CN102998401A - Efficient environment-protection filling method for amino liquid chromatography column - Google Patents
Efficient environment-protection filling method for amino liquid chromatography column Download PDFInfo
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- CN102998401A CN102998401A CN2011102741781A CN201110274178A CN102998401A CN 102998401 A CN102998401 A CN 102998401A CN 2011102741781 A CN2011102741781 A CN 2011102741781A CN 201110274178 A CN201110274178 A CN 201110274178A CN 102998401 A CN102998401 A CN 102998401A
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- ethanol
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Abstract
The invention discloses an efficient environment-protection filling method for an amino liquid chromatography column, and relates to the field of biology and new medicine. The method comprises the following filling steps: exactly weighing a filler required by column tubes with different specifications, and adding the filler to a beaker; adding a certain amount of ethanol to carry out ultrasonic homogenizing so as to uniformly disperse the filler in the ethanol; transferring the homogenate liquid to a homogenate liquid tube, and adding ethanol until the height of the ethanol achieves the top of the tube; tightening a tube cap; carrying out slow pressurization until a flow rate is 4-5 ml, and stopping; maintaining stability for 50 min; starting to slowly release pressure, completing hydraulic pressure, and removing the column tube; and installing a column nut, and testing the filled liquid chromatography column. The filled liquid chromatography column has characteristics of high injection efficiency, good symmetry, good repeatability, stable performance, high recycling rate, saved cost and reduced environment pollution.
Description
Technical field:
The present invention relates to biological and new medicine field, be specifically related to a kind of high-efficiency environment friendly loading method of amino liquid-phase chromatographic column.
Background technology:
At present, it is high density homogenate method that the amino liquid-phase chromatographic column of generally acknowledging is loaded common method, employing be halogenated alkane as the homogenate agent, but the toxicity of the method homogenate agent itself is large, and the chromatographic column symmetry of loading is poor, and difficult recovery after the eluant, eluent in the filling process mixes, cost recovery make and load the cost rising greater than reagent cost, product at first loses competitive power on symmetric index and price, reagent can't reclaim, processing cost and all larger on the impact of environment, comparatively not environmental protection.
Summary of the invention:
The high-efficiency environment friendly loading method that the purpose of this invention is to provide a kind of amino liquid-phase chromatographic column, its adopts ethanol as the homogenate agent, and uses simultaneously ethanol to make eluant, eluent, corresponding pressure in addition on the column jecket of dimension, stablize certain hour under the certain flow rate, it is high that the chromatographic column of loading out is annotated effect, and symmetry is good, and repeatability is good, stable performance, and cyclic utilization rate is high, has not only saved cost, has reduced simultaneously the pollution to environment.
In order to solve the existing problem of background technology, the present invention takes following technical scheme: its filling step is: 1, accurately take by weighing the required amount of filler of different size column jecket in beaker, add the ultrasonic homogenate of a certain amount of ethanol, filler is uniformly dispersed in ethanol, shift homogenate to the homogenate pipe, and add ethanol to the pipe top, screw pipe cap, beginning is pressurization slowly, stops when being forced into flow velocity 4-5ml, keep stablizing 50 minutes, begin slow pressure release, hydraulic pressure is complete, takes off column jecket, add a cover the post nut, test loading complete liquid phase post.
The present invention has following beneficial effect: it adopts ethanol as the homogenate agent, and use simultaneously ethanol to make eluant, eluent, corresponding pressure in addition on the column jecket of dimension stablize certain hour under the certain flow rate, the chromatographic column of loading out is annotated the effect height, symmetry is good, repeatability is good, stable performance, and cyclic utilization rate is high, not only save cost, reduced simultaneously the pollution to environment.
Embodiment:
This embodiment is taked following technical scheme: its filling step is: 1, accurately take by weighing the required amount of filler of different size column jecket in beaker, add the ultrasonic homogenate of a certain amount of ethanol, filler is uniformly dispersed in ethanol, shift homogenate to the homogenate pipe, and add ethanol to the pipe top, screw pipe cap, beginning is pressurization slowly, stop when being forced into flow velocity 4-5ml, keep stablizing 50 minutes, begin slow pressure release, hydraulic pressure is complete, take off column jecket, add a cover the post nut, test loading complete liquid phase post.
Described column jecket specification and corresponding filler are: 250*4.6mm-3.5g, 150*4.6mm-2.0g, 100*4.6mm-1.5g, 50*4.6mm-1.1g, 150*2.1mm-1.0g.
The addition of the ethanol of described column jecket specification and correspondence is: 250*4.6mm-25ml, 150*4.6mm-15ml, 100*4.6mm-10ml, 50*4.6mm-10ml, 150*2.1mm-8ml.
Described column jecket specification and corresponding amount of pressurization are: 250*4.6mm-350bar, 150*4.6mm-320bar, 100*4.6mm-280bar, 50*4.6mm-230bar, 150*2.1mm-320bar.
This embodiment adopts ethanol as the homogenate agent, and use simultaneously ethanol to make eluant, eluent, corresponding pressure in addition on the column jecket of dimension stablize certain hour under the certain flow rate, the chromatographic column of loading out is annotated the effect height, symmetry is good, repeatability is good, stable performance, and cyclic utilization rate is high, not only save cost, reduced simultaneously the pollution to environment.
