DE60141087D1 - Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren - Google Patents
Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäurenInfo
- Publication number
- DE60141087D1 DE60141087D1 DE60141087T DE60141087T DE60141087D1 DE 60141087 D1 DE60141087 D1 DE 60141087D1 DE 60141087 T DE60141087 T DE 60141087T DE 60141087 T DE60141087 T DE 60141087T DE 60141087 D1 DE60141087 D1 DE 60141087D1
- Authority
- DE
- Germany
- Prior art keywords
- amplification
- methods
- transcription
- module
- rna transcripts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21390800P | 2000-06-26 | 2000-06-26 | |
US27774801P | 2001-03-21 | 2001-03-21 | |
PCT/US2001/020660 WO2002000938A2 (en) | 2000-06-26 | 2001-06-26 | Methods and compositions for transcription-based nucleic acid amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60141087D1 true DE60141087D1 (de) | 2010-03-04 |
Family
ID=26908503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60141087T Expired - Lifetime DE60141087D1 (de) | 2000-06-26 | 2001-06-26 | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
Country Status (10)
Country | Link |
---|---|
US (3) | US6686156B2 (de) |
EP (1) | EP1356094B1 (de) |
JP (1) | JP2004513617A (de) |
CN (1) | CN1498274A (de) |
AT (1) | ATE455186T1 (de) |
AU (1) | AU2001271595A1 (de) |
CA (1) | CA2412721A1 (de) |
DE (1) | DE60141087D1 (de) |
MX (1) | MXPA02012735A (de) |
WO (1) | WO2002000938A2 (de) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
WO2002000938A2 (en) * | 2000-06-26 | 2002-01-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
WO2002048402A2 (en) * | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
JP3681729B2 (ja) | 2001-02-15 | 2005-08-10 | タカラバイオ株式会社 | 塩基置換の検出方法 |
US7094536B2 (en) * | 2001-03-09 | 2006-08-22 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
ZA200210369B (en) * | 2001-03-09 | 2004-07-08 | Nugen Technologies Inc | Methods and compositions for amplification or RNA sequences. |
US8137911B2 (en) * | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
US7176025B2 (en) * | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
US20030219729A1 (en) * | 2002-03-15 | 2003-11-27 | Tosoh Corporation | Unary avian myeloblastosis virus revers transcriptase and its use |
US20040005614A1 (en) * | 2002-05-17 | 2004-01-08 | Nurith Kurn | Methods for fragmentation, labeling and immobilization of nucleic acids |
JP3923917B2 (ja) * | 2003-03-26 | 2007-06-06 | 株式会社東芝 | ターゲットの製造方法、標的配列検出方法、ターゲットおよび標的配列検出用アッセイキット |
WO2004092418A2 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
EP1711591A4 (de) | 2003-12-29 | 2010-04-28 | Nugen Technologies Inc | Verfahren zur analyse des methylierungsstatus von nukleinsäure und verfahren zur fragmentierung, markierung und immobilisierung von nukleinsäuren |
US7713697B2 (en) * | 2004-08-27 | 2010-05-11 | Gen-Probe Incorporated | Methods and kits for amplifying DNA |
EP2071031B1 (de) * | 2004-08-27 | 2013-10-09 | Gen-Probe Incorporated | Einfach-Primernukleinsäuren-Erweiterungsverfahren |
KR100695134B1 (ko) * | 2004-11-25 | 2007-03-14 | 삼성전자주식회사 | 층류를 이용한 마이크로어레이 및 이의 제조 방법 |
EP1836302B1 (de) * | 2004-12-23 | 2010-04-14 | Amersham Biosciences Corp. | Rna-amplifikation auf ligationsbasis |
EP1841879A4 (de) | 2005-01-25 | 2009-05-27 | Population Genetics Technologi | Isothermische dna-amplifikation |
US20070020639A1 (en) * | 2005-07-20 | 2007-01-25 | Affymetrix, Inc. | Isothermal locus specific amplification |
CA2621267A1 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
WO2007087262A2 (en) * | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Selective genome amplification |
WO2007120843A2 (en) * | 2006-04-12 | 2007-10-25 | Siemens Healthcare Diagnostics Inc. | Single nucleotide polymorphism detection from unamplified genomic dna |
CA2652052A1 (en) * | 2006-05-16 | 2007-11-29 | Nugen Technologies, Inc. | Nucleic acid separation and purification method based on reversible charge interactions |
US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US7501254B2 (en) * | 2006-07-20 | 2009-03-10 | Ghc Technologies, Inc. | Methods and compositions for amplification and capture of nucleic acid sequences |
JP5340167B2 (ja) * | 2006-12-21 | 2013-11-13 | ジェン−プロウブ インコーポレイテッド | 核酸増幅のための方法および組成物 |
US8183359B2 (en) * | 2007-03-01 | 2012-05-22 | Gen-Probe Incorporated | Kits for amplifying DNA |
US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
US9388457B2 (en) | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
PL2215265T3 (pl) | 2007-11-14 | 2014-04-30 | Grifols Therapeutics Inc | Sposób opracowania wyników urządzenia do łańcuchowej reakcji polimeryzacji (PCR) |
US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
WO2010126913A1 (en) | 2009-04-27 | 2010-11-04 | Gen-Probe Incorporated | Methods and kits for use in the selective amplification of target sequences |
US9169512B2 (en) | 2009-07-01 | 2015-10-27 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
CA2773887A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
WO2011053987A1 (en) * | 2009-11-02 | 2011-05-05 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence selection and amplification |
WO2011091393A1 (en) * | 2010-01-25 | 2011-07-28 | Rd Biosciences, Inc. | Self-folding amplification of target nucleic acid |
EP2769007B1 (de) | 2011-10-19 | 2016-12-07 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren |
EP2798089B1 (de) | 2011-12-30 | 2018-05-23 | Bio-rad Laboratories, Inc. | Verfahren und zusammensetzungen zur durchführung von nucleinsäureamplifikationsreaktionen |
WO2013112923A1 (en) | 2012-01-26 | 2013-08-01 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
JP6445426B2 (ja) | 2012-05-10 | 2018-12-26 | ザ ジェネラル ホスピタル コーポレイション | ヌクレオチド配列を決定する方法 |
CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
US9777319B2 (en) | 2012-06-29 | 2017-10-03 | General Electric Company | Method for isothermal DNA amplification starting from an RNA template |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
ES2761920T3 (es) * | 2012-08-30 | 2020-05-21 | Gen Probe Inc | Amplificación de ácido nucleico multifásica |
MY167603A (en) | 2012-10-04 | 2018-09-20 | The Board Of Trustees Of Leland Stanford Junior Univ | Method and reagents for detection, quantitation, and serotyping of dengue viruses |
JP6338221B2 (ja) | 2012-10-24 | 2018-06-06 | タカラ バイオ ユーエスエー, インコーポレイテッド | 核酸生成物を生成するための、テンプレートスイッチに基づく方法 |
WO2014144092A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
WO2015057319A1 (en) | 2013-10-17 | 2015-04-23 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
EP3068883B1 (de) | 2013-11-13 | 2020-04-29 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung |
WO2015094861A1 (en) | 2013-12-17 | 2015-06-25 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
WO2015112974A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
SG11201700891SA (en) | 2014-08-06 | 2017-03-30 | Nugen Technologies Inc | Digital measurements from targeted sequencing |
CA3037190A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
AU2017328950B2 (en) | 2016-09-15 | 2023-09-14 | Archerdx, Llc | Methods of nucleic acid sample preparation |
PL3551753T3 (pl) * | 2016-12-09 | 2022-10-31 | The Broad Institute, Inc. | Diagnostyka oparta na układzie efektorowym crispr |
CN106529206B (zh) * | 2016-12-20 | 2019-02-22 | 大连海事大学 | 一种蛋白质二维结构图功能元件的自动布线方法 |
US20200181720A1 (en) * | 2017-03-15 | 2020-06-11 | The Broad Institute, Inc. | Crispr effector system based diagnostics for virus detection |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
CN113528624A (zh) * | 2020-04-17 | 2021-10-22 | 青岛大学 | 扩增和检测核酸的方法及试剂盒 |
Family Cites Families (107)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR8108820A (pt) | 1980-09-24 | 1982-08-24 | Cetus Corp | Processo e sonda de diagnostico |
EP0084796B1 (de) | 1982-01-22 | 1990-05-02 | Cetus Corporation | Verfahren zur Charakterisierung von HLA und die darin benutzten cDNS-Testmittel |
US4582788A (en) | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
US4786600A (en) | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
DE3634356A1 (de) | 1986-10-08 | 1988-04-21 | Epis Sa | Arzneimittel enthaltend alpha-halogenierte dicarbonsaeuren |
IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
US6090591A (en) | 1987-07-31 | 2000-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
US6004745A (en) | 1987-09-21 | 1999-12-21 | Gen-Probe Incorporated | Hybridization protection assay |
US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
AU622426B2 (en) | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
EP0365627B1 (de) | 1988-03-24 | 1993-12-22 | University Of Iowa Research Foundation | Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
AU650622B2 (en) | 1989-07-11 | 1994-06-30 | Gen-Probe Incorporated | Nucleic acid sequence amplification methods utilizing a transcription complex |
US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
US5427911A (en) | 1990-05-01 | 1995-06-27 | Yale University | Coupled amplification and sequencing of DNA |
HU218095B (hu) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
US5846710A (en) | 1990-11-02 | 1998-12-08 | St. Louis University | Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension |
AU8997991A (en) | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5888819A (en) | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
JP2843675B2 (ja) | 1992-03-11 | 1999-01-06 | ダナ−ファーバー・キャンサー・インスチチュート・インコーポレーテッド | メッセンジャーrnaの同定、単離およびクローニング |
CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
US5710028A (en) | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
ATE152180T1 (de) | 1992-07-31 | 1997-05-15 | Behringwerke Ag | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
ZA936016B (en) | 1992-08-24 | 1994-03-10 | Akzo Nv | Method for nucleic acid amplification |
US5591575A (en) | 1993-04-07 | 1997-01-07 | Amersham International Plc | Subtraction hybridization employing aziridinylbenoquinone cross-linking agents |
US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
JPH0723799A (ja) | 1993-07-13 | 1995-01-27 | Hitachi Ltd | ポリヌクレオチドの検出方法 |
US6027923A (en) | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
AU7487294A (en) | 1993-08-18 | 1995-03-14 | Id Biomedical Corporation | Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules |
AU8102694A (en) | 1993-11-17 | 1995-06-06 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
US5556752A (en) | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
US5665545A (en) | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5763178A (en) | 1995-06-07 | 1998-06-09 | Trevigen, Inc. | Oscillating signal amplifier for nucleic acid detection |
US5882867A (en) | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
FR2737223B1 (fr) | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
US5871697A (en) | 1995-10-24 | 1999-02-16 | Curagen Corporation | Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
DE69612013T2 (de) | 1995-11-21 | 2001-08-02 | Univ Yale New Haven | Unimolekulare segmentamplifikation und bestimmung |
JP4073038B2 (ja) | 1995-12-15 | 2008-04-09 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | テルモアナエロバクター・テルモヒドロスルフリカスからの熱安定性dnaポリメラーゼおよびエキソヌクレアーゼ活性が除去されているその変異体酵素 |
US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
GB9604267D0 (en) | 1996-02-29 | 1996-05-01 | Royal Infirmary Of Edinburgh N | Mutation assay |
EP0912761A4 (de) | 1996-05-29 | 2004-06-09 | Cornell Res Foundation Inc | Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion |
US6218105B1 (en) * | 1996-07-19 | 2001-04-17 | Kathleen S. Hall | High throughput papilloma virus in vitro infectivity assay |
EP0942917B1 (de) | 1996-08-14 | 2015-02-25 | Life Technologies Corporation | Verwendung von stabile zubereitungen in nukleinsäureamplifikation und sequenzierung verfahren |
DE19653439A1 (de) | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
JP3666604B2 (ja) * | 1997-04-16 | 2005-06-29 | アプレラ コーポレーション | 核酸アーカイビング |
US6140086A (en) * | 1997-08-15 | 2000-10-31 | Fox; Donna K. | Methods and compositions for cloning nucleic acid molecules |
JP4317953B2 (ja) * | 1998-01-22 | 2009-08-19 | 独立行政法人理化学研究所 | Dnaの塩基配列決定方法 |
US20010000077A1 (en) | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
WO2000070095A2 (en) * | 1999-05-17 | 2000-11-23 | Dade Behring Inc. | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide |
US6132997A (en) | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
CA2384838C (en) * | 1999-09-13 | 2006-07-18 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6300073B1 (en) * | 1999-10-01 | 2001-10-09 | Clontech Laboratories, Inc. | One step RT-PCR methods, enzyme mixes and kits for use in practicing the same |
WO2001073134A2 (en) * | 2000-03-28 | 2001-10-04 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health | Gene profiling arrays |
WO2002000938A2 (en) | 2000-06-26 | 2002-01-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US6815164B2 (en) | 2000-10-06 | 2004-11-09 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
US6673549B1 (en) * | 2000-10-12 | 2004-01-06 | Incyte Corporation | Genes expressed in C3A liver cell cultures treated with steroids |
AR031640A1 (es) * | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
US7094536B2 (en) | 2001-03-09 | 2006-08-22 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
ZA200210369B (en) | 2001-03-09 | 2004-07-08 | Nugen Technologies Inc | Methods and compositions for amplification or RNA sequences. |
AU2002325538B2 (en) * | 2001-08-20 | 2007-03-22 | Takara Bio Inc. | Nucleic acid amplification methods |
US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
DE60232013D1 (de) * | 2001-11-20 | 2009-05-28 | Exact Sciences Corp | Automatische probenvorbereitungsverfahren und -vorrichtungen |
AU2003220249A1 (en) * | 2002-03-15 | 2003-09-29 | Arcturus Bioscience, Inc. | Improved nucleic acid amplification |
AU2004254552B2 (en) * | 2003-01-29 | 2008-04-24 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
WO2004092330A2 (en) * | 2003-04-11 | 2004-10-28 | Applera Corporation | Method of generating long nucleic acid molecules of defined sequence |
EP1711591A4 (de) * | 2003-12-29 | 2010-04-28 | Nugen Technologies Inc | Verfahren zur analyse des methylierungsstatus von nukleinsäure und verfahren zur fragmentierung, markierung und immobilisierung von nukleinsäuren |
JP2009506788A (ja) * | 2005-09-06 | 2009-02-19 | ジェン−プローブ・インコーポレーテッド | 核酸の等温増幅のための方法、組成物及びキット |
CA2652052A1 (en) * | 2006-05-16 | 2007-11-29 | Nugen Technologies, Inc. | Nucleic acid separation and purification method based on reversible charge interactions |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
-
2001
- 2001-06-26 WO PCT/US2001/020660 patent/WO2002000938A2/en active Application Filing
- 2001-06-26 US US09/893,191 patent/US6686156B2/en not_active Expired - Fee Related
- 2001-06-26 AT AT01950624T patent/ATE455186T1/de not_active IP Right Cessation
- 2001-06-26 EP EP01950624A patent/EP1356094B1/de not_active Expired - Lifetime
- 2001-06-26 JP JP2002506251A patent/JP2004513617A/ja not_active Withdrawn
- 2001-06-26 AU AU2001271595A patent/AU2001271595A1/en not_active Abandoned
- 2001-06-26 DE DE60141087T patent/DE60141087D1/de not_active Expired - Lifetime
- 2001-06-26 MX MXPA02012735A patent/MXPA02012735A/es not_active Application Discontinuation
- 2001-06-26 CA CA002412721A patent/CA2412721A1/en not_active Abandoned
- 2001-06-26 CN CNA018132979A patent/CN1498274A/zh active Pending
-
2003
- 2003-10-14 US US10/686,466 patent/US7294461B2/en not_active Expired - Fee Related
-
2007
- 2007-10-31 US US11/933,332 patent/US20080182300A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2002000938A3 (en) | 2003-08-28 |
US7294461B2 (en) | 2007-11-13 |
CA2412721A1 (en) | 2002-01-03 |
JP2004513617A (ja) | 2004-05-13 |
US6686156B2 (en) | 2004-02-03 |
WO2002000938A9 (en) | 2003-07-03 |
US20020058270A1 (en) | 2002-05-16 |
MXPA02012735A (es) | 2004-04-20 |
US20040203019A1 (en) | 2004-10-14 |
WO2002000938A2 (en) | 2002-01-03 |
AU2001271595A1 (en) | 2002-01-08 |
EP1356094A2 (de) | 2003-10-29 |
CN1498274A (zh) | 2004-05-19 |
ATE455186T1 (de) | 2010-01-15 |
EP1356094B1 (de) | 2010-01-13 |
US20080182300A1 (en) | 2008-07-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE60141087D1 (de) | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren | |
NO20021223D0 (no) | Fremgangsmåter og sammensetninger for line¶r isoterm amplifisering av polynukleotidsekvenser | |
DE60335637D1 (de) | Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren | |
ATE475720T1 (de) | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben | |
DE69928263D1 (de) | Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen | |
BR9808368A (pt) | Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. | |
ATE340857T1 (de) | Verfahren zur herstellung von l-aminosäuren durch erhöhung des zellulären nadph | |
WO2006102309A3 (en) | Methods, compositions, and kits for detection of micro rna | |
ATE361996T1 (de) | Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen | |
EA201891629A1 (ru) | Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов | |
PE20020259A1 (es) | Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas | |
ATE317024T1 (de) | Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden | |
DE602006014009D1 (de) | Nukleinsäure konstrukten, pharmazeutische kompositionen und methoden für die behandlung von krebs | |
DE602005020691D1 (de) | Rna-amplifikation auf ligationsbasis | |
ATE506436T1 (de) | Auf trans-splicing basierende amplifikationsvectoren | |
CL2009001601A1 (es) | Secuencia reguladora aisladas capaces de iniciar y conducir la transcripcion de un polinucleotido heterólogo en plantas, construccion génica que comprende dichos acidos nucleicos y metodo para producir una planta con un fenotipo modificado. (divisional solicitud n° 1425-05) | |
DE602008003757D1 (de) | Auswahl und anreicherung von proteinen durch in-vitro-untergliederung | |
HUP0303517A2 (hu) | Hímfertilitást közvetítő nukleotidszekvenciák és alkalmazásuk | |
TW200506067A (en) | Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence | |
MX2021005028A (es) | Ensamble determinista multiplexado de genotecas de adn. | |
RU2008149510A (ru) | Набор праймеров для использования при детекции дрожжей рода dekkera и дрожжей рода brettanomyces | |
AR014528A1 (es) | Adn que comprende una secuencia de nucleotidos que se puede traducir a una proteina con actividad de invertasa, proteina derivada del adn que tieneactividad de invertasa pero que carece de actividad beta-fructofuranosidasa; metodo para producir adn y dicho metodo donde el adn es producido por una | |
DE50313095D1 (de) | Sputterverfahren zur herstellung von eigenspannungsoptimierten beschichtungen | |
KR101881977B1 (ko) | 사계성 또는 일계성 딸기 품종 선별용 snp 마커 및 이의 용도 | |
MX2022006748A (es) | Composiciones de acidos nucleicos. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |