CN103141418A - In-vitro long-acting labeling method applicable to stichopus japonicus - Google Patents
In-vitro long-acting labeling method applicable to stichopus japonicus Download PDFInfo
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- CN103141418A CN103141418A CN2013100730067A CN201310073006A CN103141418A CN 103141418 A CN103141418 A CN 103141418A CN 2013100730067 A CN2013100730067 A CN 2013100730067A CN 201310073006 A CN201310073006 A CN 201310073006A CN 103141418 A CN103141418 A CN 103141418A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to the technical field of aquaculture, in particular to an in-vitro long-acting labeling method applicable to stichopus japonicas. The method comprises the following steps: penetrating a labeling line from the mouth of the stichopus japonicas; after penetrating a lime ring, penetrating out of the body from the position above the head of the stichopus japonicas; fixing a tag capable of identifying the stichopus japonicas on the penetrated labeling line; and stably closing the labeling line to realize the in-vitro long-acting labeling of the stichopus japonicas. By adoption of the labeling method, the stichopus japonicas individual can maintain the in-vitro tag for more than 3 months, the normal activity of the stichopus japonicas individual is influenced hardly, and various field labeling release tests and other work which needs to accurately identify the stichopus japonicas individual after the stichopus japonicas individual is labeled can be further developed.
Description
Technical field
The present invention relates to technical field of aquaculture, specifically a kind of external long-acting labeling method that is applicable to stichopus japonicus.
Background technology
Stichopus japonicus is one of important mariculture kind of China.Usually to carry out mark and Tracking Recognition to it in aquaculture production, prevalent variety cultivation and the scientific experiment process of stichopus japonicus.In numerous biomarker methods, external tracer method is the method for the identification marking biology of the most directly perceived, most convenient, but because the stichopus japonicus body wall is soft, whole body is several without hard structure, and stichopus japonicus has very strong rejection ability, therefore is difficult to the external labeling method of other marine organisms (as fish) is applied on stichopus japonicus.The external labeling method of success must satisfy two conditions: mark can be retained for a long time; Being labeled biology can keep normal behaviour movable.Pre-stage test shows, adopting after external label 1-2 days that conventional body wall puncture method or mounting method hang in its body surface system just can self falling, and very large to the stichopus japonicus damage.Other molecular labeling method and osteocomma fluorescent staining tracer method are arranged, but its shortcoming is on-the-spot None-identified for the labeling method of stichopus japonicus, must gather individuality and detect by laboratory experiment or use special inspecting equipment afterwards.Therefore, need a kind of stichopus japonicus, reliable and stable external labeling method of being applicable to of exploitation badly, this will provide important technical support for census, stock enhancement effect tracking and monitoring and the praxiology research of carrying out stichopus japonicus.Calcareous ring is the peculiar calcareous organ of stichopus japonicus, is positioned at that it is pharyngeal, and the organs such as, nerve ring pharyngeal for supporting and ring water pipe play an important role, and its outstanding feature is closed hard loop configuration.If utilize this structure, develop the external labeling method of a kind of long-acting stichopus japonicus just very hopeful.
Summary of the invention
The object of the invention is a kind of external long-acting labeling method that is applicable to stichopus japonicus.
For achieving the above object, the technical solution used in the present invention is:
The present invention utilizes calcareous ring as external mark system's extension position, has captured the external mark of stichopus japonicus and has been difficult to a long-term difficult problem of retaining; The present invention has stipulated marking operation equipment and necessary technical parameter simultaneously, has guaranteed that the stichopus japonicus after the mark can survive and normally ingest.
Be specially:
A kind of external long-acting labeling method that is applicable to stichopus japonicus, mark line is penetrated and pass after its calcareous ring from the oral area of stichopus japonicus to be passed external in stichopus japonicus above-head position, then fix the label that to identify stichopus japonicus on the mark line that passes, make at last the mark line secure closure, namely realize the external long-acting mark of stichopus japonicus.
Described stichopus japonicus for mark should be selected disease-free, undamaged healthy stichopus japonicus.
Describedly mark line is 45° angle from the oral area of stichopus japonicus and its health direction penetrates, pass after its calcareous ring in its above-head position and pass, tie a knot after fixing suitable label on mark line.
After described mark line closure, mark line to the maximum normal distance of stichopus japonicus body wall less than 0.5cm.Described fixing label is that weight is less than the label of the total weight in wet base 5% of stichopus japonicus.The pin that described use is marked is that diameter is less than sewing-needle, embroidery needle or other hard pin of 0.4mm.Described mark line can be wire diameter and has some strength, ganoid line less than the nylon setline of 0.2mm, operation suture thread or other.
The invention has the beneficial effects as follows:
1, adopt the present invention, the stichopus japonicus label can be retained for a long time, has avoided being discharged by stichopus japonicus, and stichopus japonicus can keep its external label more than at least 3 months, solved the difficult problem that stichopus japonicus is difficult to carry out long-term external mark, and the stichopus japonicus of mark can keep normal feeding activity.
2. employing the present invention, can be the different types of external identification label of stichopus japonicus system's extension, as electronic tag, color label, acoustics label etc., can further carry out release experiment and other of various open-air marks after the stichopus japonicus mark and need accurately work of identification stichopus japonicus, as mark recapture, parentage identification and behaviouristics tracking etc., broken and carried out the technical bottleneck that above work faces.
3. the present invention has wider applicability, applicable in Holothuroidea, all have the kind of closed calcareous ring structure.
Description of drawings
The external labeling method schematic diagram that is applicable to stichopus japonicus that Fig. 1 provides for the embodiment of the present invention.In figure, 1 label; 2 mark lines; 3 stichopus japonicus oral areas
Embodiment
A kind of external long-acting labeling method that is applicable to stichopus japonicus, the stichopus japonicus that is used for mark should be selected disease-free, undamaged healthy stichopus japonicus.Less than the fine needle of 0.4mm, mark line 2 is 45° angle from the oral area 3 of stichopus japonicus with its health direction with diameter and penetrates, pass after its calcareous ring in its above-head position and pass, fix the rear knotting of suitable label 1 on mark line.After the mark line closure apart from the body wall maximum distance less than 0.5cm.Mark line 2 can be thin ganoid nylon setline, operation suture thread or other has the line of some strength.Label 1 weight fixing on mark line 2 is less than 5% of the total weight in wet base of stichopus japonicus, can be used for the label of individual identification for common color label, electronic tag or other.
Embodiment 1: adopt nylon setline, color plastic label stichopus japonicus
Select the nylon setline of the 0.16mm diameter line that serves as a mark, with the stichopus japonicus of plastic label (weight is 1g) the mark body weight 100g left and right of length 8mm different colours.With label through on mark line, be that the embroidery needle of 0.35mm is 45° angle from the oral area of stichopus japonicus and its health direction and penetrates with diameter, pass after its calcareous ring in its above-head position and pass, mark line was drawn calcareous ring, adjust afterwards the knotting position, make linear distance stichopus japonicus body wall be no more than 0.5cm, with electric iron with the line sintering, cut off the end of a thread, the label installation.The stichopus japonicus label of hanging by the party's genealogy of law fell the label rate lower than 10% in 3 days, and the label of reservation can firmly be retained more than 3 months, and the stichopus japonicus of mark just can recover feeding activity after 12 hours, reached normal food ration after 3 days.The label of different colours is convenient to identification under water in the sea area, diver's searching and the stichopus japonicus of recapturing are very convenient.
Embodiment 2: adopt monofilament polypropylene operation suture thread, PIT electronic tag mark stichopus japonicus
Select the monofilament polypropylene operation suture thread line that serves as a mark, PIT electronic tag (weight is 2g) and mark line that will long 6mm, diameter 1mm with one little section plastic bushing be fixed together, the stichopus japonicus of mark 100g left and right.Be that the embroidery needle of 0.35mm is 45° angle with the nonabsorable operation suture thread from the oral area of stichopus japonicus and its health direction and penetrates with diameter, pass after its calcareous ring in its above-head position and pass, mark line was drawn calcareous ring, adjust afterwards the position of tiing a knot, make linear distance stichopus japonicus body wall be no more than 0.5cm, manual knotting is cut off the end of a thread, the label installation.The stichopus japonicus label of hanging by the party's genealogy of law fell the label rate lower than 13% in 4 days, and the label of reservation can firmly be retained more than 3 months, and the stichopus japonicus of mark just can recover feeding activity after 12 hours, reached normal food ration after 3 days.This label is equipped with special-purpose barcode scanning device, and each label has unique electronic code, is convenient to identification.Sweep record coding before stichopus japonicus is thrown in, scanning again after recapture can obtain the significant datas such as population density, individual growth of stichopus japonicus.
The embodiment result shows, adopts mark stichopus japonicus of the present invention convenient, reliable, is convenient to identification, is the effective ways that are applicable to stichopus japonicus or the external mark of other sea cucumber.
Claims (7)
1. external long-acting labeling method that is applicable to stichopus japonicus, it is characterized in that: mark line is penetrated and pass after its calcareous ring from the oral area of stichopus japonicus pass external in stichopus japonicus above-head position, then fix the label that to identify stichopus japonicus on the mark line that passes, make at last the mark line secure closure, namely realize the external long-acting mark of stichopus japonicus.
2. by the external long-acting labeling method that is applicable to stichopus japonicus claimed in claim 1, it is characterized in that: described stichopus japonicus for mark should be selected disease-free, undamaged healthy stichopus japonicus.
3. by the external long-acting labeling method that is applicable to stichopus japonicus claimed in claim 1, it is characterized in that: describedly mark line is 45° angle from the oral area of stichopus japonicus and its health direction penetrates, pass after its calcareous ring in its above-head position and pass, tie a knot after fixing suitable label on mark line.
4. by the described external long-acting labeling method that is applicable to stichopus japonicus of claim 1 or 3, it is characterized in that: after described mark line closure, mark line to the maximum normal distance of stichopus japonicus body wall less than 0.5cm.
5. by the described external long-acting labeling method that is applicable to stichopus japonicus of claim 1 or 3, it is characterized in that: described fixing label is that weight is less than the label of the total weight in wet base 5% of stichopus japonicus.
6. by the described external long-acting labeling method that is applicable to stichopus japonicus of claim 1 or 3, it is characterized in that: the pin that described use is marked is that diameter is less than sewing-needle, embroidery needle or other hard pin of 0.4mm.
7. by the described external long-acting labeling method that is applicable to stichopus japonicus of claim 1 or 3, it is characterized in that: described mark line can be wire diameter and has some strength, ganoid line less than the nylon setline of 0.2mm, operation suture thread or other.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103749359A (en) * | 2014-01-09 | 2014-04-30 | 大连海宝渔业有限公司 | Sea urchin individual marking method |
| CN106561525A (en) * | 2016-11-07 | 2017-04-19 | 浙江省海洋水产研究所 | Fish tag label, fish tag device and labeling method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103749359A (en) * | 2014-01-09 | 2014-04-30 | 大连海宝渔业有限公司 | Sea urchin individual marking method |
| CN106561525A (en) * | 2016-11-07 | 2017-04-19 | 浙江省海洋水产研究所 | Fish tag label, fish tag device and labeling method |
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| CN103141418B (en) | 2014-04-23 |
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