DE69308162D1 - Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion - Google Patents

Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion

Info

Publication number
DE69308162D1
DE69308162D1 DE69308162T DE69308162T DE69308162D1 DE 69308162 D1 DE69308162 D1 DE 69308162D1 DE 69308162 T DE69308162 T DE 69308162T DE 69308162 T DE69308162 T DE 69308162T DE 69308162 D1 DE69308162 D1 DE 69308162D1
Authority
DE
Germany
Prior art keywords
polymerase chain
chain reaction
amplificating
sected
interlocked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69308162T
Other languages
English (en)
Other versions
DE69308162T2 (de
Inventor
Ron Grosz
Mark Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of DE69308162D1 publication Critical patent/DE69308162D1/de
Application granted granted Critical
Publication of DE69308162T2 publication Critical patent/DE69308162T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DE69308162T 1992-12-09 1993-12-08 Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion Expired - Fee Related DE69308162T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/990,297 US5340728A (en) 1992-12-09 1992-12-09 Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
PCT/US1993/011702 WO1994013832A1 (en) 1992-12-09 1993-12-08 Improved method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction

Publications (2)

Publication Number Publication Date
DE69308162D1 true DE69308162D1 (de) 1997-03-27
DE69308162T2 DE69308162T2 (de) 1997-06-26

Family

ID=25535998

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69308162T Expired - Fee Related DE69308162T2 (de) 1992-12-09 1993-12-08 Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion

Country Status (13)

Country Link
US (1) US5340728A (de)
EP (1) EP0675969B1 (de)
JP (1) JPH08504332A (de)
AT (1) ATE148925T1 (de)
AU (1) AU5736194A (de)
CA (1) CA2151036A1 (de)
DE (1) DE69308162T2 (de)
DK (1) DK0675969T3 (de)
ES (1) ES2097633T3 (de)
GR (1) GR3023325T3 (de)
IL (1) IL107935A0 (de)
MX (1) MX9307788A (de)
WO (1) WO1994013832A1 (de)

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US5556773A (en) * 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
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US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5660981A (en) * 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US5559013A (en) * 1994-06-22 1996-09-24 Johnson & Johnson Clinical Diagnostics, Inc. Method of amplification using intermediate renaturation step
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
MX9703187A (es) 1994-11-02 1998-02-28 Trophix Pharm Inc Canales de sodio especificos del sistema nervioso periferico, adn que codifica para mismos, cristalizacion, difraccion por rayos x, modelado molecular por computadora, diseño racional de farmacos seleccion de farmacos y metodos para la elaboracion y
US6110665A (en) * 1995-02-14 2000-08-29 University Of Kentucky Research Foundation Sarcocystis neuronadiagnostic primer and its use in methods of equine protozoal myeloencephalitis diagnosis
GB9509112D0 (en) * 1995-05-04 1995-06-28 Scottish Crop Research Inst Polynucleotide
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
CA2221454A1 (en) * 1995-05-19 1996-11-21 Abbott Laboratories Wide dynamic range nucleic acid detection using an aggregate primer series
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
EP0873419B1 (de) * 1995-06-06 2001-11-21 Beth Israel Deaconess Medical Center Verfahren und vorrichtung für diagnostischen dns-test
CA2223050A1 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
DE69632520T2 (de) * 1995-07-28 2005-06-02 Sapporo Breweries, Ltd. Methode für die identifizierung genetischer varianten des hopfens
US5747257A (en) * 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
DK0912766T4 (da) * 1996-06-04 2012-04-02 Univ Utah Res Found Overvågning af hybridisering under PCR
US5989873A (en) * 1996-09-24 1999-11-23 Vinayagamoorthy; Thuraiayah Method of detecting gene expression and/or of preventing such expression in cells
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
US6468743B1 (en) * 1998-05-18 2002-10-22 Conagra Grocery Products Company PCR techniques for detecting microbial contaminants in foodstuffs
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
ES2189631B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.
JP3979024B2 (ja) * 2001-03-29 2007-09-19 日本製紙株式会社 Ｒａｐｄ法による個体識別方法
MXPA03012075A (es) * 2001-06-22 2005-07-01 Marshfield Clinic Metodos y oligonucleotidos para la deteccion de salmonela sp., e. coli o157:h7 y listeria monocitogenes.
AU2003240795A1 (en) * 2002-05-24 2003-12-12 Invitrogen Corporation Nested pcr employing degradable primers
EP2031070B1 (de) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplexverstärkung von Polynucleotiden
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
BRPI0415435A (pt) * 2003-10-13 2006-12-05 Genaco Biomedical Products Inc método e kit para amplificação baseada em iniciador de ácidos nucleicos
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
KR100671501B1 (ko) * 2005-02-28 2007-01-19 삼성에버랜드 주식회사 식중독 검출용 프라이머 및 이를 이용한 세균성 식중독 검출방법
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
EP2759307B1 (de) 2006-03-29 2016-11-09 Merial Limited Impfstoff gegen Streptokokken
FR2926367B1 (fr) * 2008-01-10 2013-01-04 Novaleads Procede fluorimetrique pour evaluer l'influence d'une condition sur un echantillon biologique et ses applications.
CA2764315A1 (en) * 2009-06-02 2010-12-09 Monoquant Pty Ltd A method of nucleic acid amplification
AU2012335983B2 (en) * 2011-11-09 2018-05-31 Qualicon Diagnostics, Llc Sequences and their use for detection of Salmonella
US20230366038A1 (en) * 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
JPH0638759B2 (ja) * 1988-07-29 1994-05-25 株式会社島津製作所 サルモネラ菌検査に用いるｄｎａプローブ及びそれを用いたサルモネラ菌の検出法
EP0387696B1 (de) * 1989-03-17 1997-08-27 Abbott Laboratories Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter Reaktionskinetik
JPH04506599A (ja) * 1989-06-12 1992-11-19 セーイーエス　ビオ　アンテルナショナル 特定の核酸配列を検出する方法及びその応用
DE69032778T2 (de) * 1989-07-18 1999-07-29 Shimadzu Corp Verfahren zur Untersuchung von durch Mikroorganismen verursachten Lebensmittelvergiftungen und Reagens dafür
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process

Also Published As

Publication number Publication date
JPH08504332A (ja) 1996-05-14
IL107935A0 (en) 1994-04-12
MX9307788A (es) 1994-07-29
EP0675969A1 (de) 1995-10-11
ATE148925T1 (de) 1997-02-15
EP0675969B1 (de) 1997-02-12
US5340728A (en) 1994-08-23
AU5736194A (en) 1994-07-04
CA2151036A1 (en) 1994-06-23
ES2097633T3 (es) 1997-04-01
DE69308162T2 (de) 1997-06-26
WO1994013832A1 (en) 1994-06-23
DK0675969T3 (da) 1997-04-28
GR3023325T3 (en) 1997-08-29

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