DE69120980T2 - Lasererregter konfokaler Mikroskop-Fluoreszenzscanner und Verfahren - Google Patents

Lasererregter konfokaler Mikroskop-Fluoreszenzscanner und Verfahren

Info

Publication number
DE69120980T2
DE69120980T2 DE69120980T DE69120980T DE69120980T2 DE 69120980 T2 DE69120980 T2 DE 69120980T2 DE 69120980 T DE69120980 T DE 69120980T DE 69120980 T DE69120980 T DE 69120980T DE 69120980 T2 DE69120980 T2 DE 69120980T2
Authority
DE
Germany
Prior art keywords
confocal microscope
fluorescence scanner
laser excited
microscope fluorescence
excited confocal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69120980T
Other languages
English (en)
Other versions
DE69120980T3 (de
DE69120980D1 (de
Inventor
Richard A Mathies
Konan Peck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27040739&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69120980(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of California filed Critical University of California
Application granted granted Critical
Publication of DE69120980D1 publication Critical patent/DE69120980D1/de
Publication of DE69120980T2 publication Critical patent/DE69120980T2/de
Publication of DE69120980T3 publication Critical patent/DE69120980T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0028—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/004—Scanning details, e.g. scanning stages fixed arrays, e.g. switchable aperture arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6473—In-line geometry
    • G01N2021/6476—Front end, i.e. backscatter, geometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/95—Detectors specially adapted therefor; Signal analysis
DE69120980T 1990-01-12 1991-01-14 Lasererregter konfokaler Mikroskop-Fluoreszenzscanner Expired - Lifetime DE69120980T3 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US46375790A 1990-01-12 1990-01-12
US463757 1990-01-12
US531900 1990-06-01
US07/531,900 US5091652A (en) 1990-01-12 1990-06-01 Laser excited confocal microscope fluorescence scanner and method

Publications (3)

Publication Number Publication Date
DE69120980D1 DE69120980D1 (de) 1996-08-29
DE69120980T2 true DE69120980T2 (de) 1997-02-27
DE69120980T3 DE69120980T3 (de) 2004-04-15

Family

ID=27040739

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69120980T Expired - Lifetime DE69120980T3 (de) 1990-01-12 1991-01-14 Lasererregter konfokaler Mikroskop-Fluoreszenzscanner

Country Status (4)

Country Link
US (1) US5091652A (de)
EP (1) EP0440342B2 (de)
JP (1) JPH06341955A (de)
DE (1) DE69120980T3 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10236433A1 (de) * 2002-08-06 2004-02-19 Förderverein Institut für Medizintechnik Dresden e.V. Vorrichtung zur optischen Detektion diskret auf einem planaren Substrat oder in Kavitäten eines Substrates angeordneten flüssigen Proben in Tropfenform

Families Citing this family (365)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5510894A (en) * 1988-12-22 1996-04-23 Renishaw Plc Spectroscopic apparatus and methods
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5925525A (en) * 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US6955915B2 (en) * 1989-06-07 2005-10-18 Affymetrix, Inc. Apparatus comprising polymers
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US6919211B1 (en) * 1989-06-07 2005-07-19 Affymetrix, Inc. Polypeptide arrays
US6416952B1 (en) 1989-06-07 2002-07-09 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
JPH083481B2 (ja) * 1989-06-07 1996-01-17 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パターン読み取り装置
US6406844B1 (en) 1989-06-07 2002-06-18 Affymetrix, Inc. Very large scale immobilized polymer synthesis
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US6506558B1 (en) 1990-03-07 2003-01-14 Affymetrix Inc. Very large scale immobilized polymer synthesis
EP0834576B1 (de) * 1990-12-06 2002-01-16 Affymetrix, Inc. (a Delaware Corporation) Detektion von Nukleinsäuresequenzen
FR2673718B1 (fr) * 1991-03-04 1993-05-14 Dilor Appareil de spectrometrie raman.
US5260578A (en) * 1991-04-10 1993-11-09 Mayo Foundation For Medical Education And Research Confocal imaging system for visible and ultraviolet light
JP3280969B2 (ja) * 1991-04-10 2002-05-13 メイヨ ファンデーション フォー メディカル エデュケーション アンド リサーチ 可視光および紫外光の共焦点結像装置
CA2042075C (en) * 1991-05-08 2001-01-23 Branko Palcic Endoscopic imaging system
WO1992019960A1 (en) 1991-05-09 1992-11-12 Nanophore, Inc. Methods for the electrophoretic separation of nucleic acids and other linear macromolecules in gel media with restrictive pore diameters
US5769792A (en) * 1991-07-03 1998-06-23 Xillix Technologies Corp. Endoscopic imaging system for diseased tissue
US6468740B1 (en) 1992-11-05 2002-10-22 Affymetrix, Inc. Cyclic and substituted immobilized molecular synthesis
US5822061A (en) * 1992-01-20 1998-10-13 Dilor Spectrometry apparatus
US5355215A (en) * 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
US5283433A (en) * 1992-10-05 1994-02-01 The Regents Of The University Of California Scanning confocal microscope providing a continuous display
JP2575270B2 (ja) * 1992-11-10 1997-01-22 浜松ホトニクス株式会社 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法
US5547849A (en) * 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
EP0622762B1 (de) * 1993-04-27 1998-07-01 The Furukawa Electric Co., Ltd. Vorrichtung zum Nachweis von Fluoreszenz
US5439578A (en) * 1993-06-03 1995-08-08 The Governors Of The University Of Alberta Multiple capillary biochemical analyzer
USH1530H (en) * 1993-06-17 1996-05-07 Ultrapointe Corporation Surface extraction from a three-dimensional data set
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
US5923430A (en) 1993-06-17 1999-07-13 Ultrapointe Corporation Method for characterizing defects on semiconductor wafers
DE4326181A1 (de) * 1993-08-04 1995-02-09 Europ Lab Molekularbiolog Verfahren und Vorrichtung zur Lumineszenzspektroskopie und Materialmikrobearbeitung von fixierten und bewegten Molekülen, Partikeln und Objekten
US5381224A (en) * 1993-08-30 1995-01-10 A. E. Dixon Scanning laser imaging system
US5538613A (en) * 1993-10-26 1996-07-23 Genesys Technologies, Inc. Electrophoresis analyzer
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6090555A (en) * 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US6741344B1 (en) * 1994-02-10 2004-05-25 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US20030017081A1 (en) * 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5631734A (en) * 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5578832A (en) * 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5537247A (en) * 1994-03-15 1996-07-16 Technical Instrument Company Single aperture confocal imaging system
US5590660A (en) * 1994-03-28 1997-01-07 Xillix Technologies Corp. Apparatus and method for imaging diseased tissue using integrated autofluorescence
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
EP0695941B1 (de) * 1994-06-08 2002-07-31 Affymetrix, Inc. Verfahren und Vorrichtung zum Verpacken von Chips
US7378236B1 (en) 1994-06-17 2008-05-27 The Board Of Trustees Of The Leland Stanford Junior University Method for analyzing gene expression patterns
US7323298B1 (en) 1994-06-17 2008-01-29 The Board Of Trustees Of The Leland Stanford Junior University Microarray for determining the relative abundances of polynuceotide sequences
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5545527A (en) * 1994-07-08 1996-08-13 Visible Genetics Inc. Method for testing for mutations in DNA from a patient sample
US5459325A (en) * 1994-07-19 1995-10-17 Molecular Dynamics, Inc. High-speed fluorescence scanner
US6800452B1 (en) 1994-08-08 2004-10-05 Science Applications International Corporation Automated methods for simultaneously performing a plurality of signal-based assays
WO1996007888A1 (en) * 1994-09-02 1996-03-14 Biometric Imaging, Inc. Calibration method and apparatus for optical scanner
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US5627022A (en) * 1994-11-01 1997-05-06 Visible Genetics Inc. Microgels for use in medical diagnosis and holders useful in fabricating same
US5483075A (en) * 1994-11-01 1996-01-09 Perkin-Elmer Corporation Rotary scanning apparatus
US5507934A (en) * 1994-11-01 1996-04-16 Visible Genetics Inc. Apparatus for preparing gels for use in electrophoretic separations and similar applications
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
US6014213A (en) * 1994-12-12 2000-01-11 Visible Genetics Inc. High dynamic range apparatus for separation and detection of polynucleotide fragments
US5550373A (en) * 1994-12-30 1996-08-27 Honeywell Inc. Fabry-Perot micro filter-detector
US5543018A (en) * 1995-02-13 1996-08-06 Visible Genetics Inc. Method and apparatus for automated electrophoresis using light polarization detector
US5784152A (en) * 1995-03-16 1998-07-21 Bio-Rad Laboratories Tunable excitation and/or tunable detection microplate reader
US5735276A (en) * 1995-03-21 1998-04-07 Lemelson; Jerome Method and apparatus for scanning and evaluating matter
US5675155A (en) * 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
US5578818A (en) * 1995-05-10 1996-11-26 Molecular Dynamics LED point scanning system
US6104945A (en) * 1995-08-01 2000-08-15 Medispectra, Inc. Spectral volume microprobe arrays
US5813987A (en) * 1995-08-01 1998-09-29 Medispectra, Inc. Spectral volume microprobe for analysis of materials
US5713364A (en) * 1995-08-01 1998-02-03 Medispectra, Inc. Spectral volume microprobe analysis of materials
FI954510A0 (fi) * 1995-09-22 1995-09-22 Labsystems Oy Analysator
FI954511A0 (fi) * 1995-09-22 1995-09-22 Labsystems Oy Fluorometer
US5599434A (en) * 1995-12-12 1997-02-04 Visible Genetics Inc. Electrophoresis gels and gel holders having adhesive affixed fiber spacers and method of making same
US5618398A (en) * 1995-12-12 1997-04-08 Visible Genetics Inc. Electrophoresis gels and gel holders having fiber spacers and method of making same
US5748491A (en) * 1995-12-20 1998-05-05 The Perkin-Elmer Corporation Deconvolution method for the analysis of data resulting from analytical separation processes
US5844598A (en) * 1996-01-17 1998-12-01 Pixel Vision, Inc. CCD based optical detector for a confocal microscope
EP0880598A4 (de) 1996-01-23 2005-02-23 Affymetrix Inc Verfahren zur analyse von nukleinsäure
US5646411A (en) * 1996-02-01 1997-07-08 Molecular Dynamics, Inc. Fluorescence imaging system compatible with macro and micro scanning objectives
US5847400A (en) * 1996-02-01 1998-12-08 Molecular Dynamics, Inc. Fluorescence imaging system having reduced background fluorescence
US5647368A (en) * 1996-02-28 1997-07-15 Xillix Technologies Corp. Imaging system for detecting diseased tissue using native fluorsecence in the gastrointestinal and respiratory tract
GB9606981D0 (en) * 1996-04-02 1996-06-05 Kodak Ltd Illumination for scanners
JP2000512744A (ja) * 1996-05-16 2000-09-26 アフィメトリックス，インコーポレイテッド 標識材料を検出するシステムおよび方法
WO1998001744A1 (en) * 1996-07-10 1998-01-15 Cambridge Imaging Limited Improved imaging system for fluorescence assays
EP0822261B1 (de) * 1996-07-29 2002-07-03 Academia Sinica Verfahren zur schnellen antimikrobiellen Empfindlichkeitsnachweis
US5751416A (en) * 1996-08-29 1998-05-12 Mississippi State University Analytical method using laser-induced breakdown spectroscopy
US6148114A (en) * 1996-11-27 2000-11-14 Ultrapointe Corporation Ring dilation and erosion techniques for digital image processing
US6066245A (en) * 1996-12-27 2000-05-23 Genetic Biosystems, Inc. Method and apparatus for scanning fluorescently labeled particles
US6847490B1 (en) * 1997-01-13 2005-01-25 Medispectra, Inc. Optical probe accessory device for use in vivo diagnostic procedures
US6826422B1 (en) * 1997-01-13 2004-11-30 Medispectra, Inc. Spectral volume microprobe arrays
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
DE19707226A1 (de) * 1997-02-24 1998-08-27 Bodenseewerk Perkin Elmer Co Lichtabtastvorrichtung
JPH10260190A (ja) * 1997-03-17 1998-09-29 Olympus Optical Co Ltd 走査型蛍光プローブ顕微鏡
US5887009A (en) * 1997-05-22 1999-03-23 Optical Biopsy Technologies, Inc. Confocal optical scanning system employing a fiber laser
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay
US6469311B1 (en) 1997-07-16 2002-10-22 Molecular Devices Corporation Detection device for light transmitted from a sensed volume
US6071748A (en) * 1997-07-16 2000-06-06 Ljl Biosystems, Inc. Light detection device
US6097025A (en) * 1997-10-31 2000-08-01 Ljl Biosystems, Inc. Light detection device having an optical-path switching mechanism
US5895915A (en) * 1997-07-24 1999-04-20 General Scanning, Inc. Bi-directional scanning system with a pixel clock system
WO1999054711A1 (en) 1998-04-17 1999-10-28 Ljl Biosystems, Inc. Sample-holding devices and systems
US6825921B1 (en) 1999-11-10 2004-11-30 Molecular Devices Corporation Multi-mode light detection system
US6576476B1 (en) 1998-09-02 2003-06-10 Ljl Biosystems, Inc. Chemiluminescence detection method and device
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6992761B2 (en) * 1997-09-20 2006-01-31 Molecular Devices Corporation Broad range light detection system
US6326605B1 (en) 1998-02-20 2001-12-04 Ljl Biosystems, Inc. Broad range light detection system
WO2000050877A1 (en) 1999-02-23 2000-08-31 Ljl Biosystems, Inc. Frequency-domain light detection device
US6982431B2 (en) * 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
WO2000006990A2 (en) 1998-07-27 2000-02-10 Ljl Biosystems, Inc. Apparatus and methods for time-resolved spectroscopic measurements
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6493459B2 (en) 1997-11-06 2002-12-10 Fuji Photo Film Co., Ltd. Image reading apparatus
US6583168B1 (en) 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6201639B1 (en) 1998-03-20 2001-03-13 James W. Overbeck Wide field of view and high speed scanning microscopy
US6100535A (en) * 1998-01-29 2000-08-08 The Regents Of The University Of California Rotary confocal scanner for detection of capillary arrays
JPH11281537A (ja) * 1998-01-30 1999-10-15 Aisin Seiki Co Ltd サンプルの特性に応じた作業方法及びこの方法に用いるサンプル台
US6475361B1 (en) 1998-02-20 2002-11-05 Tetragen Sa Capillary electrophoresis apparatus having filling/refilling system and methods for use thereof
US6103083A (en) * 1998-02-20 2000-08-15 Tetragen Capillary electrophoresis apparatus and method
WO1999044045A1 (en) * 1998-02-27 1999-09-02 Massachusetts Institute Of Technology Single molecule detection with surface-enhanced raman scattering and applications in dna or rna sequencing
US6388788B1 (en) 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
US6185030B1 (en) 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6472671B1 (en) 2000-02-09 2002-10-29 Jean I. Montagu Quantified fluorescence microscopy
US6818437B1 (en) * 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
JP2003524754A (ja) * 1998-05-16 2003-08-19 ピーイー コーポレイション （エヌワイ） Ｄｎａのポリメラーゼ連鎖反応をモニタする装置
US7498164B2 (en) 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US5880006A (en) 1998-05-22 1999-03-09 Vlsi Technology, Inc. Method for fabrication of a semiconductor device
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
DE19927724A1 (de) * 1998-06-19 2000-01-20 Optiscan Pty Ltd Verfahren und Vorrichtung mit Zweiphotonen-Endoskop oder Mikroskop
DE19829981C2 (de) † 1998-07-04 2002-10-17 Zeiss Carl Jena Gmbh Verfahren und Anordnung zur konfokalen Mikroskopie
AU5223899A (en) 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
AU5311699A (en) * 1998-07-28 2000-02-21 Ce Resources Pte Ltd Optical detection system
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6603537B1 (en) * 1998-08-21 2003-08-05 Surromed, Inc. Optical architectures for microvolume laser-scanning cytometers
US6271042B1 (en) * 1998-08-26 2001-08-07 Alpha Innotech Corporation Biochip detection system
US6103199A (en) * 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6545264B1 (en) 1998-10-30 2003-04-08 Affymetrix, Inc. Systems and methods for high performance scanning
CA2352156A1 (en) 1998-11-25 2000-06-02 Gary Cantu Single-head phosphor screen scanning systems
DE19858456A1 (de) 1998-12-18 2000-07-06 Leica Microsystems Verfahren zum Auffinden, zur Aufnahme und gegebenenfalls zur Auswertung von Objektstrukturen
US6411838B1 (en) 1998-12-23 2002-06-25 Medispectra, Inc. Systems and methods for optical examination of samples
CA2356195A1 (en) * 1998-12-23 2000-06-29 Medispectra, Inc. Optical methods and systems for cervical screening
US6270644B1 (en) 1999-01-27 2001-08-07 Affymetrix, Inc. Capillary array electrophoresis scanner
US6320196B1 (en) 1999-01-28 2001-11-20 Agilent Technologies, Inc. Multichannel high dynamic range scanner
JP4397993B2 (ja) * 1999-03-24 2010-01-13 オリンパス株式会社 顕微鏡写真撮影装置
US6937330B2 (en) 1999-04-23 2005-08-30 Ppd Biomarker Discovery Sciences, Llc Disposable optical cuvette cartridge with low fluorescence material
US6509575B1 (en) * 1999-05-07 2003-01-21 Impact Imaging, Inc. Method and apparatus for polarization filtered x-ray film scanning recording
US7423750B2 (en) * 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
US7387891B2 (en) * 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
US20050279949A1 (en) * 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7410793B2 (en) 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
WO2000079251A1 (en) * 1999-06-18 2000-12-28 Virtek Vision Corporation Elimination of crosstalk in confocal epifluorescent microarray imaging systems
AU5741800A (en) * 1999-06-18 2001-01-09 Bio-Rad Laboratories (Canada) Limited Bi-directional scanning method
US6548796B1 (en) 1999-06-23 2003-04-15 Regents Of The University Of Minnesota Confocal macroscope
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
KR100379411B1 (ko) 1999-06-28 2003-04-10 엘지전자 주식회사 바이오칩 및 그의 생체 물질 패터닝 및 측정 방법
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6687395B1 (en) * 1999-07-21 2004-02-03 Surromed, Inc. System for microvolume laser scanning cytometry
WO2001009592A1 (en) 1999-07-30 2001-02-08 California Institute Of Technology System and method for monitoring cellular activity
JP3729043B2 (ja) * 2000-08-09 2005-12-21 株式会社日立製作所 蛍光画像検出方法並びにｄｎａ検査方法及びその装置
KR100339379B1 (ko) 1999-10-29 2002-06-03 구자홍 바이오칩과 그의 생체 물질 측정장치 및 측정방법
US6471916B1 (en) * 1999-11-09 2002-10-29 Packard Instrument Company Apparatus and method for calibration of a microarray scanning system
US7187810B2 (en) * 1999-12-15 2007-03-06 Medispectra, Inc. Methods and systems for correcting image misalignment
US7260248B2 (en) * 1999-12-15 2007-08-21 Medispectra, Inc. Image processing using measures of similarity
US20020007122A1 (en) * 1999-12-15 2002-01-17 Howard Kaufman Methods of diagnosing disease
FR2806796B1 (fr) * 2000-03-23 2005-04-08 Fedaa Safadi Un systeme polyvalent pour les mesures photometriques et spectrophotometriques par l'analyse d'image
EP1182267B1 (de) * 2000-03-30 2012-01-18 Toyota Jidosha Kabushiki Kaisha Verfahren zur bestimmung der basensequenz eines einzelnen nukleinsäuremoleküls
US6690467B1 (en) 2000-05-05 2004-02-10 Pe Corporation Optical system and method for optically analyzing light from a sample
US20040224421A1 (en) * 2000-06-15 2004-11-11 Deweerd Herman Bi-directional scanning method
DE10112455C2 (de) * 2000-07-19 2003-10-23 Fraunhofer Ges Forschung Vorrichtung zur Durchführung von biochemischen Fluoreszenztests
US6603546B1 (en) * 2000-07-21 2003-08-05 I.S.S. (Usa) Inc. Rapid high throughput spectrometer and method
US7062092B2 (en) 2000-08-22 2006-06-13 Affymetrix, Inc. System, method, and computer software product for gain adjustment in biological microarray scanner
EP1186886A3 (de) * 2000-09-04 2004-01-07 Fuji Photo Film Co., Ltd. Verfahren zur biochemischen Analyse und dazu geeignetes Gerät
DE10043986B4 (de) * 2000-09-05 2020-01-16 Leica Microsystems Cms Gmbh Verfahren zur Untersuchung einer Probe und konfokales Scan-Mikroskop
AU2002213125B2 (en) * 2000-10-11 2007-11-22 Applied Biosystems, Llc. Fluorescent nucleobase conjugates having anionic linkers
EP1343973B2 (de) 2000-11-16 2020-09-16 California Institute Of Technology Vorrichtung und verfahren zur durchführung von assays und screening mit hohem durchsatz
US20020132360A1 (en) * 2000-11-17 2002-09-19 Flir Systems Boston, Inc. Apparatus and methods for infrared calorimetric measurements
US6821787B2 (en) * 2000-11-17 2004-11-23 Thermogenic Imaging, Inc. Apparatus and methods for infrared calorimetric measurements
US20040110301A1 (en) * 2000-11-17 2004-06-10 Neilson Andy C Apparatus and methods for measuring reaction byproducts
US6787761B2 (en) * 2000-11-27 2004-09-07 Surromed, Inc. Median filter for liquid chromatography-mass spectrometry data
JP3663125B2 (ja) * 2000-11-28 2005-06-22 日立ソフトウエアエンジニアリング株式会社 蛍光読み取り方法及び蛍光読み取り装置
US20020095260A1 (en) * 2000-11-28 2002-07-18 Surromed, Inc. Methods for efficiently mining broad data sets for biological markers
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6839661B2 (en) * 2000-12-15 2005-01-04 Medispectra, Inc. System for normalizing spectra
AU2001225564A1 (en) * 2000-12-29 2002-07-16 Diachip Limited An intellectual bio-chip reader for abundant diagnosis by using an optical identification code
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
AU2002307152A1 (en) * 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
WO2002095356A2 (en) * 2001-05-21 2002-11-28 Aclara Biosciences, Inc. Methods and compositions for analyzing proteins
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7280207B2 (en) * 2001-07-25 2007-10-09 Applera Corporation Time-delay integration in a flow cytometry system
EP1412724B1 (de) 2001-07-25 2016-03-23 Life Technologies Corporation Zeitverzögerungintegration in elektrophoretischen nachweissystemen
US7265833B2 (en) * 2001-07-25 2007-09-04 Applera Corporation Electrophoretic system with multi-notch filter and laser excitation source
US6873915B2 (en) * 2001-08-24 2005-03-29 Surromed, Inc. Peak selection in multidimensional data
US6750457B2 (en) * 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
US20030078739A1 (en) * 2001-10-05 2003-04-24 Surromed, Inc. Feature list extraction from data sets such as spectra
WO2003046613A2 (en) 2001-11-28 2003-06-05 Overbeck James W Scanning microscopy, fluorescence detection, and laser beam positioning
US7635588B2 (en) * 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
EP1463796B1 (de) * 2001-11-30 2013-01-09 Fluidigm Corporation Mikrofluidische vorrichtung und verfahren zu ihrer verwendung
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
EP1499706A4 (de) 2002-04-01 2010-11-03 Fluidigm Corp Mikrofluidische partikelanalysesysteme
AU2003239409A1 (en) * 2002-05-09 2003-11-11 Surromed, Inc. Methods for time-alignment of liquid chromatography-mass spectrometry data
ATE537438T1 (de) * 2002-05-17 2011-12-15 Life Technologies Corp Vorrichtung und verfahren zur unterscheidung mehrerer fluoreszenzsignale anhand ihrer anregungswellenlänge
US20060086309A1 (en) * 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
US20040208390A1 (en) * 2003-04-18 2004-10-21 Medispectra, Inc. Methods and apparatus for processing image data for use in tissue characterization
US7309867B2 (en) 2003-04-18 2007-12-18 Medispectra, Inc. Methods and apparatus for characterization of tissue samples
US7469160B2 (en) * 2003-04-18 2008-12-23 Banks Perry S Methods and apparatus for evaluating image focus
US20040208385A1 (en) * 2003-04-18 2004-10-21 Medispectra, Inc. Methods and apparatus for visually enhancing images
US7136518B2 (en) * 2003-04-18 2006-11-14 Medispectra, Inc. Methods and apparatus for displaying diagnostic data
US7459696B2 (en) 2003-04-18 2008-12-02 Schomacker Kevin T Methods and apparatus for calibrating spectral data
US7282723B2 (en) * 2002-07-09 2007-10-16 Medispectra, Inc. Methods and apparatus for processing spectral data for use in tissue characterization
US6818903B2 (en) * 2002-07-09 2004-11-16 Medispectra, Inc. Method and apparatus for identifying spectral artifacts
US6933154B2 (en) * 2002-07-09 2005-08-23 Medispectra, Inc. Optimal windows for obtaining optical data for characterization of tissue samples
US20040209237A1 (en) * 2003-04-18 2004-10-21 Medispectra, Inc. Methods and apparatus for characterization of tissue samples
US6768918B2 (en) 2002-07-10 2004-07-27 Medispectra, Inc. Fluorescent fiberoptic probe for tissue health discrimination and method of use thereof
US7103401B2 (en) 2002-07-10 2006-09-05 Medispectra, Inc. Colonic polyp discrimination by tissue fluorescence and fiberoptic probe
US20040021055A1 (en) * 2002-07-31 2004-02-05 Corson John F. Extended life biopolymer array scanner system
US20040021911A1 (en) * 2002-07-31 2004-02-05 Corson John F. Array scanner noise reduction system
US6835938B2 (en) * 2002-07-31 2004-12-28 Agilent Technologies, Inc. Biopolymer array substrate thickness dependent automated focus-distance determination method for biopolymer array scanners
EP1551753A2 (de) 2002-09-25 2005-07-13 California Institute Of Technology Hochintegriertes mikrofluidisches netzwerk
US7504072B2 (en) * 2002-09-30 2009-03-17 Agilent Technologies, Inc. Biopolymeric array scanning devices that focus on the far side of an array and methods for using the same
US7089123B2 (en) * 2002-09-30 2006-08-08 Agilent Technologies, Inc Array scanner control system
US7013220B2 (en) * 2002-09-30 2006-03-14 Agilent Technologies, Inc. Biopolymer array scanner with real-time saturation detection
US7067783B2 (en) * 2002-09-30 2006-06-27 Agilent Technologies, Inc. Method for improved focus control in molecular array scanning by using a symmetrical filter to determine in-focus-distance
JP5695287B2 (ja) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー 微小流体の核酸解析
US20040091850A1 (en) * 2002-11-08 2004-05-13 Travis Boone Single cell analysis of membrane molecules
AU2003294622A1 (en) 2002-11-18 2004-06-15 Nanofocus Ag Method for improving the signal-to-noise ratio in confocal microscopes with multi-pinhole filtering
US6913200B2 (en) * 2002-11-20 2005-07-05 Agilent Technologies, Inc. Scanning parameterization for biopolymeric array scanner
US6927389B2 (en) * 2002-12-03 2005-08-09 Agilent Technologies, Inc. Bi-directional scanner control system
US7331511B2 (en) * 2002-12-23 2008-02-19 Agilent Technologies, Inc. Biopolymeric array scanners capable of automatic scale factor selection for a plurality of different dyes, and methods for making and using the same
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US7534561B2 (en) 2003-04-02 2009-05-19 Agilent Technologies, Inc. Nucleic acid array in situ fabrication methods and arrays produced using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
WO2004102627A2 (en) * 2003-05-08 2004-11-25 Alara, Inc. Method and apparatus for radiation image erasure
US20040241667A1 (en) * 2003-05-30 2004-12-02 Chesk William G. Pulse-jet ejection head diagnostic system
TWI329208B (en) * 2003-06-03 2010-08-21 Oerlikon Trading Ag Optical substrate for enhanced detectability of fluorescence
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
WO2004109266A1 (en) * 2003-06-10 2004-12-16 Digital Bio Technology Dna detector
US7402398B2 (en) * 2003-07-17 2008-07-22 Monogram Biosciences, Inc. Measuring receptor homodimerization
JP2007502417A (ja) * 2003-08-11 2007-02-08 モノグラム バイオサイエンシズ，インコーポレーテッド 分子複合体の検出および特徴づけ
EP2259050A3 (de) 2003-08-26 2010-12-22 Blueshift Biotechnologies, Inc. Zeitabhängige Fluoreszenzmessungen
US7075100B2 (en) * 2003-09-22 2006-07-11 Honeywell International Inc. Confocal scanner system and method
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005054441A2 (en) * 2003-12-01 2005-06-16 California Institute Of Technology Device for immobilizing chemical and biomedical species and methods of using same
US6853454B1 (en) 2004-01-15 2005-02-08 Alpha Innotech Corporation Optical analysis systems
US6995901B2 (en) * 2004-01-15 2006-02-07 Alpha Innotech Corporation Optical analysis systems
US20050157299A1 (en) * 2004-01-15 2005-07-21 Heffelfinger David M. Optical analysis systems
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US7875463B2 (en) * 2004-03-26 2011-01-25 Agilent Technologies, Inc. Generalized pulse jet ejection head control model
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7248360B2 (en) * 2004-04-02 2007-07-24 Ppd Biomarker Discovery Sciences, Llc Polychronic laser scanning system and method of use
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
WO2006007207A2 (en) * 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7867707B2 (en) * 2004-06-11 2011-01-11 Evotec Ag Method for detecting analytes in a sample
US7141378B2 (en) 2004-07-02 2006-11-28 Blueshift Biotechnologies, Inc. Exploring fluorophore microenvironments
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
WO2006017642A2 (en) * 2004-08-04 2006-02-16 Agilent Technologies, Inc. Filtering of signals during array scanning
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
DE602005018840D1 (de) 2005-04-14 2010-02-25 Applied Biosystems Llc 3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sonden
US8351026B2 (en) 2005-04-22 2013-01-08 Affymetrix, Inc. Methods and devices for reading microarrays
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US20080219890A1 (en) * 2005-08-04 2008-09-11 Helicos Biosciences Corporation Sample loading and recovery
CA2617606A1 (en) * 2005-08-04 2007-02-15 Helicos Biosciences Corporation Multi-channel flow cells
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070122344A1 (en) 2005-09-02 2007-05-31 University Of Rochester Medical Center Office Of Technology Transfer Intraoperative determination of nerve location
US7530265B2 (en) * 2005-09-26 2009-05-12 Baker Hughes Incorporated Method and apparatus for elemental analysis of a fluid downhole
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
EP2363205A3 (de) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen Und Verfahren Zur Verwendung Bei Der Bildung Und Kontrolle Von Nanoreaktoren
US8055098B2 (en) * 2006-01-27 2011-11-08 Affymetrix, Inc. System, method, and product for imaging probe arrays with small feature sizes
US9445025B2 (en) 2006-01-27 2016-09-13 Affymetrix, Inc. System, method, and product for imaging probe arrays with small feature sizes
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (de) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
CA2657576C (en) 2006-07-14 2023-10-31 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
US20080161744A1 (en) 2006-09-07 2008-07-03 University Of Rochester Medical Center Pre-And Intra-Operative Localization of Penile Sentinel Nodes
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US7518727B2 (en) * 2007-02-28 2009-04-14 Beckman Coulter, Inc. Multicapillary multilaser detection system
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2009002225A2 (ru) * 2007-06-25 2008-12-31 Closed Company 'molecular-Medicine Technologies' Многофункциональное устройство для диагностики и способ тестирования биологических объектов
US9382585B2 (en) 2007-10-30 2016-07-05 Complete Genomics, Inc. Apparatus for high throughput sequencing of nucleic acids
US7988918B2 (en) 2007-11-01 2011-08-02 Complete Genomics, Inc. Structures for enhanced detection of fluorescence
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
CN101903761B (zh) * 2007-12-19 2015-04-22 皇家飞利浦电子股份有限公司 检测系统和方法
US8406860B2 (en) 2008-01-25 2013-03-26 Novadaq Technologies Inc. Method for evaluating blush in myocardial tissue
WO2009103003A2 (en) * 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Scanning fluorescent reader with diffuser system
US20090229651A1 (en) * 2008-03-14 2009-09-17 Fay Jr Theodore Denis Solar energy production system
US10219742B2 (en) 2008-04-14 2019-03-05 Novadaq Technologies ULC Locating and analyzing perforator flaps for plastic and reconstructive surgery
EP3372250B1 (de) 2008-05-02 2019-12-25 Novadaq Technologies ULC Verfahren zur herstellung und verwendung substanzbeladener erythrozyten zur beobachtung und behandlung von mikrovaskulärer hämodynamik
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
CN102224407B (zh) * 2008-11-24 2014-06-25 皇家飞利浦电子股份有限公司 用于对流体样本快速过滤分析的方法和仪器
US8374818B2 (en) * 2008-12-19 2013-02-12 Affymetrix, Inc. System, method and apparatus for calibrating inspection tools
EP3415235A1 (de) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation von mikrofluidiktröpfchen
US10492671B2 (en) 2009-05-08 2019-12-03 Novadaq Technologies ULC Near infra red fluorescence imaging for visualization of blood vessels during endoscopic harvest
US20110068279A1 (en) * 2009-08-11 2011-03-24 Fay Jr Theodore Denis Ultra dark field microscope
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (de) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digitale analyse von analyten
EP3508854A1 (de) 2010-04-27 2019-07-10 The Regents of The University of California Krebsbiomarker und verfahren zur verwendung davon
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP3709018A1 (de) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion
US8478545B2 (en) 2011-06-03 2013-07-02 Agilent Technologies, Inc. Identification of aberrant microarray features
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2850785C (en) 2011-10-06 2022-12-13 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
WO2013109981A1 (en) 2012-01-20 2013-07-25 Sequenom, Inc. Diagnostic processes that factor experimental conditions
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
CA2914778A1 (en) 2012-06-21 2013-12-27 Novadaq Technologies Inc. Quantification and analysis of angiography and perfusion
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014124006A1 (en) 2013-02-05 2014-08-14 The Johns Hopkins University Nanoparticles for magnetic resonance imaging tracking and methods of making and using thereof
EP2981921B1 (de) 2013-04-03 2023-01-18 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
IL309903A (en) 2013-05-24 2024-03-01 Sequenom Inc Methods and processes for non-invasive evaluation of genetic variations
EP3540076A1 (de) 2013-06-21 2019-09-18 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
DE102013110011B4 (de) 2013-09-12 2018-07-12 Gert Horstmeyer Verfahren und Einrichtung zur Analyse von Ölen und technischen Betriebsflüssigkeiten und zur qualifizierten Bewertung der Betriebszustände von Aggregaten
IL295860B2 (en) 2013-10-04 2024-01-01 Sequenom Inc Methods and processes for the non-invasive evaluation of genetic variations
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
JP6680680B2 (ja) 2013-10-07 2020-04-15 セクエノム， インコーポレイテッド 染色体変化の非侵襲性評価のための方法およびプロセス
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
US11783911B2 (en) 2014-07-30 2023-10-10 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
EP3201607B1 (de) 2014-09-29 2020-12-30 Novadaq Technologies ULC Bildgebung eines zielfluorophors in einem biologischen material in anwesenheit von autofluoreszenz
CA2963450A1 (en) 2014-10-09 2016-04-14 Novadaq Technologies Inc. Quantification of absolute blood flow in tissue using fluorescence-mediated photoplethysmography
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
JP6846351B2 (ja) 2015-01-27 2021-03-24 ザ・ジョンズ・ホプキンス・ユニバーシティー 粘膜表面における活性薬剤の増強された輸送のための低張ヒドロゲル製剤
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2018022890A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Genetic copy number alteration classifications
EP3574424A1 (de) 2017-01-24 2019-12-04 Sequenom, Inc. Verfahren und prozesse zur beurteilung genetischer variationen
CA3049922A1 (en) 2017-02-10 2018-08-16 Novadaq Technologies ULC Open-field handheld fluorescence imaging systems and methods
EP3641929A2 (de) 2017-06-19 2020-04-29 Massachusetts Institute Of Technology Automatisierte verfahren zur skalierbaren, parallelisierten enzymatischen biopolymersynthese und modifikation unter verwendung von mikrofluidischen vorrichtungen
WO2020056338A1 (en) 2018-09-14 2020-03-19 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
GB2589327B (en) * 2019-11-26 2023-09-13 Andor Tech Limited Differential phase contrast microscope
CN115097617B (zh) * 2022-07-15 2022-12-30 中国科学技术大学 一种基于平面芯片的全内反射荧光显微镜系统

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4087685A (en) * 1977-01-11 1978-05-02 International Business Machines Corporation Fluorescent microanalytical system and method for detecting and identifying organic materials
JPS53135660A (en) * 1977-04-30 1978-11-27 Olympus Optical Co Ltd Fluorescent photometric microscope using laser light
SU938936A1 (ru) * 1978-11-01 1982-06-30 Институт биологической физики АН СССР Устройство дл поиска измененных клеток в цитологическом препарате
US4354114A (en) * 1979-10-09 1982-10-12 Karnaukhov Valery N Apparatus for investigation of fluorescence characteristics of microscopic objects
SE455646B (sv) * 1984-10-22 1988-07-25 Radians Innova Ab Fluorescensanordning
US4656358A (en) * 1985-03-12 1987-04-07 Optoscan Corporation Laser-based wafer measuring system
DE3610165A1 (de) * 1985-03-27 1986-10-02 Olympus Optical Co., Ltd., Tokio/Tokyo Optisches abtastmikroskop
US4791310A (en) * 1986-10-02 1988-12-13 Syracuse University Fluorescence microscopy
JPS63306413A (ja) * 1987-06-09 1988-12-14 Olympus Optical Co Ltd 走査型光学顕微鏡
US4833332A (en) * 1987-06-12 1989-05-23 E. I. Du Pont De Nemours And Company Scanning fluorescent detection system
DE3742806A1 (de) * 1987-12-17 1989-07-13 Zeiss Carl Fa Verfahren und vorrichtung zur erzeugung von fluoreszenzbildern
JP2749069B2 (ja) * 1988-04-26 1998-05-13 オリンパス光学工業株式会社 蛍光顕微鏡装置
US5022757A (en) * 1989-01-23 1991-06-11 Modell Mark D Heterodyne system and method for sensing a target substance
EP0405480A3 (en) * 1989-06-28 1991-05-08 Kirin Beer Kabushiki Kaisha Method of and apparatus for detecting microorganisms

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10236433A1 (de) * 2002-08-06 2004-02-19 Förderverein Institut für Medizintechnik Dresden e.V. Vorrichtung zur optischen Detektion diskret auf einem planaren Substrat oder in Kavitäten eines Substrates angeordneten flüssigen Proben in Tropfenform

Also Published As

Publication number Publication date
EP0440342A2 (de) 1991-08-07
EP0440342B1 (de) 1996-07-24
EP0440342A3 (en) 1991-11-27
US5091652A (en) 1992-02-25
EP0440342B2 (de) 2003-08-27
JPH06341955A (ja) 1994-12-13
DE69120980T3 (de) 2004-04-15
DE69120980D1 (de) 1996-08-29

Similar Documents

Publication Publication Date Title
DE69120980T2 (de) Lasererregter konfokaler Mikroskop-Fluoreszenzscanner und Verfahren
DE69328986D1 (de) Konfokales Rastermikroskop
DE69717538T2 (de) Konfokales Rastermikroskop
DE69424271T2 (de) Verfahren zur Erregung von Farbstoffen
DE69309370D1 (de) Gerät und Verfahren zur Verbindung von optischen Fasern
NO981032L (no) Multifoton-lasermikroskopi
DE69422092D1 (de) Rastersondenmikroskop
DE69118065T2 (de) Oberflächenemittierender Halbleiterlaser und Verfahren zu seiner Herstellung
DE59602559D1 (de) Laserstrahlgerät und verfahren zur bearbeitung von werkstücken
DE69302028D1 (de) Elektrostatische isolierte Spannvorrichtung und Erregunsgsverfahren
DE69224037D1 (de) Optisches Abtastgerät und Verfahren zu seiner Programmierung
DE69635628D1 (de) Konfokales Mikroskop
DE69721167D1 (de) Verfahren zur Bilderfassung bei konfokaler Mikroskopie
DE69826800D1 (de) Integriert-optischer Wellenleiter und Verfahren zu seiner Herstellung
DE69638133D1 (de) Verfahren und Anordnungen bei optischen Fasern
DE69132860D1 (de) Halbleiterlaser und Verfahren zu seiner Herstellung
DE69531665D1 (de) Dioden-gepumpter nahezu konfokaler laser
DE68924460T2 (de) Laserabtastmikroskop und Anwendungsverfahren.
DE69116783D1 (de) Laserdiodenmodul und Verfahren zu seiner Herstellung
DE69809248D1 (de) Farbstoffzwischenverbindungen und verfahren zur herstellung von farstoffen
DE59712346D1 (de) Optischer Wellenleiter und Verfahren zu seiner Herstellung
DE10083371T1 (de) Lasermikroskop und konfokales Rastermikroskop
DE69801468D1 (de) Optische Abtastvorrichtung mit Halbleiterlaser und zugehöriges Verfahren
DE69420569D1 (de) Konfokales Durchlichtlasermikroskop
DE69419129T2 (de) Laserstrahl-Abtastevorrichtung

Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings