DE60131194D1 - Sequenzbestimmung in echtzeit - Google Patents

Sequenzbestimmung in echtzeit

Info

Publication number
DE60131194D1
DE60131194D1 DE60131194T DE60131194T DE60131194D1 DE 60131194 D1 DE60131194 D1 DE 60131194D1 DE 60131194 T DE60131194 T DE 60131194T DE 60131194 T DE60131194 T DE 60131194T DE 60131194 D1 DE60131194 D1 DE 60131194D1
Authority
DE
Germany
Prior art keywords
polymerase
tag
tagged
tags
pyrophosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60131194T
Other languages
English (en)
Other versions
DE60131194T2 (de
Inventor
Susan H Hardin
James M Briggs
Shiao-Chun Tu
Xiaolian Gao
Richard Willson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Visigen Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22807697&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60131194(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Visigen Biotechnologies Inc filed Critical Visigen Biotechnologies Inc
Application granted granted Critical
Publication of DE60131194D1 publication Critical patent/DE60131194D1/de
Publication of DE60131194T2 publication Critical patent/DE60131194T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
DE60131194T 2000-07-07 2001-07-09 Sequenzbestimmung in echtzeit Expired - Lifetime DE60131194T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21659400P 2000-07-07 2000-07-07
US216594P 2000-07-07
PCT/US2001/021811 WO2002004680A2 (en) 2000-07-07 2001-07-09 Real-time sequence determination

Publications (2)

Publication Number Publication Date
DE60131194D1 true DE60131194D1 (de) 2007-12-13
DE60131194T2 DE60131194T2 (de) 2008-08-07

Family

ID=22807697

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60131194T Expired - Lifetime DE60131194T2 (de) 2000-07-07 2001-07-09 Sequenzbestimmung in echtzeit

Country Status (9)

Country Link
US (22) US20030064366A1 (de)
EP (3) EP2100971A3 (de)
JP (3) JP2004513619A (de)
CN (2) CN101525660A (de)
AT (1) ATE377093T1 (de)
AU (2) AU2001282881B2 (de)
CA (1) CA2415897A1 (de)
DE (1) DE60131194T2 (de)
WO (1) WO2002004680A2 (de)

Families Citing this family (675)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9213691D0 (en) * 1992-06-27 1992-08-12 Hurlstone Gary Personal alarm torch
US5823720A (en) * 1996-02-16 1998-10-20 Bitmoore High precision cutting tools
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
DE69930310T3 (de) 1998-12-14 2009-12-17 Pacific Biosciences of California, Inc. (n. d. Ges. d. Staates Delaware), Menlo Park Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
CN101525660A (zh) * 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
CA2440754A1 (en) * 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7727722B2 (en) * 2001-08-29 2010-06-01 General Electric Company Ligation amplification
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US20050009124A1 (en) * 2001-11-26 2005-01-13 Echelon Biosciences Incorporated Assays for detection of phosphoinositide kinase and phosphatase activity
EP1497304B1 (de) * 2002-04-12 2014-06-25 Catalyst Assets LLC Doppelt markierte nukleotide
EP1546399B1 (de) * 2002-08-29 2012-02-29 GE Healthcare Bio-Sciences Corp. Nukleosidpolyphosphate mit blockiertem terminalem phosphat
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
ATE522620T1 (de) * 2002-11-14 2011-09-15 Wayne John Cancer Inst Nachweis von micrometastasierung von melanomen und brustkrebs an in paraffin eingegbetteten, von tumoren abführenden lymphknoten mittels quantitativer multimaker rt-pcr
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
EP1592779A4 (de) * 2003-02-05 2007-12-12 Ge Healthcare Bio Sciences Phosphat-endmarkierte nukleotide mit neuen linkern
AU2003265857A1 (en) * 2003-02-05 2004-09-06 Amersham Biosciences Corp Nucleic acid amplification
WO2004071948A2 (en) * 2003-02-10 2004-08-26 Reveo, Inc. Micro-nozzle, nano-nozzle, manufacturing methods therefor, applications therefor
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US7833709B2 (en) 2004-05-28 2010-11-16 Wafergen, Inc. Thermo-controllable chips for multiplex analyses
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
JP2008513782A (ja) * 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子解析のための装置及び方法
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7405434B2 (en) * 2004-11-16 2008-07-29 Cornell Research Foundation, Inc. Quantum dot conjugates in a sub-micrometer fluidic channel
JP2006197832A (ja) * 2005-01-19 2006-08-03 Tohoku Univ 環境感受性蛍光プローブを用いたシステイン残基が変異導入されたabcトランスポーターの汎用的な基質親和性検出方法
WO2006086673A2 (en) * 2005-02-09 2006-08-17 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof
EP1901057A1 (de) * 2005-07-07 2008-03-19 Sony Corporation Verfahren zur erfassung von substanzdaten, vorrichtung zur messung von substanzdaten mittels evaneszenzlicht sowie entscheidungsverfahren und -vorrichtung für eine basisanordnung
GB0514935D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070190542A1 (en) * 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
US10060904B1 (en) 2005-10-17 2018-08-28 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US7825037B2 (en) * 2005-10-17 2010-11-02 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US9156004B2 (en) 2005-10-17 2015-10-13 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US8017331B2 (en) 2005-11-04 2011-09-13 Mannkind Corporation IRE-1α substrates
WO2007070572A2 (en) * 2005-12-12 2007-06-21 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Probe for nucleic acid sequencing and methods of use
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) * 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CA2672315A1 (en) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US20100035268A1 (en) * 2007-02-21 2010-02-11 Joseph Beechem Materials and methods for single molecule nucleic acid sequencing
US9121843B2 (en) 2007-05-08 2015-09-01 Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
WO2008147879A1 (en) * 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8703422B2 (en) 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US8182993B2 (en) * 2007-06-06 2012-05-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
EP2201136B1 (de) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Sequenzierung mittels nanoporen und hybridisierung von sonden zur bildung ternärer komplexe und ausrichtung mit variablem bereich
JP2011505119A (ja) * 2007-10-22 2011-02-24 ライフ テクノロジーズ コーポレーション 核酸分子に沿って順序付けられ分断化された配列断片を得るための方法およびシステム
GB0721340D0 (en) * 2007-10-30 2007-12-12 Isis Innovation Polymerase-based single-molecule DNA sequencing
WO2009117031A2 (en) * 2007-12-18 2009-09-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
CN101519698B (zh) * 2008-03-10 2013-05-22 周国华 一种利用序列标签定量测定核酸的方法
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
JP4586081B2 (ja) * 2008-03-31 2010-11-24 株式会社日立ハイテクノロジーズ 蛍光分析装置
US20090269746A1 (en) * 2008-04-25 2009-10-29 Gil Atzmon Microsequencer-whole genome sequencer
MX2010013678A (es) 2008-06-11 2011-02-24 Lasergen Inc Nucleotidos y nucleosidos y metodos para su uso en secuenciacion de adn.
JP5268444B2 (ja) * 2008-06-23 2013-08-21 株式会社日立ハイテクノロジーズ 単分子リアルタイムシーケンス装置,核酸分析装置及び単分子リアルタイムシーケンス方法
US8470164B2 (en) 2008-06-25 2013-06-25 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20110281740A1 (en) * 2008-06-30 2011-11-17 Joseph Beechem Methods for Real Time Single Molecule Sequencing
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100035252A1 (en) * 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
CN102186989B (zh) * 2008-09-03 2021-06-29 纳伯塞斯2.0有限责任公司 用于流体通道中生物分子和其它分析物的电压感测的纵向移位纳米级电极的使用
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010039189A2 (en) * 2008-09-23 2010-04-08 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8486865B2 (en) * 2008-11-03 2013-07-16 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
EP2379748A4 (de) * 2008-12-23 2012-08-29 Illumina Inc Multibase-freisetzung für lange auslesevorgänge bei squenzierungen mittels syntheseprotokollen
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
WO2010111691A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Conjugates of biomolecules to nanoparticles
WO2010111605A2 (en) * 2009-03-27 2010-09-30 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
US8501405B2 (en) * 2009-04-27 2013-08-06 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
WO2010127304A2 (en) * 2009-05-01 2010-11-04 Illumina, Inc. Sequencing methods
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
WO2010144151A2 (en) * 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Single-molecule real-time analysis of protein synthesis
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
CN102625838B (zh) * 2009-08-14 2016-01-20 阿霹震中科技公司 用于生成rRNA除尽的样本或者用于从样本分离rRNA的方法、组合物和试剂盒
WO2011028296A2 (en) * 2009-09-07 2011-03-10 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
WO2011078897A1 (en) * 2009-09-15 2011-06-30 Life Technologies Corporation Improved sequencing methods, compositions, systems, kits and apparatuses
WO2011040996A1 (en) 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
EP2957641B1 (de) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple-displacement-amplifikation
EP2507387B1 (de) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Vorrichtung und verfahren für biochemische analyse
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
EP2519823B1 (de) * 2009-12-28 2022-05-18 The United States Of America, As Represented By The Secretary, Department of Health and Human Services Verbundsonden und deren verwendung in hochauflösungsverfahren
ES2596655T3 (es) 2010-02-01 2017-01-11 Illumina Inc. Procedimientos de enfoque y sistemas y conjuntos ópticos que usan los mismos
US8603741B2 (en) * 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8481903B2 (en) * 2010-03-06 2013-07-09 Alexander Triener Systems, methods, and apparatuses including a moveable optical component for detecting optical signals from a sample
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
JP5893607B2 (ja) 2010-04-05 2016-03-23 プログノシス バイオサイエンシズ インコーポレイテッドPrognosys Biosciences,Inc. 空間コード化生物学的アッセイ
AU2011249913B2 (en) 2010-05-06 2014-09-11 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
JP5952813B2 (ja) 2010-06-30 2016-07-13 ライフ テクノロジーズ コーポレーション Isfetアレイをテストする方法及び装置
US8731847B2 (en) 2010-06-30 2014-05-20 Life Technologies Corporation Array configuration and readout scheme
AU2011226767B1 (en) 2010-06-30 2011-11-10 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
EP2589065B1 (de) 2010-07-03 2015-08-19 Life Technologies Corporation Chemisch empfindlicher sensor mit leicht dotierten abflüssen
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
WO2012027625A2 (en) 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Scaffold-based polymerase enzyme substrates
WO2012036679A1 (en) 2010-09-15 2012-03-22 Life Technologies Corporation Methods and apparatus for measuring analytes
US9938590B2 (en) 2010-09-16 2018-04-10 Gen-Probe Incorporated Capture probes immobilizable via L-nucleotide tail
AU2011301935B2 (en) 2010-09-16 2015-06-11 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
EP2619564B1 (de) 2010-09-24 2016-03-16 Life Technologies Corporation Transistorschaltungen mit zusammengehörigen paaren
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
EP2633069B1 (de) 2010-10-26 2015-07-01 Illumina, Inc. Sequenzierungsverfahren
US9255293B2 (en) 2010-11-01 2016-02-09 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (de) 2010-11-05 2017-04-19 Illumina, Inc. Verknüpfung von sequenzstücken mit gepaarten code-tags
EP2640849B1 (de) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Verfahren zur sequenzierung von biomolekülen durch nachweis der relativen positionen von hybridisierten sonden
EP2643484A4 (de) 2010-11-22 2014-04-16 Univ California Verfahren zur identifizierung eines rna-transkripts zellulären ursprungs
CN105755545B (zh) 2010-12-27 2019-05-03 艾比斯生物科学公司 核酸样品的制备方法及组合物
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
WO2012106081A2 (en) 2011-01-31 2012-08-09 Illumina, Inc. Methods for reducing nucleic acid damage
WO2012104851A1 (en) 2011-01-31 2012-08-09 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
WO2012109315A1 (en) * 2011-02-08 2012-08-16 Life Technologies Corporation Linking methods, compositions, systems, kits and apparatuses
WO2012109574A2 (en) 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
ES2538694T3 (es) * 2011-04-01 2015-06-23 F. Hoffmann-La Roche Ag Variantes de la T7 ARN polimerasa con sustituciones de Cisteína-Serina
WO2012139110A2 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
EP3293274B1 (de) 2011-08-10 2020-04-01 Life Technologies Corporation Polymerasezusammensetzungen
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
EP3670523B1 (de) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh-unblockierte, schnell photospaltbare endgruppennukleotide und ihre verwendung in nukleinsäuresequenzierungsverfahren
AU2012316218B2 (en) 2011-09-26 2016-03-17 Gen-Probe Incorporated Algorithms for sequence determinations
US9164053B2 (en) * 2011-09-26 2015-10-20 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
WO2013063308A1 (en) 2011-10-25 2013-05-02 University Of Massachusetts An enzymatic method to enrich for capped rna, kits for performing same, and compositions derived therefrom
WO2013063382A2 (en) 2011-10-28 2013-05-02 Illumina, Inc. Microarray fabrication system and method
JP5863396B2 (ja) * 2011-11-04 2016-02-16 株式会社日立製作所 Dna配列解読システム、dna配列解読方法及びプログラム
EP2776165A2 (de) 2011-11-07 2014-09-17 Illumina, Inc. Integrierte sequenzierungsvorrichtungen und verwendungsverfahren
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
EP2788499B1 (de) 2011-12-09 2016-01-13 Illumina, Inc. Expandierte wurzel für polymer-tags
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
US10150993B2 (en) 2011-12-22 2018-12-11 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
EP2794927B1 (de) 2011-12-22 2017-04-12 Ibis Biosciences, Inc. Amplifikationsprimer und verfahren
EP3269820A1 (de) 2011-12-22 2018-01-17 Ibis Biosciences, Inc. Kit für die amplifizierung einer sequenz aus einer ribonukleinsäure
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
CA2863121A1 (en) 2011-12-30 2013-07-04 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
JP2015503923A (ja) 2012-01-09 2015-02-05 オスロ ウニヴェルスィテーツスィーケフース ハーエフOslo Universitetssykehus Hf 結腸直腸癌の解析のための方法およびバイオマーカー
WO2013106737A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
WO2013116774A1 (en) 2012-02-01 2013-08-08 Gen-Probe Incorporated Asymmetric hairpin target capture oligomers
US8838394B2 (en) * 2012-02-03 2014-09-16 California Institute Of Technology Signal encoding and decoding in multiplexed biochemical assays
EP2820174B1 (de) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Verfahren und verwendung für molekulare tags
ES2663234T3 (es) 2012-02-27 2018-04-11 Cellular Research, Inc Composiciones y kits para recuento molecular
NO2694769T3 (de) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
AU2013243998B2 (en) 2012-04-03 2016-07-28 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
EP2834370B1 (de) 2012-04-03 2019-01-02 The Regents Of The University Of Michigan Biomarker im zusammenhang mit dem reizdarmsyndrom und morbus crohn
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
EP3438285B1 (de) 2012-05-02 2020-10-14 Ibis Biosciences, Inc. Dna-sequenzierung
ES2683707T3 (es) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. Secuenciación de ADN
EP2844774B1 (de) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna-sequenzierung
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
EP2850086B1 (de) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyaninfarbstoffe
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
EP2870264A4 (de) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative beurteilung der wiederherstellung eines menschlichen t-zellen-repertoires nach der transplantation allogener blutbildender stammzellen
CA2878280A1 (en) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US10895534B2 (en) 2012-08-20 2021-01-19 Illumina, Inc. Method and system for fluorescence lifetime based sequencing
ES2701750T3 (es) 2012-10-16 2019-02-25 Abbott Molecular Inc Procedimientos para secuenciar un ácido nucleico
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10557133B2 (en) 2013-03-13 2020-02-11 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
CA2898453C (en) 2013-03-13 2021-07-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
US9701999B2 (en) 2013-03-14 2017-07-11 Abbott Molecular, Inc. Multiplex methylation-specific amplification systems and methods
WO2014142921A1 (en) 2013-03-14 2014-09-18 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
EP2971154A4 (de) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nukleinsäurekontrollpanels
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
CN105358709B (zh) 2013-03-15 2018-12-07 雅培分子公司 用于检测基因组拷贝数变化的系统和方法
CN105051525B (zh) 2013-03-15 2019-07-26 生命科技公司 具有薄导电元件的化学设备
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
CN105264366B (zh) 2013-03-15 2019-04-16 生命科技公司 具有一致传感器表面区域的化学传感器
EP3533884A1 (de) 2013-03-15 2019-09-04 Ibis Biosciences, Inc. Dna-sequenzen zur beurteilung der kontamination in der dna-sequenzierung
DK2970356T3 (en) 2013-03-15 2018-08-27 Illumina Cambridge Ltd Modified nucleosides or nucleotides
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
CN105283560B (zh) 2013-05-24 2018-11-30 昆塔波尔公司 基于纳米孔的通过混合的fret检测的核酸分析
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
PL3017065T3 (pl) 2013-07-01 2019-03-29 Illumina, Inc. Funkcjonalizacja powierzchni i szczepienie polimeru bez użycia katalizatora
EP3241913B1 (de) 2013-07-03 2019-02-20 Illumina, Inc. System zur sequenzierung durch orthogonale synthese
CN103333680B (zh) * 2013-07-16 2015-05-27 北京化工大学 一种具有多色荧光特性的二苯基恶唑共晶材料及其制备方法
CA2915875C (en) 2013-08-08 2018-09-18 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
EP3036359B1 (de) 2013-08-19 2019-10-23 Abbott Molecular Inc. Sequenzierungsbibliotheken der nächsten generation
EP3039158B1 (de) 2013-08-28 2018-11-14 Cellular Research, Inc. Massiv parallele einzelzellanalyse
CN105916689A (zh) 2013-08-30 2016-08-31 Illumina公司 在亲水性或斑驳亲水性表面上的微滴操纵
WO2015042708A1 (en) 2013-09-25 2015-04-02 Bio-Id Diagnostic Inc. Methods for detecting nucleic acid fragments
US9657281B2 (en) 2013-09-30 2017-05-23 Life Technologies Corporation Polymerase compositions, methods of making and using same
JP2017504307A (ja) 2013-10-07 2017-02-09 セルラー リサーチ, インコーポレイテッド アレイ上のフィーチャーをデジタルカウントするための方法およびシステム
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
EP3940082A1 (de) 2013-12-03 2022-01-19 Illumina, Inc. Verfahren und systeme zur analyse von bilddaten
SG10201804913YA (en) 2013-12-10 2018-07-30 Illumina Inc Biosensors for biological or chemical analysis and methods of manufacturing the same
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
AU2014364926B2 (en) 2013-12-20 2017-12-14 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
ES2848062T3 (es) 2013-12-23 2021-08-05 Illumina Inc Sustratos estructurados para mejorar la detección de emisiones de luz y métodos relacionados con los mismos
AU2014377537B2 (en) 2014-01-16 2021-02-25 Illumina, Inc. Amplicon preparation and sequencing on solid supports
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015109234A1 (en) * 2014-01-16 2015-07-23 Illumina, Inc. Gene expression panel for prognosis of prostate cancer recurrence
SI3108009T1 (sl) 2014-02-18 2021-09-30 Illumina, Inc. Postopki in sestavki za profiliranje DNK
EP3698874A1 (de) 2014-03-11 2020-08-26 Illumina, Inc. Wegwerfbare, integrierte mikrofluidische kartusche und verfahren zur herstellung davon
EP3680333A1 (de) 2014-04-29 2020-07-15 Illumina, Inc. Multiplexierte einzelzellengenexpressionsanalyse mittels vorlagenwechsel und tagmentation
CA3219413A1 (en) 2014-05-16 2015-11-19 Illumina, Inc. Nucleic acid synthesis techniques
EP3148697A1 (de) 2014-05-27 2017-04-05 Illumina, Inc. Systeme und verfahren zur biochemischen analyse mit einem basisinstrument und einer auswechselbaren kassette
DK3152320T3 (da) 2014-06-03 2021-01-11 Illumina Inc Sammensætninger, systemer og fremgangsmåder til at detektere begivenheder med anvendelse af tethere, der er forankret til eller hosliggende til nanoporer
US10760109B2 (en) 2014-06-06 2020-09-01 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
CN106715693A (zh) 2014-06-13 2017-05-24 伊卢米纳剑桥有限公司 用于制备序列文库的方法和组合物
WO2015200378A1 (en) 2014-06-23 2015-12-30 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2015200693A1 (en) 2014-06-27 2015-12-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
BR112016030008A2 (pt) 2014-06-27 2017-10-24 Abbott Lab método para a detecção de infecção por pegivirus humano 2 em um indivíduo, para a detecção de ácido nucleico de pegivirus humano 2 e para a detecção de pegivirus humano 2 em uma amostra, e, composição
AU2015284464B2 (en) 2014-06-30 2021-06-17 Illumina, Inc. Methods and compositions using one-sided transposition
EP3828279B1 (de) 2014-07-15 2022-11-16 Illumina, Inc. Biochemisch aktivierte elektronische vorrichtung
AU2015294354B2 (en) 2014-07-21 2021-10-28 Illumina, Inc. Polynucleotide enrichment using CRISPR-Cas systems
MX2017001134A (es) 2014-07-24 2017-10-11 Abbott Molecular Inc Composiciones y metodos para la deteccion y analisis de tuberculosis micobacteriana.
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
US9982250B2 (en) 2014-08-21 2018-05-29 Illumina Cambridge Limited Reversible surface functionalization
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
EP3191606B1 (de) 2014-09-12 2020-05-27 Illumina, Inc. Verfahren zur erkennung der anwesenheit von polymeruntereinheiten mit chemilumineszenz
AU2015318017B2 (en) 2014-09-18 2022-02-03 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
EP3201355B1 (de) 2014-09-30 2019-07-31 Illumina, Inc. Modifizierte polymerasen zur verbesserten inkorporation von nukleotidanaloga
WO2016153999A1 (en) 2015-03-25 2016-09-29 Life Technologies Corporation Modified nucleotides and uses thereof
CA2960721C (en) 2014-10-09 2023-09-05 Illumina, Inc. Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
CA2963604C (en) 2014-10-10 2023-02-14 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
SG11201703139VA (en) 2014-10-17 2017-07-28 Illumina Cambridge Ltd Contiguity preserving transposition
CA2964790C (en) 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
PT3212684T (pt) 2014-10-31 2020-02-03 Illumina Cambridge Ltd Revestimentos de polímeros e de copolímero de dna
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
WO2016073237A1 (en) 2014-11-05 2016-05-12 Illumina Cambridge Limited Reducing dna damage during sample preparation and sequencing using siderophore chelators
AU2015346514B2 (en) 2014-11-11 2021-04-08 Illumina, Inc. Polynucleotide amplification using CRISPR-Cas systems
CN107208019B (zh) 2014-11-11 2021-01-01 伊鲁米纳剑桥有限公司 用于核酸单克隆簇的产生和测序的方法和阵列
RU2582198C1 (ru) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии
CN104458686B (zh) * 2014-12-02 2017-01-18 公安部第一研究所 一种基于特征分子量内标定量分析的dna荧光光谱采集方法
US10350570B2 (en) 2014-12-15 2019-07-16 Illumina, Inc. Compositions and methods for single molecular placement on a substrate
CN107208074B (zh) 2014-12-16 2021-06-15 生命技术公司 聚合酶组合物和制造与使用其的方法
EP3234575B1 (de) 2014-12-18 2023-01-25 Life Technologies Corporation Vorrichtung zur messung von analyten unter verwendung von grossflächigen fet-arrays
KR102593647B1 (ko) 2014-12-18 2023-10-26 라이프 테크놀로지스 코포레이션 트랜스미터 구성을 갖춘 높은 데이터율 집적 회로
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
US11634707B2 (en) 2015-02-10 2023-04-25 Illumina, Inc. Methods and compositions for analyzing cellular components
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
EP3822361A1 (de) 2015-02-20 2021-05-19 Takara Bio USA, Inc. Verfahren zur schnellen präzisen abgabe, visualisierung und analyse von einzelzellen
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016154038A1 (en) 2015-03-20 2016-09-29 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
EP4089398A1 (de) 2015-03-24 2022-11-16 Illumina, Inc. Trägervorrichtungen und systeme zum abbilden von proben zur biologischen oder chemischen analyse
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
US20180100192A1 (en) 2015-03-31 2018-04-12 lllumina Cambridge Limited Surface concatemerization of templates
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10900030B2 (en) 2015-04-14 2021-01-26 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
JP2018518155A (ja) 2015-04-15 2018-07-12 ザ ジェネラル ホスピタル コーポレイション Lnaベース突然変異体エンリッチメント次世代シーケンシングアッセイ
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
DK3294911T3 (da) 2015-05-11 2020-11-16 Illumina Inc Platform til opdagelse og analyse af terapeutiske midler
WO2016196210A2 (en) 2015-05-29 2016-12-08 Illumina, Inc. Sample carrier and assay system for conducting designated reactions
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
WO2016193695A1 (en) 2015-05-29 2016-12-08 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
IL293410B2 (en) 2015-06-03 2024-01-01 Illumina Inc Compositions, systems and methods for sequencing polynucleotides using tethers anchored to polymerases attached to nanopores
DK3320111T3 (da) 2015-07-06 2021-07-19 Illumina Cambridge Ltd Prøveklargøring til nukleinsyreamplifikation
GB2556713B (en) 2015-07-06 2021-06-23 Illumina Inc Balanced AC modulation for driving droplet operations electrodes
EP3320399A1 (de) 2015-07-07 2018-05-16 Illumina, Inc. Selektive oberflächenmustererzeugung mittels nanoprägung
US10526664B2 (en) 2015-07-14 2020-01-07 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
US10392613B2 (en) 2015-07-14 2019-08-27 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
US20180207920A1 (en) 2015-07-17 2018-07-26 Illumina, Inc. Polymer sheets for sequencing applications
WO2017014762A1 (en) * 2015-07-21 2017-01-26 Omniome, Inc. Nucleic acid sequencing methods and systems
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
US11155864B2 (en) 2015-07-30 2021-10-26 Illumina, Inc. Orthogonal deblocking of nucleotides
EP3854884A1 (de) 2015-08-14 2021-07-28 Illumina, Inc. Systeme und verfahren mit magnetempfindlichen sensoren zur bestimmung eines genetischen merkmals
WO2017034868A1 (en) 2015-08-24 2017-03-02 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
CA3172078A1 (en) 2015-08-28 2017-03-09 Illumina, Inc. Nucleic acid sequence analysis from single cells
US10906044B2 (en) 2015-09-02 2021-02-02 Illumina Cambridge Limited Methods of improving droplet operations in fluidic systems with a filler fluid including a surface regenerative silane
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
AU2016319110B2 (en) 2015-09-11 2022-01-27 The General Hospital Corporation Full interrogation of nuclease DSBs and sequencing (FIND-seq)
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
JP2018529353A (ja) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション 配列決定法による切断事象の包括的生体外報告(CIRCLE−seq)
EP3356557B1 (de) 2015-10-01 2022-07-20 Life Technologies Corporation Polymerasezusammensetzungen und kits sowie verfahren zur verwendung und herstellung davon
JP6936222B2 (ja) 2015-10-22 2021-09-15 イラミーナ インコーポレーテッド 流体装置のための充填剤流体
WO2017095917A1 (en) 2015-12-01 2017-06-08 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
WO2017106481A1 (en) 2015-12-17 2017-06-22 Illumina, Inc. Distinguishing methylation levels in complex biological samples
CN108431223A (zh) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 每液滴分辨率下的多个珠
TW201730563A (zh) 2016-01-11 2017-09-01 伊路米納有限公司 具有顯微螢光計、流體系統和流動單元閂鎖夾持模組的檢測裝置
WO2017139260A1 (en) 2016-02-08 2017-08-17 RGENE, Inc. Multiple ligase compositions, systems, and methods
EP3430154B1 (de) 2016-03-14 2020-11-11 Rgene, Inc. Hyperthermostabile lysin-mutante ssdna/rna-ligasen
CN108474744B (zh) 2016-03-24 2019-11-05 伊鲁米那股份有限公司 在发光成像中使用的基于光子超晶格的设备和组成物及其使用方法
ES2861350T3 (es) 2016-03-28 2021-10-06 Illumina Inc Micromatrices de planos múltiples
ES2786974T3 (es) 2016-04-07 2020-10-14 Illumina Inc Métodos y sistemas para la construcción de bibliotecas de ácidos nucleicos normalizadas
WO2017177017A1 (en) 2016-04-07 2017-10-12 Omniome, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
CN109072297B (zh) 2016-04-22 2022-09-13 加利福尼亚太平洋生物科学股份有限公司 使用增强的核苷酸特异性三元复合物形成检测的核酸测序方法和系统
KR102356022B1 (ko) 2016-04-22 2022-01-27 일루미나, 인코포레이티드 픽셀 내의 복수의 부위의 발광 이미징에서 사용하기 위한 광자 구조체-기반 디바이스 및 조성물, 및 이를 사용하는 방법
US10294514B2 (en) 2016-04-29 2019-05-21 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
WO2017197027A1 (en) 2016-05-11 2017-11-16 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
KR102171865B1 (ko) 2016-05-18 2020-10-29 일루미나, 인코포레이티드 패턴화된 소수성 표면을 사용한 자가 조립된 패터닝
BR112018073378A2 (pt) * 2016-05-20 2019-03-06 Quantum-Si Incorporated composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
WO2018009346A1 (en) 2016-07-05 2018-01-11 Quantapore, Inc. Optically based nanopore sequencing
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
WO2018013558A1 (en) 2016-07-12 2018-01-18 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
CA3020629A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
KR20230003255A (ko) 2016-07-22 2023-01-05 오레곤 헬스 앤드 사이언스 유니버시티 단일 세포 전체 게놈 라이브러리 및 이의 제조를 위한 조합 인덱싱 방법
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
WO2018035134A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Method and system for sequencing nucleic acids
WO2018042251A1 (en) 2016-08-29 2018-03-08 Oslo Universitetssykehus Hf Chip-seq assays
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
US20180127816A1 (en) 2016-10-19 2018-05-10 Illumina, Inc. Methods for chemical ligation of nucleic acids
SG11201903158RA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for cell label classification
EP3539035B1 (de) 2016-11-08 2024-04-17 Becton, Dickinson and Company Verfahren zur klassifizierung von expressionsprofilen
BR112019009949A2 (pt) 2016-11-16 2019-08-20 Illumina Inc método implantado por computador para validar chamadas de variante e sistema para validar chamadas de variante
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
PL3551753T3 (pl) 2016-12-09 2022-10-31 The Broad Institute, Inc. Diagnostyka oparta na uk‎‎‎ładzie efektorowym crispr
EP3555290B1 (de) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Markierte dna mit erhaltener tröpfchenmarkierungskontinuität
RU2765075C2 (ru) 2016-12-22 2022-01-25 Иллюмина, Инк. Биочип, включающий праймер секвенирования и несеквенирующую единицу
EP3559262A4 (de) 2016-12-22 2020-07-22 Illumina, Inc. Arrays mit qualitätskontroll-tracern
KR102512186B1 (ko) 2016-12-22 2023-03-20 일루미나, 인코포레이티드 수지 필름 및 패턴화된 중합체층을 포함하는 어레이
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法
AU2017386515B2 (en) 2016-12-30 2021-08-19 Pacific Biosciences Of California, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
HUE058858T2 (hu) 2017-01-06 2022-09-28 Illumina Inc Fáziskorrekció
EP3568490B1 (de) 2017-01-10 2021-03-10 Omniome, Inc. Manipulierte polymerasen zur reduzierung von nukleotidunabhängiger dna-bindung
EP3568234B1 (de) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophile beschichtung von fluidischen kanälen
CA3045498C (en) 2017-01-17 2021-07-13 Illumina, Inc. Oncogenic splice variant determination
CN110313034B (zh) 2017-01-18 2023-06-06 伊鲁米那股份有限公司 对核酸分子进行测序的方法、机器可读介质和计算机系统
EP3571319A1 (de) 2017-01-20 2019-11-27 Omniome, Inc. Verfahren zur detektion verwandter nukleotide in einem nukleinsäuresequenzierungaarbeitsablauf
EP3565900B1 (de) 2017-01-20 2021-04-07 Omniome, Inc. Genotypisierung durch polymerasebindung
WO2018136117A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Allele-specific capture of nucleic acids
DK3354746T3 (da) 2017-01-30 2019-09-02 Gmi Gregor Mendel Inst Fuer Molekulare Pflanzenbiologie Gmbh Nye spike-in-nukleotider til normalisering af sekvensdata
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
JP7164276B2 (ja) 2017-02-21 2022-11-01 イルミナ インコーポレイテッド リンカーを用いた固定化トランスポソームを使用するタグメンテーション
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11021740B2 (en) 2017-03-15 2021-06-01 The Broad Institute, Inc. Devices for CRISPR effector system based diagnostics
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
US20200181720A1 (en) 2017-03-15 2020-06-11 The Broad Institute, Inc. Crispr effector system based diagnostics for virus detection
CA3056428A1 (en) 2017-03-20 2018-09-27 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
US10737267B2 (en) 2017-04-04 2020-08-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
DK3872187T3 (da) 2017-04-23 2022-12-05 Illumina Cambridge Ltd Sammensætninger og fremgangsmåder til forbedring af prøveidentificering i indekserede nukleinsyrebiblioteker
EP3913053A1 (de) 2017-04-23 2021-11-24 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
CA3059952C (en) 2017-04-23 2023-04-18 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CN110799653A (zh) 2017-05-01 2020-02-14 伊鲁米那股份有限公司 用于多重大规模平行测序的最佳索引序列
SG11201910070PA (en) 2017-05-08 2019-11-28 Illumina Inc Universal short adapters for indexing of polynucleotide samples
JP2020522262A (ja) 2017-06-05 2020-07-30 ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company 単一細胞用のサンプルインデックス付加
KR102628035B1 (ko) 2017-06-07 2024-01-22 오레곤 헬스 앤드 사이언스 유니버시티 메틸화 서열결정을 위한 단일 세포 전체 게놈 라이브러리
WO2018227091A1 (en) 2017-06-08 2018-12-13 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
US11702654B2 (en) 2017-06-20 2023-07-18 Illumina, Inc. Methods and compositions for addressing inefficiencies in amplification reactions
CN110770356A (zh) 2017-06-20 2020-02-07 生物辐射实验室股份有限公司 使用珠寡核苷酸的mda
WO2019002265A1 (en) 2017-06-26 2019-01-03 Universität Für Bodenkultur Wien NOVEL BIOMARKERS FOR THE DETECTION OF SENESCENT CELLS
WO2019018366A1 (en) 2017-07-18 2019-01-24 Omniome, Inc. METHOD OF CHEMICAL MODIFICATION OF PLASTIC SURFACES
JP7287942B2 (ja) 2017-07-24 2023-06-06 クアンタム-エスアイ インコーポレイテッド 高強度標識反応物組成物および配列決定のための方法
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
EP3545106B1 (de) 2017-08-01 2022-01-19 Helitec Limited Verfahren zur anreicherung und bestimmung von zielnukleotidsequenzen
AU2018312560B2 (en) 2017-08-01 2022-03-10 Illumina, Inc. Hydrogel beads for nucleotide sequencing
EP4289967A3 (de) 2017-08-01 2024-03-20 Illumina, Inc. Räumliche indexierung von genetischem material und bibliothekherstellung unter verwendung von hydrogel-kügelchen und durchflusszellen
WO2019035897A1 (en) 2017-08-15 2019-02-21 Omniome, Inc. SCANNING APPARATUS AND METHODS USEFUL FOR DETECTION OF CHEMICAL OR BIOLOGICAL ANALYTES
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
KR20200057024A (ko) 2017-09-20 2020-05-25 가던트 헬쓰, 인크. 체세포 및 생식세포계열 변이체를 구별하기 위한 방법 및 시스템
US11837324B2 (en) 2017-10-16 2023-12-05 Illumina, Inc. Deep learning-based aberrant splicing detection
BR112019027480B1 (pt) 2017-10-16 2022-04-19 Illumina, Inc Métodos para a construção de um classificador de patogenicidade variante, meio de armazenamento legível por computador não transitório impresso, e sistema
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
EP4180534A1 (de) 2017-11-02 2023-05-17 Bio-Rad Laboratories, Inc. Auf transposase basierende genomanalyse
EP3728636A1 (de) 2017-12-19 2020-10-28 Becton, Dickinson and Company Mit oligonukleotiden assoziierte teilchen
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
AU2019205797A1 (en) 2018-01-08 2019-12-19 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
CN110832510A (zh) 2018-01-15 2020-02-21 因美纳有限公司 基于深度学习的变体分类器
US20210371926A1 (en) 2018-01-29 2021-12-02 The Broad Institute, Inc. Crispr effector system based diagnostics
EP4324962A2 (de) 2018-01-31 2024-02-21 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur dekonvolution von partitionsbarcodes
KR20200139671A (ko) 2018-02-06 2020-12-14 옴니옴 인코포레이티드 핵산 프라이머 연장을 위한 조성물 및 기술
EP3752634B8 (de) 2018-02-13 2023-10-11 Illumina, Inc. Dna-sequenzierung unter verwendung von hydrogelperlen
US20190367909A1 (en) 2018-04-02 2019-12-05 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
WO2019200338A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant classifier based on deep neural networks
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
WO2019204229A1 (en) 2018-04-20 2019-10-24 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
US10400272B1 (en) 2018-04-26 2019-09-03 Omniome, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
CN112243461A (zh) 2018-05-03 2021-01-19 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
EP4234717A3 (de) 2018-05-03 2023-11-01 Becton, Dickinson and Company Multiomics-probenanalyse mit hohem durchsatz
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
RU2766688C2 (ru) 2018-05-15 2022-03-15 Иллумина, Инк. Композиции и способы химического расщепления и снятия защиты для связанных с поверхностью олигонуклеотидов
AU2019275457B2 (en) 2018-05-25 2021-11-04 Illumina, Inc. Circulating RNA signatures specific to preeclampsia
CA3101337A1 (en) 2018-05-31 2019-12-05 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US20190385700A1 (en) 2018-06-04 2019-12-19 Guardant Health, Inc. METHODS AND SYSTEMS FOR DETERMINING The CELLULAR ORIGIN OF CELL-FREE NUCLEIC ACIDS
DK3810774T3 (da) 2018-06-04 2023-12-11 Illumina Inc Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
US20200251183A1 (en) 2018-07-11 2020-08-06 Illumina, Inc. Deep Learning-Based Framework for Identifying Sequence Patterns that Cause Sequence-Specific Errors (SSEs)
AU2019310041A1 (en) 2018-07-23 2021-02-04 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
CA3106035A1 (en) 2018-08-07 2020-02-13 The Broad Institute, Inc. Cas12b enzymes and systems
JP7431802B2 (ja) 2018-08-15 2024-02-15 イルミナ インコーポレイテッド ライブラリー富化を改善するための組成物および方法
EP3841202B1 (de) 2018-08-20 2023-10-04 Bio-Rad Laboratories, Inc. Generierung von nukleotidsequenzen durch barcode-kügelchen-co-lokalisierung in partitionen
CN109161536B (zh) * 2018-08-20 2022-04-08 天津科技大学 制备尿苷酸用酶制剂以及酶催化制备尿苷酸的方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
AU2019331907A1 (en) 2018-08-30 2021-04-08 Guardant Health, Inc. Methods and systems for detecting contamination between samples
CA3109539A1 (en) 2018-08-31 2020-03-05 Guardant Health, Inc. Microsatellite instability detection in cell-free dna
WO2020096691A2 (en) 2018-09-04 2020-05-14 Guardant Health, Inc. Methods and systems for detecting allelic imbalance in cell-free nucleic acid samples
GB2574746B (en) 2018-09-17 2020-06-03 Omniome Inc Engineered polymerases for improved sequencing
DK3814533T3 (da) 2018-09-20 2021-11-15 Tamirna Gmbh Mikro-RNA-signaturer til forudsigelse af leverdysfunktion
CN112805389A (zh) 2018-10-01 2021-05-14 贝克顿迪金森公司 确定5’转录物序列
US20210396756A1 (en) 2018-10-03 2021-12-23 The Broad Institute, Inc. Crispr effector system based diagnostics for hemorrhagic fever detection
AU2019272062B2 (en) 2018-10-15 2021-08-19 Illumina, Inc. Deep learning-based techniques for pre-training deep convolutional neural networks
WO2020086843A1 (en) 2018-10-26 2020-04-30 Illumina, Inc. Modulating polymer beads for dna processing
CN112673098B (zh) 2018-10-31 2023-01-06 亿明达股份有限公司 聚合酶、组合物及使用方法
EP3874060A1 (de) 2018-10-31 2021-09-08 Guardant Health, Inc. Verfahren, zusammensetzungen und systeme zur kalibrierung epigenetischer partitionstests
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
AU2019390388A1 (en) 2018-11-30 2021-05-06 Illumina, Inc. Analysis of multiple analytes using a single assay
GB2578528B (en) 2018-12-04 2021-02-24 Omniome Inc Mixed-phase fluids for nucleic acid sequencing and other analytical assays
AU2019391093A1 (en) 2018-12-05 2021-01-07 Illumina Cambridge Limited Polymerases, compositions, and methods of use
EP3891305A1 (de) 2018-12-05 2021-10-13 Illumina Cambridge Limited Verfahren und zusammensetzungen zur clustererzeugung durch brückenverstärkung
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
SG11202012748TA (en) 2018-12-14 2021-01-28 Illumina Cambridge Ltd Decreasing phasing with unlabeled nucleotides during sequencing
AU2019411267A1 (en) 2018-12-17 2021-01-07 Illumina Cambridge Limited Primer oligonucleotide for sequencing
WO2020126593A1 (en) 2018-12-17 2020-06-25 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
CA3103633A1 (en) 2018-12-18 2020-06-25 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
EP3899032A2 (de) 2018-12-20 2021-10-27 Omniome, Inc. Temperaturkontrolle zur analyse von nukleinsäuren und anderen analyten
CN113454218A (zh) 2018-12-20 2021-09-28 夸登特健康公司 用于改进核酸分子的回收的方法、组合物和系统
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN112930405A (zh) 2019-01-11 2021-06-08 Illumina剑桥有限公司 复合表面结合的转座体复合物
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (de) 2019-01-23 2023-04-05 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
WO2020154546A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2020160414A1 (en) 2019-01-31 2020-08-06 Guardant Health, Inc. Compositions and methods for isolating cell-free dna
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
WO2020172444A1 (en) 2019-02-20 2020-08-27 Omniome, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
JP2022522917A (ja) 2019-03-01 2022-04-21 イルミナ インコーポレイテッド ハイスループット単一核及び単一細胞ライブラリー、並びに製造及び使用方法
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
EP3976822A1 (de) 2019-05-31 2022-04-06 Guardant Health, Inc. Verfahren und systeme zur verbesserung der patientenüberwachung nach der operation
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
EP3997224A1 (de) 2019-07-12 2022-05-18 Illumina Cambridge Limited Herstellung einer nukleinsäurebibliothek unter verwendung von elektrophorese
CA3125241A1 (en) 2019-07-12 2021-01-21 Illumina Cambridge Limited Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
EP4004231A1 (de) 2019-07-22 2022-06-01 Becton, Dickinson and Company Test für einzelzell-chromatin-immunpräzipitationssequenzierung
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (de) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible nukleotide änderung
WO2021050962A1 (en) 2019-09-11 2021-03-18 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cancer detection and classification
CN114599795A (zh) 2019-10-18 2022-06-07 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
DK3812472T3 (da) 2019-10-21 2023-02-20 Univ Freiburg Albert Ludwigs Virkelig uvildig in vitro-undersøgelse til profilering af off-target-aktivitet af en eller flere målspecifikke programmerbare nukleaser i celler (abnoba-seq)
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
WO2021092431A1 (en) 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
EP4055185A1 (de) 2019-11-08 2022-09-14 10X Genomics, Inc. Räumlich markierte analytfänger für analyt-multiplexing
EP4010495A1 (de) 2019-11-22 2022-06-15 Illumina, Inc. Für präeklampsie spezifische zirkulierende rna-signaturen
CN114746560A (zh) 2019-11-26 2022-07-12 夸登特健康公司 改进甲基化多核苷酸结合的方法、组合物和系统
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CA3131632A1 (en) 2019-12-04 2021-06-10 Tong Liu Preparation of dna sequencing libraries for detection of dna pathogens in plasma
CN114008199A (zh) 2019-12-19 2022-02-01 伊路敏纳公司 高通量单细胞文库及其制备和使用方法
FI3891300T3 (fi) 2019-12-23 2023-05-10 10X Genomics Inc Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
CN115243792A (zh) 2020-02-04 2022-10-25 加利福尼亚太平洋生物科学股份有限公司 流通池及其制造和使用方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN111311265B (zh) * 2020-02-13 2023-07-25 布比(北京)网络技术有限公司 区块链私密交易证明方法、装置、计算机设备和存储介质
EP4107735A2 (de) 2020-02-20 2022-12-28 Illumina, Inc. Auf künstlicher intelligenz basierendes many-to-many-base-calling
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CA3176615A1 (en) 2020-03-30 2021-10-07 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
EP4242325A3 (de) 2020-04-22 2023-10-04 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
WO2021222828A1 (en) 2020-04-30 2021-11-04 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4146822A1 (de) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Zusammensetzungen und verfahren zur modifizierung von polymerase-nukleinsäurekomplexen
EP4150065A2 (de) 2020-05-12 2023-03-22 Illumina Inc Erzeugung von nukleinsäuren mit modifizierten basen unter verwendung rekombinanter terminaler deoxynucleotidyltransferase
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
JP2023526252A (ja) 2020-05-14 2023-06-21 ガーダント ヘルス, インコーポレイテッド 相同組換え修復欠損の検出
EP4153776A1 (de) 2020-05-22 2023-03-29 10X Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
EP4153775A1 (de) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
EP4231174A3 (de) 2020-06-11 2023-11-01 Nautilus Subsidiary, Inc. Verfahren und syteme zur rechnerischen decodierung biologischer, chemischer und physikalischer einheiten
CA3177293A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US20230242967A1 (en) 2020-07-02 2023-08-03 Illumina, Inc. A method to calibrate nucleic acid library seeding efficiency in flowcells
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
BR112023000073A2 (pt) 2020-07-08 2023-04-11 Illumina Inc Microesferas como transportadores de transpossomos
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4189111A1 (de) 2020-07-30 2023-06-07 Guardant Health, Inc. Verfahren zur isolierung von zellfreier dna
IL300112A (en) 2020-08-06 2023-03-01 Illumina Inc Preparation of RNA and DNA sequencing libraries using bead-linked transpososomes
WO2022040176A1 (en) 2020-08-18 2022-02-24 Illumina, Inc. Sequence-specific targeted transposition and selection and sorting of nucleic acids
EP4205126A1 (de) 2020-08-25 2023-07-05 Guardant Health, Inc. Verfahren und systeme zur vorhersage des ursprungs einer variante
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
CA3188197A1 (en) 2020-09-11 2022-03-17 Andrew Slatter Methods of enriching a target sequence from a sequencing library using hairpin adaptors
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US20220154285A1 (en) 2020-09-30 2022-05-19 Guardant Health, Inc. Analysis of methylated dna comprising methylation-sensitive or methylation-dependent restrictions
WO2022087150A2 (en) 2020-10-21 2022-04-28 Illumina, Inc. Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (de) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profilierung von stark exprimierten und schwach exprimierten proteinen
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4267757A1 (de) 2020-12-23 2023-11-01 Guardant Health, Inc. Verfahren und systeme zur analyse methylierter polynukleotide
CN116997411A (zh) 2021-01-13 2023-11-03 加利福尼亚太平洋生物科学股份有限公司 使用胶体组装的表面结构化
JP2024505508A (ja) 2021-01-29 2024-02-06 イルミナ インコーポレイテッド ポリヌクレオチドによるフローセルの播種効率を改善するための方法、組成物、及びキット
WO2022169972A1 (en) 2021-02-04 2022-08-11 Illumina, Inc. Long indexed-linked read generation on transposome bound beads
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
KR20230156364A (ko) 2021-03-05 2023-11-14 가던트 헬쓰, 인크. 분자 반응을 분석하기 위한 방법 및 관련 측면
US20220344004A1 (en) 2021-03-09 2022-10-27 Guardant Health, Inc. Detecting the presence of a tumor based on off-target polynucleotide sequencing data
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
JP2024511766A (ja) 2021-03-29 2024-03-15 イルミナ インコーポレイテッド 改善されたライブラリ調製方法
JP2024513187A (ja) 2021-03-29 2024-03-22 イルミナ インコーポレイテッド ライブラリー中のdna損傷を評価し、アンプリコンサイズバイアスを正規化するための組成物及び方法
AU2022246579A1 (en) 2021-03-30 2023-09-21 Illumina, Inc. Improved methods of isothermal complementary dna and library preparation
WO2022212180A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Artificial intelligence-based base caller with contextual awareness
EP4314283A1 (de) 2021-03-31 2024-02-07 Illumina, Inc. Verfahren zur herstellung von direktionalen tagmentationssequenzierungsbibliotheken mit transposonbasierter technologie mit eindeutigen molekularen identifikatoren zur fehlerkorrektur
CN117043867A (zh) 2021-04-02 2023-11-10 因美纳有限公司 用于检测用于测序的核苷酸样品玻片内的气泡的机器学习模型
US20220336054A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures
US20220356515A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
WO2022240764A1 (en) 2021-05-10 2022-11-17 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
EP4341434A1 (de) 2021-05-20 2024-03-27 Illumina, Inc. Zusammensetzungen und verfahren zur sequenzierung durch synthese
CN117677435A (zh) 2021-06-15 2024-03-08 伊鲁米纳公司 用于测序的不含水凝胶的表面官能化
US20220411864A1 (en) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
CN117546245A (zh) 2021-06-29 2024-02-09 因美纳有限公司 用于生成基因组坐标的置信度分类的机器学习模型
KR20240027599A (ko) 2021-06-29 2024-03-04 일루미나, 인코포레이티드 올리고 서열을 사용하여 훈련된 자체-학습 염기 호출자
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
KR20240022490A (ko) 2021-06-29 2024-02-20 일루미나, 인코포레이티드 뉴클레오티드 염기 호출 및 염기 호출 품질을 결정하기 위한 신호-대-잡음비 메트릭
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US20230027409A1 (en) 2021-07-13 2023-01-26 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
WO2023004357A1 (en) 2021-07-23 2023-01-26 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
KR20240037882A (ko) 2021-07-28 2024-03-22 일루미나, 인코포레이티드 염기 호출 시스템의 품질 점수 보정
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
US20230047225A1 (en) 2021-08-14 2023-02-16 Illumina, Inc. Polymerases, compositions, and methods of use
WO2023023500A1 (en) 2021-08-17 2023-02-23 Illumina, Inc. Methods and compositions for identifying methylated cytosines
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20230093253A1 (en) 2021-09-17 2023-03-23 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
WO2023049558A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. A graph reference genome and base-calling approach using imputed haplotypes
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
WO2023056328A2 (en) 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
US20230114905A1 (en) 2021-10-11 2023-04-13 Nautilus Biotechnology, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
US20230343415A1 (en) 2021-12-02 2023-10-26 Illumina Software, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023164660A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Calibration sequences for nucelotide sequencing
US20230313271A1 (en) 2022-02-25 2023-10-05 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
US20230314324A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
AU2023248405A1 (en) 2022-04-07 2024-01-04 Illumina, Inc. Altered cytidine deaminases and methods of use
US20230360732A1 (en) 2022-04-25 2023-11-09 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
US20230340571A1 (en) 2022-04-26 2023-10-26 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
US20230348967A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20230360725A1 (en) 2022-05-09 2023-11-09 Guardant Health, Inc. Detecting degradation based on strand bias
WO2023220627A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
WO2023235353A2 (en) 2022-06-03 2023-12-07 Illumina, Inc. Circulating rna biomarkers for preeclampsia
US20230407386A1 (en) 2022-06-09 2023-12-21 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2023240494A1 (zh) * 2022-06-15 2023-12-21 深圳华大智造科技股份有限公司 测序缓冲液及提高具有可逆阻断基团修饰的dNTP的稳定性的方法
WO2023250504A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
WO2024006779A1 (en) 2022-06-27 2024-01-04 Illumina, Inc. Accelerators for a genotype imputation model
WO2024006769A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Generating and implementing a structural variation graph genome
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
US20240038327A1 (en) 2022-07-26 2024-02-01 Illumina Software, Inc. Rapid single-cell multiomics processing using an executable file
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
US20240094215A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073516A1 (en) 2022-09-29 2024-04-04 Illumina, Inc. A target-variant-reference panel for imputing target variants
WO2024073519A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Machine-learning model for refining structural variant calls
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024068971A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides

Family Cites Families (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US6207421B1 (en) * 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5366603A (en) * 1984-03-29 1994-11-22 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes
US5230781A (en) * 1984-03-29 1993-07-27 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4997928A (en) * 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5232075A (en) * 1991-06-25 1993-08-03 New Venture Gear, Inc. Viscous coupling apparatus with coined plates
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
JP3227189B2 (ja) * 1991-12-25 2001-11-12 キヤノン株式会社 フレキシブルケーブルを備えた装置及び該装置を具備するインクジェット記録装置
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
CA2153387A1 (en) * 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US5677196A (en) * 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
WO1995006138A1 (en) * 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6593148B1 (en) * 1994-03-01 2003-07-15 Li-Cor, Inc. Cyanine dye compounds and labeling methods
US5601982A (en) * 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US5961923A (en) * 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5661028A (en) * 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
US6165765A (en) * 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
US5804386A (en) * 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
CA2281205A1 (en) * 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
EP2202312B1 (de) * 1997-03-12 2016-01-06 Applied Biosystems, LLC DNA-Polymerase mit verbesserten etikettierten Nucleotideinbindungseigenschaften
PT1017848E (pt) 1997-07-28 2003-02-28 Medical Biosystems Ltd Analise de sequencias de acido nucleico
EP1082458A1 (de) * 1998-05-01 2001-03-14 Arizona Board Of Regents Verfahren zur bestimmung der nukleotidsequenz von oligonukleotiden und dns molekülen
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6280939B1 (en) * 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
DE19844931C1 (de) * 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6221592B1 (en) * 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6044744A (en) * 1998-10-29 2000-04-04 At&T Corp. Fiber optic cable sheath removal tool
DE69930310T3 (de) * 1998-12-14 2009-12-17 Pacific Biosciences of California, Inc. (n. d. Ges. d. Staates Delaware), Menlo Park Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
TW461073B (en) 1998-12-15 2001-10-21 Matsushita Electric Ind Co Ltd Clock phase adjust method, and integrated circuit and design method of the same
US6558945B1 (en) * 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
EP1159453B1 (de) * 1999-03-10 2008-05-28 ASM Scientific, Inc. Methode zur direkten sequenzierung von nukleinsäuren
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
AU7086800A (en) * 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6399335B1 (en) * 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6917726B2 (en) * 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
US6869764B2 (en) * 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
CN101525660A (zh) * 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
ATE315066T1 (de) * 2000-09-19 2006-02-15 Li Cor Inc Cyaninfarbstoffe
WO2002044425A2 (en) * 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002087123A1 (en) * 2001-04-25 2002-10-31 Tekelec Methods and systems for load sharing signaling messages among signaling links
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US20030064400A1 (en) * 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7005518B2 (en) * 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
AU2003265857A1 (en) * 2003-02-05 2004-09-06 Amersham Biosciences Corp Nucleic acid amplification
EP1592779A4 (de) * 2003-02-05 2007-12-12 Ge Healthcare Bio Sciences Phosphat-endmarkierte nukleotide mit neuen linkern
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
KR100558528B1 (ko) * 2003-09-25 2006-03-10 동부아남반도체 주식회사 시모스 이미지 센서 및 그 제조방법
JP2008513782A (ja) * 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子解析のための装置及び方法
US7170050B2 (en) * 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2006086673A2 (en) * 2005-02-09 2006-08-17 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof
US7130041B2 (en) * 2005-03-02 2006-10-31 Li-Cor, Inc. On-chip spectral filtering using CCD array for imaging and spectroscopy
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
US7509836B2 (en) * 2005-09-01 2009-03-31 Li-Cor, Inc. Gas flux system chamber design and positioning method
WO2007064597A2 (en) * 2005-11-28 2007-06-07 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
US20070154921A1 (en) * 2005-12-16 2007-07-05 Applera Corporation Method and System for Phase-Locked Sequencing
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same

Also Published As

Publication number Publication date
US20070172864A1 (en) 2007-07-26
US20070292867A1 (en) 2007-12-20
US20090275036A1 (en) 2009-11-05
US20100317005A1 (en) 2010-12-16
US20050260614A1 (en) 2005-11-24
US20070172867A1 (en) 2007-07-26
US20070275395A1 (en) 2007-11-29
DE60131194T2 (de) 2008-08-07
EP1975251A3 (de) 2009-03-25
ATE377093T1 (de) 2007-11-15
US20070184475A1 (en) 2007-08-09
US20050266424A1 (en) 2005-12-01
EP1975251A2 (de) 2008-10-01
CN1553953A (zh) 2004-12-08
US7329492B2 (en) 2008-02-12
US20070172863A1 (en) 2007-07-26
US20070172862A1 (en) 2007-07-26
CN101525660A (zh) 2009-09-09
EP1368460A2 (de) 2003-12-10
US20110014604A1 (en) 2011-01-20
EP1368460B1 (de) 2007-10-31
JP2009118847A (ja) 2009-06-04
EP2100971A3 (de) 2009-11-25
US20110021383A1 (en) 2011-01-27
AU8288101A (en) 2002-01-21
WO2002004680A2 (en) 2002-01-17
US20100304367A1 (en) 2010-12-02
JP2009240318A (ja) 2009-10-22
JP2004513619A (ja) 2004-05-13
AU2001282881B2 (en) 2007-06-14
CA2415897A1 (en) 2002-01-17
US20070172865A1 (en) 2007-07-26
US20070172868A1 (en) 2007-07-26
US20030064366A1 (en) 2003-04-03
US20100255463A1 (en) 2010-10-07
EP2100971A2 (de) 2009-09-16
US20070172861A1 (en) 2007-07-26
WO2002004680A3 (en) 2003-10-16
US20070172858A1 (en) 2007-07-26
CN100462433C (zh) 2009-02-18
US20110059436A1 (en) 2011-03-10
US20090305278A1 (en) 2009-12-10

Similar Documents

Publication Publication Date Title
DE60131194D1 (de) Sequenzbestimmung in echtzeit
ATE426814T1 (de) Markierungsmittel mit einer vielzahl von farbtínen
BRPI0517712A (pt) estrutura de segurança destinada a ser incorporada ou fixada pelo menos parcialmente em ou sobre um artigo, artigo e processos de fabricação e de autenticação e/ou de identificação de um artigo
ATE439452T1 (de) Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen
DK0699768T3 (da) Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober
DE69735313D1 (de) Fluoreszenz-Donor-Akzeptor Paar
ATE414170T1 (de) Wellenlängen verschiebende proben und primer
DE60121177D1 (de) Farbstoffverbindungen mit copolymerisierbaren vinylgruppen
WO2009033021A3 (en) Light source with wavelength converting phosphor
DK1163103T3 (da) Anvendelse af gasplasmahydrofiliseret overflade som en væskekontaktflade og microfabrikeret anordning indeholdende en gasplasmahudrofiliseret overflade
ATE286060T1 (de) Präpolymere (meth)acrylate mit polycyclischen oder aromatischen segmenten
DE50210457D1 (de) Vorrichtung zur referenzierung von fluoreszenzsignalen
DE60316569D1 (de) Beleuchtungsvorrichtung zur simulation von neonlicht unter verwending von fluoreszierenden farbstoffen
EP1043382A3 (de) Polymere fluoreszierende Substanz und lichtemittierende Vorrichtung auf Polymerbasis
CA2342767A1 (en) Multihued labels
ID30599A (id) Metode pengevaluasian putihnya, metode pengevaluasian pembanding putihnya, sumber cahaya dan luminaire
FR2873128B1 (fr) Procede de marquage d'un materiau et materiau ainsi marque
ATE488527T1 (de) Fluoreszenzprotein
DE60023954D1 (de) Naphthyloxyalkyl(meth)acrylate mit hohem brechungsindex und niedriger glasübergangstemperatur
DE112004001215D2 (de) Handlampe, insbesondere für die magnetische Rissprüfung
DE60114804D1 (de) Konstruierte fluoreszenzproteine mit langen wellenlängen
ATE554426T1 (de) Verfahren zur ermöglichung der übertragung von im wesentlichen gleichen energiemengen
ATE348129T1 (de) Ethylenisch ungesättigte blaue antrachinonfarbstoffe
DE502004009281D1 (de) Mikroskop mit evaneszenter probenbeleuchtung
DE602006015227D1 (de) Homogener lumineszenz-bioassay

Legal Events

Date Code Title Description
8363 Opposition against the patent
8327 Change in the person/name/address of the patent owner

Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US

8328 Change in the person/name/address of the agent

Representative=s name: DERZEIT KEIN VERTRETER BESTELLT

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN