DE4411588C1 - Puffer für DNA- und RNA-Polymerase-Reaktionen - Google Patents

Puffer für DNA- und RNA-Polymerase-Reaktionen

Info

Publication number
DE4411588C1
DE4411588C1 DE4411588A DE4411588A DE4411588C1 DE 4411588 C1 DE4411588 C1 DE 4411588C1 DE 4411588 A DE4411588 A DE 4411588A DE 4411588 A DE4411588 A DE 4411588A DE 4411588 C1 DE4411588 C1 DE 4411588C1
Authority
DE
Germany
Prior art keywords
betaine
pcr
buffer according
rna
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE4411588A
Other languages
German (de)
English (en)
Inventor
Thomas Weissensteiner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutches Rheuma Forschungszentrum Berlin DRFZ
Original Assignee
Deutches Rheuma Forschungszentrum Berlin DRFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutches Rheuma Forschungszentrum Berlin DRFZ filed Critical Deutches Rheuma Forschungszentrum Berlin DRFZ
Priority to DE4411588A priority Critical patent/DE4411588C1/de
Application granted granted Critical
Publication of DE4411588C1 publication Critical patent/DE4411588C1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DE4411588A 1994-03-30 1994-03-30 Puffer für DNA- und RNA-Polymerase-Reaktionen Expired - Lifetime DE4411588C1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE4411588A DE4411588C1 (de) 1994-03-30 1994-03-30 Puffer für DNA- und RNA-Polymerase-Reaktionen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4411588A DE4411588C1 (de) 1994-03-30 1994-03-30 Puffer für DNA- und RNA-Polymerase-Reaktionen

Publications (1)

Publication Number Publication Date
DE4411588C1 true DE4411588C1 (de) 1995-09-28

Family

ID=6514580

Family Applications (1)

Application Number Title Priority Date Filing Date
DE4411588A Expired - Lifetime DE4411588C1 (de) 1994-03-30 1994-03-30 Puffer für DNA- und RNA-Polymerase-Reaktionen

Country Status (1)

Country Link
DE (1) DE4411588C1 (es)

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2733515A1 (fr) * 1995-04-27 1996-10-31 Cis Bio Int Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications
EP0742838A1 (en) * 1994-01-31 1996-11-20 The Regents Of The University Of California Methods for the elimination of dna sequencing artifacts
EP0808906A1 (en) * 1996-05-23 1997-11-26 COLPAN, Metin, Dr. Use of an osmolyte for reducing non-covalent interactions of biological molecules to inert surfaces
EP0821059A2 (en) * 1996-07-25 1998-01-28 The Institute Of Physical & Chemical Research Method for reverse transcription
EP0828853A1 (en) * 1994-10-18 1998-03-18 Genzyme Corporation Improved method for nucleotide sequence amplification
WO1998048053A1 (en) * 1997-04-21 1998-10-29 Epicentre Technologies Corporation Reverse transcription method
WO1999046400A1 (en) 1998-03-13 1999-09-16 Life Technologies, Inc. Compositions and methods for enhanced synthesis of nucleic acid molecules
EP1002126A1 (en) * 1997-06-25 2000-05-24 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
US6150094A (en) * 1996-05-23 2000-11-21 Qiagen Gmbh Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces
US6270962B1 (en) 1995-01-30 2001-08-07 The Regents Of The University Of California Methods for the elimination of DNA sequencing artifacts
DE10105208A1 (de) * 2001-02-06 2002-08-14 Ihf Inst Fuer Hormon Und Fortp Verfahren zur Herstellung von cDNA
WO2003014398A2 (en) * 2001-01-03 2003-02-20 Transgenomic, Inc. Methods and compositions for mutation detection by liquid chromatography
WO2004048596A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Methods for using primers that encode one strand of a double-stranded promoter
US6780588B2 (en) 2001-05-07 2004-08-24 Applera Corporation Methods for the reduction of stutter in microsatellite amplification
US6783940B2 (en) 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR
EP1452593A1 (en) * 2001-11-14 2004-09-01 Toyo Boseki Kabushiki Kaisha Dna synthesis promoters, dna polymerase-associated factors and utilization thereof
US6841349B2 (en) 2001-05-07 2005-01-11 Applera Corporation Applied Biosystems Group Methods for the reduction of stutter in microsatellite amplification
EP1621638A1 (en) * 2003-05-07 2006-02-01 Takara Bio Inc. Method of analyzing gene introduction site
GB2420560A (en) * 2004-11-30 2006-05-31 Bioline Ltd A method for increasing the specificity of enzymatic DNA synthesis
WO2009006438A2 (en) 2007-06-29 2009-01-08 Epicentre Technologies Corporation Copy dna and sense rna
US7915450B2 (en) 1998-11-12 2011-03-29 Life Technologies Corporation Transfection reagents
US10195280B2 (en) 2014-07-15 2019-02-05 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rees. W.A.et.al., Biochemistry 32, 1993, 137-44 *

