CN103109866B - Light-efficiency accelerant for non-leaf organs of plants and preparation method thereof - Google Patents
Light-efficiency accelerant for non-leaf organs of plants and preparation method thereof Download PDFInfo
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- CN103109866B CN103109866B CN201310036522.2A CN201310036522A CN103109866B CN 103109866 B CN103109866 B CN 103109866B CN 201310036522 A CN201310036522 A CN 201310036522A CN 103109866 B CN103109866 B CN 103109866B
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Abstract
The invention discloses a light-efficiency accelerant for non-leaf organs of plants and a preparation method thereof. The light-efficiency accelerant is prepared from nanometer titanium dioxide, titanium dioxide nanotube, low sodium polyacrylate, potassium bicarbonate, dormin, brassinolide, potassium phosphate, sodium dodecyl sulfate, ethanol and sodium hydroxide according to proportions. The preparation method comprises the steps of: A. firstly dissolving the nanometer titanium dioxide and titanium dioxide nanotube in the ethanol, adding distilled water, carrying out ultrasonic concussion, adding low sodium polyacrylate, controlling under an aright constant temperature condition, using NaOH for adjusting pH, and preparing liquid A; B. dissolving potassium bicarbonate, dormin, brassinolide and potassium phosphate in the distilled water, and adjusting pH to obtain liquid B; and C. pouring the liquid A into the liquid B, stirring while pouring, adding sodium dodecyl sulfate to the mixture of the liquid A and the liquid B, placing at a constant temperature, and carrying out magnetic stirring to obtain the stable nanoscale small-bubble solution. The light-efficiency accelerant is reasonable in formula, convenient to use, free of environmental pollution and convenient to popularize and apply and has a direct effect and high utilization rate of nutrition.
Description
Technical field
The invention belongs to bioengineering field, more specifically relate to the non-foliage organ light efficiency of Plants promoter, also relate to the preparation method of the non-foliage organ light efficiency of Plants promoter simultaneously.This promoter is suitable for each breeding time of the various crop such as grain, cotton, oil, melon and fruit, flowers, promotes the increasing both production and income of these crops.
Background technology
Non-foliage organ, means except blade, and all organs that contains chloroplast, as parts such as stem stalk, fruit crust, fruit ear axles.As everyone knows, photosynthesis is the physiological foundation that crop yield forms, and the power of utility rate of luminous energy has determined the height of crop yield.But the current overwhelming majority's research all concentrates on the photosynthesis that how to improve plant leaf blade, also study very less for the photosynthesis of the non-foliage organ of plant, particularly Related product does not almost have especially.But increasing research shows, the non-foliage organ photosynthesis of plant is very huge to the contribution of output, as wheatear portion, stem stalk, fruit crust and lepicena reach 35-64% to the impact of output, the non-foliage organ photosynthesis such as rape stem, angle pericarp affect contribution rate 70% left and right to output.Find by the research to non-foliage organ photosynthetic pathway, they all have the blade of being different from C
3or C
4the feature of metabolic pathway, its photosynthetic rate is apparently higher than blade.Because non-foliage organ is as stem stalk, petiole etc. have the organic matter transportation systems such as screen casing, can efficiently shift its photosynthesis assimilation product in " storehouse ".Therefore, improve non-foliage organ photosynthesis and may cause the leap of crop yield and the green revolution of new generation.The development of China's light efficiency promoter is also in the starting stage at present, not only light efficiency promoter kind is little, and most of light efficiency promoter is all the transience light efficiency promotions based on leaf nutrition and increase blade carbon source, output to plant and economic benefit action effect can not be permanent, also need to spray repeatedly just to play certain effect simultaneously.The present invention passes through the first step: the photosynthesis enzyme of the non-foliage organ of regulation and control activation plant, second step: nutrition and the carbon source of supplying with the non-foliage organ necessity of plant; The 3rd step: form a kind of nanometer photosynthetic membrane on non-foliage organ surface, improve on the one hand the surface temperature of the non-foliage organ of rape, improve on the other hand non-foliage organ surface light scattering strength, and then improve the photosynthetic capacity of non-foliage organ.
Summary of the invention
The object of the invention is to be to provide the non-foliage organ light efficiency of Plants promoter, formula rationally, easy to use, can improve to greatest extent the photosynthetic efficiency of non-foliage organ.Simultaneously, because non-foliage organ has the features such as photosynthetic rate is high, large to output contribution, photosynthetic product transhipment is rapid, therefore with respect to blade light efficiency promoter, non-foliage organ light efficiency promoter has the advantages such as effect is direct, obvious effect of increasing production, economic coefficient is high, effect is lasting.
Another object of the present invention is the preparation method who has been to provide the non-foliage organ light efficiency of Plants promoter, and methodological science is easily gone, easy and simple to handle; Composition stably dispersing, nutrient utilization is high; Non-environmental-pollution, easy to utilize.
In order to realize above-mentioned object, the present invention adopts following technical measures:
The non-foliage organ light efficiency of one Plants promoter, it is made up of the mass fraction of following raw material:
The non-foliage organ light efficiency of one Plants promoter, it makes (preferable range) by the mass fraction of following raw material:
The non-foliage organ light efficiency of one Plants promoter, it makes (better scope) by the mass fraction of following raw material:
The non-foliage organ light efficiency of one Plants promoter, it makes (good scope) by the mass fraction of following raw material:
The non-foliage organ light efficiency of one Plants promoter, it makes (optimum range) by the mass fraction of following raw material:
The non-foliage organ light efficiency of one Plants promoter, it makes (optimum value) by the mass fraction of following raw material:
The preparation method of the non-foliage organ light efficiency of one Plants promoter, first dissolve the nano titanium oxide of 0.2-0.4 mass fraction with 95% ethanol of 1-2 mass fraction, the titania nanotube of 0.01-0.05 part mass fraction and sodium low polyacrylate 8.5-15.0 mass fraction, add the distilled water of 20-30 mass fraction, after ultrasonic oscillation 18-22min, airtight constant temperature control, after 20-24 hour, adjusts pH value to 8.0 to make A liquid with NaOH for subsequent use; Take the saleratus of 1.0-9.5 part quality, the dormin of 0.01-0.2 part quality, the study on brassinolide of 0.15-0.85 mass fraction, with the distilled water dissolving of 30-40 mass fraction, makes B liquid with the potassium phosphate adjustment pH value to 8.0 of 5-10 mass fraction for subsequent use.The 3rd step is slowly poured A liquid in B liquid, and limit bevelling stirs, and the sodium dodecyl that takes again 3-5 mass fraction after A liquid and B liquid are completely integrated adds wherein, is placed into and in constant temperature blender with magnetic force, stirs 20-24min.After dissolving completely, make non-foliage organ photosynthesis promoter mother liquor.Application concentration 500-1500ppm.Spray the time: seedling stage, elongation, florescence and fruiting period.
The steps include:
A, first dissolve nano titanium oxide and titania nanotube with 95% ethanol, add ultrasonic concussion 18-22min after 300ml distilled water, add sodium low polyacrylate, airtight constant temperature control 22-24 hour, NaOH adjusts pH value to 7.8-8.2 and is settled to 500ml, makes A liquid;
B, saleratus, dormin, study on brassinolide, potassium phosphate etc. are adjusted pH value to be settled to 500ml to 7.8-8.2 after dissolving with distilled water and are made B liquid;
C, then A liquid is slowly poured in B liquid, limit bevelling stirs, and A liquid and B liquid mix add sodium dodecyl completely, is placed into and in constant temperature blender with magnetic force, stirs 20-24min.Obtain overstable nanoscale vesicle solution.
From 2010, applicant successively patrols base, Wu Kong farm, Qianjiang, Aug. 1st farm, Qichun at Inst. of Oil Crops, Chinese Academy of Agriculture's sun, nearly more than 50 points are tested when the ground such as sun, From Zhongxiang Hubei, Suizhou, hubei, Nanchang, Yichuan, liuyang hunan complete altogether for Yaan, Sichuan, Wuhu, Chaohu, Anhui Province, China, Hubei, no matter professional research institution or farm, peasant household, participate in experimenter and all unanimously think, the present invention has the following advantages:
1. effect of increasing production is good.Product of the present invention, according to the international newest research results of current plant photosynthesis, adopts the aspect systematicness such as astigmatism, nutrition, carbon source, regulation and control to strengthen photosynthesis of plant, and photosynthetic efficiency improves more than 1.5 times than general light efficiency promoter; While can effectively ensure the abundance of plant organic matter and supply with fast, improves flowering of plant fruit-setting rate and Grain Filling degree, thus remarkable high yield.
