CN104323868A - Micro-infusion method and device thereof based on epididymis duct intracavity environment experiment - Google Patents
Micro-infusion method and device thereof based on epididymis duct intracavity environment experiment Download PDFInfo
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- CN104323868A CN104323868A CN201410618576.4A CN201410618576A CN104323868A CN 104323868 A CN104323868 A CN 104323868A CN 201410618576 A CN201410618576 A CN 201410618576A CN 104323868 A CN104323868 A CN 104323868A
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- micro
- duct
- perfusion
- graft
- epididymis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/43—Detecting, measuring or recording for evaluating the reproductive systems
- A61B5/4375—Detecting, measuring or recording for evaluating the reproductive systems for evaluating the male reproductive system
- A61B5/4387—Testicles, seminal vesicles or sperm ducts evaluation
Abstract
The invention relates to a micro-infusion method and a device thereof based on epididymis duct intracavity environment experiment. In international reports, the live micro-infusion method on the function research of epididymis ducts adopts the steps of by utilizing a dissecting microscope, separating the epididymis duct from the epididymis duct tail part, forming an incision, inserting a catheter, fixing by plasticine, separating an accompanying blood vessel near an spermaduct, and inserting a catheter, so the difficulty in intubation operation is larger. The method comprises the following steps of separating the epididymis duct from the epididymis duct tail part; using a glass micro-infusion tube, drawing and thinning a head part, and connecting the tail part with a silicone tube, a syringe and a micro injection pump; directly inserting a micro-infusion pipe head part into the epididymis duct, dripping a tissue glue for fixing, and filling interference materials; directly inserting the silicone tube without separation of the accompanying blood vessel near the spermaduct, and fixing by the tissue glue; finally, backflushing the spermaduct, and collecting epididymis duct liquid at the opening of the injection part of the epididymis duct. The method and the device have the advantages that the micro-infusion operation under the live environment is optimized, the operation is simple, the implementation is easy, the required material is purchased domestically, the experiment cycle is greatly shortened, and the experiment efficiency is improved.
Description
Technical field
the invention belongs to epididymal duct intracavity environmental experiment field, be specifically related to a kind of Micro-perfusion in Graft After method based on epididymal duct intracavity environmental experiment and device thereof.
Background technology
At present, the domestic research for reproduction of male animal system epididymal function is studied mostly under the ex vivo situation such as tissue sampling, cell culture, and rarely have report about the research under live body environment, only have the research group of minority to adopt Micro-perfusion in Graft After method under live body environment to carry out the research of epididymis correlation function in the world.They are separated epididymal duct from cauda epididymis and make otch and intubate, and plasticine is fixed, and then makes another opening and intubate at deferent duct near-end, inject different material, the change of research epididymal function with pouring in down a chimney note normal direction cauda epididymis.But we find to there is following problem in implementation process: 1. experimental implementation difficulty is large, because epididymal duct caliber is thin, tube wall is thin, operate slightly unskilled epididymal duct tractive will being broken, even if constantly, owing to being filled with seminal fluid in epididymal duct, after doing otch, seminal fluid is constantly gushed out, and affects intubate; 2. pair apparatus, material requirements are high, and intubate diameter 200 μm of pvc pipes used are not sold at home, and the ligature of domestic shears tweezers, plasticine and fixing deferent duct intubate is all difficult to meet operation requirements.3., during deferent duct intubate of being expert at, a critical operations needs to be separated [Dan exactly, but [Dan is very thin, branch is more and share one deck fascia with deferent duct, and gap is small between the two, is separated comparatively difficulty, be easy to injured blood vessel, once hemorrhage, will experimental implementation be affected; 4. when deferent duct intubate is fixed, it all has higher requirements to ligature thickness, material, uses domestic ligature to be easy to cause intubate slippage, or ligature cutting deferent duct tube wall.Therefore, successfully this scheme will be used for experiment, operator needs expensive time and efforts to practise and selection material, and the cycle of coming into operation is longer.
Summary of the invention
The object of this invention is to provide a kind of Micro-perfusion in Graft After method based on epididymal duct intracavity environmental experiment and device thereof, solve epididymal duct and difficult, the fixing difficult problem of deferent duct intubate in prior art.
The technical solution adopted in the present invention is:
Based on the Micro-perfusion in Graft After method of epididymal duct intracavity environmental experiment, it is characterized in that:
Realized by following steps:
Step one: carry out intraperitoneal anesthesia to rat, exposes epididymis in scrotum paramedian incision;
Step 2: under anatomic microscope, removes 1mm at cauda epididymis fine forceps
2connective tissue, the epididymal duct that free ring-type is spiraled;
Step 3: the Micro-perfusion in Graft After pipe getting glass material, internal diameter 0.5 mm, external diameter 0.8 mm, head pulls into fine needle by trombone slide instrument, needle tip external diameter is less than 0.2 mm, Micro-perfusion in Graft After pipe afterbody connects the silica gel tube that internal diameter is 0.8 mm, and the silica gel tube other end connects the syringe of 1 ml, and syringe connects micro-injection pump;
Step 4: the syringe needle of Micro-perfusion in Graft After tube head is inserted epididymal duct, fixes Micro-perfusion in Graft After pipe in catheter position Dian Fu tissue glue, micro-injection pump injects with the speed of 1-2 μ l/min intervenes material;
Step 5: in the silica gel tube intubate of deferent duct near-end with external diameter 300 μm after 30 min, fix intubate in catheter position Dian Fu tissue glue;
Step 6: epididymal duct is left an osculum from injection site, the epididymal fluid of cauda epididymis to be gone out from deferent duct with the speed PBS reverse flushing of 45 μ l/min and is collected by micro-injection pump.
The Micro-perfusion in Graft After device used in the described Micro-perfusion in Graft After method based on epididymal duct intracavity environmental experiment, is characterized in that:
Be provided with the Micro-perfusion in Graft After pipe of glass material, internal diameter is 0.5mm, and external diameter is 0.8mm;
Described Micro-perfusion in Graft After tube head pulls into fine needle by trombone slide instrument, and needle tip external diameter is less than 0.2 mm;
Described Micro-perfusion in Graft After pipe afterbody is connected with the silica gel tube that internal diameter is 0.8mm.
Described Micro-perfusion in Graft After cannula location point covers tissue glue.
The described silica gel tube other end connects the syringe of 1ml, and syringe connects micro-injection pump.
The present invention has the following advantages:
1, material is easy to get:
General glass capillary can meet, and is 0.5 mm by internal diameter, and external diameter is that glass capillary one end of 0.8 mm pulls into thinner pin on trombone slide instrument; Fix the surgical operation glue that the tissue glue of intubate is general.The plasticine difficulty solving external conventional fixing epididymal duct intubate is bought, domestic plasticine does not reach again the problem of requirement, also solves the problem that domestic ligature is difficult to fixing deferent duct intubate simultaneously.
2, simple to operate:
On the one hand, compared to external pvc pipe, this catheter needle tip is comparatively hard, not be used on epididymal duct and does otch, can direct cannulation, thus avoids seminal fluid when doing otch and overflow and the problem of the softer difficulty operation of pvc pipe.On the other hand, tissue glue is selected to fix intubate, simple, in intubate Chu Dijidi tissue glue, intubate and tissue can be fixed instantaneously, both avoided plasticine to fix epididymal duct intubate external force and cross the situation that senior general's epididymal duct closes, turn avoid to cut deferent duct and intubate slippage when fixing with ligature may.Also need not be separated the blood vessel of deferent duct companion row in addition, directly do a kerf at deferent duct place, avoid being separated the operating influence that improper angiorrhexis causes.
Accompanying drawing explanation
Fig. 1 is Micro-perfusion in Graft After structure drawing of device.
