DE69301570T2 - Verfahren für die sequenzierung von synthetischen oligonukleotiden, welche internukleotid-bindungen enthalten, die nicht vom phosphodiester-typ sind - Google Patents

Verfahren für die sequenzierung von synthetischen oligonukleotiden, welche internukleotid-bindungen enthalten, die nicht vom phosphodiester-typ sind

Info

Publication number
DE69301570T2
DE69301570T2 DE69301570T DE69301570T DE69301570T2 DE 69301570 T2 DE69301570 T2 DE 69301570T2 DE 69301570 T DE69301570 T DE 69301570T DE 69301570 T DE69301570 T DE 69301570T DE 69301570 T2 DE69301570 T2 DE 69301570T2
Authority
DE
Germany
Prior art keywords
synthetic oligonucleotides
cleatide
bindings
oligonucleotides containing
containing internal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69301570T
Other languages
English (en)
Other versions
DE69301570D1 (de
Inventor
Sudhir Agrawal
Jin Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aceragen Inc
Original Assignee
Hybridon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybridon Inc filed Critical Hybridon Inc
Publication of DE69301570D1 publication Critical patent/DE69301570D1/de
Application granted granted Critical
Publication of DE69301570T2 publication Critical patent/DE69301570T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE69301570T 1992-10-06 1993-10-06 Verfahren für die sequenzierung von synthetischen oligonukleotiden, welche internukleotid-bindungen enthalten, die nicht vom phosphodiester-typ sind Expired - Fee Related DE69301570T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/958,133 US5403709A (en) 1992-10-06 1992-10-06 Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages
PCT/US1993/009592 WO1994008052A1 (en) 1992-10-06 1993-10-06 Methods for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages

Publications (2)

Publication Number Publication Date
DE69301570D1 DE69301570D1 (de) 1996-03-28
DE69301570T2 true DE69301570T2 (de) 1996-06-27

Family

ID=25500629

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69301570T Expired - Fee Related DE69301570T2 (de) 1992-10-06 1993-10-06 Verfahren für die sequenzierung von synthetischen oligonukleotiden, welche internukleotid-bindungen enthalten, die nicht vom phosphodiester-typ sind

Country Status (20)

Country Link
US (2) US5403709A (de)
EP (1) EP0664836B1 (de)
JP (1) JPH08504571A (de)
KR (1) KR950704508A (de)
AT (1) ATE134220T1 (de)
AU (1) AU670843B2 (de)
BR (1) BR9307192A (de)
CA (1) CA2146365A1 (de)
CZ (1) CZ85095A3 (de)
DE (1) DE69301570T2 (de)
DK (1) DK0664836T3 (de)
ES (1) ES2083299T3 (de)
FI (1) FI951612A (de)
GR (1) GR3019910T3 (de)
HU (1) HUT72988A (de)
NO (1) NO951328L (de)
NZ (1) NZ257032A (de)
PL (1) PL308294A1 (de)
RU (1) RU95110871A (de)
WO (1) WO1994008052A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5574141A (en) * 1991-08-28 1996-11-12 Boehringer Mannheim Gmbh Functionalized carrier materials for the simultaneous synthesis and direct labeling of oligonucleotides as primers for template-dependent enzymatic nucleic acid syntheses
US5525470A (en) * 1994-01-26 1996-06-11 Hybridon, Inc. Method of sequencing [short] oligonucleotides
US5589330A (en) * 1994-07-28 1996-12-31 Genzyme Corporation High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides
US6608035B1 (en) 1994-10-25 2003-08-19 Hybridon, Inc. Method of down-regulating gene expression
US6645943B1 (en) 1994-10-25 2003-11-11 Hybridon, Inc. Method of down-regulating gene expression
EP0718409A3 (de) * 1994-12-22 1999-02-03 Hitachi, Ltd. DNS vorbereitungsverfahren
WO1997027206A1 (en) * 1996-01-26 1997-07-31 Codon Pharmaceuticals, Inc. Oligonucleotide analogs
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US20040072210A1 (en) * 1997-07-07 2004-04-15 Billing-Medel Patricia A. Reagents and methods useful for detecting diseases of the breast
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6218118B1 (en) 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
JP2002535999A (ja) 1999-02-05 2002-10-29 アマシャム バイオサイエンス ユーケイ リミテッド ゲノム分析方法
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
EP1072679A3 (de) * 1999-07-20 2002-07-31 Agilent Technologies, Inc. (a Delaware corporation) Methode zur Herstelung von Nucleinsäuremolekülen mit einer reduzierten Sekundärstruktur
WO2003074738A1 (en) * 2000-01-18 2003-09-12 Quantom Dot Corporation Oligonucleotide-tagged semiconductor nanocrystals for microarray and fluorescence in situ hybridization
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20040219532A1 (en) * 2003-04-30 2004-11-04 Sampson Jeffrey R. Internal references measurements
US20050239089A1 (en) * 2003-06-06 2005-10-27 Johnson Martin D Mobility cassettes
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
WO2006007207A2 (en) * 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
US4797355A (en) * 1985-06-13 1989-01-10 Amgen Inc. Methods for attaching polynucleotides to supports
EP0224126A3 (de) * 1985-11-25 1989-02-01 The University of Calgary Kovalent gekoppelte komplementäre Oligodeoxynukleotide zur Verwendung als "Primer Linkers" bei Sequenzierung von Nukleinsäuren
PT92506A (pt) * 1988-12-07 1990-06-29 Gen Hospital Corp Metodo para o enriquecimento e clonagem de dna contendo uma insercao ou correspondendo a uma delecao

Also Published As

Publication number Publication date
AU670843B2 (en) 1996-08-01
CZ85095A3 (en) 1995-12-13
KR950704508A (ko) 1995-11-20
BR9307192A (pt) 1999-03-30
NO951328D0 (no) 1995-04-05
NZ257032A (en) 1997-06-24
US5403709A (en) 1995-04-04
AU5324194A (en) 1994-04-26
DE69301570D1 (de) 1996-03-28
CA2146365A1 (en) 1994-04-14
ES2083299T3 (es) 1996-04-01
ATE134220T1 (de) 1996-02-15
JPH08504571A (ja) 1996-05-21
HUT72988A (en) 1996-06-28
EP0664836A1 (de) 1995-08-02
FI951612A0 (fi) 1995-04-05
RU95110871A (ru) 1997-06-10
DK0664836T3 (da) 1996-03-11
HU9500993D0 (en) 1995-06-28
FI951612A (fi) 1995-05-10
PL308294A1 (en) 1995-07-24
US5652103A (en) 1997-07-29
NO951328L (no) 1995-05-22
EP0664836B1 (de) 1996-02-14
WO1994008052A1 (en) 1994-04-14
GR3019910T3 (en) 1996-08-31

Similar Documents

Publication Publication Date Title
DE69301570T2 (de) Verfahren für die sequenzierung von synthetischen oligonukleotiden, welche internukleotid-bindungen enthalten, die nicht vom phosphodiester-typ sind
DE68902375T2 (de) Verfahren zur vorsulfidierung von kohlenwasserstoffbehandlungskatalysatoren.
DE69311192T2 (de) Verfahren zur selektiven Hydrierung von Kohlenwasserstoffen
DE69006575D1 (de) Verfahren zur Umwandlung von Kohlenwasserstoffölen.
DE3680944D1 (de) Verfahren zur behandlung von schweren erdoelrueckstaenden.
DE68902239T2 (de) Verfahren zur entfernung von quecksilber aus kohlenwasserstoffoelen.
DE59404520D1 (de) Verfahren zur Hydroformylierung von Butadien-1.3
DE69615464T2 (de) Verfahren zur Hydrierung von Aromaten mit Chlor-Einspritzung auf Edelmetallkatalysatoren
DE68911856T2 (de) Verfahren zur Hydrogenierung von Schwerölen.
DE557527T1 (de) Verfahren zur herstellung von hochoktanbenzin.
DE69006261T2 (de) Verfahren zur Herstellung von Basisschmierölen.
DE3775426D1 (de) Verfahren zur behandlung von gasoelen.
DE69015098T2 (de) Verfahren zur Haloethylierung von aromatischen Kohlenwasserstoffen.
DE69022879D1 (de) Verfahren zur Isomerisierung von Kohlenwasserstoffen.
DE69009384T2 (de) Verfahren zur Haloethylierung von aromatischen Kohlenwasserstoffen.
DE59007835D1 (de) Verfahren zur Abtrennung von Aromaten.
DE68905673T2 (de) Verfahren zur Ausscheidung von 2,6-Dimethylnaphthalin.
ATE78861T1 (de) Verfahren zur entfernung von quecksilber aus kohlenwasserstoffoelen.
DE3871605D1 (de) Verfahren zur behandlung von polymerischen gegenstaenden durch coronaentladung.
DE69001433D1 (de) Verfahren zur vorsulfidierung von kohlenwasserstoffbehandlungskatalysatoren.
ATE87298T1 (de) Verfahren zur katalytischen hydrierung von aromatischen halogennitroverbindungen.
ATE34673T1 (de) Verfahren zum vorsulfurieren von katalysatoren fuer die behandlung von kohlenwasserstoffen.
ATE78860T1 (de) Methode zur entfernung von quecksilber aus kohlenwasserstoffoelen.
DE3768801D1 (de) Verfahren zur behandlung von metalloberflaechen.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee