CN1074709A - A kind of making method of zymoplasm - Google Patents
A kind of making method of zymoplasm Download PDFInfo
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- CN1074709A CN1074709A CN 92112188 CN92112188A CN1074709A CN 1074709 A CN1074709 A CN 1074709A CN 92112188 CN92112188 CN 92112188 CN 92112188 A CN92112188 A CN 92112188A CN 1074709 A CN1074709 A CN 1074709A
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- zymoplasm
- damping fluid
- making method
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- throw out
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Abstract
A kind of making method of zymoplasm, it is based on animal blood, adds antithrombotics, and last chromatography column chromatography, damping fluid and adjusting pH value etc. make protein molecule " dehydration ", and step such as easy precipitation makes a kind of fast-acting nemostatic medicine zymoplasm when iso-electric point.The present invention has following advantage: technical process is simple, invest little, cost is low, the time is short, purity is high, and is active high, benefit or the like.
Description
The present invention relates to a kind of hemostatic drug, particularly to the various hemorrhage making method that can both reach a kind of zymoplasm of fast-acting nemostatic.
At present, hemorrhage what adopt is that bleeding stopping period is long with the hemostasis of conventional hemostatic drug to various clinically, has again to pay to act on, and particularly to hemorrhage due to the hemopathy, haemostatic effect is poorer; And the use zymoplasm, bleeding stopping period is short, and effect is rapidly, and is evident in efficacy, easy to use, has no side effect.Zymoplasm directly acts on blood coagulation, makes Fibrinogen change fibrin polymer rapidly into, comprises that simultaneously red corpuscle, white corpuscle, thrombocyte, blood plasma constitute blood clot, stops up the blutpunkte, reaches the hemostasis purpose.
Cohn has set up cold ethanol method in 1940, this method master usefulness is the change (five change systems) by five factors, and plasma proteins is separated.European patent 56629, German Patent 2902657 and United States Patent (USP) 92626 are introduced respectively is to be preparation with barium sulfate, aluminium hydroxide, calcium phosphate, sodium phosphate, polyglycol and hydroxy alkanoic acid, carry out absorption, separation with calcium ion, reach the purpose of the separation of plasma proteins and purification, adopt the weak point of the zymoplasm that above-mentioned several method manufactures to be: the technical process complexity, investment is big, the cost height, and the time is long, purity is low, active low, get profit few.
The object of the invention is to avoid above-mentioned the deficiencies in the prior art part, provides a kind of technical process simple, invests for a short time, and cost is low, and the time is short, and the purity height is active high, the making method of fast-acting nemostatic medicine-zymoplasm of benefit.
Purpose of the present invention can reach by following measure: a kind of making method of zymoplasm is characterized in that: after in animal blood, adding antithrombotics, add ethanol and damping fluid again, and the PH adjustable value, centrifugation has throw out to produce (being thrombogen); The throw out that is dissolved in water, last chromatography column chromatography, it is centrifugal that effluent liquid is regulated pH value with damping fluid, has throw out to produce; The throw out that is dissolved in water again, normal temperature activation were down transferred pH value with damping fluid more than one hour, last chromatography column chromatography, and elutriant is zymoplasm.
Purpose of the present invention can also reach by following measure: after adding ethanol or damping fluid, transferring pH value is 5.3 o'clock, can from separation; Last heparin-agarose chromatography column chromatography, effluent liquid with damping fluid transfer pH value be 4.4 o'clock centrifugal; Normal temperature is the solution of activation more than 3 hours down, uses CaHPO
4Transfer H7.2, last dextran G-25 chromatography.
The present invention is described in further detail below with reference to (accompanying drawing) embodiment:
Get animal blood (as pig blood or ox blood) liquid, add the anti-freezing of antithrombotics Trisodium Citrate; The dehydrated alcohol of adding 10% in the blood plasma after the centrifugation, centrifugal when transferring PH5.3 with damping fluid HAC10%, throw out produces, and is thrombogen; The thrombogen precipitation adds water dissolution, last heparin-agarose chromatography column, and the control certain flow, centrifugal after the effusive liquid of chromatography column is transferred PH4.4 with HAC, be precipitated as purified and treat proenzyme with fixed attention; Precipitation adds 10 times with the upper volume water dissolution, and water at normal temperature bathization 1 hour is used CaHPO
4Transfer PH7.2, add CaCl
2, go up the G-25 chromatography then, effluent liquid is refining zymoplasm, and is aseptic subpackaged through degerming, is finished product after the freeze-drying.
The present invention is prior art relatively, and have following advantage: technological process is simple, invest littlely, and cost is low, and the time is short, the purity height, and active height gets benefit etc.
Claims (4)
1, a kind of making method of zymoplasm is characterized in that:
A. after in animal blood, adding antithrombotics, add ethanol and damping fluid again, the PH adjustable value, centrifugation has throw out to produce (being thrombogen);
B. the throw out that is dissolved in water, last chromatography column chromatography, it is centrifugal that effluent liquid is regulated pH value with damping fluid, has throw out to produce;
C. the throw out that is dissolved in water again, normal temperature activation were down transferred pH value with damping fluid more than one hour, last chromatography column chromatography, and elutriant is zymoplasm.
2, the making method of zymoplasm according to claim 1 is characterized in that: after adding ethanol or damping fluid, transferring pH value is 5.3 o'clock, i.e. centrifugation.
3, the making method of zymoplasm according to claim 1 is characterized in that: go up heparin-agarose chromatography column chromatography, effluent liquid with damping fluid transfer pH value be 4.4 o'clock centrifugal.
4, the making method of zymoplasm according to claim 1 is characterized in that: normal temperature is the solution of activation more than 1 hour down, uses CaHPO
4Transfer H7.2, last dextran G-25 chromatography.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92112188 CN1074709A (en) | 1992-11-26 | 1992-11-26 | A kind of making method of zymoplasm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92112188 CN1074709A (en) | 1992-11-26 | 1992-11-26 | A kind of making method of zymoplasm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1074709A true CN1074709A (en) | 1993-07-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92112188 Pending CN1074709A (en) | 1992-11-26 | 1992-11-26 | A kind of making method of zymoplasm |
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| CN (1) | CN1074709A (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5585007A (en) * | 1994-12-07 | 1996-12-17 | Plasmaseal Corporation | Plasma concentrate and tissue sealant methods and apparatuses for making concentrated plasma and/or tissue sealant |
| CN1063789C (en) * | 1996-04-06 | 2001-03-28 | 济南军区生物制品药物研究所 | method for extracting and separating thrombin of beef |
| CN1294257C (en) * | 2004-09-15 | 2007-01-10 | 黄耀江 | Process for preparing thrombase |
| US8801586B2 (en) | 2008-02-29 | 2014-08-12 | Biomet Biologics, Llc | System and process for separating a material |
| CN105821025A (en) * | 2016-04-06 | 2016-08-03 | 浙江丰安生物制药有限公司 | Extraction method of thrombin |
| US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US9649579B2 (en) | 2007-04-12 | 2017-05-16 | Hanuman Llc | Buoy suspension fractionation system |
| US9701728B2 (en) | 2008-02-27 | 2017-07-11 | Biomet Biologics, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
| US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
| US9897589B2 (en) | 2002-05-24 | 2018-02-20 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
| US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
| US10183042B2 (en) | 2002-05-24 | 2019-01-22 | Biomet Manufacturing, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
| US10576130B2 (en) | 2013-03-15 | 2020-03-03 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
| US11957733B2 (en) | 2019-10-28 | 2024-04-16 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
-
1992
- 1992-11-26 CN CN 92112188 patent/CN1074709A/en active Pending
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6214338B1 (en) | 1994-12-07 | 2001-04-10 | Plasmaseal Llc | Plasma concentrate and method of processing blood for same |
| US5585007A (en) * | 1994-12-07 | 1996-12-17 | Plasmaseal Corporation | Plasma concentrate and tissue sealant methods and apparatuses for making concentrated plasma and/or tissue sealant |
| CN1063789C (en) * | 1996-04-06 | 2001-03-28 | 济南军区生物制品药物研究所 | method for extracting and separating thrombin of beef |
| US10183042B2 (en) | 2002-05-24 | 2019-01-22 | Biomet Manufacturing, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US9897589B2 (en) | 2002-05-24 | 2018-02-20 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US10393728B2 (en) | 2002-05-24 | 2019-08-27 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| CN1294257C (en) * | 2004-09-15 | 2007-01-10 | 黄耀江 | Process for preparing thrombase |
| US9649579B2 (en) | 2007-04-12 | 2017-05-16 | Hanuman Llc | Buoy suspension fractionation system |
| US10400017B2 (en) | 2008-02-27 | 2019-09-03 | Biomet Biologics, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
| US9701728B2 (en) | 2008-02-27 | 2017-07-11 | Biomet Biologics, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
| US11725031B2 (en) | 2008-02-27 | 2023-08-15 | Biomet Manufacturing, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
| US9719063B2 (en) | 2008-02-29 | 2017-08-01 | Biomet Biologics, Llc | System and process for separating a material |
| US8801586B2 (en) | 2008-02-29 | 2014-08-12 | Biomet Biologics, Llc | System and process for separating a material |
| US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
| US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
| US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
| US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
| US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
| US10441634B2 (en) | 2013-03-15 | 2019-10-15 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
| US10576130B2 (en) | 2013-03-15 | 2020-03-03 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
| US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
| CN105821025B (en) * | 2016-04-06 | 2019-06-28 | 浙江丰安生物制药有限公司 | A kind of extracting method of fibrin ferment |
| CN105821025A (en) * | 2016-04-06 | 2016-08-03 | 浙江丰安生物制药有限公司 | Extraction method of thrombin |
| US11957733B2 (en) | 2019-10-28 | 2024-04-16 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
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