CN103479351A - Electrophysiological recording device - Google Patents
Electrophysiological recording device Download PDFInfo
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- CN103479351A CN103479351A CN201310450212.5A CN201310450212A CN103479351A CN 103479351 A CN103479351 A CN 103479351A CN 201310450212 A CN201310450212 A CN 201310450212A CN 103479351 A CN103479351 A CN 103479351A
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Abstract
An electrophysiological recording device comprises a guide tube component, an electrode component and a sample feeding component. The guide tube component comprises a guide tube holding marking dye. The electrode component is used for acquiring the position of the marking dye and coupled to one end of the guide tube. The sample feeding component is connected with the other end of the guide tube and pushes the marking dye which is then guided out. The electrophysiological recording device is available for marking the position an electrode accurately.
Description
Technical field
The invention belongs to the Electrophysiology technical field, relate in particular to a kind of electrophysiological recording device.
Background technology
The Electrophysiology technology is the research neuroscience, resolves the important tool of cerebral nerve network.Take classical patch clamp technique as example, macroscopic view, it can study the neuron contact in two brain intervals; Microcosmic, it can record the activity of single ion channel, makes people reach the unthinkable stage of forefathers to neuronic understanding.Yet nervous system has cooperated jointly in hundreds of millions neuronic specific connections and coded system.Therefore the understanding of whole nervous system and brain function is also known little about it.Along with going deep into of the research of the working mechanism to neutral net, people make up the deficiency of existing electrophysiological recording technology in the urgent need to new method.
With respect to classical patch-clamp electrophysiological technique, multichannel electrophysiological recording technology is to adopt the method for extracellular recording to monitor the central a plurality of neuronic synchronous electric activities of neuron pool.Apply a large amount of neuronic electrical activity that this method can a plurality of brains of synchronous recording district, the electrophysiological function contact of being convenient to study between Different brain region is even individual when accepting stimulation or carrying out a certain specific behavior task, the contact of the neuron of Different brain region electric discharge on time and space, and then study the working mechanism of neutral net in brain by analyzing neuronic discharge mode.With patch clamp technique, compare, its advantage is to record the more huge electrical activity of neurons of quantity on live body.Yet the patch-clamp recording system can be used jointly with micro imaging system usually, utilizes the fluorescent labeling technology, can optionally to the neuron of ad-hoc location and type, be studied.And the method that the multiple recording technology is located by brain map usually is implanted to the target location line item of going forward side by side by electrode.Yet owing to there being the factors such as individual variation, in experiment, may there be deviation in practical study Nao district with target area, and the electrical activity of neurons that physical record arrives may be not from target core group.
The uncertain of this electrophysiological recording position just may be brought the even wrong information of some errors in the middle of research, allows an experimental result need more work repeatedly to confirm, greatly increases workload.Even may be because the deviation of position gets the wrong sow by the ear.In order to address this problem, people can, after electrophysiological recording finishes, adopt the method for brain sheet tissue staining to be verified at present.
Use multichannel in the middle of the research of body electrophysiological recording at many needs, people just give target location out, and the mode of then repeatedly verifying by many experiments gets rid of as far as possible because the inaccurate adverse effect caused of record position.This method not only increases the workload of experimentation, and can not shield the result error that possible position deviation is brought fully.
And, by after the laboratory animal record, carrying out the method that tissue staining is confirmed, in fact have very large breach, especially anaesthetize electrophysiological recording.Be taken out to dyeing from electrode and also have much to operation and a very long time from cerebral tissue, have a lot of probabilities in this process, for example electrode takes out may have other damage to cerebral tissue; Cerebral tissue is flexible, can do by myself and draws in after electrode takes out; The damage that in short time, the electrode pair cerebral tissue causes is large not, even if dyeed, can not well it be identified; The direction of section and the track of bottom electrode not in one plane, as long as a little deviation just can not be seen complete electrode vestige, can only judge that at most whether the direction of bottom electrode is correct, and are difficult to the position of judgement eletrode tip.
Summary of the invention
Based on this, be necessary to provide a kind of electrophysiological recording device of the electrode position of labelling comparatively accurately.
