22 37.5
Acinetobacter sp. P13 98.4
Acinetobacter sp. P42 32
Acinetobacter sp. P47 42.8
Acinetobacter sp. P21 34.5
Acinetobacter sp. P33 65
Acinetobacter sp. P46 21.1
Delftia sp. P1 66
Delftia sp. P3 100
Delftia sp. P45 33
Delftia sp. P24 36.2
Delftia sp. P40 6.5
Delftia sp. P11 52.5
Delftia sp. P39 27.7
Delftia sp. P30 11.5
Pseudomonas sp. P2 97.3
Pseudomonas sp. P34 13.5
Pseudomonas sp. P12 72
Pseudomonas sp. P22 95.8
Pseudomonas sp. P7 92.5
Pseudomonas sp. P16 96
Aeromonas sp. P35 14.6
Brevibacillus sp. P26 84
Stenotrophomonas sp. P8 96.4
Paracoccus sp. P20 82.5
As can be seen from Table 1, the biological phosphate-eliminating microorganism dephosphorization of present embodiment domestication mechanism is machine-processed consistent with its dephosphorization in reactor, and dephosphorizing rate can reach 100%, and phosphor-removing effect is good.

Claims (10)

1. biological phosphate-eliminating microorganism detection substratum, it is characterized in that described detection substratum detect by the pure bacterial strain of biological phosphate-eliminating microorganism successively two kinds of substratum of usefulness, according to for detection of two kinds of substratum of order be respectively anaerobism and detect substratum, aerobic detection substratum; Wherein every liter of anaerobism detection substratum is by the NaAc of 0.3 ~ 0.5g, (NH of 270 ~ 290mg 4) 2SO 4, 27 ~ 29mg CaCl 22H 2The MgSO of O, 350 ~ 370mg 47H 2The VITAMIN liquid of the liquid microelement of O, 0.5 ~ 0.7mL, 1.8 ~ 2.4mL and the distilled water of surplus form, and the pH value that anaerobism detects substratum is 6.9 ~ 7.4; Every liter of aerobic detection substratum is the KH by 70 ~ 80mg 2PO 4, 125 ~ 135mg K 2HPO 4, 260 ~ 300mg (NH 4) 2SO 4, 26 ~ 30mg CaCl 22H 2The MgSO of O, 340 ~ 380mg 47H 2The VITAMIN liquid of the liquid microelement of O, 0.5 ~ 1.2mL, 1.5 ~ 3mL and the distilled water of surplus form, and the pH value of aerobic detection substratum is 6.9 ~ 7.4.
2. biological phosphate-eliminating microorganism detection substratum according to claim 1 is characterized in that component and the concentration in the liquid microelement in anaerobism detection substratum, the aerobic detection substratum is as follows: FeCl 36H 2The concentration of O is 2.5 ~ 3.5g/L, H 3BO 3Concentration be 0.25 ~ 0.35g/L, CuSO 45H 2The concentration of O is that the concentration of 0.05 ~ 0.07g/L, KI is 0.32 ~ 0.4g/L, MnCl 24H 2The concentration of O is 0.2 ~ 0.28g/L, Na 2MO 42H 2The concentration of O is 0.1 ~ 0.14g/L, ZnSO 47H 2The concentration of O is 0.2 ~ 0.28g/L and CoCl 26H 2The concentration of O is 0.28 ~ 0.32g/L.
3. biological phosphate-eliminating microorganism detection substratum according to claim 1 is characterized in that component and the concentration in the VITAMIN liquid in anaerobism detection substratum, the aerobic detection substratum is as follows: vitamins B 1Concentration be 80 ~ 120mg/L, vitamins B 2Concentration is 80 ~ 120mg/L, vitamins B 6Concentration be 180 ~ 220mg/L, vitamins B 12Concentration be that the concentration of 1.8 ~ 2.2g/L, folic acid is that the concentration of 36 ~ 44mg/L, nicotinic acid is 80 ~ 120mg/L, the concentration of calcium pantothenate is that the concentration of 80 ~ 120mg/L, para-amino benzoic acid is that the concentration of 80 ~ 120mg/L and vitamin H is 36 ~ 44mg/L.
4. the method for detection of biological dephosphorization microorganism dephosphorization ability is characterized in that the method for detection of biological dephosphorization microorganism dephosphorization ability is carried out according to following steps:
(1) preparation anaerobism as claimed in claim 1 detects substratum, aerobic detection substratum;
(2) get the aerobic detection substratum that the bacterium liquid that separates the pure bacterial strain that obtains places the step 1 preparation, cultivate, the bacterium liquid that again cultivation is obtained is poured in the centrifuge tube, and is centrifugal, abandons supernatant and stay precipitation;
(3) add the anaerobism detection substratum mixing that step 1 is prepared again in centrifuge tube, be filled with nitrogen in centrifuge tube, sealing places shaking table to cultivate;
(4) with centrifugal 10 ~ 15 minutes of centrifuge tube, abandon supernatant and stay precipitation;
(5) in the centrifuge tube of step (4), add the aerobic detection substratum mixing that step 1 is prepared again, at ambient temperature aeration;
(6) with centrifugal 10 ~ 15 minutes of centrifuge tube, abandon supernatant and stay precipitation;
(7) cyclical operation step (3)-(6) is carried out the domestication of pure bacterial strain 10 ~ 15 times;
(8) detect substratum and mix to containing the anaerobism that adds the step 1 preparation after the domestication in the centrifuge tube of bacterial strain, be filled with nitrogen in centrifuge tube, sealing places shaking table to cultivate;
(9) with centrifugal 10 ~ 15 minutes of centrifuge tube, abandon supernatant and stay precipitation;
(10) in the centrifuge tube of step (8), add aerobic detection substratum, cultivate, the phosphorus concentration of cultivating phosphorus concentration in the bacterium liquid obtain with Ammonium Molybdate Spectrophotometric Method for Determination and being in b, the aerobic detection substratum is a, the dephosphorizing rate of pure bacterial strain is (1-b/a) * 100%, has namely determined the phosphorus removal property of biological phosphate-eliminating microorganism; Wherein the volume ratio of aerobic detection substratum and centrifuge tube is 0.5:1;
(11) the pure bacterial strain that step (9) is obtained carries out microscopy, determines that detecting the pure bacterial strain that obtains is the biological phosphate-eliminating microorganism.
5. method according to claim 4, it is characterized in that, in the step (2): get the bacterium liquid that 1mL separates the pure bacterial strain obtain and place the aerobic detection substratum of 50mL step (1) preparation, in 16 ~ 20 ℃ of shaking tables, cultivated 1 ~ 1.5 day, the bacterium liquid that again cultivation is obtained is poured in the centrifuge tube of 50mL, under 5000 ~ 6000 rev/mins condition centrifugal 10 ~ 15 minutes, abandon supernatant and stay precipitation.
6. method according to claim 4, it is characterized in that, in the step (3): add the anaerobism detection substratum mixing that 30ml step (1) is prepared in the 50mL centrifuge tube, be filled with nitrogen 10 minutes in the 50mL centrifuge tube, sealing places 17 ~ 21 ℃ shaking table to cultivate 1.5 ~ 2.5 hours.
7. method according to claim 4 is characterized in that, in step (4), (6), (9): centrifuge tube placed under 5000 ~ 6000 rev/mins the condition centrifugal 10 ~ 15 minutes, and abandoned supernatant and stay precipitation.
8. method according to claim 4 is characterized in that together, in the step (5), adds the aerobic detection substratum mixing that 30ml step (1) is prepared in the centrifuge tube of step (4) 50mL, and aeration is 1.5 ~ 2.5 hours at ambient temperature.
9. method according to claim 4, it is characterized in that, mix to containing the anaerobism detection substratum 30ml that adds the step 1 preparation in the 50mL centrifuge tube of taming rear bacterial strain in the step (8), be filled with nitrogen 10 minutes in the centrifuge tube of 50mL, sealing places 17 ~ 21 ℃ shaking table to cultivate 1.5 ~ 2.5 hours.
10. method according to claim 4 is characterized in that the pure bacterial strain in the step (11) is carried out microscopy, observes by microscopy and determines that separating the form that obtains pure bacterial strain is tetramer form, determines that namely pure bacterial strain is the fusca xylanase in the biological phosphate-eliminating microorganism.
CN201210437874.4A 2012-11-06 2012-11-06 The method of biological phosphate-eliminating microorganism detection substratum and detection biological phosphate-eliminating microorganism dephosphorization ability Expired - Fee Related CN102911997B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223052A1 (en) * 2005-03-30 2006-10-05 Kimberly-Clark Worldwide, Inc. Technique for detecting microorganisms
WO2008118400A1 (en) * 2007-03-22 2008-10-02 Nanologix, Inc. Detection and identification of microorganisms on transparent permeable membranes
CN101560470A (en) * 2009-05-22 2009-10-21 哈尔滨工业大学 Culture medium for screening enhanced biological phosphorus removal functional bacteria and method for screening enhanced biological phosphorus removal functional bacteria

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223052A1 (en) * 2005-03-30 2006-10-05 Kimberly-Clark Worldwide, Inc. Technique for detecting microorganisms
WO2008118400A1 (en) * 2007-03-22 2008-10-02 Nanologix, Inc. Detection and identification of microorganisms on transparent permeable membranes
CN101560470A (en) * 2009-05-22 2009-10-21 哈尔滨工业大学 Culture medium for screening enhanced biological phosphorus removal functional bacteria and method for screening enhanced biological phosphorus removal functional bacteria

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