DE3789905D1 - Verfahren zum Nachweis von elektrophoretisch getrennten Oligonukleotiden. - Google Patents

Verfahren zum Nachweis von elektrophoretisch getrennten Oligonukleotiden.

Info

Publication number
DE3789905D1
DE3789905D1 DE3789905T DE3789905T DE3789905D1 DE 3789905 D1 DE3789905 D1 DE 3789905D1 DE 3789905 T DE3789905 T DE 3789905T DE 3789905 T DE3789905 T DE 3789905T DE 3789905 D1 DE3789905 D1 DE 3789905D1
Authority
DE
Germany
Prior art keywords
detection
electrophoretically separated
separated oligonucleotides
oligonucleotides
electrophoretically
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3789905T
Other languages
English (en)
Other versions
DE3789905T2 (de
Inventor
Steven Fung
Sam Lee Woo
Steven M Menchen
Charles R Connell
Cheryl Heiner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25248986&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3789905(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Applied Biosystems Inc filed Critical Applied Biosystems Inc
Publication of DE3789905D1 publication Critical patent/DE3789905D1/de
Application granted granted Critical
Publication of DE3789905T2 publication Critical patent/DE3789905T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
DE3789905T 1986-02-07 1987-02-04 Verfahren zum Nachweis von elektrophoretisch getrennten Oligonukleotiden. Expired - Lifetime DE3789905T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/827,348 US4855225A (en) 1986-02-07 1986-02-07 Method of detecting electrophoretically separated oligonucleotides

Publications (2)

Publication Number Publication Date
DE3789905D1 true DE3789905D1 (de) 1994-07-07
DE3789905T2 DE3789905T2 (de) 1995-01-19

Family

ID=25248986

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3789905T Expired - Lifetime DE3789905T2 (de) 1986-02-07 1987-02-04 Verfahren zum Nachweis von elektrophoretisch getrennten Oligonukleotiden.

Country Status (4)

Country Link
US (1) US4855225A (de)
EP (1) EP0233053B1 (de)
JP (1) JP2678597B2 (de)
DE (1) DE3789905T2 (de)

Families Citing this family (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5171534A (en) * 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
USRE43096E1 (en) 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
US6207421B1 (en) 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5863403A (en) * 1984-03-29 1999-01-26 The Board Of Regents Of The University Of Nebraska Digital DNA typing
US6004446A (en) * 1984-03-29 1999-12-21 Li-Cor, Inc. DNA Sequencing
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
JP2527340B2 (ja) * 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
EP0310312A3 (de) * 1987-10-02 1990-08-16 M.L. Technology Ventures, L.P. Nichtnukleotide Reagenzien für Endsubstituierung von Oligonukleotiden
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
PT88665B (pt) * 1987-10-05 1992-12-31 Ml Tecnology Ventures Lp Metodo para a marcacao com ester de acridinio e purificacao de sondas nucleotidicas
FI80476C (fi) * 1987-10-09 1990-06-11 Orion Yhtymae Oy Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning.
EP0317239A3 (de) * 1987-11-13 1990-01-17 Native Plants Incorporated Verfahren und Vorrichtung zum Nachweis von Restriktionsfragmentlängenpolymorphien
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
JP2804038B2 (ja) * 1988-02-24 1998-09-24 株式会社日立製作所 塩基配列決定方法
DE3807975C2 (de) * 1988-03-10 2002-03-07 Karl Otto Greulich Verfahren zur optischen Charakterisierung von Nukleinsäuren und Oligonukleotiden
US4962045A (en) * 1988-05-02 1990-10-09 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
US5003059A (en) * 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5925525A (en) * 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6309822B1 (en) * 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US6406844B1 (en) 1989-06-07 2002-06-18 Affymetrix, Inc. Very large scale immobilized polymer synthesis
US6919211B1 (en) 1989-06-07 2005-07-19 Affymetrix, Inc. Polypeptide arrays
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US6379895B1 (en) 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6955915B2 (en) 1989-06-07 2005-10-18 Affymetrix, Inc. Apparatus comprising polymers
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5366860A (en) * 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
AU638568B2 (en) * 1990-02-26 1993-07-01 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A method for the fluorescent detection of a dna sequence in real time
US6506558B1 (en) 1990-03-07 2003-01-14 Affymetrix Inc. Very large scale immobilized polymer synthesis
US5124366A (en) * 1990-11-05 1992-06-23 The Celotex Corporation Polyisocyanurate foams made with polyester polyols and chlorodifluoromethane as a blowing agent
DE69132843T2 (de) 1990-12-06 2002-09-12 Affymetrix Inc N D Ges D Staat Identifizierung von Nukleinsäuren in Proben
US5210412A (en) * 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
WO1992013629A1 (en) * 1991-01-31 1992-08-20 Wayne State University A method for analyzing an organic sample
WO1992020821A1 (en) * 1991-05-20 1992-11-26 Pharmaceutical Discovery Corporation cDNA-PHOTOFLUOR PROBE AND METHODS OF MAKING, ASSAYING AND USING SAME
US5451343A (en) * 1991-05-20 1995-09-19 Spectra Group Limited, Inc. Fluorone and pyronin y derivatives
DE69217497T2 (de) * 1991-09-18 1997-06-12 Affymax Tech Nv Verfahren zur synthese der verschiedenen sammlungen von oligomeren
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5677195A (en) * 1991-11-22 1997-10-14 Affymax Technologies N.V. Combinatorial strategies for polymer synthesis
US6943034B1 (en) 1991-11-22 2005-09-13 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
US6849462B1 (en) 1991-11-22 2005-02-01 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
US6468740B1 (en) 1992-11-05 2002-10-22 Affymetrix, Inc. Cyclic and substituted immobilized molecular synthesis
WO1993014224A1 (en) * 1992-01-14 1993-07-22 Applied Biosystems, Inc. Size-calibration dna fragment mixture and method
WO1993018177A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia Diagnosis of cystic fibrosis using allele specific multiplex polymerase chain reactions
EP0633944B1 (de) * 1992-03-31 2000-11-08 Abbott Laboratories Verfahren zur mehrfachen ligase-kettenreaktion
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US5869252A (en) * 1992-03-31 1999-02-09 Abbott Laboratories Method of multiplex ligase chain reaction
EP0636186B1 (de) 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Proben zusammensetzung und verfahren
US5302731A (en) * 1992-07-13 1994-04-12 Becton, Dickinson And Company Fluorescent pH indicators
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US5395752A (en) * 1993-03-19 1995-03-07 Ciba Corning Diagnostics Corp. Long emission wavelength chemiluminescent compounds and their use in test assays
US5503805A (en) * 1993-11-02 1996-04-02 Affymax Technologies N.V. Apparatus and method for parallel coupling reactions
US6165778A (en) * 1993-11-02 2000-12-26 Affymax Technologies N.V. Reaction vessel agitation apparatus
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5869255A (en) * 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
US6028190A (en) * 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5599666A (en) * 1994-03-28 1997-02-04 Promega Corporation Allelic ladders for short tandem repeat loci
US7323298B1 (en) 1994-06-17 2008-01-29 The Board Of Trustees Of The Leland Stanford Junior University Microarray for determining the relative abundances of polynuceotide sequences
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US6103465A (en) * 1995-02-14 2000-08-15 The Perkin-Elmer Corporation Methods and reagents for typing HLA class I genes
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US6127134A (en) * 1995-04-20 2000-10-03 Carnegie Mellon University Difference gel electrophoresis using matched multiple dyes
US6426190B1 (en) * 1995-04-20 2002-07-30 Carnegie Mellon University Difference detection methods using matched multiple dyes
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
CA2225531A1 (en) * 1995-06-23 1997-01-09 Baylor College Of Medicine Alternative dye-labeled primers, ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated dna analysis and homogeneous amplification/detection assays
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5861287A (en) * 1995-06-23 1999-01-19 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5728529A (en) * 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
EP0880598A4 (de) 1996-01-23 2005-02-23 Affymetrix Inc Verfahren zur analyse von nukleinsäure
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US7244622B2 (en) 1996-04-03 2007-07-17 Applera Corporation Device and method for multiple analyte detection
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7825237B2 (en) * 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US6156512A (en) * 1996-05-23 2000-12-05 Promega Corp Allelic ladders for short tandem repeat loci
US20040266706A1 (en) * 2002-11-05 2004-12-30 Muthiah Manoharan Cross-linked oligomeric compounds and their use in gene modulation
US7550570B2 (en) * 2000-05-25 2009-06-23 Applied Biosystems, Llc. 4,7-dichlororhodamine dyes labeled polynucleotides
US6017712A (en) * 1996-06-27 2000-01-25 Lee; Linda 4,7-dichlororhodamine dyes
US6080852A (en) 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
AU4565697A (en) * 1996-10-07 1998-05-05 Amersham International Plc Analysis of carbohydrates
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
CA2270132A1 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
CA2268740C (en) 1996-11-06 2010-07-20 Sequenom, Inc. High density immobilization of nucleic acids
US6471866B1 (en) 1996-11-13 2002-10-29 Transgenomic, Inc. Process for performing polynucleotide separations
US5972222A (en) * 1996-11-13 1999-10-26 Transgenomic, Inc. Process for performing polynucleotide separations
US6030527A (en) * 1996-11-13 2000-02-29 Transgenomic, Inc. Apparatus for performing polynucleotide separations using liquid chromatography
US6017457A (en) * 1996-11-13 2000-01-25 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US5997742A (en) * 1996-11-13 1999-12-07 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
US6174441B1 (en) 1996-11-13 2001-01-16 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
JP2000516638A (ja) 1997-06-10 2000-12-12 トランスジエノミツク・インコーポレーテツド 液体クロマトグラフィーを使用するポリヌクレオチド分離を実施するための系および方法
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
AU752932B2 (en) 1997-10-09 2002-10-03 Transgenomic, Inc. Modifying double stranded DNA to enhance separations by matched ion polynucleotide chromatography
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6583168B1 (en) 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US6372130B1 (en) 1997-12-05 2002-04-16 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6908770B1 (en) 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6545264B1 (en) 1998-10-30 2003-04-08 Affymetrix, Inc. Systems and methods for high performance scanning
DE60016898T2 (de) * 1999-02-18 2006-03-30 The Regents Of The University Of California, Oakland Phthalamid-lanthanid komplexe zur verwendung als lumineszenzmarker
US7022517B1 (en) 1999-07-16 2006-04-04 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
US6589779B1 (en) 1999-07-16 2003-07-08 Board Of Regents, The University Of Texas System General signaling protocol for chemical receptors in immobilized matrices
DE60135092D1 (de) 2000-01-31 2008-09-11 Univ Texas Tragbare vorrichtung mit einer sensor-array-anordnung
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
JP2004516810A (ja) 2000-06-07 2004-06-10 リ−コール インコーポレーティッド 電荷スイッチヌクレオチド
US6397150B1 (en) 2000-07-27 2002-05-28 Visible Genetics Inc. Method and apparatus for sequencing of DNA using an internal calibrant
US6548251B1 (en) 2000-09-05 2003-04-15 Fidelity Systems, Inc. Inhibition of molecular and biological processes using modified oligonucleotides
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7118907B2 (en) * 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
AU2003244370A1 (en) * 2002-02-05 2003-09-02 Baylor College Of Medecine Substituted 4,4-difluoro-4-bora-3a, 4a-diaza-s-indacene compounds for 8-color dna sequencing
JP2006505832A (ja) * 2002-02-26 2006-02-16 フアルマシア・コーポレーシヨン 配列決定システム計算機
AU2003228711C1 (en) 2002-04-26 2010-01-07 Board Of Regents, The University Of Texas System Method and system for the detection of cardiac risk factors
US7582260B2 (en) * 2002-07-18 2009-09-01 Montana State University Zwitterionic dyes for labeling in proteomic and other biological analyses
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2552537A1 (en) * 2003-12-30 2006-01-05 The Regents Of The University Of California Aromatic triamide-lanthanide complexes
WO2005080605A2 (en) * 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US8105849B2 (en) 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
WO2006034036A1 (en) 2004-09-16 2006-03-30 Applera Corporation Fluorescent dye compounds, conjugates and uses thereof
JP4005077B2 (ja) * 2004-11-22 2007-11-07 Ｎｅｃエレクトロニクス株式会社 半導体装置の製造方法及び粘性液体の塗膜方法
JP2008528040A (ja) 2005-02-01 2008-07-31 アジェンコート バイオサイエンス コーポレイション ビーズベースの配列決定のための試薬、方法およびライブラリー
EP2233582A1 (de) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
US8377398B2 (en) 2005-05-31 2013-02-19 The Board Of Regents Of The University Of Texas System Methods and compositions related to determination and use of white blood cell counts
WO2007120265A2 (en) 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
US20090062129A1 (en) * 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
CA2659251C (en) 2006-07-10 2016-06-14 The Regents Of The University Of California Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
DK2423201T3 (en) 2006-08-15 2018-01-15 Univ California Luminescent macrocyclic lanthanide complexes
EP2114905B1 (de) * 2007-01-25 2015-04-15 Lumiphore, Inc. Mehrfarbige zeitgesteuerte fluorophore auf basis makrozyklischer lanthanid-komplexe
ES2797951T3 (es) 2007-04-04 2020-12-04 Ande Corp Análisis integrado de ácidos nucleicos
WO2009046149A1 (en) * 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
WO2010051544A2 (en) * 2008-10-31 2010-05-06 Lumiphore, Inc. Rapid homogeneous diagnostic testing platform based on lanthanide fluorescent resonance energy transfer
JP2012529908A (ja) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド 法医学的ｄｎａの定量化のための改善された方法
EP2470513B1 (de) 2009-08-24 2014-10-08 Lumiphore, Inc. Makrozyklische hopo-chelatoren
US20110189088A1 (en) * 2009-12-24 2011-08-04 Lumiphore, Inc. Radiopharmaceutical complexes
KR101982627B1 (ko) 2011-05-12 2019-05-27 에이엔디이 코포레이션 Ｓｔｒ 유전자좌의 신속한 다중 증폭 방법 및 조성법
US9289502B2 (en) 2013-03-08 2016-03-22 Emerald Therapeutics, Inc. Preparation of oligo conjugates
US11453652B2 (en) 2013-03-15 2022-09-27 Lumiphore, Inc. Di-macrocycles
EP3408253A1 (de) 2016-01-28 2018-12-05 Acatechol, Inc. Photospaltbare primerzusammensetzungen und verfahren zur verwendung
CN112566986A (zh) 2018-08-10 2021-03-26 生命技术公司 经硅取代的罗丹明染料和染料缀合物
WO2022124789A1 (ko) * 2020-12-09 2022-06-16 에스에프씨 주식회사 소광자 및 이의 용도

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4238195A (en) * 1979-01-18 1980-12-09 Miles Laboratories, Inc. Fluorescer-labeled specific binding assays
CA1121345A (en) * 1979-03-05 1982-04-06 Robert A. Yoshida Method for competitive protein binding assays inhibiting non-specific interference
US4318846A (en) * 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
JPS5782387A (en) * 1980-10-29 1982-05-22 Syva Co Novel ether substituted fluorescein compound
AU555213B2 (en) * 1981-02-17 1986-09-18 Abbott Laboratories N-substituted-amido-fluoresein derivatives
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
CA1222705A (en) * 1983-07-14 1987-06-09 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
US4737454A (en) * 1983-07-14 1988-04-12 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
ZA841924B (en) * 1983-07-25 1984-10-31 Becton Dickinson Co Chromogenic tracers for use in an assay
SE466208B (sv) * 1983-12-20 1992-01-13 California Inst Of Techn Enkelstraengad aminoderivat av oligonukleotider och foerfarande foer framstaellning daerav
GB2155176B (en) * 1984-01-16 1988-05-11 California Inst Of Techn Sequencing dna
JPH0690200B2 (ja) * 1984-01-16 1994-11-14 カリフオルニア・インステイテユ−ト・オブ・テクノロジ− オリゴヌクレオチドの分析法
JPS60242368A (ja) * 1984-05-16 1985-12-02 Hitachi Ltd 核酸塩基配列決定方法
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
DE3642939A1 (de) * 1986-06-03 1987-12-10 Europ Lab Molekularbiolog Verfahren zur dna-markierung
JP2527340B2 (ja) * 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体

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EP0233053A3 (en) 1989-03-22
JPS62249049A (ja) 1987-10-30
EP0233053A2 (de) 1987-08-19
JP2678597B2 (ja) 1997-11-17
US4855225A (en) 1989-08-08
DE3789905T2 (de) 1995-01-19

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