CN103191479A - Optimization method for continuous centrifugal blood separation in curved-surface container - Google Patents

Optimization method for continuous centrifugal blood separation in curved-surface container Download PDF

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CN103191479A
CN103191479A CN2012100035800A CN201210003580A CN103191479A CN 103191479 A CN103191479 A CN 103191479A CN 2012100035800 A CN2012100035800 A CN 2012100035800A CN 201210003580 A CN201210003580 A CN 201210003580A CN 103191479 A CN103191479 A CN 103191479A
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blood
separation
soft bag
curve
separates
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CN103191479B (en
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经建中
刘继纯
郑佳敏
唐海波
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GOLDEN MEDITECH (SHANGHAI) CO Ltd
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GOLDEN MEDITECH (SHANGHAI) CO Ltd
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Abstract

The invention relates to an optimization method for continuous centrifugal blood separation in a curved-surface container. The method comprises the following steps: a separation cavity gap is formed in a container, and adopts the structure of a curved surface; a separation soft bag is arranged inside the separation cavity gap; blood is fed into the separation soft bag, and is led to rotate at high speed around an rotation axle along with the curved-surface container; different blood ingredients are separated from the blood as per density under the action of a centrifugal force; the continuous centrifugal blood separation is performed in the separation soft bag; and the rotation direction of the curved-surface container is the same as the unfolding direction of the separation cavity gap. According to the method provided by the invention, the separation soft bag and the curved-surface container are used together, and the direction of centrifugal rotation is controlled, so that the continuous centrifugal separation of the blood ingredients is realized; and the method has the optimal advantage of capability of increasing the separation quality without increasing separation cost.

Description

A kind of blood optimization method that continuous centrifugal separates in the curved body container
Technical field
The present invention relates to blood constituent and separate, particularly relate to and a kind ofly blood is carried out continuous centrifugal separate, thereby isolate the optimization method of visible components such as blood plasma and blood cell.
Background technology
No matter be scientific research or clinical medicine practice or commercial production, and more conditions, all need blood separation, as going out single component from separation of whole blood, usually use centrifuging from blood, to isolate various single components, be used for occasions such as clinical treatment, scientific research or preparation raw material.Modal is that whole blood is isolated erythrocyte, granulocyte, mononuclear cell, platelet and blood plasma by centrifugal separation system, and erythrocyte and cleaning mixture are isolated in the FRC washing after maybe will thawing.
The operation principle of continuous centrifugal separation system is: the primary structure of said system includes centrifuge, infusion pump and control device; By the infusion pipeline that is connected with infusion pump blood is introduced on the centrifuge in the soft bag in the knock-out drum, the high speed rotating knock-out drum, and this soft bag synchronous high-speed rotation of drive, the not same-action that blood in it is subjected to centrifugal force field causes visible components such as blood plasma and blood cell to do the centrifugal sedimentation campaign, and by the big or small layering of density or proportion or sedimentation coefficient separately; When reaching sedimentation equilibrium, arrange each single component layer of forming concentric circles by the density height to low enrichment to the axle center from periphery radially, utilize infusion pump again the single component layer that separates to be extracted then.
The blood single component realizes that in this system continuous acquisition is rotary power to be provided and to play the effect of continuous input and extraction by airtight flexible pipe, the soft bag that separates in one end of flexible pipe and the knock-out drum is communicated with, with the knock-out drum high-speed rotation, the other end of flexible pipe is fixed on the support, therefore, flexible pipe one end rotates an end to be fixed, there is in the middle of it coil arrangement to realize that the flexible pipe solution of untwisting twines, make under rotation status, whole blood to be inputed in the knock-out drum of rotation, and in the knock-out drum of rotation, extract single components such as blood plasma and blood cell out.Knock-out drum, i.e. separator disk combines with coil arrangement and to have realized that the blood continuous centrifugal separates.
