CN1295494C - Integrated minisize optical analyser - Google Patents
Integrated minisize optical analyser Download PDFInfo
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- CN1295494C CN1295494C CNB2004100134182A CN200410013418A CN1295494C CN 1295494 C CN1295494 C CN 1295494C CN B2004100134182 A CNB2004100134182 A CN B2004100134182A CN 200410013418 A CN200410013418 A CN 200410013418A CN 1295494 C CN1295494 C CN 1295494C
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Abstract
The present invention discloses an integrated miniature optical analyzer. The integrated miniature optical analyzer comprises a light path part, a monolithic computer, an auxiliary circuit part and an input-output part, wherein the monolithic computer and the auxiliary circuit part are used for calculation and control; the input-output part is used for inputting and outputting information. The integrated miniature optical analyzer is characterized in that the light path part comprises a light source voltage stabilizing control circuit, a first monochromatic light source assembly, a second monochromatic light source assembly, sample tanks, an optical splitter, photoelectric detectors, a photoelectric sensor array and a signal modulating circuit; the first monochromatic light source assembly, the sample tank and the photoelectric detector are successively positioned on a first light path, the second monochromatic light source assembly, a sample tank, the optical splitter and a photoelectric detector are successively positioned on a second light path, and the first light path and the second light path have an included angle of 90 degrees. The present invention integrates a plurality of optical detection techniques, greatly reduces the size and the power consumption of analyzers, realizes the miniaturization of instruments, and can be used for simultaneous in-situ rapid determination of multiple components in environmental samples, food samples and other complicated samples.
Description
Technical field
The invention belongs to the optical method analysis and detection technology, be specifically related to a kind of integrated micro optical analyser.
Technical background
Because the abuse of environmental pollution, agricultural chemicals, growth hormone and food additives, the people's health is produced greatly harm, so environment and food-safety problem are subjected to showing great attention to of the country and people masses.The test event of environmental analysis and food analysis is a lot, their measuring principle has nothing in common with each other, as optical analysis (absorption photometric, fluorescence, luminesceence analysis etc.), stratographic analysis etc., wherein Optical Analysis Method becomes the most widely used analytical approach because of advantages such as its instrument are simple, easy and simple to handle.In food and the environmental analysis, various optical analysis methods are used widely.The exploitation of microminiaturized portable intelligent analytical instrument has also had significant progress in recent years.
But instruments such as the spectrophotometer of laboratory use at present, fluorophotometer, luminescence analyzer, because volume is big, be not easy to carry, need mains-supplied, thus be difficult to satisfy in the open air, needs field monitorings such as country fair, supermarket, supervision occasion are to the demand of the analytical instrument microminiaturized, portable, quick, easy, that multicomponent is measured simultaneously.And instrument such as spectrophotometer, fluorophotometer, luminescence analyzer, all being based on single optical analysis principle, can not realize simultaneously based on the multicomponent fast measuring of multiple smooth analysis to measure principle or obtaining of the multiple optical measurement information of same sample.Thereby the express-analysis of complex sample such as environment, food is subjected to many restrictions in using at the scene.When for example using colourity in the Their Determination by Spectrophotometry environmental sample, COD, the turbidity in the water sample can produce interference, and blank correction usually is difficult to prove effective in these occasions, and sample pretreatment also can exert an influence to measurement result.Therefore present simple function optical analyser is difficult to accurately measure the water-quality guideline such as colourity, COD of high turbidity sample.The structural principle of instruments such as spectrophotometer, fluorophotometer, luminescence analyzer has similarity, and measuring method also respectively has relative merits.Consider that from the angle of analytical applications the microminiaturized instrument of integrated multiple optical analysis principle can use different measuring principles, obtains the qualitative, quantitative information of more sample composition from complex sample, multiple tested composition is realized fast-field evaluation.This integrated micro optical analyser with optical analysis Laboratory Function has practical significance in the complex sample analysis of the on-the-spot express-analysis occasion of environment and food supervision.
Be that light source, photodiode are that microminiaturization, the portable spectrophotometer of detecting device become abroad and use general commercialization instrument (as the HACH of the U.S., gondola HANNA) at present based on LED, this quasi-instrument AC/DC, be equipped with the use of specific chemical reagent bag, can measure tens of kinds of indexs easily, be favored in China market in recent years.But this instrument also can only be realized single absorption photometric measurement function, can't realize functions such as fluorescence, luminesceence analysis.Various panoramic optical analysers such as fluorescence analyser, luminescence analyzer, scattering photometer are also arranged on the market, but all only have the single-measurement function.
Up to now, still finding no multiple optical measuring techniques such as this class set absorption photometric, scattering luminosity, fluorophotometric and luminesceence analysis at home and abroad is the report of the micro-optical analyser of one.
Summary of the invention
The objective of the invention is to overcome the deficiency of existing single-measurement function instrument, a kind of integrated micro optical analyser is provided, this instrument can be measured multiple optical indexes such as absorbance, scattered light intensity, fluorescence intensity and luminous intensity simultaneously.
A kind of integrated micro optical analyser provided by the invention, comprise light path part, single-chip microcomputer and auxiliary circuit part and input, single-chip microcomputer and auxiliary circuit partly are used for calculating and control, input is used for the input and output of information, it is characterized in that: described light path part comprises light source constant-pressure control, first sets of monochromatic light sources and second sets of monochromatic light sources, sample cell, optical splitter, photoelectric detector, photosensor arrays and signal conditioning circuit; First sets of monochromatic light sources, sample cell and photoelectric detector are positioned on first light path successively, and second sets of monochromatic light sources, sample cell, optical splitter and photoelectric detector are positioned on second light path successively, first light path and second light path degree of one-tenth angle; Light source constant-pressure control provides operating voltage according to the signal that single-chip microcomputer and auxiliary circuit partly provide for monochromatic source, and the switch of control light source; Sets of monochromatic light sources or produce monochromatic homogeneous beam and pass the testing sample that is arranged in sample cell after, a light signal part that produces shines directly on the photoelectric detector, measure total light intensity value and send into signal conditioning circuit by it, a part shines on the photosensor arrays by optical splitter, measure the light intensity value of each wavelength period and send into signal conditioning circuit, signal conditioning circuit amplifies each light intensity value shaping, export to single-chip microcomputer and auxiliary circuit is partly handled, its result sends into input; First sets of monochromatic light sources and photoelectric detector assembly, or second sets of monochromatic light sources and optical splitter, photosensor arrays assembly are used for photometric analysis of extinction; First sets of monochromatic light sources and photoelectric detector assembly, perhaps second sets of monochromatic light sources and optical splitter, photosensor arrays assembly are used for the scattering photometric analysis; First sets of monochromatic light sources and optical splitter, photosensor arrays assembly are used for fluorescence analysis; Photoelectric detector, perhaps optical splitter and photosensor arrays assembly are used for luminesceence analysis.
The present invention is directed to the present situation of optic analytical instrument, a kind of micro-optical analyser that can measure the Highgrade integration of multiple optical indexes such as absorbance, scattered light intensity, fluorescence intensity and luminous intensity simultaneously is provided.By special light path design, two groups of light sources and detecting device can make up as required, the integrated function of multiple optic analytical instruments such as spectrophotometer, fluorescence analyser, luminescence analyzer, scattered light intensity instrument on an instrument.Instrument has been abandoned bulky traditional hot light source and photomultiplier detector, use the low semiconductor light sources group of volume low power consumption and highly integrated little detecting device instead, make multiple optical detective technology be integrated in an instrument and become possibility, also reduce simultaneously the volume and the power consumption of instrument greatly, realized the microminiaturization of instrument.High-performance Single Chip Microcomputer (SCM) system that instrument is built-in can satisfy the requirement of high-speed sampling and complicated numerical evaluation.By cooperative and aggregation of data analyzing and processing, can realize fast-field evaluation multiple composition time the in the complex samples such as environment and food to multiple optical index.At home and abroad do not see as yet at present the analogous instrument report is arranged.
Description of drawings
Fig. 1 is the theory diagram of instrument hardware;
Fig. 2 is the software flow block diagram of apparatus measures part.
Fig. 3 is the optical table structural representation of instrument;
Embodiment
This instrument mainly comprises three parts: light path part 1, single-chip microcomputer and auxiliary circuit part 2 and input 3.Light path part 1 mainly is made of light source constant-pressure control 4, first sets of monochromatic light sources 5 and second sets of monochromatic light sources 6, sample cell 7, optical splitter 8, photoelectric detector 9, photosensor arrays 10 and signal conditioning circuit 11.Single-chip microcomputer and auxiliary circuit part 2 comprise single-chip microcomputer 12, multidiameter option switch 13, system monitoring circuit 14, data-storing circuit 15 etc., and input 3 comprises display device 16, input media 17 and communicating circuit 18.
Light source constant-pressure control 4 provides constant operating voltage for monochromatic source, controls the switch of light source simultaneously; First sets of monochromatic light sources 5 and second sets of monochromatic light sources 6 are made up of the semiconductor monochromatic source of a series of different wave lengths, can select the semiconductor monochromatic source combination of different wave length (ultraviolet region is to the near-infrared region) according to the demand of testing sample analytical approach.Two kinds of monochromatic sources become 90 degree homeotropic alignments (referring to accompanying drawing 3); Sample cell 7 is used to place testing sample; Optical splitter 8 is used for complex light is resolved into the monochromatic light of different wave length; Photoelectric detector 9 is used for converting total light intensity signal to electric signal; Photosensor arrays 10 is used for detecting respectively the light intensity value of different wave length, and converts thereof into electric signal; Signal conditioning circuit 11 can amplify shaping to the electric signal of output; Single-chip microcomputer 12 is computings and control core of whole instrument; Multidiameter option switch 13 can be selected suitable light source and detecting device according to the instruction of single-chip microcomputer 12; System monitoring circuit 14 is the running status of supervisory system at any time, prevents program fleet; Data-storing circuit 15 is used for data-storing at nonvolatile memory EEPROM; Display device 16 and input media 17 provide good man computer interface; Communicating circuit 18 is used for carrying out exchanges data with PC or serial printer.
After the selected as required optical index that needs to measure of user, single-chip microcomputer 12 can select suitable light source and detecting device to make up automatically.Under the control of Single Chip Microcomputer (SCM) system, light the monochromatic source of corresponding wavelength in sets of monochromatic light sources 5 or the sets of monochromatic light sources 6 by multidiameter option switch 13 and light source constant-pressure control 4, produce monochromatic light.After homogeneous beam passed the sample cell 7 that testing sample is housed, through effects such as absorption of sample and scatterings, the light signal part of generation shone directly on the photoelectric detector 9, and a part is then by shining on the photosensor arrays 10 behind the optical splitter 8.After monochromatic light shines on the sample, except transmitted light, also produce scattered light to other direction.Some sample also can produce fluorescence and chemiluminescence etc.The detected optical signal type of the detecting device of diverse location is inequality.How these light signals being gathered and handled is the problem of a more complicated, not in the scope that this patent is discussed.Photoelectric detector 9 can directly be measured total light intensity value, and optical splitter 8 and photosensor arrays 10 then can be measured the light intensity value of each different wave length section respectively.Can be after each light intensity value is amplified by the signal conditioning circuit shaping through multidiameter option switch 15 input single-chip microcomputers.Single-chip microcomputer can selectively carry out signals collecting according to needing of test, obtains measurement results such as corresponding absorbance, scattered light intensity value, fluorescent value and luminous intensity values, and can carry out further overall treatment to measurement result according to different algorithms.All measurement data are kept in the Nonvolatile data memory by data-storing circuit 15, can and gather for user inquiring.Display device 16 and input media 17 are main input-output device, and good man computer interface can be provided.By communicating circuit 18, can also realize and the data exchanging function of PC, also can directly print measurement result by external printer.Whether 14 moment of the system monitoring circuit duty of supervisory system is normal, guaranteed the reliable and stable of instrument.
The combination of several optical analysis methods of table 1
| Analytical approach | Light source | Detecting device |
| Photometric analysis of extinction photometric analysis of extinction scattering photometric analysis scattering luminosity analysis of fluorescence is analyzed the luminesceence analysis luminesceence analysis | Sets of monochromatic light sources, (A) 5 sets of monochromatic light sources, (B) 6 sets of monochromatic light sources, (A) 5 sets of monochromatic light sources, (B) 6 sets of monochromatic light sources, (A) 5-- | Photoelectric detector 9 |
The program of integrated micro optical analyser can adopt higher level lanquage C to write.Whole software can be divided into four module: parameter setting, sample measurement, data processing, system's setting.Parameter is provided with module and mainly finishes and measure relevant setting, the user as required the specified measurement wavelength, select detector set, specified measurement mode (absorption photometric, scattering luminosity, fluorescence, luminesceence analysis) etc.; The sample measurement module is the core of whole software.According to measurement value of setting of user, sample is measured, finish the function such as calculating, demonstration of monochromatic light selection, detecting device selection, sample measurement control, data acquisition and measurement result; Data processing module be responsible for finishing measurement result preservation, inquiry, printing and with the tasks such as exchanges data of PC; System is provided with module and comprises complementary functions such as system time setting, display screen contrast setting, input equipment correction.
Each part mentioned above all can adopt prior art to be realized.
Be that the utility model is further described with an example below.
We can select for use the arrowband light emitting diode LED of a plurality of specific wavelengths to make up sets of monochromatic light sources (A) 5 and sets of monochromatic light sources (A) 6 as monochromatic source, replace existing spectrophotometric thermal light source in laboratory and light source monochromator system.At present available commercialization monochromatic LED wavelength coverage is very wide, have 270,420,460,520,560,590,640,700, multi-wavelength such as 840nm can be for selecting for use, substantially contain ultraviolet, visible light and near-infrared region, can satisfy the needs of different measuring;
The micro semiconductor photoelectric detector volume of the high integration such as OPT101 of the TSL235 of TASO company, TI company is little, low in energy consumption, spectral response range is wide, stable and reliable for performance, be that the ideal of photoelectric detector 9 is selected.The ccd array detecting device product technology maturations such as IL-P3-2048 of the TCD1208 of Toshiba company, TCD1500 and DALSA company can be used as photosensor arrays 10.Optical splitter 8 can be selected grating or prism for use.
