CN103299194A - Blood matching unit and combiner for disposable blood transfusion and cross matching experiment - Google Patents
Blood matching unit and combiner for disposable blood transfusion and cross matching experiment Download PDFInfo
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- CN103299194A CN103299194A CN2011800434378A CN201180043437A CN103299194A CN 103299194 A CN103299194 A CN 103299194A CN 2011800434378 A CN2011800434378 A CN 2011800434378A CN 201180043437 A CN201180043437 A CN 201180043437A CN 103299194 A CN103299194 A CN 103299194A
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- blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2205/00—General characteristics of the apparatus
- A61M2205/60—General characteristics of the apparatus with identification means
- A61M2205/6009—General characteristics of the apparatus with identification means for matching patient with his treatment, e.g. to improve transfusion security
Abstract
A blood matching unit(1) and a combiner for disposable blood transfusion and cross matching experiment are provided, the blood matching unit(1) comprises a sealed transparent experiment tube (11)and two sample application tubes(12,13), and the two sample application tubes(12,13) communicate with the experiment tube(11). The combiner comprises N2 blood matching units(1), N is the number of blood matched people, N=2, the serum sample application tubes(12)and the hematocyte application tubes(13)on the all blood matching units(1) are matched circularly according to the blood cross matching experiment principle, so that the all serum sample application tubes(12) correspond to the serums of blood matched people respectively, and the all hematocyte sample application tubes(13) correspond to the hematocytes of blood matched people respectively, and the serum sample application tubes(12) corresponding to the same serums are combined together to form N serum sample application units, the hematocyte sample application tubes(13) corresponding to the same hematocytes are combined together to form N hematocyte sample application units. In this way, the blood cross matching experiment will be convenient, quick, handled simplely and regularly, and less prone to error.
Description
Blood mathcing test unit and combiner technical field for cross match blood test of disposably transfusing blood
The present invention relates to a kind of medical test equipment, and in particular to a kind of to be used for the disposably blood mathcing test unit of blood transfusion cross match blood test and combine the combiner to be formed using the unit.Background technology
Up to the present, the situation of all-level hospitals Blood Transfusion Dept. blood transfusion cross match blood test to be done is the principle according to safe transfusion, apply for the blood source (as A type RH positive patients need to transfuse blood, the positive blood donors of A types RH must be sought) of abo blood group and RH blood group phase homotypes, the blood cell and serum of blood donor and receptor are separated respectively and blood cell 50% saline suspension is made into and serum is dispensed in test tube with standby.Process of the test, which is mainly, allows the serum and blood cell of blood donor and receptor intersect allowing it in vitro to react to observe its haemolysis agglutination phenomenon again, and can judgement with high safety transfuse blood.
In the prior art, blood transfusion cross match blood test also there is no special implement, typically be tested using common test tube, this to test a such as patient only defeated person-portion blood, then when transfusing blood cross match blood test, two test tubes of primary and secondary should be taken to be separately added into：Receptor's serum+blood donor's blood cell（Supervisor) and receptor's blood cell+blood donor's serum（Secondary pipe）.25-30 °C, centrifugation in 5-10 minutes is taken out, haemolysis and agglutination phenomenon are whether there is using visually observing, agglutination phenomenon will be whether there is with micro- sem observation on blood instillation slide after fork again, final report (FR) primary and secondary pipe whether there is haemolysis and agglutination phenomenon, conclusion " being consistent " or " not being consistent ", and judge that with this blood of this blood donor could be inputted.
If in the case that receptor needs massive transfusion (more than two person-portions) donor blood, its cross match blood test done before transfusing blood is just increasingly complex.Such as y-bend one is accomplished by six test tubes (because cross match blood test will be carried out between every blood donor and receptor and between every blood donor), if receptor needs trident for the moment, that wants twelve earthly branches test tube, repeated multiple times blood cell and serum by receptor and three blood donors is carried out from numbering to being loaded (to be only loaded this task operator every position sample-adding with sample injector and heal ball and serum respectively once to every test tube, then twelve earthly branches test tube will be loaded 24 times, but also blood cell and serum are added to supervisor and time pipe in the way of dislocation is matched, it can be seen that its operation is extremely complex cumbersome), therefore in the case of needing a large amount of blood of multidigit blood donor, cross matching process is increasingly complex cumbersome and error-prone.
The content of the invention
The present invention is for extremely complex cumbersome using the operation present in common test tube progress blood transfusion cross match blood test in the prior art, and the problem of easily malfunction, and a kind of blood mathcing test unit available for disposable blood transfusion cross match blood test is provided, based on the unit, the present invention also provides a kind of blood mathcing test combiner for cross match blood test of disposably transfusing blood.The operating procedure of disposable blood transfusion cross match blood test can be greatly simplified using the present invention, and it is not error-prone.
In order to achieve the above object, the present invention is adopted the following technical scheme that：
Blood mathcing test unit for cross match blood test of disposably transfusing blood, including transparent developmental tube, the blood mathcing test unit also includes two sample-adding pipes for being respectively used to increase serum and blood cell, and the transparent developmental tube is a seal cavity, and two sample-addings pipe is connected conducting with developmental tube.
In the optimal example of such scheme, the blood mathcing test unit also includes the observation portion for micro- sem observation.
