CN103007404A - Method for establishing animal model of tracheostenosis and equipment thereof - Google Patents
Method for establishing animal model of tracheostenosis and equipment thereof Download PDFInfo
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- CN103007404A CN103007404A CN2012105314491A CN201210531449A CN103007404A CN 103007404 A CN103007404 A CN 103007404A CN 2012105314491 A CN2012105314491 A CN 2012105314491A CN 201210531449 A CN201210531449 A CN 201210531449A CN 103007404 A CN103007404 A CN 103007404A
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Abstract
The invention discloses a method for establishing an animal model of tracheostenosis and equipment thereof. The method comprises the steps of: selecting one intubation catheter with a sacculus; carrying out orotracheal intubation on an experimental animal, injecting gas in the sacculus and fixing the intubation catheter, wherein the air pressure injected into the sacculus is 175-225 mmHg, and the intubation catheter is kept for 18-25h; and pulling out the intubation catheter, wherein 1-3 weeks after the intubation catheter is pulled out, tracheostenosis is formed because of local granulation scar tissue proliferation and the tracheal collapse of the tracheal wall of the experimental animal. The invention also discloses the equipment for realizing the method. The method and the equipment, disclosed by the invention, have the advantages that simplicity and convenience are brought in operation, the repeatability is good and the real process of the generation of tracheostenosis of a clinical patient after endotracheal intubation is simulated, therefore the stable and reliable animal model of tracheostenosis for the research of benign airway stenosis is provided.
Description
Technical field
The present invention relates to field of medical technology, particularly a kind of method and equipment thereof of setting up the tracheal stenosis animal model by tracheal intubation.
Background technology
A kind of common complication that tracheal stenosis is the patient after tracheal intubation for a long time or improperly, tracheal stenosis also is one of type of the most common optimum airway constriction after the intubate.Occur after the intubate tracheal stenosis mainly too high with the gasbag pressure of tracheal casing pipe, intubation time is long and the factor such as intubate misoperation is relevant.The long-time pressurized of tracheal wall causes that myxedema is downright bad, ulcer even chondritis or chondronecrosis occur, finally cause local granulation scar tissue hypertrophy and (or) tracheal collapse and form tracheal stenosis.Although being widely used of the high capacitive catheter with cuff,endotracheal of low pressure, the phenomenon of tracheal stenosis after the intubate occurs in 19% patient that still has an appointment at present.Recent domestic has been attempted setting up the tracheal stenosis animal model with several different methods, mainly contain heating ablation method damage tracheal wall, tracheotomy stitching, machinery and chemical injury method etc., but damage the shortcomings such as roughly in a short time death of animal owing to its step complexity, poor repeatability, tracheal wall, and with the clinical patients intubate after the pathophysiological process difference of tracheal stenosis very large, be difficult to be used widely in the animal model of therefore above method foundation.
Be necessary to design a kind of method that can set up the tracheal stenosis animal model, with and special equipment solve the technical problem of above-mentioned proposition.
Summary of the invention
The first purpose of the present invention is to provide a kind of method that can set up the tracheal stenosis animal model, by its sacculus long period compression tracheal wall cause the tracheal wall tissue necrosis, come off, intracavity granulation (cicatrix) hyperproliferative even tube wall subside etc. causes luminal stenosis, thereby set up a kind of animal model of tracheal stenosis.
Another object of the present invention is to provide a kind of equipment of realizing the above-mentioned method of setting up the tracheal stenosis animal model.
In order to realize the first above-mentioned purpose, the present invention has adopted following technical scheme:
A kind of method of setting up the tracheal stenosis animal model, it may further comprise the steps,
S1, first-selection are selected an intubate conduit with sacculus;
S2, secondly, with described intubate conduit with sacculus to the laboratory animal orotracheal tube intubate of passing through;
S3, then, to described sacculus injecting gas and fixing intubate conduit, the air pressure that injects in the cuff is 175-225mmHg, and indwelling intubate conduit 18-25 hour;
S4, last carries out tube drawing with described tracheal intubation, and after tube drawing 1-3 week, because the local granulation scar tissue hypertrophy of laboratory animal tracheal wall and tracheal collapse finally form tracheal stenosis.
Further, in described step S3, during every 3 hours repetition measurement pressure and inflate 1 time to keep stable stress level at 200mmHg.
In order to realize the second above-mentioned purpose, the present invention has adopted following technical scheme:
A kind of equipment of setting up the tracheal stenosis animal model, it comprises the intubate conduit, be installed on the inflating balloon of intubate conduit one end, be used for syringe that described inflating balloon is inflated, and one be used for manometer that described inflating balloon is carried out pressure measurement, and described manometer and syringe are installed on the other end of intubate conduit.
Being shaped as after further, described inflating balloon is inflated is cylindrical.
Further, mercurial sphygmomanometer is counted in described pressure measurement.
Further, described manometer and syringe three-limb tube are connected with the intubate conduit.
The present invention has following beneficial effect: the method and equipment in use adopt the cooperation of intubate conduit, inflating balloon, manometer and syringe, simulate clinical patients and used the carcinogenesis of human that causes airway constriction after the tracheal intubation, easy and simple to handle, narrow real process, stable, reliable tracheal stenosis animal model occur for the research of optimum airway constriction provides a simulation clinical patients in good reproducibility.
Description of drawings
Fig. 1 is structure chart of the present invention;
Fig. 2 is the state diagram when setting up model of the present invention;
Fig. 3 is that the present invention is applied in the narrow general effects figure of isolated tracheal;
Fig. 4 is normal annulus trachealis and tracheal stenosis internal anatomy;
Fig. 5 is flow chart of the present invention.
Description of reference numerals:
1, laboratory animal, 2, the intubate conduit, 3, inflating balloon, 4, trachea, 5, manometer, 6, syringe, 7, the sacculus upper end, 8, the sacculus lower end, 9, the tracheal stenosis section, 10, normal annulus trachealis, 11, normal trachea chamber, 12, narrow trachea, 13, granulation tissue.
The specific embodiment
The present invention will be further described in detail below in conjunction with the drawings and specific embodiments.
Consult shown in Figure 1, in the invention provides a kind of equipment of setting up the tracheal stenosis animal model, it comprises intubate conduit 2, inflating balloon 3, manometer 5 and syringe 6, described inflating balloon 3 is installed on intubate conduit 2 one ends, described manometer 5 and syringe 6 are installed on the other end of intubate conduit 2, wherein syringe 6 is used for described inflating balloon is inflated, and manometer 5 is used for described inflating balloon is carried out pressure measurement; Being shaped as after described inflating balloon 3 inflations is cylindrical; Described manometer 5 is mercurial sphygmomanometer; Described manometer 5 and syringe 6 are connected with intubate conduit 2 through three-limb tube.
Consult shown in Figure 5ly, generally, the process of setting up the tracheal stenosis animal model of the present invention is:
First-selection is selected an intubate conduit with sacculus;
Secondly, with described intubate conduit with sacculus to the laboratory animal orotracheal tube intubate of passing through;
Then, to described sacculus injecting gas and fixing intubate conduit, the air pressure that injects in the cuff is 175-225mmHg, and indwelling intubate conduit 18-25 hour; During this time every 3 hours repetition measurement pressure and inflate 1 time to keep stable stress level at 200mmHg
At last, described tracheal intubation is carried out tube drawing, and after tube drawing 1-3 week, because the local granulation scar tissue hypertrophy of laboratory animal tracheal wall and tracheal collapse finally form tracheal stenosis.
In addition, the below can illustrate that the present invention sets up the process of tracheal stenosis animal model by intubate conduit 2 by a concrete example:
Consult shown in Fig. 2-4, selecting beasle dog is laboratory animal 1, (it can be the Parker Medical of GuangZhou, China Welllead company series to utilize band intubate conduit 2, model ID 8.0), cylindrical after inflating balloon 3 inflations, insert trachea 4 through intubate conduit 2, (sacculus upper end 7 is positioned at 3～4cm under the glottis to the insertion depth of adjustment intubate conduit 2, sacculus lower end 8 is apart from front tooth 28cm), conductive pipe inflating balloon 3 interior injecting gas are with fixing intubate conduit 2; Then utilize three-limb tube, intubate conduit 2, one ends that the one end connects connect manometer 5, one termination syringes 6.Then the air pressure that inject in the inflating balloon 3 are 200mmHg, kept somewhere in the intubate conduit 2 24 hours, during every 3 hours by manometer 5 repetition measurement pressure and inflate 1 time to keep stable stress level.By selecting the intubate conduit 2 than conventional caliber model bigger than normal, strengthen the pressure of inflating balloon 3, and indwelling enough time, Continuous Damage tracheal wall (such as the upper end 7 of the sacculus among Fig. 3 and sacculus lower end 8), myxedema necrosis, ulcer even cartilage ischemic necrosis (such as tracheal stenosis section 9 among Fig. 3) occur in tracheal wall in succession subsequently.Tube drawing is after 2 weeks, the local granulation scar tissue hypertrophy (granulation tissue 13 among Fig. 4) of compressing section tracheal wall and tracheal collapse, the final tracheal stenosis (the narrow trachea 12 among Fig. 3) that forms, can with Fig. 4 in normal annulus trachealis 10 and normal trachea chamber 11 form contrast.
Use the carcinogenesis of human that causes airway constriction after the tracheal intubation so that use the method and equipment can simulate clinical patients, easy and simple to handle, narrow real process, stable, reliable tracheal stenosis animal model occur for the research of optimum airway constriction provides a simulation clinical patients in good reproducibility.
Above-described embodiment is the better embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; and other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (6)
1. method of setting up the tracheal stenosis animal model is characterized in that: may further comprise the steps,
S1, first-selection are selected an intubate conduit with sacculus;
S2, secondly, with described intubate conduit with sacculus to the laboratory animal orotracheal tube intubate of passing through;
S3, then, to described sacculus injecting gas and fixing intubate conduit, the air pressure that injects in the sacculus is 175-225mmHg, and indwelling intubate conduit 18-25 hour;
S4, last pulls out described intubate conduit, after tube drawing 1-3 week, because the local granulation scar tissue hypertrophy of laboratory animal tracheal wall and tracheal collapse finally form tracheal stenosis.
2. the method for setting up the tracheal stenosis animal model according to claim 1 is characterized in that:
In described step S3, during every 3 hours repetition measurement pressure and inflate 1 time, to keep stable stress level at 200mmHg.
3. equipment that is used for realizing method claimed in claim 1, it is characterized in that: it comprises intubate conduit (2), is installed on the inflating balloon (3) of intubate conduit (2) one ends, be used for syringe (6) that described inflating balloon (3) is inflated, and one be used for manometer (5) that described inflating balloon (3) is carried out pressure measurement, and described manometer (5) and syringe (6) are installed on the other end of intubate conduit (2).
4. the equipment of setting up the tracheal stenosis animal model according to claim 3 is characterized in that: being shaped as after described inflating balloon (3) inflation is cylindrical.
5. the equipment of setting up the tracheal stenosis animal model according to claim 4, it is characterized in that: described manometer (5) is mercurial sphygmomanometer.
6. each described equipment of setting up the tracheal stenosis animal model according to claim 3-5, it is characterized in that: described manometer (5) and syringe (6) are connected with intubate conduit (2) through three-limb tube.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012105314491A CN103007404A (en) | 2012-11-23 | 2012-11-23 | Method for establishing animal model of tracheostenosis and equipment thereof |
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| CN2012105314491A CN103007404A (en) | 2012-11-23 | 2012-11-23 | Method for establishing animal model of tracheostenosis and equipment thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109350298A (en) * | 2018-12-20 | 2019-02-19 | 天津市胸科医院 | Realize the ventilation adjusting device of intermittent airway obstruction |
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| US4872483A (en) * | 1987-12-31 | 1989-10-10 | International Medical Products, Inc. | Conveniently hand held self-contained electronic manometer and pressure modulating device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109350298A (en) * | 2018-12-20 | 2019-02-19 | 天津市胸科医院 | Realize the ventilation adjusting device of intermittent airway obstruction |
| CN109350298B (en) * | 2018-12-20 | 2020-12-22 | 天津市胸科医院 | Ventilation regulating and controlling equipment for realizing intermittent airway obstruction |
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Application publication date: 20130403 |