DE60327717D1 - Zufuhr transposonbasierter vektoren zu fortpflanzungsorganen - Google Patents

Zufuhr transposonbasierter vektoren zu fortpflanzungsorganen

Info

Publication number
DE60327717D1
DE60327717D1 DE60327717T DE60327717T DE60327717D1 DE 60327717 D1 DE60327717 D1 DE 60327717D1 DE 60327717 T DE60327717 T DE 60327717T DE 60327717 T DE60327717 T DE 60327717T DE 60327717 D1 DE60327717 D1 DE 60327717D1
Authority
DE
Germany
Prior art keywords
based vectors
transposon
transgenic
supply
reproductive organs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60327717T
Other languages
English (en)
Inventor
Richard K Cooper
William C Fioretti
Gary G Cadd
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Louisiana State University and Agricultural and Mechanical College
TransGenRx Inc
Original Assignee
Louisiana State University and Agricultural and Mechanical College
TransGenRx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Louisiana State University and Agricultural and Mechanical College, TransGenRx Inc filed Critical Louisiana State University and Agricultural and Mechanical College
Application granted granted Critical
Publication of DE60327717D1 publication Critical patent/DE60327717D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L15/00—Egg products; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/02—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from eggs
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/23—Immunoglobulins specific features characterized by taxonomic origin from birds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/90—Vector systems having a special element relevant for transcription from vertebrates avian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
DE60327717T 2003-01-21 2003-12-24 Zufuhr transposonbasierter vektoren zu fortpflanzungsorganen Expired - Fee Related DE60327717D1 (de)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US44140503P 2003-01-21 2003-01-21
US44139203P 2003-01-21 2003-01-21
US44150203P 2003-01-21 2003-01-21
US44137703P 2003-01-21 2003-01-21
US44144703P 2003-01-21 2003-01-21
US44138103P 2003-01-21 2003-01-21
US10/609,019 US7527966B2 (en) 2002-06-26 2003-06-26 Gene regulation in transgenic animals using a transposon-based vector
PCT/US2003/041335 WO2004067707A2 (en) 2003-01-21 2003-12-24 Administration of transposon-based vectors to reproductive organs

Publications (1)

Publication Number Publication Date
DE60327717D1 true DE60327717D1 (de) 2009-07-02

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DE60327717T Expired - Fee Related DE60327717D1 (de) 2003-01-21 2003-12-24 Zufuhr transposonbasierter vektoren zu fortpflanzungsorganen

Country Status (7)

Country Link
US (2) US7527966B2 (de)
EP (2) EP1592789B1 (de)
JP (2) JP2006512922A (de)
AT (1) ATE431849T1 (de)
AU (3) AU2003303830A1 (de)
DE (1) DE60327717D1 (de)
WO (3) WO2004067743A1 (de)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
JPWO2005115133A1 (ja) * 2004-05-27 2008-03-27 国立大学法人京都大学 生体内精子形成細胞への遺伝子導入方法
EP1885856B1 (de) * 2005-05-17 2013-07-17 Temasek Life Sciences Laboratory Limited Transposition von mais-ac/ds-elementen in vertebraten
KR100830826B1 (ko) 2007-01-24 2008-05-19 씨제이제일제당 (주) 코리네박테리아를 이용하여 글리세롤을 포함한탄소원으로부터 발효산물을 생산하는 방법
WO2008091311A1 (en) * 2007-01-26 2008-07-31 Synageva Biopharma Corp Transgene expression in avians
EP2527445A3 (de) * 2007-05-16 2013-02-20 MAT Malta Advanced Technologies Limited Behandlung und Prävention von Grippe
KR101126041B1 (ko) 2008-04-10 2012-03-19 씨제이제일제당 (주) 트랜스포존을 이용한 형질전환용 벡터, 상기 벡터로형질전환된 미생물 및 이를 이용한 ｌ-라이신 생산방법
US8932861B2 (en) 2008-04-10 2015-01-13 Cj Cheiljedang Corporation Transformation vector comprising transposon, microorganisms transformed with the vector, and method for producing L-lysine using the microorganism
US8309791B2 (en) 2008-07-16 2012-11-13 Recombinectics, Inc. Method for producing a transgenic pig using a hyper-methylated transposon
US9157097B2 (en) * 2008-09-25 2015-10-13 Proteovec Holding, L.L.C. Vectors for production of growth hormone
EP2342224A2 (de) * 2008-09-25 2011-07-13 TransGenRx, Inc. Neue vektoren zur herstellung von interferon
US9150880B2 (en) * 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
WO2010118360A1 (en) * 2009-04-09 2010-10-14 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Production of proteins using transposon-based vectors
WO2011002988A1 (en) 2009-07-01 2011-01-06 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for severe combined immunodeficiency (scid)
WO2011017315A2 (en) * 2009-08-03 2011-02-10 Recombinetics, Inc. Methods and compositions for targeted gene modification
WO2012051615A1 (en) 2010-10-15 2012-04-19 Transgenrx, Inc. Novel vectors for production of glycosylated interferon
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
MX364957B (es) 2012-08-14 2019-05-15 10X Genomics Inc Composiciones y metodos para microcapsulas.
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
EP2971006A4 (de) * 2013-03-15 2017-02-08 Transposagen Biopharmaceuticals, Inc. Reproduzierbares verfahren für hodengesteuerte genetische modifikation und spermagesteuerte genetische modifikation
AU2014268710B2 (en) 2013-05-23 2018-10-18 The Board Of Trustees Of The Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
UY36198A (es) 2014-07-01 2016-01-29 Millennium Pharm Inc Compuestos de heteroarilo útiles como inhibidores de enzima activadora de sumo
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3262188B1 (de) 2015-02-24 2021-05-05 10X Genomics, Inc. Verfahren zur sequenzierung von nukleinsäuren in gesteuertem ausmass
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
PT3334841T (pt) 2015-08-12 2020-01-30 Cemm Forschungszentrum Fuer Molekulare Medizin Gmbh Métodos para estudar ácidos nucleicos
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
ES2924185T3 (es) 2017-09-25 2022-10-05 Fred Hutchinson Cancer Center Perfiles in situ de alta eficiencia dirigidos a todo el genoma
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
KR20220080091A (ko) 2019-09-06 2022-06-14 체엠엠 - 포르슝스첸트룸 퓨어 몰레쿨라레 메디친 게엠베하 Rna 올리고뉴클레오티드를 시퀀싱하기 위한 방법
CN114317600A (zh) * 2020-10-12 2022-04-12 上海君赛生物科技有限公司 新型PiggyBac转座子系统及其用途
WO2023131534A1 (en) * 2022-01-10 2023-07-13 Certest Biotec, S.L. Method for processing nucleic-acid containing samples

Family Cites Families (126)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4870009A (en) 1982-11-22 1989-09-26 The Salk Institute For Biological Studies Method of obtaining gene product through the generation of transgenic animals
US4670388A (en) 1982-12-30 1987-06-02 Carnegie Institution Of Washington Method of incorporating DNA into genome of drosophila
US4914025A (en) 1985-12-05 1990-04-03 Colin Manoil Export of intra-cellular substances
EP0279582A3 (de) 1987-02-17 1989-10-18 Pharming B.V. DNA-Sequenzen die Proteine zwecks effizienter Abscheidung zur Milchdrüse leiten
US5861478A (en) 1987-07-06 1999-01-19 Helix Biomedix, Inc. Lytic peptides
AU2132088A (en) 1987-07-06 1989-01-30 Louisiana State University Agricultural And Mechanical College Inhibition of eucaryotic pathogens and neoplasms and stimulation of fibroblasts and lymphocytes with lytic peptides
CA1327311C (en) 1987-07-06 1994-03-01 Jesse M. Jaynes Therapeutic antimicrobial polypeptides, their use and methods for preparation
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5212080A (en) 1987-10-05 1993-05-18 Washington University Method of DNA sequencing using DNA transposon Tn5seql
US5162215A (en) 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
AU3597093A (en) 1992-01-27 1993-09-01 Embrex Inc. Gene transfer in poultry by introduction of embryo cells (in ovo)
US5753502A (en) 1993-08-05 1998-05-19 Icos Corporation Neuron-specific ICAM-4 promoter
EP0669986B1 (de) 1992-11-13 2003-04-09 Idec Pharmaceuticals Corporation Konsensus-kozak-sequenzen zur säugetier-exprimierung
US6607884B1 (en) 1993-03-19 2003-08-19 The Johns Hopkins University School Of Medicine Methods of detecting growth differentiation factor-8
US20030074680A1 (en) * 1993-03-19 2003-04-17 Johns Hopkins University School Of Medicine Growth differentiation factor-8
US5645991A (en) 1993-05-04 1997-07-08 Univ. Of Connecticut Transposon-containing DNA cloning vector and uses thereof
US5512483A (en) 1993-05-21 1996-04-30 Mcgill University Expression vectors responsive to steroid hormones
AU7321294A (en) * 1993-06-30 1995-01-24 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College, The Transformed eukaryotic cells, and transposon-based transformation vectors
US6156568A (en) 1993-06-30 2000-12-05 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transformed eukaryotic cells
WO1995001424A1 (en) 1993-06-30 1995-01-12 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transformed mammalian cells capable of expressing cecropin b
US5648244A (en) * 1993-09-27 1997-07-15 President And Fellows Of Harvard College Production, purification, cleavage and use of fusion peptides
US5827690A (en) * 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
EP1574577A3 (de) 1994-07-08 2006-06-14 The Johns Hopkins University School Of Medicine Wachstums-Differenzierungsfaktor-11
US5843705A (en) 1995-02-21 1998-12-01 Genzyme Transgenic Corporation Transgenically produced antithrombin III
WO1996040724A1 (en) 1995-06-07 1996-12-19 Life Technologies, Inc. Recombinational cloning using engineered recombination sites
US5998698A (en) 1995-06-07 1999-12-07 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transgenic fish capable of expressing exogenous lytic peptides
CA2224907A1 (en) 1995-07-25 1997-02-13 Introgene B.V. Methods and means for targeted gene delivery
GB9518323D0 (en) * 1995-09-07 1995-11-08 Steidler Lothar Materials and methods relating to the attachment and display of substances on cell surfaces
US6218185B1 (en) 1996-04-19 2001-04-17 The United States Of America As Represented By The Secretary Of Agriculture Piggybac transposon-based genetic transformation system for insects
CA2257357C (en) 1996-06-07 2010-04-13 Neorx Corporation Humanized antibodies with modified glycosylation
US6825396B2 (en) 1996-06-12 2004-11-30 Board Of Trustees Operating Michigan State University Methods for tissue specific synthesis of protein in eggs of transgenic hens
US6563017B2 (en) 1996-07-08 2003-05-13 Dnavec Research Inc. In vivo electroporation method for early stage embryo of chickens
US6503729B1 (en) 1996-08-22 2003-01-07 The Board Of Trustees Of The University Of Illinois Selected polynucleotide and polypeptide sequences of the methanogenic archaeon, methanococcus jannashii
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US6107477A (en) 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
DE69838510T2 (de) 1997-02-20 2008-07-03 Johns Hopkins University School Of Medicine Mutationen in atp-abhängigen transpositionsproteinen die die zielortspezifität reduzieren
JP2001512976A (ja) 1997-02-25 2001-08-28 ジェンザイム・トランスジェニックス・コーポレーション トランスジェニックにより産生された非分泌性タンパク質
CA2280997C (en) 1997-03-11 2013-05-28 Perry B. Hackett Dna-based transposon system for the introduction of nucleic acid into dna of a cell
US6080912A (en) 1997-03-20 2000-06-27 Wisconsin Alumni Research Foundation Methods for creating transgenic animals
AU8589998A (en) 1997-07-24 1999-02-16 Valentis, Inc. Ghrh expression system and methods of use
WO1999005295A1 (en) 1997-07-25 1999-02-04 Thomas Jefferson University Composition and method for targeted integration into cells
US20030115622A1 (en) * 1997-08-04 2003-06-19 Ponce De Leon F. Abel Production of avian embryonic germ (eg) cell lines by prolonged culturing of pgc's, use thereof for cloning and chimerization
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
US6140129A (en) 1997-09-17 2000-10-31 Wisconsin Alumni Research Foundation Chromosomal targeting in bacteria using FLP recombinase
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7129390B2 (en) * 1997-10-16 2006-10-31 Avigenics, Inc Poultry Derived Glycosylated Interferon Alpha 2b
JP2001520009A (ja) * 1997-10-16 2001-10-30 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド 鳥の導入遺伝子についてのマグナム特異的プロモーターを含むベクター
ATE426036T1 (de) 1997-11-14 2009-04-15 Cedars Sinai Medical Center Transfektion und transfer nicht humaner mannlicher keimzellen zur generierung transgener nicht humaner saugetiere
US20020053092A1 (en) 1997-11-14 2002-05-02 Readhead Carol W. Nucleic acid constructs containing a cyclin A1 promoter, and kit
EP0935385A3 (de) * 1998-02-04 2002-06-19 Hitachi, Ltd. Dekodierungseinrichtung und Empfänger damit
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
US6291214B1 (en) 1998-05-11 2001-09-18 Glaxo Wellcome Inc. System for generating recombinant viruses
US20020013955A1 (en) 1998-06-10 2002-01-31 Sharon Ogden Production of recombinant protein in transgenic fish
ATE483017T1 (de) 1998-08-11 2010-10-15 Univ Hawaii Transgenese in säugetieren durch intracytoplasmatische injektion von spermien
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US20010036647A1 (en) * 1998-10-22 2001-11-01 Prabhakara V. Choudary Functionally assembled antigen-specific intact recombinant antibody and a method for production thereof
EP1013667B1 (de) 1998-11-09 2006-10-11 Nippon Biologicals, Inc. Verfahren zur Cytokinproduktionn unter Verwendung eines Sendai Virus Expressionssystems
US7160682B2 (en) 1998-11-13 2007-01-09 Regents Of The University Of Minnesota Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell
EP1170992A4 (de) 1998-12-04 2003-03-26 Univ Duke Gereinigte und isolierte gene der -i(piwi)-familie, ihre genprodukte; und verfahren zu ihrer verwendung
US20020148000A1 (en) 1998-12-04 2002-10-10 Shen Che-Kun James HS-40 enhancer-containing vector in transgenic animals
AU4806100A (en) 1999-04-28 2000-11-10 Board Of Trustees Of The Leland Stanford Junior University P element derived vector and methods for its use
US6518481B1 (en) * 1999-08-12 2003-02-11 Exelixis, Inc. Universal markers of transgenesis
US6773914B1 (en) 1999-08-19 2004-08-10 The United States Of America As Represented By The Secretary Of Agriculture PiggyBac transformation system
US20020055172A1 (en) 1999-10-07 2002-05-09 Harrington John J. Multiple promoter expression constructs and methods of use
JP4609869B2 (ja) 1999-12-03 2011-01-12 独立行政法人科学技術振興機構 トランスポゾン転移酵素および遺伝子改変方法
ATE391789T1 (de) 1999-12-10 2008-04-15 Invitrogen Corp Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
AU2267201A (en) 1999-12-15 2001-06-25 Paula Olhoft Method to enhance agrobacterium-mediated transformation of plants
CA2394600A1 (en) 1999-12-17 2001-06-21 Oregon Health And Science University Methods for producing transgenic animals
AU4650701A (en) * 2000-03-21 2001-10-03 Charalambos Savakis Method of generating transgenic organisms using transposons
US20030150007A1 (en) * 2000-03-21 2003-08-07 Charalambos Savakis Method of generating transgenic organisms using transposons
US6589783B2 (en) 2000-04-13 2003-07-08 Novagen, Inc. Multiple host expression vector
US7354755B2 (en) 2000-05-01 2008-04-08 Midwest Research Institute Stable zymomonas mobilis xylose and arabinose fermenting strains
EP1292285A4 (de) * 2000-06-02 2009-07-22 Eisai Corp North America Darreichungssystem für bioaktive stoffe
US20020028488A1 (en) 2000-06-19 2002-03-07 Sujay Singh Transgenic avian species for making human and chimeric antibodies
JP2002078491A (ja) 2000-06-22 2002-03-19 National Institute Of Agrobiological Sciences 青枯病菌由来の挿入配列因子
AU2001271614B2 (en) 2000-07-03 2007-05-31 Catalent Pharma Solutions, Llc Host cells containing multiple integrating vectors
WO2002004629A2 (en) 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
EP2044839A3 (de) 2000-08-03 2009-07-01 Therapeutic Human Polyclonals, Inc. Herstellung von humanisierten Antikörpern in genveränderten Tieren
GB2368064B (en) 2000-09-30 2002-11-13 Imp Cancer Res Tech DNA Element and Associated Protein
US7105343B1 (en) 2000-10-31 2006-09-12 University Of Notre Dame Du Lac Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector Piggybac
WO2002046444A2 (en) 2000-12-05 2002-06-13 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
US20020108132A1 (en) 2001-02-02 2002-08-08 Avigenics Inc. Production of a monoclonal antibody by a transgenic chicken
JP2002238559A (ja) * 2001-02-16 2002-08-27 Nagoya Industrial Science Research Inst 鳥類の卵内で外来遺伝子発現産物を生産する方法
US20020119573A1 (en) 2001-02-28 2002-08-29 Shaw Karen J. Footprinting plasmid
WO2002079447A2 (en) 2001-03-30 2002-10-10 Avigenics, Inc. Avian lysozyme promoter
US7176300B2 (en) 2001-03-30 2007-02-13 Avigenics, Inc. Avian lysozyme promoter
JP3723839B2 (ja) 2001-06-07 2005-12-07 国立大学法人広島大学 ニワトリの白血病阻止因子（ｌｉｆ）、及びそれをコードする遺伝子
WO2003022040A2 (en) * 2001-09-13 2003-03-20 California Institute Of Technology Method for producing transgenic animals
EP1438401B1 (de) * 2001-09-18 2011-06-15 Synageva BioPharma Corp. Produktion eines transgenen vogels mittels cytoplasmainjektion
US20030061629A1 (en) 2001-09-21 2003-03-27 Pramod Sutrave Production of transgenic birds using stage X primordial germ cells
US20030143740A1 (en) * 2001-10-15 2003-07-31 Christine Wooddell Processes for transposase mediated integration into mammalian cells
US7972853B2 (en) * 2001-10-22 2011-07-05 Abt Holding Company Compositions and methods for making mutations in cell lines and animals
WO2003035834A2 (en) * 2001-10-22 2003-05-01 Athersys, Inc. Compositions and methods for making mutations in cell lines and animals
US6939959B2 (en) * 2001-10-26 2005-09-06 Id Biomedical Corporation Of Washington Efficient protein expression system
US7335761B2 (en) * 2001-11-30 2008-02-26 Avigenics, Inc. Avian gene expression controlling regions
US7294507B2 (en) 2001-11-30 2007-11-13 Avigenics, Inc. Ovomucoid promoters and methods of use
US6875588B2 (en) * 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
AU2002353231B2 (en) * 2001-12-21 2008-10-16 Oxford Biomedica (Uk) Limited Method for producing a transgenic organism using a lentiviral expression vector such as EIAV
US20030121062A1 (en) * 2001-12-21 2003-06-26 Oxford Biomedica (Uk) Limited Transgenic organism
JP2006513149A (ja) * 2002-05-17 2006-04-20 ニュー ホライゾンズ ディアグノスティックス コーポレイション Ｂａｃｉｌｌｕｓａｎｔｈｒａｃｉｓを迅速かつ特異的に検出および殺滅するファージ関連溶菌酵素の同定
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
ATE494370T1 (de) * 2002-07-24 2011-01-15 Manoa Biosciences Inc Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren
GB0227645D0 (en) * 2002-11-27 2003-01-08 Viragen Inc Protein production in transgenic avians
WO2004065581A2 (en) * 2003-01-15 2004-08-05 Discovery Genomics, Inc. Transposon-insulator element delivery systems
CN1980571A (zh) * 2003-01-24 2007-06-13 阿维季尼克斯股份有限公司 禽类及禽蛋中表达的外源蛋白质
JP2006517104A (ja) * 2003-02-10 2006-07-20 マックス−デルブルック−セントラム フユール モレクラーレ メディツィン（エムディーシー） トランスポゾンに基づく標的化システム
US8420386B2 (en) * 2003-02-10 2013-04-16 Max-Delbruck-Centrum Fur Molekulare Medizin (Mdc) Transposon-based targeting system
EP1608219A4 (de) * 2003-03-07 2007-03-14 Avigenics Inc Integrase-vermittelte vogeltransgenese
US20050198700A1 (en) * 2003-03-07 2005-09-08 Avigenics, Inc. Genomic modification
US20050034186A1 (en) * 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
US20040255345A1 (en) * 2003-03-07 2004-12-16 Rapp Jeffrey C. Production of transgenic avians
US20050273873A1 (en) * 2003-03-07 2005-12-08 Avigenics, Inc. Genomic modification
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7381712B2 (en) 2003-05-09 2008-06-03 Avigenics, Inc. In vivo transfection in avians
WO2005003359A1 (ja) * 2003-07-08 2005-01-13 Japan Science And Technology Corporation トランスジェニック生物を作製する方法およびシステム
WO2005026291A2 (en) * 2003-09-12 2005-03-24 Captur Technologies Co., L.L.C. Controlling the formation of crystalline hydrates in fluid systems
US20060123504A1 (en) * 2004-12-07 2006-06-08 Avigenics, Inc. Methods of producing polyclonal antibodies
US7569223B2 (en) * 2004-03-22 2009-08-04 The Rockefeller University Phage-associated lytic enzymes for treatment of Streptococcus pneumoniae and related conditions
WO2006026238A2 (en) * 2004-08-25 2006-03-09 Avigenics, Inc. Rna interference in avians
WO2006060314A2 (en) * 2004-12-01 2006-06-08 Bayer Schering Pharma Aktiengesellschaft Generation of replication competent viruses for therapeutic use
US7657816B2 (en) * 2005-07-13 2010-02-02 Leanics Corporation Low-complexity hybrid LDPC code encoder

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