DE69419248D1 - Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung - Google Patents

Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung

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Publication number
DE69419248D1
DE69419248D1 DE69419248T DE69419248T DE69419248D1 DE 69419248 D1 DE69419248 D1 DE 69419248D1 DE 69419248 T DE69419248 T DE 69419248T DE 69419248 T DE69419248 T DE 69419248T DE 69419248 D1 DE69419248 D1 DE 69419248D1
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Germany
Prior art keywords
dna polymerase
amino acid
dna
primer extension
dna polymerases
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE69419248T
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English (en)
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DE69419248T2 (de
Inventor
Wayne M Barnes
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Takara Bio Inc
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Takara Shuzo Co Ltd
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Publication of DE69419248T2 publication Critical patent/DE69419248T2/de
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69419248T 1993-02-19 1994-02-22 Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung Expired - Lifetime DE69419248T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/021,623 US5436149A (en) 1993-02-19 1993-02-19 Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
PCT/US1994/001867 WO1994026766A1 (en) 1993-02-19 1994-02-22 Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension

Publications (2)

Publication Number Publication Date
DE69419248D1 true DE69419248D1 (de) 1999-07-29
DE69419248T2 DE69419248T2 (de) 2000-01-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE69419248T Expired - Lifetime DE69419248T2 (de) 1993-02-19 1994-02-22 Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung

Country Status (10)

Country Link
US (1) US5436149A (de)
EP (1) EP0693078B1 (de)
JP (2) JP2885324B2 (de)
AT (1) ATE181573T1 (de)
AU (1) AU671204B2 (de)
DE (1) DE69419248T2 (de)
DK (1) DK0693078T3 (de)
ES (1) ES2136730T3 (de)
NZ (1) NZ262663A (de)
WO (1) WO1994026766A1 (de)

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