DE69428855D1 - Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung - Google Patents

Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung

Info

Publication number
DE69428855D1
DE69428855D1 DE69428855T DE69428855T DE69428855D1 DE 69428855 D1 DE69428855 D1 DE 69428855D1 DE 69428855 T DE69428855 T DE 69428855T DE 69428855 T DE69428855 T DE 69428855T DE 69428855 D1 DE69428855 D1 DE 69428855D1
Authority
DE
Germany
Prior art keywords
pcr
measurement
genes
determination
kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69428855T
Other languages
English (en)
Other versions
DE69428855T2 (de
Inventor
Nobuko Yamamoto
Tadashi Okamoto
Yoshinori Tomida
Tetsuya Yano
Takeshi Miyazaki
Masahiro Kawaguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Canon Inc
Original Assignee
Canon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP02289594A external-priority patent/JP3683913B2/ja
Application filed by Canon Inc filed Critical Canon Inc
Publication of DE69428855D1 publication Critical patent/DE69428855D1/de
Application granted granted Critical
Publication of DE69428855T2 publication Critical patent/DE69428855T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
DE69428855T 1993-09-13 1994-06-21 Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung Expired - Lifetime DE69428855T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP22720493 1993-09-13
JP02289594A JP3683913B2 (ja) 1993-03-10 1994-02-21 Ｐｃｒによる核酸定量方法、ｐｃｒによる微生物または細胞の数、特定遺伝子数及び特定遺伝子コピー数の計測法、ならびにこれらの方法に用いる測定用キット

Publications (2)

Publication Number Publication Date
DE69428855D1 true DE69428855D1 (de) 2001-12-06
DE69428855T2 DE69428855T2 (de) 2002-05-02

Family

ID=26360189

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69428855T Expired - Lifetime DE69428855T2 (de) 1993-09-13 1994-06-21 Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung

Country Status (5)

Country Link
US (1) US5670315A (de)
EP (1) EP0643140B1 (de)
AT (1) ATE207966T1 (de)
CA (1) CA2126391C (de)
DE (1) DE69428855T2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US7273749B1 (en) 1990-06-04 2007-09-25 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
JPH07233065A (ja) * 1993-12-27 1995-09-05 Canon Inc ピリリウム塩又はピリリウム類似塩を含む光化学治療薬
EP0684239B1 (de) * 1994-05-26 2003-12-10 Canon Kabushiki Kaisha Verfahren zum Nachweis von einer Zielsubstanz in einer Probe mit Hilfe von Pyryliumverbindung
US5955268A (en) * 1996-04-26 1999-09-21 Abbott Laboratories Method and reagent for detecting multiple nucleic acid sequences in a test sample
EP1179600B1 (de) 1996-06-04 2005-05-11 University Of Utah Research Foundation Überwachung der Hybridisierung während PCR
ATE230801T1 (de) 1996-10-03 2003-01-15 Canon Kk Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
WO1998036096A1 (en) * 1997-02-14 1998-08-20 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JPH11341989A (ja) * 1998-03-31 1999-12-14 Sanyo Electric Co Ltd Ｄｎａ断片増幅方法、ｄｎａ断片増幅装置、微生物群測定方法、微生物群分析方法および汚染物質測定方法
US6960432B2 (en) * 1999-07-26 2005-11-01 Canon Kabushiki Kaisha Detection/quantification of targeted nucleotide chains, and detection/quantification of multi-stranded nucleotide chains by fluorescence
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
JP3596868B2 (ja) * 2000-11-24 2004-12-02 キヤノン株式会社 末端標識プローブアレイのプローブの量の評価方法、及び、該標識プローブアレイを用いた標的物質量の評価方法
US20040028665A1 (en) * 2002-01-08 2004-02-12 Garner Bryan E. Compositions and methods for inhibiting pathogenic growth
JP2004069677A (ja) 2002-06-13 2004-03-04 Canon Inc 免疫学的測定方法、免疫学的測定用試薬及びその製造方法
WO2004062388A1 (en) * 2003-01-06 2004-07-29 Nutrition Physiology Corporation Compositions and methods for reducing the pathogen content of meat and meat products
JP4458327B2 (ja) * 2003-08-28 2010-04-28 キヤノン株式会社 標的物質の定量方法および該方法に用いるプローブ担体
WO2005022119A2 (en) * 2003-08-29 2005-03-10 Nutrition Physiology Corporation Methods for detecting and quantifying specific microorganisms
US20050048515A1 (en) * 2003-08-29 2005-03-03 Garner Bryan E. Methods for detecting and quantifying specific probiotic microorganisms in animal feed
US7419820B2 (en) * 2003-12-16 2008-09-02 Canon Kabushiki Kaisha Transfer sheet for transferring biologically active substance to culture plate
JP4632400B2 (ja) * 2003-12-16 2011-02-16 キヤノン株式会社 細胞培養用基板、その製造方法、それを用いた細胞スクリーニング法
JP4541731B2 (ja) * 2004-03-12 2010-09-08 キヤノン株式会社 核酸検出方法
JP4147235B2 (ja) * 2004-09-27 2008-09-10 キヤノン株式会社 吐出用液体、吐出方法、液滴化方法、液体吐出カートリッジ及び吐出装置
JP2006094760A (ja) * 2004-09-29 2006-04-13 Canon Inc 細胞のスクリーニング方法および口腔癌細胞検出方法
US8535914B2 (en) * 2005-01-21 2013-09-17 Canon Kabushiki Kaisha Probe, probe set and information acquisition method using the same
JP2006204255A (ja) * 2005-01-31 2006-08-10 Canon Inc アセチル−ＣｏＡアシルトランスフェラーゼ遺伝子破壊ポリヒドロキシアルカノエート生産菌、またこれを利用したポリヒドロキシアルカノエート生産方法
JP2006204258A (ja) * 2005-01-31 2006-08-10 Canon Inc 新規ポリヒドロキシアルカノエート合成微生物及びそれを用いたポリヒドロキシアルカノエートの製造方法
JP2006204257A (ja) 2005-01-31 2006-08-10 Canon Inc ポリヒドロキシアルカノエート合成酵素遺伝子の破壊されたポリヒドロキシアルカノエート生産菌の同質遺伝子系統、またこれを利用したポリヒドロキシアルカノエート生産方法
JP4689340B2 (ja) * 2005-05-02 2011-05-25 キヤノン株式会社 吐出用液体医薬組成物
JP2007010413A (ja) * 2005-06-29 2007-01-18 Canon Inc 核酸ハイブリッドの融点測定方法及びそのための装置
US7642086B2 (en) * 2005-08-09 2010-01-05 Canon Kabushiki Kaisha Labeled probe bound object, method for producing the same and method for using the same
JP2007163185A (ja) * 2005-12-09 2007-06-28 Canon Inc 酵素電極
JP2007163268A (ja) * 2005-12-13 2007-06-28 Canon Inc 酵素電極
US20090011413A1 (en) * 2005-12-14 2009-01-08 Canon Kabushiki Kaisha Method for screening colon cancer cells and gene set used for examination of colon cancer
US8133668B2 (en) * 2006-03-31 2012-03-13 Canon Kabushiki Kaisha Probe, probe set, probe-immobilized carrier, and genetic testing method
US8187803B2 (en) * 2006-03-31 2012-05-29 Canon Kabushiki Kaisha Probe, probe set, probe-immobilized carrier, and genetic testing method
WO2009011674A1 (en) * 2007-07-13 2009-01-22 Western Michigan University Research Foundation Trans-1,2-diphenylethylene derivatives and nanosensors made therefrom
US8093060B2 (en) * 2008-02-28 2012-01-10 Canon Kabushiki Kaisha Multisite phosphorylated peptide (protein) recognizing compound and detection method, imaging method, alzheimer's disease diagnosing method and reagent kit using the same
JP5688899B2 (ja) 2008-12-25 2015-03-25 キヤノン株式会社 生物試料用標識剤並びに該標識剤を用いた標識方法及びスクリーニング方法
JP5729904B2 (ja) 2009-06-02 2015-06-03 キヤノン株式会社 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法
EP2465934A1 (de) 2010-12-20 2012-06-20 Steffen Mergemeier Extraktion von Nukleinsäuren
CN110015967B (zh) * 2018-01-10 2020-10-23 中国科学院化学研究所 乙烯酮类化合物及其制备方法和应用
BR112021003156A2 (pt) 2018-08-23 2021-05-11 Seagen, Inc. composição, anticorpo que se liga ao tigit humano, formulação farmacêutica, polinucleotídeo isolado, vetor, célula hospedeira, métodos para a produção de um anticorpo que se liga ao tigit humano e de anticorpos afucosilados que se ligam a tigit e para o tratamento de um câncer, e, kit.
CN110499378A (zh) * 2019-09-12 2019-11-26 卓源健康科技有限公司 一种从临床血液中检测大肠埃希菌病原菌的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4555396A (en) * 1982-12-22 1985-11-26 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4840784A (en) * 1982-12-22 1989-06-20 Eastman Kodak Company Use of pyrylium and thiapyrylium compounds as biological stains
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
ATE88761T1 (de) * 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
AU622426B2 (en) * 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
CA2033692A1 (en) * 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
US5102784A (en) * 1990-05-04 1992-04-07 Oncor, Inc. Restriction amplification assay
DE69128520T2 (de) * 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
JP3247001B2 (ja) * 1992-12-21 2002-01-15 キヤノン株式会社 ピリリウム化合物を用いた２本鎖核酸の検出方法、ピリリウム化合物を含有するプローブ及びそれを用いた標的核酸の検出方法、新規ピリリウム化合物

Also Published As

Publication number Publication date
US5670315A (en) 1997-09-23
CA2126391C (en) 2002-01-08
ATE207966T1 (de) 2001-11-15
CA2126391A1 (en) 1995-03-14
EP0643140B1 (de) 2001-10-31
EP0643140A1 (de) 1995-03-15
DE69428855T2 (de) 2002-05-02

Similar Documents

Publication Publication Date Title
DE69428855T2 (de) Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung
ATE115959T1 (de) Markiertes dna.
ATE386139T1 (de) Verfahren, basierend auf einer polynukleotidmatrix, zur identifizierung von mikroorganismen
ES8605862A1 (es) Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad
DE3850093D1 (de) Verfahren zur Amplifizierung von Nukleinsäuren.
NO870613L (no) Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
DE19732086A1 (de) Verfahren zur quantitativen Bestimmung von Eubakterien
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
DE3650043T2 (de) Nachweis von Mykoplasma durch Hybridisierung von DNS.
DE60115205D1 (de) Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung
Rudney et al. Endpoint quantitative PCR assays for Bacteroides forsythus, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans
Subak-Sharpe Base doublet frequency patterns in the nucleic acid and evolution of viruses
ES8600404A1 (es) Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico.
SE8700821D0 (sv) Quantitative determination of nucleic acid molecules and the reagent kit used
ATE48140T1 (de) Modifizierte nukleotide und verfahren zu ihrer herstellung und anwendung.
ATE227345T1 (de) Verfahren zur gattungs- und speziesspezifische identifizierung von legionellen
DE3485811T2 (de) Molekulare genetische sonde und verfahren zu ihrer herstellung, testmethode und satz in denen diese molekulare genetische sonde gebraucht wird.
PT82940A (de) Mit uberzugen versehene formkorper zur bindung von bioaffinen substanzen verfahren zu ihrer herstellung sowie ihre verwendung
EP0231126A3 (de) Fluoreszierende Farbstoffe und deren biologische und analytische Verwendung
CN112074613A (zh) 用于复杂基因库的pcr的多种特异性/非特异性引物
WO2000058521A3 (en) Methods for the identification of reporter and target molecules using comprehensive gene expression profiles
Robertson et al. The comparison of differences in reiterated sequences by RNA-DNA hybridisation
ATE108834T1 (de) Spezifische nukleinsäurefragmente des menschlichen villingens, ihre anwendung für diagnostische zwecke.
Eisenstein et al. Gene probes for bacteria: Edited by AJL Macario and E. Conway de Macario. San Diego: Academic Press.(1990). 515 pp. $89.00
Saunders The molecular architecture of yeast centromeres.