DE19818481A1 - Micro-titration plate with glass base and plastic body - Google Patents

Micro-titration plate with glass base and plastic body

Info

Publication number
DE19818481A1
DE19818481A1 DE19818481A DE19818481A DE19818481A1 DE 19818481 A1 DE19818481 A1 DE 19818481A1 DE 19818481 A DE19818481 A DE 19818481A DE 19818481 A DE19818481 A DE 19818481A DE 19818481 A1 DE19818481 A1 DE 19818481A1
Authority
DE
Germany
Prior art keywords
microtiter plate
plastic body
glass base
micro
glass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19818481A
Other languages
German (de)
Inventor
Henning Vollert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis Deutschland GmbH
Aventis Pharmaceuticals Inc
Original Assignee
Hoechst Marion Roussel Deutschland GmbH
Hoechst Marion Roussel Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Marion Roussel Deutschland GmbH, Hoechst Marion Roussel Inc filed Critical Hoechst Marion Roussel Deutschland GmbH
Priority to DE19818481A priority Critical patent/DE19818481A1/en
Publication of DE19818481A1 publication Critical patent/DE19818481A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Abstract

This miniaturised microtitration plate measures 12.7 \* 8.5 cm. The novel design includes a plastic body and glass base containing 1536 round wells (3). Well diameter is 1.2-1.5 mm and the glass thickness is 0.07-0.2 mm. The cover (4) guards against evaporation.

Description

Beschreibungdescription

Die Erfindung betrifft eine miniaturisierte Mikrotiterplatte für das HTS-Screening (High Throughput Screening).The invention relates to a miniaturized microtiter plate for HTS screening (High Throughput Screening).

Bei diesem Screening ist es wünschenswert, den Verbrauch von Assaykomponenten sowie von den Substanzen aus den Screening-Libraries möglichst niedrig zu halten und den Durchsatz von Screeningassays möglichst hoch. Dies kann durch eine Miniaturisierung von Screeningassays erreicht werden. Hierbei ist es jedoch notwendig, entsprechende Mikrotiterplätten zu befüllen, die ein Assayvolumen von etwa 1 bis 2 µl aufweisen. Verfügbar sind bisher erst wenige Prototypen, die nur von bestimmten Analysengeräten verarbeitet werden können. Benötigt werden jedoch Mikrotiterplatten, die eine Analyse mit sehr empfindlichen Detektoren (mit konvokaler Optik) ermöglichen und die die Befüllung mit "Nanodispensern" erlauben. Ferner wünschenswert ist zudem ein Verdunstungsschutz.In this screening, it is desirable to reduce the consumption of Assay components as well as substances from the screening libraries as low as possible and the throughput of screening assays as possible high. This can be achieved by miniaturizing screening assays. In this case, however, it is necessary to fill appropriate microtiter plates, the one Have assay volumes of approximately 1 to 2 µl. Only a few are available so far Prototypes that can only be processed by certain analysis devices. However, microtiter plates are required which can be analyzed with very sensitive Detectors (with convocal optics) enable and fill with Allow "nanodispensers". A is also desirable Evaporation protection.

Bekannt sind Mikrotiterplatten der Fa. Greiner, 64943 Hirschberg, (Micro-Assay- Plate, 1536 wells). Hier ist das Arbeitsvolumen der Probenträger relativ hoch (4-8 µl) und sie erlauben keine "Single Molecule Detection". Das Arbeitsvolumen der Mikrotiterplatten von Corning Costar (Corning Costar Deutschland, 55924 Bodenheim) liegt zwar zwischen 1 bis 2 µl, allerdings ist der Rahmen der Mikrotiterplatten zu dünn, so daß übliche Robotersysteme die Mikrotiterplatten nicht transportieren können. Auch hier ist eine "Single Molecular Detection" nicht möglich.Microtiter plates from Greiner, 64943 Hirschberg, are known (microassay Plate, 1536 wells). Here the working volume of the sample holder is relatively high (4-8 µl) and they do not allow "single molecule detection". The working volume of the Microtiter plates from Corning Costar (Corning Costar Germany, 55924 Bodenheim) is between 1 and 2 µl, but the range is Microtiter plates too thin, so that conventional robot systems do not use the microtiter plates can transport. "Single molecular detection" is not possible here either.

Hier will die Erfindung Abhilfe schaffen. The invention seeks to remedy this.  

Erfindungsgemäß geschieht dies durch eine miniaturisierte Mikrotiterplatte mit einer Größe von 12,7×8,5 cm, die dadurch gekennzeichnet ist, daß die Mikrotiterplatte (Körper aus Kunststoff, Boden aus Glas), 1536 runde Gefäße (Wells) hat, der Durchmesser der Gefäße (Wells) ungefähr 1,2 bis 1,5 mm ist, der Boden der Mikrotiterplatte aus Glas besteht und eine Schichtdicke von 0,07-0,2 mm, bevorzugt 0,14 mm, aufweist und die Mikrotiterplatte einen Deckel als Verdunstungsschutz hat.According to the invention, this is done with a miniaturized microtiter plate Size of 12.7 × 8.5 cm, which is characterized in that the microtiter plate (Body made of plastic, bottom made of glass), 1536 round vessels (Wells), the Diameter of the vessels (wells) is approximately 1.2 to 1.5 mm, the bottom of the Microtiter plate made of glass and a layer thickness of 0.07-0.2 mm, preferably 0.14 mm, and the microtiter plate has a lid as Has evaporation protection.

Bei der Herstellung von Mikrotiterplatten ist es wichtig, das richtige Material zu verwenden. Der Körper der Mikrotiterplatte besteht aus Kunststoff, wie z. B. Polystyrol, Polypropylen, Polycarbonat, Vectra, Hostalen. Die Mikrotiterplatten werden in der Regel im Spritzgußverfahren hergestellt. Nach dem Spritzen kühlt der Kunststoff ab. Dabei kann sich die Mikrotiterplatte verbiegen (da die Abkühlung lokal unterschiedlich schnell erfolgt). Somit ist es günstig ein Material zu verwenden, das nur eine sehr geringe "Verkrümmung" erzeugt.When manufacturing microtiter plates, it is important to choose the right material use. The body of the microtiter plate is made of plastic, such as. B. Polystyrene, polypropylene, polycarbonate, Vectra, Hostalen. The microtiter plates are usually produced by injection molding. After spraying, the cools Plastic. The microtiter plate can bend (because the cooling is local takes place at different speeds). It is therefore convenient to use a material that produces only a very slight "curvature".

Der Deckel der Mikrotiterplatte ist ebenfalls aus Kunststoff und sitzt auf der Mikrotiterplatte formschlüssig auf. Die Schichtdicke des Bodens (Material: Glas) der Mikrotiterplatte (0,07-0,20 mm) als auch der Durchmesser der Gefäße (ca. 1,2-1,5 mm) erlauben die Analyse der Mikrotiterplatte mit Hilfe einer konvokalen Optik. Die Verwendung einer konvokalen Optik hat folgende Vorteile:
The lid of the microtiter plate is also made of plastic and sits positively on the microtiter plate. The layer thickness of the base (material: glass) of the microtiter plate (0.07-0.20 mm) as well as the diameter of the tubes (approx. 1.2-1.5 mm) allow the microtiter plate to be analyzed with the help of convocal optics. Using a convocal lens has the following advantages:

  • 1. Die Sensitivität ist sehr hoch (im Vergleich zu nicht konvokalen Optiken), da u. U. sogar einzelne Moleküle detektiert werden können (Single Molecule Detection)1. The sensitivity is very high (compared to non-convocal optics) because u. Under certain circumstances, even single molecules can be detected (single molecule detection)
  • 2. Aufgrund der hohen Sensitivität kann die Meßzeit niedriger sein und somit die gesamte Analysegeschwindigkeit einer Mikrotiterplatte erhöht werden (im Vergleich zu vielen nicht konvokalen Optiken).2. Due to the high sensitivity, the measurement time can be shorter and thus the entire analysis speed of a microtiter plate can be increased (in comparison too many non-convocal optics).
  • 3. Da der Focus einer konvokalen Optik sehr klein ist (meist deutlich unter 10 µm), ist die Detektion von Hintergrundsignalen stark reduziert und somit das Signal/Rauschverhältnis besser (im Vergleich zu nicht konvokalen Optiken).3. Since the focus of a convocal optic is very small (usually well below 10 µm), the detection of background signals is greatly reduced and thus that Signal / noise ratio better (compared to non-convocal optics).

Die Anzahl der Gefäße (Wells) der miniaturisierten Mikrotiterplatte kann auch variieren.The number of tubes (wells) of the miniaturized microtiter plate can also be vary.

Im folgenden werden mögliche Ausgestaltungen der erfindungsgemäßen Mikrotiterplatte anhand der Fig. 1 bis 3 näher beschrieben.Possible configurations of the microtiter plate according to the invention are described in more detail below with reference to FIGS. 1 to 3.

Fig. 1 Perspektivische Darstellung der Mikrotiterplatte mit abgehobenem Deckel Fig. 1 perspective view of the microtiter plate with the cover removed

Fig. 2 Schnitt gemäß Ebene II-II aus Fig. 1 Fig. 2 shows a section according to plane II-II of FIG. 1

Fig. 3 Schnitt gemäß Ebene III-III aus Fig. 1. Fig. 3 section according to plane III-III in FIG. 1.

In Fig. 1 ist die Mikrotiterplatte mit den Gefäßen (3) perspektivisch dargestellt. Der Rahmen (1) hat eine Länge von a = 127 mm und eine Breite von b = 85 mm. Der Deckel (4) mit den Vorsprüngen (5) ist in abgehobenem Zustand gezeigt.In Fig. 1, the microtiter plate with the vessels ( 3 ) is shown in perspective. The frame ( 1 ) has a length of a = 127 mm and a width of b = 85 mm. The cover ( 4 ) with the projections ( 5 ) is shown in the raised state.

In Fig. 2 ist ein Schnitt gemäß Ebene II-II dargestellt. Der Glasboden (2) ist unter der Mikrotiterplatte befestigt. Der Randabstand (a1) beträgt 3-8 mm, bevorzugt 6 mm, und der Abstand (a2) 6-11 mm, bevorzugt 9,5 mm. Der entsprechende Randabstand (b1) in Fig. 3 beträgt ebenfalls 3-8 mm, bevorzugt 6 mm, und der Abstand (b2) 4-9 mm, bevorzugt 6,5 mm. Die Rahmenhöhe der Mikrotiterplatte beträgt c = 6-15 mm, bevorzugt 6 mm, und die Innenhöhe c1 3-12 mm, bevorzugt 3 mm. Der Gefäßdurchmesser (d) liegt zwischen 1,0 und 1,5 mm, bevorzugt bei 1,3 mm, der Gefäßabstand (a3) bei 2,25 mm und die Gefäßhöhe (h) zwischen 2,0 und 7,0 mm.In FIG. 2 shows a section according to plane II-II. The glass bottom ( 2 ) is attached under the microtiter plate. The edge distance (a1) is 3-8 mm, preferably 6 mm, and the distance (a2) 6-11 mm, preferably 9.5 mm. The corresponding edge distance (b1) in FIG. 3 is likewise 3-8 mm, preferably 6 mm, and the distance (b2) 4-9 mm, preferably 6.5 mm. The frame height of the microtiter plate is c = 6-15 mm, preferably 6 mm, and the inner height c1 3-12 mm, preferably 3 mm. The vessel diameter (d) is between 1.0 and 1.5 mm, preferably 1.3 mm, the vessel distance (a3) is 2.25 mm and the vessel height (h) is between 2.0 and 7.0 mm.

Claims (1)

Miniaturisierte Mikrotiterplatte mit einer Größe von 12,7×8,5 cm, dadurch gekennzeichnet, daß die Mikrotiterplatte (Körper aus Kunststoff, Boden aus Glas), 1536 runde Gefäße (Wells) (3) hat, der Durchmesser der Gefäße (Wells) (d) 1,2 bis 1,5 mm ist, der Boden der Mikrotiterplatte (2) aus Glas besteht und eine Schichtdicke von 0,07-0,2 mm aufweist und die Mikrotiterplatte einen Deckel (4) als Verdunstungsschutz hat.Miniaturized microtiter plate with a size of 12.7 × 8.5 cm, characterized in that the microtiter plate (body made of plastic, bottom made of glass) has 1536 round vessels (wells) ( 3 ), the diameter of the vessels (wells) ( d) 1.2 to 1.5 mm, the bottom of the microtiter plate ( 2 ) consists of glass and has a layer thickness of 0.07-0.2 mm and the microtiter plate has a lid ( 4 ) as evaporation protection.
DE19818481A 1998-03-27 1998-04-24 Micro-titration plate with glass base and plastic body Withdrawn DE19818481A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19818481A DE19818481A1 (en) 1998-03-27 1998-04-24 Micro-titration plate with glass base and plastic body

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE29805613U DE29805613U1 (en) 1998-03-27 1998-03-27 Miniaturized microtiter plate for HTS screening
DE19818481A DE19818481A1 (en) 1998-03-27 1998-04-24 Micro-titration plate with glass base and plastic body

Publications (1)

Publication Number Publication Date
DE19818481A1 true DE19818481A1 (en) 1999-10-14

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DE19818481A Withdrawn DE19818481A1 (en) 1998-03-27 1998-04-24 Micro-titration plate with glass base and plastic body

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE29805613U Expired - Lifetime DE29805613U1 (en) 1998-03-27 1998-03-27 Miniaturized microtiter plate for HTS screening

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE20100345U1 (en) * 2001-01-09 2002-05-23 Evotec Biosystems Ag sample carrier
DE10117723A1 (en) * 2001-04-09 2002-10-17 Evotec Ag Carrier for biological or synthetic samples has a sample holding plate with reservoirs and a dosing plate with projections, fitted with membranes, of an optically transparent material with trouble-free light beam transparency
DE102004041941A1 (en) * 2004-08-30 2006-03-02 P.A.L.M. Microlaser Technologies Ag Recording device for biological objects and method and apparatus for processing in the recording unit recorded biological objects

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19852947A1 (en) * 1998-11-12 2000-05-18 Univ Schiller Jena Micro-liter automatic dispensing apparatus comprises needle moving vertically to penetrate cover over micro-titration plate, to dispense liquid into cells
DE19943443A1 (en) * 1999-09-11 2001-04-12 Inst Mikrotechnik Mainz Gmbh Device and method for the planarization of titer plates in screening and synthesis systems
US20020132360A1 (en) 2000-11-17 2002-09-19 Flir Systems Boston, Inc. Apparatus and methods for infrared calorimetric measurements
US6821787B2 (en) 2000-11-17 2004-11-23 Thermogenic Imaging, Inc. Apparatus and methods for infrared calorimetric measurements

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2633293A1 (en) * 1976-07-23 1978-01-26 Bayer Ag Dispersions of polyaddition prods. of polyisocyanates in hydroxy cpds. - useful as polyol components for prodn. of polyurethanes, by reaction with polyisocyanates
US4725388A (en) * 1982-10-12 1988-02-16 Dynatech Laboratories, Inc. Non-fluorescent vessels for holding test samples in fluorescent assays
EP0449434A2 (en) * 1990-03-30 1991-10-02 Beckman Instruments, Inc. Multi-cell module for spectrophotometry
US5487872A (en) * 1994-04-15 1996-01-30 Molecular Device Corporation Ultraviolet radiation transparent multi-assay plates
DE19628178C1 (en) * 1996-07-12 1997-09-18 Bruker Franzen Analytik Gmbh Loading matrix-assisted laser desorption-ionisation sample plate for mass spectrometric analysis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824230A (en) * 1985-12-13 1989-04-25 E. I. Du Pont De Nemours And Company Visualization device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2633293A1 (en) * 1976-07-23 1978-01-26 Bayer Ag Dispersions of polyaddition prods. of polyisocyanates in hydroxy cpds. - useful as polyol components for prodn. of polyurethanes, by reaction with polyisocyanates
US4725388A (en) * 1982-10-12 1988-02-16 Dynatech Laboratories, Inc. Non-fluorescent vessels for holding test samples in fluorescent assays
EP0449434A2 (en) * 1990-03-30 1991-10-02 Beckman Instruments, Inc. Multi-cell module for spectrophotometry
US5487872A (en) * 1994-04-15 1996-01-30 Molecular Device Corporation Ultraviolet radiation transparent multi-assay plates
DE19628178C1 (en) * 1996-07-12 1997-09-18 Bruker Franzen Analytik Gmbh Loading matrix-assisted laser desorption-ionisation sample plate for mass spectrometric analysis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Analytical Chemistry 69 (1997) 543-551 *
Prospekt Firma Hellma (1994): Mikrotestpallten aus Quarzglas *
Prospekt Firma Radleys (1997): Specialist Micro Titer Plates & Accessoires *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE20100345U1 (en) * 2001-01-09 2002-05-23 Evotec Biosystems Ag sample carrier
US7393506B2 (en) 2001-01-09 2008-07-01 Evotec Oai Ag Sample carrier
DE10117723A1 (en) * 2001-04-09 2002-10-17 Evotec Ag Carrier for biological or synthetic samples has a sample holding plate with reservoirs and a dosing plate with projections, fitted with membranes, of an optically transparent material with trouble-free light beam transparency
DE102004041941A1 (en) * 2004-08-30 2006-03-02 P.A.L.M. Microlaser Technologies Ag Recording device for biological objects and method and apparatus for processing in the recording unit recorded biological objects
DE102004041941B4 (en) * 2004-08-30 2007-01-11 P.A.L.M. Microlaser Technologies Ag Method for obtaining biological objects with a recording unit

Also Published As

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