Cited By (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0742838A1 (en) * 1994-01-31 1996-11-20 The Regents Of The University Of California Methods for the elimination of dna sequencing artifacts
EP0742838A4 (en) * 1994-01-31 2000-01-05 Univ California METHODS FOR ELIMINATING DNA SEQUEN AGE ARTIFACTS
EP0828853A4 (en) * 1994-10-18 1998-05-13 Genzyme Corp IMPROVED METHOD FOR AMPLIFICATING NUCLEOTIDE SEQUENCES
EP0828853A1 (en) * 1994-10-18 1998-03-18 Genzyme Corporation Improved method for nucleotide sequence amplification
US6270962B1 (en) 1995-01-30 2001-08-07 The Regents Of The University Of California Methods for the elimination of DNA sequencing artifacts
FR2733515A1 (fr) * 1995-04-27 1996-10-31 Cis Bio Int Tampon, procede et methode d'evaluation des conditions optimales, d'amplification de sequences cibles d'acide nucleique, et leurs applications
US6150094A (en) * 1996-05-23 2000-11-21 Qiagen Gmbh Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces
EP0808906A1 (en) * 1996-05-23 1997-11-26 COLPAN, Metin, Dr. Use of an osmolyte for reducing non-covalent interactions of biological molecules to inert surfaces
EP0821059A3 (en) * 1996-07-25 1998-04-22 The Institute Of Physical & Chemical Research Method for reverse transcription
EP1243661A1 (en) * 1996-07-25 2002-09-25 The Institute of Physical and Chemical Research Method for reverse transcription
EP0821059A2 (en) * 1996-07-25 1998-01-28 The Institute Of Physical & Chemical Research Method for reverse transcription
WO1998048053A1 (en) * 1997-04-21 1998-10-29 Epicentre Technologies Corporation Reverse transcription method
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
US6875568B2 (en) 1997-06-25 2005-04-05 Invitrogen Corporation Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
EP1002126A1 (en) * 1997-06-25 2000-05-24 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
EP1002126A4 (en) * 1997-06-25 2004-11-03 Life Technologies Inc IMPROVED METHOD FOR ISOLATING AND RECOVERING TARGET DNA OR RNA MOLECULES HAVING A DESIRED NUCLEOTIDE SEQUENCE
US6268133B1 (en) 1997-06-25 2001-07-31 Invitrogen Corporation Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
WO1999046400A1 (en) 1998-03-13 1999-09-16 Life Technologies, Inc. Compositions and methods for enhanced synthesis of nucleic acid molecules
EP1062360A4 (en) * 1998-03-13 2009-11-11 Life Technologies Corp COMPOUNDS AND METHODS FOR VERTÄARKERRN NUCLEIC ACID MOLECULAR SYNTHASE
EP1062360A1 (en) * 1998-03-13 2000-12-27 Life Technologies, Inc. Compositions and methods for enhanced synthesis of nucleic acid molecules
US7344863B2 (en) 1998-03-13 2008-03-18 Invitrogen Corporation Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules
US8785200B2 (en) 1998-11-12 2014-07-22 Life Technologies Corporation Transfection reagents
US9358300B2 (en) 1998-11-12 2016-06-07 Life Technologies Corporation Transfection reagents
US8158827B2 (en) 1998-11-12 2012-04-17 Life Technologies Corporation Transfection reagents
US7915450B2 (en) 1998-11-12 2011-03-29 Life Technologies Corporation Transfection reagents
WO2003014398A2 (en) * 2001-01-03 2003-02-20 Transgenomic, Inc. Methods and compositions for mutation detection by liquid chromatography
WO2003014398A3 (en) * 2001-01-03 2003-07-10 Transgenomic Inc Methods and compositions for mutation detection by liquid chromatography
DE10105208A1 (de) * 2001-02-06 2002-08-14 Ihf Inst Fuer Hormon Und Fortp Verfahren zur Herstellung von cDNA
US6780588B2 (en) 2001-05-07 2004-08-24 Applera Corporation Methods for the reduction of stutter in microsatellite amplification
US6841349B2 (en) 2001-05-07 2005-01-11 Applera Corporation Applied Biosystems Group Methods for the reduction of stutter in microsatellite amplification
US8785126B2 (en) 2001-05-07 2014-07-22 Applied Biosystems, Llc Methods for the reduction of stutter in microsatellite amplification
US7211385B2 (en) 2001-05-07 2007-05-01 Applera Corporation Methods for the reduction of stutter microsatellite amplification
US6783940B2 (en) 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR
EP1452593A4 (en) * 2001-11-14 2005-11-30 Toyo Boseki DNA SYNTHESIS ENGINES, FACTORS ASSOCIATED WITH DNA POLYMERASE, AND USE THEREOF
US7384739B2 (en) 2001-11-14 2008-06-10 Toyo Boseki Kabushiki Kaisha Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof
EP1452593A1 (en) * 2001-11-14 2004-09-01 Toyo Boseki Kabushiki Kaisha Dna synthesis promoters, dna polymerase-associated factors and utilization thereof
WO2004048596A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Methods for using primers that encode one strand of a double-stranded promoter
JPWO2004099446A1 (ja) * 2003-05-07 2006-07-13 タカラバイオ株式会社 遺伝子導入部位の解析方法
EP1621638A1 (en) * 2003-05-07 2006-02-01 Takara Bio Inc. Method of analyzing gene introduction site
JP4496166B2 (ja) * 2003-05-07 2010-07-07 タカラバイオ株式会社 遺伝子導入部位の解析方法
EP1621638A4 (en) * 2003-05-07 2007-05-09 Takara Bio Inc METHOD FOR ANALYZING AN GENERAL ADMINISTRATION CENTER
GB2420560A (en) * 2004-11-30 2006-05-31 Bioline Ltd A method for increasing the specificity of enzymatic DNA synthesis
WO2009006438A2 (en) 2007-06-29 2009-01-08 Epicentre Technologies Corporation Copy dna and sense rna
US10195280B2 (en) 2014-07-15 2019-02-05 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
US10792362B2 (en) 2014-07-15 2020-10-06 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells
US11872285B2 (en) 2014-07-15 2024-01-16 Life Technologies Corporation Compositions and methods for efficient delivery of molecules to cells

Similar Documents

Publication Publication Date Title
DE4411588C1 (de) Puffer für DNA- und RNA-Polymerase-Reaktionen
Røed et al. Microsatellites in reindeer, Rangifer tarandus, and their use in other cervids
DE69432586T2 (de) Methode zum generieren von einzelsträngigen dna molekülen
DE3687287T3 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen.
DE69534728T2 (de) Amplifizierung von langen Nukleinsäuresequenzen mit PCR
DE69736637T2 (de) Multiplex amplifikation kurzer tandem repeat loci
DE69434314T2 (de) Polymorphismus von mononukleotiden und ihre verwendung in der genanalyse
AT402203B (de) Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
ZHANG et al. Highly conserved nuclear copies of the mitochondrial control region in the desert locust Schistocerca gregaria: some implications for population studies
Garner Genome size and microsatellites: the effect of nuclear size on amplification potential
US20140106353A1 (en) Methods And Kits For Multiplex Amplification Of Short Tandem Repeat Loci
DE60212175T2 (de) Verfahren zur reduzierung unspezifischer amplifikation bei pcr
DE112010004821T5 (de) Prozessierung amplifizierter DNA-Fragmente zur Sequenzierung
DE07025195T1 (de) Nukleotidsequenzen des Genoms von Retroviren des Typs HIV-1, HIV-2 und SIV und deren Anwendungen, insbesondere zur Amplifikation von Genomen dieser Retroviren und für die In-Vitro-Diagnostik von durch diese Viren ausgelösten Infektionen
WO2005010209A2 (de) Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren
DE60014350T2 (de) Verfahren zur erkennung und/oder analyse, unter verwendung von primertechniken, von einfachen nukleotidpolymorphismen in restriktionsfragmenten speziell aus amplifizierten restriktionsfragmenten generiert durch aflp
US6767723B2 (en) Method for synthesis of nucleic acids
DE60029225T2 (de) Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese
DE4411594C1 (de) Testkit zur Bestimmung von Histokompatibilitäts-Antigenen
DE69734904T2 (de) ABO-Glycosyltransferase-Sequenz-Polymorphismus
DE69626032T2 (de) Modifizierung von oligonukleotid-primer-sequenzen zum manipilieren der zugabe von nicht-template-nukleotiden
US20070065860A1 (en) Accelerated class I and class II HLA DNA sequence-based typing
DE69833864T2 (de) Methoden zur dns vervielfältigung und material dafür
Klanten et al. Acquiring reef fish DNA sequences from formalin-fixed museum specimens
EP1622940B1 (de) Analyse und verwendung von par 1 polymorphismen zur risikoabschätzung für kardiovaskuläre erkrankungen

Legal Events

Date Code Title Description
8100 Publication of the examined application without publication of unexamined application
D1 Grant (no unexamined application published) patent law 81
8364 No opposition during term of opposition
R071 Expiry of right