By selecting for use of photoelectric device, the optical analysis platform of the Highgrade integration that can to make up multiple optical measurement principles such as collection absorption photometric, scattering luminosity, fluorescence and luminesceence analysis be one.
As signal Processing and control core, single-chip microcomputer 12 can be selected 8 or 16 single-chip microcomputer products of enhancement mode for use, as the C8051F of Cygnal company, the MSP430 of TI company etc.; Multi-way switch 13 can be selected CD4051 for use; System monitoring circuit 14 can be selected monitoring functions such as chips such as MAX813 are realized house dog, automatically reset, voltage monitoring for use; Data-storing circuit 15 can select for use the EEPROM product of 24LC series to realize.
Display device 16 can select for use giant-screen liquid to show Chinese display LCD, and touch-screen and keyboard constitute input media 17.RS-232 serial interface chip (as MAX3233) is satisfying to be used for making up communicating circuit 18, realizes functions such as PC communication and serial print
It is little, in light weight that above-mentioned light source, detecting device, chip and each main device have a volume, can be under the 5V DC voltage operate as normal, thereby can directly adopt powered battery.These all make the miniaturized design of instrument become possibility.
The program of integrated micro optical analyser can adopt external popular Keil C single-chip microcomputer language compilation, comprises that parameter setting, sample measurement, data processing, system are provided with four module.The existing general C language characteristic of this modularized program design language, the ability that has direct control single-chip microcomputer hardware again, its basic characteristics comprise: travelling speed is fast, compile efficiency is high, have the built-in function and the floating-point operation ability of good transplantability and abundant multiple complex calculation.The software flow synoptic diagram of sample measurement module is seen Fig. 2.
Table 2 has been listed several traditional large-scale photometric analyzers and the major function contrast of this instrument.
Several optic analytical instrument function contrasts of table 2
| Instrument | Function |
| This instrument of spectrophotometer infrared photometer light-scattering photometer fluophotometer luminescence analyzer | Ultraviolet-visible light photometric analysis of extinction near infrared, the photometric analysis of infrared photometric analysis scattering luminosity analysis of fluorescence, luminesceence analysis luminesceence analysis ultraviolet, visible light, near infrared absorbing photometric analysis scattering photometric analysis, fluorophotometric analysis, luminesceence analysis |
Claims (1)
1, a kind of integrated micro optical analyser, comprise light path part, single-chip microcomputer and auxiliary circuit part and input, single-chip microcomputer and auxiliary circuit partly are used for calculating and control, input is used for the input and output of information, it is characterized in that: described light path part (1) comprises light source constant-pressure control (4), first sets of monochromatic light sources (5) and second sets of monochromatic light sources (6), sample cell (7), optical splitter (8), photoelectric detector (9), photosensor arrays (10) and signal conditioning circuit (11); First sets of monochromatic light sources (5), sample cell (7) and photoelectric detector (9) are positioned on first light path successively, second sets of monochromatic light sources (6), sample cell (7), optical splitter (8) and photoelectric detector (9) are positioned on second light path successively, and first light path becomes 90 degree angles with second light path; Light source constant-pressure control (4) provides operating voltage according to the signal that single-chip microcomputer and auxiliary circuit partly provide for monochromatic source, and the switch of control light source; After the monochromatic homogeneous beam of sets of monochromatic light sources (5 or 6) generation passes the testing sample that is arranged in sample cell (7), a light signal part that produces shines directly on the photoelectric detector (9), measure total light intensity value and send into signal conditioning circuit (11) by it, a part shines on the photosensor arrays (10) by optical splitter (8), measure the light intensity value of each wavelength period and send into signal conditioning circuit (11), signal conditioning circuit (11) amplifies each light intensity value shaping, export to single-chip microcomputer and auxiliary circuit is partly handled, its result sends into input; Wherein,
First sets of monochromatic light sources (5) and photoelectric detector (9) assembly, or second sets of monochromatic light sources (6) and optical splitter (8), photosensor arrays (10) assembly are used for photometric analysis of extinction;
First sets of monochromatic light sources (5) and photoelectric detector (9) assembly, perhaps second sets of monochromatic light sources (6) and optical splitter (8), photosensor arrays (10) assembly are used for the scattering photometric analysis;
First sets of monochromatic light sources (5) and optical splitter (8), photosensor arrays (10) assembly are used for fluorescence analysis;
Photoelectric detector (9), perhaps optical splitter (8) and photosensor arrays (10) assembly are used for luminesceence analysis.
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Cited By (1)
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| CN101655462B (en) * | 2009-09-11 | 2011-08-10 | 中国科学院地理科学与资源研究所 | Apparatus for obtaining water quality information, method and system for recognizing water body eutrophication degree |
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