Claims (1)
1. the high-efficiency environment friendly loading method of an amino liquid-phase chromatographic column, the filling step that it is characterized in that it is: 1, accurately take by weighing the required amount of filler of different size column jecket in beaker, add the ultrasonic homogenate of a certain amount of ethanol, filler is uniformly dispersed in ethanol, shift homogenate to the homogenate pipe, and add ethanol to the pipe top, screw pipe cap, beginning is pressurization slowly, stops when being forced into flow velocity 4-5ml, keep stablizing 50 minutes, begin slow pressure release, hydraulic pressure is complete, takes off column jecket, add a cover the post nut, test loading complete liquid phase post.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102741781A CN102998401A (en) | 2011-09-15 | 2011-09-15 | Efficient environment-protection filling method for amino liquid chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102741781A CN102998401A (en) | 2011-09-15 | 2011-09-15 | Efficient environment-protection filling method for amino liquid chromatography column |
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| Publication Number | Publication Date |
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| CN102998401A true CN102998401A (en) | 2013-03-27 |
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| CN2011102741781A Pending CN102998401A (en) | 2011-09-15 | 2011-09-15 | Efficient environment-protection filling method for amino liquid chromatography column |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198831A (en) * | 2016-09-13 | 2016-12-07 | 吉尔生化（上海）有限公司 | A kind of method loading Reversed phase high performance liquid chromatography post |
| CN110694301A (en) * | 2019-11-05 | 2020-01-17 | 武汉谱立科技有限公司 | Liquid chromatographic column and preparation method thereof and preparation method of filler thereof |
| CN111239266A (en) * | 2018-11-29 | 2020-06-05 | 中国科学院大连化学物理研究所 | Thin-inner-diameter capillary liquid chromatographic column filling method and homogenizing tank |
Citations (3)
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|---|---|---|---|---|
| US4483773A (en) * | 1982-10-04 | 1984-11-20 | Varian Associates, Inc. | Narrow bore micro-particulate column packing process and product |
| US4483374A (en) * | 1982-11-01 | 1984-11-20 | Ethyl Corporation | Chromatographic column |
| CN101776668A (en) * | 2010-03-13 | 2010-07-14 | 兰州中科安泰分析科技有限责任公司 | Filling method of C18 HPLC (High Performance Liquid Chromatography) column |
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2011
- 2011-09-15 CN CN2011102741781A patent/CN102998401A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4483773A (en) * | 1982-10-04 | 1984-11-20 | Varian Associates, Inc. | Narrow bore micro-particulate column packing process and product |
| US4483374A (en) * | 1982-11-01 | 1984-11-20 | Ethyl Corporation | Chromatographic column |
| CN101776668A (en) * | 2010-03-13 | 2010-07-14 | 兰州中科安泰分析科技有限责任公司 | Filling method of C18 HPLC (High Performance Liquid Chromatography) column |
Non-Patent Citations (2)
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| 唐意红等: "不锈钢宽口径填充毛细管液相色谱柱", 《色谱》, vol. 19, no. 4, 31 July 2001 (2001-07-31) * |
| 谷雪: "新型毛细管液相色谱柱的研制及其相关应用", 《中国博士学位论文全文数据库工程科技Ⅰ辑》, no. 2, 15 August 2007 (2007-08-15), pages 014 - 131 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198831A (en) * | 2016-09-13 | 2016-12-07 | 吉尔生化（上海）有限公司 | A kind of method loading Reversed phase high performance liquid chromatography post |
| CN106198831B (en) * | 2016-09-13 | 2017-11-28 | 吉尔生化（上海）有限公司 | A kind of method for loading Reversed phase high performance liquid chromatography post |
| CN111239266A (en) * | 2018-11-29 | 2020-06-05 | 中国科学院大连化学物理研究所 | Thin-inner-diameter capillary liquid chromatographic column filling method and homogenizing tank |
| CN111239266B (en) * | 2018-11-29 | 2021-10-22 | 中国科学院大连化学物理研究所 | Thin-inner-diameter capillary liquid chromatographic column filling method and homogenizing tank |
| CN110694301A (en) * | 2019-11-05 | 2020-01-17 | 武汉谱立科技有限公司 | Liquid chromatographic column and preparation method thereof and preparation method of filler thereof |
| CN110694301B (en) * | 2019-11-05 | 2021-09-17 | 武汉谱立科技有限公司 | Liquid chromatographic column packing of metal-organic framework material and preparation method of chromatographic column |
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Address after: 201609 Shanghai Songjiang District Yexie town leaves 451 Lane 39, room 504, Zheng Lu Applicant after: Shanghai Anpu Experimental Technology Co., Ltd. Address before: 200030 Zhang Zemin Economic Development Zone, Shanghai, Songjiang District Applicant before: Shanghai ANPEL Scientific Instrument Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: SHANGHAI ANPEL SCIENTIFIC INSTRUMENT CO., LTD. TO: SHANGHAI ANPU EXPERIMENTAL TECHNOLOGY CO., LTD. |
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Application publication date: 20130327 |