What relate to the separator disk that is applied on the blood continuous centrifugal separation equipment and coil arrangement in the prior art mainly contains US Patent No. 5360542.In this patent, separator disk be one columnar structured, be called knock-out drum, a columnar lacuna is arranged in it, soft bag is placed on the centrifugalize that realizes blood in the circular lacuna; Coil arrangement includes underframe, rotatable top-support suspends knock-out drum in midair on the top-support, flexible pipe extend into the knock-out drum bottom fixedly pass two bearings of top-support side from the cabinet place after, the end of its flexible pipe is a square spigot, extend in the square groove of knock-out drum central axis.Flexible pipe is hollow structure, and several transfer pipelines are arranged in it, realizes that simultaneously power provides and the liquid conveying function.Based on above-mentioned structure, power makes top-support rotation, drives flexible pipe and untwists and produce torque forces, and this power is transferred to knock-out drum make it to produce in the same way and rotate, and then realize the separation process of blood continuous centrifugal.
Disclosed the separator disk of another version in the Chinese patent application 200710046991.7.Point out in this patent: the separator disk on the many cells composition mixing material piece-rate system, comprise durable hard-caked sediment dish and disposable soft bag, the circular discs of being formed by inner core and base on the hard-caked sediment dish, between inner core and base, form one section around continuous lacuna this hard-caked sediment one week of dish circle core shaft and that stem and afterbody do not seal, soft bag is for being provided with single cavity configuration of liquid inlet and outlet pipe, and this soft bag can be inserted in the described lacuna.Because everywhere centrifugal force is inconsistent in the described continuous lacuna, therefore under the centrifugal force continuous action, each composition of mixing material is the segmentation distribution in soft bag, can extract corresponding single component in above-mentioned segmentation.Above-mentioned inner core is equivalent to described interior cylinder, base is equivalent to described outer cylinder.Above-mentioned hard-caked sediment dish that is to say separator disk.
No matter be foreign patent or domestic patent, all adopted separator disk to add the mode of soft bag, soft bag is disposable consumptive material parts.Owing to the mentality of designing difference of blood separation, the former is concentric design to the separator disk of these two kinds of forms in actual use, and the latter is non-concentric design, and the latter's separation efficiency improves a lot than the former.Though the latter is comparatively ingenious with the design that separates soft bag at hard-caked sediment dish, and certain effect is arranged in practice, but still the leeway of the design of improving is arranged.
Summary of the invention
The objective of the invention is to overcome the deficiency that above-mentioned prior art exists the optimization method that provides a kind of new blood continuous centrifugal to separate.Method of the present invention by controlling centrifugal direction of rotation, does not increase separation costs, and improves the quality of separated component in being intended to realize that the blood continuous centrifugal separates efficiently.
In order to reach the foregoing invention purpose, technical scheme provided by the invention is as follows:
A kind of blood optimization method that continuous centrifugal separates in the curved body container, it is characterized in that, be provided with the separation lacuna in the described curved body container, this curved body container is around the rotating shaft high speed rotating, and the blood that utilizes centrifugal force will be injected in the described separation lacuna goes out heterogeneity by the density size separation; Described separation lacuna is the curved body that includes outer wall and inwall, and this outer wall curved surface that separates lacuna curve of projection on perpendicular to the plane of rotating shaft is as follows with polar coordinate representation:
Figure 452008DEST_PATH_IMAGE001
Wherein, polar limit O is the intersection point on rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise direction, r is any utmost point of some footpath on the curve, and R is the utmost point footpath of curve distal-most end, θ 1Be the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ 2Be curve and separating plasma factor critical radius F rThe polar angle of intersection point, separating plasma factor critical radius F rThe required minimum centrifugal radius of separating plasma in unit interval when determining for rotating speed, θ 3Be a polar angle, its value is θ 21.5~3.5 times, θ 4Polar angle for the curve distal-most end; In described separation lacuna, [θ 1, θ 2) be the blood plasma district, [θ 2, θ 3) be the chaotic region, [θ 3, θ 4] be the blood cell district, b 1Be the curve coefficients in blood plasma district, b 2Be the curve coefficients of chaotic region, b 3Curve coefficients for the blood cell district; The direction of rotation of described separation container is identical with the expansion direction that separates lacuna.
In the blood of the present invention optimization method that continuous centrifugal separates in the curved body container, described curve coefficients refers to that the utmost point footpath of any point on the curve is with respect to the rate of change of polar angle.
In the blood of the present invention optimization method that continuous centrifugal separates in the curved body container, the expansion direction of described separation lacuna refers to: in the blood plasma district of described curve, any 2 p and q are arranged, its polar angle is respectively θ p and θ q, the corresponding utmost point directly is respectively rp and rq, and θ 1≤ θ p<θ q≤θ 2, while rp<rq, some p is described curve expansion direction along the direction that curvilinear path moves to a q.
In the blood of the present invention optimization method that continuous centrifugal separates in the curved body container, the continuous centrifugal separation process of described blood specifically comprises the steps:
The first step, the described curved body container of high speed rotating, place the soft bag of the separation that separates in lacuna high speed rotating thereupon, front portion, middle part, the rear portion of the soft bag of this separation is respectively equipped with a flexible pipe of carrying liquid, the front portion of separating soft bag is positioned at the disengagement chamber crack near-end nearer apart from rotating shaft, the middle part of separating soft bag is positioned at the middle part of separating lacuna, and the rear portion of separating soft bag is positioned at the disengagement chamber crack far-end far away apart from rotating shaft;
Second step, blood is injected into the soft bag of separation by being positioned at the flexible pipe that separates soft bag middle part, after treating that whole blood is full of the soft bag of separation space, separate soft bag and separate lacuna and fit tightly formation and separate the inside separated space that lacuna is close, blood is subjected under the centrifugal force continuous action various compositions segmentation and regionalization gradually in separating soft bag, blood plasma accumulates in separation soft bag front portion gradually and forms a blood plasma district, comprise blood cell and accumulate in separation soft bag rear portion gradually and form a blood cell district in interior visible component, and separate the middle part of soft bag because the lasting input of whole blood forms a chaotic region;
The 3rd step, separate the anterior flexible pipe of soft bag at blood plasma district extraction blood plasma from being arranged on, extraction comprises blood cell in interior visible component in the blood cell district from being arranged on the flexible pipe that separates soft bag rear portion, continue the input whole blood from being arranged on the flexible pipe that separates soft bag middle part simultaneously, reach the dynamic equilibrium that separates soft bag inner capacities by the control input with extracting out, and then realize that the continuous centrifugal of blood separates.
Based on the foregoing invention content, the present invention carries out in the method that continuous centrifugal separates comparing with method of the prior art to blood and has following technique effect:
1. the present invention is in order to realize the continuous high-efficient centrifugalize of blood, the concept that the curved surface continuous centrifugal separates has been proposed, and designed the curved body container that a suitable continuous centrifugal separates based on this thought, this curved body container is embodied in the separation lacuna of a separator disk the inside, in separating lacuna, arrange one and separate soft bag with the inside and outside wall that separates lacuna fits, and separating the flexible pipe that soft bag arranges input and extracts liquid out, the flexible pipe that extracts heterogeneity is arranged in the different subregions, a total input pipe is set again, blood placed in this soft bag carry out high speed rotating, under action of centrifugal force, blood can carry out segmentation and regionalization according to the density difference in the separated space that curved surface constitutes, can realize the continued operation of blood separation by input and the dynamic equilibrium of extracting out like this, improve the separation efficiency of blood.
2. the present invention is for the purpose of the optimization continuous high-efficient centrifugalize that reaches blood, design and pass through the direction of rotation of control continuous curve surface body container, namely the direction of rotation of this container is identical with the expansion direction that separates lacuna, realizes not increasing separation costs, and improves the quality of separated product.
Description of drawings
Fig. 1 separates the projection of lacuna curved surface and the sketch map of direction of rotation in the optimization method that separates of blood continuous centrifugal of the present invention.
Fig. 2 is the structural representation of centrifugal separation system.
Fig. 3 is the structural representation of separator disk among the present invention.
Fig. 4 is the deployed configuration sketch map that separates soft bag among the present invention.
Fig. 5 is that the present invention separates the sketch map that flows to that blood in the soft bag has just entered separated space.
Fig. 6 is that the present invention separates blood in the soft bag and is full of the sketch map that flows to before the separated space.
Fig. 7 is that the present invention separates blood in the soft bag and is full of the sketch map that flows to behind the separated space.
Fig. 8 is direction of rotation of the present invention when opposite with expansion direction, the three-dimensional stressing conditions sketch map of visible component such as blood cell in the separated space.
Fig. 9 is direction of rotation of the present invention when opposite with expansion direction, the plane stressing conditions sketch map of visible component such as blood cell in the separated space.
Figure 10 is direction of rotation of the present invention when identical with expansion direction, the three-dimensional stressing conditions sketch map of visible component such as blood cell in the separated space.
Figure 11 is direction of rotation of the present invention when identical with expansion direction, the plane stressing conditions sketch map of visible component such as blood cell in the separated space.
The specific embodiment
Come the method that blood continuous centrifugal of the present invention separates is described in further detail below in conjunction with accompanying drawing and specific embodiment, in the hope of understanding operation principle of the present invention and workflow more lucidly, but can not limit protection scope of the present invention with this.
The invention belongs to the method that a kind of blood continuous centrifugal separates, for convenience of explanation, be separated into example with the continuous centrifugal of blood plasma in the whole blood here and set forth.The principle of this method is to place one to have the separation container that separates lacuna blood, by this separation container of high speed rotating, thereby blood plasma in the blood and other visible components is separated.Certainly except erythrocyte and separating plasma, can also be used for the separation of other visible components of blood.Density differs and is erythrocyte and blood plasma to the maximum in the blood, erythrocytic density maximum wherein, and the density minimum of blood plasma, and also the blood plasma of single component and erythrocyte are the blood constituents of expense maximum in the medical science.Therefore, the blood plasma in the blood being carried out the continuous centrifugal separation is the most basic, the simplest component separating.
If realize isolating continuously and efficiently blood middle-high density composition and low-density composition, at first to carry out the novelty design to the centrifugalize container.The present invention has designed earlier one and has separated lacuna in separation container, the shape of this lacuna is the curved body that includes outer wall and inwall, the separation container that contains this curved body lacuna is called the curved body container, can reach the purpose of blood constituent high-efficiency and continuous centrifugalize by the application of this container.The outer wall curved surface of above-mentioned separation lacuna curve of projection on perpendicular to the plane of rotating shaft is as follows with polar coordinate representation:
Figure 797538DEST_PATH_IMAGE001
Wherein, polar limit O is the intersection point on rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise direction, r is any utmost point of some footpath on the curve, and R is the utmost point footpath of curve distal-most end, θ 1Be the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ 2Be curve and separating plasma factor critical radius F rThe polar angle of intersection point, separating plasma factor critical radius F rThe required minimum centrifugal radius of separating plasma in unit interval when determining for rotating speed, θ 3Be a polar angle, its value is θ 21.5~3.5 times, θ 4Polar angle for the curve distal-most end; In described separation lacuna, [θ 1, θ 2) be the blood plasma district, [θ 2, θ 3) be the chaotic region, [θ 3, θ 4] be the blood cell district, b 1Be the curve coefficients in blood plasma district, b 2Be the curve coefficients of chaotic region, b 3Curve coefficients for the blood cell district.Because the form of expression of drop shadow curve is a helix, so define key point wherein with utmost point footpath, polar angle.Curve coefficients refers to that the utmost point footpath of any point on the curve is with respect to the rate of change of polar angle.The general shape of above-mentioned curve as shown in Figure 1, Fig. 1 is the perspective view of separating the lacuna curved surface in the blood continuous centrifugal of the present invention method of separating.When extracting desired blood component out in the separation lacuna of curved body container, constantly replenish whole blood again, make and separate the lacuna inner capacities and reach a dynamic balance, and then realize the blood continuous centrifugal separation process in the object of the invention.
The structure of curved body container includes the separation lacuna of curved body form, and this Vessel Design is a disc-shaped structure, is referred to as separator disk.Cylinder 1 and 2 two parts of outer cylinder in this separator disk includes.Interior cylinder 1 removably is fixed in the inner chamber of outer cylinder 2, leaves certain space between the internal chamber wall of outer cylinder 2 and the lateral wall of interior cylinder, and this space is exactly the separation lacuna 3 on the separator disk, as shown in Figure 3.Receive the soft bag of separation of once property use separating the lacuna content, blood continuous centrifugal separating process is to carry out in separating soft bag.
The internal chamber wall of above-mentioned outer cylinder is near-end and the far-end that includes apart from centre rotational axis line different distance, is a curved surface between described near-end and far-end, and this curved surface is being expressed as follows with polar form perpendicular to the drop shadow curve on the plane of rotating shaft:
Figure 26525DEST_PATH_IMAGE001
Wherein, polar limit O is the intersection point on rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise direction, r is any utmost point of some footpath on the curve, and R is the utmost point footpath of curve distal-most end, θ 1Be the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ 2Be curve and separating plasma factor critical radius F rThe polar angle of intersection point, separating plasma factor critical radius F rThe required minimum centrifugal radius of separating plasma in unit interval when determining for rotating speed, θ 3Be a polar angle, its value is θ 21.5~3.5 times, θ 4Polar angle for the curve distal-most end; In described separation lacuna, [θ 1, θ 2) be the blood plasma district, [θ 2, θ 3) be the chaotic region, [θ 3, θ 4] be the blood cell district, b 1Be the curve coefficients in blood plasma district, b 2Be the curve coefficients of chaotic region, b 3Curve coefficients for the blood cell district.Above-mentioned curved surface is exactly as the specific implementation form of separating the lacuna lateral wall.
Above-mentioned interior cylinder 2 and outer cylinder 1 coaxial combination form integral body separator disk as shown in Figure 3.Generally speaking, the lateral wall of interior cylinder can be designed as with the internal chamber wall of outer cylinder has different curved surface forms or identical curved surface form, if the latter forms a thickness within it and separates lacuna 3 uniformly between cylinder lateral wall and the outer cylinder internal chamber wall.
Place one in the separation lacuna in described continuous curve surface separator disk and separate soft bag.When blood engorgement is separated soft bag, the lateral wall of cylinder in the medial wall of the soft bag of described separation can be close to, the internal chamber wall of outer cylinder can be close in the lateral wall of separating soft bag, namely in separating soft bag, form one with separate the separated space that lacuna is close, the continuous centrifugal separation process of blood is finished in this separated space.The purpose of separating soft bag is set in separator disk is repeatedly to use separator disk and disposable use to separate soft bag, avoiding the pollution between the different blood, and be convenient to improve separation efficiency and reduce separation costs.
The soft bag of described disposable separation is a kind of flat bag shaped structure that has a plurality of liquid transmission pipelines, its structure as shown in Figure 4, Fig. 4 is the deployed configuration sketch map that separates soft bag among the present invention.The soft bag of described separation is formed with three pipelines that are connected with bag by separating bag; Wherein, separating bag is rectangular software plastic bag, and it makes the standard that material will satisfy medical plastic; The seamed edge on the long limit of this separation bag is provided with three pipelines inside and outside the connection bag, lays respectively at front portion, middle part, the rear portion of separating soft bag; Wherein, the zone that is arranged in the front portion and stretches into the pipeline place of separating bag because the density of blood plasma is less, is in the position nearest apart from rotating shaft for separating the blood plasma district of lacuna in separating lacuna, so claim that this pipeline is blood plasma pipeline 4, be used for extracting isolated blood plasma from separating soft bag; The zone that is arranged in the middle part and stretches into the pipeline place of separating bag is used for carrying whole blood to the centrifugalize bag, so be called whole blood pipeline 5 for separating the chaotic region of lacuna; Be positioned at the pipeline connection at rear portion and extend into and separate bag, because the density of blood cell is bigger, in separating lacuna, be in apart from rotating shaft position farthest, the zone at this pipeline place is the blood cell district that separates in the lacuna, so claim that this pipeline is blood cell pipeline 6, be used for extracting isolated blood cell from separating soft bag.
When using continuous centrifugal separation method of the present invention, blood is that blood inlet enters into and separates soft bag from the flexible pipe that is arranged in the middle part of separating soft bag and connects, and the whole blood that contains various blood constituents is full whole separated space gradually.In full process, be subjected to the continuous action of centrifugal force, blood plasma separates gradually with visible components such as blood cells, in separated space from the low centrifugal force district of near-end to the high centrifugal force field of far-end, be rendered as the distribution of visible component segmentation and regionalizations such as blood plasma, whole blood and blood cell successively.Separated space middle part assembling major part not separating whole blood be called the chaotic region, the low centrifugal force zone of separated space near-end is called the blood plasma district, the high centrifugal force zone of separated space far-end is called the blood cell district.
Separator disk high speed rotating under the drive of flexible axle as the centrifugalize container, the structure of its whole centrifugal separation system and operation logic are at patent application 201020293871.4(differential centrifugal separation system) in illustrate, as shown in Figure 2, repeat no more herein.Separator disk need be around the rotating shaft high speed rotating that is positioned at the center under the drive of flexible axle, for the centrifugalize of blood provides power.Flexible axle stretches into and is connected and fixed on the rotating shaft position that is positioned at the separator disk center, the drive separation container rotates, described blood cell pipeline, whole blood pipeline and blood plasma pipeline are communicated with the tube for transfusion of design in the flexible axle, thereby require flexible axle to possess the dual-use function of power transmission and liquid transfer.
On the centrifugalize container, there is an intersection point central shaft of separator disk and separator disk bottom surface, and this intersection point is the junction point of flexible axle and separator disk, also are the power resources points that separator disk rotates.In the time of when the whole blood that contains the whole blood composition enters the separation bag from the whole blood pipeline in, the formation separated space because the lacuna of the interior lateral wall of this separation bag and separator disk fits can think that namely blood has entered in the separation lacuna of centrifugalize container.In the separated space of high speed rotating, owing to be subjected to the continuous action of centrifugal force, entering into the whole blood that separates bag from the whole blood pipeline can flow and pile up by higher separated space far-end along the medial wall of separating bag to centrifugal force, near-end to separated space develops gradually after the far-end of this separated space is full of, as shown in Figure 5, the whole blood that is oriented to of arrow 7 enters the flow direction that separates in the lacuna among the figure.
In the process that whole blood is piled up, the whole blood that is deposited in the separated space distal-most end is bigger owing to being subjected to action of centrifugal force, and the various compositions in the whole blood begin to separate.Continuous action along with centrifugal force, blood plasma strengthens gradually with visible components such as blood cells and separates, visible components such as blood cell are separated the blood inlet of back from the separated space far-end to the centre and are piled up, the blood plasma then blood inlet from the separated space near-end to the centre is piled up, when these two kinds of deposits meet near blood inlet, mixed interface can appear, simultaneously because the constantly whole blood shock effect of injecting, the single component that is separated herein mixes with whole blood, forms the chaotic region.The state of blood this moment in separating soft bag as shown in Figure 6, arrow 7 separates lacuna later on to the flow direction that separates the lacuna far-end among the figure for whole blood enters, 8 of arrows are after whole blood is accumulated to blood inlet, and the whole blood part that enters is to separating the moving direction of lacuna proximal flow.
When being full of visible components such as soft bag and blood plasma and blood cell when the input of whole blood and realizing separating, the blood plasma of Plasma Pheresis/Apheresis Plasma standard appears meeting in the separated space near-end, can extract visible components such as blood cell respectively out from separated space near-end extraction blood plasma with from the separated space far-end, inject whole blood at blood inlet simultaneously, the whole blood input quantity is the summation of blood cell and blood plasma extraction amount, thereby the cumulative volume of keeping liquid in the whole separated space is constant, realizes dynamic equilibrium.Blood and the various compositions states of this moment in separated space separated thereof are as shown in Figure 7, arrow 7 and arrow 8 are respectively whole blood and enter when separating soft bag to separating the direction that far and near two ends of lacuna flow among the figure, and 9 of arrows are the flow directions when being positioned at the blood plasma that separates the lacuna near-end and extracting out by blood plasma pipeline 4.
Method of the present invention is the optimization method that a kind of blood continuous centrifugal separates, and in the dynamic equilibrium of whole blood input and blood plasma and blood cell output procedure, the blood in the separated space has been subjected to many-sided effect power.One, blood itself is non-newtonian liquid, blood plasma in the blood and visible component have significant viscosity, the negative pressure that the malleation of whole blood input and blood plasma blood cell extract has impelled the whole blood in the separated space to flow, and the lateral wall of separated space, medial wall stop whole blood to flow to the mobile generation frictional force of whole blood, the closer to sidewall, flow velocity is more slow, produces the current gradient lamination; They are two years old, be subjected to the continuous action of centrifugal force, visible components such as the blood cell trend that density is bigger in the whole blood is distributed in far-end and the lateral wall of separated space, thereby is rendered as the segmentation distribution of visible components such as blood plasma, whole blood and blood cell in separated space successively to the high centrifugal force field of far-end by the low centrifugal force district of near-end; Its three, the influence of gravitate, the bottom that the visible components such as blood cell that the density in the separated space is bigger trend towards being deposited in separated space forms assembles line, makes these visible components more near the blood plasma mouth.
For this reason, when blood plasma is extracted out by the blood plasma pipeline fast in the blood plasma district, the blood plasma axial flow closes on visible components such as the blood cell generation drag force of separating interface to lateral wall and bottom, impel the component effect of the contrary centrifugal force of these visible components and spread along the blood plasma axial flow direction along the bottom of separated space, diffuse into the blood plasma mouth even and influence the blood plasma quality of collection.The principle of optimality of the present invention is shown in Fig. 8,9: when curved body container during at high speed rotating, separate lacuna outer wall 20 and produce two power by the visible components such as blood cell 19 in 21 pairs of separated spaces of soft bag sidewall, one is centripetal force 15, direction is pointed to limit, another is frictional force 13, and direction is the tangential direction of direction of rotation.When the expansion direction of the direction of rotation 10 of curved body container and described curve is opposite, drag force 14 action directions of 11 pairs of visible components such as blood cell of frictional force 13 directions and blood plasma axial flow are identical, visible components such as meeting acceleration blood cell spread to the blood plasma mouth, adding gravity 12 factors can cause blood cell to flow to the separated space bottom, make blood cell more be easy to sneak into plasma flow near the blood plasma mouth, and then have influence on the disintegrate-quality of blood plasma.Yet, as shown in Figure 10 and Figure 11, when the expansion direction of the direction of rotation 16 of curved body container and described curve is identical, drag force 14 action directions of 11 pairs of visible components such as blood cell 19 of frictional force 17 directions and blood plasma axial flow are opposite, and the visible components such as blood cell that can suppress to be deposited in because of gravity 12 separated space bottom spread to the blood plasma mouth.By control direction of rotation and not increasing under the situation of separation costs, can improve disintegrate-quality like this, therefore the expansion direction of the direction of rotation of described curved body container and described curve is identical has more optimization.
Embodiment 1
Constituted the separation lacuna by the continuous curve surface body on the centrifugalize container in the present embodiment, the lateral wall curved surface of this separation lacuna curve of projection on perpendicular to the plane of rotating shaft is:
Figure 824717DEST_PATH_IMAGE002
Wherein, polar limit O is the intersection point on rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, and the positive direction of polar angle is clockwise direction, and r is any utmost point of some footpath on the curve, R=199mm, θ 1=0 degree, θ 2=90 degree, θ 3=255 degree, θ 4=380 degree; In described separation lacuna, [0 o, 90 o) be the blood plasma district, [90 o, 255 o) be the chaotic region, [255 o, 380 o] be the blood cell district, b 1=0.8, b 2=0.4, b 3=0.2.The direction of rotation of this separation container is identical with the expansion direction that separates lacuna.
After whole blood is full of whole separation lacuna, whole blood continues input from blood inlet, and blood plasma continues visible components such as extraction and blood cell and continues to extract out from the blood cell mouth from the blood plasma mouth, and input quantity equates with discharge, make and separate total liquid volume maintenance balance in the lacuna, thereby reach the purpose of continuous separating blood.
Undoubtedly, the structure that the present invention enumerates in above-described embodiment the method that the blood continuous centrifugal separates form and curve form, can be formed and curve form by other similar structures in addition.Generally speaking, protection scope of the present invention also comprises other conversion that it will be apparent to those skilled in the art that and substitutes.

Claims (4)

1. the blood optimization method that continuous centrifugal separates in the curved body container, it is characterized in that, be provided with the separation lacuna in the described curved body container, this curved body container is around the rotating shaft high speed rotating, and the blood that utilizes centrifugal force will be injected in the described separation lacuna goes out heterogeneity by the density size separation; Described separation lacuna is the curved body that includes outer wall and inwall, and this outer wall curved surface that separates lacuna curve of projection on perpendicular to the plane of rotating shaft is as follows with polar coordinate representation:
Figure 2012100035800100001DEST_PATH_IMAGE001
Wherein, polar limit O is the intersection point on rotating shaft and described plane, and polar pole axis L is the ray that limit arrives curve initiating terminal direction, the positive direction of polar angle is clockwise direction, r is any utmost point of some footpath on the curve, and R is the utmost point footpath of curve distal-most end, θ 1Be the polar angle of curve initiating terminal, its value is 0 degree of this polar coordinate system, θ 2Be curve and separating plasma factor critical radius F rThe polar angle of intersection point, separating plasma factor critical radius F rThe required minimum centrifugal radius of separating plasma in unit interval when determining for rotating speed, θ 3Be a polar angle, its value is θ 21.5~3.5 times, θ 4Polar angle for the curve distal-most end; In described separation lacuna, [θ 1, θ 2) be the blood plasma district, [θ 2, θ 3) be the chaotic region, [θ 3, θ 4] be the blood cell district, b 1Be the curve coefficients in blood plasma district, b 2Be the curve coefficients of chaotic region, b 3Curve coefficients for the blood cell district; The direction of rotation of described separation container is identical with the expansion direction that separates lacuna.
2. a kind of blood according to claim 1 optimization method that continuous centrifugal separates in the curved body container is characterized in that, described curve coefficients refers to that the utmost point footpath of any point on the curve is with respect to the rate of change of polar angle.
3. the optimization method that separates of a kind of blood according to claim 1 continuous centrifugal in the curved body container, it is characterized in that, the expansion direction of described separation lacuna refers to: in the blood plasma district of described curve, any 2 p and q are arranged, its polar angle is respectively θ p and θ q, the corresponding utmost point directly is respectively rp and rq, and θ 1≤ θ p<θ q≤θ 2, while rp<rq, some p is described curve expansion direction along the direction that curvilinear path moves to a q.
4. a kind of blood according to claim 1 optimization method that continuous centrifugal separates in the curved body container is characterized in that the separation process of described blood continuous centrifugal specifically comprises the steps:
The first step, the described curved body container of high speed rotating places the soft bag of the separation that separates in lacuna high speed rotating thereupon, and front portion, middle part, the rear portion of the soft bag of this separation is respectively equipped with a flexible pipe of carrying liquid;
Second step, blood is injected into the soft bag of separation by being positioned at the flexible pipe that separates soft bag middle part, after treating that whole blood is full of the soft bag of separation space, separate soft bag and separate lacuna and fit tightly formation and separate the inside separated space that lacuna is close, blood is subjected under the centrifugal force continuous action various compositions segmentation and regionalization gradually in separating soft bag, blood plasma accumulates in separation soft bag front portion gradually and forms a blood plasma district, comprise blood cell and accumulate in separation soft bag rear portion gradually and form a blood cell district in interior visible component, and separate the middle part of soft bag because the lasting input of whole blood forms a chaotic region;
The 3rd step, separate the anterior flexible pipe of soft bag at blood plasma district extraction blood plasma from being arranged on, extraction comprises blood cell in interior visible component in the blood cell district from being arranged on the flexible pipe that separates soft bag rear portion, continue the input whole blood from being arranged on the flexible pipe that separates soft bag middle part simultaneously, reach the dynamic equilibrium that separates soft bag inner capacities by the control input with extracting out, and then realize that the continuous centrifugal of blood separates.
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CN101172207A (en) * 2007-10-12 2008-05-07 经建中 Separator disk on multi-cell component mix liquid separating system and application method of the same

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US4419089A (en) * 1977-07-19 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Blood cell separator
US4387848A (en) * 1977-10-03 1983-06-14 International Business Machines Corporation Centrifuge assembly
JPH06154308A (en) * 1992-08-14 1994-06-03 Fresenius Ag Method and apparatus for continuous adjustment of cell suspension
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