DE69434358D1 - Methode zur herstellung der proteinase nla aus tabak - Google Patents

Methode zur herstellung der proteinase nla aus tabak

Info

Publication number
DE69434358D1
DE69434358D1 DE69434358T DE69434358T DE69434358D1 DE 69434358 D1 DE69434358 D1 DE 69434358D1 DE 69434358 T DE69434358 T DE 69434358T DE 69434358 T DE69434358 T DE 69434358T DE 69434358 D1 DE69434358 D1 DE 69434358D1
Authority
DE
Germany
Prior art keywords
plant virus
proteinase
proteins
nla
tobacco
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434358T
Other languages
English (en)
Inventor
Stephen A Johnston
William G Dougherty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oregon State University
University of Texas System
Original Assignee
Oregon State University
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oregon State University, University of Texas System filed Critical Oregon State University
Application granted granted Critical
Publication of DE69434358D1 publication Critical patent/DE69434358D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
DE69434358T 1993-02-12 1994-01-31 Methode zur herstellung der proteinase nla aus tabak Expired - Lifetime DE69434358D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/021,603 US5532142A (en) 1993-02-12 1993-02-12 Method of isolation and purification of fusion polypeptides
PCT/US1994/001176 WO1994018331A2 (en) 1993-02-12 1994-01-31 Fusion proteins including a cleavage site recognized by a plant virus protease

Publications (1)

Publication Number Publication Date
DE69434358D1 true DE69434358D1 (de) 2005-06-09

Family

ID=21805141

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434358T Expired - Lifetime DE69434358D1 (de) 1993-02-12 1994-01-31 Methode zur herstellung der proteinase nla aus tabak

Country Status (6)

Country Link
US (1) US5532142A (de)
EP (1) EP0682709B1 (de)
AT (1) ATE294870T1 (de)
AU (1) AU6132294A (de)
DE (1) DE69434358D1 (de)
WO (1) WO1994018331A2 (de)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
US5593877A (en) * 1993-03-11 1997-01-14 The Rockefeller University Nucleic acid and recombinant production of vespid venom hyaluronidase
US5955647A (en) 1994-02-03 1999-09-21 The Scripps Research Institute Method for using tobacco mosaic virus to overproduce peptides and proteins
JP3637087B2 (ja) * 1994-11-28 2005-04-06 和男 鈴木 新規な白血球活性化因子
US5795587A (en) * 1995-01-23 1998-08-18 University Of Pittsburgh Stable lipid-comprising drug delivery complexes and methods for their production
EP0817647A4 (de) * 1995-03-24 2002-10-30 Promega Corp Behandlung von verotoxin-produzierenden escherichia coli
JPH11505421A (ja) 1995-05-11 1999-05-21 ヒューマン ジノーム サイエンシーズ，インコーポレイテッド ヒトウリジンジホスフェートガラクトース−４−エピメラーゼ
US20030069173A1 (en) * 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
US6051429A (en) 1995-06-07 2000-04-18 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
JP2000510332A (ja) * 1996-04-19 2000-08-15 ヘンリー エム．ジャクソン ファンデーション フォー ザ アドバンスメント オブ ミリタリー メディシン インチミンを単独で、または１以上の他の抗原との融合タンパク質として発現する宿主生物の投与による、免疫応答を刺激する方法
US20030040038A1 (en) * 1997-08-22 2003-02-27 Steven F. Dowdy Inducible regulatory system and use thereof
US6960457B1 (en) 1997-09-04 2005-11-01 Stanford University Reversible immobilization of arginine-tagged moieties on a silicate surface
US6221355B1 (en) 1997-12-10 2001-04-24 Washington University Anti-pathogen system and methods of use thereof
WO1999040434A1 (en) 1998-02-04 1999-08-12 Invitrogen Corporation Microarrays and uses therefor
US6506595B2 (en) 1998-03-31 2003-01-14 Itoham Foods Inc. DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs
US6897073B2 (en) * 1998-07-14 2005-05-24 Zyomyx, Inc. Non-specific binding resistant protein arrays and methods for making the same
US20030138973A1 (en) * 1998-07-14 2003-07-24 Peter Wagner Microdevices for screening biomolecules
US6576478B1 (en) * 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US6406921B1 (en) * 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6913749B2 (en) * 1998-11-02 2005-07-05 Resistentia Pharmaceuticals Ab Immunogenic polypeptides for inducing anti-self IgE responses
DK1129064T3 (da) 1998-11-12 2008-04-28 Invitrogen Corp Transfektionsreagenser
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
AU2001259512B2 (en) * 2000-05-04 2007-03-01 Yale University High density protein arrays for screening of protein activity
WO2001096539A2 (en) 2000-06-15 2001-12-20 Yale University Mutant nia proteinase with reduced self-cleavage activity and method of purification
ATE526419T1 (de) * 2002-04-22 2011-10-15 Danisco Us Inc Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren
US7825227B2 (en) * 2002-05-09 2010-11-02 Prolexys Pharmaceuticals, Inc. Method for purification of a protein complex and identification of its components
WO2004063342A2 (en) * 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
JP4850698B2 (ja) 2003-04-25 2012-01-11 ノース・キャロライナ・ステイト・ユニヴァーシティ 細胞表面タンパク質相同体をコードするアシドフィルス菌核酸配列及びその使用
EP2322540A1 (de) 2003-06-23 2011-05-18 North Carolina State University Fructo-Oligosaccharidverbrauchsverbindungen codierende Lactobacillus-acidophilus-Nukleinsäuren und Verwendungen dafür
EP1718664A4 (de) * 2004-02-02 2008-08-13 Ambrx Inc Modifizierte menschliche vierhelix-bündel-peptide und ihre verwendungen
US7459289B2 (en) 2004-03-08 2008-12-02 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor
US7632924B2 (en) * 2004-06-18 2009-12-15 Ambrx, Inc. Antigen-binding polypeptides and their uses
EP1771573A4 (de) * 2004-07-21 2009-02-18 Ambrx Inc Nichtnatürliche kodierte aminosäuren verwendende biosynthetische polypeptide
CN101087875B (zh) * 2004-12-22 2012-08-22 Ambrx公司 氨酰基-tRNA合成酶的组合物及其用途
NZ555386A (en) * 2004-12-22 2011-01-28 Ambrx Inc Formulations of human growth hormone comprising a non-naturally encoded amino acid
US8080391B2 (en) 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
SG160437A1 (en) 2004-12-22 2010-04-29 Ambrx Inc Modified human growth hormone
EP2302032A1 (de) 2005-04-15 2011-03-30 North Carolina State University Verfahren und Zusammensetzungen zur Modulierung von Haftung und Stresstoleranz in Bakterien
JP5362349B2 (ja) 2005-04-15 2013-12-11 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト タンパク質を精製する方法
CN101247821B (zh) * 2005-06-03 2013-01-23 Ambrx公司 经改良人类干扰素分子和其用途
JP2009508470A (ja) 2005-07-21 2009-03-05 アボット・ラボラトリーズ Ｓｏｒｆ構築物並びにポリタンパク質、プロタンパク質及びタンパク質分解による方法を含む複数の遺伝子発現
CN101273140B (zh) * 2005-08-18 2013-01-16 Ambrx公司 tRNA组合物和其用途
NZ567234A (en) * 2005-11-08 2011-09-30 Ambrx Inc Accelerants for the modification of non-natural amino acids and non-natural amino acid polypeptides
DK2339014T3 (en) * 2005-11-16 2015-07-20 Ambrx Inc Methods and compositions comprising non-natural amino acids
ES2529065T3 (es) * 2005-12-14 2015-02-16 Ambrx, Inc. Composiciones que contienen, procedimientos que implican y usos de aminoácidos no naturales y polipéptidos
PT2061878E (pt) * 2006-09-08 2014-04-22 Ambrx Inc Supressor híbrido arnt para células de vertebrados
CN106008699A (zh) 2006-09-08 2016-10-12 Ambrx公司 经修饰的人类血浆多肽或Fc骨架和其用途
CA2662755C (en) * 2006-09-08 2017-04-04 Ambrx, Inc. Suppressor trna transcription in vertebrate cells
EP2076527B1 (de) 2006-10-13 2014-12-10 Novo Nordisk Health Care AG Prozessierende enzyme fusioniert mit basischen proteinanhängern
ES2385114T3 (es) 2007-03-30 2012-07-18 Ambrx, Inc. Polipéptidos de FGF-21 modificados y sus usos
US8114630B2 (en) * 2007-05-02 2012-02-14 Ambrx, Inc. Modified interferon beta polypeptides and their uses
JP5547083B2 (ja) * 2007-11-20 2014-07-09 アンブルックス，インコーポレイテッド 修飾されたインスリンポリペプチドおよびそれらの使用
EP2247743B1 (de) 2008-02-08 2016-04-06 Ambrx, Inc. Modifizierte leptin-polypeptide und deren verwendungen
MX2011000859A (es) * 2008-07-23 2011-02-24 Ambrx Inc Polipeptidos g-csf bovinos modificados y sus usos.
PL2337846T3 (pl) 2008-09-26 2018-06-29 Ambrx, Inc. Mikroorganizmy i szczepionki z replikacją zależną od nie-naturalnych aminokwasów
CA2737026C (en) 2008-09-26 2019-12-24 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
BRPI1014089A2 (pt) 2009-04-02 2016-04-19 Roche Glycart Ag anticorpos multiespecíficos que compreendem anticorpos de comprimento completo e fragmentos fab de cadeia simples
SG10201408401RA (en) * 2009-09-16 2015-01-29 Genentech Inc Coiled coil and/or tether containing protein complexes and uses thereof
RU2012122240A (ru) * 2009-10-30 2013-12-10 Эбботт Лэборетриз Конструкции sorf и экспрессия нескольких генов
TW201138821A (en) 2010-03-26 2011-11-16 Roche Glycart Ag Bispecific antibodies
AU2011291943B2 (en) 2010-08-17 2015-01-22 Ambrx, Inc. Modified relaxin polypeptides and their uses
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
WO2012025530A1 (en) 2010-08-24 2012-03-01 F. Hoffmann-La Roche Ag Bispecific antibodies comprising a disulfide stabilized - fv fragment
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
WO2012085111A1 (en) 2010-12-23 2012-06-28 F. Hoffmann-La Roche Ag Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
CA2861124A1 (en) 2012-02-10 2013-08-15 Genentech, Inc. Single-chain antibodies and other heteromultimers
RU2015100656A (ru) 2012-06-27 2016-08-20 Ф. Хоффманн-Ля Рош Аг Способ получения конъюгатов fc-фрагмента антитела, включающих по меньшей мере одну связывающую группировку, которая специфически связывается с мишенью, и их применения
CN104395339A (zh) 2012-06-27 2015-03-04 弗·哈夫曼-拉罗切有限公司 用于选择并产生含有至少两种不同结合实体的定制高度选择性和多特异性靶向实体的方法及其用途
KR101536963B1 (ko) * 2013-09-17 2015-07-16 재단법인 전주생물소재연구소 식물에서 tev 프로테아제를 대량생산하는 방법 및 상기 방법에 의해 제조된 tev 프로테아제
CN106572974B (zh) 2014-07-15 2021-04-23 生命技术公司 用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法
PE20170950A1 (es) 2014-10-24 2017-07-13 Bristol Myers Squibb Co Polipeptidos del factor de crecimiento de fibroblastos 2 (fgf-21) modificados y usos de los mismos
CN107001482B (zh) 2014-12-03 2021-06-15 豪夫迈·罗氏有限公司 多特异性抗体
ES2963839T3 (es) 2017-02-08 2024-04-02 Bristol Myers Squibb Co Polipéptidos de relaxina modificados que comprenden un potenciador farmacocinético y usos de los mismos
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
CN116144689A (zh) * 2023-03-06 2023-05-23 优睿赛思(武汉)生物科技有限公司 重组猫血清淀粉样蛋白a的表达载体、宿主菌及其制备方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221619A (en) * 1977-11-08 1993-06-22 Genentech, Inc. Method and means for microbial polypeptide expression
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
NZ224663A (en) * 1987-05-28 1990-11-27 Amrad Corp Ltd Fusion proteins containing glutathione-s-transferase and expression of foreign polypeptides using glutathione-s-transferase encoding vectors
US5179007A (en) * 1989-07-07 1993-01-12 The Texas A & M University System Method and vector for the purification of foreign proteins
US5162601A (en) * 1989-11-22 1992-11-10 The Upjohn Company Plant potyvirus expression vector with a gene for protease
GB8927008D0 (en) * 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli

Also Published As

Publication number Publication date
US5532142A (en) 1996-07-02
WO1994018331A2 (en) 1994-08-18
AU6132294A (en) 1994-08-29
ATE294870T1 (de) 2005-05-15
EP0682709B1 (de) 2005-05-04
EP0682709A1 (de) 1995-11-22
WO1994018331A3 (en) 1994-10-13

Similar Documents

Publication Publication Date Title
DE69434358D1 (de) Methode zur herstellung der proteinase nla aus tabak
Heinrikson [20] Applications of thermolysin in protein structural analysis
Dowd et al. Purification and characterisation of a second cathepsin L proteinase secreted by the parasitic trematode Fasciola hepatica
Lee et al. In vitro activation of pro‐phenol‐oxidase by two kinds of pro‐phenol‐oxidase‐activating factors isolated from hemolymph of coleopteran, Holotrichia diomphalia larvae
Uchikoba et al. Cleavage specificity of cucumisin, a plant serine protease
DE69109109D1 (de) Ubiquitinspezifische protease.
Arima et al. Cucumisin-like protease from the latex of Euphorbia supina
Angliker et al. The synthesis of inhibitors for processing proteinases and their action on the Kex2 proteinase of yeast
Dando et al. Human thimet oligopeptidase
CAMARGO et al. Structural features that make oligopeptides susceptible substrates for hydrolysis by recombinant thimet oligopeptidase
CY1110546T1 (el) Μεθοδοι για παραγωγη μεγαλης κλιμακας πεδιου kringle 2 συν πρωτεαση σερινης (k2s) του πλασμινογονου σε προκαρυωτες
Mollay et al. Cleavage of honeybee prepromelittin by an endoprotease from rat liver microsomes: identification of intact signal peptide.
FI880017A (fi) Menetelmä haiman erittämän trypsiini-inhibiittorivarianttien valmistamiseksi ja menetelmässä käytettävä DNA, vektori ja isäntäsolu
DE69906509D1 (de) Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen
Bozner et al. Degradation of collagen types I, III, IV and V by extracellular proteinases of an oral flagellate Trichomonas tenax
De Camargo et al. Influence of the carboxyl terminus of luteinizing hormone-releasing hormone and bradykinin on hydrolysis by brain endo-oligopeptidases.
Rhoads et al. Purification and characterisation of a secreted aminopeptidase from adult Ascaris suum
Kunz Resolution and properties of the proteinases in the larva of the mosquito, Aedes aegypti
Kunz Resolution and properties of the proteinases in adult Aedes aegypti (L.)
Wlodawer Proteasome: a complex protease with a new fold and a distinct mechanism
CA2198966A1 (en) Method for cleaving chimeric protein using processing enzyme
Bania et al. Specificity of an extracellular proteinase from Conidiobolus coronatus and its inhibition by an inhibitor from insect hemolymph
Kurosaka et al. A 38 kDa precursor protein of aqualysin I (a thermophilic subtilisin‐type protease) with a C‐terminal extended sequence: its purification and in vitro processing
Renko et al. Streptomyces rimosus extracellular proteases: 4. Trypsin-like proteinase
Arima et al. Cleavage specificity of cucumisin, a serine protease, with synthetic substrates

Legal Events

Date Code Title Description
8332 No legal effect for de