DE10120797B4 - Verfahren zur Analyse von Nukleinsäureketten - Google Patents

Verfahren zur Analyse von Nukleinsäureketten Download PDF

Info

Publication number
DE10120797B4
DE10120797B4 DE10120797A DE10120797A DE10120797B4 DE 10120797 B4 DE10120797 B4 DE 10120797B4 DE 10120797 A DE10120797 A DE 10120797A DE 10120797 A DE10120797 A DE 10120797A DE 10120797 B4 DE10120797 B4 DE 10120797B4
Authority
DE
Germany
Prior art keywords
nts
nskfs
polymerase
reaction
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE10120797A
Other languages
German (de)
English (en)
Other versions
DE10120797A1 (de
Inventor
Dmitri Tcherkassov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genovoxx GmbH
Original Assignee
Genovoxx GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genovoxx GmbH filed Critical Genovoxx GmbH
Priority to DE10120797A priority Critical patent/DE10120797B4/de
Priority to PCT/EP2002/004659 priority patent/WO2002088382A2/de
Priority to EP02732680A priority patent/EP1381698A2/de
Priority to JP2002585662A priority patent/JP2004529650A/ja
Priority to AU2002304705A priority patent/AU2002304705A1/en
Publication of DE10120797A1 publication Critical patent/DE10120797A1/de
Application granted granted Critical
Publication of DE10120797B4 publication Critical patent/DE10120797B4/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
DE10120797A 2001-04-27 2001-04-27 Verfahren zur Analyse von Nukleinsäureketten Expired - Lifetime DE10120797B4 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE10120797A DE10120797B4 (de) 2001-04-27 2001-04-27 Verfahren zur Analyse von Nukleinsäureketten
PCT/EP2002/004659 WO2002088382A2 (de) 2001-04-27 2002-04-26 Verfahren zur analyse von nukleinsäureketten
EP02732680A EP1381698A2 (de) 2001-04-27 2002-04-26 Verfahren zur analyse von nukleinsäureketten
JP2002585662A JP2004529650A (ja) 2001-04-27 2002-04-26 核酸鎖の解析方法
AU2002304705A AU2002304705A1 (en) 2001-04-27 2002-04-26 Method for analysing nucleic acid chains

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10120797A DE10120797B4 (de) 2001-04-27 2001-04-27 Verfahren zur Analyse von Nukleinsäureketten

Publications (2)

Publication Number Publication Date
DE10120797A1 DE10120797A1 (de) 2002-11-21
DE10120797B4 true DE10120797B4 (de) 2005-12-22

Family

ID=7683014

Family Applications (1)

Application Number Title Priority Date Filing Date
DE10120797A Expired - Lifetime DE10120797B4 (de) 2001-04-27 2001-04-27 Verfahren zur Analyse von Nukleinsäureketten

Country Status (5)

Country Link
EP (1) EP1381698A2 (es)
JP (1) JP2004529650A (es)
AU (1) AU2002304705A1 (es)
DE (1) DE10120797B4 (es)
WO (1) WO2002088382A2 (es)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
WO2003020968A2 (de) * 2001-08-29 2003-03-13 Genovoxx Gmbh Verfahren zur analyse von nukleinsäurekettensequenzen und der genexpression
WO2003031947A2 (de) * 2001-10-04 2003-04-17 Genovoxx Gmbh Gerät zur sequenzierung von nukleinsäuremolekülen
DE10149786B4 (de) * 2001-10-09 2013-04-25 Dmitry Cherkasov Oberfläche für Untersuchungen aus Populationen von Einzelmolekülen
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
DE10214395A1 (de) * 2002-03-30 2003-10-23 Dmitri Tcherkassov Verfahren zur Analyse von Einzelnukleotidpolymorphismen
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
SI3147292T1 (sl) 2002-08-23 2019-01-31 Illumina Cambridge Limited Označeni nukleotidi
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP1725572B1 (de) 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
JP5220596B2 (ja) * 2006-05-24 2013-06-26 独立行政法人産業技術総合研究所 ＤＮＡポリメラーゼβを用いた核酸合成法及び１分子シーケンス法
WO2007147076A2 (en) 2006-06-14 2007-12-21 Living Microsystems, Inc. Methods for the diagnosis of fetal abnormalities
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
WO2009054922A1 (en) 2007-10-19 2009-04-30 The Trustees Of Columbia University In The City Of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A2 (en) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
JP4621926B2 (ja) 2008-07-24 2011-02-02 国立大学法人九州大学 酵素基質修飾ヌクレオシド三リン酸、核酸プローブ、マルチラベル化核酸プローブ、マルチラベル化核酸プローブの製造方法および標的核酸の検出方法
JP5146785B2 (ja) * 2008-07-24 2013-02-20 国立大学法人九州大学 酵素基質修飾ヌクレオシド三リン酸誘導体
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
WO2011032040A1 (en) 2009-09-10 2011-03-17 Centrillion Technology Holding Corporation Methods of targeted sequencing
US9315860B2 (en) 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
DK2496720T3 (da) 2009-11-06 2020-09-28 Univ Leland Stanford Junior Ikke-invasiv diagnose af transplantatafstødning i organ-transplanterede patienter
JP5704481B2 (ja) * 2010-01-22 2015-04-22 国立大学法人九州大学 核酸検出用キット
KR102042253B1 (ko) 2010-05-25 2019-11-07 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Ｂａｍｂａｍ：고처리율 서열분석 데이터의 병렬 비교 분석
US9646134B2 (en) 2010-05-25 2017-05-09 The Regents Of The University Of California Bambam: parallel comparative analysis of high-throughput sequencing data
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
DE102012008375A1 (de) 2011-04-27 2012-10-31 Genovoxx Gmbh Methoden und Komponenten zur Detektion von Nukleinsäureketten
US20150086981A1 (en) 2011-05-04 2015-03-26 Genovoxx Gmbh Nucleoside-triphosphate conjugate and methods for the use thereof
EP2776962A4 (en) 2011-11-07 2015-12-02 Ingenuity Systems Inc METHODS AND SYSTEMS FOR IDENTIFICATION OF CAUSAL GENOMIC VARIANTS
WO2014026032A2 (en) 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
CN108624668B (zh) 2013-02-01 2022-12-02 加利福尼亚大学董事会 用于基因组组装及单体型定相的方法
CN108864232B (zh) 2013-03-15 2022-01-04 伊鲁米纳剑桥有限公司 修饰的核苷或核苷酸
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
CN114107458A (zh) 2013-03-15 2022-03-01 血统生物科学公司 测序免疫组库的方法
CN103484106B (zh) * 2013-09-05 2015-08-19 上海交通大学 四色荧光标记可逆终端及其在dna测序中的用途
CN105829589B (zh) 2013-11-07 2021-02-02 小利兰·斯坦福大学理事会 用于分析人体微生物组及其组分的无细胞核酸
CA2933387C (en) 2013-12-11 2023-05-02 The Regents Of The University Of California Methods for labeling dna fragments to reconstruct physical linkage and phase
AU2015296029B2 (en) 2014-08-01 2022-01-27 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
SG11201706730XA (en) 2015-02-17 2017-09-28 Dovetail Genomics Llc Nucleic acid sequence assembly
GB2554572B (en) 2015-03-26 2021-06-23 Dovetail Genomics Llc Physical linkage preservation in DNA storage
AU2016341198B2 (en) 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
KR20180116377A (ko) 2016-02-23 2018-10-24 더브테일 제노믹스 엘엘씨 게놈 어셈블리를 위한 페이징된 판독 세트의 생성 및 반수체형 페이징
IL262946B2 (en) 2016-05-13 2023-03-01 Dovetail Genomics Llc Retrieving long-range grip information from preserved samples
AU2018256358A1 (en) 2017-04-18 2019-11-07 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
CA3068273A1 (en) 2017-06-21 2018-12-27 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
CA3090102A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050642A1 (en) * 1999-02-23 2000-08-31 Caliper Technologies Corp. Sequencing by incorporation
WO2000070073A1 (en) * 1999-05-19 2000-11-23 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001025247A1 (en) * 1999-10-05 2001-04-12 Quiatech Ab Compounds for protecting hydroxyls and methods for their use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
CA2325399A1 (en) * 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
DE19844931C1 (de) * 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
JP2002537858A (ja) * 1999-03-10 2002-11-12 エーエスエム サイエンティフィック， インコーポレイテッド 核酸の配列を直接決定するための方法
GB0013276D0 (en) * 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050642A1 (en) * 1999-02-23 2000-08-31 Caliper Technologies Corp. Sequencing by incorporation
WO2000070073A1 (en) * 1999-05-19 2000-11-23 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001025247A1 (en) * 1999-10-05 2001-04-12 Quiatech Ab Compounds for protecting hydroxyls and methods for their use

Also Published As

Publication number Publication date
EP1381698A2 (de) 2004-01-21
JP2004529650A (ja) 2004-09-30
WO2002088382A3 (de) 2003-08-28
DE10120797A1 (de) 2002-11-21
WO2002088382A2 (de) 2002-11-07
AU2002304705A1 (en) 2002-11-11

Similar Documents

Publication Publication Date Title
DE10120797B4 (de) Verfahren zur Analyse von Nukleinsäureketten
DE10239504A1 (de) Verfahren zur Analyse von Nukleinsäurekettensequenzen und der Genexpression
DE10120798B4 (de) Verfahren zur Bestimmung der Genexpression
DE112007002932B4 (de) Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
DE69837913T2 (de) Verfahren zur vervielfältigung von nukleinsäure
DE69928683T2 (de) Verfahren zur amplifikation und zur sequenzierung von nukleinsäuren
DE69433487T2 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
DE69927343T2 (de) Verfahren und mittel zur analyse der nukleotidsequenz von nukleinsäuren
DE10246005A1 (de) Gerät zur Sequenzierung von Nukleinsäuremolekülen
DE69916877T2 (de) Ligations-vermittelte assemblierung und nachweis von polynukleotiden auf einem festträger
DE60208278T2 (de) System und Verfahren zum Testen von Nukleinsäuremolekülen
DE60125243T2 (de) Polynukleotid-sequenzassay
DE69720763T2 (de) Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse
DE69636895T2 (de) Die Verwendung der 3'-wesentlichen Bearbeitungswirksamkeit von DNS-Polymerase
EP1204765B1 (de) Verfahren zur relativen quantifizierung der methylierung von cytosin basen in dna-proben
DE69924140T2 (de) Bestimmung der länge von repetitiven nukleinsäure-sequenzen durch eine diskontinuierliche primerverlängerung
DE102004025744A1 (de) Oberfläche für die Analysen an einzelnen Nukleinsäuremolekülen
DE10149786B4 (de) Oberfläche für Untersuchungen aus Populationen von Einzelmolekülen
DE10214395A1 (de) Verfahren zur Analyse von Einzelnukleotidpolymorphismen
DE102004025745A1 (de) Oberfläche für die Analysen an einzelnen Molekülen
DE102004025694A1 (de) Verfahren und Oberfläche zu hochparallelen Analysen von Nukleinsäureketten
DE102004025695A1 (de) Verfahren und Oberfläche zur parallelen Sequenzierung von Nukleinsäureketten
EP2074133A2 (de) Komponenten und verfahren zur enzymatischen synthese von nukleinsäuren
DE102004025696A1 (de) Verfahren, Oberfläche und Substrate zu hochparallelen Analysen von Nukleinsäureketten
DE102004025746A1 (de) Verfahren, Oberfläche und Substrate zur hochparallelen Sequenzierung von Nukleinsäureketten

Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
8364 No opposition during term of opposition
R071 Expiry of right