Through 2011 ~ 2012 years two test years, successively exist, the crop of participating in the experiment comprises more than totally two ten kinds such as grain, economic crops, vegetables, test, demonstration area accumulative total reach more than 500 mu.The unit of participating in the experiment has carried out overall merit to 2011 ~ 2012 years test yield result:
(1) the comprehensive average amount of increase in production of cereal crops: soybean 2011 annual volume increase 37.6%; 2012 annual volume increase 44.8%; Paddy rice 2011 annual volume increase 18.1%; 2012 annual volume increase 19.6%; Corn 2011 annual volume increase 27.7%; 2012 annual volume increase 31.4%.
(2) economic crops: rape on average increases production 45%; Sesame on average increases production 40%; Beet on average increases production 32.1%; White melon on average increases production 43.1%; Stevia on average increases production 18.2%.
(3) 15 kinds of comprehensive average amounts of increase in production 17% ~ 68% such as vegetables, leaf vegetables, fruit and vegetable, tubers.
Taking in the rape variety of oil crop research institute of middle Academy of Agricultural Sciences seed selection two 11 as example: the data that after the non-foliage organ photosynthesis promoter of 2012 annual seedling spraying, elongation is measured as: spray after non-foliage organ photosynthesis promoter leaf area index and contrast and increase by 66.70%; Photosynthetic gesture contrasts increases by 39.96%; Net Photosynthetic Rate contrasts increases by 81.41%; When chlorophyll total amount: 1:500 doubly sprays process than contrast increase by 38.22%, 1:800 doubly spray process than contrast increase by 24.13%, 1:1000 doubly spray process than contrast increase by 14.82%.
2. significantly improve fruit quality.Product of the present invention, to regulate Crop Source base relation as basis, both provided crop needed balanced nutrient, can promote again flower bud differentiation of crop and bloom, and strengthened pollination; Promote fruit to form, alleviate physiological and non-physiologic shedding, and improve the sugar content of fruit; We show in the result of the test of changde, hunan, spray after this product, compared with the control, significantly improve quality of tobacco: cigarette strain leaf area significantly increases, tobacco leaf chemical composition total reducing sugar (28.66%), reducing sugar (22.71%), potassium content (2.76%), two sugar all reach sound tobacco standard index (with reference to " People's Republic of China (PRC) tobacco business standard, 2002 ") than (0.98) and sugared alkali than (9.08) etc. than (5.01), nitrogen base than (0.89), potassium chlorine.We show while 2011-2012 the result of the test of grape and pears, spray this product and can promote Fruit, significantly improve single fruit weight and output, promote mouthfeel, fruit type, fruit look, the glossy degree of fruit etc.
3, to thering is for studying thing the metabolic physiological function of adjusting, concrete manifestation make obviously to strengthen for the resistance that studies thing as: drought resistance, disease resistance; Promote root growth point growth rate in wound restore funcitons etc.Under drought condition, the result of the test on wheat, soybean (1:500 ~ 1000 times) is shown, spray that processing contrasts effective tiller, branch increases 1 ~ 3, leaf area increases more than 20%, can effectively control the fruit drop under adverse environmental factor simultaneously, promote fringe grain, pod grain to increase by 35% ~ 60%, thereby effectively reduce the impact of adverse circumstance on crop yield.
4. applicability is wide.Product of the present invention can be applied to grain, economic crops, vegetables etc. and more than totally two ten plant crop, all can use at seedling stage, elongation, the florescence of crop.Productive phase, also can be widely used in non-plant or the crop that eats or have the protection of pod skin such as cotton, rape, soybean.
5. the succinct science of formula, material source is wide, cost is low.Compound basis crop photosynthesis principle of the present invention, through great many of experiments optimization of C/C composites, formula is succinct and effect is remarkable; Raw materials all can be purchased acquisition on market, and the cost of raw material is low, source is wide.
6. have that anti-hard water does not flocculate, the particular advantages such as chemistry and biologically active height, be specially adapted to used as pesticides, chemical fertilizer potentiating and produce high-efficiency foliage fertilizer, growth regulator, soil conditioner, drought resistance agent, bio-fertilizer active carrier and other agricultural spraying dispenser agent etc.
Embodiment
Embodiment 1:
The non-foliage organ light efficiency of one Plants promoter, comprise 0.4 gram of nano titanium oxide (P25), 0.04 gram of titania nanotube, sodium low polyacrylate 12.0g, 7.5 grams of saleratus, 0.02 gram of dormin, 0.5 gram of study on brassinolide, 1.0 grams of potassium phosphates, sodium dodecyl 3.0g, 95% 2.0 grams of ethanol, 0.05 gram, sodium hydroxide, surplus is 73.49 grams of distilled water; Made by the mass fraction of following raw material:
Concrete preparation process is:
A, first dissolve nano titanium oxide and titania nanotube with 95% ethanol, add after 300ml distilled water ultrasonic concussion 18 or 19 or 20 or 21 or 22min, add sodium low polyacrylate, airtight constant temperature control 20 or 21 or 22 or 23 or 24 hours, NaOH adjusts pH value to 7.8 or 7.9 or 8 or 8.1 or 8.2 and be settled to 500ml, makes A liquid;
B, saleratus, dormin, study on brassinolide, potassium phosphate etc. are adjusted pH value to 7.8 or 7.9 or 8 or 8.1 or 8.2 to be settled to 500ml after dissolving with distilled water and are made B liquid;
C, then A liquid is slowly poured in B liquid, limit bevelling stirs, and A liquid and B liquid mix add sodium dodecyl completely, is placed into and in constant temperature blender with magnetic force, stirs 20 or 21 or 22 or 23 or 24min.Obtain overstable nanoscale vesicle solution.
Get this preparation 20ml seedling stage at sesame, add after 10 liters, clear water (diluting 500 times) mixes and evenly spray at noon on sesame stem and petiole, after 7 days, measure petiole photosynthetic rate, and after results, measure output.Photosynthesis effect is with to compare result as follows:
Note: utility rate of luminous energy=Net Photosynthetic Rate/intensity of illumination; Efficiency of water application=Net Photosynthetic Rate/transpiration rate.Lower same
Embodiment 2:
The non-foliage organ light efficiency of one Plants promoter, comprise 0.35 gram of nano titanium oxide (P25), 0.06 gram of titania nanotube, sodium low polyacrylate 10.0g, 8.0 grams of saleratus, 0.05 gram of dormin, 0.60 gram of study on brassinolide, 0.90 gram of potassium phosphate, sodium dodecyl 3.5g, 95% 1.5 grams of ethanol, 0.05 gram, sodium hydroxide, surplus is 74.99 grams of distilled water.Be that material quality mark is:
Preparation process is identical with embodiment 1.
Get this preparation 20ml at the soybean florescence, add after 10 liters, clear water (diluting 500 times) mixes and evenly spray at noon on soybean pod pericarp, stem and petiole, the knot angle phase is measured soybean pod pericarp photosynthetic rate, and after results, measures output.Photosynthesis effect is with to compare result as follows:
Embodiment 3:
The non-foliage organ light efficiency of one Plants promoter, comprise 0.3 gram of nano titanium oxide (P25), 0.05 gram of titania nanotube, sodium low polyacrylate 12.0g, 9.0 grams of saleratus, 0.1 gram of dormin, 0.5 gram of study on brassinolide, 1.2 grams of potassium phosphates, sodium dodecyl 3.5g, 95% 1.8 grams of ethanol, 0.05 gram, sodium hydroxide, surplus distilled water is 71.5 grams.Made by following material quality mark:
Preparation process is identical with embodiment 1.
