DE69301221T2 - Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung - Google Patents

Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung

Info

Publication number
DE69301221T2
DE69301221T2 DE69301221T DE69301221T DE69301221T2 DE 69301221 T2 DE69301221 T2 DE 69301221T2 DE 69301221 T DE69301221 T DE 69301221T DE 69301221 T DE69301221 T DE 69301221T DE 69301221 T2 DE69301221 T2 DE 69301221T2
Authority
DE
Germany
Prior art keywords
dna polymerase
diagnostic test
thermostable dna
composition containing
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69301221T
Other languages
English (en)
Other versions
DE69301221D1 (de
Inventor
Edward Robert Scalice
David John Sharkey
Kenneth George Christy
Theodore Walter Esders
John Linforth Daiss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Publication of DE69301221D1 publication Critical patent/DE69301221D1/de
Application granted granted Critical
Publication of DE69301221T2 publication Critical patent/DE69301221T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
DE69301221T 1992-10-07 1993-10-01 Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung Expired - Lifetime DE69301221T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/958,144 US5338671A (en) 1992-10-07 1992-10-07 DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody

Publications (2)

Publication Number Publication Date
DE69301221D1 DE69301221D1 (de) 1996-02-15
DE69301221T2 true DE69301221T2 (de) 1996-06-20

Family

ID=25500644

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69301221T Expired - Lifetime DE69301221T2 (de) 1992-10-07 1993-10-01 Thermostabile DNA-Polymerase-Zusammensetzung die ein Temparatur-Inhibitor enthält, diagnostische Testkits und die Verwendung

Country Status (6)

Country Link
US (2) US5338671A (de)
EP (1) EP0592035B1 (de)
JP (1) JP2647794B2 (de)
AT (1) ATE132530T1 (de)
DE (1) DE69301221T2 (de)
HK (1) HK148096A (de)

Families Citing this family (209)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5874557A (en) * 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5763173A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5693502A (en) * 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US6395888B1 (en) * 1996-02-01 2002-05-28 Gilead Sciences, Inc. High affinity nucleic acid ligands of complement system proteins
US5491086A (en) * 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
JPH09508521A (ja) * 1993-11-25 1997-09-02 パシフィック エンザイム（１９９３）リミテッド 改良型ポリメラーゼ
US5840573A (en) * 1994-02-01 1998-11-24 Fields; Robert E. Molecular analyzer and method of use
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
ES2314978T3 (es) 1994-11-02 2009-03-16 Nps Pharmaceuticals, Inc. Canales de sodio especificos del sistema ner5vioso periferico, dna que los codifica, exploracion de farmacos y metodos para obtenerlos y usarlos.
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
US7160991B1 (en) * 1995-03-02 2007-01-09 Ludwig Institute For Cancer Research Vascular endothelial growth factor polypeptides
CA2214439C (en) * 1995-03-02 2002-12-17 Amrad Operations Pty. Ltd. A novel growth factor and a genetic sequence encoding same
GB9509336D0 (en) * 1995-05-09 1995-06-28 Dynal As Chemical method
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
DE69637805D1 (de) * 1995-06-07 2009-02-26 Gilead Sciences Inc Nukleinsäure liganden die dna-polymerase binden und inhibieren
US6183967B1 (en) 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
WO1996040992A2 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
JP3527357B2 (ja) * 1996-03-29 2004-05-17 富士写真フイルム株式会社 粒子状の写真用ポリマー
JP3415995B2 (ja) * 1996-06-10 2003-06-09 科学技術振興事業団 高分子マイクロ遺伝子重合体の作成方法
AU4065397A (en) * 1996-08-14 1998-03-06 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
US5989873A (en) * 1996-09-24 1999-11-23 Vinayagamoorthy; Thuraiayah Method of detecting gene expression and/or of preventing such expression in cells
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
US6399304B1 (en) * 1996-12-20 2002-06-04 Roche Diagnostics Gmbh Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules
DE19653494A1 (de) * 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6605428B2 (en) * 1996-12-20 2003-08-12 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
EP0866071B1 (de) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modifizierte Primer
JPH10276776A (ja) * 1997-04-07 1998-10-20 Toyobo Co Ltd 可逆的に不活化された耐熱性ｄｎａポリメラーゼ
US6140086A (en) * 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
CA2270023C (en) 1998-04-28 2010-10-12 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
ATE330031T1 (de) * 1998-07-31 2006-07-15 Gen Probe Inc Reversibel inhibierende sonden
CA2283597C (en) 1998-10-02 2008-02-05 Ortho-Clinical Diagnostics, Inc. Reduced cortisol conjugates
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
DE60042969D1 (de) 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
JP4643023B2 (ja) * 1999-05-04 2011-03-02 オルソ−クリニカル ダイアグノスティクス，インコーポレイティド 細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法
WO2000079009A2 (en) 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
WO2001075139A1 (en) * 2000-04-03 2001-10-11 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
AU2001278517A1 (en) * 2000-08-03 2002-02-18 F. Hoffman-La Roche Ag Nucleic acid binding compounds containing pyrazolo(3,4-d)pyrimidine analogues of purin-2,6-diamine and their uses
US7238505B2 (en) 2000-10-04 2007-07-03 Ahram Biosystems Inc. Immobilized DNA polymerase
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US20070020622A1 (en) * 2001-09-14 2007-01-25 Invitrogen Corporation DNA Polymerases and mutants thereof
US20040106109A1 (en) * 2001-10-02 2004-06-03 Belly Robert T Detection of ras mutations
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
CA2409775C (en) * 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
AU2003247576A1 (en) * 2002-06-18 2003-12-31 Archemix Corp. Aptamer-toxin molecules and methods for using same
US20040249130A1 (en) * 2002-06-18 2004-12-09 Martin Stanton Aptamer-toxin molecules and methods for using same
EP2290095A1 (de) 2002-06-25 2011-03-02 InDex Diagnostics AB Verfahren und Kit zur Diagnose von Colitis ulcerosa
WO2004011680A1 (en) * 2002-07-25 2004-02-05 Archemix Corp. Regulated aptamer therapeutics
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
EP1539209A2 (de) * 2002-09-05 2005-06-15 Invitrogen Corporation Zusammensetzungen und verfahren zur synthetisierung von nukleinsäuren
US9303262B2 (en) * 2002-09-17 2016-04-05 Archemix Llc Methods for identifying aptamer regulators
US8853376B2 (en) 2002-11-21 2014-10-07 Archemix Llc Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics
AU2003294437A1 (en) * 2002-11-21 2004-06-18 Archemix Corporation Multivalent aptamer therapeutics with improved pharmacodynamic properties and methods of making and using the same
WO2004050899A2 (en) * 2002-12-03 2004-06-17 Archemix Corporation Method for in vitro selection of 2’-substituted nucleic acids
US20050037394A1 (en) * 2002-12-03 2005-02-17 Keefe Anthony D. Method for in vitro selection of 2'-substituted nucleic acids
EP2031070B1 (de) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplexverstärkung von Polynucleotiden
EP2390352A1 (de) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systeme und Methoden zur Erhöhung der Milchproduktion bei Tieren
US20050250106A1 (en) * 2003-04-24 2005-11-10 David Epstein Gene knock-down by intracellular expression of aptamers
DK1620568T3 (da) * 2003-04-24 2009-03-30 Afshin Ahmadian Analyse til allelspecifik mutationspåvisning
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
JP4996248B2 (ja) 2003-07-31 2012-08-08 ハンディーラブ インコーポレイテッド 粒子含有サンプルの処理
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
US7803931B2 (en) 2004-02-12 2010-09-28 Archemix Corp. Aptamer therapeutics useful in the treatment of complement-related disorders
ES2674129T3 (es) 2004-02-12 2018-06-27 Archemix Llc Agentes terapéuticos de base aptámeros útiles en el tratamiento de trastornos relacionados con complemento
WO2005112544A2 (en) 2004-02-19 2005-12-01 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
US20060193821A1 (en) * 2004-03-05 2006-08-31 Diener John L Aptamers to the human IL-12 cytokine family and their use as autoimmune disease therapeutics
US20070066550A1 (en) * 2004-03-05 2007-03-22 Diener John L Aptamers to the human IL-12 cytokine family and their use as autoimmune disease therapeutics
US20080214489A1 (en) * 2004-04-19 2008-09-04 Anthony Dominic Keefe Aptamer-mediated intracellular delivery of oligonucleotides
US7579450B2 (en) * 2004-04-26 2009-08-25 Archemix Corp. Nucleic acid ligands specific to immunoglobulin E and their use as atopic disease therapeutics
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US7622296B2 (en) * 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
CN101426908B (zh) * 2004-06-09 2014-06-11 纽星实验室 可逆性修饰的热稳定酶组合物及其制备方法和使用方法
US20060019288A1 (en) * 2004-06-30 2006-01-26 Applera Corporation Methods, reaction mixtures, and kits for ligating polynucleotides
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
AU2005282380A1 (en) * 2004-09-07 2006-03-16 Archemix Corp. Aptamer medicinal chemistry
WO2006033854A2 (en) * 2004-09-07 2006-03-30 Archemix Corp. Aptamers to von willebrand factor and their use as thrombotic disease therapeutics
US7566701B2 (en) * 2004-09-07 2009-07-28 Archemix Corp. Aptamers to von Willebrand Factor and their use as thrombotic disease therapeutics
EP1802774B1 (de) * 2004-10-18 2012-01-11 Brandeis University Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US9505846B2 (en) * 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
EP1918370B1 (de) * 2005-07-04 2014-04-16 Celestar Lexico-Sciences, Inc. Pcna-mutante
WO2007011903A2 (en) 2005-07-15 2007-01-25 Applera Corporation Analyzing messenger rna and micro rna in the same reaction mixture
WO2007011901A2 (en) 2005-07-15 2007-01-25 Applera Corporation Hot start reverse transcription by primer design
CA2624634A1 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
GB0600228D0 (en) 2006-01-06 2006-02-15 Fermentas Uab Inactivation method
WO2007096182A1 (en) * 2006-02-27 2007-08-30 Roche Diagnostics Gmbh Pcr hot start by magnesium sequestration
KR101513308B1 (ko) * 2006-03-08 2015-04-28 아케믹스 엘엘씨 안질환의 치료에 유용한 보체 결합 앱타머 및 항-ｃ5 제제
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
ES2692380T3 (es) 2006-03-24 2018-12-03 Handylab, Inc. Método para realizar PCR con un cartucho con varias pistas
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
DE102006015960A1 (de) * 2006-04-04 2007-10-11 Qiagen Gmbh Gemisch reversibel inhibierter Enzyme
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
BRPI0715427B1 (pt) * 2006-07-31 2016-04-12 Wanli Bi método para amplificação de sequência ou sinal de ácidos nucleicos alvo, oligonucleotideo modificado reversível, mistura e conjunto de reação de amplificação de ácido nucleico
