DE60040091D1 - Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna-sequenzierungsreaktionen. - Google Patents

Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna-sequenzierungsreaktionen.

Info

Publication number
DE60040091D1
DE60040091D1 DE60040091T DE60040091T DE60040091D1 DE 60040091 D1 DE60040091 D1 DE 60040091D1 DE 60040091 T DE60040091 T DE 60040091T DE 60040091 T DE60040091 T DE 60040091T DE 60040091 D1 DE60040091 D1 DE 60040091D1
Authority
DE
Germany
Prior art keywords
dye
didesoxyterminators
marked
removal
dna sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60040091T
Other languages
English (en)
Inventor
Douglas A Spicer
Karin A Hughes
Robert J Kaiser
James E Mahoney
Amy L Springer
Mark L Stolowitz
Carl H D Weissman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Rockland Inc
Original Assignee
Cambrex Bio Science Rockland Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambrex Bio Science Rockland Inc filed Critical Cambrex Bio Science Rockland Inc
Application granted granted Critical
Publication of DE60040091D1 publication Critical patent/DE60040091D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
DE60040091T 1999-10-06 2000-10-06 Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna-sequenzierungsreaktionen. Expired - Fee Related DE60040091D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US15818899P 1999-10-06 1999-10-06
US16405099P 1999-11-08 1999-11-08
US09/564,117 US6414136B1 (en) 1999-10-06 2000-05-03 Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
PCT/US2000/027895 WO2001025491A1 (en) 1999-10-06 2000-10-06 Removal of dye-labeled dideoxy terminators from dna sequencing reactions

Publications (1)

Publication Number Publication Date
DE60040091D1 true DE60040091D1 (de) 2008-10-09

Family

ID=27388145

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60040091T Expired - Fee Related DE60040091D1 (de) 1999-10-06 2000-10-06 Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna-sequenzierungsreaktionen.

Country Status (10)

Country Link
US (2) US6414136B1 (de)
EP (1) EP1240355B1 (de)
JP (1) JP2003511046A (de)
AT (1) ATE406459T1 (de)
AU (2) AU7872000A (de)
CA (1) CA2386820A1 (de)
DE (1) DE60040091D1 (de)
DK (1) DK1240355T3 (de)
ES (1) ES2310524T3 (de)
WO (2) WO2001025490A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2449445T3 (es) 2000-06-28 2014-03-19 3M Innovative Properties Co. Dispositivos, sistemas y métodos mejorados para el tratamiento de muestras
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
US7075656B2 (en) * 2001-12-11 2006-07-11 Adc Telecommunications, Inc. Method and algorithm for continuous wavelength locking
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US7347976B2 (en) 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US7981600B2 (en) 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US7805228B2 (en) * 2004-08-19 2010-09-28 Spx Corporation Vehicle diagnostic device
FR2886735B1 (fr) * 2005-06-01 2015-09-11 Biomerieux Sa Procede de marquage ou de traitement d'un echantillon biologique contenant des molecules biologiques d'interet, notamment des acides nucleiques
JP5284100B2 (ja) * 2005-11-01 2013-09-11 ノースウエスタン ユニバーシティ マイクロチャネル分離用マトリックス、ダイナミックポリマーシステム及び組成物
JP4919745B2 (ja) * 2006-09-13 2012-04-18 日産自動車株式会社 ナノ粒子及びナノ粒子複合体
KR20090105934A (ko) * 2006-12-22 2009-10-07 쓰리엠 이노베이티브 프로퍼티즈 컴파니 향상된 샘플 처리 장치, 시스템 및 방법
ES2753136T3 (es) 2006-12-22 2020-04-07 Diasorin S P A Métodos de transferencia térmica para sistemas microfluídicos
US20090246762A1 (en) 2008-03-31 2009-10-01 Applera Corporation, Applied Biosystems Group Nucleic acid terminators incorporating a cationic moiety and methods for their use
ES2870874T3 (es) 2011-05-18 2021-10-27 Diasorin S P A Sistemas y métodos para detectar la presencia de un volumen seleccionado de material en un dispositivo de procesamiento de muestra
WO2012158988A1 (en) 2011-05-18 2012-11-22 3M Innovative Properties Company Systems and methods for valving on a sample processing device
ES2755078T3 (es) 2011-05-18 2020-04-21 Diasorin S P A Sistemas y métodos para medición volumétrica en un dispositivo de procesamiento de muestra
WO2013048163A2 (en) * 2011-09-29 2013-04-04 Snu R&Db Foundation Convenient and efficient purification method for chemically labeled nucleic acids
WO2015112914A1 (en) 2014-01-24 2015-07-30 Life Technologies Corporation Purification chemistries and formats for sanger dna sequencing reactions on a micro-fluidics device
GB201700983D0 (en) * 2017-01-20 2017-03-08 Life Tech As Polymeric particles
KR20190028009A (ko) 2017-09-08 2019-03-18 서울대학교산학협력단 pH 제어에 의한 화학적으로 표지된 올리고 핵산의 간편하고 효율적인 분리정제법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
WO1991011533A1 (en) 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5281356A (en) * 1993-03-25 1994-01-25 Lever Brothers Company Heavy duty liquid detergent compositions containing non-proteolytic enzymes comprising capsules comprising proteolytic enzyme and composite polymer
US5564104A (en) 1993-06-08 1996-10-08 Cortex Biochem, Inc. Methods of removing radioactively labled biological molecules from liquid radioactive waste
US6103127A (en) 1993-06-08 2000-08-15 Cortex Biochem, Inc. Methods for removing hazardous organic molecules from liquid waste
US5827531A (en) 1994-12-02 1998-10-27 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Microcapsules and methods for making
US5683875A (en) 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
US5830912A (en) 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US5935572A (en) 1997-01-10 1999-08-10 Collaborative Laboratories, Inc. Composition containing protease separate from glycosidase for removing nits in treating lice infestation

Also Published As

Publication number Publication date
US6818760B1 (en) 2004-11-16
US6414136B1 (en) 2002-07-02
WO2001025491A1 (en) 2001-04-12
EP1240355B1 (de) 2008-08-27
AU8003900A (en) 2001-05-10
EP1240355A4 (de) 2004-12-08
CA2386820A1 (en) 2001-04-12
EP1240355A1 (de) 2002-09-18
ES2310524T3 (es) 2009-01-16
AU7872000A (en) 2001-05-10
ATE406459T1 (de) 2008-09-15
DK1240355T3 (da) 2008-12-08
JP2003511046A (ja) 2003-03-25
WO2001025490A1 (en) 2001-04-12

Similar Documents

Publication Publication Date Title
DE60040091D1 (de) Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna-sequenzierungsreaktionen.
ATE469699T1 (de) Manipulation von mikroteilchen in mikrofluiden systemen
WO2002030561A3 (en) Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
ATE419527T1 (de) Mikrovorrichtung zum screening von biomolekülen
EP1959255A3 (de) Biochemische Analysatoren mit geschlossenem Regelkreis
ATE287765T1 (de) Hybridisierungstest für biomaterial in einem biokanal
ATE268735T1 (de) Herstellung von kombinatorischen arrays von materialen durch methoden der synthese in lösung
HUP0100207A2 (hu) Folyamatos formájú, nagy teljesítményű szűrővizsgálati eljárás
AU3719400A (en) Chemical processing microsystems, diffusion-mixed microreactors and methods for preparing and using same
DE69731939D1 (de) Isolierung von einzelsträngigen nukleinsäuren
DE60127469D1 (de) Potentiometrischer dna-mikroarray, verfahren zu dessen herstellung und verfahren zur nukleinsäureanalyse
DE69827019D1 (de) Gerät und verfahren zur verarbeitung von biologischen zellen
DE69404657T2 (de) Verfahren und vorrichtung zur durchfürung einer vielzahl von chemischen reaktionen auf einer traegerflaeche
DE60321325D1 (de) Mikrokapseln mit kontrollierter durchlässigkeit die ein nukleinsäureamplifizierungsreaktionsgemisch enthalten und deren benutzung als reaktionsgefäss für parallele reaktionen
DE69931085D1 (de) System und Verfahren zum Separieren von biologischen Zellen
DE69809089D1 (de) Verfahren zur Klasssifizierung von hydrophilen Polymerpartikeln und Siebvorrichtung
DE69823372D1 (de) Verfahren und reagentiensatz zur amplifizierung, sequenzierung und typisierung von hla i genen
DE59906395D1 (de) Vorrichtung zur durchführung von chemischen reaktionen
DE602005026412D1 (de) Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten
DE69817818D1 (de) Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle
ATE325190T1 (de) Genetische screeningverfahren
ATE357516T1 (de) Modulation molekularer wechselwirkungspositionen in rns und anderen biomolekülen
ATE414906T1 (de) Verfahren zur identifizierung von an das chaperonin cct bindenden substanzen
DE69840725D1 (de) Methode und reagenzien zum bestimmen von substanzen in biologischen proben
DE59306062D1 (de) Trägermaterial zur simultanen bindung von nukleinsäuren und deren expressionsprodukten

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: LONZA ROCKLAND,INC., ROCKLAND, ME., US

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee