DE60116651D1 - Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren - Google Patents

Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Info

Publication number
DE60116651D1
DE60116651D1 DE60116651T DE60116651T DE60116651D1 DE 60116651 D1 DE60116651 D1 DE 60116651D1 DE 60116651 T DE60116651 T DE 60116651T DE 60116651 T DE60116651 T DE 60116651T DE 60116651 D1 DE60116651 D1 DE 60116651D1
Authority
DE
Germany
Prior art keywords
dna
nucleic acid
acid primer
modified
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60116651T
Other languages
English (en)
Other versions
DE60116651T2 (de
Inventor
Valentine Mccarthy
Ruairi Collins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College Cork
Original Assignee
University College Cork
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University College Cork filed Critical University College Cork
Application granted granted Critical
Publication of DE60116651D1 publication Critical patent/DE60116651D1/de
Publication of DE60116651T2 publication Critical patent/DE60116651T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
DE60116651T 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren Expired - Fee Related DE60116651T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IE20000887 2000-11-03
IE20000887A IE20000887A1 (en) 2000-11-03 2000-11-03 Method for the amplification and optional characterisation of nucleic acids
PCT/IE2001/000139 WO2002036821A2 (en) 2000-11-03 2001-11-01 Method for the amplification and optional characterisation of nucleic acids

Publications (2)

Publication Number Publication Date
DE60116651D1 true DE60116651D1 (de) 2006-04-06
DE60116651T2 DE60116651T2 (de) 2006-09-14

Family

ID=11042688

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60116651T Expired - Fee Related DE60116651T2 (de) 2000-11-03 2001-11-01 Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren

Country Status (13)

Country Link
US (1) US20040067559A1 (de)
EP (1) EP1330546B1 (de)
JP (1) JP2004512843A (de)
CN (1) CN1473201A (de)
AT (1) ATE315666T1 (de)
AU (2) AU1086202A (de)
CA (1) CA2427474A1 (de)
DE (1) DE60116651T2 (de)
DK (1) DK1330546T3 (de)
ES (1) ES2254505T3 (de)
IE (1) IE20000887A1 (de)
RU (1) RU2284357C2 (de)
WO (1) WO2002036821A2 (de)

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US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
EP1828411B1 (de) 2004-12-03 2012-11-07 Human Genetic Signatures PTY Ltd Verfahren zur vereinfachung mikrobieller nukleinsäuren durch chemische modifikation von cytosinen
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
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US8062850B2 (en) 2005-07-25 2011-11-22 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
ATE531820T1 (de) 2005-09-14 2011-11-15 Human Genetic Signatures Pty Gesundheitszustandstest
JP4822801B2 (ja) 2005-10-24 2011-11-24 西川ゴム工業株式会社 変異型エンドヌクレアーゼ
US20070218478A1 (en) * 2005-12-16 2007-09-20 Affymetrix, Inc. Methods for fragmentation and analysis of nucleic acid
US20100173364A1 (en) * 2006-04-11 2010-07-08 New England Biolabs, Inc. Repair of Nucleic Acids for Improved Amplification
WO2008035205A2 (en) 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US9279150B2 (en) 2007-01-10 2016-03-08 General Electric Company Mutant endonuclease V enzymes and applications thereof
US20130323795A1 (en) * 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
CA2680426A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
EP2215250B1 (de) 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzyme zur amplifikation und zum kopieren bisulphit-modifizierter nukleinsäuren
JP2011505845A (ja) * 2007-12-20 2011-03-03 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 核酸増幅に伴う汚染物質の排除
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
CN102296116A (zh) * 2011-09-02 2011-12-28 北京大学 对dna目标序列进行信号放大和检测的方法
NZ622418A (en) 2011-09-07 2014-12-24 Human Genetic Signatures Pty Molecular detection assay
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
AU2014205110A1 (en) * 2013-01-13 2015-08-27 Unitaq Bio Methods and compositions for PCR using blocked and universal primers
US9068218B2 (en) * 2013-01-18 2015-06-30 Emerald Therapeutics, Inc. Rotationally sequestered translators
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP3068883B1 (de) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
SG11201700891SA (en) 2014-08-06 2017-03-30 Nugen Technologies Inc Digital measurements from targeted sequencing
WO2016156071A1 (en) 2015-03-30 2016-10-06 F. Hoffmann-La Roche Ag Methods to amplify highly uniform and less error prone nucleic acid libraries
US10160987B2 (en) 2016-04-07 2018-12-25 Rebecca F. McClure Composition and method for processing DNA
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
KR102520700B1 (ko) * 2018-07-30 2023-04-10 오리시로 제노믹스 가부시키가이샤 무세포계에서 dna를 편집하는 방법
WO2023041931A1 (en) * 2021-09-17 2023-03-23 The University Of Manchester Synthesis of oligonucleotides
CN113897414A (zh) * 2021-10-11 2022-01-07 湖南大地同年生物科技有限公司 一种痕量核酸文库构建方法
CN114369649A (zh) * 2022-02-08 2022-04-19 山东见微生物科技有限公司 一种特异的选择扩增及多重pcr方法和应用

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US5683896A (en) * 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
PT843735E (pt) * 1995-07-11 2002-11-29 Forfas Trading As Biores Irela Deteccao de sequencias de nucleotidos conhecidas mediada por glicosilase
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
CN1198943C (zh) * 1998-04-22 2005-04-27 爱尔兰公司(爱尔兰生物研究) 通过裂解脱碱基位点的核酸产生可延伸的上游dna片段而鉴定核酸分子的方法
US6399309B1 (en) * 2000-12-07 2002-06-04 Becton, Dickinson And Company Amplification and detection of mycoplasma pneumoniae targeting the ORF9 region of the hmw gene cluster

Also Published As

Publication number Publication date
CA2427474A1 (en) 2002-05-10
AU2002210862B2 (en) 2006-06-08
DE60116651T2 (de) 2006-09-14
CN1473201A (zh) 2004-02-04
ATE315666T1 (de) 2006-02-15
AU1086202A (en) 2002-05-15
RU2284357C2 (ru) 2006-09-27
EP1330546A2 (de) 2003-07-30
IE20000887A1 (en) 2002-12-11
ES2254505T3 (es) 2006-06-16
EP1330546B1 (de) 2006-01-11
JP2004512843A (ja) 2004-04-30
WO2002036821A3 (en) 2003-03-27
DK1330546T3 (da) 2006-05-15
WO2002036821A2 (en) 2002-05-10
US20040067559A1 (en) 2004-04-08

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