CN104266860A - Spring type brain slice sampler and sampling method thereof - Google Patents
Spring type brain slice sampler and sampling method thereof Download PDFInfo
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- CN104266860A CN104266860A CN201410546055.2A CN201410546055A CN104266860A CN 104266860 A CN104266860 A CN 104266860A CN 201410546055 A CN201410546055 A CN 201410546055A CN 104266860 A CN104266860 A CN 104266860A
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- brain sheet
- spring
- suction nozzle
- piston
- bending pipe
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Abstract
The invention discloses a spring type brain slice sampler and a sampling method thereof. The sampler comprises a glass suction head, a bent shape and a controllable piston assembly, wherein a round end of the bent pipe is connected with the controllable piston assembly through a connection head, and the other end of the bent pipe is connected with the glass suction head; and the controllable piston assembly comprises a top, a connecting rod and a piston body part, the upper end of the piston body part is movably connected with the piston top through the connecting rod, a spring is arranged in the piston body part, one end of the spring is movably connected with the piston connecting rod through a movable panel, and the other end of the spring is fixed on a fixed panel at the lower end of the piston body part. According to the sampling method, the suction head approaches a brain slice, and the piston top is pressed slightly and then loosened slowly to suck the brain slice. The brain slice sampler which is compact in structure, simple to operate, stable and reliable is provided for related professional and scientific research and experiments, the brain slice transfer efficiency is increased and damage to the brain slice is reduced to the utmost extent for nerve electrophysiology experiment researchers, and the survival rate of brain slice tissue is increased.
Description
Technical field
The present invention relates to a kind of brain sheet electro physiology experimental provision, be specifically related to a kind of spring brain sheet sampler and sampling method thereof.
Background technology
At present in institution of higher learning and scientific research institution, the electro physiology experimental study of many relevant specialities all will carry out remembering, sleep, the experiment such as thinking activities, brain function, especially the relevant speciality such as medicinal pharmacology, needs to carry out a large amount of experimental studies relevant to nervous system activity.The brain sheet sampling experimental provision (as shown in Figure of description 2) generally adopted now, generally adopt common dropper, because caliber is less than normal, very easily cause brain sheet torsional deformation, also there is people directly to use little spoon, all can be easy to damage brain sheet during operation and cause the failure of an experiment.
Summary of the invention
Goal of the invention: the object of the invention is to solve deficiency of the prior art, provides a kind of rational in infrastructure, functional and the brain sheet sampler of stable operation and sampling method thereof.
Technical scheme: a kind of spring brain sheet sampler of the present invention, comprises glass suction nozzle, bending pipe and controllable piston, described glass suction nozzle is circular behind front; One end of described bending pipe is connected with described controllable piston parts by connector, the other end of described bending pipe is connected with glass suction nozzle, described controllable piston parts comprise top, connecting link and piston body part, the upper end of described piston body part is connected with described top movable by connecting link, also spring is provided with in described piston body part, described spring one end is flexibly connected with described connecting link by drop front, and the other end of described spring is fixed on the fixed panel of piston body part lower end.
As optimization, the material of described drop front is snappy rubber.
As optimization, the material of described piston body part is rigid plastic.
As optimization, described bending pipe is hard micro-bullet plastics bending pipe, and the radian of bending pipe is 110-120 degree, and bore is 3 millimeters.
As optimization, described glass suction nozzle is the glass suction nozzle of circle behind front, and comprises two cover glass suction nozzles, and the large length of side is 6 millimeters, the little length of side is 4 millimeters.
The invention also discloses a kind of sampling method of spring brain sheet sampler, comprise the steps:
(1) take out the good glass suction nozzle of clean dry and bending pipe, the round nose of bending pipe one end and glass suction nozzle is connected;
(2) the bending pipe other end with glass suction nozzle be connected with adjusting piston connector and adjust to optimum orientation;
(3) with hand fixed piston body, thumb is close to the top of piston;
(4) this brain sheet sampler moved to above the brain sheet container filling and just cut, slighter compress thumb, extend into square suction nozzle near the brain sheet wanting to get, slowly lifts thumb, makes target brain sheet be drawn in bending pipe;
(5) again this brain sheet sampler is transferred under preprepared fills the liquid level of nutrient solution brain sheet couveuse, then press thumb gently, got brain sheet is put into brain sheet couveuse, completes the sampling of brain sheet.
Beneficial effect: the present invention has following beneficial effect:
(1) adopt circular suction nozzle behind glass front, avoid damaging brain sheet when sampling and being convenient to connect;
(2) bending pipe is convenient to the angle regulating suction nozzle, makes operation accurately more convenient;
(3) Microspring operating rod makes sampling stablize more flexibly, greatly can improve the security of brain sheet;
(4) brain sheet sampling operation process stabilization, accurately, serviceability is good.
Visible the present invention is that relevant speciality scientific experiment provides compact conformation, simple to operate and reliable and stable brain sheet sampler, the efficiency that improve the transfer of brain sheet for Electrophysiology experimental study personnel and the damage decreased to greatest extent brain sheet, improve the survival rate of brain sheet tissue.
Accompanying drawing explanation
Fig. 1 is the structural representation of one embodiment of the present of invention.
Fig. 2 is sampler structural drawing of the prior art.
Embodiment
A kind of spring brain sheet sampler as shown in Figure 1, comprise glass suction nozzle 9, bending pipe 8 and controllable piston parts, one end of bending pipe 8 is connected with described controllable piston parts by connector 7, and the other end of described bending pipe 8 is connected with the glass suction nozzle 9 of circle behind front, described controllable piston parts comprise top 1, connecting link 2 and piston body part 3, the upper end of described piston body part 3 is flexibly connected with described top 1 by connecting link 2, spring 5 is also provided with in described piston body part 3, described spring 5 one end is flexibly connected with described connecting link 2 by drop front 4, the other end of described spring 5 is fixed on the fixed panel 6 bottom piston body part 3, wherein the material of drop front 4 is snappy rubber, the material of piston body part 3 is rigid plastic, bending pipe 8 is hard micro-bullet plastics bending pipe, the radian of bending pipe 8 is 110-120 degree, bore is 3 millimeters.Glass suction nozzle 9 is the glass suction nozzle of circle behind front, and comprises two cover glass suction nozzles, and the large length of side is 6 millimeters, the little length of side is 4 millimeters.
The sampling method that spring brain sheet sampler of the present invention is concrete comprises the steps:
(1) take out the good glass suction nozzle of clean dry and bending pipe, the round nose of bending pipe one end and glass suction nozzle is connected;
(2) the bending pipe other end with glass suction nozzle be connected with adjusting piston connector and adjust to optimum orientation;
(2) with hand fixed piston body, thumb is close to the top of piston;
(3) this brain sheet sampler moved to above the brain sheet container filling and just cut, slighter compress thumb, extend into square suction nozzle near the brain sheet wanting to get, slowly lifts thumb, makes target brain sheet be drawn in glass suction nozzle;
(4) again this brain sheet sampler is transferred under preprepared fills the liquid level of nutrient solution brain sheet couveuse, then press thumb gently, got brain sheet is put into brain sheet couveuse, completes the sampling of brain sheet.
Piston portion in this device is the important step of this experimental implementation, can strengthen the stability of experimental implementation.The head of brain sheet sampler adopts square head portion, reduces the damage to brain sheet to a great extent; Bending pipe can regulate the optimum orientation getting brain sheet, the application of controlled control lever, completely avoid because of rocking and the uncertain faulty operation such as vibrations during mobile brain sheet.This apparatus structure is reliable and stable, and operation is simple for specific experiment, easy to clean, and the laboratory of applicable each colleges and universities, scientific research institution's related discipline professional domain widely uses, and can significantly improve the success ratio of experiment.
The invention of this experimental provision is rational in infrastructure, is mainly reflected in following several respects: (1) adopt glass square suction nozzle, avoids the damage brain sheet when sampling; (2) bending pipe is convenient to control suction nozzle direction; (3) Microspring operating rod makes more flexible accurate, the safety and stability of sampling; (4) brain sheet sampling work is functional.
The present invention is that relevant speciality scientific experiment provides compact conformation, simple to operate and reliable and stable brain sheet sampler, the efficiency that improve the transfer of brain sheet for Electrophysiology experimental study personnel and the damage decreased to greatest extent brain sheet, improve the survival rate of brain sheet tissue.
Technology contents of the present invention and technical characteristic have disclosed as above; but those of ordinary skill in the art still may do all replacement and the modification that do not deviate from spirit of the present invention based on announcement of the present invention; therefore; scope should be not limited to the content that embodiment discloses; and various do not deviate from replacement of the present invention and modification should be comprised, and contained by present patent application claim.
Claims (6)
1. a spring brain sheet sampler, it is characterized in that: comprise glass suction nozzle, bending pipe and controllable piston, one end of described bending pipe is connected with described controllable piston parts by connector, and the other end of described bending pipe is connected with glass suction nozzle, and described glass suction nozzle is circular behind front; Described controllable piston parts comprise top, connecting link and piston body part, the upper end of described piston body part is connected with described top movable by connecting link, also spring is provided with in described piston body part, described spring one end is flexibly connected with described connecting link by drop front, and the other end of described spring is fixed on the fixed panel of piston body part lower end.
2. a kind of spring brain sheet sampler according to claim 1, is characterized in that: the material of described drop front is snappy rubber.
3. a kind of spring brain sheet sampler according to claim 1, is characterized in that: the material of described piston body part is rigid plastic.
4. a kind of spring brain sheet sampler according to claim 1, is characterized in that: the material of described bending pipe is hard micro-bullet plastics bending pipe, and the radian of bending pipe is 110-120 degree, and bore is 3 millimeters.
5. a kind of spring brain sheet sampler according to claim 1, is characterized in that: described glass suction nozzle is the glass suction nozzle of circle behind front, and comprises two cover glass suction nozzles, and the large length of side is 6 millimeters, the little length of side is 4 millimeters.
6. a sampling method for the spring brain sheet sampler as described in any one of claim 1-5, its feature comprises following sampling procedure:
(1) take out the good glass suction nozzle of clean dry and bending pipe, the round nose of bending pipe one end and glass suction nozzle is connected;
(2) the bending pipe other end with glass suction nozzle be connected with adjusting piston connector and adjust to optimum orientation;
(3) with hand fixed piston body, thumb is close to the top of piston;
(4) this brain sheet sampler moved to above the brain sheet container filling and just cut, slighter compress thumb, extend into square suction nozzle near the brain sheet wanting to get, slowly lifts thumb, makes target brain sheet be drawn in square glass suction nozzle;
(5) again this brain sheet sampler is transferred under preprepared fills the liquid level of nutrient solution brain sheet couveuse, then press thumb gently, got brain sheet is put into brain sheet couveuse, completes the sampling of brain sheet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410546055.2A CN104266860B (en) | 2014-10-16 | 2014-10-16 | A kind of spring brain piece sampler and its sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410546055.2A CN104266860B (en) | 2014-10-16 | 2014-10-16 | A kind of spring brain piece sampler and its sampling method |
Publications (2)
| Publication Number | Publication Date |
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| CN104266860A true CN104266860A (en) | 2015-01-07 |
| CN104266860B CN104266860B (en) | 2017-06-16 |
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| CN201410546055.2A Active CN104266860B (en) | 2014-10-16 | 2014-10-16 | A kind of spring brain piece sampler and its sampling method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107020166A (en) * | 2017-04-07 | 2017-08-08 | 周肇梅 | A kind of use for laboratory liquid getting device |
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| US20140114210A1 (en) * | 2012-10-24 | 2014-04-24 | William Zinnanti | Biopsy device with automatic aspiration |
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| CN107020166A (en) * | 2017-04-07 | 2017-08-08 | 周肇梅 | A kind of use for laboratory liquid getting device |
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|---|---|
| CN104266860B (en) | 2017-06-16 |
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Effective date of registration: 20210621 Address after: 226019 No.205, building 6, Nantong University, No.9, Siyuan Road, Nantong City, Jiangsu Province Patentee after: Center for technology transfer, Nantong University Address before: 226000 No. 9 Siyuan Road, Chongchuan District, Nantong City, Jiangsu Province Patentee before: NANTONG University |
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