Get this preparation 20ml at rape initial bloom stage, add after 16 liters, clear water (diluting 800 times) mixes and evenly spray at noon on siliqua of oilseed rape skin, stem, branch, the knot angle phase is measured siliqua of oilseed rape skin photosynthetic rate, and after results, measures output.Photosynthesis effect is with to compare result as follows:
Embodiment 4:
The non-foliage organ light efficiency of one Plants promoter, comprise 0.3 gram of nano titanium oxide (P25), 0.03 gram of titania nanotube, sodium low polyacrylate 12.5g, 7.0 grams of saleratus, 0.12 gram of dormin, 0.6 gram of study on brassinolide, 1.25 grams of potassium phosphates, sodium dodecyl 4.2g, 95% 1.4 grams of ethanol, 0.05 gram, sodium hydroxide, 72.55 grams of surplus distilled water.It is made up of the mass fraction of following raw material:
Preparation process is identical with embodiment 1.
Get this preparation 20ml at cotton flowering and boll-setting period, add after 20 liters, clear water (diluting 1000 times) mixes and evenly spray at noon on cotton bell, stem, branch, after 7 days, measure stem photosynthetic rate, and measure the rear output of results.Photosynthesis effect is with to compare result as follows:
Apply preparation of the present invention and carry out spraying test at seedling stage and the florescence of the crops such as rape, soybean, peanut, paddy rice, wheat and sesame respectively, the equal highly significant of effect of increasing production, particularly at the effect highly significant at florescence.
Seedling stage effect
Crop | Application method | Result of the test |
Rape | Seedling spraying, dilutes 800 times of liquid | Angle fruit number increases by 33%, and output increases by 42% |
Soybean | Seedling spraying, dilutes 500 times of liquid | Pod number increases by 41%, and output increases by 49% |
Peanut | Seedling spraying, dilutes 500 times of liquid | Pod number increases by 20%, and output increases by 32% |
Cotton | Seedling spraying, dilutes 800 times of liquid | Peach bell number increases by 19%, 31% |
Sesame | Seedling spraying, dilutes 500 times of liquid | Capsule number increases by 24, and output increases by 41% |
Florescence effect
Crop | Application method | Result of the test |
Rape | Spraying in the flowering stage, dilutes 800 times of liquid | Output increases by 56% |
Soybean | Spraying in the flowering stage, dilutes 1000 times of liquid | Output increases by 68% |
Peanut | Spraying in the flowering stage, dilutes 800 times of liquid | Output increases by 44% |
Cotton | Spraying in the flowering stage, dilutes 800 times of liquid | Output increases by 62% |
Sesame | Spraying in the flowering stage, dilutes 1000 times of liquid | Output increases by 39% |
Claims (7)
7. the preparation method of the non-foliage organ light efficiency of a Plants claimed in claim 1 promoter, the steps include:
A, first dissolve nano titanium oxide P25 and titania nanotube with 95% ethanol, add ultrasonic concussion 18-22min after 300ml distilled water, add sodium low polyacrylate, airtight constant temperature is placed 22-24 hour, NaOH adjusts pH value to 7.8-8.2 and is settled to 500ml, makes A liquid;
B, saleratus, dormin, study on brassinolide, potassium phosphate are adjusted pH value to be settled to 500ml to 7.8-8.2 after dissolving with distilled water and are made B liquid;
C, then A liquid is slowly poured in B liquid, limit bevelling stirs, and A liquid and B liquid mix add sodium dodecyl completely, are placed in constant temperature blender with magnetic force and stir 20-24min, obtain overstable nanoscale vesicle solution.
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CN103641636B (en) * | 2013-12-21 | 2015-02-11 | 李俊 | Nano light carbon agent and preparation method thereof |
CN107306994B (en) * | 2017-07-17 | 2020-06-09 | 陈菁 | Nano plant photosynthesis promoter and preparation method and application thereof |
CN110771625A (en) * | 2019-09-17 | 2020-02-11 | 江苏新南洋绿色科技有限公司 | Wettable agent formula for enhancing high-temperature stress resistance of crops |
CN114467730B (en) * | 2022-02-25 | 2022-11-15 | 安徽农业大学 | Method for increasing contents of nicotinamide and trehalose of non-heading Chinese cabbage |
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