In figure, 1-Micro-perfusion in Graft After pipe, 2-syringe needle, 3-tissue glue, 4-silica gel tube.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention will be described in detail.
The Micro-perfusion in Graft After method based on epididymal duct intracavity environmental experiment that the present invention relates to, studying at body for buck epididymal function, be particularly useful for the research of epididymal function and epididymis tube chamber micro-environmental variation, after utilizing special instruments and equipment to apply external source intervention to epididymis tube chamber, epididymal fluid can be collected and obtain the change information after intervening, also observable normal epididymis luminal environment and epithelial physiological change, be a kind ofly to obtain as the method for the information of intermediate object program from live body, specifically realized by following steps:
Step one: carry out intraperitoneal anesthesia to rat, exposes epididymis in scrotum paramedian incision;
Step 2: under anatomic microscope, removes 1mm at cauda epididymis fine forceps
2connective tissue, the epididymal duct that free ring-type is spiraled;
Step 3: the Micro-perfusion in Graft After pipe getting glass material, internal diameter 0.5 mm, external diameter 0.8 mm, head pulls into fine needle by trombone slide instrument, needle tip external diameter is less than 0.2 mm, Micro-perfusion in Graft After pipe afterbody connects the silica gel tube that internal diameter is 0.8 mm, and the silica gel tube other end connects the syringe of 1 ml, and syringe connects micro-injection pump;
Step 4: the syringe needle of Micro-perfusion in Graft After tube head is inserted epididymal duct, fixes Micro-perfusion in Graft After pipe in catheter position Dian Fu tissue glue, micro-injection pump injects with the speed of 1-2 μ l/min intervenes material;
Step 5: in the silica gel tube intubate of deferent duct near-end with external diameter 300 μm after 30 min, fix intubate in catheter position Dian Fu tissue glue;
Step 6: epididymal duct is left an osculum from injection site, the epididymal fluid of cauda epididymis to be gone out from deferent duct with the speed PBS reverse flushing of 45 μ l/min and is collected by micro-injection pump.
Detect the change of epididymal fluid pH value and component of organization, or the physiological change of detection epididymal epithelial cells of drawing materials.
The Micro-perfusion in Graft After device related in said method, prior art basis obtains by improving, do not adopt and on epididymal duct, do otch insert pvc pipe, but the Micro-perfusion in Graft After pipe 1 of glass material is set, Micro-perfusion in Graft After pipe 1 head is pulled into fine needle 2 by trombone slide instrument, Micro-perfusion in Graft After pipe 1 afterbody connects silica gel tube 4, and silica gel tube 4 other end connects the syringe (1ml) of 1ml, and syringe connects micro-injection pump.Micro-perfusion in Graft After pipe 1 catheter position Dian Fu tissue glue 3.The internal diameter of described Micro-perfusion in Graft After pipe 1 is 0.5mm, and external diameter is 0.8mm, and the external diameter of head syringe needle 2 is less than 0.2 mm, and silica gel tube 4 internal diameter is 0.8mm.The use of this equipment avoids being separated of deferent duct and [Dan, can direct cannulation, fixes and fixes with tissue glue, enormously simplify experimental procedure, reducing experiment difficulty, effectively can improve conventional efficient without ligature.
Content of the present invention is not limited to cited by embodiment, and the conversion of those of ordinary skill in the art by reading description of the present invention to any equivalence that technical solution of the present invention is taked, is claim of the present invention and contains.
Claims (4)
1., based on the Micro-perfusion in Graft After method of epididymal duct intracavity environmental experiment, it is characterized in that:
Realized by following steps:
Step one: carry out intraperitoneal anesthesia to rat, exposes epididymis in scrotum paramedian incision;
Step 2: under anatomic microscope, removes 1mm at cauda epididymis fine forceps
2connective tissue, the epididymal duct that free ring-type is spiraled;
Step 3: the Micro-perfusion in Graft After pipe getting glass material, internal diameter 0.5 mm, external diameter 0.8 mm, head pulls into fine needle by trombone slide instrument, needle tip external diameter is less than 0.2 mm, Micro-perfusion in Graft After pipe afterbody connects the silica gel tube that internal diameter is 0.8 mm, and the silica gel tube other end connects the syringe of 1 ml, and syringe connects micro-injection pump;
Step 4: the syringe needle of Micro-perfusion in Graft After tube head is inserted epididymal duct, fixes Micro-perfusion in Graft After pipe in catheter position Dian Fu tissue glue, micro-injection pump injects with the speed of 1-2 μ l/min intervenes material;
Step 5: in the silica gel tube intubate of deferent duct near-end with external diameter 300 μm after 30 min, fix intubate in catheter position Dian Fu tissue glue;
Step 6: epididymal duct is left an osculum from injection site, the epididymal fluid of cauda epididymis to be gone out from deferent duct with the speed PBS reverse flushing of 45 μ l/min and is collected by micro-injection pump.
2. the Micro-perfusion in Graft After device used in the Micro-perfusion in Graft After method based on epididymal duct intracavity environmental experiment according to claim 1, is characterized in that:
Be provided with the Micro-perfusion in Graft After pipe (1) of glass material, internal diameter is 0.5mm, and external diameter is 0.8mm;
Described Micro-perfusion in Graft After pipe (1) head pulls into fine needle (2) by trombone slide instrument, and syringe needle (2) tip outer diameter is less than 0.2 mm;
Described Micro-perfusion in Graft After pipe (1) afterbody is connected with the silica gel tube (4) that internal diameter is 0.8mm.
3. a kind of rat epididymis Micro-perfusion in Graft After device according to claim 2, is characterized in that:
Described Micro-perfusion in Graft After pipe (1) catheter position Dian Fu tissue glue (3).
4. the Micro-perfusion in Graft After device used in the Micro-perfusion in Graft After method based on epididymal duct intracavity environmental experiment according to claim 3, is characterized in that:
Described silica gel tube (4) other end connects the syringe of 1ml, and syringe connects micro-injection pump.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410618576.4A CN104323868A (en) | 2014-11-06 | 2014-11-06 | Micro-infusion method and device thereof based on epididymis duct intracavity environment experiment |
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| CN201410618576.4A CN104323868A (en) | 2014-11-06 | 2014-11-06 | Micro-infusion method and device thereof based on epididymis duct intracavity environment experiment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106923929A (en) * | 2017-02-24 | 2017-07-07 | 西北农林科技大学 | A kind of mammary gland of mouse injection needle and its application |
| CN113171806A (en) * | 2021-03-04 | 2021-07-27 | 深圳大学 | Disposable perfusion specimen intubation tube |
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| US4451254A (en) * | 1982-03-15 | 1984-05-29 | Eli Lilly And Company | Implant system |
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| WO2001052766A2 (en) * | 2000-01-21 | 2001-07-26 | Novus International, Inc. | Inoculation apparatus and method |
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| US4451254A (en) * | 1982-03-15 | 1984-05-29 | Eli Lilly And Company | Implant system |
| US5199952A (en) * | 1991-04-09 | 1993-04-06 | Morf, Inc. | Bird injection system |
| WO2001052766A2 (en) * | 2000-01-21 | 2001-07-26 | Novus International, Inc. | Inoculation apparatus and method |
| CN201299855Y (en) * | 2008-10-22 | 2009-09-02 | 中国人民解放军第二军医大学 | Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis |
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| CN102784012A (en) * | 2012-03-16 | 2012-11-21 | 中山大学 | Blood brain barrier pharmacokinefic continuous dosing system and detecting system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106923929A (en) * | 2017-02-24 | 2017-07-07 | 西北农林科技大学 | A kind of mammary gland of mouse injection needle and its application |
| CN113171806A (en) * | 2021-03-04 | 2021-07-27 | 深圳大学 | Disposable perfusion specimen intubation tube |
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