A kind of electrophysiological recording device comprises:
Conduit tube component, comprise conduit, in described conduit, has labeling dye;
Electrode assemblie, for gathering the position of labeling dye, an end coupling of described electrode assemblie and described conduit; And
The sample introduction assembly, be connected with the other end of described conduit, and provide a motive force to impel described labeling dye to derive to described labeling dye.
Further, described conduit is silicone tube, glass tubing or poly property management; The external diameter of described conduit is 20 microns～500 microns; Described labeling dye is CNT, quantum dot or immunofluorescence dyestuff.
Further, described sample introduction assembly comprises syringe, and described syringe has injection needle, and described conduit is sheathed on described injection needle away from an end of described electrode assemblie.
Further, described sample introduction assembly also comprises and adds the syringe pump be held on described syringe.
Further, described conduit tube component also comprises an end and the described conduit connection tube away from an end socket of described electrode assemblie, and the other end of described connection tube is sheathed on described injection needle.
Further, described connection tube is plastic tube or rubber tube; Be provided with implant in described connection tube, described implant along described connection tube axially slidably; Described implant is inert fluid.
Further, described conduit tube component also comprises sealing shroud, and described sealing shroud is sheathed on the junction of described conduit and described connection tube and seals the junction of described conduit and described connection tube; The material of described sealing shroud is rubber, latex or silica gel.
Further, described electrode assemblie comprises a plurality of electrodes that are arranged in array, and described a plurality of electrodes all are coupled with the end of described conduit away from described sample introduction assembly, and in described a plurality of electrodes, at least one is recording electrode.
Further, also has fibre electrode in described a plurality of electrode.
Further, also has stimulating electrode in described a plurality of electrode.
Above-mentioned electrophysiological recording device comprises conduit tube component, electrode assemblie and sample introduction assembly, when using this electrophysiological recording device to carry out labelling to cerebral tissue, one end of the conduit of electrode assemblie and conduit tube component is all inserted to cerebral tissue, by the sample introduction assembly, the labeling dye in conduit is derived, can gather the position of labeling dye with the electrode assemblie of conduit coupling, thereby the location records that electrophysiological recording is finished to that time gets off, thereby to determine the actual position of eletrode tip in the electrophysiological recording experiment, therefore, above-mentioned electrophysiological recording device is the position of labelling electrode comparatively accurately.
The accompanying drawing explanation
The electrophysiological recording electrode that Fig. 1 is an embodiment has omitted the structural representation of electrode assemblie;
The electrode assemblie that Fig. 2 is the electrophysiological recording electrode shown in Fig. 1 and the structural representation of conduit;
The structural representation of the conduit tube component that Fig. 3 is the electrophysiological recording electrode shown in Fig. 1;
The structural representation of the conduit that Fig. 4 is the electrophysiological recording electrode shown in Fig. 3;
The structural representation of the electrode assemblie that Fig. 5 is the electrophysiological recording electrode shown in Fig. 2 and another angle of conduit.
The specific embodiment
In order to make purpose of the present invention, technical scheme and advantage more clear, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Refer to Fig. 1 and Fig. 2, the electrophysiological recording device 10 of an embodiment, comprise conduit tube component 100, electrode assemblie 200 and sample introduction assembly 300.
See also Fig. 3, conduit tube component 100 comprises conduit 110, has labeling dye 112 in conduit 110.As shown in Figure 4, conduit 110 is derived labeling dye 112 is arranged.Conduit 110 can be the comparatively solid and hard tube of material, is preferably silicone tube, glass tubing or poly property management, and wherein, the poly property management can be polyurethane tube, polyfluortetraethylene pipe.The conduit of these materials is durable and hard, after can avoiding fractureing or entering cerebral tissue, because of crooked can't with the electrode target approach zone, ground that is consistent, and the good leak tightness of the sidewall of the conduit of these materials, can not make labeling dye 112 leak.Further, in conduit 110, can also store other dyestuff, for example, the medicine that not affected by labeling dye 112.The external diameter of conduit 110 is 20 microns～500 microns.Wherein, labeling dye 112 can be the commonly used labeling dye 112 that is not easy diffusion and washing in this area, be preferably CNT quantum dot or immunofluorescence dyestuff, wherein, immunofluorescence label dyestuff 112 can be for self with fluorescence other labeling dye 112 with " antigen " of fluorescence that maybe can be coupled; And the color of labeling dye 112 can be black, or other color.Further, conduit tube component 100 also comprises an end and conduit 110 connection tube 120 away from an end socket of electrode assemblie 200.Connection tube 120 is pliability long tube preferably.Connection tube 120 is plastic tube or rubber tube.Further, in connection tube 120, be provided with implant, this implant along connection tube 120 axially slidably.Wherein, implant be can sealed joint pipe material, be preferably inert fluid, for example, paraffin oil, silicone oil.Further, conduit tube component 100 also comprises sealing shroud 130, and sealing shroud 130 is sheathed on conduit 110 and the junction of connection tube 120 junction of sealing duct 110 and connection tube 120.By being set, sealing shroud 130 can prevent that liquid in conduit tube component 100 from revealing from the gap of conduit 110 and the junction of connection tube 120.Wherein, sealing shroud 130 is good and have elastic material for air-tightness, is preferably rubber, latex or silica gel.Be appreciated that, sealing shroud 130 can omit, and conduit 110 can also adopt the alternate manner sealing with the junction of connection tube 120, for example, apply in the gap of conduit 110 and the junction of connection tube 120 junction with sealing duct 110 with connection tube 120 with glue that can be curing; Or even connection tube 120 also can omit, now, conduit 110 directly is connected with sample introduction assembly 300 away from an end of electrode assemblie 200.
See also Fig. 2 and Fig. 5, electrode assemblie 200 is for gathering electricity physiological signal.Electrode 200 comprises a plurality of electrodes that are arranged in array 210, and a plurality of electrodes 210 all are coupled with the end of conduit 110 away from sample introduction assembly 300.Wherein, in a plurality of electrodes 210, at least one is recording electrode.Be appreciated that electrode assemblie 200 also can only include an electrode 210, and this electrode 200 is recording electrode.Preferably, also have fibre electrode in a plurality of electrodes 210, by fibre electrode is set, posting field is carried out to photostimulation, can carry out to posting field the function of photostimulation, thereby recording light stimulates and the electrophysiological change of front and back.Further, in a plurality of electrodes 210, also stimulate electrode, by stimulating electrode is set, posting field is carried out to electricity irritation, thereby can observe the neuron variation that electricity irritation is induced, as excited as quilt or inhibition, or change neuron coded system etc.Be appreciated that a plurality of electrodes 210 can only have recording electrode and fibre electrode; Also can only have recording electrode and stimulating electrode; Can be also that recording electrode, fibre electrode and stimulating electrode are arranged simultaneously; Or even, in a plurality of electrodes 210, the electrode of recording electrode, fibre electrode, stimulating electrode and other function can also be arranged simultaneously, thereby make this electrode assemblie 200 there is more function.
Please again consult Fig. 1, sample introduction assembly 300 is connected with the other end of conduit 110, and provides a motive force to impel labeling dye 112 to derive to labeling dye 112.Sample introduction assembly 300 comprises syringe 310.Syringe 310 has injection needle 312, and conduit 110 is sheathed on injection needle 312 away from an end of electrode assemblie 200.In specific embodiment, the end away from conduit 110 of connection tube 120 is sheathed on injection needle 312.Further, sample introduction assembly 300 also comprises and adds the syringe pump 310 be held on syringe 310.Syringe pump 310 can be controlled derivation speed and the total amount of syringe 310 with control mark dyestuff 112, thus the derivation of control mark dyestuff 112 more accurately.Wherein, syringe 310 is microsyringe, and syringe pump 320 is micro-injection pump, thereby more accurately labeling dye 112 is derived from conduit 110 to inject cerebral tissue.Be appreciated that syringe pump 320 also can omit; In addition, sample introduction assembly 300 also can not taked the mode of syringe 310, also can adopt alternate manner that labeling dye 112 is derived from conduit 110.
Further, conduit tube component 100 also comprises the sealing member (not shown) of the junction of sealed joint pipe 120 and injection needle 312, thereby can well seal connection tube 120 and the junction of injection needle 312.Be appreciated that sealing member can omit, can adopt the junction that can curing glue be coated in connection tube 120 and injection needle 312.
Above-mentioned electrophysiological recording device 10 comprises conduit tube component 100, electrode assemblie 200 and sample introduction assembly 300, when using 10 pairs of cerebral tissue of this electrophysiological recording device to carry out labelling, electrode assemblie 200 and an end of the conduit 110 of conduit tube component 100 are all inserted to cerebral tissue, by sample introduction assembly 300, the labeling dye 112 in conduit 110 is derived, can gather the position of labeling dye 112 with the electrode assemblie 200 of conduit 110 coupling, thereby the location records that electrophysiological recording is finished to that time gets off, thereby to determine the actual position of eletrode tip in the electrophysiological recording experiment.Therefore, above-mentioned electrophysiological recording device 10 position of labelling electrode comparatively accurately.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, although the present invention discloses as above with preferred embodiment, yet not in order to limit the present invention, any those skilled in the art, within not breaking away from the technical solution of the present invention scope, when the technology contents that can utilize above-mentioned announcement is made a little change or is modified to the equivalent embodiment of equivalent variations, in every case be not break away from the technical solution of the present invention content, any simple modification of above embodiment being done according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (10)
1. an electrophysiological recording device, is characterized in that, comprising:
Conduit tube component, comprise conduit, in described conduit, has labeling dye;
Electrode assemblie, for gathering the position of labeling dye, an end coupling of described electrode assemblie and described conduit; And
The sample introduction assembly, be connected with the other end of described conduit, and provide a motive force to impel described labeling dye to derive to described labeling dye.
2. electrophysiological recording device according to claim 1, is characterized in that, described conduit is silicone tube, glass tubing or poly property management; The external diameter of described conduit is 20 microns～500 microns; Described labeling dye is CNT, quantum dot or immunofluorescence dyestuff.
3. electrophysiological recording device according to claim 1, is characterized in that, described sample introduction assembly comprises syringe, and described syringe has injection needle, and described conduit is sheathed on described injection needle away from an end of described electrode assemblie.
4. electrophysiological recording device according to claim 3, is characterized in that, described sample introduction assembly also comprises and adds the syringe pump be held on described syringe.
5. electrophysiological recording device according to claim 3, is characterized in that, described conduit tube component also comprises an end and the described conduit connection tube away from an end socket of described electrode assemblie, and the other end of described connection tube is sheathed on described injection needle.
6. electrophysiological recording device according to claim 5, is characterized in that, described connection tube is plastic tube or rubber tube; Be provided with implant in described connection tube, described implant along described connection tube axially slidably; Described implant is inert fluid.
7. electrophysiological recording device according to claim 5, is characterized in that, described conduit tube component also comprises sealing shroud, and described sealing shroud is sheathed on the junction of described conduit and described connection tube and seals the junction of described conduit and described connection tube; The material of described sealing shroud is rubber, latex or silica gel.
8. electrophysiological recording device according to claim 1, it is characterized in that, described electrode assemblie comprises a plurality of electrodes that are arranged in array, and described a plurality of electrodes all are coupled with the end of described conduit away from described sample introduction assembly, and in described a plurality of electrodes, at least one is recording electrode.
9. electrophysiological recording device according to claim 8, is characterized in that, also has fibre electrode in described a plurality of electrodes.
10. electrophysiological recording device according to claim 8 or claim 9, is characterized in that, also has stimulating electrode in described a plurality of electrodes.
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| CN201310450212.5A CN103479351B (en) | 2013-09-27 | 2013-09-27 | Electrophysiological recording device |
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| CN201310450212.5A CN103479351B (en) | 2013-09-27 | 2013-09-27 | Electrophysiological recording device |
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| CN103479351B CN103479351B (en) | 2015-06-10 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109394211A (en) * | 2018-12-18 | 2019-03-01 | 新乡医学院 | A kind of preparation method in body electrophysiological recording metal electrode |
| CN109394202A (en) * | 2018-12-24 | 2019-03-01 | 浙江中医药大学 | Nerve stimulation record component and preparation method thereof |
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Patent Citations (8)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109394211A (en) * | 2018-12-18 | 2019-03-01 | 新乡医学院 | A kind of preparation method in body electrophysiological recording metal electrode |
| CN109394211B (en) * | 2018-12-18 | 2021-08-06 | 新乡医学院 | Method for preparing in-vivo electrophysiological recording metal electrode |
| CN109394202A (en) * | 2018-12-24 | 2019-03-01 | 浙江中医药大学 | Nerve stimulation record component and preparation method thereof |
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| CN103479351B (en) | 2015-06-10 |
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