DE69301221D1 - Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung - Google Patents

Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung

Info

Publication number
DE69301221D1
DE69301221D1 DE69301221T DE69301221T DE69301221D1 DE 69301221 D1 DE69301221 D1 DE 69301221D1 DE 69301221 T DE69301221 T DE 69301221T DE 69301221 T DE69301221 T DE 69301221T DE 69301221 D1 DE69301221 D1 DE 69301221D1
Authority
DE
Germany
Prior art keywords
dna polymerase
diagnostic test
thermostable dna
composition containing
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69301221T
Other languages
English (en)
Other versions
DE69301221T2 (de
Inventor
Edward Robert Scalice
David John Sharkey
Kenneth George Christy
Theodore Walter Esders
John Linforth Daiss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Application granted granted Critical
Publication of DE69301221D1 publication Critical patent/DE69301221D1/de
Publication of DE69301221T2 publication Critical patent/DE69301221T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
DE69301221T 1992-10-07 1993-10-01 Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung Expired - Lifetime DE69301221T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/958,144 US5338671A (en) 1992-10-07 1992-10-07 DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody

Publications (2)

Publication Number Publication Date
DE69301221D1 true DE69301221D1 (de) 1996-02-15
DE69301221T2 DE69301221T2 (de) 1996-06-20

Family

ID=25500644

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69301221T Expired - Lifetime DE69301221T2 (de) 1992-10-07 1993-10-01 Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung

Country Status (6)

Country Link
US (2) US5338671A (de)
EP (1) EP0592035B1 (de)
JP (1) JP2647794B2 (de)
AT (1) ATE132530T1 (de)
DE (1) DE69301221T2 (de)
HK (1) HK148096A (de)

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DE69301221T2 (de) 1996-06-20
US5587287A (en) 1996-12-24
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EP0592035A3 (en) 1994-09-14
HK148096A (en) 1996-08-09

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