Further, the observation portion is the transparent developmental tube of flat.' further, the observation portion is transparent experiment bottom of the tube, and the transparent experiment bottom of the tube is flat.
Further, the observation portion is sealed thin transparent membrane vesicle, and the thin transparent membrane vesicle is connected conducting with developmental tube.
Further, the thin transparent membrane vesicle is oblate shape, is molded with the ^ ^ of developmental tube one.Further, the junction of the thin transparent membrane vesicle and developmental tube is away from lcm at the top of developmental tube.Further, two sample-addings pipe connects " Y " type that to be formed, and the public end in its bottom connects conducting with developmental tube.
Further, the sample-adding pipe is integrally formed with developmental tube.
Further, the sample-adding pipe is docked by spiro connection structure with developmental tube or docked by seccotine.Further, the shape and/or color of two sample-addings pipe are different, correspondence serum sample-adding and blood cell sample-adding.
Further, the section of two sample-addings Guan Zhongyi sample-adding pipes is circle, and the section of another sample-adding pipe is square.
Further, the entrance point of two sample-addings pipe sets structure of having any different.
Further, the difference structure is that the entrance point of a sample-adding pipe is spherical or cylindric, and the entrance point of another sample-adding pipe is column or cube shape.
Further, the developmental tube is made up of polyethylene transparent plastic, its a diameter of 1.2cm, long 4cm.
Based on such scheme, provided by the present invention for the blood mathcing test combiner of disposable blood transfusion cross match blood test, the combiner includes N2Individual above-mentioned blood mathcing test unit, N is match person's number, N>2, circulation pairing is carried out to the serum sample-adding pipe on all blood mathcing test units and blood cell sample-adding pipe according to cross match blood test principle, so that all serum sample-adding pipes correspond to the serum of corresponding match person respectively, all blood cell sample-adding pipes correspond to the blood cell of corresponding match person respectively, and combine the serum sample-adding pipe of the identical serum of correspondence, N number of serum sample-adding unit is formed, the blood cell sample-adding pipe of the identical blood cell of correspondence is combined, N number of blood cell sample-adding unit is formed.
Further, during the circulation pairing, by N2Individual blood mathcing test cell-average is divided into N number of blood mathcing test unit group, each blood mathcing test unit group includes N number of blood mathcing test unit, serum sample-adding pipe in wherein each blood mathcing test unit group on N number of blood mathcing test unit is grouped together into N number of serum sample-adding unit, while the blood cell sample-adding pipe on each blood mathcing test unit in each blood mathcing test unit group is grouped together into N number of blood cell sample-adding unit with the blood cell sample-adding pipe on a certain blood mathcing test unit of other all blood mathcing test unit groups respectively.
Further, during the circulation pairing, by N2Individual blood mathcing test cell-average is divided into N number of blood mathcing test unit group, each blood mathcing test unit group includes N number of blood mathcing test unit, blood cell sample-adding pipe in wherein each blood mathcing test unit group on N number of blood mathcing test unit is grouped together into N number of blood cell sample-adding unit, while the serum sample-adding pipe on each blood mathcing test unit in each blood mathcing test unit group is grouped together into N number of serum plus auspicious unit with the serum sample-adding pipe on a certain blood mathcing test unit of other all blood mathcing test unit groups respectively.
Further, as N=2, the serum sample-adding pipe and blood cell sample-adding pipe that each blood mathcing test unit group is included on 1 blood mathcing test unit, two blood mathcing test units are respectively combined to form serum sample-adding unit and blood cell sample-adding unit.
Further, the blood mathcing test combiner also includes a test stand for being used to fix blood mathcing test unit, the test stand includes a plurality of fixed plates and a fixed mount, offered in the fixed plate with the fixing hole that developmental tube is engaged in blood mathcing test unit, the week determines to be provided with frame is engaged slot with fixed plate.
Further, the slot arrays distribution.
Further, a plurality of breach are radially offered on the fixing hole of the fixed plate, will
Fixing hole is split to form a plurality of fixing tooths along side.
Further, the bottom of the breach is circular hole.
Further, the breach is symmetrical.
Further, the fixed plate and fixed mount are made up of plastics, lucite or metal material.The reaction principle of cross matching according to necessary to the present invention that such scheme is obtained complies fully with safe transfusion, it abandons the original process that the cumbersome of cross match blood test is made of test tube and is easily malfunctioned, cross reaction is participated in the plastics reaction tube that pre-set sample-adding unit reaches needed for flowing downwardly in the blood mathcing test combiner for the disposable blood transfusion cross match blood test that the serum of experiment and blood cell are provided by the present invention, without carrying out sample-adding repeatedly between different test tubes；After completion of the reaction, first visually observe again to press to reactant in the thin transparent membrane vesicle for microscope inspection Check reserved in reaction tube and carry out microscopy respectively so that experiment is convenient, fast, simple to operate regular and not error-prone.
In structure, simple in construction, low manufacture cost of the invention is easy to industrial production to make.Brief description of the drawings
The present invention is further illustrated below in conjunction with the drawings and specific embodiments.
Fig. 1 is the structural representation of blood mathcing test unit in the present invention.
Fig. 2 is the structural representation of Y type blood mathcing test units.
Fig. 3 is the side view of the blood mathcing test unit of flat.
Fig. 4 is the side view of the blood mathcing test unit of bottom flat shape.
Fig. 5 is the blood mathcing test cellular construction schematic diagram with thin transparent membrane vesicle.
Fig. 6 is an one blood mathcing test combiner of fork.
Fig. 7 is a two blood mathcing test combiners of fork.
Fig. 8 is the sectional view of test stand in combiner.
Fig. 9 be test stand in fixed plate structural representation.
Figure 10 is test stand top view.
Figure 11 is blood donor's cross matching schematic diagram of Washed Red Blood Cells.Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, with reference to being specifically illustrating, the present invention is expanded on further.
Referring to Fig. 1, the present invention is provided to the blood mathcing test unit 1 of disposable blood transfusion cross match blood test, the test unit can be used for substituting the teat glass used in disposable blood transfusion cross match blood test.
Special hygienic requirements based on blood mathcing test utensil and the requirement for being easy to viewing test, the blood mathcing test unit 1 is overall can be all made of polyethylene transparent plastic, but be not restricted to that this, as long as above-mentioned requirements can be reached.
As seen from the figure, the blood mathcing test unit 1 mainly includes transparent developmental tube 11, two sample-adding pipes 12,13 two parts.
Wherein transparent developmental tube 11 is whole cross match blood test reacting environment, and it is seal cavity.Developmental tube is made up of polyethylene transparent plastic, its a diameter of 1.2cm, long 4cm.
Two sample-adding pipes 12,13 are respectively used to serum and blood cell that conduction carries out cross match blood test, such two sample-addings pipe 12,13 is so easy to corresponding serum and blood cell importeding into progress cross matching reaction in transparent developmental tube 11 respectively with transparent developmental tube 11 up to conducting is connect.
Two sample-adding pipes 12,13 length can with identical or different, as needed depending on.In the utility model, from the consistent lucite pipe of two length as sample-adding pipe 12,13, its length is 18cm, and the inner diameter of pipe is 5mm,, can be according to pipe of the actual demand using adaptable length and width but the present invention is not limited thereto.
Because two sample-adding pipes 12,13 are respectively used to sample-adding serum and blood cell, i.e., respectively serum sample-adding pipe 12 and blood cell are loaded pipe 13.For the ease of distinguishing the sample-adding pipe of two difference in functionalitys, the present invention use kinds of schemes distinguish and determine one for serum plus auspicious pipe 12 and another be that blood cell is loaded pipe 13.
For this, the present invention distinguishes serum sample-adding pipe 12 and blood cell sample-adding pipe 13 using tubulose of different shapes, section is such as used to be used as blood cell sample-adding pipe 13 for the pipe of toroidal, section is used to be used as serum sample-adding pipe 12 for the pipe of square shape simultaneously, but the present invention is not limited thereto, serum sample-adding pipe 12 and blood cell sample-adding pipe 13 can also be distinguished and determined using other pipes of different shapes.
Alternatively, the present invention can also be used sets structure of having any different in two entrance points for being loaded pipe 12,13.The plastic tube of above-mentioned specification is used as the body of two sample-adding pipes 12,13 is identical, and the entrance point of blood cell sample-adding pipe 13 is designed to that section is the spherical of " 0 " type, while the entrance point that serum is loaded into pipe 12 is designed to that section is " mouth " cube shape（As shown in Figure 1）.Serum sample-adding pipe 12 and blood cell sample-adding pipe 13 are distinguished from there through entrance point of different shapes, but specific shape is not limited to this, can equally use other shapes, as long as can reach that differentiation purpose just can be with such as spherical and column.
As the third scheme, serum sample-adding pipe 12 and blood cell sample-adding pipe 13 can be distinguished and determined using the pipe of different colours, this scheme is common technology, is not described in detail herein.The program also has a variety of derivative schemes, for example carry out distinguishing using different labels etc., it is equally applicable.The program can be applied in above two scheme simultaneously.
Based on such scheme, in the use of the present invention, related personnel can be very directly perceived and accurately determines which is managed and got rid of for increase serum, which pipe in healing ball, it is very convenient and not error-prone.
Pipe 12 and blood cell sample-adding pipe are loaded for serum:13 can have a variety of with the connected mode of transparent developmental tube 11, and serum sample-adding pipe 12 and blood cell sample-adding pipe 13 directly connect conducting with transparent developmental tube 11 respectively.It can be such as made of one piece with transparent developmental tube 11, corresponding connecting hole can also be set on transparent developmental tube 11, and serum sample-adding pipe 12 and blood cell sample-adding pipe 13 carry out docking one entirety of formation respectively by spiro connection structure or by seccotine and transparent developmental tube 11.
Referring to Fig. 2, " Y " type is integrally formed between serum sample-adding pipe 12 and blood cell sample-adding pipe 13, the public end in its bottom connects conducting with developmental tube.The mode of connection can be to be made of one piece with transparent developmental tube 11, corresponding connecting hole can also be set on transparent developmental tube 11, the serum sample-adding pipe 12 of " Y " type and the public end in bottom of blood cell sample-adding pipe 13 carry out docking one entirety of formation by spiro connection structure or by seccotine and transparent developmental tube 11.
Referring to Fig. 3 to 5, the blood mathcing test unit 1 that the present invention is provided also includes an observation portion 14, and the observation portion 14 is used for whetheing there is the adverse reactions such as tiny aggegation after cross reaction in the micro- sem observation transparent developmental tube 11.
As shown in figure 3, the observation portion 14, can be the body of whole transparent developmental tube.For ease of observation, the body of transparent developmental tube is flat.
As shown in figure 4, the observation portion 14 is the bottom of transparent developmental tube.For ease of observation, the bottom of transparent developmental tube is flat.
As shown in figure 5, observation portion 14 is a thin transparent membrane vesicle 14, the thin transparent membrane vesicle 14 is the cavity of sealing shape, and it is connected conducting with developmental tube 11.
For the ease of observation, thin transparent membrane vesicle 14 is designed as oblate shape, is made of one piece with developmental tube by the present invention.Its specific size is close with unitary coin, i.e. diameter about 2-4cm, but be not restricted to that this.Meanwhile, the junction of thin transparent membrane vesicle 14 and developmental tube 11 is away from lcm at the top of developmental tube.Reaction result is not influenceed so, also allows for observation.Thus, directly it can be observed by the present invention of thin transparent membrane vesicle 14 by microscope, without the tedious steps observed by teat glass to slide, i.e. side
Just problem present in operating process of the teat glass to slide is avoided again.
The blood mathcing test unit 1 obtained based on such scheme when in use, can stick the label of different colours in the neck of developmental tube 11, get rid of in writing cross unit number, corresponding blood cell and serum numbering, medical information（Such as department, bed label, name, bar code information）Deng.
In order to quickly and accurately enter fi blood mathcing tests to match person, the present invention also provides a kind of blood mathcing test combiner for cross match blood test of disposably transfusing blood.
Referring to Fig. 6 and Fig. 7, the combiner includes a plurality of above-mentioned blood mathcing test units, and the number of blood mathcing test unit is corresponding with match number N, is N2, N>2 (because the number with scholar is at least the receptor of 2 two, i.e., one and a blood donor）.According to cross match blood test principle, the serum of every match person will carry out cross reaction experiment with the blood cell of other match persons, its blood cell will carry out cross reaction experiment with the serum of other match persons simultaneously, circulation pairing is carried out to the serum sample-adding pipe on all blood mathcing test units and blood cell sample-adding pipe according to cross match blood test principle for this, so that all serum sample-adding pipes correspond to the serum of corresponding match person respectively, all blood cell sample-adding pipes correspond to the blood cell of corresponding match person respectively, and combine the serum sample-adding pipe of the identical serum of correspondence, form N number of serum sample-adding unit, the blood cell sample-adding pipe of the identical blood cell of correspondence is combined, form N number of blood cell sample-adding unit.
Based on such scheme, the present invention provides a kind of specific circulation matching method（As shown in Figure 7）, it is by N2Individual blood mathcing test cell-average is divided into N number of blood mathcing test unit group, each blood mathcing test unit group includes N number of blood mathcing test unit, serum sample-adding pipe in wherein each blood mathcing test unit group on N number of blood mathcing test unit is grouped together into N number of serum sample-adding unit, while the blood cell sample-adding pipe on each blood mathcing test unit in each blood mathcing test unit group is grouped together into N number of blood cell sample-adding unit with the blood cell sample-adding pipe on a certain blood mathcing test unit of other all blood mathcing test unit groups respectively.
The combiner so formed, wherein often assemble one serum sample-adding unit of N number of blood mathcing test unit correspondence in blood test unit group, N number of blood mathcing test unit corresponds to N number of blood cell sample-adding unit respectively, so in blood test unit group is often assembled, it is to add the serum and blood cell of itself to have a serum sample-adding unit, it is used to contrast, unit as a comparison, so it is easy to observation to compare, the cross matching that remaining N-1 blood mathcing test unit is used to complete with other match person's blood cells is reacted.
Its is easy to operate, is loaded as long as the serum of N match persons is loaded into unit by N number of serum respectively, i.e. the serum of the corresponding match person of each serum sample-adding unit, and N number of serum therein adds pipe all to add the serum of the match person；The blood cell of N match persons is passed through into N number of blood cell respectively simultaneously
Sample-adding unit is loaded, i.e., the blood cell of each blood cell sample-adding unit one match person of correspondence, therein
N number of blood cell adds pipe all to add the blood cell of the match person.Thus, in assembling blood test unit group one, you can the serum of a match person and the cross match blood test of other all match person's blood cells are completed, while having the reaction comparison unit of an autoserum and blood cell.
Based on the principle of above-mentioned concrete scheme, the present invention provides another circulation matching method, and the matching method is that the combined method that above-mentioned serum sample-adding pipe and blood cell are loaded into pipe is exchanged, i.e., by N2Individual blood mathcing test cell-average is divided into N number of blood mathcing test unit group, each blood mathcing test unit group includes N number of blood mathcing test unit, blood cell sample-adding pipe in wherein each blood mathcing test unit group on N number of blood mathcing test unit is grouped together into N number of blood cell sample-adding unit, while the serum sample-adding pipe on each blood mathcing test unit in each blood mathcing test unit group is grouped together into N number of serum sample-adding unit with the serum sample-adding pipe on a certain blood mathcing test unit of other all blood mathcing test unit groups respectively.
Its specific principle and mode of operation are similar to above, are not repeated here herein.
When only two match persons, i.e. during N=2, now match cross matching is fairly simple, as long as using the serum of itself and the serum test of the blood cell of other side and the blood cell of itself and other side, without what control unit.Therefore, when the present invention is for N=2, there is provided a kind of combiner of optimization.Such as Fig. 6, the serum sample-adding pipe 12,22 and blood cell sample-adding pipe 13,23 that the combiner is included on two blood mathcing test units 1,2, two blood mathcing test units 1,2 are respectively combined to form serum sample-adding unit 3 and blood cell sample-adding unit 4.
Due to such scheme understand, when match number increase, the number of the blood mathcing test unit needed for it is multiplied, and it is many that its number compares, by it is hand-held be unpractical.Therefore, the present invention also provides a kind of test stand 100 for being used to fix blood mathcing test unit.
Referring to Fig. 8, the test stand 100 includes a plurality of fixed mounts 102 of fixed plate 101 and one.
Referring to Fig. 9, each fixed plate 101 is square, and the fixing hole 101a being engaged with developmental tube in blood mathcing test unit is offered thereon, is so easy to blood mathcing test unit to fix wherein.
Further, fixed plate 101 radially offers a plurality of breach 101b on fixing hole 101a, and fixing hole is split along side to form a plurality of fixing tooth 101c.
As illustrated, the present invention is symmetrical on fixing hole 101a to offer 5 breach 101b, the bottom of the breach is circular hole, and fixing hole is split to form 5 fixing tooth 101c along side, is so further improved to blood mathcing test unit fixed effect.
For the frame structure of fixed mount 102, generally a square frame, the left and right sides is support frame 102a,
Front and rear sides opening, open top simultaneously, and it is arranged side by side be provided with multiple slot 102b, and the spacing between slot 102b is engaged with fixed plate 101 so that simultaneously array distribution during fixed plate 101 can be inserted between slot 102b from the front and rear sides of fixed mount 102（As shown in Figure 10).All blood mathcing test units in whole combiner are so enabled to be placed in test stand 100, and the arrangement that can be carried out according to blood mathcing test unit group a group by a group is disposed, avoid experiment ^ member confusions from comparing to observation and bring unnecessary trouble, be so not only easy to observe but also can improve the convenience of cross match blood test.- for the material of test stand 100, fixed plate and fixed mount thereon can be made up of plastics, organic glass or metal material.
The specific cross match blood test process of the blood mathcing test combiner obtained based on such scheme is as follows：
1st, a fork one is tested
Situation of the experiment for a receptor and a blood donor, as shown in Figure 6, the experiment is using an one blood mathcing test combiner of fork, the serum sample-adding pipe 12,22 and blood cell sample-adding pipe 13,23 that the combiner is included on two blood mathcing test units 1,2, two blood mathcing test units 1,2 are respectively combined to form serum sample-adding unit 3 and blood cell sample-adding unit 4.
The section of serum sample-adding pipe on wherein each blood mathcing test unit is ", type, the section of blood cell sample-adding pipe is " 0 " type.
First as needed by two blood mathcing test units 1,2 cross arrangements on test stand, while serum sample-adding pipe 12,22 and blood cell sample-adding pipe 13,23 are respectively combined to form serum sample-adding unit 3 and blood cell sample-adding unit 4.And serum is loaded in unit 3 and sticks green-ticket numbering on two " " type serum sample-adding pipes 12,22, while blood cell to be loaded in unit 4 and sticks red-ticket numbering on two " 0 " type blood cells sample-adding pipes 13,23.
During experiment sample-adding, 0.5ml serum is added by number to " " type patch green-ticket pipe, blood cell suspension is added by number to " 0 " type patch red-ticket pipe.
Wherein, it regard the blood mathcing test unit 1 for adding receptor's serum stoste and blood donor's blood cell as supervisor, the serum sample-adding addition receptor serum 0.5ml of pipe 12 thereon, while blood cell sample-adding pipe 13 thereon adds 50% blood donor's blood cell salt solution 0.3ml.
It regard the blood mathcing test unit 2 for adding receptor's blood cell and blood donor's serum as time pipe, the serum sample-adding addition blood donor serum 0.5ml of pipe 22 thereon, while blood cell sample-adding pipe 23 thereon adds 50% receptor's blood cell salt solution 0.3ml.
After sample-adding is completed, two blood mathcing test units are once operated：
(1) the intersection thing in above-mentioned two blood mathcing test unit in cuvette is gently shaken, is put into 25 °C of incubators and reacts 5-10 minutes after mixing；
(2) take out low-speed centrifugal 2 minutes, supernatant is being observed respectively, haemolysis, agglutination phenomenon is whether there is；
(3) if without haemolysis, agglutination phenomenon, touching in thin transparent membrane vesicle thereon to whether there is tiny agglutination phenomenon with micro- sem observation after reactant in cuvette is shaken up.
(4) when receptor's serum+blood donor's blood cell, blood donor's serum+receptor's blood cell are without haemolysis, agglutination phenomenon, clinic can transfuse blood after conclusion " being consistent " report.
2nd, y-bend one is tested
The experiment, for the situation of a receptor and two blood donors, is 0 for ease of stating receptor's numbering, it is 1,2 that two blood donors number respectively.
As shown in fig. 7, the experiment uses the blood mathcing test combiner of y-bend one.The combiner 9 (N2, the individual blood mathcing test unit 1-9 of N=3).According to cross match blood test principle, 9 blood mathcing test units are uniformly divided into three blood mathcing test unit groups A0, Al, A2, each blood mathcing test unit group includes three blood mathcing test units respectively.
Three " mouth " type serum sample-adding pipe 12,22,32 in blood mathcing test unit group AO on three blood mathcing test units 1,2,3 is wherein grouped together into serum sample-adding unit A01, serum sample-adding unit A01 corresponds to the serum of No. 0 receptor, i.e., serum sample-adding pipe 12,22,32 therein all adds the serum of No. 0 receptor.
Three " " plough serum sample-adding pipe 42,52,62 in blood mathcing test unit group A1 on three blood mathcing test units 4,5,6 is grouped together into serum sample-adding unit Al l, serum sample-adding unit Al l correspond to the serum of No. 1 blood donor, i.e., serum sample-adding pipe 42,52,62 therein all adds the serum of No. 1 blood donor. '
Three " " type serum sample-adding pipe 72,82,92 in blood mathcing test unit group A2 on three blood mathcing test units 7,8,9 is grouped together into serum sample-adding unit Α 2, serum sample-adding unit A21 corresponds to the serum of No. 2 blood donors, i.e., serum sample-adding pipe 42,52,62 therein all adds the serum of No. 2 blood donors.
Simultaneously, " O " type blood cell sample-adding pipe 13 in blood mathcing test unit 1 in blood mathcing test unit group AO is grouped together into blood cell sample-adding unit A22 respectively at " 0 " type blood cell sample-adding pipe 73 in blood mathcing test unit in " 0 " the type blood cell sample-adding pipe 43 and blood mathcing test unit group A2 in blood mathcing test unit 4 in blood mathcing test unit group A1, blood cell sample-adding unit A22 corresponds to the blood of No. 2 blood donors
Ball, i.e., blood cell sample-adding pipe 13,43,73 therein all adds the blood cell of No. 2 blood donors.
By " 0 " type blood cell sample-adding pipe 23 in blood mathcing test unit 2 in blood mathcing test unit group AO respectively in blood mathcing test unit 5 in blood mathcing test unit group A1 ' " type blood cell sample-adding pipe 83 is grouped together into blood cell sample-adding unit A12 to " type blood cell is loaded in pipe 53 and blood mathcing test unit group A2 in blood mathcing test unit 8 " 0; blood cell sample-adding unit A12 corresponds to the blood cell of No. 1 blood donor, i.e., blood cell sample-adding pipe 23,53,83 therein all adds the blood cell of No. 1 blood donor.
By in blood mathcing test unit 3 in blood mathcing test unit group AO ' " type blood cell is loaded pipe 33 respectively in blood mathcing test unit 6 in blood mathcing test unit group A1 " 0 " type blood cell is loaded in pipe 63 and blood mathcing test unit group A2 in blood mathcing test unit 9 " O " types blood cell sample-adding pipe 93 is grouped together into blood cell sample-adding unit A02; blood cell sample-adding unit A02 corresponds to the blood cell of No. 0 receptor, i.e., blood cell sample-adding pipe 33,63,93 therein all adds the blood cell of No. 0 receptor.
Pass through combinations thereof device, specification simplifies sample-adding program, combinations thereof device is fixed on test stand according to corresponding arrangement is grouped accordingly when in use, according to above-mentioned corresponding relation, corresponding blood cell suspension 0.3ml is added to each blood cell sample-adding unit, corresponding serum 0.5ml is added to each serum sample-adding unit.
Thus, the serum of a match person and the reaction test of all match person's blood cells can be completed in blood test unit group by being assembled one, including the match person itself blood cell and the control unit of serum, such as the blood mathcing test unit 3 in AO groups, blood mathcing test unit 7 in blood mathcing test unit 5 in A1 groups, A2 groups.
Ibid a fork one is tested for follow-up operation and report, is not repeated here herein.
Tested based on above-mentioned y-bend one, the combiner provided using the present invention can carry out the fork of trident one, four one etc. by that analogy according to the number of match person, only need Chinese pennisetum to realize corresponding packet according to such scheme and combine.Specific operation ibid, is not repeated here herein.
3rd, blood donor's cross matching of Washed Red Blood Cells is only needed（Portion's blood supply is clear）
For also having to progress cross matching only for receptor's Washed Red Blood Cells, its cross-over experiment pipe is identical with the cross matching pipe for whole blood, i.e., the blood mathcing test unit provided using the present invention.
The red cell suspension and receptor's serum of every blood donor are carried out single tube one by one（Supervisor）Intersection, it is specific as shown in figure 11. '
Script is applied to that sample-adding is numbered up to sample-adding pipe permutation and combination device in advance by the disposable blood transfusion cross matching experiment combiner that the present invention is provided from numbering, the complicated cumbersome match cross reaction process of sample-adding,
Accomplish that experiment label is eye-catching, by be loaded in coordinate set reached to prevent error it is rapid accurate, improve the degree of accuracy, the shortening time plays believable effect, particularly defeated multidigit blood when, matched using combinatorial coordinates, hence it is evident that reduce sample-adding program repeat number of times, such as trident one, as long as multi-fork one all concentrates in corresponding " mouth " serum sample-adding unit 0,1,2,3 serum to be loaded pipe by the individual unit of three (many) adds 0.5ml serum;" 0;; 0,1,2,3 blood cell in blood cell sample-adding unit is loaded pipe and adds each 0.3ml of blood cell suspension; i.e. whole process only needs 8 sub-samplings, sample-adding process; operator can be loaded by arrangement; brains is not clearly random (this device can arrange standby in advance usually, meet when emergency treatment need to be used immediately and reach easily and efficiently effect).
The subsidiary book membrane vesicle of reaction tube in the present invention, is easy to, with each cross reaction of micro- sem observation, whether there is tiny aggegation.Eliminate the original round sample time for turning slide by every teat glass.
Meanwhile, disposable blood transfusion cross-over experiment combination sample injector can retain for future reference after being finished as transfusion bags, be disposed by institute's sense requirement, it is to avoid occur the danger of occupational exposure when staff's cleaning and sterilizing test tube and slide, meet institute's sense requirement.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.It should be understood by those skilled in the art that; the present invention is not limited to the above embodiments; merely illustrating the principles of the invention described in above-described embodiment and specification; without departing from the spirit and scope of the present invention; various changes and modifications of the present invention are possible, and these changes and improvements all fall within the protetion scope of the claimed invention.The scope of the present invention is defined by the appended claims and its equivalents.
Claims (1)
- Claim1st, the blood mathcing test unit for cross match blood test of disposably transfusing blood, including transparent developmental tube, it is characterized in that, the blood mathcing test unit also includes getting rid of two sample-adding pipes in increase serum and blood cell respectively, the transparent developmental tube is a seal cavity, and two sample-addings pipe is connected conducting with developmental tube.2nd, it is according to claim 1 to get rid of in the blood mathcing test unit of disposably blood transfusion cross match blood test, including transparent developmental tube, it is characterised in that the developmental tube is made up of polyethylene transparent plastic, its a diameter of 1.2cm, long 4cm.3rd, it is according to claim 1 to be used for the blood mathcing test unit of disposably blood transfusion cross match blood test, including transparent developmental tube, it is characterised in that the blood mathcing test unit also includes being used to show！[the observation portion of sem observation.4th, it is according to claim 3 to be used for the blood mathcing test unit of disposably blood transfusion cross match blood test, including transparent developmental tube, it is characterised in that the observation portion is the transparent developmental tube of flat.5th, according to claim 3 to be used for the blood mathcing test unit of disposably blood transfusion cross match blood test, including transparent developmental tube, it is characterised in that the observation portion is transparent experiment bottom of the tube, the transparent experiment bottom of the tube is flat.6th, it is according to claim 3 to be used for the blood mathcing test member of disposably blood transfusion cross match blood test, wrap the transparent developmental tubes of ^ ^, it is characterised in that the observation portion is sealed thin transparent membrane vesicle, the thin transparent membrane vesicle is connected conducting with developmental tube.7th, it is according to claim 6 to be used for the blood mathcing test unit of disposably blood transfusion cross match blood test, including transparent developmental tube, it is characterised in that to state thin transparent membrane vesicle for oblate shape, be made of one piece with developmental tube.8th, it is according to claim 6 to be used for the blood mathcing test unit of disposably blood transfusion cross match blood test, including transparent developmental tube, it is characterised in that the thin transparent membrane vesicle and experiment！^ junction is away from lc nio at the top of developmental tube9th, it is according to claim 1 be used to disposably transfuse blood intersect the blood mathcing test unit with experiment, including transparent developmental tube, it is characterised in that two sample-addings pipe connect to be formed ' " type, the public end in its bottom connects conducting with developmental tube.10th, the Xiang according to claim 1 or 9 is in the blood mathcing test of disposably blood transfusion cross match blood test Unit, including transparent developmental tube, it is characterised in that the sample-adding pipe is integrally formed with developmental tube.11st, it is used for the blood mathcing test unit of disposably blood transfusion cross match blood test according to claim 1 or 9, the sample-adding pipe is docked by spiro connection structure with developmental tube or docked by seccotine.12nd, it is used for the blood mathcing test unit of disposable defeated cross match blood test according to claim 1 or 9, the shape and/or color of two sample-addings pipe are different, correspondence serum sample-adding and blood cell sample-adding.13rd, it is used for the blood mathcing test unit of disposably blood transfusion cross match blood test according to claim 1 or 9, the sections of two sample-addings Guan Zhongyi sample-adding pipes are circle, and another section for being loaded pipe is square.14th, it is used for the blood mathcing test unit of disposably blood transfusion cross match blood test according to claim 1 or 9, the entrance points of two sample-addings pipe set structure of having any different. ,15th, it is used for the blood mathcing test unit of disposably blood transfusion cross match blood test according to what claim 14 was stated, the difference structure is that the entrance point of a sample-adding pipe is spherical or cylindric, and the entrance point of another sample-adding pipe is column or cube shape.16th, the blood mathcing test combiner for cross match blood test of disposably transfusing blood, it is characterised in that the combiner includes N2Blood mathcing test unit any one of individual claim 1 to 9, N is match person's number, N >=2, circulation pairing is carried out to the serum sample-adding pipe on all blood mathcing test units and blood cell sample-adding pipe according to cross match blood test principle, so that all serum sample-adding pipes correspond to the serum of corresponding match person respectively, all blood cell sample-adding pipes correspond to the blood cell of corresponding match person respectively, and combine the serum sample-adding pipe of the identical serum of correspondence, form N number of serum sample-adding unit, the blood cell sample-adding pipe of the identical blood cell of correspondence is combined, form N number of blood cell sample-adding unit.17th, it is according to claim 16 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterised in that during the circulation pairing, by N2Individual blood mathcing test cell-average is divided into N number of blood mathcing test unit group, each blood mathcing test unit group includes N number of blood mathcing test unit, the blood cell sample-adding pipe that serum sample-adding pipe in wherein each blood mathcing test unit group on N number of blood mathcing test unit is grouped together on N number of serum sample-adding unit, each blood mathcing test unit during word in each blood mathcing test unit group is grouped together into N number of blood cell sample-adding unit with the blood cell sample-adding pipe on a certain blood mathcing test unit of other all blood mathcing test unit groups respectively.18th, it is according to claim 16 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterised in that during the circulation pairing, by N2Individual blood mathcing test cell-average is divided into N number of blood mathcing test unit group, and each blood mathcing test unit group includes N number of blood mathcing test member, wherein each match is tried Blood cell sample-adding pipe in verification certificate tuple on N number of blood mathcing test unit is grouped together into N number of blood cell sample-adding units, while the serum sample-adding pipe on each blood mathcing test unit in each blood mathcing test unit group is grouped together into N number of serum sample-adding unit with the serum sample-adding pipe on a certain match examination unit of other all blood mathcing test unit groups respectively.19th, it is according to claim 16 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterized in that, as N=2, the serum sample-adding pipe and blood cell sample-adding pipe that each blood mathcing test unit group is included on 1 blood mathcing test unit, two blood mathcing test units are respectively combined to form serum sample-adding unit and blood cell sample-adding unit.20th, it is according to claim 16 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterized in that, the blood mathcing test combiner also includes a test stand for being used to fix blood mathcing test unit, the test stand includes a plurality of fixed plates and a fixed mount, offered in the fixed plate with the fixing hole that developmental tube is engaged in blood mathcing test unit, be provided with the fixed mount and be engaged slot with fixed plate.21st, it is according to claim 20 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterised in that the slot arrays distribution.22nd, it is according to claim 20 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterized in that, a plurality of breach are radially offered on the fixing hole of the fixed plate, fixing hole is split along side to form a plurality of fixing tooths.23rd, it is according to claim 22 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterised in that the bottom of the breach is circular hole.24th, it is used for the blood mathcing test combiner of disposably blood transfusion cross match blood test according to claim 22 or 23, it is characterised in that the breach is symmetrical.25th, it is according to claim 20 to be used for the blood mathcing test combiner of disposably blood transfusion cross match blood test, it is characterised in that the fixed plate and fixed mount are made up of plastics, lucite or metal material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011800434378A CN103299194A (en) | 2011-01-19 | 2011-12-12 | Blood matching unit and combiner for disposable blood transfusion and cross matching experiment |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110021793.1A CN102608336B (en) | 2011-01-19 | 2011-01-19 | Disposable transfusion and cross matching experiment combiner |
| CN201110021793.1 | 2011-01-19 | ||
| CN2011800434378A CN103299194A (en) | 2011-01-19 | 2011-12-12 | Blood matching unit and combiner for disposable blood transfusion and cross matching experiment |
| PCT/CN2011/002082 WO2012097494A1 (en) | 2011-01-19 | 2011-12-12 | Blood matching unit and combiner for disposable blood transfusion and cross matching experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103299194A true CN103299194A (en) | 2013-09-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110021793.1A Active CN102608336B (en) | 2011-01-19 | 2011-01-19 | Disposable transfusion and cross matching experiment combiner |
| CN2011800434378A Pending CN103299194A (en) | 2011-01-19 | 2011-12-12 | Blood matching unit and combiner for disposable blood transfusion and cross matching experiment |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110021793.1A Active CN102608336B (en) | 2011-01-19 | 2011-01-19 | Disposable transfusion and cross matching experiment combiner |
Country Status (2)
| Country | Link |
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| CN (2) | CN102608336B (en) |
| WO (1) | WO2012097494A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614538A (en) * | 2015-02-13 | 2015-05-13 | 刘大基 | Multifunctional disposable transfusion and cross blood-matching test combiner |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108593942B (en) * | 2018-04-17 | 2020-09-08 | 杭州电子科技大学 | Cross blood matching method |
| CN109283037A (en) * | 2018-09-03 | 2019-01-29 | 万峰春 | A kind of crossmatch verifying attachment |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN102608336B (en) | 2014-12-24 |
| WO2012097494A1 (en) | 2012-07-26 |
| CN102608336A (en) | 2012-07-25 |
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Application publication date: 20130911 |