KR100773561B1 (ko) * 2006-11-07 2007-11-05 삼성전자주식회사 다중 ｐｃｒ에서 비특이적 증폭을 감소시키는 장치 및 방법
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
EP2109627A4 (de) 2007-01-22 2014-07-23 Wafergen Inc Vorrichtung für chemische reaktionen mit hohem durchsatz
US20090203766A1 (en) * 2007-06-01 2009-08-13 Archemix Corp. vWF aptamer formulations and methods for use
AU2008276211B2 (en) 2007-07-13 2015-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
EP2913398B1 (de) 2007-08-27 2017-07-12 Life Technologies Corporation Verfahren für PCR
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
EP2113574A1 (de) 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2644707T3 (en) * 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2905344B1 (de) 2008-06-30 2016-11-09 Life Technologies Corporation Verfahren zur direkten Amplifikation aus rohen Nukleinsäureproben
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
WO2010105074A1 (en) 2009-03-12 2010-09-16 Brandeis University Reagents and methods for pcr
CA2755615A1 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
KR20170098985A (ko) 2009-11-19 2017-08-30 솔리스 바이오다인 폴리펩티드 안정성 및 활성을 증가시키는 조성물 및 관련된 방법
US9579402B2 (en) * 2009-12-04 2017-02-28 Biotium, Inc. Heterocycle-substituted xanthene dyes
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
EP2553463B1 (de) 2010-03-26 2017-05-03 Hongzhi Zou Verfahren und materialien zur erkennung von kolorektalneoplasmen
US9068017B2 (en) 2010-04-08 2015-06-30 Ibis Biosciences, Inc. Compositions and methods for inhibiting terminal transferase activity
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
WO2012045668A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis in a rt-pcr reaction buffer
CN103124796B (zh) 2010-10-04 2016-08-03 霍夫曼-拉罗奇有限公司 用于pcr反应缓冲液中的细胞裂解的方法
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
EP2630257B1 (de) 2010-10-22 2017-08-02 Oslo Universitetssykehus HF Verfahren und kits für den nachweis von 5-hydroxymethylcytosin
CN110129436A (zh) 2011-02-02 2019-08-16 精密科学公司 Dna甲基化的数字序列分析
WO2012120374A2 (en) 2011-03-10 2012-09-13 Oslo Universitetssykehus Hf Methods and biomarkers for detection of gastrointestinal cancers
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
CN103649298A (zh) 2011-05-12 2014-03-19 精密科学公司 核酸的分离
US8993341B2 (en) 2011-05-12 2015-03-31 Exact Sciences Corporation Removal of PCR inhibitors
US8808990B2 (en) 2011-05-12 2014-08-19 Exact Sciences Corporation Serial isolation of multiple DNA targets from stool
US9758837B2 (en) 2011-08-02 2017-09-12 The United States Of America, As Represented By The Secretary Of Agriculture Sensitive and rapid method for Candidatus Liberibacter species detection
WO2013043715A1 (en) 2011-09-19 2013-03-28 Genentech, Inc. Combination treatments comprising c-met antagonists and b-raf antagonists
ES2656961T3 (es) 2011-09-23 2018-03-01 F. Hoffmann-La Roche Ag Uso de G-Clamp para mejorar la PCR específica de alelo
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
EP2773772A1 (de) 2011-11-04 2014-09-10 Oslo Universitetssykehus HF Verfahren und biomarker zur analyse von kolorektalkarzinomen
EP3904533A1 (de) 2011-12-13 2021-11-03 Oslo Universitetssykehus HF Verfahren zur detektion des hydroxymethylierungsstatus
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
CA2860594A1 (en) 2012-01-09 2013-07-18 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
US9506117B2 (en) 2012-02-21 2016-11-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US9085761B1 (en) 2012-06-14 2015-07-21 Affymetrix, Inc. Methods and compositions for amplification of nucleic acids
EP3643793A1 (de) 2012-06-14 2020-04-29 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits für polymerasekettenreaktionen (pcr)
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
US9710596B2 (en) 2012-11-21 2017-07-18 Exact Sciences Corporation Methods for quantifying nucleic acid variations
GB201301457D0 (en) 2013-01-28 2013-03-13 Fluorogenics Ltd Freeze-dried composition
AU2014244608B2 (en) 2013-03-14 2017-09-14 Exact Sciences Corporation Detecting neoplasm
WO2014184684A2 (en) 2013-05-16 2014-11-20 Oslo Universitetssykehus Hf Methods and biomarkers for detection of hematological cancers
CA2924669C (en) 2013-09-20 2023-03-21 The Regents Of The University Of Michigan Compositions and methods for the analysis of radiosensitivity
EP3539944A1 (de) 2013-10-25 2019-09-18 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
US10253358B2 (en) 2013-11-04 2019-04-09 Exact Sciences Development Company, Llc Multiple-control calibrators for DNA quantitation
WO2015085230A1 (en) 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
WO2015095689A1 (en) 2013-12-19 2015-06-25 Exact Sciences Corporation Synthetic nucleic acid control molecules
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
CN106164261A (zh) 2014-01-22 2016-11-23 生命技术公司 适用于高温核酸合成的新颖逆转录酶
ES2812753T3 (es) 2014-03-31 2021-03-18 Mayo Found Medical Education & Res Detección de neoplasma colorectal
US10465249B2 (en) 2014-12-12 2019-11-05 Exact Sciences Development Company, Llc Method of characterizing ZDHHC1 DNA
US10011878B2 (en) 2014-12-12 2018-07-03 Exact Sciences Development Company Compositions and methods for performing methylation detection assays
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
EP3259602B9 (de) 2015-02-20 2021-05-19 Takara Bio USA, Inc. Verfahren zur schnellen präzisen abgabe, visualisierung und analyse von einzelzellen
CN115927612A (zh) 2015-03-27 2023-04-07 精密科学公司 检测食管疾病
CN104845967B (zh) 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
EP3286338B1 (de) 2015-04-24 2023-11-01 Atila Biosystems Incorporated Amplifikation mit primern einer begrenzten nukleotidzusammensetzung
CA3002196A1 (en) 2015-10-30 2017-05-04 Exact Sciences Development Company, Llc Multiplex amplification detection assay and isolation and detection of dna from plasma
EP3402826A1 (de) 2016-01-15 2018-11-21 Thermo Fisher Scientific Baltics UAB Antikörper zur bindung von thermophilen dna-polymerasen
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
CN116064796A (zh) 2016-05-05 2023-05-05 精密科学公司 通过分析甲基化dna来检测肺肿瘤
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
AU2017299597B2 (en) 2016-07-19 2023-08-24 Exact Sciences Corporation Methylated control DNA
EP3488003B1 (de) 2016-07-19 2023-10-25 Exact Sciences Corporation Nukleinsäurekontrollmoleküle von nichtmenschlichen organismen
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices
WO2018044831A1 (en) 2016-08-30 2018-03-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
EP4293128A3 (de) 2016-09-02 2024-03-20 Mayo Foundation for Medical Education and Research Erkennung eines leberzellkarzinoms
WO2018127786A1 (en) 2017-01-06 2018-07-12 Oslo Universitetssykehus Hf Compositions and methods for determining a treatment course of action
JP7289264B2 (ja) 2017-01-27 2023-06-09 エグザクト サイエンシーズ コーポレーション メチル化ｄｎａの分析による結腸腫瘍の検出
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
US11408030B2 (en) 2018-09-10 2022-08-09 Andy Madrid Test for detecting Alzheimer's disease
US20220017966A1 (en) 2018-11-16 2022-01-20 Oslo Universitetssykehus Hf Methods and compositions for characterizing bladder cancer
WO2021078813A1 (en) 2019-10-21 2021-04-29 Mip Diagnostics Limited Thermally responsive conjugates
EP4051811A4 (de) 2019-10-31 2023-12-06 Mayo Foundation for Medical Education and Research Nachweis von eierstockkrebs
WO2021242740A2 (en) 2020-05-26 2021-12-02 Qiagen Beverly Llc Polymerase enzyme
CA3188230A1 (en) 2020-08-19 2022-02-24 John B. Kisiel Detecting non-hodgkin lymphoma
CN114685671B (zh) * 2020-12-30 2023-09-26 深圳华大生命科学研究院 特异性结合Taq DNA聚合酶的单克隆抗体及其应用
CN113321733B (zh) * 2021-05-19 2022-03-08 厦门同仁心生物技术有限公司 一种TaqDNA聚合酶单克隆抗体及应用，包含该抗体的聚合酶反应体系及应用
WO2023021330A1 (en) 2021-08-16 2023-02-23 University Of Oslo Compositions and methods for determining a treatment course of action
WO2024062208A1 (en) 2022-09-20 2024-03-28 Cost-Bry Pty Ltd (trading as BiomeBank) Compositions and methods for reducing endogenous sulphide in inflammatory bowel diseases

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042862A (en) * 1975-11-28 1977-08-16 Franklin Robert C Power control for variable speed induction motors
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4638028A (en) * 1985-04-08 1987-01-20 Goodyear Tire & Rubber Company Rubber polymerases and methods for their production and use
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US5120639A (en) * 1989-04-03 1992-06-09 E. R. Squibb & Sons, Inc. Selective inhibition of dna polymerase
EP0415755B1 (de) * 1989-09-01 1995-12-20 Life Technologies Inc. Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen
EP0515506B1 (de) * 1990-02-16 2000-01-05 F. Hoffmann-La Roche Ag Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis

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DE69301221D1 (de) 1996-02-15
US5338671A (en) 1994-08-16
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ATE132530T1 (de) 1996-01-15
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