DE69026090T2 - Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration - Google Patents

Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration

Info

Publication number
DE69026090T2
DE69026090T2 DE69026090T DE69026090T DE69026090T2 DE 69026090 T2 DE69026090 T2 DE 69026090T2 DE 69026090 T DE69026090 T DE 69026090T DE 69026090 T DE69026090 T DE 69026090T DE 69026090 T2 DE69026090 T2 DE 69026090T2
Authority
DE
Germany
Prior art keywords
migration
fractionation
counter
help
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69026090T
Other languages
English (en)
Other versions
DE69026090D1 (de
Inventor
Allan Chin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of DE69026090D1 publication Critical patent/DE69026090D1/de
Application granted granted Critical
Publication of DE69026090T2 publication Critical patent/DE69026090T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44765—Apparatus specially adapted therefor of the counter-flow type
DE69026090T 1989-08-07 1990-08-06 Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration Expired - Fee Related DE69026090T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/390,631 US5096554A (en) 1989-08-07 1989-08-07 Nucleic acid fractionation by counter-migration capillary electrophoresis
PCT/US1990/004380 WO1991002244A1 (en) 1989-08-07 1990-08-06 Nucleic acid fractionation by counter-migration capillary electrophoresis

Publications (2)

Publication Number Publication Date
DE69026090D1 DE69026090D1 (de) 1996-04-25
DE69026090T2 true DE69026090T2 (de) 1996-11-14

Family

ID=23543283

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69026090T Expired - Fee Related DE69026090T2 (de) 1989-08-07 1990-08-06 Fraktionierung nukleider säuren mit hilfe von kapillarer elektrophorese in gegenmigration

Country Status (7)

Country Link
US (2) US5096554A (de)
EP (1) EP0486559B1 (de)
JP (1) JPH0797101B2 (de)
AT (1) ATE135606T1 (de)
DE (1) DE69026090T2 (de)
DK (1) DK0486559T3 (de)
WO (1) WO1991002244A1 (de)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665216A (en) * 1986-10-21 1997-09-09 Northeastern University Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5126021A (en) * 1991-07-17 1992-06-30 Applied Biosystems Inc. Low-viscosity polymer solution for capillary electrophoresis
US5213669A (en) * 1992-01-31 1993-05-25 Beckman Instruments, Inc. Capillary column containing a dynamically cross-linked composition and method of use
GB2264496B (en) * 1992-02-25 1995-10-25 Us Energy Sizing of fragments from a nucleic acid sequence
EP0582256A3 (en) * 1992-08-06 1997-09-17 Hitachi Ltd Polynucleotide detecting method and apparatus
US5409586A (en) * 1992-08-26 1995-04-25 Hitachi, Ltd. Method for analyzing nucleic acid or protein and apparatus therefor
EP0607495B1 (de) * 1992-08-26 1998-01-14 Hitachi, Ltd. Kapillar-Elektrophorese
WO1994010564A1 (de) * 1992-11-05 1994-05-11 Evotec Biosystems Gmbh Verfahren zur trennung von substanzen aus verdünnten lösungen oder suspensionen
ES2055661B1 (es) * 1993-01-20 1995-03-01 Univ Malaga Determinacion de la expresion genica por captura especifica de arn y su cuantificacion directa por electroforesis capilar en zona libre.
EP0720658A1 (de) * 1993-09-23 1996-07-10 E.I. Du Pont De Nemours And Company Elektrophoretisches verfahren zur isolierung und trennung von mikroorganismen
US5391274A (en) * 1993-10-18 1995-02-21 Beckman Instruments, Inc. Methods for controlling electroosmotic flow in coated capillary electrophoresis columns
US5891313A (en) 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US6001229A (en) * 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5573651A (en) * 1995-04-17 1996-11-12 The Dow Chemical Company Apparatus and method for flow injection analysis
US6017496A (en) 1995-06-07 2000-01-25 Irori Matrices with memories and uses thereof
US6329139B1 (en) 1995-04-25 2001-12-11 Discovery Partners International Automated sorting system for matrices with memory
US5534123A (en) * 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6110343A (en) * 1996-10-04 2000-08-29 Lockheed Martin Energy Research Corporation Material transport method and apparatus
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6537432B1 (en) 1998-02-24 2003-03-25 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO1999064836A1 (en) 1998-06-08 1999-12-16 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
JP4515640B2 (ja) 1999-02-02 2010-08-04 カリパー・ライフ・サイエンシズ・インク． タンパク質特性決定方法、装置及びシステム
US6764817B1 (en) * 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
WO2002102498A1 (en) * 2001-06-15 2002-12-27 Martin Francis J Nanopump system
JP4657524B2 (ja) * 2001-08-29 2011-03-23 嘉信 馬場 マイクロチップ電気泳動用装置及びそれを用いる電気泳動方法
JP2006505832A (ja) * 2002-02-26 2006-02-16 フアルマシア・コーポレーシヨン 配列決定システム計算機
US20030196896A1 (en) * 2002-04-17 2003-10-23 Mcwaid Thomas Harding Method and apparatus for screening flowable separation media for electrophoresis and related applications
US6887668B2 (en) * 2002-04-19 2005-05-03 Beckman Coulter, Inc. Nucleic acid separation and detection by electrophoresis with a counter-migrating high-affinity intercalating dye
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US20050034990A1 (en) * 2003-08-12 2005-02-17 Crooks Richard M. System and method for electrokinetic trapping and concentration enrichment of analytes in a microfluidic channel
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
DE602005027700D1 (de) * 2004-05-25 2011-06-09 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
DE102009009650B4 (de) 2009-02-19 2013-10-10 Atotech Deutschland Gmbh Verfahren und Vorrichtung zum Herstellen einer Kunststoffschicht sowie deren Verwendung
AU2010257118B2 (en) 2009-06-04 2014-08-28 Lockheed Martin Corporation Multiple-sample microfluidic chip for DNA analysis
CA2814720C (en) 2010-10-15 2016-12-13 Lockheed Martin Corporation Micro fluidic optic design
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
JP6102711B2 (ja) * 2013-12-10 2017-03-29 株式会社島津製作所 電気泳動分離方法
US11946900B2 (en) * 2018-04-09 2024-04-02 Shimadzu Corporation Electrophoresis flow channel cleaning method and electrophoresis device
DE102018009281A1 (de) * 2018-11-24 2020-05-28 Selectrion GmbH Verfahren und Vorrichtung zur Separation von Ionen gleicher Ladungspolarität im elektrischen Feld
CA3120895A1 (en) * 2018-12-20 2020-06-25 Sergey N. Krylov Binder selection using capillary electrophoresis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE390766B (sv) * 1972-12-19 1977-01-17 Lkb Produkter Ab Forfarande vid motflodesisotachofores
JPS60138448A (ja) * 1983-12-27 1985-07-23 Shimadzu Corp 等速電気泳動分析方法
JPS60259945A (ja) * 1984-06-05 1985-12-23 Shimadzu Corp 細管式等速電気泳動分析用リ−デイング液
US4865707A (en) * 1986-10-21 1989-09-12 Northeastern University Capillary gel electrophoresis columns
JPS63208763A (ja) * 1987-02-25 1988-08-30 Shimadzu Corp 塩基配列決定装置

Also Published As

Publication number Publication date
DE69026090D1 (de) 1996-04-25
JPH0797101B2 (ja) 1995-10-18
WO1991002244A1 (en) 1991-02-21
ATE135606T1 (de) 1996-04-15
DK0486559T3 (da) 1996-07-22
EP0486559A1 (de) 1992-05-27
JPH04507001A (ja) 1992-12-03
US5110424A (en) 1992-05-05
EP0486559B1 (de) 1996-03-20
EP0486559A4 (en) 1993-03-17
US5096554A (en) 1992-03-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8339 Ceased/non-payment of the annual fee