DE69308162D1 - Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion - Google Patents
Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktionInfo
- Publication number
- DE69308162D1 DE69308162D1 DE69308162T DE69308162T DE69308162D1 DE 69308162 D1 DE69308162 D1 DE 69308162D1 DE 69308162 T DE69308162 T DE 69308162T DE 69308162 T DE69308162 T DE 69308162T DE 69308162 D1 DE69308162 D1 DE 69308162D1
- Authority
- DE
- Germany
- Prior art keywords
- polymerase chain
- chain reaction
- amplificating
- sected
- interlocked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 238000000137 annealing Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/990,297 US5340728A (en) | 1992-12-09 | 1992-12-09 | Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction |
PCT/US1993/011702 WO1994013832A1 (en) | 1992-12-09 | 1993-12-08 | Improved method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69308162D1 true DE69308162D1 (de) | 1997-03-27 |
DE69308162T2 DE69308162T2 (de) | 1997-06-26 |
Family
ID=25535998
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69308162T Expired - Fee Related DE69308162T2 (de) | 1992-12-09 | 1993-12-08 | Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion |
Country Status (13)
Country | Link |
---|---|
US (1) | US5340728A (de) |
EP (1) | EP0675969B1 (de) |
JP (1) | JPH08504332A (de) |
AT (1) | ATE148925T1 (de) |
AU (1) | AU5736194A (de) |
CA (1) | CA2151036A1 (de) |
DE (1) | DE69308162T2 (de) |
DK (1) | DK0675969T3 (de) |
ES (1) | ES2097633T3 (de) |
GR (1) | GR3023325T3 (de) |
IL (1) | IL107935A0 (de) |
MX (1) | MX9307788A (de) |
WO (1) | WO1994013832A1 (de) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5817797A (en) * | 1988-06-01 | 1998-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Sequencing DNA; a modification of the polymerase chain reaction |
US20020127548A1 (en) * | 1992-12-11 | 2002-09-12 | Christine Siedman | Methods for detecting mutations associated with hypertrophic cardiomyopathy |
US5912121A (en) * | 1992-12-11 | 1999-06-15 | Bringham And Women's Hospital | Methods for detecting mutations associated with hypertrophic cardiomyopathy |
US5556773A (en) * | 1993-08-06 | 1996-09-17 | Yourno; Joseph | Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes |
US5928905A (en) * | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
US5834252A (en) * | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
US5660981A (en) * | 1994-06-06 | 1997-08-26 | E. I. Du Pont De Nemours And Company | Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella |
US5559013A (en) * | 1994-06-22 | 1996-09-24 | Johnson & Johnson Clinical Diagnostics, Inc. | Method of amplification using intermediate renaturation step |
US5705366A (en) * | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
JP2000503521A (ja) | 1994-11-02 | 2000-03-28 | トロフィックス ファーマシューティカルズ,インコーポレイテッド | 末梢神経系特異的ナトリウムチャンネル、それをコードするdna、結晶化、x線回折、コンピューター分子モデリング、合理的薬物設計、薬物スクリーニング、ならびにその製造法および使用法 |
US6110665A (en) * | 1995-02-14 | 2000-08-29 | University Of Kentucky Research Foundation | Sarcocystis neuronadiagnostic primer and its use in methods of equine protozoal myeloencephalitis diagnosis |
GB9509112D0 (en) * | 1995-05-04 | 1995-06-28 | Scottish Crop Research Inst | Polynucleotide |
GB9509410D0 (en) * | 1995-05-10 | 1995-07-05 | Imperial College | Molecular imaging |
US5693467A (en) * | 1995-05-19 | 1997-12-02 | The American Type Culture Collection | Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers |
JPH11505126A (ja) * | 1995-05-19 | 1999-05-18 | アボツト・ラボラトリーズ | プライマーシリーズ集団を使用する広い動的範囲の核酸検出 |
JP4484967B2 (ja) * | 1995-06-06 | 2010-06-16 | ベス イスラエル ディーコネス メディカル センター | Dna診断試験の方法及び装置 |
US5882857A (en) * | 1995-06-07 | 1999-03-16 | Behringwerke Ag | Internal positive controls for nucleic acid amplification |
WO1996040992A2 (en) * | 1995-06-07 | 1996-12-19 | Abbott Laboratories | Probe masking method of reducing background in an amplification reaction |
AT402203B (de) | 1995-06-13 | 1997-03-25 | Himmler Gottfried Dipl Ing Dr | Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren |
EP0785281B1 (de) * | 1995-07-28 | 2004-05-19 | Sapporo Breweries Ltd. | Methode für die identifizierung genetischer varianten des hopfens |
US5747257A (en) * | 1996-02-29 | 1998-05-05 | E. I. Du Pont De Nemours And Company | Genetic markers and methods for the detection of escherichia coli serotype-0157:H7 |
US6458530B1 (en) * | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
EP1179600B1 (de) * | 1996-06-04 | 2005-05-11 | University Of Utah Research Foundation | Überwachung der Hybridisierung während PCR |
US5989873A (en) * | 1996-09-24 | 1999-11-23 | Vinayagamoorthy; Thuraiayah | Method of detecting gene expression and/or of preventing such expression in cells |
US5922538A (en) * | 1996-11-08 | 1999-07-13 | E.I. Du Pont De Nemours And Company | Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp |
IL120339A0 (en) * | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Improved DNA assembly method |
US6103468A (en) * | 1997-10-07 | 2000-08-15 | Labatt Brewing Company Limited | Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer |
US6468743B1 (en) * | 1998-05-18 | 2002-10-22 | Conagra Grocery Products Company | PCR techniques for detecting microbial contaminants in foodstuffs |
US6232104B1 (en) | 1999-08-17 | 2001-05-15 | Dade Behring Inc. | Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration |
ES2189631B1 (es) * | 2001-03-12 | 2005-01-01 | BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. | Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium. |
JP3979024B2 (ja) * | 2001-03-29 | 2007-09-19 | 日本製紙株式会社 | Rapd法による個体識別方法 |
NZ530343A (en) * | 2001-06-22 | 2007-01-26 | Marshfield Clinic | Methods and oligonucleotides for the detection of E. coli 0157:H7 |
AU2003240795A1 (en) * | 2002-05-24 | 2003-12-12 | Invitrogen Corporation | Nested pcr employing degradable primers |
AU2003298706A1 (en) | 2002-12-04 | 2004-06-23 | Applera Corporation | Multiplex amplification of polynucleotides |
WO2005029041A2 (en) * | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
US20060024690A1 (en) * | 2003-09-19 | 2006-02-02 | Kao H P | Normalization of data using controls |
AU2004282555B2 (en) * | 2003-10-13 | 2009-05-28 | Diatherix Laboratories, Inc. | Method and kit for primer based amplification of nucleic acids |
US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
US20060003337A1 (en) * | 2004-06-30 | 2006-01-05 | John Brandis | Detection of small RNAS |
KR100671501B1 (ko) * | 2005-02-28 | 2007-01-19 | 삼성에버랜드 주식회사 | 식중독 검출용 프라이머 및 이를 이용한 세균성 식중독 검출방법 |
US20060240442A1 (en) * | 2005-04-20 | 2006-10-26 | Vevea Dirk N | Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes |
AU2007233252C1 (en) | 2006-03-29 | 2013-05-23 | Boehringer Ingelheim Animal Health USA Inc. | Vaccine against Streptococci |
FR2926367B1 (fr) * | 2008-01-10 | 2013-01-04 | Novaleads | Procede fluorimetrique pour evaluer l'influence d'une condition sur un echantillon biologique et ses applications. |
WO2010139010A1 (en) * | 2009-06-02 | 2010-12-09 | Monoquant Pty Ltd | A method of nucleic acid amplification |
AU2012335983B2 (en) * | 2011-11-09 | 2018-05-31 | Qualicon Diagnostics, Llc | Sequences and their use for detection of Salmonella |
US20230366038A1 (en) * | 2022-05-16 | 2023-11-16 | Bioo Scientific Corporation | Compositions and methods relating to loop mediated isothermal amplification (lamp) |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
JPH0638759B2 (ja) * | 1988-07-29 | 1994-05-25 | 株式会社島津製作所 | サルモネラ菌検査に用いるdnaプローブ及びそれを用いたサルモネラ菌の検出法 |
AU636875B2 (en) * | 1989-03-17 | 1993-05-13 | Abbott Laboratories | Method and device for improved reaction kinetics in nucleic acid hybridizations |
WO1990015881A1 (fr) * | 1989-06-12 | 1990-12-27 | Cis Bio International | Procede de detection de sequences specifiques d'acides nucleiques et ses applications |
EP0409159B1 (de) * | 1989-07-18 | 1998-11-25 | Shimadzu Corporation | Verfahren zur Untersuchung von durch Mikroorganismen verursachten Lebensmittelvergiftungen und Reagens dafür |
US5126239A (en) * | 1990-03-14 | 1992-06-30 | E. I. Du Pont De Nemours And Company | Process for detecting polymorphisms on the basis of nucleotide differences |
JPH0491790A (ja) * | 1990-08-02 | 1992-03-25 | Shionogi & Co Ltd | 2段階pcr法 |
GB9107124D0 (en) * | 1991-04-05 | 1991-05-22 | Dynal As | Chemical process |
-
1992
- 1992-12-09 US US07/990,297 patent/US5340728A/en not_active Expired - Lifetime
-
1993
- 1993-12-08 IL IL10793593A patent/IL107935A0/xx unknown
- 1993-12-08 JP JP6514246A patent/JPH08504332A/ja active Pending
- 1993-12-08 DE DE69308162T patent/DE69308162T2/de not_active Expired - Fee Related
- 1993-12-08 EP EP94903404A patent/EP0675969B1/de not_active Expired - Lifetime
- 1993-12-08 WO PCT/US1993/011702 patent/WO1994013832A1/en active IP Right Grant
- 1993-12-08 AT AT94903404T patent/ATE148925T1/de not_active IP Right Cessation
- 1993-12-08 ES ES94903404T patent/ES2097633T3/es not_active Expired - Lifetime
- 1993-12-08 CA CA002151036A patent/CA2151036A1/en not_active Abandoned
- 1993-12-08 DK DK94903404.5T patent/DK0675969T3/da active
- 1993-12-08 AU AU57361/94A patent/AU5736194A/en not_active Abandoned
- 1993-12-09 MX MX9307788A patent/MX9307788A/es unknown
-
1997
- 1997-05-02 GR GR970400984T patent/GR3023325T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
WO1994013832A1 (en) | 1994-06-23 |
ES2097633T3 (es) | 1997-04-01 |
EP0675969A1 (de) | 1995-10-11 |
US5340728A (en) | 1994-08-23 |
AU5736194A (en) | 1994-07-04 |
IL107935A0 (en) | 1994-04-12 |
EP0675969B1 (de) | 1997-02-12 |
MX9307788A (es) | 1994-07-29 |
DE69308162T2 (de) | 1997-06-26 |
DK0675969T3 (da) | 1997-04-28 |
ATE148925T1 (de) | 1997-02-15 |
GR3023325T3 (en) | 1997-08-29 |
JPH08504332A (ja) | 1996-05-14 |
CA2151036A1 (en) | 1994-06-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |