DE60314902D1 - Fehlpaarungsendonukleasen und verwendungsverfahren - Google Patents

Fehlpaarungsendonukleasen und verwendungsverfahren

Info

Publication number
DE60314902D1
DE60314902D1 DE60314902T DE60314902T DE60314902D1 DE 60314902 D1 DE60314902 D1 DE 60314902D1 DE 60314902 T DE60314902 T DE 60314902T DE 60314902 T DE60314902 T DE 60314902T DE 60314902 D1 DE60314902 D1 DE 60314902D1
Authority
DE
Germany
Prior art keywords
heteroduplex
complementarity
polynucleotide
mismatches
increased
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60314902T
Other languages
English (en)
Other versions
DE60314902T2 (de
Inventor
Hal S Padgett
Andrew A Vaewhongs
Fakhrieh S Vojdani
Mark L Smith
John A Lindbo
Wayne P Fitzmaurice
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Large Scale Biology Corp
Original Assignee
Large Scale Biology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/098,155 external-priority patent/US20030157495A1/en
Application filed by Large Scale Biology Corp filed Critical Large Scale Biology Corp
Publication of DE60314902D1 publication Critical patent/DE60314902D1/de
Application granted granted Critical
Publication of DE60314902T2 publication Critical patent/DE60314902T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
DE60314902T 2002-02-01 2003-01-31 Fehlpaarungsendonukleasen und verwendungsverfahren Expired - Lifetime DE60314902T2 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US98155 1987-09-18
US211079 1998-12-14
US35372202P 2002-02-01 2002-02-01
US353722P 2002-02-01
US10/098,155 US20030157495A1 (en) 2002-02-01 2002-03-14 Nucleic acid molecules encoding CEL I endonuclease and methods of use thereof
US10/211,079 US7078211B2 (en) 2002-02-01 2002-08-01 Nucleic acid molecules encoding endonucleases and methods of use thereof
PCT/US2003/002958 WO2003066809A2 (en) 2002-02-01 2003-01-31 Mismatch endonucleases and methods of use

Publications (2)

Publication Number Publication Date
DE60314902D1 true DE60314902D1 (de) 2007-08-23
DE60314902T2 DE60314902T2 (de) 2008-04-10

Family

ID=27739010

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60314902T Expired - Lifetime DE60314902T2 (de) 2002-02-01 2003-01-31 Fehlpaarungsendonukleasen und verwendungsverfahren

Country Status (8)

Country Link
US (4) US7078211B2 (de)
EP (1) EP1565557B1 (de)
JP (1) JP4395632B2 (de)
AT (1) ATE366807T1 (de)
AU (1) AU2003207776B2 (de)
CA (1) CA2473187C (de)
DE (1) DE60314902T2 (de)
WO (1) WO2003066809A2 (de)

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US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
EP1574570A1 (de) * 2004-03-12 2005-09-14 Universität Regensburg Verfahren zur Verringerung der Anzahl von fehlgepaarten Nukleotiden in doppelsträngigen Polynukleotiden
ES2292270B1 (es) * 2004-04-14 2009-02-16 Oryzon Genomics, S.A. Procedimiento para detectar selectivamente acidos nucleicos con estructuras atipicas convertibles en muescas.
US20090053261A1 (en) * 2005-09-08 2009-02-26 Lindbo John A Modified tobacco mosaic virus particles as scaffolds for display of protein antigens for vaccine applications
US20060029612A1 (en) * 2004-04-15 2006-02-09 Large Scale Biology Corporation Prevention and treatment of recurrent respiratory papillomatosis
US7729761B2 (en) * 2004-07-14 2010-06-01 Cardiac Pacemakers, Inc. Method and apparatus for controlled gene or protein delivery
CN102604914A (zh) * 2004-07-30 2012-07-25 法国植物基因组研究计划-华莱 高敏感的内切核酸酶的生产方法、内切核酸酶的新制品及其用途
BRPI0616012A2 (pt) * 2005-07-18 2011-05-31 Protalix Ltd administração via mucosa ou intestino de macromoléculas biologicamente ativas
WO2007101028A2 (en) * 2006-02-24 2007-09-07 Transgenomic, Inc. Method to produce enzymatically active recombinant endonucleases
US7888475B2 (en) * 2008-09-15 2011-02-15 Padgett Hal S Interferon alpha and interferon kappa fusion protein
WO2011102802A1 (en) * 2010-02-18 2011-08-25 Agency For Science, Technology And Research Method for reducing mismatches in double-stranded dna molecules
CN103945931B (zh) 2011-09-26 2017-03-22 基因技术股份公司 高效的小体积核酸合成
CA3156904A1 (en) * 2012-02-01 2013-08-08 Synthetic Genomics, Inc. Materials and methods for the synthesis of error-minimized nucleic acid molecules
US20130274129A1 (en) 2012-04-04 2013-10-17 Geneart Ag Tal-effector assembly platform, customized services, kits and assays
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20150119260A1 (en) * 2013-10-18 2015-04-30 National Taiwan University Circulating cancer biomarker and its use
EP3169781B1 (de) 2014-07-15 2020-04-08 Life Technologies Corporation Zusammensetzungen und verfahren zur nukleinsäureassemblierung
LT3557262T (lt) 2014-12-09 2022-11-10 Life Technologies Corporation Didelio efektyvumo nukleorūgščių sintezė mažame tūryje
WO2017007925A1 (en) 2015-07-07 2017-01-12 Thermo Fisher Scientific Geneart Gmbh Enzymatic synthesis of nucleic acid sequences
EP3763818A1 (de) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Vorrichtungen und verfahren zur herstellung von proteinen
JP2019502399A (ja) * 2016-01-22 2019-01-31 ビオーム バイオサイエンシズ プライベート リミティド アクネ菌を検出し治療する方法及びそのキット
WO2020001783A1 (en) 2018-06-29 2020-01-02 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
CN115244189A (zh) 2020-03-06 2022-10-25 生命科技股份有限公司 高序列保真度核酸合成及组装
US11667968B2 (en) 2021-05-27 2023-06-06 New England Biolabs, Inc. Fragmentation of DNA

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Also Published As

Publication number Publication date
ATE366807T1 (de) 2007-08-15
US7056740B2 (en) 2006-06-06
US20030148315A1 (en) 2003-08-07
US7273739B2 (en) 2007-09-25
WO2003066809A3 (en) 2005-06-30
US20030157682A1 (en) 2003-08-21
JP4395632B2 (ja) 2010-01-13
US7078211B2 (en) 2006-07-18
US20080145913A1 (en) 2008-06-19
DE60314902T2 (de) 2008-04-10
AU2003207776B2 (en) 2008-12-11
WO2003066809A2 (en) 2003-08-14
CA2473187C (en) 2012-07-31
EP1565557B1 (de) 2007-07-11
EP1565557A2 (de) 2005-08-24
US20060194288A1 (en) 2006-08-31
AU2003207776A1 (en) 2003-09-02
JP2005529586A (ja) 2005-10-06
EP1565557A4 (de) 2005-11-30
CA2473187A1 (en) 2003-08-14

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