CN103439518B - A kind of blood ABD shapes rapid integrated test card - Google Patents
A kind of blood ABD shapes rapid integrated test card Download PDFInfo
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- CN103439518B CN103439518B CN201310227495.7A CN201310227495A CN103439518B CN 103439518 B CN103439518 B CN 103439518B CN 201310227495 A CN201310227495 A CN 201310227495A CN 103439518 B CN103439518 B CN 103439518B
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Abstract
The invention discloses a kind of blood ABD to shape rapid integrated test card, the deck (21) of this test card is provided with multiple draw-in groove, the plurality of draw-in groove is divided into many groups of sequential, many groups draw-in groove sequentially corresponds to blood group positive definite form detection zone (1), blood group reverse type detection zone (2) and cross matching detection zone (3), is provided with the test strips being matched with its detection zone in each draw-in groove; This Ka Gai has well (6) and flushing port (7) successively on the position corresponding to the upper blood group positive definite form detection zone (1) of deck (21), blood group reverse type detection zone (2) and cross matching detection zone (3) each test strips.Blood group positive definite form detects by the present invention, reverse type detects, cross matching detects and comprehensively detects to a card simultaneously, detection time is short, easy to carry, simple to operate, result accurately and reliably, be applicable to the comprehensive detection of preliminary blood screening and blood typing, before also can be used for blood transfusion in very military period, blood typing is tested.
Description
Technical field
The invention belongs to blood testing technical field, be specifically related to a kind of blood ABD and shape rapid integrated test card.
Background technology
Current Blood grouping reagent strip on the market often unitem detects, and it is long to there is detection time, and testing cost is high, the shortcoming of inconvenient operation.Positive definite form detects and adopts agglutination, complicated operation more at present, easily pollutes.And reverse type detects employing test tube method or flat band method usually, the reagent red blood cell used is prepared with blood unit voluntarily primarily of each, there is no unified standard, the preparation method of constituent parts is also different, alserver's solution composition and physicochemical property are all variant, cause the quality of reverse type red blood cell reagent extremely unstable, and the storage in vitro phase are short, be generally 1-3 month, this greatly limits its application in actual clinical.Cross matching common method has salt water law, pawpaw enzyme process, cohesion amine method, antiglobulin method, micro-column gel agglutination assay.Salt water law is not easy to detect incomplete antibody, pawpaw enzyme process, cohesion amine method, antiglobulin method, and the micro-column gel agglutination assay reaction time is long, and complex operation is expensive, relates to instrument and uses.
Summary of the invention
The object of this invention is to provide one and cover blood group positive definite form detection, reverse type detects the shape blood ABD of testing result of interpretation blood examination that is easy to detected with cross matching and to shape rapid integrated test card, this test card is without the need to instrument, simple to operate, can in 10min naked eyes sentence read result, detection time is short, and result accurately and reliably.
The object of the invention is to realize in the following manner:
A kind of blood ABD shapes rapid integrated test card, this test card comprises deck and is fastened on the Ka Gai on deck, this deck is provided with multiple draw-in groove, the plurality of draw-in groove is divided into many groups of sequential, many groups draw-in groove sequentially corresponds to blood group positive definite form detection zone, blood group reverse type detection zone and cross matching detection zone, is provided with the test strips being matched with its detection zone in each draw-in groove; This Ka Gai all has well and flushing port on the position corresponding to blood group positive definite form detection zone, each test strips in blood group reverse type detection zone and cross matching detection zone on deck.
The plurality of draw-in groove is arranged in parallel 6, it comprises the first draw-in groove, the second draw-in groove, the 3rd draw-in groove, the 4th draw-in groove, the 5th draw-in groove, the 6th draw-in groove, this first draw-in groove, the second draw-in groove and the 3rd draw-in groove correspond to blood group positive definite form detection zone, 4th draw-in groove, the 5th draw-in groove correspond to blood group reverse type detection zone, and the 6th draw-in groove corresponds to cross matching detection zone.
On the position corresponding to each test strips in blood group positive definite form detection zone on deck, result show hole is also had at Ka Gai.On the position corresponding to each test strips in blood group reverse type detection zone on deck, result show hole and product identification label hole is also had at Ka Gai.
The test strips of cross matching detection zone comprises thieving paper, first cushion pad, second cushion pad, antibody pad, washing fluid dropping pad and the polyester sheet as backing, washing fluid drips pad and is placed on polyester sheet edge, one end of first cushion pad rides over washing fluid and drips on pad, antibody pad rides on the other end of first cushion pad, one end of second cushion pad rides on antibody pad, and thieving paper rides on the other end of second cushion pad.
In test strips, the position at antibody pad place corresponds to the well of Ka Gai, and the position at washing fluid application of sample pad place corresponds to the flushing port of Ka Gai.
Thieving paper, washing fluid application of sample pad, first cushion pad, second cushion pad, antibody cushion material are made up of the porous polymer materials with certain pore size, and pore size at least allows single red blood cell to pass through.Described hole macromolecular material is preferably glass fibre.
In the well that each project is corresponding, add the whole blood sample of 10ul when detection, then drip the auxiliary liquid of 2 to 3 (about 100ul-150ul) in the flushing port of correspondence, after 10 minutes, get final product sentence read result.
It is solid phase assay that blood group positive definite form detects what adopt, fiberglass packing bag by blood cell protein, by the change of reacted color, Preliminary detection judgement is carried out to the blood group of human whole blood.The interpretation mode of positive definite form detection zone is: the redness of well is not taken off, and represents that examined samples are with corresponding blood group antigens, such as: in Fig. 1, A, B, D item is all aobvious red, then representing examined samples positive definite form testing result is blood group AB type, and RhD is positive.The interpretation mode of reverse type detection zone is: C line color band, represent that this detects effectively, there is obvious colour band in T line, then represent the antibody of examined samples with correspondence, such as A item display colour band, B item does not show colour band, then represent examined samples with A antibody, and blood group reverse type testing result is blood group Type B.
What blood group reverse type detected employing is Colloidal Gold, the blood group antigens of colloid gold label are fixed on glass fibre membrane, during reaction, antibody in blood and its reaction bonded form compound, reacted again by chromatography and the blood group antigens be fixed on nitrocellulose membrane, form immune complex, therefore, observe the formation colour band whether detection line having antigen antibody complex, can blood typing interpretation be carried out.
Quick cross matching approach application to methodology be solid phase assay, solid phase assay is a kind of biological respinse method of carrying out on solid phase carrier, color change after can being reacted on carrier by reactant or composition transfer, carry out interpretation to testing result.
The present invention can use instrument to carry out result interpretation, and some combinations wherein can be selected to test, and also blood group reverse type can be detected the collaurum inspection test-strips used and unite two into one, colloid gold test strip carries out anti-A antibody simultaneously and anti-B antibody detects.
Beneficial effect of the present invention compared with the prior art: blood group positive definite form detects by the present invention, blood group reverse type detects, blood cross matching detects and comprehensively detects to a card simultaneously, blood screening testing result is drawn in ten minutes, detection time is short, easy to carry, simple to operate, blood examination sizing testing result accurately and reliably, be applicable to preliminary blood screening when taking a blood sample and the comprehensive detection of blood typing, also can be used for blood typing test before very military period or major disaster blood transfusion in period.
Accompanying drawing explanation
Fig. 1 is blood sizing comprehensive detection card card face structural representation.
Fig. 2 is blood sizing comprehensive detection card cartridge structure schematic diagram.
Fig. 3 is cross matching test strip structural representation.
In figure, 1 is blood group positive definite form detection zone; 2 is blood group reverse type detection zone; 3 for cross matching detection zone, 4 be result show hole, 5 for product identification label hole, 6 for well, 7 be flushing port, 8 for polyester sheet, 9 for thieving paper, 10 for washing fluid drip pad, 11 for first cushion pad, 12 be second cushion pad, 13 be antibody pad, 14 is the first draw-in groove, 15 is the second draw-in groove, and 16 is the 3rd draw-in groove, and 17 is the 4th draw-in groove, 18 is the 5th draw-in groove, 19 is the 6th draw-in groove, and 20 is Ka Gai, and 21 is deck.
Embodiment
Below by specific embodiment, the present invention is further elaborated:
As Figure 1-3, a kind of blood ABD shapes rapid integrated test card, this test card comprises deck 21 and is fastened on the card lid 20 on deck 21, this deck 21 is provided with multiple draw-in groove, the plurality of draw-in groove is divided into many groups of sequential, many groups draw-in groove sequentially corresponds to blood group positive definite form detection zone 1, blood group reverse type detection zone 2 and cross matching detection zone 3, is provided with the test strips being matched with its detection zone in each draw-in groove; This card lid 20 has well 6 and flushing port 7 successively on the position corresponding to blood group positive definite form detection zone 1, each test strips in blood group reverse type detection zone 2 and cross matching detection zone 3 on deck 21.
The plurality of draw-in groove is arranged in parallel 6, it comprises the first draw-in groove 14, second draw-in groove 15, the 3rd draw-in groove 16, the 4th draw-in groove 17, the 5th draw-in groove 18 and the 6th draw-in groove 19, this the first draw-in groove 14, second draw-in groove 15 and the 3rd draw-in groove 16 correspond to blood group positive definite form detection zone 1,4th draw-in groove 17, the 5th draw-in groove should correspond to cross matching detection zone 3 in blood group reverse type detection zone the 2, six draw-in groove 19 to 18; On the position corresponding to each test strips in blood group positive definite form detection zone 1 on deck 21, result show hole 4 is also had at card lid 20.On the position corresponding to each test strips in blood group reverse type detection zone 2 on deck 21, result show hole 4 and product identification label hole 5 is also had at card lid 20.
The test strips of cross matching detection zone 3 comprises thieving paper 9, first cushion pad 11, second cushion pad 12, antibody pad 13, washing fluid dropping pad 10 and the polyester sheet 8 as backing, washing fluid drips pad 10 and is placed on polyester sheet 8 edge, one end of first cushion pad 11 rides over washing fluid and drips on pad 10, antibody pad 13 rides on the other end of first cushion pad 11, one end of second cushion pad 12 rides on antibody pad 13, and thieving paper 9 rides on the other end of second cushion pad 12.
In test strips, the position at antibody pad 13 place corresponds to the well 6 on card lid 20, and the position of washing fluid application of sample pad 10 corresponds to the flushing port 7 blocking and cover.Thieving paper 9, washing fluid application of sample pad 10, first cushion pad 11, second cushion pad 12, antibody pad 13 are made up of the porous polymer materials with certain pore size, and pore size at least allows single red blood cell to pass through.Described hole macromolecular material is preferably glass fibre.
In the well that each project is corresponding, add the whole blood sample of 10ul when detection, in the flushing port of correspondence, drip the auxiliary liquid of 2 to 3 (about 100ul-150ul) afterwards, after 10 minutes, get final product sentence read result.
Adopt said structure, blood group positive definite form detects by the present invention, and reverse type detects, cross matching detects and comprehensively detects to a card simultaneously, and in ten minutes, draw blood screening testing result, detection time is short, easy to carry, simple to operate, blood examination sizing testing result accurately and reliably.
It is solid phase assay that blood group positive definite form detects what adopt, fiberglass packing bag by blood cell protein, by the change of reacted color, Preliminary detection judgement is carried out to the blood group of human whole blood.The interpretation mode of positive definite form detection zone is: the redness of well is not taken off, and represents that examined samples are with corresponding blood group antigens, such as: in Fig. 1, A, B, D item is all aobvious red, then representing examined samples positive definite form testing result is blood group AB type, and RhD is positive.The interpretation mode of reverse type detection zone is: C line color band, represent that this detects effectively, there is obvious colour band in T line, then represent the antibody of examined samples with correspondence, such as A item display colour band, B item does not show colour band, then represent examined samples with A antibody, and blood group reverse type testing result is blood group Type B.
What blood group reverse type detected employing is Colloidal Gold, the blood group antigens of colloid gold label are fixed on glass fibre membrane, during reaction, antibody in blood and its reaction bonded form compound, reacted again by chromatography and the blood group antigens be fixed on nitrocellulose membrane, form immune complex, therefore, observe the formation colour band whether detection line having antigen antibody complex, can blood typing interpretation be carried out.
It is solid phase assay that cross matching detects the methodology applied to, test-strips is coated with anti-ig M, IgG, the first sample loading alternative, during master reaction: first add blood donor's serum (or blood plasma or whole blood) at well, reaction a period of time, add blood donor's red blood cell or (whole blood), reaction a period of time, add washing fluid in flushing port (i.e. test-strips one end); During secondary side reaction: first add blood donor's serum (or blood plasma or whole blood) at well, reaction a period of time, add blood donor's red blood cell or (whole blood), reaction a period of time, add washing fluid in flushing (hole and test-strips one end); Make not with the red blood cell of antibody response under the springing up of washing fluid away from adding sampling point place, and be left in the red blood cell of antibody generation immune agglutination and add sampling point place, then can according to whether reaction site has, the red blood cell of aggegation is residual judge that antibody and red blood cell react.If master reacts and just can not transfuse blood, secondary side reacts and depending on circumstances determines blood transfusion again.The second sample loading alternative, blood donor's whole blood and blood donor's whole blood or blood donor's serum and blood donor's red blood cell or blood donor sera and blood donor's red blood cell are first placed in test tube and react a period of time, get a certain amount of red blood cell mixed liquor in test tube again to well (i.e. test-strips add sampling point place), reaction a period of time, add washing fluid in flushing port (i.e. test-strips one end); Make not with the red blood cell of antibody response under the springing up of washing fluid away from adding sampling point place, and be left in the red blood cell of antibody generation immune agglutination and add sampling point place, then can judge that antibody and red blood cell react according to whether reaction site there being the red blood cell of aggegation remain, can transfuse blood if reactionless, master reaction can not be transfused blood, and secondary side reaction depending on circumstances determines blood transfusion again.
Claims (5)
1. a blood ABD shapes rapid integrated test card, this test card comprises deck (21) and is fastened on the Ka Gai (20) on deck (21), (21) are provided with multiple draw-in groove to this deck, the plurality of draw-in groove is divided into many groups of sequential, it is characterized in that, many groups draw-in groove sequentially corresponds to blood group positive definite form detection zone (1), blood group reverse type detection zone (2) and cross matching detection zone (3), is provided with the test strips being matched with its detection zone in each draw-in groove, this Ka Gai (20) all has well (6) and flushing port (7) on the position corresponding to the upper blood group positive definite form detection zone (1) of deck (21), blood group reverse type detection zone (2) and cross matching detection zone (3) each test strips, wherein, the test strips of cross matching detection zone (3) comprises thieving paper (9), first cushion pad (11), second cushion pad (12), antibody pad (13), washing fluid drips pad (10) and the polyester sheet (8) as backing, washing fluid drips pad (10) and is placed on polyester sheet (8) edge, one end of first cushion pad (11) rides over washing fluid and drips on pad (10), antibody pad (13) rides on the other end of first cushion pad (11), one end of second cushion pad (12) rides on antibody pad (13), thieving paper (9) rides on the other end of second cushion pad (12), in test strips, the position at antibody pad (13) place corresponds to the well (6) on Ka Gai (20), and the position at washing fluid application of sample pad (10) place corresponds to the flushing port (7) on Ka Gai (20).
2. blood ABD according to claim 1 shapes rapid integrated test card, it is characterized in that, the plurality of draw-in groove is arranged in parallel 6, it comprises the first draw-in groove (14), second draw-in groove (15), 3rd draw-in groove (16), 4th draw-in groove (17), 5th draw-in groove (18) and the 6th draw-in groove (19), this first draw-in groove (14), second draw-in groove (15) and the 3rd draw-in groove (16) are corresponding to blood group positive definite form detection zone (1), 4th draw-in groove (17), 5th draw-in groove (18) is corresponding to blood group reverse type detection zone (2), 6th draw-in groove (19) is corresponding to cross matching detection zone (3).
3. blood ABD according to claim 1 shapes rapid integrated test card, it is characterized in that, has result show hole (4) at Ka Gai (20) on the position corresponding to upper blood group positive definite form detection zone (1) each test strips of deck (21).
4. blood ABD according to claim 1 shapes rapid integrated test card, it is characterized in that, on the position corresponding to upper blood group reverse type detection zone (2) each test strips of deck (21), have result show hole (4) and product identification label hole (5) at Ka Gai (20).
5. blood ABD according to claim 1 shapes rapid integrated test card, it is characterized in that thieving paper (9), washing fluid application of sample pad (10), first cushion pad (11), second cushion pad (12), antibody pad (13) be made up of the porous polymer materials with certain pore size, pore size at least allows single red blood cell to pass through.
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| CN103439518B true CN103439518B (en) | 2015-09-02 |
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Families Citing this family (6)
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|---|---|---|---|---|
| CN103364571B (en) * | 2013-06-24 | 2016-01-20 | 英科新创(厦门)科技有限公司 | A kind of cross matching detection method and examination bar |
| CN103926400A (en) * | 2014-04-06 | 2014-07-16 | 邵超鹏 | Test strip for specifically measuring IgM, IgG and IgA blood group antibodies |
| CN109459575A (en) * | 2018-09-19 | 2019-03-12 | 厦门为正生物科技股份有限公司 | A kind of ABO&RhD blood group detection device |
| CN112578129A (en) * | 2020-12-05 | 2021-03-30 | 西安良升生物科技有限公司 | ABO positive and negative typing/RhD blood type detection micro-disc |
| CN113634295B (en) * | 2021-09-14 | 2022-11-04 | 南京岚煜生物科技有限公司 | Microfluidic blood type detection chip |
| CN114324957B (en) * | 2022-03-16 | 2022-05-20 | 天津德祥生物技术有限公司 | Blood type positive and negative shaping sample adding card and sample adding assembly |
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Address after: 210002 Zhongshan East Road, Jiangsu, China, No. 305, No. Co-patentee after: Yingke Xinchuang (Xiamen) Science and Technology Co., Ltd. Patentee after: Nanjing General Hospital of Nanjing Military Region of the People's Liberation Army of China Address before: 210002 Zhongshan East Road, Jiangsu, China, No. 305, No. Co-patentee before: Yingke Xinchuang (Xiamen) Technology Co., Ltd. Patentee before: Nanjing General Hospital of Nanjing Military Region of the People's Liberation Army of China |